Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,782,916 G→A 22.8% L249L (CTG→TTG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9160GA22.8% 25.4 / 2.2 26L249L (CTG→TTG) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base G (4/16);  new base A (4/2);  total (8/18)
Fisher's exact test for biased strand distribution p-value = 5.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCG  >  NC_000913/3782685‑3783142
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   
tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAGATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGTTTGCGGGACAGGTAAGGACCAGATCCCCa                                                                                                                                                                                                                              >  1:328121/1‑239 (MQ=255)
    tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTTGTTGTCGGGAAAGGTAAACACCAGAACCCCAAGc                                                                                                                                                                                                                           >  1:492692/1‑238 (MQ=255)
    tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACCGGTTGGCGGTACAGGTAAGCACCAACTCGCCCAGc                                                                                                                                                                                                                           >  1:26543/1‑238 (MQ=255)
       tgcCTCGCGCGTGTTTTTTCCGAAATACAATACTGGAGAAATTCCCTCGGTTATGGGCAGTTCACCGACGAGGCGATGCGCCAGTCCGCGGACTTCTTGCGTATTGCGGTAGCCTTCCACCCCCGGAACAATCTCCTCCTCCGCGCTTTGTACACCCATTCCCTGACCGAGCTACATCCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGAACGCCACGCCCCg                                                                                                                                                                                                                       <  2:451974/239‑1 (MQ=255)
           gcggcggggTTTTTCCCGCAATTTAAAGCTTGTAAAATTTCCTCGGTTTTTGGCATTTCACCGCCAAAGCGATGCGCCGGTTCGCGGTCTTCTTTCGTATTGGGGTAGCCTCCCCCCACCTGCCCAATCTTCCCCTGCGCGCTTTGTACACCCAGGCCCTGACCGAGCCTCATGCCAAAACGGCGGTTACGGGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                   <  2:336391/231‑1 (MQ=255)
                          ccGCATTATAATACTTGATAAATTTCCCCGGTTATTGGCATTTCACCGCCGAAGCGATGCGCCATTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTCCTCCTGCGCGCTTTGTACATCCATGCCCTGACGGGGCATCATGCCAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAGGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGc                                                                                                                                                                                                   <  2:76340/239‑1 (MQ=255)
                                        ttGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGGACAGGTAAGCACCAGATCGCCAAACCCCGCCATGCCCCTAAAAGTGGCAGGGTCGGCAccc                                                                                                                                                                                      >  1:323323/1‑238 (MQ=255)
                                          gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCACCGGTTTTCGGTGACAGTAAACACCAAAACGCCAAACCCCCCCCTGCCCACAAAGGTGGGAGGGGGGGCAccccc                                                                                                                                                                                    >  2:503202/1‑236 (MQ=255)
                                            tAAATTTCCTCGGTTATTGCCATTTCAACGCCGAAGCGATGCGCCATTTCGCGGACTTCTTTCGTTGTGCGGTACCCTCCCCCCCCCGGACCATTCTTCTCCTGCGCGTTTTGTCCATCCATGCCCTGACCGAGCATCAGGCCAAACCGGCGGTTACGCGACTGTTTGTCGGTACAGGTAAGCCCCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAgcg                                                                                                                                                                                  <  2:20682/238‑1 (MQ=255)
                                                      cGGTTGTTGGCATTTCACCGCCGAGGCGCGCCGCCATTCCGCGGACTCCTTTCGTATTGCGGTACCCTCCCGCCACCTGCCCAATCTTCCCCTCCGCGCTTTGTACACCAAGGCCCTGACCGACCATCATCCCAAACCGGCGGTTACGCGCGTGGTTGTCGGTACAGGTAAGCACCAGATCGCAAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGa                                                                                                                                                                       <  2:183205/239‑1 (MQ=255)
                                                          tATTGGCATGACAGCGCGGACGCGAGGCGCCGGTTCGCGGACTTCTTTCGTATTGGGGTAGCCTTCCACCCCCGGACCACTCTTCTCCTGCGCGCTTTGTACATCCATGCCCGGACCGGGCATCAGGCCAAAACGGCGGTTACGCGATTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                   <  2:227356/239‑1 (MQ=255)
                                                                           cGAAGCGGTGCGCCCGTTCGCGGACTTCTTTCGTAGTGCGTGAGCCTTCCACCACTTGACCAATCTTCCCCTGCGCGCTTTGTCCATCCATGCCCTGACGGGGCATCATGCCAAAACGGCGGTTACGCGACTGGTGGTCGGTACAGGTAAGCACCAGAGCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCa                                                                                                                                                  <  2:71146/239‑1 (MQ=255)
                                                                                      gcCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGGGCATCAGGCCAAACCGGCGGTTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAg                                                                                                                                       <  2:432990/239‑1 (MQ=255)
                                                                                      gcCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCGGACCAATCTCCTCCTGCGCGCTTTGTACGTCCATGCCCTGACCGAGCATCGCGCCAAAACGGCGGTTCCGCGACTGGTTGGCGGTCCAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAg                                                                                                                                       <  2:757/239‑1 (MQ=255)
                                                                                                                    tggCCTTCCCCCCCCTGACCAATTTTCTCCGGCGCGTTTTGTAATCCCATGCCCTGACCGAGCATCATGCAAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                          <  2:492692/236‑1 (MQ=255)
                                                                                                                                    gcccAATCTTCTCCGGCGCGTTTTGTACATCCAGGCCCTGACCGAGCCTCACGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATcccc                                                                                         <  2:166419/237‑1 (MQ=255)
                                                                                                                                                      cgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGGTGTCGGGACAGGGAAGCACCAAAACCCCAAGCCCCCCCCTGCCCCTAAAGGGGGCAGGGGCGGCACCCCGCGCCGCCCCCAGACGCCACCATTTAGCCCACCCACCGGGGAACAGCGCCCTACGCGCAATCCCCCCCAAACCCACACCCCCGGACATCCCCCCCCCACTCGCCATaaac                                                                       >  2:47707/1‑237 (MQ=255)
                                                                                                                                                                  ccACGCCCTGACCGAGCATCAGGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcgcg                                                           <  1:6649/239‑1 (MQ=255)
                                                                                                                                                                     tGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAACCGACTGGGTGTCGGGAAAGGGAAAGACCAAAACGCCAAGCCCCCCCCTGCCCCTAAAAGGGGGAAGGGCGGGCACCAGAGCCCCCCCCACAAGCGACACTTCAGCCAACCCACCGGGGGTCCGCGCCCCCCCCGCCTTCCCCACAAAACCCACACCCTCGGACCTCCCCCCCCCACCGGCACACACGTGTTTCCCCGcccc                                                         >  2:233421/1‑238 (MQ=255)
                                                                                                                                                                        catgcccGAGCATCAGGCAAAAACGGTGGTGACGCGACTGGTTGCCGGTACGGGAAAGCACCAGAACGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCa                                                     <  2:304193/234‑1 (MQ=255)
                                                                                                                                                                                          aaaaaCGGCGGTCACGCGACTGGTTGCCGGTACAGGTAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGaa                                    <  1:144246/237‑1 (MQ=255)
                                                                                                                                                                                                ggcagTTACGCGATGGGTTGTCGGTACTGGAAAGCCCCAAACCGCCCAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCTCCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa                             <  1:350953/239‑1 (MQ=255)
                                                                                                                                                                                                                   gTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCACCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaa            >  1:61180/1‑237 (MQ=255)
                                                                                                                                                                                                                          ccggttgaGCACCAAACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGGGATCAGCCCCGTACGCGCATTCGCACCAAATCCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTg     <  1:158293/231‑1 (MQ=255)
                                                                                                                                                                                                                             ggaaaGCCCCGGACCGCAAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAGACGCGGAAACTTTTGCCg  <  2:327211/235‑1 (MQ=255)
                                                                                                                                                                                                                              gTAACCACCAGACCGCAAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg  <  2:323323/237‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                   
TCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCG  >  NC_000913/3782685‑3783142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: