Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA98.0% 145.3 / NA 53P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (19/31);  total (21/32)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184404‑4184678
                                                                                                                                          |                                                                                                                                        
tGGCGGTGAAACGCCGTATCCAGCCTGGTGACAAGATGGCCGGTCGTCACGGCAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTg                                                                                                                                      <  2:119273/143‑1 (MQ=255)
      ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                    <  2:83855/139‑1 (MQ=255)
      ttAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTggg                                                                                                                                    >  1:83855/1‑139 (MQ=255)
        gatctggcttactaGCCTTGTTATAAGTTGGACGTTCTTCACGGTAACATGGGTGTAATTTCTAAGTGCCGGCAGATCTAAGATATGCCTTACGATGAAAACGTTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAcc                                                                                                                              <  1:605670/130‑1 (MQ=255)
          aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGGACGAGCTGGGCGTACCgg                                                                                                                            >  1:483089/1‑142 (MQ=255)
          aCGCCGTATCCAGCCTGGTGACAACATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAGCCCGAACGACGATCTGTCTTATGATGAAAGCAGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                            <  2:93868/143‑1 (MQ=255)
                tATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                      <  2:193953/143‑1 (MQ=255)
                 ttctcggatggtggAAAGATGGCCGGTCGTCCCGGTAACAAGCGTGTAATTTCTAAGATCAACCCCATCTAAGTTATGCCTTACGATGAAAACGGTCCGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                     <  1:322530/135‑1 (MQ=255)
                 aTCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                     <  1:43764/143‑1 (MQ=255)
                  tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCTTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACTAGCTGGGCGCACCGTCTCGTacg                                                                                                                    >  1:58264/1‑141 (MQ=255)
                  tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                    >  2:569559/1‑143 (MQ=255)
                  tCCAGCCTGGGGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGACGATATGCCTTACGATGAAGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                    >  1:142177/1‑143 (MQ=255)
                    cAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGaa                                                                                                                  >  1:375673/1‑143 (MQ=255)
                     aGCCTGGTGACAATATGGCAGGTAGTCATTGTATCTAGTGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGGTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAAc                                                                                                                 <  2:394532/143‑1 (MQ=255)
                          ggTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCAAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGCCAGGAGTACCGTCTAGTACAAACATAgg                                                                                                            >  1:49182/1‑143 (MQ=255)
                            tGACGAGATGGCAGGTCGTCACGGTAACAAGCGTGTGCTTACTGAGGTTTTCCCGATGGAAGGTATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGTGTACCGTCTCGTATGAACATCGGTc                                                                                                          <  1:983905/143‑1 (MQ=255)
                            tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                          <  2:711060/143‑1 (MQ=255)
                                 aGATGGCAGGGCGTCACGGTAACAATGGTGTAATTTCTAAGACCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc                                                                                                     <  2:400085/143‑1 (MQ=255)
                                    tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                  <  1:467020/143‑1 (MQ=255)
                                        gagtggtcACGGTAACAAGGGTGCACGTTCTAAGACCAACCCGATCGAAGAGATGCCTTACGATCAAAACGGTACGCCGGTAGACATCGTACTGAACCGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                              <  2:670356/138‑1 (MQ=255)
                                          gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCGGATCCTCGAAAcc                                                                                            >  1:418118/1‑143 (MQ=255)
                                             gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCAc                                                                                         <  2:893062/143‑1 (MQ=255)
                                               cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                       <  2:643780/143‑1 (MQ=255)
                                                acgcgaaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTg                                                                                      <  2:813350/138‑1 (MQ=255)
                                                       cAAGGGTGTAATTCCTAAGCTCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATgg                                                                               <  2:694845/143‑1 (MQ=255)
                                                         aGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGACACCCACCTCGGTCTGGCt                                                                             >  1:704334/1‑143 (MQ=255)
                                                            gtgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCg                                                                          <  1:569559/143‑1 (MQ=255)
                                                             tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAGACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCGGATCCTCGAGACCCACGTGCGCATGGCTGCGa                                                                         >  1:1013529/1‑143 (MQ=255)
                                                               tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                       <  2:85533/143‑1 (MQ=255)
                                                               tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                       <  2:79677/143‑1 (MQ=255)
                                                               tAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                       >  1:79677/1‑143 (MQ=255)
                                                                  tttCTAAGATCAACCCGATCGAAGCTGTGCCTTACGATGAAAACCGTAAGCAGGTGCTCAGGCTGCTGAGCGAGCGGGGCGTACCGGCTCGGGTGGACATCGGGCAGATCCGCGAAGCCCACCTGGGGAGGGGTGCGAAGGGt                                                                    >  1:989528/1‑143 (MQ=255)
                                                                   ttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTa                                                                   <  2:260864/143‑1 (MQ=255)
                                                                          ctCACCCCTATCGACGATATGCCTTACGATGAAAACCGTCCGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                            <  2:607950/142‑1 (MQ=255)
                                                                                    tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                  >  2:745234/1‑143 (MQ=255)
                                                                                      gAAGATATGCCTTACGATGAAAACGGTACACCGGTAGACGACGCACTGCACATGCTGGGCATACCGTCTCGTATGAACAGTTGTGGGATCTTCGAAAACCTGCTGGCGATGGCTGCGAACGGTATCGGCGACACGATGATCGc                                                >  2:375232/1‑143 (MQ=255)
                                                                                          atatGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATg                                            <  2:58264/143‑1 (MQ=255)
                                                                                           tatGCCTTACGATGAAAACGTTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGc                                           <  2:788749/143‑1 (MQ=255)
                                                                                                    ccaTGAACACGGCACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagc                                  <  1:275656/141‑1 (MQ=255)
                                                                                                     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGACCATAGGTCAGATCCTCGAAACCCACCTGGGTCTGGCTGCGGAAGGTCTCCGCCACCAGCTCAACGCCCTGCTAAAAcacga                                 >  1:108831/1‑140 (MQ=255)
                                                                                                     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                 >  2:684843/1‑143 (MQ=255)
                                                                                                     gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                 >  2:311345/1‑143 (MQ=255)
                                                                                                          aaaCGGTACGCCGGTAGACATCGTACTTAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCaag                            <  2:641897/143‑1 (MQ=255)
                                                                                                            aCGGTACGCCGGTAGACATCGTTCTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagctgattttg                          <  1:243643/143‑9 (MQ=255)
                                                                                                               gTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTc                       <  1:500868/143‑1 (MQ=255)
                                                                                                                tACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                      <  2:198264/143‑1 (MQ=255)
                                                                                                                gacggcgtgaGACATCGCACTGAACCAGCGGGGCGTGCCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCGGAAACAGCAGCAAGAAGTcg                      <  1:832043/134‑1 (MQ=255)
                                                                                                                   gCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGAATAAAAATCTGGCAGATCCTCGGATTGTCCCCGCGTCTGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCCGCAAGAAGTCGCGa                   >  2:854135/1‑143 (MQ=255)
                                                                                                                   gCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACACACCTGGGTATGGCTGCGAAAGGTACCGGCGACAAGCTCAACGCCATGCTGAAACAGCCGCACGCAGTCGCGa                   >  1:643806/1‑143 (MQ=255)
                                                                                                                      ggTAGACGTCCGACTGAACCAGCTGTGCGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAAc                <  1:785084/143‑1 (MQ=255)
                                                                                                                             aTCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGACCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGa         >  2:981723/1‑143 (MQ=255)
                                                                                                                                 tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc     <  2:99969/143‑1 (MQ=255)
                                                                                                                                    tGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACGCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGGTGGAACAGCAGACAGAAGTCGCGAAACTGAGCGAATTCATc  >  1:1001399/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATC  >  NC_000913/4184404‑4184678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑