BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,027,131	A→G	14.2%	G202G (GGA→GGG)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,027,131	0	A	G	14.2%	295.1 / 6.2	117	G202G (GGA→GGG)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand): ref base A (47/46); new base G (15/5); total (63/54)
Fisher's exact test for biased strand distribution p-value = 5.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TGGCGCGTAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGA  >  NC_000913/4026902‑4027367

tGGCGCGTAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑gag                                                                                                                                                                                                                                          <  2:505487/239‑1 (MQ=255)

 ggCGCGTAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑gag                                                                                                                                                                                                                                          <  2:389682/238‑1 (MQ=255)

       tAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGGTCAC‑GGTACGC‑CGGGAGGGCATGGTATTTATA‑TTTTCGG‑GCGGTTT‑TAGG‑Tggggg                                                                                                                                                                                                                                  >  1:615595/1‑236 (MQ=255)

           ccAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAAt                                                                                                                                                                                                                              <  1:1382302/239‑1 (MQ=255)

               aCCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTGCAGGATCAC‑GGTACGC‑TCGCAGAGCATGGTATCCATA‑TTGCCGG‑GCGTTTT‑GCGA‑AGGGGGAAATTgt                                                                                                                                                                                                                           >  1:683621/1‑237 (MQ=255)

                    gATATCACCATTTATTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCcgcg                                                                                                                                                                                                                      <  1:1317215/238‑1 (MQ=255)

                     atatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTGCCGGGTCAC‑GGGACGC‑TGGCCGGGGATGATATCTATA‑TTTTCGG‑GAGGTTT‑TAGA‑TGGGGGAAATTGACCGCGAt                                                                                                                                                                                                                    >  1:558454/1‑239 (MQ=255)

                      tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:8147/238‑1 (MQ=255)

                      tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:34648/238‑1 (MQ=255)

                      tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:965225/238‑1 (MQ=255)

                      tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:1399234/238‑1 (MQ=255)

                      tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:1351963/238‑1 (MQ=255)

                      tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCGCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  2:37454/238‑1 (MQ=255)

                      tagcACCATTTGCTGGGGCGGGCGTGAAGAGCAGCATCTGTATTATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:1180708/235‑1 (MQ=255)

                          aCCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCGTGAGGCGCGTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑Gtt                                                                                                                                                                                                              <  2:345159/239‑1 (MQ=255)

                          aCCATGTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑Gtt                                                                                                                                                                                                              <  2:272172/239‑1 (MQ=255)

                            cATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCcgcg                                                                                                                                                                                                                      <  1:384219/230‑1 (MQ=255)

                                tACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAg                                                                                                                                                                                                        <  1:1115921/239‑1 (MQ=255)

                                  cTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTg                                                                                                                                                                                                      <  1:487741/239‑1 (MQ=255)

                                      ggcgggcgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGGAGGGGCAC‑GGGGGGC‑CGGGGGGGGGTGGTATCTTTT‑TTTTCGG‑GAGGTTT‑GAGAGTGGGGCAAATGGCCCGCGATTG‑Gtttt                                                                                                                                                                                                            >  2:384219/1‑230 (MQ=255)

                                      ggcgggcgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTAGTGCCGGGTCACGGGTACGC‑GGGGGAGGCAT‑ATATCTATA‑TTTCCGG‑ACGGTTT‑TTGA‑TGGCGCGAAATGTCCGCGGGCT‑Gtttttctgttgtgg                                                                                                                                                                                                  >  2:726845/1‑229 (MQ=255)

                                      ggcgggcgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTGGCAGATCCC‑GGGAAGC‑GTGCAGGGCCTGAGAACTATATTTGCCGG‑‑CGCTTT‑GGGATTGGGGGAAATT‑ACCGCGACCC‑GTTTTtgagtgaggc                                                                                                                                                                                                  >  2:1198784/1‑237 (MQ=255)

                                          ggcgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTTCAGGAGCAC‑GGGACCCGTGGCAGGGC‑TGATATTTCTT‑TTGCCGGCGGGTTTT‑GGGT‑GGGGGGAATTTCCCCGCGCGCT‑GTTTT‑CAGTgggggt                                                                                                                                                                                                 >  2:250384/1‑231 (MQ=255)

                                            cgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCACGCGATCT‑GTTTTGCAGTGAGCGTAATg                                                                                                                                                                                             <  2:841210/238‑1 (MQ=255)

                                                     cagcaTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCCGGGTCAC‑GGTACGC‑TGGCAGAGCATGGTATCTATA‑TTTCCGG‑GCGTTTT‑TGGA‑TGGGGAAAATTGCCCGCGCACT‑GGTTTGCAGTGGGCGGAATAGGCGGGaaaa                                                                                                                                                                                   >  1:151923/1‑237 (MQ=255)

                                                     cagcaTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGGTCAC‑GGGACGC‑TGGCAGAGCAAGATATCTATA‑TTGCCGG‑ACGTTTT‑GGGA‑TGGGGAAAATTTCCCGCGATCTGGTTTTGC‑GTGAGCGTAAAGCGCGGGgaga                                                                                                                                                                                   >  1:763400/1‑239 (MQ=255)

                                                        caTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGcc                                                                                                                                                                                                                          <  1:1145741/198‑1 (MQ=255)

                                                         aTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGAt                                                                                                                                                                                  <  1:1125832/236‑1 (MQ=255)

                                                                  aTCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGTAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCCGGGTCAC‑GGTACGC‑TGGCAGAGCATGATATTTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGGAAATTTCCCGCGATCT‑GTTTTTCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTTGc                                                                                                                                                                      >  1:636219/1‑239 (MQ=255)

                                                                  aTCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGCATTGCTGGAGCTC‑CGGACGC‑GGGGAGAGGAGGAAATCTCTA‑TGGCCGT‑GCGTTTT‑TTGA‑TGGCGGAAACTGACCTCGATAT‑GTCTTTATGTGAGGGCTATGAGCGGGGAGATGGCCCCTTTggt                                                                                                                                                                      >  2:1063842/1‑238 (MQ=255)

                                                                   tctcTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGAt                                                                                                                                                                                                                    <  1:1116626/193‑1 (MQ=255)

                                                                   tctcTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCg                                                                                                                                                                     <  1:33037/239‑1 (MQ=255)

                                                                    ctctGCGAGCTTGATGCGCTTTAGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGa                                                                                                                                                                    <  2:106068/239‑1 (MQ=255)

                                                                     tctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTGCAGGATCAA‑CGCGCAC‑TGGCGAGGCCAGATATCTTTA‑TTTCCGG‑GCGGTTT‑TTGG‑GGGCGGAAATTGTCCCCGACCT‑GTTTTTCCGTGATGGTGAAGCGCGGGAAgggaccccggttggcgtt                                                                                                                                                                   >  2:1141351/1‑222 (MQ=255)

                                                                      ctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGGGCAC‑GGTACGC‑CTGCAGAGCATGCTATATCTA‑TTTCCCG‑GCGGTTT‑TAGA‑TGGCGGCAAATGCCCCCGACCT‑GTTTTTCAGTGAGCGTTATGCGCGGGAAGATAGCCTGTTTTGCGagg                                                                                                                                                                  >  2:1079775/1‑237 (MQ=255)

                                                                      ctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGGACGC‑TGGCAGAGCATGATATCTATA‑TTTCCGG‑GCGTTTT‑TAGA‑TGGCGAAAATTGTCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGGAAATCGCCTGTTTTGCGAt                                                                                                                                                                   >  1:591905/1‑238 (MQ=255)

                                                                        gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCGGGAGCCC‑GGTAAGC‑CGGCCGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GGGA‑TGGCGAAAAATGACCCCGACCT‑GTTTTGTAGTGATGGGAAAGCGCGGGAAGAGTGCCTGTTTgtggttttg                                                                                                                                                                >  2:118921/1‑231 (MQ=255)

                                                                        gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCCGAGCATGGTATCTTTA‑TTTGCGG‑GCGGTTT‑GGGA‑TGGCGGAAAATTCCCGCGATCT‑GTTTTTTATTGAGCGGAATGCGCGGGGAGGATGCCTGTTTTGCGGTgtg                                                                                                                                                                >  1:1330566/1‑237 (MQ=255)

                                                                        gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGAGCAC‑GGGACGC‑CGGCCGAGCATGATATCTATT‑TTTCCGG‑GCGGTTT‑GGGG‑TGGGGGAAAATTCCCGCGGTCT‑GTTTTTCCGGTAGGCGGATGTGCGGGGAGATAGCCTGTTTGGCGGTggg                                                                                                                                                                >  2:1257250/1‑237 (MQ=255)

                                                                        gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGCTTGCAGGGTTGC‑GGGGCGC‑TGGCAGAGCATGCTATCTAGA‑TTTCCGG‑AGGGTTT‑TAGG‑TGGCGGAAAATGGCCGCGGTCT‑GGTTTGCAGTGAGCGGTATGGGCGGGGAGATTGACTGttttgggcgttg                                                                                                                                                                >  2:33037/1‑230 (MQ=255)

                                                                        gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTGCAGGATCAA‑GGGACGC‑TGGCAGAGCATGATATCTAAA‑TTGCCGG‑GCGGTTT‑GGGA‑TGGCGGAAATTGGCCCCGATCT‑GTTTTTTAGTGGGCGTAATTGGCGGGAAGATCGCCTTGTTGGCGGTGCg                                                                                                                                                                >  2:898141/1‑239 (MQ=255)

                                                                          gAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGt                                                                                                                                                               <  1:556086/238‑1 (MQ=255)

                                                                          gAGCTGGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGt                                                                                                                                                               <  1:1421872/238‑1 (MQ=255)

                                                                             cTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑CGGACGC‑CGGCCGAGCCTGATATCTATATTTGCCGG‑A‑GTTTT‑GGGG‑TGGGGGAAAATTTCCGCGGGCT‑GTTTTTCAGTGGGCGGCATACGCGGGGAGAATCCCCGTTTTGGGATGCGTTTg                                                                                                                                                            >  1:1191644/1‑238 (MQ=255)

                                                                              tttgggCGGTTTCGGTTAAAGATCCTGGTCGGCGAGTGGGGCCGGTGGTTGGACAACCGGAAGCGGGCTGGCGTGGGCGGACTGGCACCGTGTTAACGGCGGGATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGAAATGCGAGGGAAGATCGCCTGTGTGGTGATGCGTGTGCa                                                                                                                                                          <  2:932234/235‑1 (MQ=255)

                                                                              ttGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCa                                                                                                                                                          <  1:888845/239‑1 (MQ=255)

                                                                                       tttCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTg                                                                                                                                                 <  2:833388/238‑1 (MQ=255)

                                                                                          cGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑Gat                                                                                                                                            <  1:893820/239‑1 (MQ=255)

                                                                                             tGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATAGCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑Gatat                                                                                                                                          <  1:1063842/238‑1 (MQ=255)

                                                                                                       gggcgtGCAGGGGGGGCCGGTGGTTAAACAACCGGAGGCGGGCGGGCGTGGGCGTCGTGGCACCGTTTTAAGGGGGGTATTGAAGGCTCAG‑GGTAGGC‑TGGAAGAGCAGGATATCTTTT‑TTGCCGG‑ACGTTTG‑GAGA‑TGGCGAAATTTGCCCGGGATTT‑TTTTTGAAGTGAGCTTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATAAAAAAAACCCCg                                                                                                                               <  2:31919/234‑1 (MQ=255)

                                                                                                            tGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGttt                                                                                                                                                             <  1:1046880/206‑1 (MQ=255)

                                                                                                              cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGGATTGCAGGGTCAC‑GGGGCGC‑TGGCCGGGCCTGATATTTATA‑TTTCCCG‑GCGGTTT‑TAGA‑TGGCGGAAATTGCCCGCCATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATAGCCCTCTTGGTGATGTGTTTGCA‑TTTTTTTTA‑GATATAAAAAAAACCGCCCCTg                                                                                                                         >  2:507023/1‑238 (MQ=255)

                                                                                                                          gtgggTGAACACCCGGAAGCGGGCGGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑GGGCAGAGCATGATATCTATA‑TTGCCGG‑ACTTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCTGTGAGCGTAATGCGCGGAAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑AATATAAAAAACCCCGCCCCTGACA‑GGCGGgaag                                                                                                            <  1:1028387/238‑1 (MQ=255)

                                                                                                                               tGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTTGAGGATCAC‑GGGGAGC‑CGGCAGGGCAAGATATCTTTA‑TTGTCCG‑GCGTTTT‑TGGA‑TGGCGGAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAAAGCGCGGGAAGATTGGCTGTTTTGCGGTGCGTTTGCC‑TTTTTTTTAGGATATAAAAAAACCC‑CCCCCGTCACGGCGGgaagaagg                                                                                                        >  2:265009/1‑236 (MQ=255)

                                                                                                                                      ccGGGAGCGGGTGGGGGTGGGATTGCGGGCCCCGGTTTAACGGCGGGATTGCAGGACCCC‑GTTACGC‑TGGCAGAGCATGATATATATA‑TTGGCGG‑ACTTTTT‑GAGA‑GGGCAAAAATGCCCCGCGTTTT‑GTTTGGCAGTGAGCGTAGTGGGCGGGAAGAGCGCCTGTTGGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTa                                                                                                 <  2:1019655/237‑1 (MQ=255)

                                                                                                                                      ccGGAAGGGGGTGGGCGGGGGCGTACTGGCACCGTGTTAACGGCGGTTTTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATTTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGTTCT‑GTTTTGCAGTGAGCGTAATGCGGGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCGGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTaaa                                                                                               <  1:1347567/239‑1 (MQ=255)

                                                                                                                                                    ggggggcgctACTGCCCCCGGGTAAACGGGGTTATTGCAGGATCAC‑GTTCCGC‑TGGAAGGTCATGATATTTATT‑TTG‑CGG‑GCGTTTTGGGGA‑GGGCAAAAATTCCCCGCTATCT‑GTTTTGCAGTGGGGTAAAGGCGCGGAAAGACCGCCTTTTTGGCGATG‑GTTTGGATTTTATCGGA‑GATAAAAAAAACCCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTg                                                                                  <  2:151923/229‑1 (MQ=255)

                                                                                                                                                         ggggTACGGGCACCGTTTTAAGGGCGGTATTGGAGGATCCC‑GGTACGC‑GGGCAGAGCATGATATCTTTT‑TTGCCGG‑CCGTTTT‑AAGA‑GGGCAAAAATTGCCCGCGTTCT‑GTTTTGAAGTGAGCGTAATGCGGGGGAAGATCGCCTGTTTGGCGATGGGTTTGAA‑TTTATCTGA‑GATAAAAAAAACCCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAtt                                                                             <  2:588218/235‑1 (MQ=255)

                                                                                                                                                         ggCGTTGTGGCCCTGTGTAAAGGGGGGTATTGCAGGATCAC‑GGTACGC‑TGGAAGAGAATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCGGCGATCT‑GTTTTGAAGTGAGCGTAATGGGGGGGAAGATCGCTTGTTGGGCGAGGGGTTTGAA‑TTTATCTGA‑GATAAAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAtt                                                                             <  2:1330566/238‑1 (MQ=255)

                                                                                                                                                          gggTACTGGCACCGTTTTAACGGCGTTCTGGCAGGATCAC‑GGTCCGA‑TGGCAGAGCTTAATATCTATA‑TTGCCGG‑AGTTTTT‑GAGA‑GGGCGAAAATTGCCCGCGTTTT‑GTTTGGAAGTGAGCTTAAGGCGGGGGAAGATCGCTTGTTGGGGGATGCGTTGGCA‑TTTATCTGA‑GATAAAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCAttt                                                                            <  2:1191644/236‑1 (MQ=255)

                                                                                                                                                                                       tGCAGTATCAG‑GGTGCGG‑TGGCAGGGCATGATATCTATA‑TTGCCGG‑GCGTTTT‑GAGA‑TGGCGAAAATGGCCGGCGATCT‑GTTTGGCAGTGAGGTTAATGCGCGGGAAGATCGCCTGTTGGGCGAGGGGTTTGCA‑TTTATCTGA‑AATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGcc                                              <  2:615595/239‑1 (MQ=255)

                                                                                                                                                                                              tCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGG‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGGGGCATTTAGCTCTATGGCGTATCTGGCAAACCATGCCCGATGCg                                       >  1:1096712/1‑239 (MQ=255)

                                                                                                                                                                                              tCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCACCTAAAATGTTATTCAGGGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCg                                       >  2:501185/1‑239 (MQ=255)

                                                                                                                                                                                              tCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCg                                       >  1:670731/1‑239 (MQ=255)

                                                                                                                                                                                                aC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGAc                                     >  2:1387292/1‑239 (MQ=255)

                                                                                                                                                                                                 c‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGAAACTAAACTGGTATTCATTGGCATTTAGGTCTATGACGTATCTGGCAAACCATGCCCgtgtcgac                                     >  2:1416281/1‑231 (MQ=255)

                                                                                                                                                                                                   ggTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGAc                                     >  1:535047/1‑237 (MQ=255)

                                                                                                                                                                                                    gTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGcgcg                                                                                                                                                                                         >  1:1270298/1‑91 (MQ=255)

                                                                                                                                                                                                    gTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGcgcg                                                                                                                                                                                         <  2:1270298/91‑1 (MQ=255)

                                                                                                                                                                                                           tGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAAATAAACTGTTATTCAGTGGCAATTAGATCTATGACGAATCTGGCAAACCCTGTCCGATGCGACGCTGTcgcg                            >  2:218794/1‑239 (MQ=255)

                                                                                                                                                                                                             gCAGAGCATAATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑TTTTTGCAGTGAGGGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑AATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGt                           <  1:1257250/238‑1 (MQ=255)

                                                                                                                                                                                                              cAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAAAACGGACACTAAACTGTTATTCCGTGGGATATAGAACTCTGACGCTTATGGCAAACCCTGACACAGGCGACGCGGgcgccact                         >  2:1075494/1‑235 (MQ=255)

                                                                                                                                                                                                                gagCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCGTTTAGATCTATGACGTATCTGGCCAACCATGCCCGCTGCCACGCTGTCCGGTCTTa                       >  1:1266128/1‑239 (MQ=255)

                                                                                                                                                                                                                    aTGATATCTATA‑TTGCCGG‑CTGTTTT‑GAGA‑GGGCGAAAATTGCCCGGGATCT‑GTTTGGCATTGAGGGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATTTGA‑GATAAAAAAAACCCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCg                    <  2:763400/238‑1 (MQ=255)

                                                                                                                                                                                                                    aTGATATCTATA‑TGGCCGG‑ACGTTTT‑GAGA‑GGGCGAAAATTGCCCGCGATCT‑TTTTTGCAGTGGGCGTAATGCGCGGGAAGATCGCTTGTTTGGCGATGCTTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCg                    <  1:312234/238‑1 (MQ=255)

                                                                                                                                                                                                                             tctgttGCCGG‑AGGTTTG‑GAGG‑CGGCGAAATGTGCCCGCATTCG‑TTTTTGAAGTGGGCGTAATGGGCGGGAAGATGGCCTGTTTGGGGATTCGTTTGCA‑TTTAGCGGA‑AAAATAAAAAACCCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACaa           <  2:1266128/235‑1 (MQ=255)

                                                                                                                                                                                                                             ata‑tTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAACACCCGCCCCTGACA‑GGCGGGAAGAACGAGTACCAAACTGTTATTTAATTGCACTTAGACCTATGCCGGATCTGGCAAACCATGCCCGATCCCACGCTgaccgctcttactgcgcacaaaaa          >  2:200761/1‑216 (MQ=255)

                                                                                                                                                                                                                             ata‑tTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGGCGAATCTGGCAAACCATGCCCGATTCGACCCTGTCGCGTCTTATCGTGCCtcacaa          >  2:27543/1‑234 (MQ=255)

                                                                                                                                                                                                                             ata‑tTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACaaa          >  1:1016196/1‑239 (MQ=255)

                                                                                                                                                                                                                             ata‑tTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑CGCGGGGAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGAGGTATCTGGCAAACCATGCCCGATGCGACGCTGTTGCGTCTTATCGTGCCTACaaa          >  1:352773/1‑239 (MQ=255)

                                                                                                                                                                                                                               a‑tTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCCTTTAGATCTATGACGTATCTGGCAAAACATGCCCCATGCGACGCTGTCGCCTCTTTTCGTGCCTACaa           >  2:567502/1‑236 (MQ=255)

                                                                                                                                                                                                                                 ttGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAg       >  1:657106/1‑239 (MQ=255)

                                                                                                                                                                                                                                  tGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAg       >  2:271876/1‑238 (MQ=255)

                                                                                                                                                                                                                                  tGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGGATCTGGCCAACCATGCCCGATGCGACGCCGCCGCGTCTTATCGTTCCTACAAATAg       >  2:915932/1‑238 (MQ=255)

                                                                                                                                                                                                                                   gCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGt      >  1:1024428/1‑238 (MQ=255)

                                                                                                                                                                                                                                   gCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGt      >  1:1132921/1‑238 (MQ=255)

                                                                                                                                                                                                                                    ccGT‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGGTGCGACGCTGTCGCGTCTTATCCTGCCTACAAATAGTcc    >  1:334050/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAAcc                                                   >  1:566036/1‑192 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc    >  1:227646/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc    >  1:265806/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc    >  1:567742/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc    >  1:898089/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCGGTCGCGTCTTATCGTGCCTACAAATAGTcc    >  2:760032/1‑239 (MQ=255)

                                                                                                                                                                                                                                    ccGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAACTATGACGTATCTGGCAAAGCATGCCCGATGCGACGCTGTCTCGTCTTATCGTGCCTACaataaagc     >  1:1364049/1‑232 (MQ=255)

                                                                                                                                                                                                                                     cGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCg   >  2:730126/1‑239 (MQ=255)

                                                                                                                                                                                                                                     cGG‑ACGTTTG‑GAGA‑TGGCAAAAATGGCCCGCGATTT‑GTTTTGCAGAGAGCGTAAGGCGCGGAAAGATCGCTTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAACCCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCg   <  2:140046/239‑1 (MQ=255)

                                                                                                                                                                                                                                       g‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTCTCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATATTCCGa  >  1:1387659/1‑238 (MQ=255)

                                                                                                                                                                                                                                       g‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGa  >  2:73293/1‑238 (MQ=255)

                                                                                                                                                                                                                                       g‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAAACATGCCCGATGCGACGCTGTCGCGTCTTATCCTGCCTACAAATAGTCCGa  >  1:890562/1‑238 (MQ=255)

                                                                                                                                                                                                                                       g‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTAGCAAAACATGCCCCATGCGACGCTGCCGCGTCTTATCGTGCCTACAAATAATCCGa  >  2:469781/1‑238 (MQ=255)

TGGCGCGTAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCAC‑GGTACGC‑TGGCAGAGCATGATATCTATA‑TTGCCGG‑ACGTTTT‑GAGA‑TGGCGAAAATTGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCA‑TTTATCTGA‑GATATAAAAAAACCCGCCCCTGACA‑GGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGA  >  NC_000913/4026902‑4027367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`