Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,256,196 A→C 16.9% R90R (CGT→CGG)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,1960AC16.9% 201.7 / 4.2 101R90R (CGT→CGG) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (51/27);  new base C (4/17);  total (55/46)
Fisher's exact test for biased strand distribution p-value = 1.78e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

ACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTA  >  NC_000913/4255972‑4256434
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
aaaaacCTGTCGCCCCCGCGCCACAGTAAGGCAAAATATTTCTGTCCGCCGTTAAGCCGACGCA‑GGGGCGGGTTCTCTTCCCCTTTTTCCCCCCCCGG‑CGC‑GCGACAAAC‑AACCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGAAAATCGAGATAGTCGTGGAACAGCTAAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTAAA‑CACACGCGG‑CCCGCC‑Gtg                                                                                                                                                                                                                                  <  2:1098568/237‑1 (MQ=255)
  aaacCTGTCGCGCCCGAGCCACAGTACAGCAAAAAATTTCTGTACCCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTCACCCCCCGG‑CGC‑GCGCCCAAA‑CACCACCGACACTGGCACCATCTCCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑Gtgaa                                                                                                                                                                                                                                <  1:431109/239‑1 (MQ=255)
    aaCGGTCCCGACCGAGCCCAAGTACAGCGAAAAATTTCTGTACGCCGGTAAGCATACGCA‑GCGACGGGTTCACTTCGACTATTTCACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGAC‑Gtgaatg                                                                                                                                                                                                                              <  1:1351695/239‑1 (MQ=255)
                   gCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGTGTTTCCTTCTCCTTTTTCACCCCCCCG‑CGC‑GCGGCCCAA‑CATCACCGTCACTTGCACCAACAACCCCTCCCGAGTTTGTTTGTTCACGTGGCAAAAC‑CGAGAATCGAGCGACAGCTTAATAAAATCTTCCTTCTGCGT‑‑G‑‑TTTGTGGTGTA‑CAGCAACGG‑CCCGCC‑Gtgcaggtatgacacagccggc                                                                                                                                                                                                               >  2:825744/1‑220 (MQ=255)
                   ccacggataaatgaaaaaaTCTCTGTGCGCCGTAAAGCA‑ACCCACGCGGGGGGTCCTCTTCCCCTTTCTCCCCCCCGGG‑CGC‑GCTACAAAA‑CCTCCCCTCCACTGGCACCTTCTGCAAACCCAGTTTTGGTTTGAT‑ACGGGGCAAGTGGAGATGGTCGTGAAACAGCTTAATAGACTCTGCTTGCGGCTT‑‑G‑‑TAATAGGTGAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCgg                                                                                                                                                                                                                <  2:236862/224‑1 (MQ=255)
                         gTACAGCGAAAAATTTTTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCCCCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCCGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCCG‑CCCGCC‑GGGAATGAACACCTAACGCGGCCGGAGc                                                                                                                                                                                                         >  1:1092986/1‑239 (MQ=255)
                               cGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACCGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGGGcc                                                                                                                                                                                                    >  1:1384064/1‑238 (MQ=255)
                               cGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACCTCGCCTTTTTTACGCCCCGG‑CGC‑CCGGCCAAA‑AATCACCCACCCCGGCCCCCCCTTCACCTCCCGCATTGGGGTGCTGCCGGTGCAAA‑CGCGATAAGCGTGGAAAAGCTTTATACATACTTTTTTCCTGGG‑‑G‑‑TAATAAGGGAA‑CAACCGCGG‑TCCGCCAGGGAATGAAC‑CATAACGCGGcgagagcggtccg                                                                                                                                                                                                   >  2:473721/1‑227 (MQ=255)
                               cGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGGTCACTTCGCCTTTTTTACGCCCCCG‑CGC‑GCGACCAAA‑CATCACCGCCACAGGCCCCATCTGCACATCCAGATTTTGGTTGCT‑ACGGGGCAAAACGAGAAAGTCGGGGAAAAACTTAATAAAATCTTCTTTCGGGGG‑‑G‑‑TGAAAGGGGAA‑AAACCGCGG‑CCCGCC‑CGGAATGAAAACAAAAGGCGGCGGGAGGGTGcc                                                                                                                                                                                                    >  2:182815/1‑238 (MQ=255)
                               cGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGGTCACATTGCCTTTTTCACCCCCCCG‑GCC‑CCGGCCCAA‑CAACACCGACACCCGCCCCCTCCTCGCATCCCGATTTTGGTTTCT‑ACCGGGGAAAACGGGGTAGTCTGGGTAGCACCTAATTATCCCTTCTTTCGGCGT‑‑G‑‑TTAAAAGGGAA‑CACACGCGG‑CCCCCC‑GTGAATGAataccctacgctgcactcacgtggc                                                                                                                                                                                                    >  2:1188006/1‑217 (MQ=255)
                                 aaaaaTTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGG‑CGC‑GCGACCAAA‑CAACACCCCCACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGGGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTTTGCGT‑‑G‑‑TAATAGGTGGA‑CACACGCGG‑CCCGCC‑GTGAATAAACACATAGCGCGGCGGTAGCGTGcctcc                                                                                                                                                                                                 >  2:42451/1‑236 (MQ=255)
                                 aaaaaTTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACATCGCCCTTTTTACACCCCCG‑CGC‑GCGGACAAA‑CATCACCCACACTGGCCCCATCCTGACATCCCCATTTGGGTTGCT‑CCAACGCCAATCGAGATAGTCGTGGCACCACTTAAACGTCCATTCTTTCGGAGT‑‑G‑‑TAATCAGTGTA‑GCCACGCTG‑GCCGAC‑GGGTATAAAAACATAACGCGGAAgactgtgggcgac                                                                                                                                                                                                 >  2:1366904/1‑227 (MQ=255)
                                    aaTTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTTACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACCGGCACCATCTGCACATCCAGATTTTGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCTGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATTAACACATAGCGCGGCAGTAGCGGGCCGCCGAt                                                                                                                                                                                              >  1:78229/1‑239 (MQ=255)
                                          tGTCCGCCTTAAAGCATCCGCG‑GGGGGGGGTTCACTCCGCTTTTTCCCCGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAg                                                                                                                                                                                        <  1:565251/239‑1 (MQ=255)
                                          tGTACCCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTCCCGCCCCGG‑CGC‑GCGACAAAA‑CATCCCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAg                                                                                                                                                                                        <  1:614665/239‑1 (MQ=255)
                                              cGCCGTTAAGCAGACGCA‑GCGGC‑GGTTCCCTTTCCCTTTTTTCCCCCCCGG‑GGCGGCGACAAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑AGGGGGCAAAGAGGGATAGTCGGGGAACAGCTTAATAGACTCTTCTTGCGGCGT‑‑G‑‑TAATAGGGGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGc                                                                                                                                                                                    <  2:343366/239‑1 (MQ=255)
                                                  gTTAAGCACCCGCG‑GCGGGGGATCCACTTCGCCTTTTCCA‑GCCCCGGCCGG‑GCGCCCAAA‑CCTCCCCGACTCTGGCACCATCTGCACATCCAGTTTTGGGTTGCT‑GCGGTGGAAAGCGAGATGGTCGGGAAACAGCTTAATAGCCTCTTCTTTCGGGGT‑‑G‑‑AAATAGGTGAA‑CCCCCGGGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCTGTCGATTTCCAGCGGCTCTa                                                                                                                                                                                <  2:1201316/239‑1 (MQ=255)
                                                    aaaGCACCCGCC‑GCGGCGGGTCCCCTCCGCCTTTTCCCCCCCCCGG‑CCC‑GCGACAAAA‑CACCCCCGACACTGGCACCATCTGCACATCCAGTTTTGGTTTGCT‑ACGGTGCAAATGGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGGGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTaa                                                                                                                                                                               <  2:182008/237‑1 (MQ=255)
                                                           aCGCA‑GCGGCGGGTTCACTTCGCCTTTTTCACCCCCCCG‑CGC‑GCGACCAAA‑AATCCCCCACACTGGCACCCTCTGCCCATCCCGATTTGGGGTGCT‑ACGGTGCAAAACGAGGTAGTCGGGGAACAGCTTAATAGAATCTTCTTTCTGCGT‑‑G‑‑TTATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAAAACATAGCGCGGCAGTAGCGTGCCGGCGATTTTCCGCGGCTCTCACGGGTCAg                                                                                                                                                                       >  2:515186/1‑239 (MQ=255)
                                                           aCGCA‑GCGGCGGGGTCACTTCGCCTTTTTTACGCCCCGG‑CGC‑GCGGCCAAA‑CATCACCGCCACCGGCACCCTCTTCACCTCCCGATTTTGGTTGCT‑ACGGGGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCCGCGTGGG‑‑TAATAGGTGAA‑AACCCGCCG‑CCCCCC‑GCGAATGAACACATAGCGCCGCCGTAGCGGGCCGTCGGTTTccccgcgctccaaccggtc                                                                                                                                                                         >  2:637320/1‑222 (MQ=255)
                                                                  cggcggGTTCACTTCGCCTTTTTCACGCCCCCG‑CGC‑GCGACCAAA‑CATCACCCACACCGGCACCATCTGCCCATCCCGATTTTGGGTGCT‑ACGGTGCAAATCGAGATAATCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGG‑‑G‑‑TAATAAGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAAAACATA‑‑GCGGCAGTGGCGTGCCGCTCATTTCCAGCGGGCTTAACACGTCAGGCaaaacc                                                                                                                                                               >  2:602506/1‑235 (MQ=255)
                                                                    gcggGTTCACTTCGCCTTTTTCCCGCCCCGG‑CGC‑GCGACAAAA‑CACCACCGACACTGGCCCCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAAAAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                                                                                                                                                <  1:620058/238‑1 (MQ=255)
                                                                      gtgtccccTTCCCCTTTTCCCCCCCCCGG‑CCC‑CCACCAAAA‑CATCCCCGACACTGCCACCATCTGCACACCCAGATTTGGTTTGTT‑ACGGTGAAAATCGAGATAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAt                                                                                                                                                              <  1:42451/231‑1 (MQ=255)
                                                                      gggTCCCCTCCGCCTTTTCCCCCCCCCGG‑CGC‑GC‑ACCAAACCACCCCCGACACTGGCACCATCTGCACATCCAGATTGGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGAAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAt                                                                                                                                                              <  1:295209/238‑1 (MQ=255)
                                                                                  ccTTTTTCACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTg                                                                                                                                                 >  1:430018/1‑239 (MQ=255)
                                                                                    tttcacACGCCCGGG‑CGC‑GCGCACAAA‑CATCACCGACACGGGATCCATCGGCACACCAAGTTTGGGGTTGCT‑CGGGGGAAAAGCGAGAGAGTCGGGAAACAGTTTAATAGCCTCTTCTTCGGGGGT‑‑G‑‑AAAGGGGGGAA‑CACGCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTggg                                                                                                                                               <  2:666874/234‑1 (MQ=255)
                                                                                                               aa‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCtt                                                                                                                       >  1:542698/1‑238 (MQ=255)
                                                                                                                       ccGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTg                                                                                                               >  2:932370/1‑239 (MQ=255)
                                                                                                                       ccGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTg                                                                                                               >  1:743306/1‑239 (MQ=255)
                                                                                                                             cTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGt                                                                                                         >  1:420923/1‑239 (MQ=255)
                                                                                                                                gccccATCTGCACATCCAGTTTGGGGTTGCT‑ACGGGGAAAATCGAGATAGTCGTGGAACAGCTAAATAGCCTCTTCTTTCGGCTT‑‑G‑‑TAATGGGTAAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAt                                                                                                                                                              <  2:84856/180‑1 (MQ=255)
                                                                                                                                 cgccATCGGCACATCCAGATTTGGTTTGCT‑ACGGTGCAAAGCGGAATAGTCGTGGAACACCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAC‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      <  1:1020265/236‑1 (MQ=255)
                                                                                                                                 ccccATCTGAATACCTAGATTGGGGTTGCT‑ACGGTACAAGTAGAAATAGTCGGGAAACAGGTTAATAGACTCTTCTGTGGGTGT‑‑G‑‑AAATAGGTGAC‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      <  2:100574/236‑1 (MQ=255)
                                                                                                                                 cACCATCGGCCCATCCAGTTTTGTGTTGCT‑CCGGTGCAAATCGAGATAGTCGTGAAACACCTTAATAGCCTCTTCTTTCGGCGT‑‑GTATAATAGGTGAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaa                                                                                                       <  1:637320/239‑1 (MQ=255)
                                                                                                                                 cACCATCGGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTGGGGGAACAGCTTAATAGACTCTTCTTGCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      <  1:1188006/238‑1 (MQ=255)
                                                                                                                                      tCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTAc                                                                                                >  1:74501/1‑239 (MQ=255)
                                                                                                                                              tCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAACACGTACATAATTgg                                                                                        >  1:671489/1‑239 (MQ=255)
                                                                                                                                                gataTTTGGGTTGGT‑GGGGTGAAAGTCGAGATGGTCGTGGAAATGCTAAAAAGCCTCTTCTGTGGGCGT‑‑G‑‑TATTAGGTGAC‑CAGCCGCGA‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                                                                      <  2:1296504/236‑1 (MQ=255)
                                                                                                                                                cAGTTTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAC‑CCCACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                                                                      <  1:182815/239‑1 (MQ=255)
                                                                                                                                                cAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CCCACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                                                                      <  1:976675/239‑1 (MQ=255)
                                                                                                                                                cAGATTTGGGTTCCT‑ACGGTGCAAATCGAGATACTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                                                                      <  1:1022105/239‑1 (MQ=255)
                                                                                                                                                  gATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACga                                                                                    >  1:1166413/1‑239 (MQ=255)
                                                                                                                                                  gATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACga                                                                                    >  1:513617/1‑239 (MQ=255)
                                                                                                                                                    ttggggTTGCT‑AGGGTGCAAATCGAGATAGTCGGGGACCAGCTTAATAGCCTCTTCTTCGGGCGT‑‑G‑‑AAATAGGTGAC‑CACGCGCGC‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACgaga                                                                                  <  1:515186/236‑1 (MQ=255)
                                                                                                                                                         gTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGAGTCAGGCAAGTCATGTGCCAGACACTGAGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTa                                                                              >  1:1181425/1‑238 (MQ=255)
                                                                                                                                                           tGCT‑ACGGTGCAAACCGAAATAGTCGTGAAACAGCTTAATAGCCTCTTCTTTCGGCGT‑‑G‑‑AAATAGGTGAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATcc                                                                           <  1:784459/239‑1 (MQ=255)
                                                                                                                                                            tgctcgggTGCAAATCGAGATGGTGGGGGAACGCATAAATAGCCTCTTTTTGCGGGGT‑‑G‑‑AAATGGGTAAA‑CCCCCGCGC‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATcc                                                                           <  2:643947/233‑1 (MQ=255)
                                                                                                                                                                   gggCAAAGCGGGATGGTCGGGAAACGCCATAATAGTCTCTCTTTTCGGCTT‑‑G‑‑TAATAGGTGAA‑CCCCCGGGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGcccc                                                                     <  2:642983/236‑1 (MQ=255)
                                                                                                                                                                   gTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCa                                                                    >  2:486764/1‑239 (MQ=255)
                                                                                                                                                                       aagagaggatgttggtgagaCAGCAAAATACACTCTTTTGTCGGCGT‑‑G‑‑TAAAAGGTGAA‑CACACGCCG‑CCCGCT‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTc                                                                <  1:819369/220‑1 (MQ=255)
                                                                                                                                                                       aactCGATATAGTCGTGAACCCGCTTAATAGCCTCTCTTTGCGGCGT‑‑G‑‑TAATAGGTGAA‑CCCACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTc                                                                <  1:1172363/236‑1 (MQ=255)
                                                                                                                                                                        aaTGGATATAGTGGGGGAACAGCTAAATAACGTCTTTTTGCGGCGT‑‑G‑‑TAATAGGTGAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTcc                                                               <  2:78229/239‑1 (MQ=255)
                                                                                                                                                                        aaTCGCGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCCGCGGCTCTAACGGTTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCCAACCTGCTTTCGCGTTGTACGGAAAAACGTACATAATTGGACGAGAGGTATCGAGCCACAGTTcc                                                               >  2:809087/1‑239 (MQ=255)
                                                                                                                                                                        aaTCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTcc                                                               >  1:1188337/1‑239 (MQ=255)
                                                                                                                                                                                   tCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAACTCTGCTTTCGAGTTGTACGGTAAAACGGACATAATTGGACGAGAGGTCTCCAGCCCCAGTTCCGGGGCAggt                                                      >  2:177922/1‑236 (MQ=255)
                                                                                                                                                                                   tCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGCCGAGAGGTATCCCGCCCCAGTTACGGGGCAGGa                                                      >  2:1339839/1‑237 (MQ=255)
                                                                                                                                                                                   tCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                      >  1:309812/1‑237 (MQ=255)
                                                                                                                                                                                   tCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                      >  1:950605/1‑237 (MQ=255)
                                                                                                                                                                                   tCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGa                                                      >  1:813440/1‑237 (MQ=255)
                                                                                                                                                                                     gTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                                                                                                                                                >  2:1305841/1‑129 (MQ=255)
                                                                                                                                                                                     gTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                                                                                                                                                <  1:1305841/129‑1 (MQ=255)
                                                                                                                                                                                       ggAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGc                                                 <  1:473721/238‑1 (MQ=255)
                                                                                                                                                                                            aGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAAt                                           >  1:1422611/1‑239 (MQ=255)
                                                                                                                                                                                              cTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAg                                         >  1:740461/1‑239 (MQ=255)
                                                                                                                                                                                              cTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GGGACTGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACCCTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACAGAATTGGACGAAAGGTATCCAGCCCCATTTACGTGGCAGGAACGGCCGGAATAg                                         >  2:631526/1‑239 (MQ=255)
                                                                                                                                                                                                aaaTAGACTCTTCTTCCGGCGT‑‑G‑‑TAATGGGTGAC‑CCCACGCGG‑ACCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGAc                                       <  1:711876/238‑1 (MQ=255)
                                                                                                                                                                                                 aaTAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGTGGCAGGATCTGCCGGAATAGACt                                      >  2:72501/1‑239 (MQ=255)
                                                                                                                                                                                                     acctctTCTTTCGGCGT‑‑T‑‑GAATAGGTGAA‑CCCCCGCGC‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCt                                  <  1:713594/237‑1 (MQ=255)
                                                                                                                                                                                                      aCTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtt                                 >  2:1341192/1‑239 (MQ=255)
                                                                                                                                                                                                      aCTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtt                                 >  1:591841/1‑239 (MQ=255)
                                                                                                                                                                                                        tcttctTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      >  2:460643/1‑168 (MQ=255)
                                                                                                                                                                                                        tcttctTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtttt                               >  1:1205879/1‑239 (MQ=255)
                                                                                                                                                                                                        tcttctTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtttt                               >  1:1218817/1‑239 (MQ=255)
                                                                                                                                                                                                        tcttctTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCCCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAAAGTACATAATTGGACGCGAGGTATCCAGACCCAGTTCCGGGGCAGGATCTGCCGGAACAGACTTGGtttt                               >  2:1023571/1‑239 (MQ=255)
                                                                                                                                                                                                        tcttctTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGACATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCtttt                               >  2:934792/1‑239 (MQ=255)
                                                                                                                                                                                                        tcttctTTCGGCGT‑‑G‑‑AAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      <  1:460643/168‑1 (MQ=255)
                                                                                                                                                                                                         cttcttTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCttttt                              >  2:415053/1‑239 (MQ=255)
                                                                                                                                                                                                          ttcttTCGGCGT‑‑T‑‑AAATAGGTGAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTa                             <  1:229985/239‑1 (MQ=255)
                                                                                                                                                                                                             tttGGGCGT‑‑G‑‑AAATAGGTAAA‑CACCCGCGG‑CCCGCC‑GTGAATGAACACATA‑‑GCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAgg                        <  1:602506/239‑1 (MQ=255)
                                                                                                                                                                                                               gcGGCGT‑‑G‑‑‑AATACGTGAACCCCACGCGC‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAgg                        <  2:743306/238‑1 (MQ=255)
                                                                                                                                                                                                                    gt‑‑g‑‑tAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCt                   >  1:944068/1‑239 (MQ=255)
                                                                                                                                                                                                                    cgggttgagagtgctGAA‑CCCACGGGG‑CCCGCC‑GTTAATGAACACATAGCGCGGCAGTAGCGTGCCGTCTATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCTGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAgg                        <  1:498623/224‑1 (MQ=255)
                                                                                                                                                                                                                        g‑‑tAATAGGTAAA‑CCCCCCCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                 <  1:920365/239‑1 (MQ=255)
                                                                                                                                                                                                                         tttAATAGGTGAA‑CACCCGGCG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCt                   <  1:1195465/236‑1 (MQ=255)
                                                                                                                                                                                                                         agaaagagggaaa‑CACCCGGGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCt                   <  2:1205879/227‑1 (MQ=255)
                                                                                                                                                                                                                          ttAATAGGTGAC‑CCCCCGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTa                 <  1:934252/238‑1 (MQ=255)
                                                                                                                                                                                                                          ggaaatgggcga‑cacgcGCGGCCCCGGC‑GTGAATGACCGCATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCt                   <  2:1080827/227‑1 (MQ=255)
                                                                                                                                                                                                                               aGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGt            <  1:987645/239‑1 (MQ=255)
                                                                                                                                                                                                                                 gTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaa          >  1:895887/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTaa          >  1:52305/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gTGAA‑CACACGCGG‑CACGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGAAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCGGGATCTGCCGGAATAGACTTGCTTTTTACCTGGCTGCTTAATGGCaa          >  1:920213/1‑239 (MQ=255)
                                                                                                                                                                                                                                    aa‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      <  1:1192192/144‑1 (MQ=255)
                                                                                                                                                                                                                                    aa‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                                                                      >  2:1192192/1‑144 (MQ=255)
                                                                                                                                                                                                                                    aa‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAAt        >  2:701320/1‑238 (MQ=255)
                                                                                                                                                                                                                                     a‑cacacGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTc      >  1:823722/1‑239 (MQ=255)
                                                                                                                                                                                                                                     a‑caaacGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTc      >  2:836249/1‑239 (MQ=255)
                                                                                                                                                                                                                                       cacacGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCt                                  >  2:1128823/1‑210 (MQ=255)
                                                                                                                                                                                                                                       cacacGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCt                                  <  1:1128823/210‑1 (MQ=255)
                                                                                                                                                                                                                                       cacacGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCa     >  2:847125/1‑239 (MQ=255)
                                                                                                                                                                                                                                          acGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTa  >  1:83451/1‑239 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                
ACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAATTTCTGTACGCCGTTAAGCATACGCA‑GCGGCGGGTTCACTTCGCCTTTTTCACGCCCCGG‑CGC‑GCGACCAAA‑CATCACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGT‑‑G‑‑TAATAGGTGAA‑CACACGCGG‑CCCGCC‑GTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATCTGCCGGAATAGACTTGCTTTTTACCAGGATGCTTAATGGTAAATTCAGTA  >  NC_000913/4255972‑4256434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑