BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,256,061	A→C	10.5%	R135R (CGT→CGG)	plsB ←	glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,256,061	0	A	C	10.5%	292.9 / 2.1	122	R135R (CGT→CGG)	plsB	glycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand): ref base A (29/74); new base C (6/11); total (36/86)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

GCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACA  >  NC_000913/4255831‑4256288

gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCCCCT‑TCCCTTTTTTCCGccccc                                                                                                                                                                                                                                      >  1:194048/1‑237 (MQ=255)

gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTTACCT‑CGCCCTTTTCACGcccccg                                                                                                                                                                                                                                     >  2:47626/1‑237 (MQ=255)

gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCCACT‑CCCCTTTTTTAccccccc                                                                                                                                                                                                                                      >  2:644486/1‑237 (MQ=255)

gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTCCGccccc                                                                                                                                                                                                                                      >  1:1056035/1‑237 (MQ=255)

gCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCCTTTTTCCGccccc                                                                                                                                                                                                                                      >  1:1083851/1‑237 (MQ=255)

     cAAGCGTGGGCCTCCGGCAGCCGACCGTTGA‑CGGG‑CAAAGTGCGTCCGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTACCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑Ggcgcg                                                                                                                                                                                                                                <  2:756574/238‑1 (MQ=255)

     cAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑Ggcg                                                                                                                                                                                                                                  <  1:124035/236‑1 (MQ=255)

       aaCGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑a                                                                                                                                                                                                                           <  1:1053262/239‑1 (MQ=255)

            gggCCTACGCCAGCCAGACGTTGA‑CGGG‑CAAGGTGCATCCGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCaa                                                                                                                                                                                                                       <  2:476600/238‑1 (MQ=255)

              gCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTTACTTCCACTTTTTTCAC‑CCCC‑CGCGCCC‑G‑CCCaaaac                                                                                                                                                                                                                    >  1:1195865/1‑237 (MQ=255)

                  aCGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGATTACGCGCCTCGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCTTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAc                                                                                                                                                                                                                <  1:158905/239‑1 (MQ=255)

                   cGGCAGCCAGACGGTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCTACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                               <  1:994991/239‑1 (MQ=255)

                    cggcaccAGGCGGTGG‑CGGGCCAAGGTGCACGCGCGCCTCGCGCGCCTGTTTCTGAGCGA‑TAGTTTTACCCGGGCCGT‑TTTCATCCGCCATACGGCGCAGCGAAACCGACGGCGAGAAACGCACAAAACTGCCGGGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                               <  2:889632/234‑1 (MQ=255)

                     cgcgacccagaggtggcgggacAAAGTGCATACGCGCCGCGCGCCCCAGTTTGTGAGCGA‑TAGTTTTACCCGTGCCGT‑GTTCATCCCCCATCCGGCGCAGCGAAACGGACGGCGAGAACCGCACAAATCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                               <  2:1274639/218‑1 (MQ=255)

                      cAGCCAGGCGTTAA‑GGGG‑CAATGGAAATACGCCCCACGCGCCCCAGTTTCGGAGCGA‑TAGTTTATCCCGTGCCGG‑GTTCACCCTCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCAcc                                                                                                                                                                                                               <  2:667726/236‑1 (MQ=255)

                        gCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CCGCTTTTTTCCCCCCC‑GGCGCGC‑G‑ACCAAACATCACCcaca                                                                                                                                                                                                           >  1:533516/1‑238 (MQ=255)

                           cggagTTGACCGGG‑CAAGGTGCATGCGCGCCCCGCGCGCAGGTTTCTGGGGGA‑TAGTTTTTCCCGTGCCGG‑GTTCACCGCCCATACGGCGCAGCGAAACTGACAGCGAGAAACGCACAAAACTGTCGCGACCGAGCCCCAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACAGCACCGaca                                                                                                                                                                                                           <  2:910754/232‑1 (MQ=255)

                           aGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTCCGCCCC‑CGCGCCC‑G‑GCCCAAAATCAccccacccg                                                                                                                                                                                                        >  1:7710/1‑231 (MQ=255)

                             aCGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑c                                                                                                                                                                                                     <  1:81683/238‑1 (MQ=255)

                              cGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑c                                                                                                                                                                                                     <  1:339893/237‑1 (MQ=255)

                                   a‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AACCATACGCAGCGGC‑GGGGTCACCT‑CGCCTTTTTTACCCCCC‑CTTGCGC‑C‑ACCAAACATACCCGACCCAGG‑cccccac                                                                                                                                                                                               >  1:1293280/1‑235 (MQ=255)

                                     gggg‑CAAGGGGCATCCGCGCCCGGCGCGCC‑GTTTTTGGACGACAAGTTTAACCCGT‑CCGTGGTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTCAAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAACCATCACCGACACTGG‑CACCATCTg                                                                                                                                                                                             <  2:821723/238‑1 (MQ=255)

                                     cGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTg                                                                                                                                                                                             <  1:1060790/239‑1 (MQ=255)

                                                  aTACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑Att                                                                                                                                                                               <  1:252704/239‑1 (MQ=255)

                                                   tACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCCCCCCCC‑CGCGCGC‑C‑ACCCAAAATCACCGCCACTCG‑CACCACCTGCCCAA‑CCCG‑Agtt                                                                                                                                                                              >  1:783451/1‑239 (MQ=255)

                                                       cgcCCCGCGCGCCAGTTTCGGGGCGA‑TAGTTTTAGCCGGGCCGT‑TTTCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGt                                                                                                                                                                          <  2:533516/239‑1 (MQ=255)

                                                       ccccACGCGCCCCAGTTTCGGAGTGG‑TATTTTAGCCCGCGCCGT‑GTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGt                                                                                                                                                                          <  2:171289/237‑1 (MQ=255)

                                                         ccACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑TGCCTTTTTTACGCCCC‑GGCGCCG‑G‑CACAAAAATCACCGCCAATAG‑CACCATACGCACAA‑ACAGAATTTGGGt                                                                                                                                                                          >  1:1242075/1‑238 (MQ=255)

                                                          caggcgcgcCAGTTTCTGACCAA‑TAGTTTTATCCGTGCCTT‑GTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACCCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGc                                                                                                                                                                       <  1:944215/236‑1 (MQ=255)

                                                           ccgcgcgCCGGTTTCTGCGTGA‑TGGTTTTATCCGCCCCGT‑TCACAGCCCCCATACGGCGCAGCGAAACGCACGGCGAGAAACCCCCAAAACTGGCCCGCCCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑CCCAAACATCACCGACACTGG‑CGCCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑t                                                                                                                                                                     <  2:447032/238‑1 (MQ=255)

                                                            cgcggcccgtttgcggAGCGT‑TGGTTTTATCGTGGCGGT‑TTTCCTCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAGCGCACAAAACTGTCCCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGGTCG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑Ta                                                                                                                                                                    <  2:148856/224‑1 (MQ=255)

                                                            cgcgccCCAGTTTCGGAGCAA‑TATTTTTACCCGGCCCTT‑GTTCATCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑Ta                                                                                                                                                                    <  1:1395762/239‑1 (MQ=255)

                                                             gcgggcCATTTTCCGAGCGT‑TAGTTTTACCCGGGCCGT‑TTCCACCCCCCATACGGCGCAGGGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TAc                                                                                                                                                                   <  2:1056035/239‑1 (MQ=255)

                                                                 gcCAGTTTCTAGGCGT‑TATTTTTATCCGTGCCCG‑GTCCACCCGCCATCCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGACGCGCACGTGCCACCGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑ACTCATACGACGCGGC‑GGGTTCACTC‑CGCGTTTTTCCCGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGTTg                                                                                                                                                               <  2:1293280/239‑1 (MQ=255)

                                                                 gcCAGTTGCTGAGCGA‑TAGTTTTACCCGTGCGGT‑GTTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTg                                                                                                                                                               <  2:369910/239‑1 (MQ=255)

                                                                 gcCAGTTGCGGGGCGA‑TGGTTTTGCCCGGGCCGT‑GTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTCCG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTg                                                                                                                                                               <  2:684092/239‑1 (MQ=255)

                                                                       ttCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAAt                                                                                                                                                          <  1:130875/238‑1 (MQ=255)

                                                                         cggggggA‑TAGTTTTATCCGGGCCTG‑GTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCga                                                                                                                                                       <  2:635477/233‑1 (MQ=255)

                                                                         cTGAGCTA‑TAGTTTTACCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACGGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCga                                                                                                                                                       <  1:47626/239‑1 (MQ=255)

                                                                           tggtcgttagtttgcgccgtgccgt‑cTACATCCGCCATACGGCGCAGCGAACCTGATGTCGAGAACCGCACAAACCTGCCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCCCGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑AGTTGGGTTGC‑TACGGTGCAAATCgag                                                                                                                                                      <  2:1217568/223‑1 (MQ=255)

                                                                             gCGA‑TAGTTTTGCCCGGGCCGT‑GTGCAGCCCCCATAGGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACTGTCGCCACCGAGCCGCAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  2:179940/238‑1 (MQ=255)

                                                                             gCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  1:830979/238‑1 (MQ=255)

                                                                             gCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  1:1048743/238‑1 (MQ=255)

                                                                             gCGA‑TAGTTTTACCCGTGCCGT‑GTTCCACCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  2:277449/238‑1 (MQ=255)

                                                                             gCGA‑GAGTTTTACCCGTGCCGT‑GTTCCTCCGCCATACGGCGCAGCGAAACTGACGCCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTCCG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  1:958708/238‑1 (MQ=255)

                                                                               ccgagagttttaccggcgcgtggtTCACCCGCCATCCGCCGCAGCGAAACTGACGGCAAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  2:792208/216‑1 (MQ=255)

                                                                                     ttttccccgtccgtt‑gtTCACCCGCCAAACGGCGCAGCAAAACTTACGGCTAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                            <  2:783451/225‑1 (MQ=255)

                                                                                      tttCTCCGTGCCGT‑TTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                            <  2:1377677/238‑1 (MQ=255)

                                                                                      tttATCCGTGCCGT‑TTTCAACCGCGATACGGCGCAGAGAAACTGACGGGGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                            <  1:385850/238‑1 (MQ=255)

                                                                                      tttATCCGTGCCGG‑TTTAACCCGCCATACGGCGCAGCGAACCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTgg                                                                                                                                            <  2:1101833/238‑1 (MQ=255)

                                                                                       ttgtCCGTGCCGT‑TTTCAGCCGTCATACGGCGCAGAGAAACTGACGGCGAGAAACGCCAAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCTTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                          <  2:1292695/236‑1 (MQ=255)

                                                                                       ttATCCGTGCAGT‑TTCCAACCGCCATCCGGCGCAGCGAAACGGACGGCGAGAAACGCCCAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                          <  1:644486/239‑1 (MQ=255)

                                                                                       ttATCCGCGCCTT‑GTCCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                          <  2:607770/239‑1 (MQ=255)

                                                                                       ttAGCCGGGCCGT‑GTTCACCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                          <  2:544950/239‑1 (MQ=255)

                                                                                       ttACCCGTGCCGT‑TTCCATCCGCCAACCGGCGCAGCGAAACTGACGGCGAGAACCGCACAAAACGGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                          <  2:1382619/239‑1 (MQ=255)

                                                                                       taagccgggccgt‑gTTCATCCGCCATACGGCGAAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGaa                                                                                                                                          <  1:579885/235‑1 (MQ=255)

                                                                                              tgccgt‑gTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGCGGGGTCCACTT‑‑GCCTTTTTTTCCGCCC‑CGCGCCC‑C‑GCCAAACACCACCCACCCTGGCCCCCACCTCCCCA‑‑CCAA‑ATTTTGGGTGC‑TTCGGTGCAAAACgaaaatagcggggaaaagcttt                                                                                                                                   >  2:1306597/1‑219 (MQ=255)

                                                                                              tgccgt‑gTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTTACTT‑CGCCCTTTTCACGCCCC‑GGCGCGC‑G‑GCCAAACAACACCGACACCGG‑GCCCCTCCGCCCAT‑ACCG‑CTTTGGGTTGC‑TACGGGGCCAAACgagagaattcgtggacaagcta                                                                                                                                   >  2:170369/1‑221 (MQ=255)

                                                                                               gccgt‑gTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑TCCCCTTTTTACGCCCC‑GGCCCGC‑G‑GCCAAAAATCACCCACCCTTG‑CACCATCTTCCCCT‑CCAG‑ATTTTGGTTGC‑TTCGGGGCAAATTCAGATAGTCGTGGGACAGC‑TTaa                                                                                                                                 >  2:929709/1‑239 (MQ=255)

                                                                                               gccgt‑gTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CCCCTTTTTTACGCCCC‑GGCGCGC‑G‑ACCAAACAACACCGACACCGG‑CACCATCTCCACAA‑CCAG‑ATTTGGGGTGC‑TACGGTGCAAATCGGGATAGTCGTGGAAAAGC‑TTaa                                                                                                                                 >  1:834845/1‑239 (MQ=255)

                                                                                               gccgt‑gTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CCCCTTTTTCACCCCCC‑CGCGCGC‑G‑CCCCAACACCACCCACCCCGG‑CACCATCTTCACCT‑CCCA‑CTTTGGGGTGC‑TACGGTGCCAATCGAGATAATCGTGGAAAAGC‑TTaa                                                                                                                                 >  2:1292722/1‑239 (MQ=255)

                                                                                                   t‑gtTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑Ggcgcg                                                                                                                                                                                                                                <  1:466285/147‑1 (MQ=255)

                                                                                                      ttCACCCGCCATACGGGGCAGCAAACCGGACGGCGAGAAACGCAAAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGAc                                                                                                                            <  2:428466/238‑1 (MQ=255)

                                                                                                         aTCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑TCCCCTTTTTACGCCCC‑CGCGCGC‑G‑ACCAAACAACCACCACACTGG‑CCCCCTCTGCCCAC‑CCAG‑ACTTTGGTTGG‑CACGGTGCAAATCGAGATAGTCGTGGAAACGC‑TTaaaataatct                                                                                                                         >  2:117224/1‑230 (MQ=255)

                                                                                                             gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCttt                                                                                                                    <  1:274719/239‑1 (MQ=255)

                                                                                                              ccATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CTCCTTTTTTACCCCCC‑CGCGCGC‑G‑Accccaca                                                                                                                                                                                                                    >  2:466285/1‑142 (MQ=255)

                                                                                                                aTACGGCGCAGCGAAACTGACGGCCAGAACCGCACAAACCTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                 <  1:1038953/239‑1 (MQ=255)

                                                                                                                aTACGGCCAAGCGAAACGGACGGAGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                 <  2:747739/239‑1 (MQ=255)

                                                                                                                  aCGGCGCAGGGAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCg                                                                                                               <  2:629551/239‑1 (MQ=255)

                                                                                                                  aCGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCg                                                                                                               <  2:1111227/239‑1 (MQ=255)

                                                                                                                   cGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑CGCGCGC‑G‑ACCACACATCACCCACCCCGG‑CACCACCTGCACATCCCAA‑ATTTGGTTTG‑‑TATCGGGCCAATCGAGAAAGTAGGCGAACCAC‑TTAATAAACTCTTCTTTTGGCgc                                                                                                              >  2:267617/1‑238 (MQ=255)

                                                                                                                   cGGCGCAGCGAAACGGACGGCGAGAAACGCAAAAAACGGCCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCgg                                                                                                                 <  2:775400/236‑1 (MQ=255)

                                                                                                                      cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGAt                                                                                                                                                    <  1:206756/199‑1 (MQ=255)

                                                                                                                      cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCgtgt                                                                                                            <  1:62231/238‑1 (MQ=255)

                                                                                                                       gcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACACTTCTTTCGGCGTGTaa                                                                                                          <  1:516048/239‑1 (MQ=255)

                                                                                                                        cAGCGAAACTGACGGCGAGAAACGCCCAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTCCG‑CCGTT‑AAGCATACGCAGCGGC‑GGTTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAAt                                                                                                         <  1:885248/239‑1 (MQ=255)

                                                                                                                         aGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCCCTT‑CCCCTTTTTCACCCCCC‑GGCGCAC‑C‑ACCAAAAAACACCGACCCCCG‑CGCCCTCTGCACCT‑CCAG‑ATTTTGGGTGC‑TACGGCGTAAAATGCAAAAGTAGTGGAACAACGTTAATAG‑CTTTTCtttttcgctggaatt                                                                                                        >  2:1069791/1‑227 (MQ=255)

                                                                                                                           cGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCCTTTTTACCCCCC‑GGCGCGC‑G‑GCCCAACAACACCGACCCTGG‑CACCATCCGCACAT‑CCCG‑ATTTTGGTTGC‑TACCGGGGAAATCGAGATAGTCGTTGACCAGC‑TTAATAGACTCTTCTTTCGGCGTGGAATCgg                                                                                                      >  2:40057/1‑239 (MQ=255)

                                                                                                                               acgaaCGGCGAGAAACGCACAAAACTGTCGGGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGGGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGaa                                                                                                  <  2:194048/235‑1 (MQ=255)

                                                                                                                                cTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTACGCCCC‑CGCGCGC‑G‑ACCAAACATCACCGACACTCG‑CCCCATCTGCACAC‑CCCG‑ATTTGGGTTGC‑TACGGTGCAAAACGAGATAGTCGTGGaa                                                                                                                                          >  2:206756/1‑199 (MQ=255)

                                                                                                                                                cacaAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTTACGCCCC‑GGCGCGC‑G‑ACCAAAAATCCACCAAACCGG‑AACCATCTCCCCCT‑TCCG‑ATTTTGGTTGG‑TAACGTGGAAAACGAGGTAGTCCTGGGAGCGA‑TTAATATAACTTTCTTTCTGCGGCTGATATATGGAAACAAGCGGCccgccgg                                                                                 >  2:103021/1‑238 (MQ=255)

                                                                                                                                                        tGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCttt                                                                                                                    <  1:1191882/196‑1 (MQ=255)

                                                                                                                                                             cgACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTCACGCCCC‑CGCGCGC‑G‑GCCAAACATCACCCACACCGG‑CACCATCTGCACCT‑CCCG‑ATTTGGGGTGC‑TACCGGGCAAATAGAGATAGTCGGGGGACAGC‑TTAATAAACTCTTCTTTCCGCGTGTAATAGGTGGACACACACGGGCCCCCGGGGATGAAcccaaa                                                                    >  2:452083/1‑237 (MQ=255)

                                                                                                                                                              gACCGAGCCACAGTACAGCAAAAAA‑TTTC‑TGTCCG‑CCGTT‑AAACATACGCAGCGGC‑GGTTTCCCTT‑CCCCTTTTTCCCCCCCC‑GGCGCGC‑G‑ACAAAACATCCCCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGGGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAg                                                                   <  1:378013/239‑1 (MQ=255)

                                                                                                                                                               gaccgagcaaaacaccagcaaaaa‑TTTC‑TTGCCGCCCTTT‑AACCAT‑CGCAGCGGG‑GGGTTTCCCT‑CCCCTTTTCCCCCCCCCGGGCGCGC‑A‑ACAAAACACCCCCGACACGGG‑CACCATCTGCACAT‑CCAG‑TTTTGGGTTGC‑TAGGGTAAAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAg                                                                   <  2:1365607/220‑1 (MQ=255)

                                                                                                                                                               aCCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCCACACTGG‑CACCCTCTGCACAT‑CCAG‑ATTTTGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTTATAGACTCTTCTTTCTGCGTGTAATAGGTGAAAACACGCGGCCCGCCGTGAATGAACAAATAg                                                                   >  1:210654/1‑238 (MQ=255)

                                                                                                                                                               aCCGAGCCACAGTAC‑GAGAAAAATTTTT‑TGGCCG‑CCGTT‑AACCATACGCAGCGGC‑GGGTCCACTT‑CCCTTTTTCCCCGCCCC‑GGCGCGA‑G‑TACAAACAACCCCACCGCTGA‑CCCCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTACAAATCGGGATAGTCGGGGAACAGA‑TTAATAACCTCTTCTTTCGGCGTTTAATAGGTGACCACCCGCGGCCCGCCGTGAATGAACACATAg                                                                   <  2:866010/238‑1 (MQ=255)

                                                                                                                                                                cggcccccaggtccggagAAAAA‑TTTC‑T‑TACC‑CCCTTAAAGCTTCCCCGCCGGG‑GGTTTCATTC‑CGCTTTTTCCCCGCCCC‑GGCCCGC‑G‑CCCAACAACCACCGACACTGC‑CACCATCTGCACAT‑CCAG‑ATTTGGTTTGC‑TACGGTCCAAATGGAGATAGGCGTGGAACAGC‑TAAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAgcg                                                                 <  2:1268869/222‑1 (MQ=255)

                                                                                                                                                                 cggcccccaagtccggcaaaaa‑TTTCTTGTCCG‑CCTTA‑AAGCAACCGCGGCGGC‑GGTTTCACTC‑CCCTTTTTCCCCGCCCG‑GGCGCCC‑G‑CCAAAACACCCCCGACACTGG‑CACCACCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAgcg                                                                 <  2:1195865/222‑1 (MQ=255)

                                                                                                                                                                               gCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGGTCACTT‑CGCCTTTTTTACGCCCC‑CGCGCGC‑G‑ACCCAACATCACCGACACCTG‑CACCATCTGCACAT‑CCAG‑ATTTTGGGTGC‑TACGGGGCAAATCGAGATAATCGTGGAACAGC‑TTAATAGACTCTTCTTTCTGCGTGTAATAGGGTAACAAACGCGGCCCGCCCGGTGGGAACACAAAGcgccgccagcgcgtgc                                                   >  2:1341860/1‑228 (MQ=255)

                                                                                                                                                                                                 cG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTTACGCCCC‑CGCGCGC‑G‑ACCCAACATCACCGACACCGG‑CCCCATCTGCCCAT‑CCCG‑ATTTGGGTTGC‑TACGGTGCAAATCCAGATAGTCGTGGGACAGC‑TTAATAGACTCTTCTTTCTGCGTGTAATAGGTGAACACACGCGGCCCGCCGCGAATTAAAACATAACGCGGCAGGAGCGGGCCGTCGATTTCCCGCgg                                   >  2:920172/1‑238 (MQ=255)

                                                                                                                                                                                                         taggcacgcgccgcGGC‑GCGTTCACTC‑CGCCTTTCCCACGCCCG‑GGCGCCC‑G‑ACAAAACATCACCGACACTGC‑CCCCATCTCACCAC‑CCGG‑ATTTGGTTTGC‑TACGGGGCAAATCGAGATGGTCGTGGAACAGC‑TAAAAAGACTCTCCTTTCGGCGTGTAATAGGGGAACACCCGCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTaa                             <  2:19322/230‑1 (MQ=255)

                                                                                                                                                                                                             ccacggaggcggc‑gGGTCGCCTT‑CTCC‑TTTGCCCGCCCC‑GGCGCGCGG‑ACCAAACACCCCCGACACTGG‑CACCATCTGCCCAT‑CCAG‑TTTTGGGTTGC‑TACGGGGCAAAGCGAGATAGTCGTGAAACGGC‑TAAATAGACTCTTCTTGCGGGGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                        <  2:916014/231‑1 (MQ=255)

                                                                                                                                                                                                                            tgTTCCCCT‑CCCCCTTTTCCCCCCCC‑GGGCGGC‑GCACCAAACCCCCCCGACACTGG‑CACCATCTGCACTC‑CCAG‑‑TTTGGGTTTCGTGCGGTGCAACTCGAGATAGTCGGGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGGGACCCCCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCAt            <  2:796402/237‑1 (MQ=255)

                                                                                                                                                                                                                                 ccctcccctttttcCACGCCCC‑GGCGCCC‑G‑ACAAAACACCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGAAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCa      <  1:920172/230‑1 (MQ=255)

                                                                                                                                                                                                                                 aCTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTaa                                                                                                          >  1:5304/1‑138 (MQ=255)

                                                                                                                                                                                                                                 aCTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTaa                                                                                                          <  2:5304/138‑1 (MQ=255)

                                                                                                                                                                                                                                     tcctcTTTTCCCCCCCCC‑GCCGCGC‑C‑ACAAAACCTCCCCCACACTGG‑CCCCCTCTGCACAT‑CCAG‑TTTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTGGGGGTGTAATAGGTGAACCCACGCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGaca  <  2:32343/235‑1 (MQ=255)

                                                                                                                                                                                                                                      cGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGac   >  1:180926/1‑237 (MQ=255)

                                                                                                                                                                                                                                       gCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGac   >  1:69688/1‑236 (MQ=255)

GCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGA‑CGGG‑CAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGA‑TAGTTTTATCCGTGCCGT‑GTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAA‑TTTC‑TGTACG‑CCGTT‑AAGCATACGCAGCGGC‑GGGTTCACTT‑CGCCTTTTTCACGCCCC‑GGCGCGC‑G‑ACCAAACATCACCGACACTGG‑CACCATCTGCACAT‑CCAG‑ATTTGGGTTGC‑TACGGTGCAAATCGAGATAGTCGTGGAACAGC‑TTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACA  >  NC_000913/4255831‑4256288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`