BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,465	T→G	8.3%	M2R (ATG→AGG)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,465	0	T	G	8.3%	223.6 / 2.7	118	M2R (ATG→AGG)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (41/63); new base G (2/10); total (44/74)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 120 total aligned reads displayed.

TGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATT  >  NC_000913/3206230‑3206691

tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTACAGA‑CAAATAC‑AAAAAAAGGAGTTGTCat‑tatggg                                                                                                                                                                                                                                                       >  1:1280570/1‑237 (MQ=255)

    tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGTCGCAATATTCATTCTTATTGTCGCGTTGATTCACGAACTGGTAATGACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGa                                                                                                                                                                                                                                                    <  2:55961/238‑1 (MQ=255)

            aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAA‑ATTTTTCAGACCAAAAAC‑AAAAA‑TTGAGATGTCat‑gatggagggaaaaaatat                                                                                                                                                                                                                                           >  2:971447/1‑225 (MQ=255)

              cTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGaaa                                                                                                                                                                                                                                                  <  1:288314/230‑1 (MQ=255)

                  tCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑a                                                                                                                                                                                                                                <  1:1433478/239‑1 (MQ=255)

                       aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                     <  2:597756/236‑1 (MQ=255)

                       aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                     <  1:588961/236‑1 (MQ=255)

                        tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAGA‑CAAA‑AA‑AAAAAAAGGAGTTTCCAT‑GGTGAGCGgaaagaaaaaacaaaaatcaac                                                                                                                                                                                                                               >  2:1083770/1‑220 (MQ=255)

                        tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑CAAATAA‑AAAAAATTGAGTTGGCAT‑GATGAGCGGAAAGAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GGA‑Gt                                                                                                                                                                                                                >  1:398496/1‑239 (MQ=255)

                         cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑Gc                                                                                                                                                                                                                    <  1:844009/235‑1 (MQ=255)

                         cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTg                                                                                                                                                                                                               <  1:1290429/239‑1 (MQ=255)

                             aaTTATCCGCAGGTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATa                                                                                                                                                                                                           <  2:1382686/239‑1 (MQ=255)

                                  tCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTg                                                                                                                                                                                                      <  1:1083770/239‑1 (MQ=255)

                                                 aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTTAGA‑CAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGAAAAAAGTG‑ACAGAGATGTTCGc                                                                                                                                                                                       >  2:894581/1‑238 (MQ=255)

                                                 aGCGAAGGGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCGGGCGCAATATTCATTCTTATTTCCGCGTTGATTCACGAACTGTTAATTACTTATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCt                                                                                                                                                                                      <  2:800534/239‑1 (MQ=255)

                                                   cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑ATTTTTCACA‑AAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCACGTAACTAA‑TTG‑GCAGAGAtttagcgat                                                                                                                                                                                     >  2:147067/1‑231 (MQ=255)

                                                   cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCTA‑CAAATTC‑AAAAAATTGAGTTTCACT‑GATGAGCGCAAATAAAAAACAC‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAAATT‑ACATAGATTGTTTCTaa                                                                                                                                                                                    >  1:800534/1‑239 (MQ=255)

                                                        tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTa                                                                                                                                                                              <  1:1082447/239‑1 (MQ=255)

                                                                  aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTTCAAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTA‑ACAGAGTTTGTCGTTAAC‑TTTACCGCCATG‑At                                                                                                                                                                   <  2:1306196/233‑1 (MQ=255)

                                                                  aaaaCATTGCCA‑TTCCCTGTTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAATTGTTAATTACATATT‑CACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑AAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGTTAAC‑TTTACCGCCATG‑At                                                                                                                                                                   <  1:1220250/239‑1 (MQ=255)

                                                                  aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAGA‑CAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAAAGAATGTCGCTAAC‑TTTAACGCCATG‑At                                                                                                                                                                   >  1:251319/1‑239 (MQ=255)

                                                                  aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAGA‑CAAAAAC‑AAAAAAAGGAGGTGCCAT‑GATGAGCGGAAGAAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTT‑ACAGAGATTGTGGCTAAA‑TTTATCCCCCTG‑At                                                                                                                                                                   >  1:59610/1‑239 (MQ=255)

                                                                    aaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑AAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑Attt                                                                                                                                                                 <  1:1192224/239‑1 (MQ=255)

                                                                         tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAA‑TTTTTTCAGA‑AAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGGAAAAAATT‑AAAGAGATTGGTGCTCAC‑TTTTACGCCATG‑Aatttttc                                                                                                                                                             >  1:642912/1‑234 (MQ=255)

                                                                           ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAGA‑CAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAA‑‑‑‑CAA‑‑‑GCAAAAAGCA‑GCGGATAAGTTG‑ACAGAGATTTTTGCTAAA‑TTTACCCACATG‑Attt                                                                                                                                                                 >  1:558935/1‑239 (MQ=255)

                                                                             ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GCGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                         <  1:677346/237‑1 (MQ=255)

                                                                                 cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAAA‑CAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ACGAATAAGATT‑AGAGAGAATGTGGGTCAC‑TTTACCGCCCTG‑ATTTCTATCCGAATGc                                                                                                                                                     >  2:970454/1‑239 (MQ=255)

                                                                                          ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑CAAAAAA‑AAAAAATCGAGTTTTCCT‑GATGTGGGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAATTT‑ACAGAGAATGTCGCTAAA‑TTTAACTCCATG‑TTTTTTACCCGCATGACcgatgtgg                                                                                                                                            >  1:623602/1‑236 (MQ=255)

                                                                                           gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGA‑TTTTTTCAAA‑AAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GCA‑CAGAATAAGTTT‑TCAGAGATTGTCGTTACC‑TTTATAGCCCCG‑CTTTCTATCCGAATGccccgggacgt                                                                                                                                           >  2:968220/1‑230 (MQ=255)

                                                                                            cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGA‑CAAAAAA‑AAAAAATGGAGTTGCCAT‑GATGAGCGAAAGAAAAAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTG‑ACAGAGATTGGCGCTAAC‑TTTTCCGCCATG‑ATTTTTACCCCAATGCCtgattaaggg                                                                                                                                          >  1:1349641/1‑233 (MQ=255)

                                                                                                 aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAA‑TTGTTCAAAA‑AAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAG‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑ACA‑TGAAATAAGTGG‑AAGGGAATTGGCGCAAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                     <  2:1291506/234‑1 (MQ=255)

                                                                                                   ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑CAAATAC‑AAAAACTGGGGTTTCCAT‑GATGAGCGAGAGAAATAAGCAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAATTT‑ACAGAGATTGTCGCTAAA‑TTTACCGCCATG‑ATTTCTACCCCCATGCCTGTTGGCGGGGGGGCTa                                                                                                                                   >  1:1245946/1‑239 (MQ=255)

                                                                                                         tttATTTTAGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGAAAAAAAAATAAAACCACTTTTTAAGTGTGAGTTTGTGGCGTGGAA‑TTTTTCAAGA‑AAAAAAC‑AAAAACTGGAGTTGCCAT‑GTGAAGAAAAATTAATAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCCCTAAC‑TTTTCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                            <  1:968220/238‑1 (MQ=255)

                                                                                                         cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTTCCAGA‑CAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                            <  1:328413/239‑1 (MQ=255)

                                                                                                           tatttactgtttGATTCCCTAACTGTTAATTACATATT‑CGAGGTGGAAAAAAATAGAAAACCATGTTTGTATTGGTGGTTTGGGGCGGAAAA‑TTGTTCCTGA‑AAAAGAC‑AAAATGTGGACGTTCACT‑GATGAGGGAGAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑ACA‑TTCAAAAGGTTG‑ACAGAGATTGCCGCAAGG‑TTTACCGCCATG‑ATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACT‑aaca                                                                                                                          <  2:831787/229‑3 (MQ=255)

                                                                                                           tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTTACA‑CAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑ACA‑GAGAAACAGATT‑AAAAAAATTGTAGGTAAA‑TTTTAAGCCATG‑AGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACC‑aaac                                                                                                                          >  2:958061/1‑238 (MQ=255)

                                                                                                           tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCCGA‑CAAAAAC‑AAAAAATTGAGTTTGCCT‑GATGAGAGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑ACA‑GAGAAAAAATTG‑ACAGAGATTGTTGCTAAA‑TTTAACGCCAAG‑ATTTTTACCCCAAAGCCCGATTACGGGGGGGATAAACT‑aaaa                                                                                                                          >  1:459018/1‑239 (MQ=255)

                                                                                                           tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAA‑ATTTTTAAGA‑CAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑AAA‑ATGAAAAAGATG‑GCAGAGAGTGTTGCTAAC‑TATACCGCCCTG‑ATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACA‑aaaa                                                                                                                          >  2:475605/1‑239 (MQ=255)

                                                                                                             ttttCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTACGA‑AAAAAAC‑AAAAAATGGAGTTTTCAT‑TGTGGGAGGAAAGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAAAAAATTG‑ACAAAGATTGTGGCGTAC‑TTTTTCGCCCTG‑ATTTCTTTCCGAATACCTGATGACGTGGGGGGTATACA‑aaaaaa                                                                                                                        >  2:582594/1‑237 (MQ=255)

                                                                                                             gtcagaggtggataagaaacaggTTATTTACATATT‑CGCGGTGGAAAAAAATATAAAACCACATTTTAAGTGTTGGTGTGTGGCGGTGAA‑TTTTACAAGA‑CAAATAC‑AAAAACTGGAGTTTCCAT‑GATGAGCAAAATTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑TTA‑TTAAATAAGTTG‑ACAGAGATTGTCGTAAAT‑TTTACCGCCATG‑ATTTCTACCCTAATGCCTGATGACGTGGTGGATAAACT‑aaaa                                                                                                                          <  2:660336/215‑1 (MQ=255)

                                                                                                                  gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGA‑TTTTTTCAGA‑CAAAAAA‑AAAAACAGTAGGGTCCTG‑TATTGGGGGAAGTTATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAAATT‑GCAGAAATTGTCGCTAAC‑ATTACCCCCCAG‑AATTCTTCCCAAATGCCTGATTACGCGGTGGATAAATT‑aaaaagagtta                                                                                                                   >  2:1151044/1‑232 (MQ=255)

                                                                                                                  gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑CAAAAAA‑AAAAAAAGGAGTTGGCAT‑TATGAGGGGAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAGATG‑GCAGAGAATGTTGCTAAA‑TTTACCACCCTG‑ATTTCTACCCGAATACATGATGACTGGGGGGGTAAACA‑aaaaaaaataa                                                                                                                   >  2:1102120/1‑232 (MQ=255)

                                                                                                                    gtttataagagacgggTTAATTACAGATT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTCCCAGA‑CAAATAC‑AAAACCTGGAGTGGCAAT‑GATGAGGAAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑CAAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg                                                                                                                 <  1:1826/224‑1 (MQ=255)

                                                                                                                     tttaTTCAGGAATTTTTAATTAAATATT‑CAGGGTGCAAAAAAATAAAAAACCCTATTTTTGGTGGTGGTTTGTGGGGGTGAA‑TTTTTCCAAA‑AAAATAA‑AAAAACGGGATTTGCCAT‑GGTGAGGGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg                                                                                                                 <  2:1245946/235‑1 (MQ=255)

                                                                                                                      gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAA‑TTTTTACAGA‑CAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCG‑GTGAATAAGTTG‑ACAGAGTTTTTCACTAAC‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg‑g                                                                                                               <  2:1280570/231‑1 (MQ=255)

                                                                                                                                  tGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑CAAATAA‑AAAAAATGGAGGTGTCAT‑GATGAGCGAAAGGAATAAATAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GGA‑GGGAATAAGATG‑ACAGAGATTTGCGCTAAC‑TTTAACCCCCAG‑ATTTCTACCCGCAAGCCTCATGACGTGGGGGAAAAAAT‑AAAAAAACTaaaaggcggccaaaagt                                                                                                    >  1:697927/1‑224 (MQ=255)

                                                                                                                                    ttAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAgaga                                                                                                                                                                                              <  1:697647/149‑1 (MQ=255)

                                                                                                                                    taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                     <  1:263711/202‑1 (MQ=255)

                                                                                                                                      aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTACCAGA‑CAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGtc‑gtcg                                                                                             <  2:595367/239‑1 (MQ=255)

                                                                                                                                        ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCAAGA‑CAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGAt                                                                                           <  1:1068744/239‑1 (MQ=255)

                                                                                                                                            atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑AAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAAAAAATTG‑AAAGAGAGTGTCGCTAAC‑TTTACCGCCATG‑ATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACT‑AAAAAACCTAAAG‑GATACCGAAAAGTC‑CTCGGTgggg                                                                                       >  1:631210/1‑239 (MQ=255)

                                                                                                                                            atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGA‑CAAAAAC‑AAAAAATTGAGTTTTCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAGTTG‑AAAGAGATTGTCGCTAAC‑TTTAACGCCCTG‑ATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAAAT‑AAAAAAGCTAAAG‑GATGACGCAACGCA‑GGCGATgggg                                                                                       >  1:1388078/1‑239 (MQ=255)

                                                                                                                                            atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTATAA‑CAAATAA‑AAAAACAGGAGTTGGCAT‑TATGAGGGGAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑GGGGAAAAGTTT‑AGAGGGAATGTTGCTACA‑TTTATACCCATGAATTATTACCCAAAAGCC‑GGTGTTGTGGGGGATAAAAC‑AAAACAGCTAAAA‑AACTTCGGAAAGTC‑GTCGACgggg                                                                                       >  2:1019021/1‑239 (MQ=255)

                                                                                                                                            atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAA‑TTTTTTCAGA‑CAAATAC‑AAAAAAGGGGGGTGCCGT‑GGTGGGCTGGAGTAATAAAAAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GAA‑ATAACTAAGTTT‑TCTGACATTTGGGCTAAA‑TTTAAATCAATG‑CGTTCTTCCCCAACCCCTGAGTGCGCCGGGGGTAAAAA‑AAAACAGCTAAAGAGTTTCCTGAAC‑TC‑GTCGATgggg                                                                                       >  2:229326/1‑239 (MQ=255)

                                                                                                                                            atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAA‑TTTTTTTAGA‑CCAAAAA‑AAAAACTAGAGGTGGCAA‑GAAGAGGGAAAAAAATAAGCAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑GAGAATAAATTG‑TCCGAGAATGTGGCTAAA‑TTTACCTCCATC‑AATTTTTACCGAATGCCTGGTGTCGATGGGGATAAACT‑AAAACAACCAAAA‑Gaatgcgcaaacacgtacacgggg                                                                                        >  2:794496/1‑217 (MQ=255)

                                                                                                                                            atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGAT‑TTTTTCCAGA‑AAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGa                                                                                                        <  1:1049051/223‑1 (MQ=255)

                                                                                                                                             tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAA‑TTTTTACATA‑CAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTGG‑ACAGAGATTGTCGTTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  1:1403772/239‑1 (MQ=255)

                                                                                                                                             tatT‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGGGGTGAA‑TTTTTCCAGA‑AAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  1:469404/239‑1 (MQ=255)

                                                                                                                                             tatT‑CACGGTGGAAAAAAATATAAAACCAAATTTTTAGGGGTAGTTTGGGGCGGTAAA‑TTTTTCCAGA‑CAAAAAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  2:1332086/239‑1 (MQ=255)

                                                                                                                                             tatT‑CACGGTGGAAAAAAATATAAAACAACATTTTTAGTGGTAGTTTGGGGGGGTAAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCTAT‑GATGAGCAAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  1:344668/239‑1 (MQ=255)

                                                                                                                                             attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  1:1064968/237‑1 (MQ=255)

                                                                                                                                              tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTTCAAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTA‑ACAGATATTGCCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  2:251319/233‑1 (MQ=255)

                                                                                                                                              atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAGA‑AAAAAAA‑AAAAAATTGAGTTGGCCT‑GGTGGGTGGAAAAAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑ACA‑AGGGATAAATTG‑GAAGAGATTGTTGCTaa                                                                                                                                                                                    >  2:697647/1‑149 (MQ=255)

                                                                                                                                              atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAAA‑CCAAAAC‑AAAAAATGGGGTTTGTTT‑GGTGGGCGCAAGTAATAAACAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAATTG‑CAAGAGATTTTTGCTAACATTAACCACCA‑G‑ATTTTTACCCGAATGCCTGCTGATGTGGGGGATTAAAT‑AAAAAAGCTTAAG‑GCTACAGaaaactagtgggtggggga                                                                                      >  2:748633/1‑223 (MQ=255)

                                                                                                                                              atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑ATTTTTTAAA‑CAAAAAC‑AAAAACAGGAGGTTGCAT‑GGTGAGCGGAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑AAA‑AGGGATAAGATGTACA‑AGAATGTGGCTAAA‑TATACCCCCATA‑ATTTTTTACCGAAAGTATGTTGTAGGGGTGGATTAAAC‑AAAACAACTTAAA‑GAGGCCGCAACCGC‑GTCGGTggggga                                                                                     >  2:91747/1‑237 (MQ=255)

                                                                                                                                              atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑AAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGaa                                                                                     <  1:1151052/239‑1 (MQ=255)

                                                                                                                                              atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAAA‑CAAAAAA‑AAAAAATGGAGATGCCAT‑GATGAGCGGAAGTAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAAAAAAATG‑AAAGAGATTGTCGGCAAA‑TTTACCCCCATG‑ATTTTTACCCCAATGCCTGATGAGGGGGGGGGTAAAAT‑AAAAAAGCTAAAG‑Gaaagtctcttttacacatatccgc                                                                                       >  1:660336/1‑215 (MQ=255)

                                                                                                                                              aattacgggTGGAAAAAAAAAAAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTTCCAGA‑CAAAAAC‑AAAACCTGGAGTGGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑TTAAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                      <  1:636384/232‑1 (MQ=255)

                                                                                                                                                   aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGA‑TTTTTTCAAA‑CAAAAAA‑AAAAAATTGAGTTGCCCT‑GATTGGGGGAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAATTG‑ACAGAGATTTTCGCTAAC‑TTTTCCGCCATG‑ATTTTTACCCGAATGCCTGATTACGTGGGGGGTAAAAT‑AAAAAAGATAAAG‑GATGCCGAAAAGTC‑GGCGATGGGGGAAAtt                                                                                 >  1:1201523/1‑239 (MQ=255)

                                                                                                                                                    cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAAA‑CAAAAAA‑AAAAAATTGAGGTGGCCT‑GATTGGGGGAAGTAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAAATT‑ACAGAGAATGTGGCTAAC‑TTTACCCCCAAG‑ATTTTTATCCCGATGGACGAAAACGGGGGGGGTAAAATAAAAACACTTAAAG‑AATCCGGAAACGTG‑TCCGTGGGGGAAAAta                                                                                 >  2:487241/1‑238 (MQ=255)

                                                                                                                                                     gagaaaaaaaaaTA‑AAAACCAAATTTTTGTGGGTGGTTTGTGAGGGTGAA‑TTTTTCAAAA‑AAAATAA‑AAAAACGGGAGTTGCCTG‑GATGAGAAAAATAAAAAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAGGTTG‑ACAGAGATTGCCGATAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                               <  2:459018/232‑1 (MQ=255)

                                                                                                                                                      ggggCAAAAAAAAAAAAACCAAATGTTTAGTGGTAGTTTGGGGCGGTGAA‑TATTTCCAGA‑AAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAATAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAGATTGTCGCAACC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAAGTAt                                                                               <  2:576654/236‑1 (MQ=255)

                                                                                                                                                      gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                               <  1:958061/238‑1 (MQ=255)

                                                                                                                                                      gTGGAAAAAAAAAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAA‑TTTTTCAAGA‑AAAATAA‑AAAACCTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTTTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                               <  2:623602/238‑1 (MQ=255)

                                                                                                                                                       tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑ATTTTTCAGA‑CAAAAAC‑AAAAAATGGAGGTGCCAT‑GATGAGGGGAAATAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑GCAGAGAATGTCGCCAAC‑TTTACCGCCATG‑ATTTCTACCCGCATGCCTTATTACGTGGTGGAGAAAAT‑AAAACAACTAAAA‑GATGCCGAAACGtc‑gtcgcctgccccttatacac                                                                             >  2:1049051/1‑223 (MQ=255)

                                                                                                                                                       tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAA‑TTTTCCCAGA‑AAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACATCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATc                                                                              <  1:134290/238‑1 (MQ=255)

                                                                                                                                                            aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAG‑ATTTTCCAAA‑CAAAAAA‑AAAAAATGGAGTTGACCT‑GGTGGGCGGAAGAAAAAAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑ACA‑AAGCAAAAGATG‑GCAGAGATGTTCGATCAA‑TTTACTGCCCTG‑ATTTCTAACCGAAAGCCCGACTACGGGGTGGAGAAACA‑AAAACAGCCAAAG‑GAGGCCCACA‑GTCAGTCGGTGGGGAAAATTATCTACCa                                                                         >  2:1323481/1‑238 (MQ=255)

                                                                                                                                                               aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAA‑TTTTTCCAAA‑AAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCAAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                    <  2:1201523/239‑1 (MQ=255)

                                                                                                                                                               aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAA‑TTTTCCCAGA‑AAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑TGGAATAAGTTG‑ACAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                    <  1:40417/239‑1 (MQ=255)

                                                                                                                                                                           caTTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCAGA‑CAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGATG‑ACAGAGATTGTCGCCAAC‑TTTACCGCCCTG‑ATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACT‑AAAACAGCTAAAA‑GATGCCCAAACGAC‑GTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                        >  1:957057/1‑238 (MQ=255)

                                                                                                                                                                           caTTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTTCACA‑CAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAAATG‑ACAAAGATTGTCGCCAAC‑TTTTCCg                                                                                                                                                                           >  1:77967/1‑129 (MQ=255)

                                                                                                                                                                                        ggTTTGGGGTGGAAAA‑TTTTACAAAA‑AAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGGAATAAGTTG‑ACAGAGATTGCCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                           <  2:652970/238‑1 (MQ=255)

                                                                                                                                                                                        aGTTTGTGGCGGTAAA‑TTTTTCAAAA‑AAAATAA‑AAAAACTGGAGTTGCCAT‑GATGGCCGAAAATAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATGGTCGCTACC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                           <  1:1151044/239‑1 (MQ=255)

                                                                                                                                                                                              tgGCGGTGAA‑TTTTTCCAGA‑CAAAAAC‑AAAAATTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGAc                                      <  1:669602/238‑1 (MQ=255)

                                                                                                                                                                                                gCGGTAAA‑TTTTCCAAGA‑CAAATAA‑AAAAACTGGAGTTGCCAA‑GATGAGCAAAAGTAATAAGCAAAGGGC‑AAGGG‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATGGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                           <  2:558935/238‑1 (MQ=255)

                                                                                                                                                                                                    taaa‑TTTTCCCAGA‑AAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGA‑ACAGAGATTGTCGCTACC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                <  1:147067/236‑1 (MQ=255)

                                                                                                                                                                                                           ttaccAAA‑CAAAAAA‑AAAAACTGAGTTTCCCAG‑GAAGAGCAAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑CGA‑GGAAATAAGTTG‑AAAGAGTGTGTCGTAAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                         <  2:1232761/236‑1 (MQ=255)

                                                                                                                                                                                                                        tAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca             <  1:475605/239‑1 (MQ=255)

                                                                                                                                                                                                                         taaaaaaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca             <  1:642776/236‑1 (MQ=255)

                                                                                                                                                                                                                            aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGATTTTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg          <  1:795382/239‑1 (MQ=255)

                                                                                                                                                                                                                              aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg        >  2:1250640/1‑239 (MQ=255)

                                                                                                                                                                                                                               accgggAGTTGCAAT‑GATGAGGGAAAGAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGAAAAAGGTTG‑AAAGAGATTGCCGCTACC‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  2:59610/235‑1 (MQ=255)

                                                                                                                                                                                                                               aatgtgAGTTGCCGT‑GAGGAGAGAAAGAAAAAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GGTAATAAGTGG‑ACAGAGAGTGGCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  2:212011/234‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAAAAAGTTG‑ACAGAGTTTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  2:1349641/239‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  1:615170/239‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  1:794496/239‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGTTGGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  1:905093/239‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  1:582594/239‑1 (MQ=255)

                                                                                                                                                                                                                               aaCTGAAGTTGCCAT‑GATGAGCGAAATTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAATAAGTTG‑GCAGAGATCGTACCTAAT‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg       <  1:793625/239‑1 (MQ=255)

                                                                                                                                                                                                                                aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga      >  2:931448/1‑239 (MQ=255)

TGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAA‑TTTTTCCAGA‑CAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATT  >  NC_000913/3206230‑3206691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`