| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,760,383 | A→C | 29.8% | V289G (GTG→GGG) | selA ← | selenocysteine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,760,383 | 0 | A | C | 29.8% | 33.2 / 2.2 | 29 | V289G (GTG→GGG) | selA | selenocysteine synthase |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base C (2/7); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCT > NC_000913/3760146‑3760611 | gCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCAATCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGCGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTATTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACCTCTCGCCGGGTAAAATCa > 1:493258/1‑238 (MQ=255) ccTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCAc < 2:57639/238‑1 (MQ=255) ggATTTTAATTACTTCTGGCGTGTGGTTAAGCAGGGGAAGGGTCGGTAATTTTTCACTCAGATCTTCAGGGTGTAAATAAAGAGCCAGCGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCATGCTGCAGCGGGTGGTTTGGCAGGCGGGCGATCATTTCTTTTTTACCAACAATAATTCCTGCCGGCGGCCCGCC‑TAACAGCTTGTCGCCGGAGAAACTCACCAGa < 2:342541/239‑1 (MQ=255) ggATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTTCCAACAATAATTCCTTCCTGCTGCCCGCC‑TAACCACTTGTCGCCGGAGAAACTCCCCaa > 2:499748/1‑237 (MQ=255) aaTGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCCGCCGGCCGCC‑CAACAAATTGTGGCCGGAGAAACCCACCAGACTGAcg > 2:390356/1‑238 (MQ=255) tgcgGGTAAGCAGGTCAAGGGCCGGTATTTTTTCACTCAGAGCTACAGGGTGTAAATAAAGACGCAACGGGGCTCCCAGCGCCGCGGGGGTC‑ATTTTACCCGCGTGTAATGCACGCTCCAGGGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAAAAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGCCGCCCGCCGCAATCaa < 2:269345/238‑1 (MQ=255) tgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTTACCAACAATAATTCCTTCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGAATGACGCCCGCCGCAATCaa > 2:88736/1‑238 (MQ=255) cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TACCAACTTGTCCCCGGAGAAACTCACCCGACTGCCCCCCGCCCCAATCAACt > 2:93892/1‑238 (MQ=255) gTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATTTCTTTTTTATCAACAATTAGTCCTCCCTGCGGCCCCCC‑TAACAACTTGTCGCCGGGGAAACTCACCAGACTGACGCCCGCCGGCATCAACTCCTCCCGCAGTGACCCtttacg > 2:152097/1‑235 (MQ=255) tccgcaggagCTGCGGGGTAAAAAGAAAGACGCAACGTGGCTTACGGCGCCGGGGGGGTT‑ATTTTATCCGCGTGTAAGCCACGCTTCAGGGGGTGGCTGTGCGGGGGGGCGATCATCTCTTTTTTACAAAAAATAATTCCTGCCTGCGCCCCGCC‑AAACACCTTGTCGCGGGAAAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa < 2:365581/232‑1 (MQ=255) tcccgcagtcctgcggggTGTAAAAAGAGACGCAGCGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTCCAGGGGGGGGCTTGGCAGGCGGGCGATCATCTCTTTTTTCCCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTGGTCGCCGGAAAAACTCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa < 2:500506/224‑1 (MQ=255) cTTCGGGGTAAAAAAAAAGCCGCAACGTGGCTCCCAGCGCCGCGAGGGTC‑TTTTTATCCGGGCGAAATGCCCGCTTCGGGGGGTGGCTTGGCGGGGGGGCGATCATCTCTTTTTTACCAACAATAATCCCTGCCGGCGCCCCGCC‑TAACAACTTGTCGCGGGAGAACCCCCCCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAg < 2:349829/238‑1 (MQ=255) aacggcGCAGCGTGGCTCCCGGCGCCG‑GGGGGTCTATTTTATCGGCGTTTAGTGCCCGCTTCAGGGGGTGTTTTTGCGGGCGGGCGTTCATCTTTTTTTTCCCAAAAAAAATCCCTCCCTGCGGCCCGCC‑AAACATTTTGTCGCCGGAAAACCCCCCCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa < 2:304450/234‑1 (MQ=255) gcacgggggCCTCCAGCGCCGAGAGGTGC‑ATTTGACCCCCGTGTAACGCACGCTCCGGGGGGTGGCTTGGCAGGGGGGCACCCATCTTTTTTTTCCCAACAATAATCCCCCCCGGCGGCCCGCC‑TAAAACCTTGCCCCGGGAAAAACCCCCCAGACGGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCt < 2:493258/232‑1 (MQ=255) cGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCCACAATAATTCCTTCCTGCGGCCCGCC‑CAACAAATTTTCGCCCGAGAAAATCCCCCGACTGACACCCGCCGCCATCAACTCCCGCTGCATTTGCTCGTTCGGACAAACGCAACGGCCAAAATCGACCAGccggccccccgccaaat > 2:436655/1‑223 (MQ=255) cgcgTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCCCCGCCTAAAACAATCACTACGGGAACCTCCCGCTCTTTGCCGAg > 2:381849/1‑238 (MQ=255) ggCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTTTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCCCTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCCAGCGCCCACCGTTCCGCCTCATCTAt > 2:230588/1‑238 (MQ=255) gggcgATCATCTCTTTTTCACAAAAAATAATCCCGGCCTGCGGCCCGCC‑TAAAAACTTGTCGCCGGAAAACCTCACCAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa < 2:449297/238‑1 (MQ=255) atcTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAAt > 1:166308/1‑160 (MQ=255) tttctTTTTTCCCAAAAAAACTCCCTGCCGGCGCCCCGCC‑AAACAACTTGCCGCCGGAGAACCCCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATg < 2:165259/237‑1 (MQ=255) tctTTTTTACAAACAAAAATCCCGGCCGGCGCCCCGCC‑TAAAAATTTGTCGCGGAAGAAACCCCCCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCt < 1:285478/239‑1 (MQ=255) ctTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGact > 1:165259/1‑233 (MQ=255) tttACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGGCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGt > 1:456200/1‑239 (MQ=255) caCCAACAAAAATCCCCGCCTGCGCCCCCCT‑TACCAACTGGCCGCGGGAGAACCTCACCAGACTGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa < 1:381849/238‑1 (MQ=255) acttcccgccgcccgCCCCCC‑CAACACGTTGTCGCCGGAAAACCACACCAGTCGGCCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa < 1:244424/225‑1 (MQ=255) aatCCCTGCCTG‑GGCCCACCTTAACAATTGGTCGCCGGAGAAACTCCCGACACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa < 1:34006/239‑1 (MQ=255) aatCCCTGCCGGCGGCCCGCC‑AAACACCTTGCCGCCGAAAAACCGCCCCAGACTGCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa < 1:502259/239‑1 (MQ=255) aatACCTGCCGGCGCCCCCCC‑TAACACCTGGCCCCAGGGAAAACGCGCGAGCCTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa < 2:8078/239‑1 (MQ=255) gcctgcGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGccacca > 1:45874/1‑175 (MQ=255) gaaaaaCTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCt < 1:499748/235‑1 (MQ=255) | GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTC‑ATTTTATCCGCGCGTAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTTACCAACAATAATTCCTGCCTGCGGCCCGCC‑TAACAACTTGTCGCCGGAGAAACTCACCAGACTGACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCT > NC_000913/3760146‑3760611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |