Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,482 C→A 21.4% Q8K (CAA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4820CA21.4% 62.8 / 4.1 41Q8K (CAA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (12/20);  new base A (4/5);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

ATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC  >  NC_000913/3206246‑3206714
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                         
aTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                                                                                                                                                         <  1:285995/239‑1 (MQ=255)
 ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAATTTTTCACAAAAAAACAAAAAATGGAGTTGTCCTTGTGTGGGAAAGTAATAAGAAAc                                                                                                                                                                                                                                        >  2:250348/1‑239 (MQ=255)
  tCAGCATCGTTAATTATCCGCAGTTTTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                                                                                                                                                       <  2:341723/239‑1 (MQ=255)
      cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTATACCAAAAAAAAAAATCGAGTTGGCCTGATTAGCGACAGGAATAAACaaacagca                                                                                                                                                                                                                                   >  2:25988/1‑233 (MQ=255)
       aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                    <  1:195128/237‑1 (MQ=255)
           ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGACAAAAAAAAAAAATTGAGTTTCCATGGTGAGCGAAAGGAATAAGAAAAAGGCAATGaa                                                                                                                                                                                                                              >  1:103531/1‑239 (MQ=255)
                                 aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAACAAAAAAAGTAGTTGCCATGATGAGCGGAAGTAATAAACAACAAGCAGTGAATAAGATTACAGAGATTTTAGCt                                                                                                                                                                                                        >  1:13288/1‑239 (MQ=255)
                                                  aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTGAAAAAATACAAAACAGGAGATGTCATGTAGAGGGGAAATAAAAAAAAAACAGGAAGAGAAAAGATTAACAAGATTGTTGCTACATTTAACACCATggt                                                                                                                                                                                       >  1:389349/1‑237 (MQ=255)
                                                    aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCCACCAAAAAAAAAAACTGTAGATGGCGTTTAGAGGGAAAGTAATAAACAACAAGAACTGAATAAATTGATAGAGAGTGTTAGCAACTTTACCGCCCTGAatt                                                                                                                                                                                     >  2:54487/1‑239 (MQ=255)
                                                      ctttGCCTTTCCCTGCTCTGGCGAAATATTCATTCTTATTTTCGTGTTAATTCACAAACTGTTAATTAAATATTCAGGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGGGGTGAATTTTTCCAAAAAAATACAAAAACTGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                                                                                                                                                                   <  2:13288/237‑1 (MQ=255)
                                                            cATTCCCCGCTCGGGCGAAATATTCATTCTTATTTTCGTGTTGATTCACGAACTGTTAATTAAATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGGGGTAGTTGGTGGGGGTGAATTTTTCAAGACAAATAAAAAAACTGGGTTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTGGTTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                            <  2:389349/239‑1 (MQ=255)
                                                             aTTCCCTGCTCTGGCTAAATTTTAATTCTTTTTTCCGCGTTATTTCACGAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCACATTTTTATGGGTAGTTTGTGGGGGTAAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                           <  2:210357/239‑1 (MQ=255)
                                                                   tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAAATTTTCAGACAAATAAAAAAAATTGAATTGCCATGGTGAGGGGAAGTAATAAGCAAAACGCAGTGAATAAATTTGCAGAGATTGTTGCTAACATTACCGGCCTGATTTTTATCCCGatagctg                                                                                                                                                                      >  1:435241/1‑233 (MQ=255)
                                                                         ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAGATTTTTTAGACAAAAACAAAAACAAGAGTTGCGATGGTGAGCGCAAGTAATAAACAAAAGAGAGGGAATAAATTGACAGAGAATTTTGCTCACTTTACCGCCACGATTTTTTACCGCAAGCCtgtttatg                                                                                                                                                               >  1:150395/1‑233 (MQ=255)
                                                                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTAGAAAAAAAAAAAAAATGGAGTTTGCATGAGTAGGGGAAGGTATAAACAAAAAGCAAGGAAAAAAGTTACAGAGATTGTCGCTAAATTTACACCCCTGATTTTCAACCGAAAGCCtggttgaat                                                                                                                                                              >  2:57195/1‑232 (MQ=255)
                                                                            gcAATATTCATTTTTATTTTCGCTTTGATTAACAAACTGTTAATTATATATTCTGGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                             <  2:435241/238‑1 (MQ=255)
                                                                                tatTCATTCTTATTTTGGCGTTAATTCACAAACTGTTAATTACATATTGAGGGTGGAAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTACCAGAAAAATACAAAAACTGGAGTTGCCAGGATGAGCGAAATTAATAAGCAACAGGCAGAGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                        <  1:25988/239‑1 (MQ=255)
                                                                                tatTCATTCTTATTTACGCGTTGATACACGAACTTTTAATTACATTTTCCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTACCTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                        <  2:266158/239‑1 (MQ=255)
                                                                                         ttgtataagatacatttcccaaccTGTTAATTACATTGTCATGGGTGAAAAAAATATAAAACCACAATTTTAGGGGTAGTTGGTTGGGGTGAATTTTTCCAGACAAAAACAAAAACTGAAGTTGCCATGATTACCGAAAGAAATAAGAACCAGGCAGGGAAAAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                               <  1:183503/216‑1 (MQ=255)
                                                                                           aaggaaatgtatataagagacaggTTAATTACATATACACGGTAGAAAAAAATATAAAACCACATTTTGAGTGGTATTTTGTGGCGGTAAATTTTTCAAGAAAAATACAAAAACTGAAGTGGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTGGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                              <  1:389608/215‑1 (MQ=255)
                                                                                            gacagatttgtaaaagaaacattttAATTACATATTCACTGTGACAAAAAATATAAAACCAAATTTTTAGTGGTTTTTTTGGGGGGTGAATTTTCCCAAACAAATAAAAAAACTGGAGTTGCCATAATGAGCGAAAGTAATAAACAACAGACAGTAATAAAGTTAAAAGAGATTGTGGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                              <  2:99391/214‑1 (MQ=255)
                                                                                             tttCGCTTTAATTCGAGAACTTTTAATTACATATTCACGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTATTTTGTGGCGGTGAATTTTTCAAAAAAAATAAAAAAACTGGATTTGCCATGATGAGCAAAAGTAATAACCAACGGGCAGTAAATAATTTGACAGAAATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg                                                                                                                                           <  1:12985/239‑1 (MQ=255)
                                                                                                                   tgaaTTACATATTCACGGTGGAAAAAATAATAAACACCCATTTTAATTGGTATTTTGGTGGGGTTAATTTTCCAAAACAAATAAAAAAACTGGAGTTGCCATGAGGAGCTAATGTATTAAGAAACAGGCAGTAAAAAATTTGACAGAGATTGTCGTGAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                              <  1:363901/212‑1 (MQ=255)
                                                                                                                            tatTCAGGTGGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                            <  2:103531/239‑1 (MQ=255)
                                                                                                                            tatTCACGGTGGCAAAAAAAAAAAACCCACATTTTGAGTGGTAGTTTGTGGCGGTGAATTTTCCCAACAAAAAACAAAAACTGGGGTTGCCATGATAAGCAAAATTAATAAGCAACAGACAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                            <  1:287084/239‑1 (MQ=255)
                                                                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAAGAAAAACAAAAAAACGAGGTTTCATGTTGAGCGAAAGTAAAAAACAACACGAAGTGAATAAGATGTCAGAGACTTTCGCTAACATTATCACCAAGATTTCTATCCCAAAGTCTGATAACGTTGTGGTGAAACTAAAACAGATaaaag                                                                                                                                    >  1:99391/1‑212 (MQ=255)
                                                                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGTTTGCAAGGTGAGGGAAAATAATAAACAAAAAGGAAGGAATAAAATGACAGAGAATGTCGCTTAATTTAACCACACGATTTTTACCCGAAAGCCTGAATACGTGGTGGGTAAAACAAAACAAATAAAAGAAGGCGaaaaacacctgctggggga                                                                                                           >  2:92411/1‑223 (MQ=255)
                                                                                                                                    ggagaaaaaaaTAAAAAACCATATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAAAAAAATAAAAAAACTGGAGTTGACATGATGAGCAAAAGAAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                     <  2:219371/233‑1 (MQ=255)
                                                                                                                                     tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAACAAAAAAATGAGGTGGCATGATGAGGGGAAGGAATAAAAAAAAAGCAAAGAAAAAATTGAAAGAGAATGTTGCTAAATTTTACGCCATGATTTTTACCCCAAAGCCTGATGACGTGGGGGATAAACTAAAAAAGCTAAAAGATGCCGGAACGACGTCGATTGGGGAAATTATCt                                                                                                   >  1:170046/1‑239 (MQ=255)
                                                                                                                                             aaTAAAAACCAACATTTTTAGTGGTATTTTGGGGGGGTAAATTTTCCCAAAAAAATACAAAAACGGGAGTTGCCATGAGGAGCGAAAGAAAAAAACAACAGCCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTAGCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                           <  1:92411/239‑1 (MQ=255)
                                                                                                                                               taaaaaaCCAAATTTTTAGTGGTAGTTTGGGGGGGTGAATTTTTCAAGAAAAATAAAAAAACGGGAGTTGCCAGGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                            <  2:187751/217‑1 (MQ=255)
                                                                                                                                                         caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAACTTGAGGTGCCATGATGAGCGGAAGGAATAAACAACAAGCAGTGAAAAAATTGACAGAGATTTTCGCTAACTTTACCCCCATTATTTTTACCCCAATGCCTTGTGACGTGGGGGGTAAAATAAAACAACTAAAAGATGCCGAAACGTCGTCGATGGGGGAAATTATTTAcccgtttcttatacaaa                                                                                 >  1:187751/1‑222 (MQ=255)
                                                                                                                                                                  gggTAGTTGGGGGCGGTGAATTTTTCAAGAAAAAAAGAAAAACTGGAGTGGCCATAATGAGAAAAAGTAATAAGAAACGGGCAGTGAATAAGTTGAAAGGGATTGTGACTAATTGTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                       <  1:250348/237‑1 (MQ=255)
                                                                                                                                                                      ggttggggggggaaaaTTTTTACAGAAAAATAAAAAAACTGGAGTGGCAAGGATGAGCGAAAGTAATAAGCAACGGGCAGTGAATAATTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                  <  2:170046/225‑1 (MQ=255)
                                                                                                                                                                                                aaaaaaaaaaaaaTTGAATTTGCAAGAATAAGCGAAAGTAACAAGAAACGGGAGGAAAATAAGTTGCAAGAAATGGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                         <  1:57195/231‑1 (MQ=255)
                                                                                                                                                                                                 cAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                        >  1:507347/1‑239 (MQ=255)
                                                                                                                                                                                                        ataagagaaatttGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAAAAAGTTGACAGAGTTTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                 <  2:411135/228‑1 (MQ=255)
                                                                                                                                                                                                          aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                               >  2:307976/1‑239 (MQ=255)
                                                                                                                                                                                                           aaCTGGGGTTGCAAGGAGGAGGAAAAGTAAAAAGCAAGGGGAGGGGAATAGATTGAAGGAGATTGCGGCAAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:150395/239‑1 (MQ=255)
                                                                                                                                                                                                                              aGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGAGAGATTAt                       >  1:411135/1‑227 (MQ=255)
                                                                                                                                                                                                                                        aataaCAAACAGGAAGTGAATAGTTTGACAGAGTTGTTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc  <  1:304520/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                         
ATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC  >  NC_000913/3206246‑3206714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: