Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,478,306	A→T	22.9%	L27F (TTA→TTT)	ynbB →	putative CDP‑diglyceride synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,306	0	A	T	22.9%	69.0 / 2.2	32	L27F (TTA→TTT)	ynbB	putative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base A (2/22);  new base T (5/2);  total (8/24)
Fisher's exact test for biased strand distribution p-value = 2.27e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

GCGTTAATATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGC  >  NC_000913/1478074‑1478538                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             gcgTTAATATTTGGTCTGTGTGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGga‑g                                                                                                                                                                                                                                       <  2:32390/239‑1 (MQ=255) gcgTTAATATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGga‑g                                                                                                                                                                                                                                       <  2:377389/239‑1 (MQ=255)    ttAATATTTGGTCTGTGGGGTCTGTCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGAATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATGTAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGACACGCTGATTAACCGTTTTCTGCTATGGCGGATGTCGgg‑gaga                                                                                                                                                                                                                                    >  1:332419/1‑239 (MQ=255)        tatTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCGCAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAg                                                                                                                                                                                                                                <  1:276173/239‑1 (MQ=255)            tGGTCTGTGGGGTCTGTCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGACGGTTTCCGGA‑GAGGAAAggggg                                                                                                                                                                                                                            >  2:512077/1‑239 (MQ=255)            tGGGCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAggggg                                                                                                                                                                                                                            <  2:241863/239‑1 (MQ=255)                       gTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACa                                                                                                                                                                                                                 <  1:93943/238‑1 (MQ=255)                       gTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACa                                                                                                                                                                                                                 <  2:280407/238‑1 (MQ=255)                         cTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTa                                                                                                                                                                                                              <  1:9972/239‑1 (MQ=255)                                                                       tATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGG‑GAGAAAAGGGGGGGAGGTTTTCTTTTCGT‑TTTGTACCTGGTGGGGGAACGT‑CATTTTGttttttctggttg                                                                                                                                                               >  1:289155/1‑231 (MQ=255)                                                                                    ttCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACg                                                                                                                                                 <  1:253976/239‑1 (MQ=255)                                                                                      cAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGAGGGGAAAAGGGGGGGGGG‑TTAATTTCCGAATTGTTACTTGG‑GGGGGGTCGTCCT‑TTTGttttttattgtggttttatggtcaccag                                                                                                                                                >  2:284846/1‑216 (MQ=255)                                                                                              cTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGA‑GGGGAAAAGGGGTGAGG‑GTACATTACGTATTTCTACCTGGTGGGGGATCGT‑CC‑TTTTTTTTTTTATTGGGATTTTTGGTCCACGCTTGGtgacg                                                                                                                                       >  2:20444/1‑239 (MQ=255)                                                                                                tctcTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacgtt                                                                                                                                     <  2:434278/239‑1 (MQ=255)                                                                                                 ctctGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAggggg                                                                                                                                                                                                                            <  1:343048/154‑1 (MQ=255)                                                                                                              ttAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcg                                                                                                                        <  2:292712/238‑1 (MQ=255)                                                                                                                        tCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcg                                                                                                                        <  1:30440/228‑1 (MQ=255)                                                                                                                                   ttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTGTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATGCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa                                                                                                  <  2:56452/239‑1 (MQ=255)                                                                                                                                    tcttATGAGCGCTGAAATATAATGCTGAACAAATCTCTGGCAACTCGTTTCGTGTTGTTAAGTTGAGCAACGCGGATAAACCGTTTTCTTCTATGGCGTTTTCCGGA‑GAGAAATGGGGTTGAGG‑TTACATTACGTTTTCGTACCTGGTGGCGCATCGT‑CA‑TTTGTTTTGCAATGGTGATTTCAGGTCCACGCTGGAAGACTTTGACGTTTTTTGCGCTGATAAGTTTTCTGTCATTGaa                                                                                                 <  1:270722/239‑1 (MQ=255)                                                                                                                                              gcTGAAATATAATGCTGGAAAAATCGCTGGCAACTCGTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt                                                                                       <  1:222355/239‑1 (MQ=255)                                                                                                                                                     tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGt                                                                                       <  1:380250/232‑1 (MQ=255)                                                                                                                                                              ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACGGA‑GAGGAAAGGGGGGGAGG‑GTTCATTACGTATTTGTACCTGGGGGGGGATCGT‑CC‑TTTGTTTTTTTATTGGGATTTTAGGTGCACGCTGGGTGACGGTGGCGGTTTTTTTGCTGTTAAGTTTTTTTGCATTGTAAGAAAATTGTAAGCTTTTATCTGTaa                                                                       >  1:292521/1‑238 (MQ=255)                                                                                                                                                               gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GGGAAAAGGGGGGGAGG‑GTTCATTACGTATTCGTATCCGGGGGGGGATCGT‑CC‑TTTTTTTTTTCATTGGGAGTTCCTGTCCCCGCTGGGTGCCGTGTTCGTTTTTTTTGCTGCTAAGTTTTTTTGCATTGGCAGAAAATTTTTCGCTtttaatctttct                                                                      >  2:380250/1‑231 (MQ=255)                                                                                                                                                                             aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg                                                         <  1:59143/238‑1 (MQ=255)                                                                                                                                                                             aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg                                                         <  2:339430/238‑1 (MQ=255)                                                                                                                                                                             aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTgg                                                         <  1:20444/238‑1 (MQ=255)                                                                                                                                                                                  ttttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTatat                                                   <  1:94101/239‑1 (MQ=255)                                                                                                                                                                                         gttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTGCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTa                                            <  1:387866/239‑1 (MQ=255)                                                                                                                                                                                           tgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTAtt                                          <  2:249963/239‑1 (MQ=255)                                                                                                                                                                                                       cAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGC‑GAGAAAAAGGGGGGGGG‑GTATCTTTCGTATTTGTCCCTTGTGGGGCATCGT‑CA‑TTTTTTTTTTTATGGTGGTTTCAGTGCCTCGCCGGGTGACGGTTGTGTTTTTTGTGCTGATCAGTTTTTTGGCATTTAAAGAATATTTTTAGCTTTTCTCTGTTCATTTTTTGCGTTGGGTTTTTTTTGGTCTTCCTCTTtattat                              >  2:251364/1‑239 (MQ=255)                                                                                                                                                                                                              gATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTa                       <  2:289155/239‑1 (MQ=255)                                                                                                                                                                                                                                    cGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGc  <  2:292521/238‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                             GCGTTAATATTTGGTCTGTGGGGTCTGGCCGTTGCCATTTATCCACAGTGGATGCAGTGGAATAATCTTTTATGGAGCATTGCTTCAATTCTGCTTCTCTGGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGA‑GAGAAAAGGGGGTGAGG‑TTACATTACGTATTCGTACCTGGTGGGGCATCGT‑CA‑TTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTTCCGCGTTGGTTATATTGGGGTATTCCTCTTAACTATTTGCTTATCGGTTTTAATTGCTTTGAGC  >  NC_000913/1478074‑1478538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑