Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,862,669	T→A	17.9%	pseudogene (934/1617 nt)	glvC ←	pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,862,669	0	T	A	17.9%	83.6 / 6.0	68	pseudogene (934/1617 nt)	glvC	pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base T (27/22);  new base A (6/4);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

ATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAA  >  NC_000913/3862434‑3862903                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GGATT‑AAGGT‑TG‑‑GGGAATCac                                                                                                                                                                                                                                                      >  2:248536/1‑238 (MQ=255) aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TGC‑GGGGATCa                                                                                                                                                                                                                                                       >  1:185378/1‑238 (MQ=255)         cAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGG‑TGCGGGGAATCacaaaccc                                                                                                                                                                                                                                                >  1:502906/1‑231 (MQ=255)                atatCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAGGT‑TGC‑GGGAATCA‑A‑C‑AAGcccc‑ccccctt                                                                                                                                                                                                                                   >  2:1081795/1‑234 (MQ=255)                       cgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAAGG‑TGC‑GGGGATCA‑G‑C‑AAACCCC‑CCA‑C‑TTTTT‑CCCgg                                                                                                                                                                                                                         >  1:1097435/1‑238 (MQ=255)                           gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGTGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑TAGGT‑TGC‑GGGAATCA‑G‑C‑AAACCCG‑CCA‑C‑tttttacccttttt                                                                                                                                                                                                                      >  1:109894/1‑229 (MQ=255)                           gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑TAAGT‑GGC‑GGGGATCA‑G‑C‑AACACCC‑CCC‑C‑TTTTT‑CCCCAttt                                                                                                                                                                                                                      >  1:773953/1‑238 (MQ=255)                           gTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCACATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAACAACGGAGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GA‑AAACT‑TGC‑AGGGACAA‑A‑C‑AAGCTCG‑CCC‑C‑ATTTA‑ACCCAtttt                                                                                                                                                                                                                     >  2:1040278/1‑239 (MQ=255)                                 gaccttgggaacccAGTTGGGCGGAAACACGTAGTAAACCAGCCTCCCGCCCATGTGGCCCACCACCCAAAAGAGAAAAATCACGGTCGAAAGTGAGGCCACGAGCCGGGCGTGTCCCCCAAACAGCAACGTGAAAAAGAAGAGGAAGGAAAATCCCGGCGGTTGGGTAATCCCCCCCAGCAGGGC‑GG‑TT‑AAGGT‑GGG‑GGAAATAA‑G‑G‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTg                                                                                                                                                                                                               <  2:366749/227‑1 (MQ=255)                                  aaCGTCGGGATCCGTTTTTGCAGAAAACCCTAGCCAATCAGCCCCCCCCCCATGTCCCCCACCACACCAAAGAAATACATGCCGGTCGACATGGAGGCCGCCAGCACGGGGTGTACCGCAAACAGCAACGGGGAAATGAACAGGAAGTTAAATCCCGGCGGTTGGGTAATCCCCCCAAGCTGGGG‑GG‑TT‑AAGGT‑TGG‑GGAAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTg                                                                                                                                                                                                               <  2:835676/238‑1 (MQ=255)                                   cgggcGGGATCCAGTTTCGCGAAAAACCCTACCAAACCAGCCCCCCCCCCATTGTCCCCCCCACACAAAAGAGATACATTACGGTCGACATGGAGGCCGCCGGCACGGCGTGACCCAAAAACAGCAACGGTGAAATGAACGGGAAGGAAAACCCCGGCGGTTCGGTAATCCCCACCAGAAGGGG‑GG‑TT‑AGGGT‑GGG‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTg                                                                                                                                                                                                               <  2:639637/233‑1 (MQ=255)                                         ggATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑TA‑AAGGT‑GGG‑GGGAATAACG‑A‑AAGCGC‑‑CCC‑C‑TTTTA‑‑CCAATTTTCTGGTgggggcgag                                                                                                                                                                                                       >  2:1249681/1‑234 (MQ=255)                                              cattttgGCGGAAAAACATCGTAAACCACACCCCCCCCAATGTCCCCCACCACACAAAAGAAATACATCGGGGTCGACATTGAGCCCGCCAGCAGGGCGTGTCCCGCAAACAGCAGCGGTAAAAGGAAAGGGAAGGTAAATCCCAGCGGTTCGGTAACCCCCCCCAGCAGGGC‑GG‑TT‑AAGGT‑TGC‑GGAACTAA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTa                                                                                                                                                                                                 <  2:207405/236‑1 (MQ=255)                                              cagtttggtgaaaaaaCCTAACCAACCAGCCCCCCGCCCATGTCCCCCCCCACCCAAAATAGAAACATTCGGGTGTAAGTGGCGGCCACCAGCAGGGCGTGTGCCGAAAACAGAAAGGGTAAAAGGAACGGGATGATAAGTCCCAGCGGTGGGGAAATCCCCCCCAGTTTGCG‑GT‑TT‑AAGTT‑TGC‑GGAAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTa                                                                                                                                                                                                 <  2:524086/227‑1 (MQ=255)                                               aGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGG‑TTC‑GGGGATCA‑G‑C‑AAGCCCC‑CCA‑C‑TTTTA‑CCCCATTTTTCTGTGCGGC‑AGTGAAGTAc                                                                                                                                                                                                >  1:762962/1‑239 (MQ=255)                                                 ttttGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGGT‑GGG‑GGGAAT‑A‑G‑C‑AAACCCG‑CCC‑C‑CTTTT‑CCCGATTTTCCGGTgggggcggggaggcg                                                                                                                                                                                                 >  1:639637/1‑226 (MQ=255)                                                 tttgggcaaaaaaCCTATACACTCAGCCCCCCGCCCAGGTCCCCCCCCACCCAAAAGAGAAACATTACGCGCGAAATGGAGGCCGCCAGCAGGGGGTGTACCGAAAACAGAAACGGGAAAAGGAACAGGAGGTTAAATCCCGGCGGTGCGGTAATCCCCACCAGCATGGG‑GG‑TT‑AGGGT‑GGG‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACAt                                                                                                                                                                                              <  2:1041887/230‑1 (MQ=255)                                                  tttGGGATAAACCCGGGTCAACCCGACCCCCCCCCTTTTGCCCCCCCCCACAAAGGACATACTTTAGGGTGGACATGGAGGCCGCCAGCCCGGCGGGACCCCAAAACAGACACGGAGAAATGATAAGGAGGGAAAGTCCAAGCGGAGCGTTACTTCCCCCCGGCATGGG‑GT‑TT‑AGGGT‑GGG‑GGGAAGAA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTAc                                                                                                                                                                                                <  2:874743/236‑1 (MQ=255)                                                   tgcgggaaaaaCCTAGTAAACCAGCCCTCCGCCCATGTCGCCCCCCACACAAAAAAGATACATTACGGTGGAAATTGAGGCCGCCGGCCCGGCGTGAACCGAAACCACAAAGGGAGAAAGAAACAGGAAGTAAAATCCCAGCGGTTCGGTAATCCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TGG‑GGAAAACA‑G‑A‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCg                                                                                                                                                                                            <  2:1097435/232‑1 (MQ=255)                                                           aaaCCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAAGG‑TGT‑GGGGATCA‑G‑C‑AAAACCG‑CCC‑C‑TTTTT‑TCCGATTTTTCCGTGCGGC‑GGTGGAGGACATCGCTaaaaaa                                                                                                                                                                                    >  1:231935/1‑239 (MQ=255)                                                                   tCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑TT‑AAGGT‑TGG‑GGGAA‑CA‑A‑C‑AAAGCCC‑CCC‑C‑TTTTATCCCGTTTTTCCGGTGGGGC‑GGGAAAGAAC‑TCGCCAAAGAAAAGcccc                                                                                                                                                                             >  2:316051/1‑237 (MQ=255)                                                                       tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAAGG‑TGG‑GGGGATCA‑G‑C‑AAGCCCC‑CCC‑C‑CTTTT‑CCCCATTTTCCGGGGCGGG‑AGGGGAGTACAACCCCTAAAAAAAGCCCCCGGc                                                                                                                                                                         >  1:136869/1‑238 (MQ=255)                                                                       tCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑CT‑AAGGA‑TTC‑GGGGATCC‑G‑C‑ACACCCC‑CCC‑C‑CTTTA‑CCCCAATTTTCCGTGCGGC‑GGTGAATGACATCGCCAACAAAATGctcccgac                                                                                                                                                                         >  2:517063/1‑231 (MQ=255)                                                                        cAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGG‑TGC‑GGGGATCA‑G‑C‑AAGCCCC‑CCC‑C‑TTTTA‑ACCGCTTTTCCGGGGGGGG‑AGTGAAGGACATCGCTAAAAAAATGCCCACGGCa                                                                                                                                                                        >  1:968735/1‑238 (MQ=255)                                                                           aCCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TTC‑GGGGAACA‑G‑CAAACCCCC‑CCC‑C‑TTTTA‑CCC‑ATTTTTCGGTGCGGC‑AGTGGAGTACATCGcctgtctcttttacccatcctcgg                                                                                                                                                                    >  1:366214/1‑216 (MQ=255)                                                                                cGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAGGG‑TGC‑GGGGAACA‑G‑C‑AAACCCG‑CCC‑C‑TTTTT‑CCCCGTTTTTCGGTGCGGG‑CGGGGAGTACACCGCCAAAAAAAAGCCCCCCGGCCCCAAGAtt                                                                                                                                                               >  1:535343/1‑239 (MQ=255)                                                                                     gaaaaagaacaagccaaaaaGAGAAAAATGACGGTCGAAATGGAGGCCGCCAGCAGGCGGTGCCCCAAAAACAGAAACGGTAAAAAAAACAGAAGGAAAAATCCCGGCGGTTGGGTAATTCCCACCAGCATGGC‑GT‑TT‑AAGGT‑TGG‑GGAAATCA‑G‑C‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTg                                                                                                                                                           <  2:890572/221‑1 (MQ=255)                                                                                      tGTTGCCCACCACACCAAAGAAATACATTACGGACGACATTGAGGCCGCCAGCACGGCGTGTCCCGCAAACAGCAACGGTGAAATAAACAGGAAGGAAAATTCCAGCGGTTCGGTAATCCCCACCAGCATGGC‑GT‑TT‑AAGGT‑TGC‑GGGAATCA‑G‑A‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑g                                                                                                                                                        <  1:1037355/239‑1 (MQ=255)                                                                                      ggtcccccACCACCCAAAAGAGATACATTACGGTCGACATGGAGGCCGCCAGCACGGGGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGATAAATTCCAGCGGTTCGGTAACTCCCACCAGCAGGGC‑GG‑TA‑AGGGT‑TGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTg                                                                                                                                                           <  1:1427858/232‑1 (MQ=255)                                                                                              accacaccaAGGAGCAACATTGCGGTAGGAATGGGGGCCGCCGGCACGGCGGGTCCCGCAAACAGCAACGGTAAAATGAAAAGGAAGGTAAATCCAAGGGGTTCGGTAATCCCCACCAGCAGGGC‑GT‑TT‑AGGGT‑GGG‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑g                                                                                                                                               <  2:1098257/239‑1 (MQ=255)                                                                                                cacaccaAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAAGT‑TGC‑GGGGAACA‑G‑C‑AAGCCCC‑CCA‑C‑TTTTA‑CCCGATTTTTCGGGGGGGC‑GGGGAAGGAAAACGCTAAAAAAATGCCCACCGCACCACAGAATTTTGAGGTTACCGT‑ga                                                                                                                                              >  1:1151775/1‑238 (MQ=255)                                                                                                   accaAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCGCGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATCCCCCCCAGCAGGGC‑GG‑TT‑AAGGT‑GGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCAc                                                                                                                                                                            <  1:44161/207‑1 (MQ=255)                                                                                                     caAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAAGG‑TGC‑GGGGATCC‑G‑C‑AAACCCG‑CCC‑C‑TTTTT‑CCCGCTTTTTCCGGGCGGC‑AGGGAGATACATCGCCAAAGAAACGGCCCCCGCACCCAAACTATTTTA‑GGTACCCT‑GCA‑gggga                                                                                                                                       >  2:1030318/1‑237 (MQ=255)                                                                                                            aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGGAGTACATCGCTAAAGAAATGCCCACGGCACCCAAGATTTTTGA‑GTTACCGT‑GCA‑CGG‑GA‑AAACCg                                                                                                                               >  2:770245/1‑238 (MQ=255)                                                                                                             tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGG‑TGC‑GGGGATCA‑G‑C‑AAACCCG‑CCC‑CTTTTTC‑CCCGTTTTTCCGG‑GCGGG‑AGGGGAGGAAACCGCCCAAAAAAAGCCCCCCGGCCCCAAGATTTTTTT‑GTTTCCCTGGCA‑GGG‑CA‑AAAccc                                                                                                                               >  1:696034/1‑237 (MQ=255)                                                                                                             tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAAGG‑TGC‑GGGGATCA‑A‑C‑AAACCCC‑CCACC‑TTTTC‑CCCCTTTTTCCGG‑GGGGC‑AGTGGAGAACAACGCTAAAGAAAAACCCACCACACCAAAAAATTTTTG‑GTTAACGG‑Gcccgtttcttttaca                                                                                                                                >  1:890572/1‑225 (MQ=255)                                                                                                               cATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGGGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAAGG‑TGC‑GGGGAACA‑G‑C‑AAACCCC‑CCC‑C‑TTTTA‑CCCCATTTTTCGGGGCGGG‑AGTGAAAGACAACGCCAAAAAAATGACCACGGCACCAAAAATTTTTTA‑GTTACCCT‑GCACGGG‑CA‑AAAcccct                                                                                                                             >  2:537825/1‑235 (MQ=255)                                                                                                                    cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑TA‑AAGGT‑GCC‑GGGAACAA‑C‑C‑AAGCCC‑‑CCC‑C‑CTTTA‑TCCCATTTTTCGGGGCGGCGCGGAAAGAACAACCTCAAAAAAATCCCACCGGCCCCAAAAATTTTTTA‑GTTTCCGG‑GAG‑GGG‑AC‑AAACCCCCTTcccg                                                                                                                       >  2:491274/1‑237 (MQ=255)                                                                                                                    cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GGATG‑AAGGT‑TG‑‑GGGAATTA‑G‑G‑AAACCCC‑CCA‑C‑CTTTT‑CCCGATTTTTCCGGGGGGG‑AGTGGAGTAAAACGCTAAAAAAAAACCCACAGCACCAAAGAATTTTGA‑GATAACGT‑GGC‑AGG‑CA‑AAAACGCCTTCCggg                                                                                                                      >  2:1382028/1‑239 (MQ=255)                                                                                                                      gTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGG‑TGG‑GGGGATCA‑G‑CAAAGCCCC‑CCC‑T‑TTTTA‑CCC‑ATTTTTCCGTGCGGC‑CGTGGAGGAAAACGCCAAAAAAAAGCCCCCCGCCCCCAAGAATTTTTA‑GGTACCGG‑GCA‑GGGCCA‑AAACCCCCTCCCGGGa                                                                                                                     >  1:1048976/1‑239 (MQ=255)                                                                                                                         gACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑TAGGG‑TGC‑GGGAATCA‑A‑C‑AAAACCGCCCC‑C‑TTTTC‑CCCCATTTT‑CGGTGCGGG‑CGTGAAGGACAACCCTAAAAAAAAACCCACCGCACCCCAAATTTTTTA‑AGTAACGT‑TGA‑GGG‑CA‑AAACCCCCTCCCGGGaaaca                                                                                                                 >  2:1424731/1‑236 (MQ=255)                                                                                                                         gACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GT‑AAGGT‑TGC‑GGGGAACA‑G‑C‑AAGACCC‑CCA‑C‑CTTTT‑CCCGATTTTTCGGTGCGGC‑AGGGAAGTAACACGCTAAAGAAAAGCCCCCCGCACCAAAGATTTTTTA‑GGTAACGG‑GCC‑AGG‑GA‑AAACCCCCCTCCCGGaacaa                                                                                                                 >  2:234796/1‑239 (MQ=255)                                                                                                                                     gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGG‑TTC‑GGGGAACA‑G‑C‑AAGCCCC‑CCA‑C‑ATTTA‑ACCGATTTTTCGGTGGGGC‑AGGGAAAGAAAATGCCAAAAAAAAGCCCCAGGCCCCAAAAATTTTTTG‑GTTCCCGG‑CCG‑GGG‑CA‑AAACCCCCTTCCGGGAAAAACAAttctaccg                                                                                                      >  2:1072488/1‑232 (MQ=255)                                                                                                                                     gccAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAAGG‑TGT‑GGGAATCA‑GAA‑AAGCCCC‑CCC‑C‑TTTAC‑CCCCATTTTCCGG‑GCGGG‑GGGGAAGGACAACGCCAAAAAAAAGCCCCCCGCACCCAAAATTTTTTG‑GTTATCCT‑GTA‑TGG‑CACAAAACCCCTCCCGGGagaaaaatttataccg                                                                                                      >  2:402350/1‑224 (MQ=255)                                                                                                                                                       caaaaCAGCAAGGGTGAAATGAACAGGAAGGAAAATCCCGGCGGTTCGGAAATTCCCACCAGCATGGC‑GG‑TT‑AGGGT‑GGG‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGaa                                                                                   <  1:994202/237‑1 (MQ=255)                                                                                                                                                       aaaaaCAGCAGCGGTGAAATGAACAGGAAGGTAAATCCCAGCGGTTCGGTAATCCCCACCAGCATGGG‑GG‑TT‑AAGGT‑TGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGaa                                                                                   <  1:1438721/237‑1 (MQ=255)                                                                                                                                                        cAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAAGT‑TTC‑GGGGATTA‑A‑C‑AAACCCC‑CCC‑C‑CTTTT‑CCCGGTTTTTCGGTGCGGC‑AGGGAAGTAAATCGCCACAAAAAAACCCCCAGCACCAAACACTTTTTA‑GTTACCCC‑TCA‑GAG‑CA‑AAACCGCCTGCCCGGGACAACCAATTTAGCGGAGCGGCGCtacacactca                                                                                   >  2:1386523/1‑229 (MQ=255)                                                                                                                                                           aaaGCAACGGTAAAAGGAACATGAAGGTAATTCCCAGCGGTCCGGAATCCCCCCCCAGCGTGGG‑GG‑TT‑AAGGT‑GGG‑GGAAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAActct                                                                               <  2:136869/237‑1 (MQ=255)                                                                                                                                                                aaCGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TTC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCCCCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCCCCAACTGAACTCTTGccg                                                                          >  1:309975/1‑237 (MQ=255)                                                                                                                                                                  cGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TGC‑GGGAATCA‑G‑C‑AAGACCC‑CCC‑C‑CTTTA‑CCCGATTTTCCGGTGCGGC‑CGGGAAGTACATCGCTCAAGAAATTCCCACGGCACCCAAGATTTTTTA‑GGTAACCC‑GCA‑GGG‑GA‑AAAACGCCTTCCGGGAAAAACGATTTCCACGGCCCGGAGCCCAAACAGaaacacttcagat                                                                        >  2:446498/1‑228 (MQ=255)                                                                                                                                                                      gaaaagacagggagtgaaatctCCAGCGGTAGGGTAA‑TCCCCCCCGCCGGGG‑GG‑TT‑AAGGTGTGC‑GGAAACAA‑A‑A‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGAtgct                                                                     <  2:968735/217‑1 (MQ=255)                                                                                                                                                                            aaaagaaaGGAAAATCCCGGCGGTCGGGAAATTCCCCCCAGCATGGC‑GT‑TA‑AGGGG‑TGC‑GGGAACCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                <  1:530942/231‑1 (MQ=255)                                                                                                                                                                            aaaagaaaGGAAAATACCAGCGTTTGGTAAATCCCCACCAGCAGGGG‑GG‑TT‑AGGGT‑GGG‑GGAAAACA‑G‑A‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                <  2:1419186/231‑1 (MQ=255)                                                                                                                                                                            aaaaGGAAGGTAAATTCCAGCGGTTCGGTAATCCCCACCAGCATGGC‑GA‑TT‑AGGGT‑GGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                <  1:770245/234‑1 (MQ=255)                                                                                                                                                                             agaggatgtaaaaTCCCAGCGGTACGGTACTCCCCACCGGCATGGC‑GG‑TT‑AAGGT‑TGC‑GGAAAGCA‑G‑C‑AGGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAg                                                             <  1:780352/229‑1 (MQ=255)                                                                                                                                                                             aCAGGAAGTTAAAGTCCAGCGGTTCGTTAATCCCCCCCAGCATGGC‑GG‑TT‑AAGGG‑TGC‑GGGAATAA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAg                                                             <  1:650117/239‑1 (MQ=255)                                                                                                                                                                                 gAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑TAAGT‑TGC‑GGGAATCA‑G‑C‑AAACCCC‑CCC‑C‑TTTTT‑CCCCATTTTCCGGTGCGGC‑CGTGAAGTACATCGCTAAAAAAATTCCCACGGCACCAAAAATTTTTTA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACCACGATTTCAGCGGCTCCGCGCTCCAACTTAAATCCTGCAGATGCTCTGccccctac                                                          >  2:530942/1‑233 (MQ=255)                                                                                                                                                                                                ggtttggttAATCCCCCCCAGCAGGGC‑GG‑TT‑AGGGT‑TGC‑GGAAAACA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCt                                           <  1:316051/230‑1 (MQ=255)                                                                                                                                                                                                          actccaccaagaggggC‑GG‑TA‑AGGGT‑GGC‑GGAAACAA‑G‑C‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGc                                <  2:696034/225‑1 (MQ=255)                                                                                                                                                                                                               caccaGAATGGC‑GG‑TA‑AAGGT‑GGG‑GGGAATCA‑G‑A‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGAc                           <  1:9660/239‑1 (MQ=255)                                                                                                                                                                                                               cacaaGGATGGG‑GG‑TT‑ACGCG‑TGT‑GGGAATGC‑G‑A‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTGCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATTCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACATCTGCCGGAc                           <  2:107189/239‑1 (MQ=255)                                                                                                                                                                                                                           ggg‑TT‑AGGGT‑TGG‑GAGAAGCA‑G‑C‑AGGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                 <  1:84386/237‑1 (MQ=255)                                                                                                                                                                                                                               gtgttaatggtgggcaaaTCA‑G‑C‑AACCCCG‑CCA‑C‑TTCTA‑CCCGATTTTCCTGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACTATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATaaa                 <  2:1066027/222‑1 (MQ=255)                                                                                                                                                                                                                                       gggC‑GGGAAACA‑G‑C‑AAGGCCG‑CCA‑C‑TTTTA‑CCCGGTTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATGTTTGA‑GTTACCGT‑GCA‑GTG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGt         <  2:1369119/235‑1 (MQ=255)                                                                                                                                                                                                                                          c‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATg    >  2:1442183/1‑239 (MQ=255)                                                                                                                                                                                                                                             ggAATCACG‑C‑AGGCCCG‑CCA‑C‑TTGTT‑CCCGATTTTCCGGGGCGGC‑GGTGAAGTACATCGCTGAACAAATGCCCACGGCACCAAAGAGGTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCTGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCTCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATg    <  2:1285724/238‑1 (MQ=255)                                                                                                                                                                                                                                              gAATCA‑G‑C‑AACCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa  <  1:472038/238‑1 (MQ=255)                                                                                                                                                                                                                                              aaaTAA‑G‑C‑ACCCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGaa  <  1:234796/237‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                        ATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TT‑AAGGT‑TGC‑GGGAATCA‑G‑C‑AAGCCCG‑CCA‑C‑TTTTA‑CCCGATTTTCCGGTGCGGC‑AGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGA‑GTTACCGT‑GCA‑GGG‑CA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTACATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAA  >  NC_000913/3862434‑3862903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑