Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,151,689	G→T	28.8%	intergenic (+43/+22)	yegJ → / ← yegK	uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,689	0	G	T	28.8%	28.9 / 4.8	37	intergenic (+43/+22)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (12/14);  new base T (5/6);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

CCCTGGTATTGTTACAAACGTAGAATATGGCCA‑GGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCAT‑TGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151469‑2151924                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                ccttGGTATTGTTAAAAACGAAGAATATGGCCA‑GGAGTGAAAAAAAAAAAAAGAAGATATTACAGACTGGATGTATACGGGGGGGGAAAAAATTTACGGCGGTTATCCCAT‑TGA‑TCTTTTATTGGTCACCTACCAAAAAGAAGAAGCCGACGAGTTAAGAGAAAAATTAGTCCGCTAATCAGCCGTTATATTTATTGTTAAAAC‑GGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATgaga                                                                                                                                                                                                                             <  1:290155/236‑1 (MQ=255)                  aacgaaaaataggCCAGGGAGTGAAAAAAAAAAAAAAAAAATATTTCAGACTGGATTTATACGCGGGGGAAAAAAATTTACGGCGGTTATACATT‑TGA‑TCTTTTATTGGTCACCTACCAAAAAGAAGAAGCCGCCGAGCAAAGAGAAAATTAATTTCGCAAATCAGCCGTTATATTTATTGTTAAAAC‑TGGGGGTTG‑ATTTG‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCcac                                                                                                                                                                                                             <  1:60891/228‑1 (MQ=255)                              tggcaggggggaaaaagaaaaaaaaaaGATATTTCTAACTGGTTTTATCCGGGGGGGAAAAAATTTTACGGGGTTTATCCTTT‑TGA‑TCTTTTAGTGGTCCCCTATCAAAAAGAAGAAGCCGACGAGTAAAGAAAAAAATTGGTCCCATAATCAGCCTTTATTTTTATTGTTAAACC‑GGGCGTTTA‑ATTTG‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc                                                                                                                                                                                                 <  2:171493/218‑1 (MQ=255)                                     gatGGAAAATCAATAATGAAGAGATGTCAGACTTGGCTTATTCGTGCGTGCAGAAAATTTAC‑GCCATT‑TACCATATGATTTCTTTATTGTTCCCATATCAAAAAGAACAAGCGATCGAGTAAAGAGCAAAATAACTTATCGACTGATCGGATATATTTTTTTTTGAACT‑TGGGGGTTG‑AATTG‑TTGTCTAT‑TT‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                           <  2:1033012/236‑1 (MQ=255)                                     atggaaagagcAATAAAAATGATATTTCAGCCTAGAATTGTACGTGTGAGAAAAAATTTGAGGCCGCTTATCACAT‑TGA‑TCGTTTATTGGAAACCTAGCAAAAAAAAGAATCCGGGCAGCAAAAAACAAATCAAGTTCGATAACGGGCTGTAATATTTTTGGTTAAACC‑TGGGGTTTA‑ATTTG‑TTGTCTGT‑GA‑CCTCCATGAGAAACTCGCTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                           <  2:664192/228‑1 (MQ=255)                                                                           tatgCGGGCGGAGAAAAAATGTACGGGTGTTATACCTT‑TAT‑TCATTATTTGGTCCCCTACAAAAAAAAAGAACCCGAGAATGAAAAAAAAAATTTGGTCCGTAAATCGGCTGTTATTTTTTTTTTTTAACC‑TGGCGGTGA‑AATGT‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑c                                                                                                                                                  <  2:101058/239‑1 (MQ=255)                                                                                                              cAT‑TGA‑TCTTTTATTGTCCCCCTAACAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAACCAGCCTTTATATTTATTGTTTAAAC‑GGGCGTTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg                                                                                                               <  1:1236156/239‑1 (MQ=255)                                                                                                              aaT‑TTA‑TCTTTTATGGGCCCCATATCAAAAAAAAGAAGCCGACGATAAAAGAAAAAATGTAGTTCGCTAATCGGCCTTTATTTTTATTTTTTAAAC‑TGGGGGTTA‑ATTTT‑TTGTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                           <  2:94827/164‑1 (MQ=255)                                                                                                                  tGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTT‑AATTA‑TTGTCTTC‑AA‑ACGCCAATAGAAACTCAATCTGCCCACAGCGCCAGAGCTAACGGTTTCTCATCATACGCACGCTCGTTTTCCCCCGGGCTGG‑C‑CAAAAACTTGTTTTACAAATCAGGTGTTAAATCGCgttg                                                                                                            >  2:707072/1‑239 (MQ=255)                                                                                                                  tGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTTTTTTT‑AA‑ACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATTCTCATCCCTACCCCCCTTTAACGCCGGGCTGG‑A‑CAAAAACTGGCTTACAAATTCAAGGAATaaaacaggttg                                                                                                            >  2:646345/1‑231 (MQ=255)                                                                                                                  tGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATTAGAAAATCACTCTGCCCACAGCGCCAAGTCCCACGCTTTCTCATTATCCATACGCTCCTTTACCCCCCGCCTGG‑G‑CCAAAACTGCTTTTACCAATCAGTTTATAAATCGAgttg                                                                                                            >  2:64713/1‑239 (MQ=255)                                                                                                                   gA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTT‑TTTTCTTC‑AA‑CCGCCCTTAGAAAATCAATCCTCCCACAGCGCCAGTGCCCAAGTTTTATCATCCACCGTACGCTCGTTTACCCCCCGGCTGGAA‑CAAAAACGCTTTTAACATTTC‑CGTAATAAAtaacggtgg                                                                                                           >  2:1085913/1‑231 (MQ=255)                                                                                                                   gA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑ACCCCATTAGAAACTCACTCTTACCACAGCCCCAGTGCCAACGCTTTATCATCAATCCTACGCTCGTTTTCCGCCAGGCTCG‑A‑CAAAAAATGCTTTAACAATTCAGGGAATAAATCCAggtgt                                                                                                           >  1:1221147/1‑239 (MQ=255)                                                                                                                          ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑GATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat                                                                                                                                                                                 >  1:94827/1‑165 (MQ=255)                                                                                                                             ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATTAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTatc                                                                                                                                                                                      >  1:1439644/1‑157 (MQ=255)                                                                                                                                gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTT‑TTGTCTTC‑AA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGAGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCACGTAATAAATCGAGTTGTTCCTGCGtt                                                                                                  >  2:1088214/1‑236 (MQ=255)                                                                                                                                 tCACCAAACAAAAAAAAGAGGCCGACGAGCAAAGACAAAAATTAGTTCGTAAACCAGCCTTTATATTT‑TTTTTTAAACGGGGGGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct                                                                                               <  1:646345/238‑1 (MQ=255)                                                                                                                                 gcACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGATA‑AATTT‑TTGTCTTC‑AA‑CCGCCCTGAGAAACTCACTCTTCCCACCGCGCCAGCGCCCACGGTTTATCATAATACGTACGCCCGCTTACCGCCGGGCTGG‑A‑CCAAAACAGCTTTATAAATTCAGGTAATAAATAGAGTTGTTCCTGCGTtgct                                                                                               >  2:1153523/2‑238 (MQ=255)                                                                                                                                        tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTTCCGCCAg                                                                                       >  1:880319/1‑239 (MQ=255)                                                                                                                                             aagaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCCTTAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGTTTTTTCATCATCCGTACGCTCGTTTACCCCCGGGCTGG‑A‑CAAAAACTGGTTTTACAATTCAGGTAATTAAACGAGTTGTTCCTGCGTTGCTGCCGCCCGTccct                                                                                  >  2:311696/1‑237 (MQ=255)                                                                                                                                                    agcggagaagtaaagaaaaaatTTATTTCCTTAACCACCCGTTTTTTTTATTGTTAAAAC‑GGGCGGTTG‑ATTTG‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                           <  1:669190/221‑1 (MQ=255)                                                                                                                                                           gagcaaagagcaaaATTAGTTCGCTAATCAGCAGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTa                                                                    <  1:1003741/239‑1 (MQ=255)                                                                                                                                                                       aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCCTACGCTCGTTTACCGCCCGGGTGG‑G‑CAAAAAATGCTTTAACAAATCAGGTAATAAATCGAGTTGTTCCTGCGCTGCTGCCGCCCGTCCCTTGAAAAACGGGGGTAAGAAAGGcaca                                                        >  1:906293/1‑239 (MQ=255)                                                                                                                                                                              tgcgttAATCGGCTGTTTTATTTTTTTTTTAACC‑GGGGGTTTG‑ATTTG‑TTTTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgaggag                                                 <  1:1332298/234‑1 (MQ=255)                                                                                                                                                                                    aaTCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTTTCTTC‑AA‑ACGCCCTGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGTAACCTCGTTTTCCGCCGGGCCGG‑ACCAAAAACT‑CTTTAAAAATTCAGGGAATAAAATGAGTTGTTCCTGCCGTGCTGCCCCCACTCCCTTTGAAAAAGGGGTAAAGAAAGGCACATGAGGAGAAatat                                           >  1:583168/1‑239 (MQ=255)                                                                                                                                                                                       ccacccttaatttttttttttTAAC‑CGGGGGTTA‑AATTT‑TTTGCCTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc                                         <  1:140721/228‑1 (MQ=255)                                                                                                                                                                                            ctttttttttttttgtttaaggggCGGTTA‑AATTT‑GTGTTTTT‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGTTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                     <  1:166227/217‑1 (MQ=255)                                                                                                                                                                                            caattatttatt‑TTTAAAC‑GGGGGGTTA‑AATTT‑TTTTCCTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGCACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                     <  1:1432895/233‑1 (MQ=255)                                                                                                                                                                                               atatTTATTGTTTAAAC‑TGGCGGTTT‑AATTG‑TTGTCTTT‑AA‑CCCCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGGTGG‑A‑CAAAAACTACCTTTACAAtttcggg                                                                                                                          >  1:1413158/1‑144 (MQ=255)                                                                                                                                                                                                  tttattGTTTAAAC‑TGGCGGTTG‑AATTG‑TTTTTTTC‑AA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCCGGGTGG‑A‑CAAAAACTGCTTTTACAATTCCGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCCTTGGAAAAAGGGGGAAAGAAAAGCACATGAGGAGAAATATTCAGCCTATTTAAc                             >  2:1420757/1‑239 (MQ=255)                                                                                                                                                                                                        ttttAAAC‑GGGGGGTTGAAATTT‑TTTTCACC‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg                        <  1:752739/238‑1 (MQ=255)                                                                                                                                                                                                        ttttAAAC‑AGGCGGTTG‑AATTA‑TTGTCTAC‑AAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATACAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg                        <  1:568031/238‑1 (MQ=255)                                                                                                                                                                                                        tgtaaaaC‑GGGGGTTTG‑ATTTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt                       <  1:1028394/235‑1 (MQ=255)                                                                                                                                                                                                         tttAAAC‑TGGGGGTTG‑AATTT‑TTTTTTTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg                        <  1:1308690/238‑1 (MQ=255)                                                                                                                                                                                                           tAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACCCTCCTTTACCGCCCGGCCGG‑A‑CAAAAAATTCTTTTACAATTCAGGGAATAAATCGAGTTGTTCCTGCGGTGCTTCCGCCCGTCCATTGGAAAAAGGGGGAAAAGAAGGCACATTAGGAGAATTATCCAGCCTATTTAAAGCCAGTCg                     >  1:925522/1‑238 (MQ=255)                                                                                                                                                                                                                  ggCGGTTG‑AATTG‑TTTTCTTC‑AA‑ACGCCCTGAGAAACTCACTCTGCCCACAACGCCCGGGCCCACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTTAGGTAATAAATCGAGTTGTTCCTGCGTTGCTTCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACCTGAGGAGGATTATCCAGCCTATTTAACGCCCGTCGCTGGAt               >  2:936614/1‑238 (MQ=255)                                                                                                                                                                                                                     ggTTT‑AATTGTTTGTCTAA‑AC‑CCGCCATGAGAAACTCCCTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc             <  1:1040223/238‑1 (MQ=255)                                                                                                                                                                                                                                 ttgtCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  1:198900/1‑238 (MQ=255)                                                                                                                                                                                                                                   gtCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  1:441151/1‑236 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                CCCTGGTATTGTTACAAACGTAGAATATGGCCA‑GGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCAT‑TGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151469‑2151924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑