Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,493 G→A 13.5% V11V (GTG→GTA) ‡ ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4930GA13.5% 241.7 / 9.6 120V11V (GTG→GTA) ‡ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (50/51);  new base A (2/15);  total (52/68)
Fisher's exact test for biased strand distribution p-value = 3.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTT  >  NC_000913/3206255‑3206730
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTg                                                                                                                                                                                                                                                   <  1:1290429/239‑1 (MQ=255)
         tCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTg                                                                                                                                                                                                                                          <  1:1083770/239‑1 (MQ=255)
                        aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAAC‑AA‑‑‑‑‑‑GCA‑GGGAAAAAAGTGACAGAGATGTTCGc                                                                                                                                                                                                                            >  2:894581/1‑238 (MQ=255)
                        aGCGAAGGGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCGGGCGCAATATTCATTCTTATTTCCGCGTTGATTCACGAACTGTTAATTACTTATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                           <  2:800534/239‑1 (MQ=255)
                          cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAAAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAAA‑AA‑‑‑‑‑‑GCACGTAACTAA‑TTGGCAGAGAtttagcgat                                                                                                                                                                                                                          >  2:147067/1‑231 (MQ=255)
                          cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTACAAATTC‑AAAAAATTGAGTTTCACT‑GATGAGCGCAAATAAAAAACACA‑AG‑‑‑‑‑‑GCA‑GGGAATAAAATTACATAGATTGTTTCTaa                                                                                                                                                                                                                         >  1:800534/1‑239 (MQ=255)
                               tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                                                                                                                                                                                    <  1:1082447/239‑1 (MQ=255)
                                      cGCAAAACATTCCCA‑TGCCTTGCTTCGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCAAACTTTTAATTACATATA‑CTCGGTGGCAAAAAATAAAAACCAAAATTTTTGGTGGTAGTTTGGGGAGGTGAATTTTTCCAGAAAAAAAC‑AAAAACTGAGGTTGCAAT‑GATGAGCGAAAGTAAAAAGCACC‑AG‑‑‑‑‑‑GAA‑GTGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAt                                                                                                                                                                                                             <  1:618457/239‑1 (MQ=255)
                                         aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTAACAGAGTTTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                                         <  2:1306196/233‑1 (MQ=255)
                                         aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAAAGAATGTCGCTAACTTTAACGCCATG‑At                                                                                                                                                                                                         >  1:251319/1‑239 (MQ=255)
                                         aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAAAGGAGGTGCCAT‑GATGAGCGGAAGAAATAAGCAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAGTTTACAGAGATTGTGGCTAAATTTATCCCCCTG‑At                                                                                                                                                                                                         >  1:59610/1‑239 (MQ=255)
                                           aaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑Attt                                                                                                                                                                                                       <  1:1192224/239‑1 (MQ=255)
                                                tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAAAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAAA‑AA‑‑‑‑‑‑GCA‑GGGGAAAAAATTAAAGAGATTGGTGCTCACTTTTACGCCATG‑Aatttttc                                                                                                                                                                                                   >  1:642912/1‑234 (MQ=255)
                                                  ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAACAAGCAAAAAGCA‑GCGGATAAGTTGACAGAGATTTTTGCTAAATTTACCCACATG‑Attt                                                                                                                                                                                                       >  1:558935/1‑239 (MQ=255)
                                                    ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GCGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                                                               <  1:677346/237‑1 (MQ=255)
                                                    a‑TTCCCTGCTCTGGCACAATATTCTTTCTTTTTTTCGCGTTGATTCACGAACGGTTAATTACATTTT‑CACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                                              <  1:482289/239‑1 (MQ=255)
                                                        cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAAA‑AA‑‑‑‑‑‑GCA‑ACGAATAAGATTAGAGAGAATGTGGGTCACTTTACCGCCCTG‑ATTTCTATCCGAATGc                                                                                                                                                                                           >  2:970454/1‑239 (MQ=255)
                                                                 ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATCGAGTTTTCCT‑GATGTGGGAAAGTAATAAACAAC‑AA‑‑‑‑‑‑GCA‑ATGAATAAATTTACAGAGAATGTCGCTAAATTTAACTCCATG‑TTTTTTACCCGCATGACcgatgtgg                                                                                                                                                                                  >  1:623602/1‑236 (MQ=255)
                                                                  gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAAC‑AC‑‑‑‑‑‑GCA‑CAGAATAAGTTTTCAGAGATTGTCGTTACCTTTATAGCCCCG‑CTTTCTATCCGAATGccccgggacgt                                                                                                                                                                                 >  2:968220/1‑230 (MQ=255)
                                                                    gcAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATT‑CAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAAACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                                <  1:970454/238‑1 (MQ=255)
                                                                        tatTCTATCTTATTTTCGAGTTTATTCACGAACTGTTAATTAAATATC‑CAGGGTGCCAAAAAATATAAAACCTCATTTGTAGGGGAAGTTTGTGGGGGTGAATTTTCCAAGACAAATAC‑AAAACCTGGAGTTGCCAT‑GAGTAGCAAAAATAAAAAGCACC‑AG‑‑‑‑‑‑GCA‑GTGAATAATTTGACAGAATTTGCCGCTATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                           <  2:1286999/239‑1 (MQ=255)
                                                                        aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAATTGTTCAAAAAAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAGA‑AG‑‑‑‑‑‑ACA‑TGAAATAAGTGGAAGGGAATTGGCGCAAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                           <  2:1291506/234‑1 (MQ=255)
                                                                          ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGGGTTTCCAT‑GATGAGCGAGAGAAATAAGCAAC‑AA‑‑‑‑‑‑GCA‑GTGAATAAATTTACAGAGATTGTCGCTAAATTTACCGCCATG‑ATTTCTACCCCCATGCCTGTTGGCGGGGGGGCTa                                                                                                                                                                         >  1:1245946/1‑239 (MQ=255)
                                                                                cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                                                  <  1:328413/239‑1 (MQ=255)
                                                                                 ttATTTTCGCGTTGATTCACGAACTGTTAATTACATTGT‑CACGGTGGAAAAAAATAGAAACCCACATTTGTAGTGGTATTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGACTGATGACGTGGTGGATAAACT‑aaa                                                                                                                                                                 <  1:359612/239‑1 (MQ=255)
                                                                                  tatttactgtttGATTCCCTAACTGTTAATTACATATT‑CGAGGTGGAAAAAAATAGAAAACCATGTTTGTATTGGTGGTTTGGGGCGGAAAATTGTTCCTGAAAAAGAC‑AAAATGTGGACGTTCACT‑GATGAGGGAGAGTAATAAGCAAC‑GG‑‑‑‑‑‑ACA‑TTCAAAAGGTTGACAGAGATTGCCGCAAGGTTTACCGCCATG‑ATTTCTTCCCGAATGCCTGATGACGTGGTGGATAAACT‑aaca                                                                                                                                                                <  2:831787/229‑3 (MQ=255)
                                                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAAC‑AG‑‑‑‑‑‑ACA‑GAGAAACAGATTAAAAAAATTGTAGGTAAATTTTAAGCCATG‑AGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACC‑aaac                                                                                                                                                                >  2:958061/1‑238 (MQ=255)
                                                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATTGAGTTTGCCT‑GATGAGAGAAAGTAATAAACAAC‑AA‑‑‑‑‑‑ACA‑GAGAAAAAATTGACAGAGATTGTTGCTAAATTTAACGCCAAG‑ATTTTTACCCCAAAGCCCGATTACGGGGGGGATAAACT‑aaaa                                                                                                                                                                >  1:459018/1‑239 (MQ=255)
                                                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTAAGACAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAAA‑AG‑‑‑‑‑‑AAA‑ATGAAAAAGATGGCAGAGAGTGTTGCTAACTATACCGCCCTG‑ATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACA‑aaaa                                                                                                                                                                >  2:475605/1‑239 (MQ=255)
                                                                                    ttttCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTACGAAAAAAAC‑AAAAAATGGAGTTTTCAT‑TGTGGGAGGAAAGAAAAAACAAA‑AA‑‑‑‑‑‑GCA‑GAGAAAAAATTGACAAAGATTGTGGCGTACTTTTTCGCCCTG‑ATTTCTTTCCGAATACCTGATGACGTGGGGGGTATACA‑aaaaaa                                                                                                                                                              >  2:582594/1‑237 (MQ=255)
                                                                                    gtcagaggtggataagaaacaggTTATTTACATATT‑CGCGGTGGAAAAAAATATAAAACCACATTTTAAGTGTTGGTGTGTGGCGGTGAATTTTACAAGACAAATAC‑AAAAACTGGAGTTTCCAT‑GATGAGCAAAATTAATAAGCAAC‑AG‑‑‑‑‑‑TTA‑TTAAATAAGTTGACAGAGATTGTCGTAAATTTTACCGCCATG‑ATTTCTACCCTAATGCCTGATGACGTGGTGGATAAACT‑aaaa                                                                                                                                                                <  2:660336/215‑1 (MQ=255)
                                                                                         gctataatacaagaccttatAATTAAATGTT‑AAGGGTGGAAAAAAAGAAAAAACAACATTTTTAGGGGGAGTTTGTGGCGGTAATTTTTTCAAGACAAAACA‑AAAAACTGGGGTTGCCAT‑GAGGACGAAAAGTAATAACCAAC‑AG‑‑‑‑‑‑GCA‑TGGAAAAAGTTGACAGAGTTTGTCTCTAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTa                                                                                                                                                          <  2:119960/219‑1 (MQ=255)
                                                                                         gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCAGACAAAAAA‑AAAAACAGTAGGGTCCTG‑TATTGGGGGAAGTTATAAGCAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAAATTGCAGAAATTGTCGCTAACATTACCCCCCAG‑AATTCTTCCCAAATGCCTGATTACGCGGTGGATAAATT‑aaaaagagtta                                                                                                                                                         >  2:1151044/1‑232 (MQ=255)
                                                                                         gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAAAGGAGTTGGCAT‑TATGAGGGGAAGTAATAAGAAAC‑AA‑‑‑‑‑‑GCA‑ATGAATAAGATGGCAGAGAATGTTGCTAAATTTACCACCCTG‑ATTTCTACCCGAATACATGATGACTGGGGGGGTAAACA‑aaaaaaaataa                                                                                                                                                         >  2:1102120/1‑232 (MQ=255)
                                                                                           gtttataagagacgggTTAATTACAGATT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGACAAATAC‑AAAACCTGGAGTGGCAAT‑GATGAGGAAAAGTAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGCAAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg                                                                                                                                                       <  1:1826/224‑1 (MQ=255)
                                                                                             gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAATTTTTACAGACAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCG‑GTGAATAAGTTGACAGAGTTTTTCACTAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg‑g                                                                                                                                                     <  2:1280570/231‑1 (MQ=255)
                                                                                                         tGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATGGAGGTGTCAT‑GATGAGCGAAAGGAATAAATAAC‑AA‑‑‑‑‑‑GGA‑GGGAATAAGATGACAGAGATTTGCGCTAACTTTAACCCCCAG‑ATTTCTACCCGCAAGCCTCATGACGTGGGGGAAAAAAT‑AAAAAAACTaaaaggcggccaaaagt                                                                                                                                          >  1:697927/1‑224 (MQ=255)
                                                                                                          gTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCCT‑GATGAGGGAAAGTAATAAGCAAA‑AA‑‑‑‑‑‑GCA‑GAGAATAAGTTTACAGAGAATTTTGCTAACTTTAACCCCATG‑ATTTTTACCCGAAAGCCTTAATAAGTGGGGGGTAAACT‑AAAAAAGATAAAA‑GAAGGCGAAAcagcg                                                                                                                                       >  1:652970/1‑235 (MQ=255)
                                                                                                           ttAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAgaga                                                                                                                                                                                                                                   <  1:697647/149‑1 (MQ=255)
                                                                                                           taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                           <  1:263711/202‑1 (MQ=255)
                                                                                                             aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTACCAGACAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGtcgtcg                                                                                                                                    <  2:595367/239‑1 (MQ=255)
                                                                                                               ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAAC‑AG‑‑‑‑‑‑GGA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGAt                                                                                                                                  <  1:1068744/239‑1 (MQ=255)
                                                                                                                   atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAAA‑AA‑‑‑‑‑‑GCA‑GTGAAAAAATTGAAAGAGAGTGTCGCTAACTTTACCGCCATG‑ATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACT‑AAAAAACCTAAAG‑GATACCGAAAAGTCCTCGGTgggg                                                                                                                              >  1:631210/1‑239 (MQ=255)
                                                                                                                   atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATTGAGTTTTCAT‑GATGAGCGAAAGTAATAAGAAAC‑AG‑‑‑‑‑‑GCA‑GGGAATAAGTTGAAAGAGATTGTCGCTAACTTTAACGCCCTG‑ATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAAAT‑AAAAAAGCTAAAG‑GATGACGCAACGCAGGCGATgggg                                                                                                                              >  1:1388078/1‑239 (MQ=255)
                                                                                                                   atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTATAACAAATAA‑AAAAACAGGAGTTGGCAT‑TATGAGGGGAAAAAATAAGAAAC‑AA‑‑‑‑‑‑GAA‑GGGGAAAAGTTTAGAGGGAATGTTGCTACATTTATACCCATGAATTATTACCCAAAAGCC‑GGTGTTGTGGGGGATAAAAC‑AAAACAGCTAAAA‑AACTTCGGAAAGTCGTCGACgggg                                                                                                                              >  2:1019021/1‑239 (MQ=255)
                                                                                                                   atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAATAC‑AAAAAAGGGGGGTGCCGT‑GGTGGGCTGGAGTAATAAAAAAC‑AC‑‑‑‑‑‑GAA‑ATAACTAAGTTTTCTGACATTTGGGCTAAATTTAAATCAATG‑CGTTCTTCCCCAACCCCTGAGTGCGCCGGGGGTAAAAA‑AAAACAGCTAAAGAGTTTCCTGAAC‑TCGTCGATgggg                                                                                                                              >  2:229326/1‑239 (MQ=255)
                                                                                                                   atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTTAGACCAAAAA‑AAAAACTAGAGGTGGCAA‑GAAGAGGGAAAAAAATAAGCAAC‑AA‑‑‑‑‑‑GAA‑GAGAATAAATTGTCCGAGAATGTGGCTAAATTTACCTCCATC‑AATTTTTACCGAATGCCTGGTGTCGATGGGGATAAACT‑AAAACAACCAAAA‑Gaatgcgcaaacacgtacacgggg                                                                                                                              >  2:794496/1‑217 (MQ=255)
                                                                                                                   atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGa                                                                                                                                              <  1:1049051/223‑1 (MQ=255)
                                                                                                                    tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAATTTTTACATACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTGGACAGAGATTGTCGTTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                                             <  1:1403772/239‑1 (MQ=255)
                                                                                                                    tatT‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGAAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                                             <  1:469404/239‑1 (MQ=255)
                                                                                                                    tatT‑CACGGTGGAAAAAAATATAAAACCAAATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAAAAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                                             <  2:1332086/239‑1 (MQ=255)
                                                                                                                    attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAAC‑GG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                                             <  1:1064968/237‑1 (MQ=255)
                                                                                                                     tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTAACAGATATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGa                                                                                                                             <  2:251319/233‑1 (MQ=255)
                                                                                                                     atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGAAAAAAAA‑AAAAAATTGAGTTGGCCT‑GGTGGGTGGAAAAAATAAACAAA‑AA‑‑‑‑‑‑ACA‑AGGGATAAATTGGAAGAGATTGTTGCTaa                                                                                                                                                                                                                         >  2:697647/1‑149 (MQ=255)
                                                                                                                     atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGGGGTGCCAT‑GATGGGCGAAAGTAATAAAAAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAAATTTACCGCCCTG‑ATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACT‑AAAAACGCTAAAA‑GAAGCCGAAAAGTCGGTGATggggga                                                                                                                            >  1:1374632/1‑237 (MQ=255)
                                                                                                                     atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAAATGAGTTTGCAT‑GGTGAGCGGAAAAAATAAACAAC‑AA‑‑‑‑‑‑GCA‑AGGAATAAATTTACAAAGAATGTCGCCAACTTTACCGCCCTT‑ATTTTTAACCCAATGGCTGATGACGTGGGGGGTAAAAT‑AAAACAACAAAAA‑GATTCCGCAACATCGTCGATggggga                                                                                                                            >  1:1232761/1‑237 (MQ=255)
                                                                                                                     atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGaa                                                                                                                            <  1:1151052/239‑1 (MQ=255)
                                                                                                                     atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAA‑AAAAAATGGAGATGCCAT‑GATGAGCGGAAGTAATAAGAAAA‑AA‑‑‑‑‑‑GCA‑ATGAAAAAAATGAAAGAGATTGTCGGCAAATTTACCCCCATG‑ATTTTTACCCCAATGCCTGATGAGGGGGGGGGTAAAAT‑AAAAAAGCTAAAG‑Gaaagtctcttttacacatatccgc                                                                                                                             >  1:660336/1‑215 (MQ=255)
                                                                                                                          aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAA‑AAAAAATTGAGTTGCCCT‑GATTGGGGGAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAATTGACAGAGATTTTCGCTAACTTTTCCGCCATG‑ATTTTTACCCGAATGCCTGATTACGTGGGGGGTAAAAT‑AAAAAAGATAAAG‑GATGCCGAAAAGTCGGCGATGGGGGAAAtt                                                                                                                        >  1:1201523/1‑239 (MQ=255)
                                                                                                                           cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAA‑AAAAAATTGAGGTGGCCT‑GATTGGGGGAAGTAAAAAACAAA‑AA‑‑‑‑‑‑GCA‑ATGAATAAAATTACAGAGAATGTGGCTAACTTTACCCCCAAG‑ATTTTTATCCCGATGGACGAAAACGGGGGGGGTAAAATAAAAACACTTAAAG‑AATCCGGAAACGTGTCCGTGGGGGAAAAta                                                                                                                        >  2:487241/1‑238 (MQ=255)
                                                                                                                            gagaaaaaaaaaTA‑AAAACCAAATTTTTGTGGGTGGTTTGTGAGGGTGAATTTTTCAAAAAAAATAA‑AAAAACGGGAGTTGCCTG‑GATGAGAAAAATAAAAAAACAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAGGTTGACAGAGATTGCCGATAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                      <  2:459018/232‑1 (MQ=255)
                                                                                                                             ggggCAAAAAAAAAAAAACCAAATGTTTAGTGGTAGTTTGGGGCGGTGAATATTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAATAAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAATTTGACAGAGATTGTCGCAACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAAGTAt                                                                                                                      <  2:576654/236‑1 (MQ=255)
                                                                                                                             gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                      <  1:958061/238‑1 (MQ=255)
                                                                                                                             gTGGAAAAAAAAAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAA‑AAAACCTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                      <  2:623602/238‑1 (MQ=255)
                                                                                                                              tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCAT‑GATGAGGGGAAATAATAAACAAC‑AG‑‑‑‑‑‑GCA‑GTGAAAAAGTTGGCAGAGAATGTCGCCAACTTTACCGCCATG‑ATTTCTACCCGCATGCCTTATTACGTGGTGGAGAAAAT‑AAAACAACTAAAA‑GATGCCGAAACGtcgtcgcctgccccttatacac                                                                                                                    >  2:1049051/1‑223 (MQ=255)
                                                                                                                              tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACATCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                                                     <  1:134290/238‑1 (MQ=255)
                                                                                                                                      aaTATAAAACCACTTTTTTAGTGGTAGTGTGTGGCGGTGAATTTTTCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGTAATAAGCACC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGCCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                           <  1:748633/239‑1 (MQ=255)
                                                                                                                                      aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTTCCAAAAAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTGACAGAGATTGTCGCAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                           <  2:1201523/239‑1 (MQ=255)
                                                                                                                                      aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAATTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GAA‑TGGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                           <  1:40417/239‑1 (MQ=255)
                                                                                                                                      aaTAAAAAACCACATTTGTAGTGGTAGTGTGTGGCGGTAATTTTTTCAAGAAAAATAC‑AAAAACGGGAGTTGCCAG‑GAGGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAATTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAGCAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                                           <  2:1374632/239‑1 (MQ=255)
                                                                                                                                        tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCt                                                                                                                                                                                                                           <  2:77967/129‑1 (MQ=255)
                                                                                                                                                  caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAAC‑AA‑‑‑‑‑‑GCA‑GTGAATAAGATGACAGAGATTGTCGCCAACTTTACCGCCCTG‑ATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACT‑AAAACAGCTAAAA‑GATGCCCAAACGACGTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                                                               >  1:957057/1‑238 (MQ=255)
                                                                                                                                                  caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAAC‑AG‑‑‑‑‑‑GGA‑GTGAATAAAATGACAAAGATTGTCGCCAACTTTTCCg                                                                                                                                                                                                                 >  1:77967/1‑129 (MQ=255)
                                                                                                                                                               ggTTTGGGGTGGAAAATTTTACAAAAAAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAAC‑AG‑‑‑‑‑‑GAA‑GGGAATAAGTTGACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                                  <  2:652970/238‑1 (MQ=255)
                                                                                                                                                               aGTTTGTGGCGGTAAATTTTTCAAAAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGGCCGAAAATAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTGACAGAGATGGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                                  <  1:1151044/239‑1 (MQ=255)
                                                                                                                                                                           taaaTTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTGAACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                                                       <  1:147067/236‑1 (MQ=255)
                                                                                                                                                                                 ttaccAAACAAAAAA‑AAAAACTGAGTTTCCCAG‑GAAGAGCAAAAGTAATAAACAAC‑AG‑‑‑‑‑‑CGA‑GGAAATAAGTTGAAAGAGTGTGTCGTAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                <  2:1232761/236‑1 (MQ=255)
                                                                                                                                                                                              taaaaaaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GAA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                                    <  1:642776/236‑1 (MQ=255)
                                                                                                                                                                                                 aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAAC‑GG‑‑‑‑‑‑GCA‑GTGAATAAGTGGACAGATTTTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                                 <  1:795382/239‑1 (MQ=255)
                                                                                                                                                                                                   aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                               >  2:1250640/1‑239 (MQ=255)
                                                                                                                                                                                                    accgggAGTTGCAAT‑GATGAGGGAAAGAAAAAAGCAAC‑AG‑‑‑‑‑‑GAA‑GGAAAAAGGTTGAAAGAGATTGCCGCTACCTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                              <  2:59610/235‑1 (MQ=255)
                                                                                                                                                                                                    aatgtgAGTTGCCGT‑GAGGAGAGAAAGAAAAAAGCAAC‑GG‑‑‑‑‑‑GCA‑GGTAATAAGTGGACAGAGAGTGGCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                              <  2:212011/234‑1 (MQ=255)
                                                                                                                                                                                                    aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAAC‑AG‑‑‑‑‑‑GAA‑GTAAAAAAGTTGACAGAGTTTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                              <  2:1349641/239‑1 (MQ=255)
                                                                                                                                                                                                    aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑GG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                              <  1:615170/239‑1 (MQ=255)
                                                                                                                                                                                                    aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                              <  1:794496/239‑1 (MQ=255)
                                                                                                                                                                                                    aaCTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGAAAGAGTTGGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                              <  1:905093/239‑1 (MQ=255)
                                                                                                                                                                                                     aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                             >  2:931448/1‑239 (MQ=255)
                                                                                                                                                                                                         gAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑CATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCACAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGCACCCGCGGAGAtt                                         >  2:570219/1‑239 (MQ=255)
                                                                                                                                                                                                                          cGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                         >  1:545939/1‑239 (MQ=255)
                                                                                                                                                                                                                          cGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                         >  1:561009/1‑239 (MQ=255)
                                                                                                                                                                                                                             aaGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg                      >  1:909508/1‑239 (MQ=255)
                                                                                                                                                                                                                                 aaaaaGCAAC‑AG‑‑‑‑‑‑GCA‑GGGAAAAATTAGAAAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                  <  1:355740/236‑1 (MQ=255)
                                                                                                                                                                                                                                  ataaGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                  >  2:137945/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GCTGCCGAAAAGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACACCATGCAAAAAGCGATTGACCTGAATCATCCTGCCTGTCAGGACGCCGGGGGAATTATGTTCTTCGTTAAAGTCGGATCCCGCt             >  1:798199/1‑238 (MQ=255)
                                                                                                                                                                                                                                          c‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc          >  2:1054292/1‑238 (MQ=255)
                                                                                                                                                                                                                                          c‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc          >  2:410470/1‑238 (MQ=255)
                                                                                                                                                                                                                                          c‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc          >  1:910312/1‑238 (MQ=255)
                                                                                                                                                                                                                                                     cA‑GTGAATAAGTTGACAGAGATTGGCGCAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc         <  1:319521/235‑1 (MQ=255)
                                                                                                                                                                                                                                                         tGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCCCTGCtt  >  1:1181337/1‑239 (MQ=255)
                                                                                                                                                                                                                                                         agAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGATCCCGCTTCCCACTGCtt  >  2:174114/2‑239 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 
CGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTT  >  NC_000913/3206255‑3206730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑