Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	59 [0]	[0] 60	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	57 (1.160)	48/274	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300557‑1300840                                                                                                                                              |                                                                                                                                               attacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaat                                                                                                                                               <  2:421791‑M2/2‑1 (MQ=255)  ttccagttagttataaggatttcctgaaccgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagattacttgagatgatattattaattccactgcctttggtagaggaaagtgctaaata                                                                                                                                              <  1:351656‑M2/3‑1 (MQ=255)     cagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacctcataatcgtgggcctgctgaagaataattgaaatgatattattaattccactgcctttggtcgaggaaagtgctaaataata                                                                                                                                           >  1:822210‑M2/138‑143 (MQ=255)      agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                          >  1:294301‑M2/137‑143 (MQ=255)       tttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataat                                                                                                                                         <  1:128120‑M2/8‑1 (MQ=255)        ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcttttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  2:790740‑M2/135‑143 (MQ=255)        ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  1:142166‑M2/135‑143 (MQ=255)        ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                        >  2:436071‑M2/135‑143 (MQ=255)          agttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccctttgtagaggaaagtgctaaATAATAATCaa                                                                                                                                      >  1:20879‑M2/133‑143 (MQ=255)              ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatactgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                  <  1:168811‑M2/15‑1 (MQ=255)               taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATTAATTGtt                                                                                                                                 >  2:366872‑M2/128‑143 (MQ=255)               taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgagcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                                 >  1:120873‑M2/128‑143 (MQ=255)                  ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                              <  2:351440‑M2/19‑1 (MQ=255)                       tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                         <  2:427747‑M2/24‑1 (MQ=255)                       tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                         <  1:429754‑M2/24‑1 (MQ=255)                       tccctaactgcttcccctgcacagcatgttattttcgccacatcataatacttagcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                         <  1:144258‑M2/24‑1 (MQ=255)                              ctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgacatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAtt                                                                                                                  >  1:168117‑M2/113‑143 (MQ=255)                                 cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               <  2:207753‑M2/34‑1 (MQ=255)                                       ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattacttccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTCCATTTCACTACTg                                                                                                         >  1:370967‑M2/104‑143 (MQ=255)                                             atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattacttccactgcctttggtagaggaaagtgctacCTGCTCGCCGAGTTGTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                   <  1:537039‑M2/46‑1 (MQ=255)                                             atcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                   >  2:23163‑M2/98‑143 (MQ=255)                                                  gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              >  2:752030‑M2/93‑143 (MQ=255)                                                   ttattttcgccacttcagaatcctgggcttgatgaagaataattgaaatgatattatgaattccactgcctttggtataggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                             <  1:226830‑M2/52‑1 (MQ=255)                                                     attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTTTGCATTTCACTACTGGAACTGTAATCAGa                                                                                           >  1:636900‑M2/90‑143 (MQ=255)                                                       tttcgtcacatcataatcctgggcttgatgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                         <  2:639416‑M2/56‑1 (MQ=255)                                                          cggtatatcataattctgggcttgatgaagattaattgaaatgatattatgaattccagtgcgtttggtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                      <  1:486705‑M2/59‑1 (MQ=255)                                                           gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                     >  2:512904‑M2/84‑143 (MQ=255)                                                            ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccgttggtagaggaaagtgctaaACAATAATCAATTGTTAAATTATGGTGCATTTCACTACTGGAACTGTAATCAAAAAAGATa                                                                                    >  1:436482‑M2/83‑143 (MQ=255)                                                              acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                  <  2:674812‑M2/63‑1 (MQ=255)                                                              acatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                  >  1:289569‑M2/81‑143 (MQ=255)                                                                 tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagcggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                               >  2:52876‑M2/78‑143 (MQ=255)                                                                 tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCACTGTAAGCAGAAAAGATAGACAt                                                                               >  1:4667‑M2/78‑143 (MQ=255)                                                                 tcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACAt                                                                               >  2:504533‑M2/78‑143 (MQ=255)                                                                   ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             >  1:370746‑M2/76‑143 (MQ=255)                                                                   ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             <  2:384955‑M2/68‑1 (MQ=255)                                                                    taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                            >  2:251118‑M2/75‑143 (MQ=255)                                                                     aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaCTAATAATCAATTGTTAAATTGTTGTGCATCTCACTACTGGAACTGTCATCCGAACCGCTAGACATGCtt                                                                           >  2:14200‑M2/74‑143 (MQ=255)                                                                        cctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                        <  2:294301‑M2/73‑1 (MQ=255)                                                                                  ctgaagaataattgaaatgatattattaatttcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                              <  1:460980‑M2/83‑1 (MQ=255)                                                                                   tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCActaat                                                             >  1:15237‑M2/60‑141 (MQ=255)                                                                                       gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTGTttgat                                                         >  2:48315‑M2/56‑143 (MQ=255)                                                                                            attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                    >  2:256648‑M2/51‑143 (MQ=255)                                                                                               gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                                 >  2:814457‑M2/48‑143 (MQ=255)                                                                                                   tgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                             <  2:142166‑M2/100‑1 (MQ=255)                                                                                                    gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATCGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                            >  1:182973‑M2/43‑143 (MQ=255)                                                                                                    gatattattaattccactgcctttggtagagaaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                            >  2:209506‑M2/43‑143 (MQ=255)                                                                                                      tattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATAAGAATAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgt                                          >  2:605065‑M2/41‑143 (MQ=255)                                                                                                          attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCACTCTCTATTTGCTTGACTTGAAAGAtgtttgt                                      >  2:40365‑M2/37‑143 (MQ=255)                                                                                                                        ctttggtagaggaaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                        <  2:20879‑M2/121‑1 (MQ=255)                                                                                                                        ccttggtagaggaaagtgctaaATAATAATCAATTGTTCAATTATTGTGCATTTCACTACTGGAACTGTAATCACAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                        <  2:706591‑M2/121‑1 (MQ=255)                                                                                                                          ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAACGATAGACATGCTTAGCCAGTCTCTATTTGATTGAATTGAAAGCTGTTTGTTAAGCCAGGGAtgcga                      >  2:390774‑M2/21‑141 (MQ=255)                                                                                                                              tagaggaaagtgctaaCTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGCTGTTTGTTAAGGCATGGATGCAAGCta                  <  1:339119‑M2/127‑1 (MQ=255)                                                                                                                               agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                 <  1:202436‑M2/128‑1 (MQ=255)                                                                                                                                gaggaaagtgcgaaATAACAAGCAAGGGTTAACGTATTGTGCCTTGCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtata                <  2:493808‑M2/129‑1 (MQ=255)                                                                                                                                   gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt             <  2:423807‑M2/132‑1 (MQ=255)                                                                                                                                      agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt          >  2:271133‑M2/9‑143 (MQ=255)                                                                                                                                      agtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCt          <  1:256648‑M2/135‑1 (MQ=255)                                                                                                                                           taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAGGCTATAGATTCTGATAc     >  1:687474‑M2/4‑143 (MQ=255)                                                                                                                                            aaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACg    >  1:837367‑M2/3‑143 (MQ=255)                                                                                                                                              ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  2:168808/143‑1 (MQ=255)                                                                                                                                              |                                                                                                                                               ATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300557‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑