Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 45 [0] [1] 47 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)44 (0.920) 37/266 0.2 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258542‑258826
                                                                                                                                              |                                                                                                                                              
tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                <  2:81218‑M2/1‑1 (MQ=255)
tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                                >  1:19607‑M2/143‑143 (MQ=255)
      aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatgttcgtgaaggtaAGTGaaa                                                                                                                                          >  1:442331‑M2/137‑143 (MQ=255)
             ctaacttttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                   <  2:268455‑M2/14‑1 (MQ=255)
                  tgtcgcgactgtggtaacacagttgcatcacaacaggagatagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaacttaccgcactaggcccctacattcgttaaggtaAGTGCAAAGATAATCGAtt                                                                                                                              <  1:316422‑M2/19‑1 (MQ=255)
                   tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                             <  2:237161‑M2/20‑1 (MQ=255)
                   tctgccactgtcgtaaaacacttgcatcacaacaggagatagcaaggacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactcggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                             <  2:266230‑M2/20‑1 (MQ=255)
                       ccaatttggtaaaacagttgcatcatacatcaacacatcaaagacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCtttt                                                                                                                         >  1:728866‑M2/120‑143 (MQ=255)
                        caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        >  2:709758‑M2/119‑143 (MQ=255)
                        caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                        >  1:420440‑M2/119‑143 (MQ=255)
                         aatctgctaaaacagtcgcctcacagcaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcataaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                       <  1:27206‑M2/26‑1 (MQ=255)
                          atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTGTTTTTCg                                                                                                                      >  1:672749‑M2/117‑143 (MQ=255)
                           tttggtacaagagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                     <  1:96716‑M2/28‑1 (MQ=255)
                             tggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcattaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                   <  1:268883‑M2/30‑1 (MQ=255)
                                 aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt                                                                                                               <  2:819084‑M2/34‑1 (MQ=255)
                                   aacaggtgcagcacaccaggaaatagcaatgacgttaccaagtggacacccgaagagcagattgttcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                             <  1:608507‑M2/36‑1 (MQ=255)
                                     cagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                           <  2:135814‑M2/38‑1 (MQ=255)
                                               acaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                                                                 >  2:707692‑M2/96‑143 (MQ=255)
                                                ccacaggggatagcgaggacgtgaccgagcggacacccgaagagcagattgatcaaaaaatttaccgcgctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATGCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                                <  1:258667‑M2/49‑1 (MQ=255)
                                                caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                                >  2:180865‑M2/95‑143 (MQ=255)
                                                   caggggatagcaatgacgtgaccgagtggacacccgaagagcagattgatcaaaaaatgtaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTCTGCGTAAACGt                                                                                             <  1:679160‑M2/52‑1 (MQ=255)
                                                      gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTGTGCGTCAACGTGaa                                                                                          >  2:335415‑M2/89‑143 (MQ=255)
                                                      gagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGGTACAACGTGaa                                                                                          >  1:627641‑M2/89‑143 (MQ=255)
                                                       agatagcaatgaccataccgagggcacacacgaagagcagattgacaacaaaatttaccgcactaagcccgtctattcgtgaaggtaAGTGCAAAGATAATCGATCCTTGTTCGATTGTCTGGCTGTATGCGTCAACGTgaat                                                                                         <  2:80878‑M2/56‑2 (MQ=255)
                                                          tagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGACacca                                                                                      >  1:882809‑M2/85‑142 (MQ=255)
                                                                    gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGACCAAAGATAGACGAGTCTTTTTCGATTGTCTGGCTGTATGCGTCAAAGGGAAACCGGCACCGGAAg                                                                            >  1:102522‑M2/75‑143 (MQ=255)
                                                                      taccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                                                                          >  2:516209‑M2/73‑143 (MQ=255)
                                                                       agcgagtgtacacccgtggggcagattgatcaaacattttaccggactaggcgcgtctattggtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                                                                         <  1:266945‑M2/72‑1 (MQ=255)
                                                                               gacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                 >  2:171589‑M2/64‑143 (MQ=255)
                                                                                 cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                               <  2:742162‑M2/82‑1 (MQ=255)
                                                                                 cacccgaagagcagattgatcaaaaaatttaccgaactaggcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                               <  1:763868‑M2/82‑1 (MQ=255)
                                                                                      ggagaggagatggagcaaaaaatttaccgccctaggcccttatattcgtgaaggtaAGTGCAAAGATATTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCt                                                          <  2:425210‑M2/87‑1 (MQ=255)
                                                                                         gagcagattgatcaaaaaattttccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                       <  2:25369‑M2/90‑1 (MQ=255)
                                                                                         gagcagagtgatgcaaaaatttaccgcactgggcccgtatattcgtggaggtaAGTGCAAAGATAATCGAGTGTGTGGCGATGGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggt                                                       <  1:820809‑M2/90‑1 (MQ=255)
                                                                                            cagattgatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGACGTGCGTGAATTCTGGGGCTGGcgga                                                    >  1:377272‑M2/51‑143 (MQ=255)
                                                                                             agattgatcaaaaaatggaccgcactaggcccggagatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGcggat                                                   <  2:224074‑M2/94‑1 (MQ=255)
                                                                                                  gatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGc                                              <  2:728866‑M2/99‑1 (MQ=255)
                                                                                                    tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACAGCCACCGGAGGTGCGTGAATTCTGGGGGTGTTGGATGGAGGTg                                            >  2:450255‑M2/43‑143 (MQ=255)
                                                                                                        aaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                        <  1:516209‑M2/105‑1 (MQ=255)
                                                                                                         aaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgc                                       >  2:658550‑M2/38‑143 (MQ=255)
                                                                                                                ccgcactaggcccgtatatgggtgaaggtaAGTGCAAAGATAATCGGTGTTTGTTCGATTGTCTGGCTGTATGCGTGAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGTTGGAAGCGCAGGaa                                <  1:24478‑M2/113‑1 (MQ=255)
                                                                                                                           ccgtattttcgtgaaggtaAGTGCAAAGATAATCGAGTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                     <  1:152138‑M2/124‑1 (MQ=255)
                                                                                                                               tttttcgtgtaggttAGTGCAAAGATAATCGATTCTTTTTCGGTTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                 <  1:17215‑M2/128‑1 (MQ=255)
                                                                                                                                 attcgtgaaggtaAGTGCAAATATATTCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAg               <  1:722394‑M2/130‑1 (MQ=255)
                                                                                                                                 attcgtGAAGGTAAGTGCAAAGATAATCGCTTCTTTTTCGATTGTCTGGCTGTATGCGCCAACGTGACACCGGCACCGGAAGTGCGTGCATTCTGGGGCCGCTCGCTGGCGCCCGACGCGCACCAATCCCGTTTTACCtcccg               >  1:1192/6‑139 (MQ=255)
                                                                                                                                      tgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCCGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTGCAGTTAcc          <  2:632888‑M2/135‑1 (MQ=255)
                                                                                                                                              aGTGCAGAGATAATCGATCATTTTTCGATTGTCGGGCTGTATGCGTCGACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGCATCCCGTTTTACCTACAGTTACCAGTTTGGt  <  1:385214/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGT  >  NC_000913/258542‑258826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: