BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	353,673	A→G	12.3%	E323G (GAG→GGG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,673	0	A	G	12.3%	263.8 / 2.3	114	E323G (GAG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (47/50); new base G (15/2); total (62/52)
Fisher's exact test for biased strand distribution p-value = 2.88e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

GACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGG  >  NC_000913/353434‑353907

gACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTC‑TTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCga                                                                                                                                                                                                                                                <  1:900224/239‑1 (MQ=255)

  ccGGCAACCCTAAAGGCGTGCAGGGTGACGTCGGCGGATATGCGGTGGCGCTGGCGTCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCga                                                                                                                                                                                                                                                <  2:1341461/238‑1 (MQ=255)

  ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCgag                                                                                                                                                                                                                                               <  2:141750/239‑1 (MQ=255)

  ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCgag                                                                                                                                                                                                                                               <  2:755558/239‑1 (MQ=255)

              aaGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGt                                                                                                                                                                                                                                   <  2:181601/238‑1 (MQ=255)

               aGGCGTGCAGCGGGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACGCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTa                                                                                                                                                                                                                                 <  2:140929/239‑1 (MQ=255)

                  cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCCGCGGGTTCTTTTGCGCATCGGATATCGGGTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGc                                                                                                                                                                                                                               <  1:718800/238‑1 (MQ=255)

                  cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGc                                                                                                                                                                                                                               <  1:469786/238‑1 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGGTTACGGAGGGTTGCCGGCCTGGGCGGGCTGCGG‑CGG‑GGGGGGGGCAATCGTCGGGAAATA‑TCAGGTTAGcccg                                                                                                                                                                                                                            >  1:1150902/1‑237 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCgg                                                                                                                                                                                                                            >  1:887086/1‑239 (MQ=255)

                    tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGGGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTTGGGCATTCGTATATCGTTTACGCGCCCCTGCCGGCGGGGGGGGCGCCCATCGGTTTTGAAGGCTTTCCGCACTCGCCCGGCTGCCG‑CCTGGGGGTGGAC‑ATCGTGCGGAACTA‑TTAGGGTAGcccg                                                                                                                                                                                                                            >  2:963796/1‑237 (MQ=255)

                       aGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGAt                                                                                                                                                                                                                          <  2:851556/238‑1 (MQ=255)

                          gTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGtt                                                                                                                                                                                                                       <  1:1095284/238‑1 (MQ=255)

                           tGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTATCCTTTACGAAGGGTTGCCGAACGGGCCGGGCTGCGG‑CGG‑GGGGGGGACACTCGGCGGGAAATA‑TTGGGTTGGCCGGGTGGtct                                                                                                                                                                                                                     >  2:241300/1‑239 (MQ=255)

                                   ggggATATGCGGGGGGGCTGGCGCCCTCGAGGGACACCATTTTTGGCGGCAAGGCGGGCGGCGTGTTCTTTGGCGCATCGGATATGGGCGGGGTGGTGGGGCATTCGTATATCGTGTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑cc                                                                                                                                                                                                            <  2:132768/236‑1 (MQ=255)

                                      gttaggCGGTGGCGCTGGCGCCTCCGATGGACACCTTTTTTGGCGGCAAAGCGGGAAGCGTGTTTTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑Ac                                                                                                                                                                                                        <  2:476594/233‑1 (MQ=255)

                                                ggCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGGCTGCGG‑CGT‑GTGGTGGACAATCGGCGGGGAATA‑TCAGGTTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCATTcgcg                                                                                                                                                                                             >  1:1369334/1‑239 (MQ=255)

                                                 gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGAATATCGTTTTAGAAGGAATGCCGGACCGGGCGGACCGAGG‑GGG‑GGTGTGGGAAAACGTGGAGGAAAT‑TCAAGGTTGTCGGCGGTTCTCT‑ACG‑CCG‑ACCGcccattgcac                                                                                                                                                                                            >  2:793489/1‑231 (MQ=255)

                                                 gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGGGAAAAA‑TCAGGGTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCCCCATTCGCGt                                                                                                                                                                                            >  1:1103793/1‑239 (MQ=255)

                                                 gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGt                                                                                                                                                                                            >  1:553286/1‑239 (MQ=255)

                                                                 tGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTccc                                                                                                                                                                             <  2:887086/238‑1 (MQ=255)

                                                                         aTTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGGAAAAATTCCCTACCGCTGa                                                                                                                                                                    >  1:29868/1‑239 (MQ=255)

                                                                           tgttGGCGGAAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGTTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGaaa                                                                                                                                                                  <  2:1174181/237‑1 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTCTCGTTTTCGAAGGGTTGCCGACCCGGCCGGACTGCGG‑CGT‑GTGGTGGGCAAACGTCGAGAAATA‑TAAGGGTAGCCGGATGTTCTCC‑GCG‑CCC‑ACCCCCCTTTGCGGGCTGAAAAAAATCCCTACCGCTGCAATTCGCac                                                                                                                                                           >  2:701620/1‑238 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGaaa                                                                                                                                                                                     <  2:73102/213‑1 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGaaa                                                                                                                                                                                     >  1:73102/1‑213 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                                                                                            <  1:985771/238‑1 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGGGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATTGCGACTCTCGTTTACCCAGGAATGCCGACCCTGCCGGACTGCGG‑CGT‑GTGGGGGGCCATCCGCGAGAGATA‑TCCGGTTAGCCGGGTGTTTTCC‑GCG‑CCG‑GCCCCCCTTCCCCGGCCGGAAAAATTCCCTACCGCTGGAATTCTCaa                                                                                                                                                           >  2:1084678/1‑239 (MQ=255)

                                                                                  ggcAAAGCGGGCAGCGTGTTCTTTTGCGCAGCGGATATCGGCTGGGTGGGGGGGCCTTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                                                                                            <  1:60135/238‑1 (MQ=255)

                                                                                              ggggttttCTTTTGCGCATCGGATACCGGCTGGGTGGGGGGGCATTCGTATTCCGTTTCCGGGCCGCTGCGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGCCCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                                                                                               <  2:1249094/233‑1 (MQ=255)

                                                                                                 gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                                                                                               <  2:448966/236‑1 (MQ=255)

                                                                                                 gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                                                                                               <  1:793489/236‑1 (MQ=255)

                                                                                                   gtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGc                                                                                                                                          <  1:1028603/239‑1 (MQ=255)

                                                                                                      cTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTgg                                                                                                                                       <  1:664675/239‑1 (MQ=255)

                                                                                                      cTTTTGCCCATCGGATTTCGGCTGGGGGGGGGGGCATTCGGATTGCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GGGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTgg                                                                                                                                       <  1:241300/239‑1 (MQ=255)

                                                                                                       ttttGCGCAGCGGATATCGGCGGGGTGGTGGGTCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGa                                                                                                                                      <  2:115180/239‑1 (MQ=255)

                                                                                                        tttGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATGGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGaa                                                                                                                                     <  1:804600/239‑1 (MQ=255)

                                                                                                            cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGAATGCGG‑CGT‑GTGGGGGACAATCGTCGGGAAATA‑TCAGGGTTGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTTCCTACCGCCGAAATTCTCACACACGAACCCTCGTCGCTGGAAGTg                                                                                                                                  >  2:348512/1‑238 (MQ=255)

                                                                                                            cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGGTTGCCGGCCTGGCCGGACTGCGG‑CGG‑GGGGTGGACAATCGTCGGGAAATA‑TTCGGGTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCCTTCGCCGGCTGAAAAAATTTCCTACCGCCTAAATTCGCCAACACGAtcctccgtccgtggaaagg                                                                                                                                  >  2:504674/1‑221 (MQ=255)

                                                                                                            cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGAATATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGt                                                                                                                                   >  1:115180/1‑237 (MQ=255)

                                                                                                                 cggaattCGGCTGGGTGGTGGGGCGCTCGATTATGGTTTACGCGCCGCTGCTGGGGGGGTTGGTGACTATCGTTTACGAAGGCTTGCCGCCGTGGCCGGGTTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAAAA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTAt                                                                                                                            <  2:955537/233‑1 (MQ=255)

                                                                                                                             gggTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGGCAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCCAACACGATCTCTCGTCGCTGGGAGGGCTCTATCTGGCTTgaga                                                                                                                 >  2:96249/1‑238 (MQ=255)

                                                                                                                             gggAGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGAGTATCGTTTAAGAAGGATTGCCGCACCGGCCGGGCTGCGG‑GGG‑GTGGGGGACAATCGGCGGGAAATA‑TCAGGGTTGCCGGCTGTTTTTC‑GGG‑GCG‑ACCGCCACTCCCGTGCTGACAAAATTCCCTACCCACGAAATTTGCAAACACGCACTCTCCTTGCTGGAAGTGCTTCATatggctgtcga                                                                                                                 >  2:63301/1‑234 (MQ=255)

                                                                                                                              ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTTGCGAATATCGTTTACGGAGGATTTGCGACCTGGCCGGAGTGCGG‑AGG‑GTGGGGGAAAAACGTCGAGAAATA‑TCAGGGTCGCCGGATGTTTTCA‑ACGCCCG‑ACC‑CCCTTAGCGGGGTCGAAAAATTTCCTTCCGCTGATAATTGCAAACATCATTTCTCGGCGCTGGactttcttctttctggatgggaa                                                                                                                >  2:1021081/1‑216 (MQ=255)

                                                                                                                              ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGCAGGATTGCCGGCCTCGCCGGACCGCGG‑CGG‑GGGGGGGGCAAACCTCGGGGAATACTCGGGTT‑GCCGGATTGTTTCA‑GCG‑CCGAACCGCCTTCCGCG‑GCTTGAAAAATTCCCTACCCGCGAAATTGGCCAACCCGATCTCCGCTCGCGTGAAGGGCTCTCTtcggctggggca                                                                                                                >  2:122710/1‑234 (MQ=255)

                                                                                                                              ggtgggGGGGCAGTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATct                                                                                                                          <  1:545029/228‑1 (MQ=255)

                                                                                                                              gggggtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACCGCGG‑GGG‑GTGGGGGGCAATCGTCGAGGAAAA‑TCAGGGTAGACGGGTGTTTTCA‑GCG‑CCG‑ACCGCCCTTAGCGTGCTGGAAAAAATCCCTACCGCTGAAATTCGCAAACACGAACTCTCGTCGCTGGAGGAGGCCTATctggcgtgggaa                                                                                                                >  2:517477/4‑231 (MQ=255)

                                                                                                                                  gtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCCAAACCGATCTCTCCTCGCTGGAAGTGCCCTATCTGGCTGGAGAAcc                                                                                                              >  1:358995/1‑236 (MQ=255)

                                                                                                                                   tggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGGTTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGGGTAGCCGGGTGTTCTCA‑GCG‑CCG‑GCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGcgg                                                                                                          >  2:266781/1‑237 (MQ=255)

                                                                                                                                        cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGGCAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAAAACGATCTCTCGGCGCTGGCAGTGCTCTATCTGGCTGGGGAAACGCTGGaggg                                                                                                     >  2:1169728/1‑236 (MQ=255)

                                                                                                                                               atgtCGTTTAGGGGCGGTTGCTGGGGGGGGTGGCGATTATCTTTTACGAGGGTTTCCGGGCTGGGCGGGCCTGCGG‑GGG‑GTGGGGGACAATCGTCGAAAAATA‑TCAGGTTAGCCGGATG‑TCTCAGGCC‑CGG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                                                                                               <  2:1043650/235‑1 (MQ=255)

                                                                                                                                               atatCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                                                                                               <  1:137418/238‑1 (MQ=255)

                                                                                                                                                tatCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAcc                                                                                              >  1:774453/1‑238 (MQ=255)

                                                                                                                                                 atCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTCTCGTTTTAGAAGGATTTCCGACCTGGCCGGACTGCGG‑CGT‑GGGGGGGACAATAGTCGAGAAATA‑TCAGGTTAGCCGGGTGGTCTCA‑GCG‑CCG‑GCCGCCATTCGCGGGCTGAAAAAATTCCCTACCCCTGGAATTCCCCAACACCATCTCTCGTCTGTGGAAGTGGCCCATCTGGGCGGTGAAACGCTGGGCGAGCCGcccc                                                                                             >  2:173509/1‑237 (MQ=255)

                                                                                                                                                  tCGTTTAGGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGCCCTGGCGGGACGGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGcc                                                                                           <  1:753080/239‑1 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGGGGGCGGCTATCGTTTACGAAGGATTGCCGACCCGGCCGGACTGCGG‑CGT‑GGGGGGGAAAATCGTCGAGAAATA‑TCAGGGTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGGGCTGAAAAAATTCCCTACCGCTGAAATTCCCAAACAACATCTCTCGTCGCTGGAAGTGCTCTCTCTGGCTGGGGAACCGCTGGACGCGCCCACCCCCCGt                                                                                        >  2:1256575/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCCTTCCCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGGTCTATCTGGCTTGAGGAACGCTGGACGAGCCGACCGCCAGt                                                                                        >  2:1407156/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAAACGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑AACGCCATTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGGCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCCGGAGGAGCCGACCCCCCGt                                                                                        >  1:1085762/1‑239 (MQ=255)

                                                                                                                                                     tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGGGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCA‑GGG‑CCG‑ACCGCCATTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGGGAACCGCTGGAAGAGCCGACCGCCa                                                                                          >  2:1323198/1‑237 (MQ=255)

                                                                                                                                                     ttgccgcgCCGCTGCTGGGGGGGGTGGCGACTATCGTTTACGAAGGATGGCCGCCCGGGCCGGACGGCGG‑CGG‑GGGGTGGACAACCGTCGAAAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                                                                                        <  2:1085762/235‑1 (MQ=255)

                                                                                                                                                       tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGGAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGAGCTCTATCTGGCTGGAGGACCGCTGGACGAGCCGACCGCCAGTTg                                                                                      >  2:1272689/1‑239 (MQ=255)

                                                                                                                                                       tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTAAGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑GCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGttt                                                                                      >  1:956813/1‑238 (MQ=255)

                                                                                                                                                        aCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCC‑GCG‑CCG‑AACGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGGAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGCCCCCCAGTTgg                                                                                     >  1:492781/1‑239 (MQ=255)

                                                                                                                                                        aCGCGCCGCTGCGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                                                                                     <  1:96249/239‑1 (MQ=255)

                                                                                                                                                          gcgcCGCTGCTGGGGGGGATGGCGATTATCGTTTAGGAAGGATTGCCGACCGGCCCGGACGGCGG‑CGT‑GTGGTGGACAATCGTCAAGAAATA‑TAAGGTTAGCCGGATGTCCTAA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                                                                                   <  2:38526/239‑1 (MQ=255)

                                                                                                                                                          gcgcCGCCGCTGGCGGGTGGGGCGTCTACCGTTTACGAGGGTGTGTCGACCTGGCGGGCCTGCGG‑GGT‑GTGGGGAACAATCTTCGAGAAATA‑CCAGGTTAGCGGGATGTCCCCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGAAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                                                                                   <  2:135206/239‑1 (MQ=255)

                                                                                                                                                           cgcggtggcggggggggAGGGCAATTTTGGTTTAGGAGGGTTGGCCGCCCTGGCCGGACGGGGG‑GGT‑GTGGTGGACAATCGTCGAGAAATA‑TAAGGTTAGCCGGATGTCCTCG‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                                                                                   <  2:1257606/225‑1 (MQ=255)

                                                                                                                                                             ccgcTGCTGGCGGGGATGGCGACTATCGTTTTCGAAGGATTGCCGACCTGGCCGGACTGCGG‑GGT‑GTGGTGGGCAATCGGCGAGGAATA‑TCAGGGTAGCCGGATGTTCTCA‑GCG‑CCC‑GCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACCCGATCTCCCCGCCCTGGAAATGCCGTATCTGGCTGGGGCACCGCCGGACGAGCCGCACGCCCCTTGGGTggg                                                                                >  2:489961/1‑237 (MQ=255)

                                                                                                                                                              cgcTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGG‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGGTAGCCGGGTGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCCATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGc                                                                               >  1:1257606/1‑239 (MQ=255)

                                                                                                                                                              cgcTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATTGCCGGCCCGGCCGGACCGCGG‑CGG‑GTGGTGGACAATCCTCGAGAAATA‑TCAGGTTTGCCGGGTCTTCTCA‑GCC‑CCC‑ACCGCCCTTCGCGTGCTGAAAAAATTCCCTACCCCTGAAATTCCCAAACACCAACCCTCGTCGCTGGTAGTGCCACATCCTGGTGGGGCACCGCTGGAAGAGCCGACCGccccatgggcgag                                                                                >  2:309226/1‑227 (MQ=255)

                                                                                                                                                                     gggggggTTGCCGACTGTCGTTAACGAAGGATGGCCGACCTGGCCGGGCTGGGG‑CGG‑GTGGTGGAGAATCGTGGAAAAAGT‑CCAGTTTAGCCGGATGTTCCCG‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg                                                                         <  2:898179/235‑1 (MQ=255)

                                                                                                                                                                              ggCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGAtgtg                                                                >  1:1302313/1‑238 (MQ=255)

                                                                                                                                                                                   tttgcGTTGAGGAAGGATTGCCGCCCTGGCCGGGCGGGGG‑GGT‑GGGGGGGCAAATGGGCGAGAAATA‑CCGGTTTAGCCGAATGTTCTCA‑GCG‑CGC‑ACCGCCATTCGCTTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGt                                                           <  2:1124115/234‑1 (MQ=255)

                                                                                                                                                                                         tttACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGAc                                                    >  2:107608/1‑239 (MQ=255)

                                                                                                                                                                                         ttaaCGATGGATTGCCGCCCTGGGCGGCCGGGGG‑CGG‑GGGGTGGAAAATCGTCGGAAAAAT‑TCGGGTTCGCCGGATGTTCTCA‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGa                                                     <  2:542603/235‑1 (MQ=255)

                                                                                                                                                                                            aCGAAGGATTGCCGCCCGGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAac                                                 <  1:1023230/239‑1 (MQ=255)

                                                                                                                                                                                             cGAAGGATTGCCCGCCCGGCCCGGATGTGGTCGT‑TGGGTGGAAATTCGCCGAAAAATT‑TCAGTTGACCCGGATGTTCCCG‑GCC‑CCA‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAac                                                 <  2:1150902/239‑1 (MQ=255)

                                                                                                                                                                                                aGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGGCAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  2:1402110/1‑236 (MQ=255)

                                                                                                                                                                                                aGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  1:228181/1‑236 (MQ=255)

                                                                                                                                                                                                    ttGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGCGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACCACTACTGGCa                                         >  1:581044/1‑239 (MQ=255)

                                                                                                                                                                                                          aCCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAact                                                >  1:1197941/1‑226 (MQ=255)

                                                                                                                                                                                                            cTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGaa                                  >  2:142418/1‑238 (MQ=255)

                                                                                                                                                                                                            cTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGGGCCGGTCATCGACAACTACTGGCAGACCGaa                                  >  2:381887/1‑238 (MQ=255)

                                                                                                                                                                                                              ggCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACCGGCAGACCGAATc                                >  2:667997/1‑238 (MQ=255)

                                                                                                                                                                                                               gCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCg                              >  1:549310/1‑239 (MQ=255)

                                                                                                                                                                                                                    aCTGCGG‑CGT‑GGGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGTATGTCCTCA‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTg                          <  1:173509/238‑1 (MQ=255)

                                                                                                                                                                                                                     cggcgg‑cgT‑GGGGTGGACAACCGGCGAAAAATA‑TCAGGTTGGCCGTATGTTCCCA‑GCG‑CAG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACTATCTCTCGTCGCTGAAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                        <  1:1021081/237‑1 (MQ=255)

                                                                                                                                                                                                                     cTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                        <  1:107608/239‑1 (MQ=255)

                                                                                                                                                                                                                        ggcggctgtgggtggAAAACCGGCGAGAAATT‑TCGGGTTGCCCGGATGTTCCAA‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAGCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGc                        <  2:358995/228‑1 (MQ=255)

                                                                                                                                                                                                                                     ggaaaacggtggagaAATA‑TCAGGTTAGCCGGATGTTCCCA‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGAt           <  2:1372326/227‑1 (MQ=255)

                                                                                                                                                                                                                                         aaTCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGCTTATGGCGATTGCTc      >  1:975891/1‑239 (MQ=255)

                                                                                                                                                                                                                                          agcgtcgAAAAATA‑ACAGGTTAGCCGGATGTCCTCA‑GCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcg     <  2:553286/237‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCGTCGAGAAATA‑TCAGTTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcg     <  1:348512/239‑1 (MQ=255)

                                                                                                                                                                                                                                           tcgtcgAGAAATA‑TCATGAAAAACGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGGGCTGAAAAAATACACTACCGCTGAAATTCGCAACCACGATCTCTCGCCGCTGGAGATGCTCTATCTGGATGGAGAACCGCTGGACGTGCGGACCGCCGCGTGGCTGAGCAATCCGCTGGATGTGCCGGTCATCGACAACGACAGGCAGACCGAAGACTGCTGGCCGATTCTGGCCATGGCTcgc    >  2:837985/1‑239 (MQ=255)

                                                                                                                                                                                                                                           tcgtcgAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTCTGGCGATTGCTcgc    >  1:997650/1‑239 (MQ=255)

                                                                                                                                                                                                                                           tcgtcgAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgc    >  1:900322/1‑239 (MQ=255)

                                                                                                                                                                                                                                           tcgtcgAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgc    >  1:1062472/1‑239 (MQ=255)

                                                                                                                                                                                                                                            cgtcgAAAAAGA‑CCGGGTTGGGCGGTTTTTCCCA‑GCC‑CCG‑CCCGCCATTGGCGTTCTGAAAAAATTCCCTACCGCTGAAATCCCAAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCGGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg   <  2:774453/239‑1 (MQ=255)

                                                                                                                                                                                                                                             gtcgAGAAATA‑GCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGAAAGGGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTcgcg   <  1:517477/238‑1 (MQ=255)

                                                                                                                                                                                                                                              tcgAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCgg  >  1:1226836/1‑238 (MQ=255)

GACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGG‑CGT‑GTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCA‑GCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGG  >  NC_000913/353434‑353907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`