Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,936,308 C→T V3I (GTA→ATA)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,936,3080CT92.7% 117.4 / NA 42V3I (GTA→ATA) zwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (21/17);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGG  >  NC_000913/1936170‑1936443
                                                                                                                                          |                                                                                                                                       
gtcccccGATAATCCGGGCGTCCCGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGcc                                                                                                                                     <  2:901678/138‑1 (MQ=255)
      cGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCCGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAACGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCa                                                                                                                               <  2:486178/143‑1 (MQ=255)
        aTAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCCAAAATGCCCCGGTCACAGGCCTGGGCTGTTCGCGGTAGAGCCagggaatc                                                                                                                             >  1:431222/1‑136 (MQ=255)
         tAATCCGGGTGTCCGGGTTGACCTGACCGGCTTTTTCCAGTTGATACAGGGAACGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGCCACAGCCCTATTCTGTTTGCGTTATCGCCATGTCATtc                                                                                                                            <  1:888499/143‑1 (MQ=255)
           aTCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAGTTTACCCCGCGCAAGGTCGCCTTTCGCGCCGACCATGACCATGGCAGAGGCCTGGGCTTTTTGCGTTTCCGCCATGTCATtctc                                                                                                                          >  2:616579/1‑143 (MQ=255)
                 gtgtCCGGGTGGACCTGACCCGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTCCGACGCGCAGGGTCTCCTTTCTCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAg                                                                                                                    <  2:446381/143‑1 (MQ=255)
                    tCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAACATGACCAGGTCACAGGCCTGGGCTGTTTGCGCCACCCCCATCTCGTGCTCCCTCAGtaa                                                                                                                 >  1:205431/1‑141 (MQ=255)
                    tCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTta                                                                                                                 >  1:167642/1‑143 (MQ=255)
                      cGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaac                                                                                                               <  1:619668/143‑1 (MQ=255)
                      cGGGCTGAGCTGACCGGCTTTTGCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaac                                                                                                               <  1:614527/143‑1 (MQ=255)
                       gggTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTTCGTtagct                                                                                                              >  2:795808/1‑143 (MQ=255)
                                    cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTGTCGCCATGGCATTCTGCTTGAGTTAACTAGCCCGGGACTTa                                                                                                 >  2:475481/1‑143 (MQ=255)
                                       cTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGCCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGc                                                                                              <  2:567970/143‑1 (MQ=255)
                                        tttttCCAGTGGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGcc                                                                                             <  1:285827/143‑1 (MQ=255)
                                         ttttCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCa                                                                                            <  2:996194/143‑1 (MQ=255)
                                            tCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGCACTTAAGCCAggg                                                                                         >  2:412459/1‑143 (MQ=255)
                                                gTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGGTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGtata                                                                                     >  1:819737/1‑143 (MQ=255)
                                                           gAAGGCAGCAATGTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGCTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAAtttt                                                                          <  2:243112/143‑1 (MQ=255)
                                                            aaGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTc                                                                         <  2:167642/143‑1 (MQ=255)
                                                               gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCGGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTa                                                                      >  2:235606/1‑143 (MQ=255)
                                                               gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGTTATACTTGTAATTTTCTTa                                                                      >  2:90707/1‑143 (MQ=255)
                                                               gcagcaATTTACGACGCGCAAGGTCGCCTCTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTa                                                                      >  2:653395/1‑143 (MQ=255)
                                                                     aTTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAACTGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTACGCCCGGGTATCCTTGTAATTTTCTTACGGTGc                                                                >  1:556431/1‑143 (MQ=255)
                                                                          cgacgCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGt                                                           >  2:125939/1‑143 (MQ=255)
                                                                                     gtcttcTTTCGCGCCGAAAACGACCAGGTCACAGGCCGGGGCTGCCTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTAc                                                <  2:85073/138‑1 (MQ=255)
                                                                                       cGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGCACTACTATTACGa                                              >  1:775/1‑143 (MQ=255)
                                                                                          cTTTCGCGCCGAAAATGACGAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACCTATTTTACGGGCt                                           >  1:201692/1‑143 (MQ=255)
                                                                                              cgcgCCGAAAATGACCACGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCg                                       <  1:243779/143‑1 (MQ=255)
                                                                                                cgcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaa                                     >  1:911926/1‑143 (MQ=255)
                                                                                                cgcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaa                                     >  2:677898/1‑143 (MQ=255)
                                                                                                 gcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCCTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGGAATTTTCTTACGGTGCCCCGTACTGTTTTTACgctatctctaaa                                    >  1:467348/1‑132 (MQ=255)
                                                                                                   cGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAAc                                  >  2:896618/1‑143 (MQ=255)
                                                                                                    gAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACt                                 <  2:447645/143‑1 (MQ=255)
                                                                                                               ggTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAACACTGTAACCGCTTa                      >  1:330716/1‑143 (MQ=255)
                                                                                                                 tgacaGGCCTGGGCTGTTTGCGTTATCTCCATGTCATTCTCCTTCTGTTTACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATc                    <  1:875306/141‑1 (MQ=255)
                                                                                                                 tCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATc                    >  1:243710/1‑143 (MQ=255)
                                                                                                                   acaGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTCTACGAGCTTGCGAAAACTCTAAACGCTTATCCa                  >  1:145022/1‑143 (MQ=255)
                                                                                                                    caGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTCATCCAc                 <  2:638644/143‑1 (MQ=255)
                                                                                                                      ggCCTGGGCTGTTTGCGTTATCGCCATGTCATGCTCCTTAAGTTAACTAGCCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTCTTACGAGCTTTCGAAAACTGTAAACGCTTATCCAccc               <  1:993412/143‑1 (MQ=255)
                                                                                                                                gtgtGCGTTACCGCCAGGTCATCCGCCATAAGAGAACTAACCCGGTCCTTAAGCCAGGGTATACGTGCAATTTTCTCACGGTCCACCGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTAc     <  2:307472/140‑1 (MQ=255)
                                                                                                                                   tGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAGACTGTAAACGCTTATCCACCCGTGTGATTACggg  >  2:175893/1‑143 (MQ=255)
                                                                                                                                   tGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACggg  >  2:175941/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGG  >  NC_000913/1936170‑1936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: