Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,844 C→G 12.4% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,8440CG12.4% 143.3 / 7.4 69A642A (GCC→GCGrsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (30/29);  new base G (4/5);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-01

CCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCG  >  NC_000913/1708610‑1709079
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
cagagcaaaaGGGGGCAAGCGGAAACAGCAACAGGAAAATGAGGACCCAGAACAACAGGCAGATGCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                                                                                                                                                                                                                         <  2:1202239/232‑1 (MQ=14)
  tggcgaaaGCGCTCAAGCGGGAAATGAGATAGGTAAACGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCAAAAGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                                                                                                                       <  2:182660/234‑1 (MQ=14)
   tgcCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAACGGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGTAGCCGATATTCCCCGTGCCAAAGCACACAAACTGCAGCAGCAACACGCTAATCACGAGccca                                                                                                                                                                                                                                      >  2:1022766/2‑237 (MQ=11)
   cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGACCCAg                                                                                                                                                                                                                                      >  1:32654/1‑239 (MQ=255)
       aaaGCCGGCAAGAAGGAACAGCAACAGGAACATGAGAAAGCAGAACACCAGGCCGAACCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                                                                                                                  <  2:763711/239‑1 (MQ=14)
             cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACACAAGATGGAACAGCAACCGGCTACTGCCGACGCAAACCAACAga                                                                                                                                                                                                                             >  2:1370547/1‑237 (MQ=14)
                            cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTCGATCCGCCCAAAg                                                                                                                                                                                                              >  1:266038/1‑238 (MQ=255)
                            cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACTGGACCAGCAGCAGGCTAATGCGCAGCCACAAAAACAGGTCGGTCCGCGCAAACCCGCCGTCGAGGCGGCTATTCCCCGTGCCAAGGCACGCAAGCCGAAAAAGCAACGGTCGAATACCGCGCCCGaaaacacggctaccccacccaaac                                                                                                                                                                                                              >  2:310571/1‑216 (MQ=2)
                                 ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTCGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGACCAGCAACAGGCTAATGCCGCGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg                                                                                                                                                                                                        >  1:576212/1‑239 (MQ=255)
                                      tagCGGGACCAGAACACCAGGACGCTCGGCGGAAAGCCGCCGGCGAAGCAGCTATCGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                                                                                                                                                   <  2:1380024/237‑1 (MQ=14)
                                       tGCGGACCCGGACAAACGGGTGGATCCGCAGAAAGCCGCCAGTGAAGCCGCAAGTGCCCGGGCCAAAGGGCGGAAGCTGGAACAGCAACAGGCGAAGGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                  <  2:1405592/239‑1 (MQ=14)
                                                  aacaacAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGc                                                                                                                                                                                        >  1:1214964/1‑238 (MQ=255)
                                                  aacaacAGGTCGATCCGCGCAAAGACGCCGTCGAAGCCGCTATTGCTCGTGCCAAAGCGCGCAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGACGACGCCGCTATTGCCCGGGCCAAAGCGGCAAAGAGGGAAGCGCAACAGGCTGATACAGCGCCGCAACAGCAGACCGAGCCGCGGATAGccgccggcacatacacactcgc                                                                                                                                                                                        >  2:995044/1‑222 (MQ=9)
                                                          gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGACGATCCGCGCAAAGCAGCCGTCGCTGCCGCTATTGCCCgcaccca                                                                                                                                                                               >  1:1006619/1‑233 (MQ=14)
                                                            ggaccgaccaaatactgccgccGAAGCCGCTATGGCCCGAGCAAGAGCGGGAAAGCTGGAACAGCAACAGGATAATGCGGAACAAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGGCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCaaa                                                                                                                                                                              <  2:210456/222‑1 (MQ=14)
                                                            cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCaaa                                                                                                                                                                              >  1:845688/1‑238 (MQ=255)
                                                                                ctgAAGCCGCGATTGCGCGTGCCCAAGCGCTCCGGCTGTACAGACGACGGGCTATTGCGGAACCAGCAGACGTGGCCGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCaa                                                                                                                                                         <  2:819935/237‑1 (MQ=14)
                                                                                  gAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGACGCAGCTATTGCCCGCGCTCAAGCCAGCAACCAGGACCAGCAAAAGGCTAATGCGGACCCCGAAGACCAGGGCGATCCGCGCAAAGCCGCCGTAGCCGCGAATATTGCCCGAGCCAAAAGACGCAAGCggcgccagcacac                                                                                                                                                       >  2:823621/1‑228 (MQ=2)
                                                                                       cGCTATTGCCCGCGCAAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCt                                                                                                                                                   <  1:770740/238‑1 (MQ=255)
                                                                                                    gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga                                                                                                                                     >  1:1117526/1‑239 (MQ=255)
                                                                                                    gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGACCGAGCCAAAGCGCGACAGCCGGAACAGCACACGGCCACATCGGAGCCaga                                                                                                                                     >  2:624478/1‑239 (MQ=14)
                                                                                                       aaaGGGAGCAAGCAGGAACAGCAACAGGCAAATAAGGAGCCAGAACAAGAGGCGGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATGGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                  <  2:1292618/239‑1 (MQ=11)
                                                                                                       aaaGCGGGAAAGCTGAAAAAGCAACAGGAAAATACGGACGCAGAACAACAGGTCGATCCGGGAAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                  <  2:1271610/239‑1 (MQ=14)
                                                                                                        aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                  >  2:692520/1‑238 (MQ=255)
                                                                                                         aGCGCGCAAGCTGGAACAGCAACATGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAc                                                                                                                                >  1:304023/1‑239 (MQ=255)
                                                                                                             cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGAGCCCGAACAACAGGTCGATCCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCAGCAAACCGGACCAGCACCCGGCTAACACGGAgaccgcacaaccagt                                                                                                                            >  2:1230272/1‑225 (MQ=14)
                                                                                                             cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGACCAGCAACCGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAAGACCGCAAACGGGAACAGCAACCGGCTATTGAGACACCAGACGAACAggc                                                                                                                            >  2:482862/1‑238 (MQ=14)
                                                                                                              gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                                                                                                                                                                                                                           <  2:188937/127‑1 (MQ=38)
                                                                                                               cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                                                                                                                                                        <  1:1349681/145‑1 (MQ=255)
                                                                                                               cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                                                                                                                                                        >  2:1349681/1‑145 (MQ=255)
                                                                                                                             cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGc                                                                                                             >  1:1020000/1‑239 (MQ=255)
                                                                                                                             cAACAGGCTAATGCCCAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGTCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCTCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                  <  1:122259/218‑1 (MQ=11)
                                                                                                                              aaCAGGCTACTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCt                                                                                                            <  1:1057591/239‑1 (MQ=255)
                                                                                                                                  ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTccc                                                                                                         >  1:1254558/1‑238 (MQ=255)
                                                                                                                                      agtacagagcgaaaacaccAGGGCGACCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCAAAAGGACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                    <  2:1228943/221‑1 (MQ=14)
                                                                                                                                      actGCGGAAGCAGAGGAACAGGCCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                    <  1:1317985/237‑1 (MQ=255)
                                                                                                                                       aTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATGCTCGCAAAGCTGCCGACGAAGCGGTTCTTGCCAGAGCCAAAGCGCGCAGACGGGCACAGGCACCGGCTATTGAGGAGCCCGAAAAAAAGGTAGAtactgcctcttatccatctct                                                                                                    >  2:122259/1‑218 (MQ=2)
                                                                                                                                        tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGaa                                                                                                   >  1:351467/1‑238 (MQ=255)
                                                                                                                                        tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                                                                  <  1:758100/239‑1 (MQ=255)
                                                                                                                                             aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCTGCGCAAAGCCGCCGTCGAAGCCGCt                                                                                             >  1:718555/1‑239 (MQ=255)
                                                                                                                                                  gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc                                                                                        >  1:932648/1‑239 (MQ=255)
                                                                                                                                                         aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTTCCCGAGCCAAAGAGCGCAAAGCGGAACAGCAACCGGCTAATGCGGAGCCAGAAAAACAGGTAGATCCGCCCAAAGCTCACATCGACGCGGCTCCTGCAagccc                                                                                  >  2:960792/1‑236 (MQ=14)
                                                                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACGGGCTAAAGCCGAGCCAGAAAAACAGGTTGATCCGCGCAAAGCCGCCGTCAACGCGGCCATTGCACGCGcc                                                                                 >  2:1151738/1‑238 (MQ=14)
                                                                                                                                                              gcgccgcaaaagcgcgcctgcGAAGACGGTATTACCCGGCACAAAGCAGCCAAGCTGGAACACGAACAGGCTAATGCCGAGCGAGAACAAGAGGTCGACCCCCGCAAAGCCGCCGTCGAAGCCGCTATCACACGAGACAAAGCGCGCAAACGGGAACACCACGCGGCTAATGCGGAGCCAGAAGACGAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGc                                                                            <  2:1303214/219‑1 (MQ=255)
                                                                                                                                                                cccacgcAAAGCTGCCGTCGACGCCGCTATTGGCCGTGCAAAAGAACGACAGCTGGGACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGACGAACCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa                                                                           <  2:1020000/234‑1 (MQ=17)
                                                                                                                                                                 ccGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCACAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                                                                         <  1:73520/239‑1 (MQ=255)
                                                                                                                                                                       aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCAAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGc                                                                    <  1:893171/238‑1 (MQ=255)
                                                                                                                                                                                  cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                         <  1:1014432/238‑1 (MQ=255)
                                                                                                                                                                                          ttatcgtccGCGCCAAAGCGCGCAAGCTGGAACATCAACATGCTAAGGCGGAACCAGAACAGCAGGTCGAGACGCGCAAAGCCGCCGTGGAAGCCGCTATTGACCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCTTCGa                                                                                                    <  2:102157/180‑1 (MQ=255)
                                                                                                                                                                                          cTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                                                                         <  2:592291/214‑1 (MQ=255)
                                                                                                                                                                                               gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                           <  1:1033369/239‑1 (MQ=255)
                                                                                                                                                                                               gCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGCGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                           <  1:701124/239‑1 (MQ=255)
                                                                                                                                                                                                cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                           <  1:692520/238‑1 (MQ=255)
                                                                                                                                                                                                cccGTGCCAAAGCACGCAAGCTGGAACACCAACAGGCTAATGCCGAGCCAGAACAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                           <  1:392149/238‑1 (MQ=255)
                                                                                                                                                                                                  cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACACGCCAAAGCACGCAAGCTGGAACAGCAACAGGCAAAAGCGGTAca                                        >  2:1404639/1‑238 (MQ=255)
                                                                                                                                                                                                    tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCACGCTGGAACAGCAACAGCCTAATACGGTACCag                                      >  1:1019851/1‑239 (MQ=255)
                                                                                                                                                                                                    tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa                                                               >  1:592291/1‑214 (MQ=255)
                                                                                                                                                                                                    cgCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt                                                                                          >  1:102157/2‑187 (MQ=255)
                                                                                                                                                                                                     gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCaga                                     >  1:490427/1‑239 (MQ=255)
                                                                                                                                                                                                        aaaGCGCGCAAGCTGGAACACCAACAGGCTAATGCGGACCCAGAACAGCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga                                  <  1:570341/239‑1 (MQ=255)
                                                                                                                                                                                                        aaaGAACGAAAGCTGGAACAGCAGCGGGTTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCAGTATTGCACGTGCCAAAGAGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGCGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga                                  <  2:266038/239‑1 (MQ=14)
                                                                                                                                                                                                               gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGtt                           >  1:658395/1‑239 (MQ=255)
                                                                                                                                                                                                                  tgggtataagagacgaGGCGAATGCCGAGCCAGATAAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGAAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGAt                        <  2:1277621/224‑1 (MQ=17)
                                                                                                                                                                                                                             cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGc             >  1:552765/1‑239 (MQ=255)
                                                                                                                                                                                                                                      aaTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGc    <  1:478294/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGACCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGATGATCCGCGCAAAGCGGCAGTTAcc   >  2:151257/1‑239 (MQ=255)
                                                                                                                                                                                                                                        tGCGGAGCCAGAACAACAGGCCAATCGGCGCAAAGCCGCCGTCGAAGCCGCTAGTACCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAGGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCcg  <  1:413088/239‑1 (MQ=255)
                                                                                                                                                                                                                                        tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGGGGAACAAGAAGAACAGGTTAATCCGCGCAAACCGGCTGTTGcc   >  2:508813/1‑238 (MQ=255)
                                                                                                                                                                                                                                        tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCcg  <  1:1252393/239‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
CCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCG  >  NC_000913/1708610‑1709079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: