BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	375,168	G→T	4.9%	intergenic (+287/‑291)	mhpE → / → mhpT	4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,168	0	G	T	4.9%	344.6 / 5.2	117	intergenic (+287/‑291)	mhpE/mhpT	4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand): ref base G (51/56); new base T (4/2); total (57/60)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

Only 100 of 119 total aligned reads displayed.

CGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGC  >  NC_000913/374938‑375405

gggcgGGAACGCCTTATCCGGCCTACGGATGGCGTGAAAATTTGGAGGTCTGATAAGACGCGTTAGCGTCTCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGgcgtg                                                                                                                                                                                                                                           <  2:845235/237‑3 (MQ=255)

   cgTGAACGCCTTATCCGGCCTACGGAGGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCgaga                                                                                                                                                                                                                                        <  2:1085901/238‑1 (MQ=255)

            cTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCCGGGATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGGGAACGCCCTATCCGGGACACGGATGGCGTGGGAAttcttatgt                                                                                                                                                                                                                              >  2:1079896/1‑232 (MQ=11)

                    gCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCCGGCCTC‑TGCGCACGACTGCCGGAAGCGGCGTGAACGCCTCATCCGGCCTACGGGTTGCGCGGGAATTTGTAGGCCTggcaag                                                                                                                                                                                                                      >  2:725918/1‑234 (MQ=255)

                    gCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCCCGACTGCCGGATGCGGCGTGAACGGCTTAACCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAg                                                                                                                                                                                                                      >  2:279317/1‑239 (MQ=255)

                     ccTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAg                                                                                                                                                                                                                      >  1:184658/1‑238 (MQ=255)

                      cTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGa                                                                                                                                                                                                                     <  2:25542/238‑1 (MQ=255)

                              ggCGTGAGAATTTGTAGGTCTGATAAGTCGCGTTAGCGTCGCATCAGGCATCTGCGCGCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGc                                                                                                                                                                                                           <  1:1079896/239‑1 (MQ=255)

                              ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTCGCGTTGCATCAGGCATC‑TGCGCACGACCGCCGGATGCGGCGTGCACGCCTTATCCGGCCTCCGGGCGGCGGCGGAGATTGTAGCGCAGATAAGGAGCG‑TTAg                                                                                                                                                                                                            >  2:353089/1‑238 (MQ=11)

                              ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGGTGCGGCGTGAACGCCCTATCCGCCCTACGGTCTGCGCCGGCATTTGTAGGCCTGATAAGACTCG‑TTAGc                                                                                                                                                                                                           >  2:691107/1‑239 (MQ=11)

                              ggCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGc                                                                                                                                                                                                           >  1:736501/1‑239 (MQ=255)

                                cGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCg                                                                                                                                                                                                          <  2:1096014/238‑1 (MQ=40)

                                         ttGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCGCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAgg                                                                                                                                                                                                <  1:1070451/239‑1 (MQ=38)

                                         ttGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAgg                                                                                                                                                                                                <  2:184658/239‑1 (MQ=38)

                                             aGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt                                                                                                                                                                                             <  2:139786/238‑1 (MQ=38)

                                             aGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt                                                                                                                                                                                             <  1:481737/238‑1 (MQ=38)

                                             aGGACTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt                                                                                                                                                                                             <  1:73587/238‑1 (MQ=37)

                                                tCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑Tg                                                                                                                                                                                         >  2:87472/1‑238 (MQ=37)

                                                tCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGGCGCATCAGGCATC‑Tgc                                                                                                                                                                                        >  1:536228/1‑239 (MQ=37)

                                                  tGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAATACGCG‑TTACCGTCGCCTCAGGCATC‑Tgcg                                                                                                                                                                                       >  2:633452/1‑238 (MQ=19)

                                                    agaaGACGCGGTGGCGTGGCAGCAGGCAGCTGCGCACGGCTGCCGGATGCGGCGTGGACGCCTTGTGCGGCCTACGGAGGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCa                                                                                                                                                                                     <  2:497786/236‑1 (MQ=11)

                                                         aCGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACt                                                                                                                                                                                >  1:1108718/1‑238 (MQ=38)

                                                         aCGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACACTTCGCCCG‑TAAGCCGTAGGCCTGATAAGACGCGCAAAGCGTCGCATCAGGCAT‑‑GGCGCACGACTg                                                                                                                                                                               >  2:199757/1‑238 (MQ=11)

                                                              ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGACCGTCTTATCCGGCCCACGGGTGGCGCGGGCATTTGTAGGGCTGGTAAGACCCC‑TTAGCGGCCCATCAAGCCTC‑CGGGCAAGACTGCCggg                                                                                                                                                                          >  2:579494/1‑238 (MQ=11)

                                                              ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGa                                                                                                                                                                          >  1:1330350/1‑239 (MQ=38)

                                                              ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGa                                                                                                                                                                          >  1:981624/1‑239 (MQ=38)

                                                              ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGa                                                                                                                                                                          >  1:776030/1‑239 (MQ=38)

                                                              aaaGCGTCGCATCAGGCAT‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACACTTCGC‑CCGTAAGCCGTAGGCCTGATAAGACGCGCAAAGCGTCGCATCAGGCAT‑‑GGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCCGTCTACACTTCGC‑CCATAAACCGTAGGCCTGATAAACCGCGCATAGCCTCGCATCAGGCCGC‑TCCGCACAACttttctttg                                                                                                                                                                        >  2:484127/3‑231 (MQ=21)

                                                                aGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACACTTCGCCCG‑TAAGCCGTAGGCCTGATAAGACGCGCAAAGCGTCGCATCAGGCAT‑‑GGCGCACGACTGCCGGATgc                                                                                                                                                                       <  1:199757/239‑1 (MQ=11)

                                                                   gTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCC‑GATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATgcg                                                                                                                                                                      <  1:975449/237‑1 (MQ=38)

                                                                    tCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACGGCCCGACgcggc                                                                                                                                                                    >  2:317990/1‑239 (MQ=255)

                                                                    tCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAAACGCG‑GTAGCGCCCCATCCGGCATC‑TGCGCACGCCTGCCGGCTgcggc                                                                                                                                                                    >  2:862884/1‑239 (MQ=11)

                                                                    tCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCCGGCATC‑TGCGCACGACTGCCGGATgcggc                                                                                                                                                                    >  1:985744/1‑239 (MQ=39)

                                                                       cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTa                                                                                                                                                                 >  2:214131/1‑239 (MQ=40)

                                                                        aTCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTaa                                                                                                                                                                <  2:736501/239‑1 (MQ=255)

                                                                                 cTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCCCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCCTC‑TGCGCCCGACCGCCGGATGCGGGGTAAACCCCTTa                                                                                                                                                        >  2:1096597/1‑238 (MQ=255)

                                                                                  tGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                      <  2:568472/239‑1 (MQ=40)

                                                                                            tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCCCGGCTGCCGGATGCCGCGTGAAGGCCTTACCCGGCCCACGGGGGGCGCGAGACTTTTTCATCCAGATAAGACCCG‑CTAGCGTCGCATCAGGCATC‑TGCGCCAGACTGGCGGGTGCGCGGTAAGCGGCCTCTCCGGcacacgt                                                                                                                                            >  2:609101/1‑233 (MQ=11)

                                                                                             gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                                                                                                           <  1:841517/239‑1 (MQ=39)

                                                                                              ccGGGTGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                                                                                                           <  2:741017/238‑1 (MQ=39)

                                                                                              ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGTGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCCCGCCTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACGGAt                                                                                                                                          >  2:832798/1‑239 (MQ=255)

                                                                                              ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTCGGCCTGATCAGACGCG‑TTAGCGTCGCATCCGGCATC‑CTCGCACCACTGCCGCGTGCGGAGTAAACGCCCTATCCGGCCTCCgtgc                                                                                                                                          >  2:18694/1‑236 (MQ=11)

                                                                                                   tGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGgcg                                                                                                                                      <  2:776030/238‑1 (MQ=40)

                                                                                                        cgTGAGCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTATGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTAGCCGGCCTACGGGTGGCGGGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGGACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                                                                                                                 <  1:239800/238‑1 (MQ=255)

                                                                                                        cgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAgg                                                                                                                                                                                                <  2:13639/176‑1 (MQ=39)

                                                                                                        cgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAgg                                                                                                                                                                                                >  1:13639/1‑176 (MQ=39)

                                                                                                            aaCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGAc                                                                                                                                                                                                                    >  1:173622/1‑153 (MQ=25)

                                                                                                            aaCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCCGCCCACGGGGGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATA‑TGCGCACGACTGCCCGACGGGGCGTAACGCCCCTATCCGGCCCTCGGGCGGCGCGGGAATTTg                                                                                                                            >  2:354331/1‑239 (MQ=11)

                                                                                                                 cTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGc                                                                                                                       <  1:214131/239‑1 (MQ=255)

                                                                                                                    aTCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTg                                                                                                                    >  1:1011743/1‑239 (MQ=255)

                                                                                                                          ccTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAAGCCCTTATCCGGCCTACGGCTGGCGCGGGGATTTGTAGGCCTGCTAAg                                                                                                               >  2:506981/1‑238 (MQ=255)

                                                                                                                          ccTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCCACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCCTCAGGCATC‑TGCGCCCGCCTGCCGGGTGCTGCGTAAACGCCTTTTTCGGCCTACGGCTGGCGACGGACTTTGCAGGCCTGCTAAGa                                                                                                              >  2:523490/1‑239 (MQ=14)

                                                                                                                           cTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                                                                              <  2:1330350/238‑1 (MQ=255)

                                                                                                                           cTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGa                                                                                                              <  1:279317/238‑1 (MQ=255)

                                                                                                                                   ggCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGc                                                                                                     >  1:164176/1‑239 (MQ=255)

                                                                                                                                    gcgcGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCg                                                                                                    >  1:297927/1‑239 (MQ=255)

                                                                                                                                    gcgcGGGAATTTGTAGGCCTGATAAGAAGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACCCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACCGCCGGATGCGGCGCAAACGCCTCATCCGGCCTACGGATTGCGCGGGCATTTGTATGCTCGATaaagacaggtagcg                                                                                                    >  2:687712/1‑227 (MQ=14)

                                                                                                                                      gcgGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                   <  2:951779/238‑1 (MQ=40)

                                                                                                                                           aaTTTGTAGGCCTGAGAAGAGGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCAt                                                                                              <  2:446217/238‑1 (MQ=255)

                                                                                                                                            aTTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCa                                                                                            <  1:89345/239‑1 (MQ=38)

                                                                                                                                                  aGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt                                                                                       <  1:87472/238‑1 (MQ=38)

                                                                                                                                                     ccTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCAt                                                                                                                                                                                             <  1:576852/134‑1 (MQ=35)

                                                                                                                                                      cTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGca                                                                                 <  2:600173/239‑1 (MQ=37)

                                                                                                                                                         aTAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACCCCTTATCCGGCCTACGGATGGCGCGGGGATTTGTAGGCCTGATAAGAAGCGGTGGCGGCGCATCCGGCATC‑TGccca                                                                               >  2:221798/1‑238 (MQ=11)

                                                                                                                                                           aaGACGCG‑TTAGCGTCGCATCAGGCGTC‑TGCGCCCGAATGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGGATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTGTCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGa                                                                            <  1:1321985/239‑1 (MQ=255)

                                                                                                                                                              aCGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAAGCGTTAGCGGCGCATCAGGCATC‑TGCACACGAcc                                                                          >  2:248723/1‑237 (MQ=255)

                                                                                                                                                              aCGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGGCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTAAGGATGGCGCGGGCATTTGTTCGCCTGATAAGACGCGTTCGCGTTCCACCAGGCATC‑TGCACACGACt                                                                          >  2:1302018/1‑238 (MQ=11)

                                                                                                                                                                    ttAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGGATTTGTGGGCCTGATAAGACGCGTTAGCGTCGCCTCAGGCGTC‑TGCCCAAGAGTGCCGGa                                                                    >  2:200620/1‑239 (MQ=255)

                                                                                                                                                                    ttAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGa                                                                    >  1:1210915/1‑239 (MQ=38)

                                                                                                                                                                    ccaGCGTCGCATCAGGCATCATGCAC‑CAATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGTT‑‑CG‑GACTATTTGTAGGCACGATAAGACGCG‑ACAGCGTCGCATCAGGCATCATGCAC‑CAATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGTT‑‑CG‑GACTATTTGTAGGCACGATAAGACGCGACAGCGTCGCATCAGGCATCATGCAC‑CAATTGCCGGATGCggc                                                              >  2:902169/3‑237 (MQ=17)

                                                                                                                                                                     tAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGa                                                                    <  2:1011743/238‑1 (MQ=38)

                                                                                                                                                                      aGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGAt                                                                   >  1:405362/1‑238 (MQ=38)

                                                                                                                                                                             cATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATaa                                                            >  1:353616/1‑238 (MQ=40)

                                                                                                                                                                               tCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGGCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACg                                                         <  1:353089/239‑1 (MQ=255)

                                                                                                                                                                                     aTC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                   <  1:68191/239‑1 (MQ=255)

                                                                                                                                                                                     aTC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                   <  1:806881/239‑1 (MQ=255)

                                                                                                                                                                                      tC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGt                                                   >  1:817300/1‑238 (MQ=255)

                                                                                                                                                                                           cgcACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCgg                                               >  2:127537/1‑238 (MQ=255)

                                                                                                                                                                                                gACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACa                                         >  1:1145363/1‑239 (MQ=255)

                                                                                                                                                                                                  cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt                                                                                                                                                                                                         <  1:629165/79‑1 (MQ=39)

                                                                                                                                                                                                  cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGt                                                                                                                                                                                                         >  2:629165/1‑79 (MQ=39)

                                                                                                                                                                                                  cTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAATTGTAAGCCTGATAAGACGCGTTAGCGTCGCGTCAGGCCAC‑GGCACACGACCGGCGGATGCGCTAAACGTCtttgtctggctacagt                                       >  2:500113/1‑225 (MQ=14)

                                                                                                                                                                                                   tGCCGGATGCGGTGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                      <  1:832798/239‑1 (MQ=255)

                                                                                                                                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt                                      <  2:405362/239‑1 (MQ=255)

                                                                                                                                                                                                            cggcgTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGCTAAACGTCTTGTCCGGCCTACATTTCGCCCGTAg                             >  2:950035/1‑239 (MQ=255)

                                                                                                                                                                                                               cgTGACCGCCTTATCCGGCGTACGGGGGGCGGGAGAATTTGTAGGCCTGATAAGACGCG‑GTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                           <  2:1132948/238‑1 (MQ=255)

                                                                                                                                                                                                               cgTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                           <  2:981624/238‑1 (MQ=255)

                                                                                                                                                                                                               cgTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                           <  2:1210915/238‑1 (MQ=255)

                                                                                                                                                                                                               cgTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc                           <  1:648440/238‑1 (MQ=255)

                                                                                                                                                                                                                 tGAACGCCGTGTCCGGGCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAg                         <  1:725918/238‑1 (MQ=255)

                                                                                                                                                                                                                   aaCGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCa                      >  2:1388302/1‑239 (MQ=255)

                                                                                                                                                                                                                        cTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa                 <  2:164176/239‑1 (MQ=255)

                                                                                                                                                                                                                        cTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaa                  <  1:886922/238‑1 (MQ=255)

                                                                                                                                                                                                                           aTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGgcg                                                                                                                                      >  2:1114747/1‑120 (MQ=255)

                                                                                                                                                                                                                           aTCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGgcg                                                                                                                                      <  1:1114747/120‑1 (MQ=255)

                                                                                                                                                                                                                            tCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCa                                                                                            <  1:194486/161‑1 (MQ=38)

                                                                                                                                                                                                                                gCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGCCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTg         >  1:1349864/1‑239 (MQ=255)

                                                                                                                                                                                                                                     cGGCTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCAGGCCTCCATTTCGCCCGTCGGCACTGATTAACTCGTTCTGATAAc    >  2:843225/1‑239 (MQ=17)

                                                                                                                                                                                                                                      gggTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGca                                                                                 >  2:194486/1‑161 (MQ=39)

                                                                                                                                                                                                                                        gTGGCGCGAGAATTTGTAGGACTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCGTAGCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTAcgc  <  2:517876/238‑1 (MQ=255)

CGGCGTGAACGCCTTATCCGGCCTACGGATGGCGTGAGAATTTGTAGGTCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCG‑TTAGCGTCGCATCAGGCATC‑TGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATC‑TGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGC  >  NC_000913/374938‑375405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`