BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,760,368	T→C	16.6%	D294G (GAC→GGC)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,368	0	T	C	16.6%	230.8 / 5.3	120	D294G (GAC→GGC)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand): ref base T (60/30); new base C (2/21); total (63/57)
Fisher's exact test for biased strand distribution p-value = 4.92e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CTGTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTC  >  NC_000913/3760133‑3760604

cTGTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGc                                                                                                                                                                                                                                             <  2:827188/239‑1 (MQ=255)

          tgtgCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATTTCTTTTTTACCAAC‑AATAATTTCTGCCTGCGG‑CCCGCCTAAAC‑ACTTG‑TCGCCGGAGAAA‑c                                                                                                                                                                                                                                   >  1:979089/1‑238 (MQ=255)

          tgtgCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑GCCCCGGGGaaaa                                                                                                                                                                                                                                    >  1:104617/1‑237 (MQ=255)

                 ggATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATT‑CTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCg                                                                                                                                                                                                                                              <  1:256089/220‑1 (MQ=255)

                 ggATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATT‑CTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCg                                                                                                                                                                                                                                              >  2:256089/1‑220 (MQ=255)

                  gATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACCCAC‑AATAATTCCTGCCTGCGG‑CCCGCCTTACA‑ACTTT‑TCGCCGGAGAAA‑ACCACCAgg                                                                                                                                                                                                                           >  2:281663/1‑237 (MQ=255)

                            aCCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑AATTT‑TCGCCGGAGAAA‑CTCACCAGACTGAcgcccg                                                                                                                                                                                                                 >  1:513746/1‑238 (MQ=255)

                              ctctGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGAcgcccgcc                                                                                                                                                                                                               >  1:228301/1‑238 (MQ=255)

                              cgctgcgcggggGTAAAGCGGGGGCGGGGGCGGTATTTTTCCCCTCAGGGCTTCAGGGTGT‑AAAAAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGGGGGTC‑ATTTTACCCGCGCGTAATGCCCGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AAAAATTCCTGCCTGCGG‑CCCGCCAAACA‑CCTGG‑CCGCCGGAAAAC‑CCCCCCAGACTGACGCccgccg                                                                                                                                                                                                              <  2:952136/237‑1 (MQ=255)

                                    gcggcgGGTAAGCAGGGGGAGGGTCGGTAATTTTCCACGCAGAGCTTCAGGGTGT‑AAATAAAGACGCACCGGG‑GCTTCC‑AGGGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGGGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTCCCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑CCTTG‑TCGCCGGAAAAA‑CTCACCAGACTGCCGCCCGCCGCAATc                                                                                                                                                                                                         <  2:1154812/235‑1 (MQ=255)

                                        cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTTTTTTTTACCCAC‑CATAATTCCTGCCTTCGG‑CCCGCCTAACC‑ACTTT‑CCGCCGGCGAAA‑CTCACCACACTGACCCCCGCCCCACTCAAcc                                                                                                                                                                                                     >  2:1296970/1‑237 (MQ=255)

                                        cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCCATCAACt                                                                                                                                                                                                     >  1:114565/1‑238 (MQ=255)

                                           gTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTAACAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCt                                                                                                                                                                                                  >  1:833361/1‑238 (MQ=255)

                                                    cgcAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTTCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCCCCGGAGAAA‑CTCACCAGACTGAAGCCCGCCGCAATCCAATCCCTCGGGATTg                                                                                                                                                                                         >  2:1070077/1‑238 (MQ=255)

                                                      cAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGc                                                                                                                                                                                       >  1:929781/1‑238 (MQ=255)

                                                           tCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTTTTTTTTAACAAC‑AATAATTCCTGCCTGCCG‑CCCGCCTAACA‑ACTTT‑TTGCCGGCGAAA‑CTCACCAGACTGACGCCCGCCGCAATCCACTCCTGCGGCACTTGCTCTTTc                                                                                                                                                                                 >  2:1354730/1‑239 (MQ=255)

                                                             gatAATTTTCCACTCAGAGC‑TAAGGGTTTAAAAGAAGGCCGAAACGGG‑GATTCC‑GACGCCGGGGGTTTC‑TTTTTACCCGCGCATAATGCACGTTCCAGCGGGTGGCTTTGGGGGCGGGCGTTCATCTCTTTTTTCCCAAC‑AAAAATTCCGGCCTGCGG‑CCCGCCTAACA‑CCTTG‑CCGCCGGAAAAC‑CCCACCACACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCg                                                                                                                                                                                <  2:251563/236‑1 (MQ=255)

                                                                 aTTTTTCCCGCAGAGCTTCAGGGTGA‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGGCGGGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                                                                                           <  1:59636/239‑1 (MQ=255)

                                                                 aTTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTGCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATCCCTGCCTGCGG‑CCCGCCAAACA‑ACTTG‑TCGCCGGAAAAC‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                                                                                           <  1:373867/239‑1 (MQ=255)

                                                                  ttttccccccAGAGCTCACGGGTGA‑AAAAAAAGACGCAGCGTG‑GCTCCC‑AGCCCCGGGGGGGTCTTTTTTACCCGCGCGTAATGCACGCTCCAGCGGGTGCTTTTGCAGGCGGGCAATCATCTCTTTTTTCCCAAC‑AATATTTCCTGCCTGCGG‑CCCGCC‑AACACCCTTG‑TCGCCGGAAAAC‑CCCACCAGACTACCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCaaa                                                                                                                                                                           <  2:330937/230‑1 (MQ=255)

                                                                      tCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                      <  1:196752/239‑1 (MQ=255)

                                                                      gcACTAAGAGCGTCAGGGTGT‑AAAT‑AAGGCGC‑CCGTGTGCGTCCGTGCGCCGCTGGGGTC‑ATTTTACCCGCGCATATCGCTCCCTTCAGGGGGTGGTTTTGCGGGCGGGCTACCATCGCTTTTTT‑CCAACAAATAATTCCGTCCGGCGA‑CCCGCCAAACA‑ACTGG‑GCGCGGAAGAAA‑CTCACAAGACTAACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                      <  2:1002115/238‑1 (MQ=255)

                                                                      cgcccaagagCTTCAGGTGGT‑AAAAAAAGCGGCAAGGGG‑GCTTGC‑GGCGCGGGGCGGGTC‑TTTTAATCCTGGCGTAATGCCCGCTCCAGCGGGTGGCTTTGAGGGCGGGCGACCACCTTTTTTTTCCCAAA‑AATAATTCCGGCCTGGGG‑CCCGCCTAACA‑ACTTG‑TCGCGGGAGAAA‑CCCCCCAGACTGACGCCCGACGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                      <  2:1380956/233‑1 (MQ=255)

                                                                      ccacacaGAGTTTCAGGGTGA‑AAATAAAGCCGAACCGGG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTACCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGAGGGCGGGCGATCATCTCTTTTTTACCAAA‑AATAATCCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑CCGCCGGAGAAA‑CTCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                      <  2:621189/238‑1 (MQ=255)

                                                                      ccACTCATTGCTTGAGGGTTT‑AAATAAAGCCGCACTGTG‑CCTTCC‑AGCGCCGGGGGGGTC‑ATTTTTGCCGCGCGTAATCCCGGATTCGGCGGGTGGTTTTGCGGGCGGGCGATCATCTCTTTTTTCCCAAC‑AATATTCCCTGCCTGCGG‑CCCCCCTAACA‑ATTTT‑TCGCGGGAAAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                      <  2:500980/238‑1 (MQ=255)

                                                                          gcAGAGCTGCATGGTGT‑AAATAAAGTCGCAGCGGG‑GCTTCC‑CGCGCCGCGAGTGTC‑ATTGTATCCTCGCGTACTGCACGCCTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATATTCCCTGCATGCGC‑CCCGCCTAACA‑CCTTG‑GCGCCGGAAAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTgg                                                                                                                                                                  <  2:1072368/238‑1 (MQ=255)

                                                                           cAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GTTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTCCCAAA‑AATAATCCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGc                                                                                                                                                                 <  1:1435781/239‑1 (MQ=255)

                                                                                 aaaaGGGTGA‑AAAAAAAGCCGCACCGTG‑CGTCCC‑CGCGCCGGGAGGCTC‑TTTTTACCCGCGCGTAATGCACCCTTCGGGGGGTGCTTTTGCGGGCGGGCGATCATCTCTTTTTACCCAAA‑AATAATCCCGGCCGGCGC‑CCCCCTTAAAA‑ACTTG‑CCGCGGGAGTAA‑CCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAg                                                                                                                                                             <  2:516524/234‑1 (MQ=255)

                                                                                        tgt‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCa                                                                                                                                                     >  1:655659/1‑238 (MQ=255)

                                                                                          t‑aaataaaGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAAAA‑ACTTG‑TCGCCGGAGAAA‑CTCACCCGACTGACGCCCGCCGCAATCAACTCCCGCGGCCCTGGCTCTTTCGGGAAACCGGACTGGCTAAGATCGACCAGCg                                                                                                                                                  >  2:439620/1‑239 (MQ=255)

                                                                                                aaaGCCACAGCGTG‑GCTCCC‑AGCGCCGGGAGGGTC‑TTTTTACCCGCGCGTAATGCACGCTTCAGCGGGGGGCTTTGCGGGCGGGCGATCTTCTCTTTTTTACCAAC‑AATAATCCCTGCCGGCGC‑CCCGCCTAACA‑TCTTG‑TCGCCGGAGAAA‑CTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                             <  2:181686/239‑1 (MQ=255)

                                                                                                aaaGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑CCGCCGGAGAAC‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                             <  1:724728/239‑1 (MQ=255)

                                                                                                 aaGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGcc                                                                                                                                              >  1:53140/1‑237 (MQ=255)

                                                                                                   gACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTTCCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCCTTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACt                                                                                                                                           >  1:1015312/1‑238 (MQ=255)

                                                                                                   gACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCCAC‑AATAATTCCTGCCTGCGG‑CCCGCCCAACA‑ACTTG‑TCGCCGGAGTAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAAACGTACTGGCTAAGATCGACCAGc                                                                                                                                                   >  1:516524/1‑230 (MQ=255)

                                                                                                     cGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCCGACTGACGCCCGCCCCAATCAACTCCTGCGGCATTGGCTCTTTTGGCAAACCGTACTGGCTAAGATCCACCCGCGacacccttc                                                                                                                                         >  2:198797/1‑230 (MQ=255)

                                                                                                     cGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTg                                                                                                                                          >  1:812378/1‑237 (MQ=255)

                                                                                                     cGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑ATCACCAGACCGACGCCCGCCCCAATCCACTCCTGCCGCATTGGCCCTTTCGGCCAACCCGACTGGCTAAAATCGACCcgcggacccccgc                                                                                                                                         >  2:333668/1‑229 (MQ=255)

                                                                                                     cGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCATTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTT‑TCGCCGGAGAAA‑CTCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCCGCAAACCGTCCTGGCTAAGGTCGACCACCGAGCCAccac                                                                                                                                         >  2:992193/1‑235 (MQ=255)

                                                                                                        aaCGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGAACATCTCTTTTTTACCAAC‑AATAATTCCTGCCCGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑ATCACCCGACTGACGACCGCCGCCATCAACTCCCTCGGCATTGGCTCCTTCGGACAAACGGACTTGCCAAGATCAACCAGCGGACCACTGCCTaa                                                                                                                                     >  2:1052962/1‑239 (MQ=255)

                                                                                                          cGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTTGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTaa‑a                                                                                                                                   >  1:1195067/1‑238 (MQ=255)

                                                                                                               gCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCCCCGGAGAAA‑ATCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCTGCAAACCGTACTGGCTAAGATCGACCAGCCAGCCACTGCCTAA‑ATCAg                                                                                                                               >  1:1129987/1‑238 (MQ=255)

                                                                                                                catgtgtgtataagagacagc‑ATTTTATCCCGGGGTAATGCCGGCTCAGGCGGGTGGTTTGGCGGGGGGCCGACCATCTTTTTTTTCCCAAA‑AAAAATCCCTCCCTGCGG‑CCCGCCAAAAA‑ACTTG‑TCGCCGGAAAAC‑CCCACCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGt                                                                                                                              <  2:566524/219‑1 (MQ=255)

                                                                                                                cTTCC‑AGGGCGGTGGGTGTC‑TTTTTCGCCGCGATTAATGCACGCGTAGGCGGGGGGCTTGCCAGGCGGGCGATCAGCTCTTTTTTACAAAA‑AAGTATCCCGGCCTGCGG‑CCCTCCTAAAA‑TCTTT‑CCGCCGGAAAAA‑CTCCCCAGACTGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGt                                                                                                                              <  2:199879/238‑1 (MQ=255)

                                                                                                                cTTCC‑AGCGCCGCGAGGGTC‑ATTTTTTCCGCCCTTAATCCCCGTTTCAGCGGGTGGTTTTGCGGGCGGGCGATCATCTCTTTTTTCCCAAC‑AATAATCCCTGCCTGCGC‑CCCGCCTAACA‑CCTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGt                                                                                                                              <  1:1443567/238‑1 (MQ=255)

                                                                                                                   cc‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGAAGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCCAAGATCGACCAGCGAGCCACTGCCTAA‑ATCCGTCACt                                                                                                                          >  1:764939/1‑239 (MQ=255)

                                                                                                                   cc‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAAAA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGa                                                                                                                                                            >  1:1324371/1‑206 (MQ=255)

                                                                                                                       gcgcCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATAGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACg                                                                                                                       >  1:1119825/1‑239 (MQ=255)

                                                                                                                          ccgcGAGGTTT‑ATTTTCTCCGCCTTTAATGCCCCCTCCGGCGGTGGTGTTGGCAGGCGCGCGTTCCTCTTTTTTTTACCAAC‑AAAATTCCCCGCCGGCGC‑CCCCCCAAACA‑ACTTG‑CCCCGGGAGAAC‑CTCCCCAGACGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGa                                                                                                                    <  2:764939/239‑1 (MQ=255)

                                                                                                                           cgggggggTC‑TTTTTACCCGCGCGTAAGCCCCGCTTCAGGGGGGGGTTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAA‑AATAATCCCTGCCTGCGC‑CCCCCCTAACA‑ACTTG‑TCGCCGGAAAAA‑CCCACCAGACGGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGa                                                                                                                    <  2:228301/233‑1 (MQ=255)

                                                                                                                                gtgTC‑ATTTTATCCGCGCGTAATGCACGCTTAGGCGGGTGGCTTTGAAGGCGGGCGTTCATCTTTTTTTTACCAAC‑AATAATCCCCCCCTGCGC‑CCCGCCTAACA‑ACTTG‑CCGCGGGAGAAA‑CTCCCCAGCCTACCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATc                                                                                                               <  2:1212827/236‑1 (MQ=255)

                                                                                                                                 gggattttttACCCGCGCTAAATGCCCGCTAGGGCGGGGGGTTTGGCGGGCGGGCGTCCATCTCTTTTTCCCCAAC‑AAAACTCCCTGCCGCCGC‑CCCGCCAAACA‑CTTTT‑CCGCCGGAAAAC‑CTCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATc                                                                                                               <  2:1081923/232‑1 (MQ=255)

                                                                                                                                            tCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACCAAC‑CATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑ATCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCCTTCGGCAAACCGTACTGGCCAAGATCCACCACCGAGCCACTGCCTCA‑ATCAGTCACTACGGGAACATCCCGCTCATTGc                                                                                                    >  2:721638/1‑238 (MQ=255)

                                                                                                                                               gcgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCATTCAACTCCTGCGGCATTGGCTCTTTCCGCAAACCGTACTGGCTAAAATCGACCCGCGCGCCACTGCCTAA‑ATCAGTCCCTACGGGAAACTCCAGCTCTTTGCCGa                                                                                                 >  2:354434/1‑238 (MQ=255)

                                                                                                                                                cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACCCAC‑AATAATTCCTGCCTGCGG‑CCCCCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCCGACTGACGCCCGCCGCAATCAACTCCCGCGGCATTGGCTCTTTCGGCAAACCCCACTGGCTAAGATCGACCAGCGAGCCACTCCCTAA‑ATCAGTACCTACGGGAACATCCACCTCTTTGCCgcc                                                                                                >  2:120531/1‑236 (MQ=255)

                                                                                                                                                cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGGGCCACTGCCTAA‑ATCAGGCCCTACGGGAACATCCAACTCTTTGCCCAgc                                                                                               >  1:1424343/1‑239 (MQ=255)

                                                                                                                                                cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGa                                                                                                                    >  1:566524/1‑218 (MQ=255)

                                                                                                                                                cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAgc                                                                                               >  1:1305327/1‑239 (MQ=255)

                                                                                                                                                cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCCGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAg                                                                                                >  1:153672/1‑238 (MQ=255)

                                                                                                                                                  cgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑GCCGCCTAACA‑ACCTG‑TCGCCGGAGAAA‑CTCACCAGACTTCCCCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTTGGCAAACCGTACCGGCTAAGATCCACAACCCAGCCACTGCCCAACATCA‑TCACTACGGGAACATACCGCTCTTCGACCAgcg                                                                                              >  2:1243927/1‑238 (MQ=255)

                                                                                                                                                    tAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCCGCTCTTTGCCGAGCGcc                                                                                            >  1:1337349/1‑238 (MQ=255)

                                                                                                                                                          aCGCTTCAGCGGGGGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGC‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGtt                                                                                     <  1:1337184/239‑1 (MQ=255)

                                                                                                                                                             cTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGAACGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTcc                                                                                   >  1:44235/1‑238 (MQ=255)

                                                                                                                                                             cTTCAGCGGGTGGCTTTGCAGGCGGGAGATCATCCCTTTTTTACCCAC‑AATAATTCCTGCCCGCCG‑CCCGCCTAACA‑ACTTG‑TTGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCCCCATCCACCCCTGCGGCATTGGCCCTTTCCGCAAACCGGACTGGCTAAGATCCACCAGCGGGCCACTGCCTAA‑ATCCCTCACCACGGGAACACCCAACTCTTTGCCGACAGCCCCCAGTTcc                                                                                   >  2:682729/1‑238 (MQ=255)

                                                                                                                                                               tCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCt                                                                                >  1:714215/1‑239 (MQ=255)

                                                                                                                                                                 aGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACCACGGGAACATCCAGCTCTTTTCCGAGCGCCCCCCGCTCCCCtt                                                                               >  2:1099981/1‑238 (MQ=255)

                                                                                                                                                                   cGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACCTGCTAAGATCGACCCGCGCGCCACTGCCCAA‑ATCAGTCACTACGGGAACATACAGCTCTTTGCCGAGCGCCACCCGTTTCCCTTCa                                                                             >  2:1337184/1‑238 (MQ=255)

                                                                                                                                                                        ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCCAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTTGCTCTTTCGGCACACCGTACTGGCTAAGATCGACCAGCGAGCCCCCGCCTCA‑ATAACTCACTACGGGAACATACAGCTCTTTTACGCGCGCCACCAGTTCCCCTTCATCTaa                                                                        >  2:918063/1‑237 (MQ=255)

                                                                                                                                                                        ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTTGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCCCCCGTTCCGCt                                                                                >  1:1103567/1‑230 (MQ=255)

                                                                                                                                                                        ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGc                                                                                                                                                                 >  2:1258393/1‑150 (MQ=255)

                                                                                                                                                                        ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATCCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGc                                                                                                                                                                 <  1:1258393/150‑1 (MQ=255)

                                                                                                                                                                                  gcgggcaATCACCTCTTTTTTACCAAC‑AAAAATTCCTGCCTGCGG‑CCCGCCAAACA‑ACTGG‑TCGCCGGGGAAC‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg                                                              <  1:333668/238‑1 (MQ=255)

                                                                                                                                                                                        gtacattctttttttCCCAAA‑AATATTCCCGGCCCGCGC‑CCCGCTAAACA‑CCTTG‑TCCCCGGAGAAC‑CCCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                       <  2:659711/230‑1 (MQ=255)

                                                                                                                                                                                        gATCATCTTTTTTTTCCCAAA‑AATATTTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                       <  1:1070077/239‑1 (MQ=255)

                                                                                                                                                                                        gATCATCTCTTTTTTACCAAC‑AAAAATCCCTGCCGCCGC‑CCCGCCAAACA‑ACTTG‑CCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                       <  1:501263/239‑1 (MQ=255)

                                                                                                                                                                                        gATCATCTCTTTTTCCCAAAC‑AATAATCCCTGCCTGCGC‑CCCGCCAAACA‑CCTGG‑GCGCCGGAGACC‑CCCACAAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                       <  1:1161642/239‑1 (MQ=255)

                                                                                                                                                                                         acactctcTTTCTTCCCACC‑AATATTTCCCGCCGGCGG‑CCCCGCGATCC‑TCTTG‑T‑GTCGGAAAAAGCCCCCCAGGGGGCCCCCCGCGGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACGGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                       <  2:505471/234‑1 (MQ=255)

                                                                                                                                                                                               ttttttttCCCAAC‑AATAATTCCGGCCGGCGG‑CCCGCCTAACA‑CCTGG‑TCCCCGGAGAAC‑CCCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt                                                <  1:1028165/237‑1 (MQ=255)

                                                                                                                                                                                               ttttttttACCAAA‑AATAATCCCGGCCGGCGG‑CCCGCCAAAAA‑ACTGG‑TCGCCGGAGAAC‑CTCACCAGACGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt                                                <  1:992851/237‑1 (MQ=255)

                                                                                                                                                                                               tctTTTTAACAAAC‑AATACTCCCGGCCGGCGC‑CCCCACTAACA‑CTTTG‑CCCCCGGAAAAC‑CCCCCCAGCCGCCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt                                                <  2:1150432/239‑1 (MQ=255)

                                                                                                                                                                                                ctTTTTTTCCCAC‑AAATACTTCTGGCTTCGGCCCCGCCAAACA‑ACTAT‑TCGCGGGAAAAA‑CCCCCCAGCCGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt                                                <  2:812378/239‑1 (MQ=255)

                                                                                                                                                                                                ctTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCTGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAAt                                                   >  1:1217508/1‑235 (MQ=255)

                                                                                                                                                                                                 ttttttCCCAAA‑AATAATTCCGGCCGGCGG‑CGCGCGTAACA‑ACTTG‑CCGCCGGAGAAC‑CCCCCCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGt                                              <  1:121383/239‑1 (MQ=255)

                                                                                                                                                                                                   ttttACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAg                                            >  1:307231/1‑239 (MQ=255)

                                                                                                                                                                                                    tttACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGcca                                                                                           >  1:625617/1‑191 (MQ=255)

                                                                                                                                                                                                    tttACCAAC‑AATAATTCCTCCCTGCGG‑CCGCCATACCA‑ACTTG‑TCGCCGGAGAAC‑CTCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGt                                           <  1:621237/239‑1 (MQ=255)

                                                                                                                                                                                                           ac‑aaTAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt                                    >  2:1382038/1‑239 (MQ=255)

                                                                                                                                                                                                                 cttccctcccggCGG‑CCCCCCAAACA‑CTGTG‑TCGCGGGAAAAC‑CCCCCCGGACTCACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                               <  2:1305327/235‑1 (MQ=255)

                                                                                                                                                                                                                 actccctgccggGCG‑CCCGCCTAA‑A‑ACGTGTTCGCCGAAAAAC‑CCCACCAGCCAGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                               <  2:714215/235‑1 (MQ=255)

                                                                                                                                                                                                                 actccccccctgcGC‑CCCGCCAAACC‑ATGTG‑CCGCGGGAAAAA‑CCCCCCAGCCTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                               <  2:114565/231‑1 (MQ=255)

                                                                                                                                                                                                                 aatCCCTGCCTGCGG‑CCCGCCTACCA‑CCTTC‑CCCCCGGAGAAC‑CCCACCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                               <  2:655659/239‑1 (MQ=255)

                                                                                                                                                                                                                 aatCCCTGCCTGCGG‑CCCACCTAACA‑TGTTG‑TCCCCGGAGAAC‑CTCCCCGGACTCACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                               <  1:1098974/239‑1 (MQ=255)

                                                                                                                                                                                                                  aactccggccgcggaccccccTAACA‑ACTTG‑ACGCCGGACAAA‑CCCCCCGGACGGACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                               <  2:73351/224‑1 (MQ=255)

                                                                                                                                                                                                                   tccctgcctgCGT‑CCCGCCAAACA‑CCTGG‑GCGCGGGAGAAA‑CCCACCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACt                             <  2:1387665/237‑1 (MQ=255)

                                                                                                                                                                                                                        gcctgcGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                         >  1:998697/1‑238 (MQ=255)

                                                                                                                                                                                                                        gcctgcGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAAACCCTGAATGCTGTAGTTACTGGTATGTAATTtct                         >  2:389834/1‑237 (MQ=255)

                                                                                                                                                                                                                             cGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTAc                              >  1:1035725/1‑228 (MQ=255)

                                                                                                                                                                                                                              gg‑CCCACCTAACA‑ACTTG‑CCGCCGGAAAAA‑CTCACCAGCCTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                   <  1:1369943/238‑1 (MQ=255)

                                                                                                                                                                                                                                                 g‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTCGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc  >  2:1353282/1‑238 (MQ=255)

                                                                                                                                                                                                                                                 g‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGCCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc  >  2:973765/1‑238 (MQ=255)

CTGTAAACGTTGTGCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGT‑AAATAAAGACGCAACGTG‑GCTTCC‑AGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAC‑AATAATTCCTGCCTGCGG‑CCCGCCTAACA‑ACTTG‑TCGCCGGAGAAA‑CTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAA‑ATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTC  >  NC_000913/3760133‑3760604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`