BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,481	G→A	23.7%	K7K (AAG→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,481	0	G	A	23.7%	187.8 / 26.6	122	K7K (AAG→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (22/69); new base A (26/3); total (48/74)
Fisher's exact test for biased strand distribution p-value = 3.02e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC  >  NC_000913/3206247‑3206714

 tCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑a                                                                                                                                                                                                                                                      <  1:1433478/239‑1 (MQ=255)

      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                                           <  2:597756/236‑1 (MQ=255)

      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                                           <  1:588961/236‑1 (MQ=255)

       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATAAAAATAC‑AAAAAAAGGAGTTTGCAT‑GATGAGGGAAAGAAATAAGAAA‑‑‑‑A‑A‑‑‑gtctct                                                                                                                                                                                                                                             >  2:288314/1‑234 (MQ=255)

       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATTGAGTTGGCAT‑GATGAGCGGAAAGAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GGA‑Gt                                                                                                                                                                                                                                      >  1:398496/1‑239 (MQ=255)

        cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑Gc                                                                                                                                                                                                                                          <  1:844009/235‑1 (MQ=255)

        cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTg                                                                                                                                                                                                                                     <  1:1290429/239‑1 (MQ=255)

          ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTTGGATTGACAC‑TAGGAGGGAAAGTAATAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑ATGaa                                                                                                                                                                                                                                   >  2:601997/1‑239 (MQ=255)

                 tCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTg                                                                                                                                                                                                                            <  1:1083770/239‑1 (MQ=255)

                                aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGAAAAAAGTG‑ACAGAGATGTTCGc                                                                                                                                                                                                             >  2:894581/1‑238 (MQ=255)

                                aGCGAAGGGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCGGGCGCAATATTCATTCTTATTTCCGCGTTGATTCACGAACTGTTAATTACTTATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCt                                                                                                                                                                                                            <  2:800534/239‑1 (MQ=255)

                                  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAAAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCACGTAACTAA‑TTG‑GCAGAGAtttagcgat                                                                                                                                                                                                           >  2:147067/1‑231 (MQ=255)

                                       tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTa                                                                                                                                                                                                    <  1:1082447/239‑1 (MQ=255)

                                              cGCAAAACATTCCCA‑TGCCTTGCTTCGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCAAACTTTTAATTACATATA‑CTCGGTGGCAAAAAATAAAAACCAAAATTTTTGGTGGTAGTTTGGGGAGGTGAATTTTTCCAGAAAAAAAC‑AAAAACTGAGGTTGCAAT‑GATGAGCGAAAGTAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAAC‑TTTACCGCCAt                                                                                                                                                                                             <  1:618457/239‑1 (MQ=255)

                                                 aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTA‑ACAGAGTTTGTCGTTAAC‑TTTACCGCCATG‑At                                                                                                                                                                                         <  2:1306196/233‑1 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAAAGAATGTCGCTAAC‑TTTAACGCCATG‑At                                                                                                                                                                                         >  1:251319/1‑239 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAAAGGAGGTGCCAT‑GATGAGCGGAAGAAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTT‑ACAGAGATTGTGGCTAAA‑TTTATCCCCCTG‑At                                                                                                                                                                                         >  1:59610/1‑239 (MQ=255)

                                                   aaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑Attt                                                                                                                                                                                       <  1:1192224/239‑1 (MQ=255)

                                                        tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAAAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGGAAAAAATT‑AAAGAGATTGGTGCTCAC‑TTTTACGCCATG‑Aatttttc                                                                                                                                                                                   >  1:642912/1‑234 (MQ=255)

                                                          ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAA‑‑‑‑CAA‑‑‑GCAAAAAGCA‑GCGGATAAGTTG‑ACAGAGATTTTTGCTAAA‑TTTACCCACATG‑Attt                                                                                                                                                                                       >  1:558935/1‑239 (MQ=255)

                                                            ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GCGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                                               <  1:677346/237‑1 (MQ=255)

                                                            a‑TTCCCTGCTCTGGCACAATATTCTTTCTTTTTTTCGCGTTGATTCACGAACGGTTAATTACATTTT‑CACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTACC‑TTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                              <  1:482289/239‑1 (MQ=255)

                                                                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ACGAATAAGATT‑AGAGAGAATGTGGGTCAC‑TTTACCGCCCTG‑ATTTCTATCCGAATGc                                                                                                                                                                           >  2:970454/1‑239 (MQ=255)

                                                                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GCA‑CAGAATAAGTTT‑TCAGAGATTGTCGTTACC‑TTTATAGCCCCG‑CTTTCTATCCGAATGccccgggacgt                                                                                                                                                                 >  2:968220/1‑230 (MQ=255)

                                                                            gcAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATT‑CAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAAACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                <  1:970454/238‑1 (MQ=255)

                                                                                tatTCTATCTTATTTTCGAGTTTATTCACGAACTGTTAATTAAATATC‑CAGGGTGCCAAAAAATATAAAACCTCATTTGTAGGGGAAGTTTGTGGGGGTGAATTTTCCAAGACAAATAC‑AAAACCTGGAGTTGCCAT‑GAGTAGCAAAAATAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAATTTGCCGCTATC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  2:1286999/239‑1 (MQ=255)

                                                                                aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAATTGTTCAAAAAAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAG‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑ACA‑TGAAATAAGTGG‑AAGGGAATTGGCGCAAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  2:1291506/234‑1 (MQ=255)

                                                                                  ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGGGTTTCCAT‑GATGAGCGAGAGAAATAAGCAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAATTT‑ACAGAGATTGTCGCTAAA‑TTTACCGCCATG‑ATTTCTACCCCCATGCCTGTTGGCGGGGGGGCTa                                                                                                                                                         >  1:1245946/1‑239 (MQ=255)

                                                                                        tttATTTTAGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGAAAAAAAAATAAAACCACTTTTTAAGTGTGAGTTTGTGGCGTGGAATTTTTCAAGAAAAAAAC‑AAAAACTGGAGTTGCCAT‑GTGAAGAAAAATTAATAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCCCTAAC‑TTTTCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                                  <  1:968220/238‑1 (MQ=255)

                                                                                        cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                                  <  1:328413/239‑1 (MQ=255)

                                                                                         ttATTTTCGCGTTGATTCACGAACTGTTAATTACATTGT‑CACGGTGGAAAAAAATAGAAACCCACATTTGTAGTGGTATTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGACTGATGACGTGGTGGATAAACT‑aaa                                                                                                                                                 <  1:359612/239‑1 (MQ=255)

                                                                                          tatttactgtttGATTCCCTAACTGTTAATTACATATT‑CGAGGTGGAAAAAAATAGAAAACCATGTTTGTATTGGTGGTTTGGGGCGGAAAATTGTTCCTGAAAAAGAC‑AAAATGTGGACGTTCACT‑GATGAGGGAGAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑ACA‑TTCAAAAGGTTG‑ACAGAGATTGCCGCAAGG‑TTTACCGCCATG‑ATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACT‑aaca                                                                                                                                                <  2:831787/229‑3 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑ACA‑GAGAAACAGATT‑AAAAAAATTGTAGGTAAA‑TTTTAAGCCATG‑AGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACC‑aaac                                                                                                                                                >  2:958061/1‑238 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATTGAGTTTGCCT‑GATGAGAGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑ACA‑GAGAAAAAATTG‑ACAGAGATTGTTGCTAAA‑TTTAACGCCAAG‑ATTTTTACCCCAAAGCCCGATTACGGGGGGGATAAACT‑aaaa                                                                                                                                                >  1:459018/1‑239 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTAAGACAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑AAA‑ATGAAAAAGATG‑GCAGAGAGTGTTGCTAAC‑TATACCGCCCTG‑ATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACA‑aaaa                                                                                                                                                >  2:475605/1‑239 (MQ=255)

                                                                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTACGAAAAAAAC‑AAAAAATGGAGTTTTCAT‑TGTGGGAGGAAAGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAAAAAATTG‑ACAAAGATTGTGGCGTAC‑TTTTTCGCCCTG‑ATTTCTTTCCGAATACCTGATGACGTGGGGGGTATACA‑aaaaaa                                                                                                                                              >  2:582594/1‑237 (MQ=255)

                                                                                            gtcagaggtggataagaaacaggTTATTTACATATT‑CGCGGTGGAAAAAAATATAAAACCACATTTTAAGTGTTGGTGTGTGGCGGTGAATTTTACAAGACAAATAC‑AAAAACTGGAGTTTCCAT‑GATGAGCAAAATTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑TTA‑TTAAATAAGTTG‑ACAGAGATTGTCGTAAAT‑TTTACCGCCATG‑ATTTCTACCCTAATGCCTGATGACGTGGTGGATAAACT‑aaaa                                                                                                                                                <  2:660336/215‑1 (MQ=255)

                                                                                                 gctataatacaagaccttatAATTAAATGTT‑AAGGGTGGAAAAAAAGAAAAAACAACATTTTTAGGGGGAGTTTGTGGCGGTAATTTTTTCAAGACAAAACA‑AAAAACTGGGGTTGCCAT‑GAGGACGAAAAGTAATAACCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑TGGAAAAAGTTG‑ACAGAGTTTGTCTCTAAC‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTa                                                                                                                                          <  2:119960/219‑1 (MQ=255)

                                                                                                 gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCAGACAAAAAA‑AAAAACAGTAGGGTCCTG‑TATTGGGGGAAGTTATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAAATT‑GCAGAAATTGTCGCTAAC‑ATTACCCCCCAG‑AATTCTTCCCAAATGCCTGATTACGCGGTGGATAAATT‑aaaaagagtta                                                                                                                                         >  2:1151044/1‑232 (MQ=255)

                                                                                                 gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAAAGGAGTTGGCAT‑TATGAGGGGAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAGATG‑GCAGAGAATGTTGCTAAA‑TTTACCACCCTG‑ATTTCTACCCGAATACATGATGACTGGGGGGGTAAACA‑aaaaaaaataa                                                                                                                                         >  2:1102120/1‑232 (MQ=255)

                                                                                                     gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAATTTTTACAGACAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCG‑GTGAATAAGTTG‑ACAGAGTTTTTCACTAAC‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg‑g                                                                                                                                     <  2:1280570/231‑1 (MQ=255)

                                                                                                                 tGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATGGAGGTGTCAT‑GATGAGCGAAAGGAATAAATAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GGA‑GGGAATAAGATG‑ACAGAGATTTGCGCTAAC‑TTTAACCCCCAG‑ATTTCTACCCGCAAGCCTCATGACGTGGGGGAAAAAAT‑AAAAAAACTaaaaggcggccaaaagt                                                                                                                          >  1:697927/1‑224 (MQ=255)

                                                                                                                   ttAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAgaga                                                                                                                                                                                                                    <  1:697647/149‑1 (MQ=255)

                                                                                                                   taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                           <  1:263711/202‑1 (MQ=255)

                                                                                                                     aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTACCAGACAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGtcgtcg                                                                                                                    <  2:595367/239‑1 (MQ=255)

                                                                                                                       ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGAt                                                                                                                  <  1:1068744/239‑1 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAAAAAATTG‑AAAGAGAGTGTCGCTAAC‑TTTACCGCCATG‑ATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACT‑AAAAAACCTAAAG‑GATACCGAAAAGTCCTCGGTgggg                                                                                                              >  1:631210/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATTGAGTTTTCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAGTTG‑AAAGAGATTGTCGCTAAC‑TTTAACGCCCTG‑ATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAAAT‑AAAAAAGCTAAAG‑GATGACGCAACGCAGGCGATgggg                                                                                                              >  1:1388078/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTATAACAAATAA‑AAAAACAGGAGTTGGCAT‑TATGAGGGGAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑GGGGAAAAGTTT‑AGAGGGAATGTTGCTACA‑TTTATACCCATGAATTATTACCCAAAAGCC‑GGTGTTGTGGGGGATAAAAC‑AAAACAGCTAAAA‑AACTTCGGAAAGTCGTCGACgggg                                                                                                              >  2:1019021/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAATAC‑AAAAAAGGGGGGTGCCGT‑GGTGGGCTGGAGTAATAAAAAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GAA‑ATAACTAAGTTT‑TCTGACATTTGGGCTAAA‑TTTAAATCAATG‑CGTTCTTCCCCAACCCCTGAGTGCGCCGGGGGTAAAAA‑AAAACAGCTAAAGAGTTTCCTGAAC‑TCGTCGATgggg                                                                                                              >  2:229326/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTTAGACCAAAAA‑AAAAACTAGAGGTGGCAA‑GAAGAGGGAAAAAAATAAGCAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑GAGAATAAATTG‑TCCGAGAATGTGGCTAAA‑TTTACCTCCATC‑AATTTTTACCGAATGCCTGGTGTCGATGGGGATAAACT‑AAAACAACCAAAA‑Gaatgcgcaaacacgtacacgggg                                                                                                              >  2:794496/1‑217 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGa                                                                                                                              <  1:1049051/223‑1 (MQ=255)

                                                                                                                           atatT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTa                                                                                                                                          <  1:829964/212‑1 (MQ=255)

                                                                                                                            tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAATTTTTACATACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTGG‑ACAGAGATTGTCGTTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:1403772/239‑1 (MQ=255)

                                                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACCAAATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAAAAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  2:1332086/239‑1 (MQ=255)

                                                                                                                            attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:1064968/237‑1 (MQ=255)

                                                                                                                             tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTA‑ACAGATATTGCCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  2:251319/233‑1 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGAAAAAAAA‑AAAAAATTGAGTTGGCCT‑GGTGGGTGGAAAAAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑ACA‑AGGGATAAATTG‑GAAGAGATTGTTGCTaa                                                                                                                                                                                                          >  2:697647/1‑149 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACCAAAAC‑AAAAAATGGGGTTTGTTT‑GGTGGGCGCAAGTAATAAACAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAATTG‑CAAGAGATTTTTGCTAACATTAACCACCA‑G‑ATTTTTACCCGAATGCCTGCTGATGTGGGGGATTAAAT‑AAAAAAGCTTAAG‑GCTACAGaaaactagtgggtggggga                                                                                                            >  2:748633/1‑223 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAAACAAAAAC‑AAAAACAGGAGGTTGCAT‑GGTGAGCGGAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑AAA‑AGGGATAAGATGTACA‑AGAATGTGGCTAAA‑TATACCCCCATA‑ATTTTTTACCGAAAGTATGTTGTAGGGGTGGATTAAAC‑AAAACAACTTAAA‑GAGGCCGCAACCGCGTCGGTggggga                                                                                                            >  2:91747/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGGGGTGCCAT‑GATGGGCGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAA‑TTTACCGCCCTG‑ATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACT‑AAAAACGCTAAAA‑GAAGCCGAAAAGTCGGTGATggggga                                                                                                            >  1:1374632/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAAATGAGTTTGCAT‑GGTGAGCGGAAAAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑AGGAATAAATTT‑ACAAAGAATGTCGCCAAC‑TTTACCGCCCTT‑ATTTTTAACCCAATGGCTGATGACGTGGGGGGTAAAAT‑AAAACAACAAAAA‑GATTCCGCAACATCGTCGATggggga                                                                                                            >  1:1232761/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGaa                                                                                                            <  1:1151052/239‑1 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAA‑AAAAAATGGAGATGCCAT‑GATGAGCGGAAGTAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAAAAAAATG‑AAAGAGATTGTCGGCAAA‑TTTACCCCCATG‑ATTTTTACCCCAATGCCTGATGAGGGGGGGGGTAAAAT‑AAAAAAGCTAAAG‑Gaaagtctcttttacacatatccgc                                                                                                             >  1:660336/1‑215 (MQ=255)

                                                                                                                             aattacgggTGGAAAAAAAAAAAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAAAAC‑AAAACCTGGAGTGGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑TTAAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:636384/232‑1 (MQ=255)

                                                                                                                                  aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAA‑AAAAAATTGAGTTGCCCT‑GATTGGGGGAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAATTG‑ACAGAGATTTTCGCTAAC‑TTTTCCGCCATG‑ATTTTTACCCGAATGCCTGATTACGTGGGGGGTAAAAT‑AAAAAAGATAAAG‑GATGCCGAAAAGTCGGCGATGGGGGAAAtt                                                                                                        >  1:1201523/1‑239 (MQ=255)

                                                                                                                                   cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAA‑AAAAAATTGAGGTGGCCT‑GATTGGGGGAAGTAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAAATT‑ACAGAGAATGTGGCTAAC‑TTTACCCCCAAG‑ATTTTTATCCCGATGGACGAAAACGGGGGGGGTAAAATAAAAACACTTAAAG‑AATCCGGAAACGTGTCCGTGGGGGAAAAta                                                                                                        >  2:487241/1‑238 (MQ=255)

                                                                                                                                      tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCAT‑GATGAGGGGAAATAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑GCAGAGAATGTCGCCAAC‑TTTACCGCCATG‑ATTTCTACCCGCATGCCTTATTACGTGGTGGAGAAAAT‑AAAACAACTAAAA‑GATGCCGAAACGtcgtcgcctgccccttatacac                                                                                                    >  2:1049051/1‑223 (MQ=255)

                                                                                                                                      tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACATCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                                     <  1:134290/238‑1 (MQ=255)

                                                                                                                                              aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTTCCAAAAAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCAAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  2:1201523/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAACCCACATTTTGAGTGGGAGTTGGGGGGGGTGAATTTTTCCAAAAAAAAAC‑AAAAAGGGGAGTGGCAAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCG‑GTAAATAAGTGG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  1:229326/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAATTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑TGGAATAAGTTG‑ACAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  1:40417/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAAACCACATTTGTAGTGGTAGTGTGTGGCGGTAATTTTTTCAAGAAAAATAC‑AAAAACGGGAGTTGCCAG‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAGCAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  2:1374632/239‑1 (MQ=255)

                                                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGATG‑ACAGAGATTGTCGCCAAC‑TTTACCGCCCTG‑ATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACT‑AAAACAGCTAAAA‑GATGCCCAAACGACGTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                                               >  1:957057/1‑238 (MQ=255)

                                                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAAATG‑ACAAAGATTGTCGCCAAC‑TTTTCCg                                                                                                                                                                                                 >  1:77967/1‑129 (MQ=255)

                                                                                                                                                                       ggTTTGGGGTGGAAAATTTTACAAAAAAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGGAATAAGTTG‑ACAGAGATTGCCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                  <  2:652970/238‑1 (MQ=255)

                                                                                                                                                                       aGTTTGTGGCGGTAAATTTTTCAAAAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGGCCGAAAATAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATGGTCGCTACC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                  <  1:1151044/239‑1 (MQ=255)

                                                                                                                                                                             tgGCGGTGAATTTTTCCAGACAAAAAC‑AAAAATTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGAc                                                             <  1:669602/238‑1 (MQ=255)

                                                                                                                                                                               gCGGTAAATTTTCCAAGACAAATAA‑AAAAACTGGAGTTGCCAA‑GATGAGCAAAAGTAATAAGCAAAGGGC‑AAGGG‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATGGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                  <  2:558935/238‑1 (MQ=255)

                                                                                                                                                                                   taaaTTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGA‑ACAGAGATTGTCGCTACC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                                       <  1:147067/236‑1 (MQ=255)

                                                                                                                                                                                         ttaccAAACAAAAAA‑AAAAACTGAGTTTCCCAG‑GAAGAGCAAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑CGA‑GGAAATAAGTTG‑AAAGAGTGTGTCGTAAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                <  2:1232761/236‑1 (MQ=255)

                                                                                                                                                                                                     tAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                    <  1:475605/239‑1 (MQ=255)

                                                                                                                                                                                                      taaaaaaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                    <  1:642776/236‑1 (MQ=255)

                                                                                                                                                                                                         aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGATTTTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                 <  1:795382/239‑1 (MQ=255)

                                                                                                                                                                                                           aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                               >  2:1250640/1‑239 (MQ=255)

                                                                                                                                                                                                            accgggAGTTGCAAT‑GATGAGGGAAAGAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGAAAAAGGTTG‑AAAGAGATTGCCGCTACC‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:59610/235‑1 (MQ=255)

                                                                                                                                                                                                            aatgtgAGTTGCCGT‑GAGGAGAGAAAGAAAAAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GGTAATAAGTGG‑ACAGAGAGTGGCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:212011/234‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAAAAAGTTG‑ACAGAGTTTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:1349641/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:615170/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:794496/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGTTGGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:905093/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAAT‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:582594/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGAAGTTGCCAT‑GATGAGCGAAATTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAATAAGTTG‑GCAGAGATCGTACCTAAT‑TTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:793625/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                             >  2:931448/1‑239 (MQ=255)

                                                                                                                                                                                                                 gAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑CATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCACAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGCACCCGCGGAGAtt                         >  2:570219/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg         >  1:545939/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                   <  1:1112121/197‑1 (MQ=255)

                                                                                                                                                                                                                                     aaGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg      >  1:909508/1‑239 (MQ=255)

                                                                                                                                                                                                                                         aaaaaGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAAAAATTAG‑AAAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc  <  1:355740/236‑1 (MQ=255)

                                                                                                                                                                                                                                          ataaGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc  >  2:137945/1‑238 (MQ=255)

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAC‑TTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC  >  NC_000913/3206247‑3206714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`