BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,256,052	G→C	11.4%	G138G (GGC→GGG)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,256,052	0	G	C	11.4%	348.3 / 3.7	127	G138G (GGC→GGG)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand): ref base G (29/78); new base C (10/5); total (42/85)
Fisher's exact test for biased strand distribution p-value = 5.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

ATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGAC  >  NC_000913/4255821‑4256287

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTTACTT‑CACCctttt                                                                                                                                                                                                                                             >  1:3553/1‑239 (MQ=255)

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCCATT‑CGCCttttt                                                                                                                                                                                                                                             >  1:796402/1‑239 (MQ=255)

aTCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCtttt                                                                                                                                                                                                                                              >  1:17211/1‑238 (MQ=255)

 cccTGACGGGCAGGCAAACGGGGCCCTGCGGCGGCCAGACGTCGACGGG‑AAAGGTGCATCCGCGCCACGCGCGCCAGTTTCTGGGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCttttt                                                                                                                                                                                                                                             <  2:449745/237‑1 (MQ=255)

  ccTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CCCCTTTTTc                                                                                                                                                                                                                                            >  1:747739/1‑238 (MQ=255)

  ccTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTc                                                                                                                                                                                                                                            >  1:277449/1‑238 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCCCCT‑TCCCTTTTTTCCGccccc                                                                                                                                                                                                                                    >  1:194048/1‑237 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTTACCT‑CGCCCTTTTCACGcccccg                                                                                                                                                                                                                                   >  2:47626/1‑237 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCCACT‑CCCCTTTTTTAccccccc                                                                                                                                                                                                                                    >  2:644486/1‑237 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTCCGccccc                                                                                                                                                                                                                                    >  1:1056035/1‑237 (MQ=255)

          gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCCTTTTTCCGccccc                                                                                                                                                                                                                                    >  1:1083851/1‑237 (MQ=255)

           cAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACAT‑CGCCttttttcacccccgg                                                                                                                                                                                                                                   >  2:830979/1‑228 (MQ=255)

               cAAGCGTGGGCCTCCGGCAGCCGACCGTTGACGGG‑CAAAGTGCGTCCGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTACCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑Ggcgcg                                                                                                                                                                                                                              <  2:756574/238‑1 (MQ=255)

               cAACCGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGGGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑Ggcgcg                                                                                                                                                                                                                              <  2:436453/238‑1 (MQ=255)

                 agcGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑a                                                                                                                                                                                                                         <  2:783848/237‑1 (MQ=255)

                 aaCGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑a                                                                                                                                                                                                                         <  1:1053262/239‑1 (MQ=255)

                      gggCCTACGCCAGCCAGACGTTGACGGG‑CAAGGTGCATCCGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCaa                                                                                                                                                                                                                     <  2:476600/238‑1 (MQ=255)

                        gCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTTACTTCCACTTTTTTCAC‑CCCC‑CGCGCCC‑G‑CCCaaaac                                                                                                                                                                                                                  >  1:1195865/1‑237 (MQ=255)

                        gCCGACGGCAGCCAGACGTTGACGGG‑CAAAGGCCATACGCGCCACGCGCCCCAGTTTCGGAGCG‑ATAGTTTTACCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAAc                                                                                                                                                                                                                   <  1:738740/238‑1 (MQ=255)

                            aCGGCAGCCAGACGTTGACGGG‑CAAAGTGATTACGCGCCTCGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCTTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAc                                                                                                                                                                                                              <  1:158905/239‑1 (MQ=255)

                             cGGCAGCCAGACGGTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCTACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                             <  1:994991/239‑1 (MQ=255)

                              gcgaccgcgcgttggACGGG‑CAAAGTGCATCCGCGCCCCCCGGCCCAGTTTTTG‑GCGAATATTTTTATCCGTGCCGTG‑TTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                             <  2:1225224/224‑1 (MQ=255)

                              gccAGCAAGACGTGGACGGG‑CAAAGTGCATCCGCGCCCCGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCg                                                                                                                                                                                                            <  2:485693/237‑1 (MQ=255)

                              cggcaccAGGCGGTGGCGGGCCAAGGTGCACGCGCGCCTCGCGCGCCTGTTTCTGAGCG‑ATAGTTTTACCCGGGCCGTT‑TTCATCCGCCATACGGCGCAGCGAAACCGACGGCGAGAAACGCACAAAACTGCCGGGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                             <  2:889632/234‑1 (MQ=255)

                              cgcgacccagaggtggcgggacAAAGTGCATACGCGCCGCGCGCCCCAGTTTGTGAGCG‑ATAGTTTTACCCGTGCCGTG‑TTCATCCCCCATCCGGCGCAGCGAAACGGACGGCGAGAACCGCACAAATCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                             <  2:1274639/218‑1 (MQ=255)

                                cAGCCAGGCGTTAAGGGG‑CAATGGAAATACGCCCCACGCGCCCCAGTTTCGGAGCG‑ATAGTTTATCCCGTGCCGGG‑TTCACCCTCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                             <  2:667726/236‑1 (MQ=255)

                                     aGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTCCGCCCC‑CGCGCCC‑G‑GCCCAAAATCAccccacccg                                                                                                                                                                                                      >  1:7710/1‑231 (MQ=255)

                                       aCGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑c                                                                                                                                                                                                   <  1:81683/238‑1 (MQ=255)

                                             aCGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AACCATACGCAGCGGC‑GGGGTCACCT‑CGCCTTTTTTACCCCCC‑CTTGCGC‑C‑ACCAAACATACCCGACCCAGG‑cccccac                                                                                                                                                                                             >  1:1293280/1‑235 (MQ=255)

                                                           aTACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑Att                                                                                                                                                                              <  1:252704/239‑1 (MQ=255)

                                                                cgcCCCGCGCGCCAGTTTCGGGGCG‑ATAGTTTTAGCCGGGCCGTT‑TTCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGt                                                                                                                                                                         <  2:533516/239‑1 (MQ=255)

                                                                ccccACGCGCCCCAGTTTCGGAGTG‑GTATTTTAGCCCGCGCCGTG‑TTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGt                                                                                                                                                                         <  2:171289/237‑1 (MQ=255)

                                                                 cccACGCGCGCCAGTTTCTGAGAG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGt                                                                                                                                                                         <  1:26206/237‑1 (MQ=255)

                                                                  ccACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑TGCCTTTTTTACGCCCC‑GGCGCCG‑G‑CACAAAAATCACCGCCAATAG‑CACCATACGCACAAACAGAATTTGGGt                                                                                                                                                                         >  1:1242075/1‑238 (MQ=255)

                                                                   caggcgcgcCAGTTTCTGACCA‑ATAGTTTTATCCGTGCCTTG‑TTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACCCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGc                                                                                                                                                                      <  1:944215/236‑1 (MQ=255)

                                                                    ccgcgcgCCGGTTTCTGCGTG‑ATGGTTTTATCCGCCCCGTT‑CACAGCCCCCATACGGCGCAGCGAAACGCACGGCGAGAAACCCCCAAAACTGGCCCGCCCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑CCCAAACATCACCGACACTGG‑CGCCATCTGCACATCCAG‑ATTTGGGTTGC‑t                                                                                                                                                                    <  2:447032/238‑1 (MQ=255)

                                                                     cgcggcccgtttgcggAGCG‑TTGGTTTTATCGTGGCGGTT‑TTCCTCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAGCGCACAAAACTGTCCCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGGTCG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑Ta                                                                                                                                                                   <  2:148856/224‑1 (MQ=255)

                                                                     cgcgccCCAGTTTCGGAGCA‑ATATTTTTACCCGGCCCTTG‑TTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑Ta                                                                                                                                                                   <  1:1395762/239‑1 (MQ=255)

                                                                      gcgggcCATTTTCCGAGCG‑TTAGTTTTACCCGGGCCGTT‑TCCACCCCCCATACGGCGCAGGGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TAc                                                                                                                                                                  <  2:1056035/239‑1 (MQ=255)

                                                                          gcCAGTTTCTAGGCG‑TTATTTTTATCCGTGCCCGG‑TCCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGACGCGCACGTGCCACCGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑ACTCATACGACGCGGC‑GGGTTCACTC‑CGCGTTTTTCCCGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGTTg                                                                                                                                                              <  2:1293280/239‑1 (MQ=255)

                                                                          gcCAGTTGCTGAGCG‑ATAGTTTTACCCGTGCGGTG‑TTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTg                                                                                                                                                              <  2:369910/239‑1 (MQ=255)

                                                                                  cgggggg‑ATAGTTTTATCCGGGCCTGG‑TTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCga                                                                                                                                                      <  2:635477/233‑1 (MQ=255)

                                                                                  cTGAGCT‑ATAGTTTTACCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCga                                                                                                                                                      <  1:47626/239‑1 (MQ=255)

                                                                                    tggtcgttagtttgcgccgtgccgtc‑TACATCCGCCATACGGCGCAGCGAACCTGATGTCGAGAACCGCACAAACCTGCCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCCCGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑AGTTGGGTTGC‑TACGGTGCAAATCgag                                                                                                                                                     <  2:1217568/223‑1 (MQ=255)

                                                                                      gCG‑TTAGTTTTACCCGGGCCTGG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  2:1103739/238‑1 (MQ=255)

                                                                                      gCG‑ATAGTTTTGCCCGGGCCGTG‑TGCAGCCCCCATAGGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACTGTCGCCACCGAGCCGCAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  2:179940/238‑1 (MQ=255)

                                                                                      gCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  1:830979/238‑1 (MQ=255)

                                                                                      gCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  1:1048743/238‑1 (MQ=255)

                                                                                      gCG‑ATAGTTTTACCCGTGCCGTG‑TTCCACCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  2:277449/238‑1 (MQ=255)

                                                                                      gCG‑AGAGTTTTACCCGTGCCGTG‑TTCCTCCGCCATACGGCGCAGCGAAACTGACGCCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTCCG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  1:958708/238‑1 (MQ=255)

                                                                                       ccgagagttttaccggcgcgtgg‑tTCACCCGCCATCCGCCGCAGCGAAACTGACGGCAAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  2:792208/216‑1 (MQ=255)

                                                                                               tttCTCCGTGCCGTT‑TTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                           <  2:1377677/238‑1 (MQ=255)

                                                                                               tttATCCGTGCCGTT‑TTCAACCGCGATACGGCGCAGAGAAACTGACGGGGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                           <  1:385850/238‑1 (MQ=255)

                                                                                                ttATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  1:536248/239‑1 (MQ=255)

                                                                                                ttATCCGTGCAGTT‑TCCAACCGCCATCCGGCGCAGCGAAACGGACGGCGAGAAACGCCCAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  1:644486/239‑1 (MQ=255)

                                                                                                ttATCCGCGCCTTG‑TCCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  2:607770/239‑1 (MQ=255)

                                                                                                ttATCCG‑GCCCTGTTTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  2:302091/239‑1 (MQ=255)

                                                                                                ttAGCCGGGCCGTG‑TTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  2:544950/239‑1 (MQ=255)

                                                                                                ttACCCGTGCCGTT‑TCCATCCGCCAACCGGCGCAGCGAAACTGACGGCGAGAACCGCACAAAACGGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  2:1382619/239‑1 (MQ=255)

                                                                                                taagccgggccgtg‑TTCATCCGCCATACGGCGAAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                         <  1:579885/235‑1 (MQ=255)

                                                                                                       tgccgtg‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGCGGGGTCCACTT‑‑GCCTTTTTTTCCGCCC‑CGCGCCC‑C‑GCCAAACACCACCCACCCTGGCCCCCACCTCCCCA‑CCAA‑ATTTTGGGTGC‑TTCGGTGCAAAACgaaaatagcggggaaaagcttt                                                                                                                                  >  2:1306597/1‑219 (MQ=255)

                                                                                                       tgccgtg‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑CGCGCGC‑G‑ACCCAACATCACCCACACTGG‑CCCCATCTGCACCTCCAG‑ATTTGGGGTGC‑TTCGGGGCAAATCGAGATAATGGTGGAACAGC‑ttc                                                                                                                                 >  2:62231/1‑238 (MQ=255)

                                                                                                       tgccgtg‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTTACTT‑CGCCCTTTTCACGCCCC‑GGCGCGC‑G‑GCCAAACAACACCGACACCGG‑GCCCCTCCGCCCATACCG‑CTTTGGGTTGC‑TACGGGGCCAAACgagagaattcgtggacaagcta                                                                                                                                  >  2:170369/1‑221 (MQ=255)

                                                                                                        gccgtg‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑TCCCCTTTTTACGCCCC‑GGCCCGC‑G‑GCCAAAAATCACCCACCCTTG‑CACCATCTTCCCCTCCAG‑ATTTTGGTTGC‑TTCGGGGCAAATTCAGATAGTCGTGGGACAGC‑TTaa                                                                                                                                >  2:929709/1‑239 (MQ=255)

                                                                                                        gccgtg‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CCCCTTTTTTACGCCCC‑GGCGCGC‑G‑ACCAAACAACACCGACACCGG‑CACCATCTCCACAACCAG‑ATTTGGGGTGC‑TACGGTGCAAATCGGGATAGTCGTGGAAAAGC‑TTaa                                                                                                                                >  1:834845/1‑239 (MQ=255)

                                                                                                        gccgtg‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CCCCTTTTTCACCCCCC‑CGCGCGC‑G‑CCCCAACACCACCCACCCCGG‑CACCATCTTCACCTCCCA‑CTTTGGGGTGC‑TACGGTGCCAATCGAGATAATCGTGGAAAAGC‑TTaa                                                                                                                                >  2:1292722/1‑239 (MQ=255)

                                                                                                            tg‑tTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑Ggcgcg                                                                                                                                                                                                                              <  1:466285/147‑1 (MQ=255)

                                                                                                                  aTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑TCCCCTTTTTACGCCCC‑CGCGCGC‑G‑ACCAAACAACCACCACACTGG‑CCCCCTCTGCCCACCCAG‑ACTTTGGTTGG‑CACGGTGCAAATCGAGATAGTCGTGGAAACGC‑TTaaaataatct                                                                                                                        >  2:117224/1‑230 (MQ=255)

                                                                                                                      gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCttt                                                                                                                   <  1:274719/239‑1 (MQ=255)

                                                                                                                       ccATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CTCCTTTTTTACCCCCC‑CGCGCGC‑G‑Accccaca                                                                                                                                                                                                                  >  2:466285/1‑142 (MQ=255)

                                                                                                                        cATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCttt                                                                                                                   <  1:1069791/237‑1 (MQ=255)

                                                                                                                        cATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCg                                                                                                                 <  1:170369/239‑1 (MQ=255)

                                                                                                                         atccGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                <  1:1292722/236‑1 (MQ=255)

                                                                                                                         aTACGGCGCAGCGAAACTGACGGCCAGAACCGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                <  1:1038953/239‑1 (MQ=255)

                                                                                                                         aTACGGCCAAGCGAAACGGACGGAGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                <  2:747739/239‑1 (MQ=255)

                                                                                                                           aCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCg                                                                                                              <  2:1111227/239‑1 (MQ=255)

                                                                                                                            cGGCGCAGCGAAACGGACGGCGAGAAACGCAAAAAACGGCCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                <  2:775400/236‑1 (MQ=255)

                                                                                                                               cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                   <  1:206756/199‑1 (MQ=255)

                                                                                                                               cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCgtgt                                                                                                           <  1:62231/238‑1 (MQ=255)

                                                                                                                                gcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACACTTCTTTCGGCGTGTaa                                                                                                         <  1:516048/239‑1 (MQ=255)

                                                                                                                                 cAGCGAAACTGACGGCGAGAAACGCCCAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTCCG‑CCGTT‑AAGCATACGCAGCGGC‑GGTTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAAt                                                                                                        <  1:885248/239‑1 (MQ=255)

                                                                                                                                  aGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCCCTT‑CCCCTTTTTCACCCCCC‑GGCGCAC‑C‑ACCAAAAAACACCGACCCCCG‑CGCCCTCTGCACCTCCAG‑ATTTTGGGTGC‑TACGGCGTAAAATGCAAAAGTAGTGGAACAACGTTAATAG‑CTTTTCtttttcgctggaatt                                                                                                       >  2:1069791/1‑227 (MQ=255)

                                                                                                                                    cGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCCTTTTTACCCCCC‑GGCGCGC‑G‑GCCCAACAACACCGACCCTGG‑CACCATCCGCACATCCCG‑ATTTTGGTTGC‑TACCGGGGAAATCGAGATAGTCGTTGACCAGC‑TTAATAGACTCTTCTTTCGGCGTGGAATCgg                                                                                                     >  2:40057/1‑239 (MQ=255)

                                                                                                                                    cGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTACGCCCC‑CGCGCGC‑G‑ACCAAACAACCCCGACACAGG‑CACCCTCTGCACATCCAG‑ATTTTGGGTGC‑TACGGAGGAAATCGAGATAGACGGGGGACAGC‑GTAATAAACTCTTTTTTTGGCCGTTAATAgg                                                                                                     >  2:1301028/1‑239 (MQ=255)

                                                                                                                                        acgaaCGGCGAGAAACGCACAAAACTGTCGGGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGGGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGaa                                                                                                 <  2:194048/235‑1 (MQ=255)

                                                                                                                                         cTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTACGCCCC‑CGCGCGC‑G‑ACCAAACATCACCGACACTCG‑CCCCATCTGCACACCCCG‑ATTTGGGTTGC‑TACGGTGCAAAACGAGATAGTCGTGGaa                                                                                                                                         >  2:206756/1‑199 (MQ=255)

                                                                                                                                                         cacaAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTTACGCCCC‑GGCGCGC‑G‑ACCAAAAATCCACCAAACCGG‑AACCATCTCCCCCTTCCG‑ATTTTGGTTGG‑TAACGTGGAAAACGAGGTAGTCCTGGGAGCGA‑TTAATATAACTTTCTTTCTGCGGCTGATATATGGAAACAAGCGGCccgccgg                                                                                >  2:103021/1‑238 (MQ=255)

                                                                                                                                                                 tGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCttt                                                                                                                   <  1:1191882/196‑1 (MQ=255)

                                                                                                                                                                       gACCGAGCCACAGTACAGCAAAAAA‑TTTCTGTCCG‑CCGTT‑AAACATACGCAGCGGC‑GGTTTCCCTT‑CCCCTTTTTCCCCCCCC‑GGCGCGC‑G‑ACAAAACATCCCCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGGGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAg                                                                  <  1:378013/239‑1 (MQ=255)

                                                                                                                                                                        gaccgagcaaaacaccagcaaaaa‑TTTCTTGCCGCCCTTT‑AACCAT‑CGCAGCGGG‑GGGTTTCCCT‑CCCCTTTTCCCCCCCCCGGGCGCGC‑A‑ACAAAACACCCCCGACACGGG‑CACCATCTGCACATCCAG‑TTTTGGGTTGC‑TAGGGTAAAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAg                                                                  <  2:1365607/220‑1 (MQ=255)

                                                                                                                                                                        aCCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCCACACTGG‑CACCCTCTGCACATCCAG‑ATTTTGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTTATAGACTCTTCTTTCTGCGTGTAATAGGTGAAAACACGCGGCCCGCCGTGAATGAACAAATAg                                                                  >  1:210654/1‑238 (MQ=255)

                                                                                                                                                                        aCCGAGCCACAGTAC‑GAGAAAAATTTTTTGGCCG‑CCGTT‑AACCATACGCAGCGGC‑GGGTCCACTT‑CCCTTTTTCCCCGCCCC‑GGCGCGA‑G‑TACAAACAACCCCACCGCTGA‑CCCCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTACAAATCGGGATAGTCGGGGAACAGA‑TTAATAACCTCTTCTTTCGGCGTTTAATAGGTGACCACCCGCGGCCCGCCGTGAATGAACACATAg                                                                  <  2:866010/238‑1 (MQ=255)

                                                                                                                                                                         cggcccccaggtccggagAAAAA‑TTTCT‑TACC‑CCCTTAAAGCTTCCCCGCCGGG‑GGTTTCATTC‑CGCTTTTTCCCCGCCCC‑GGCCCGC‑G‑CCCAACAACCACCGACACTGC‑CACCATCTGCACATCCAG‑ATTTGGTTTGC‑TACGGTCCAAATGGAGATAGGCGTGGAACAGC‑TAAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAgcg                                                                <  2:1268869/222‑1 (MQ=255)

                                                                                                                                                                         ccgagcgcgattACAGCAAAAAA‑TTCTTGTGCG‑CGTTT‑AGGACTACGCAGGGGG‑GTGTTCCCTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAAACTCACCGACACGGG‑CACCATCTGCACATCAAT‑ATTTGGGTTGC‑TACGGTGCAAATAGAGATAGTCGGGGAACAGC‑TTAATAGACTCTTCTTCCGGCGTTTAATAGGCGAACACACGCGCCCCGCCGTGAATGAACACATAgcg                                                                <  2:899618/228‑1 (MQ=255)

                                                                                                                                                                                                         cG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTTACGCCCC‑CGCGCGC‑G‑ACCCAACATCACCGACACCGG‑CCCCATCTGCCCATCCCG‑ATTTGGGTTGC‑TACGGTGCAAATCCAGATAGTCGTGGGACAGC‑TTAATAGACTCTTCTTTCTGCGTGTAATAGGTGAACACACGCGGCCCGCCGCGAATTAAAACATAACGCGGCAGGAGCGGGCCGTCGATTTCCCGCgg                                  >  2:920172/1‑238 (MQ=255)

                                                                                                                                                                                                                 taggcacgcgccgcGGC‑GCGTTCACTC‑CGCCTTTCCCACGCCCG‑GGCGCCC‑G‑ACAAAACATCACCGACACTGC‑CCCCATCTCACCACCCGG‑ATTTGGTTTGC‑TACGGGGCAAATCGAGATGGTCGTGGAACAGC‑TAAAAAGACTCTCCTTTCGGCGTGTAATAGGGGAACACCCGCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTaa                            <  2:19322/230‑1 (MQ=255)

                                                                                                                                                                                                                     ccacggaggcggc‑gGGTCGCCTT‑CTCC‑TTTGCCCGCCCC‑GGCGCGCGG‑ACCAAACACCCCCGACACTGG‑CACCATCTGCCCATCCAG‑TTTTGGGTTGC‑TACGGGGCAAAGCGAGATAGTCGTGAAACGGC‑TAAATAGACTCTTCTTGCGGGGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                       <  2:916014/231‑1 (MQ=255)

                                                                                                                                                                                                                                 c‑ggGGTCACTT‑CGCCTTTTTTACGCCCC‑GGCGCGC‑G‑ACCCAACCTCACCGACACTGG‑CCCCATCTGCACATCCAG‑ATTTTGGTTGC‑TACGGGGCAAATCGAGATAGTCGTGGGACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCCGTAGCGGGCCGTCGGTTTCCAGCGGCCCTAACCGGGCAGGGCAGTCAt           >  2:686926/1‑239 (MQ=255)

                                                                                                                                                                                                                                    tgTTCCCCT‑CCCCCTTTTCCCCCCCC‑GGGCGGC‑GCACCAAACCCCCCCGACACTGG‑CACCATCTGCACTCCCAG‑‑TTTGGGTTTCGTGCGGTGCAACTCGAGATAGTCGGGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGGGACCCCCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAt           <  2:796402/237‑1 (MQ=255)

                                                                                                                                                                                                                                         aCTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTaa                                                                                                         >  1:5304/1‑138 (MQ=255)

ATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCG‑ATAGTTTTATCCGTGCCGTG‑TTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTCTGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACATCCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGAC  >  NC_000913/4255821‑4256287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`