Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,751 A→G 9.1% E611E (GAA→GAG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,7510AG 9.1% 159.4 / 3.3 69E611E (GAA→GAGrsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (40/22);  new base G (2/5);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

AGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCT  >  NC_000913/1708514‑1708988
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
aGCGCAAGCCAAGGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAc                                                                                                                                                                                                                                              <  2:624690/239‑1 (MQ=255)
    cAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCACAGCGCGCCAGCTGGAACAGCAACAGGCTAACTCGGAACCaga                                                                                                                                                                                                                                          >  1:761583/1‑239 (MQ=255)
    cAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCaga                                                                                                                                                                                                                                          >  1:322333/1‑239 (MQ=255)
      aGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCAGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCcgaaa                                                                                                                                                                                                                                        >  2:121391/1‑238 (MQ=255)
         aaacgCGAAACAGGCAGAACGGCAGCAAAATAACGACGCAGAACCCGTGGCTGATCCACGAAAAACGGCCGTTGAAGCAGCTATTGCCCGCGCAAAAGCGCGCAAGCTGGAACAGCAACAGGCTACTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaaga                                                                                                                                                                                                                                       <  2:1052309/233‑1 (MQ=18)
            agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAGCCAAAGCACTCAAACCGGACCAGCAACAGGCTAATGAGGAGCCCGAAATACAgg                                                                                                                                                                                                                                  >  2:654488/1‑239 (MQ=18)
            agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGCACCAGAAGAACAgg                                                                                                                                                                                                                                  >  1:435428/1‑239 (MQ=255)
            agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                                                                                                                                  >  1:215156/1‑239 (MQ=255)
                 aagcggCAGAACCGCATCAAACTAACGACGCCGCACCCGTTGCTAACCCACGAAAAACTGCCGTAGGGGCAGCTCGCGCCCCCCCCAAGGCGGGGAAACTGGAACAGCAGCAGGCTAATGCGGACCCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                                                                                                                             <  2:63313/235‑1 (MQ=17)
                        aGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCa                                                                                                                                                                                                                      >  2:1136977/1‑239 (MQ=255)
                        aGAACGGCAGGAAACTAACGCGCGAGAACCCGCGGATAACCGACGAAAAACGGCCGATGAAGCAGCTAGCGAAGGGACCAAAGGAGGCAAACTGGAACAGCAACAGGCTAATGCGGACCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCa                                                                                                                                                                                                                      <  2:1007720/239‑1 (MQ=14)
                              gcgacaAACCAACGCCGCCGCAACCGGTGCGGAACCAATAAAAACTGCCGTTGAAGAAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg                                                                                                                                                                                                                <  2:1310804/235‑1 (MQ=17)
                                   aaaCTAACGACGCAGGACCCGGTGCTGAACCAGGAAAACCTGCCGTTGAAGCAGCGATCGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAgctgcc                                                                                                                                                                                                              <  2:162805/236‑1 (MQ=14)
                                       tAACGACGCCGCAACCGTTGACAACCACCCAAAAACGGCCGTGAAAGCAGCCATCGACCGCACCAAAGCGCGCAAGCTGGAACAGCAGCAGGCAAATGCAGAACCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                       <  2:698934/239‑1 (MQ=14)
                                        aaacgcccaagcaCCCGTGGCGGATCCGCGGAAAACGGCCGTTGAAGCAGCTAGCCCCCGCGCAAAAGCGCGCAAGCGGGAACAGCAACAGGCAAATGCGGAACCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                       <  2:215156/230‑1 (MQ=14)
                                        aaCGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGc                                                                                                                                                                                                      <  1:149063/239‑1 (MQ=255)
                                                  gcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCAGAACCAGAAGAACAGGTCGGTCCGTTAAAAGCCTCCGGCGAAGCCGccgatg                                                                                                                                                                                              >  2:824818/1‑233 (MQ=17)
                                                  accaagcgggatGACCCACGAAAACCGGCAGGCGAAGAAGCTAGTGCCCGCGAAAACGCGCCCAAGCTGGAACAACAACAGGCAAATGCGGACCCAGAACAACAGGACGAACCGCGAAAAGCGGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                                                                             <  2:822405/228‑1 (MQ=11)
                                                   caagcggggataacacACGAAAAACTGCCGTGGAAGCAGCTATCACCCGCGACAAAGCGCGCAAGCTGGAACAGCAACAGGATAATGCGGACCCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                                                                             <  2:996296/223‑1 (MQ=14)
                                                           gCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGAAACAGGCTAATGCGGAACCAGACGAACAGGTCGATCCGCGCAAAGACGCCGTCGAAGCCGCTATTGCCCGTGCCaa                                                                                                                                                                                    >  1:492788/1‑239 (MQ=255)
                                                                tCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCTCCGTCGACGCCGCAATTGCCCGAGCCCAAGCa                                                                                                                                                                                >  2:661031/1‑238 (MQ=14)
                                                                 ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                              <  1:1192316/239‑1 (MQ=255)
                                                                 ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGATGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGcgc                                                                                                                                                                               >  1:1328852/1‑236 (MQ=255)
                                                                    cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAGGCACGCaa                                                                                                                                                                           >  1:339687/1‑239 (MQ=255)
                                                                           cTGCCGTGGAGGCCGCTATCGCCCCCGCCAAAGCGCGAAAGCTGGAACAGCACCTGGGTAAGGCGGACCAAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                    <  2:263114/239‑1 (MQ=14)
                                                                           cTGCCGCTGAAGAGGCTATCGCCCGCCCCAAAGCGCGCAAGCTGGAACAGCAGCAGCCAAATGCGGACCCAGCACACCAGGCCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                    <  2:302878/239‑1 (MQ=17)
                                                                              ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCCACGCCGCCGTCGCCGCCGCTATTGCCCGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGGACCAGACCAACGGGTCGCTCCGCGCAAAACGGCAGGCGCCGCGGCTCTTGCCCGCGCTCAAGCCCgacaacccgacca                                                                                                                                                                  >  2:337997/1‑226 (MQ=11)
                                                                              ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa                                                                                                                                                                  >  1:263114/1‑238 (MQ=255)
                                                                                tctagagcccaactgccccGCGCAAAGCCCAGAAGCCTGAACCGGAAGGGGCGAATGCGGCACCAGAACACCAGGTCGACCCGCGCAACGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                              <  2:43356/223‑1 (MQ=14)
                                                                                 aagaaccagcccatcaccccccaaaaGCGGCCAAGCAGGAACAGCAACAGCCAAATGCGGACGCAGCACCCGAGGCCGAACCGCGTAAAACCGCCGTCGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                              <  2:322333/216‑1 (MQ=11)
                                                                                  tGAAGGAGCTAGCGCCCGCGCCAAAACGGGGAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                              <  2:1100185/238‑1 (MQ=255)
                                                                                  tGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                              <  1:1136977/238‑1 (MQ=255)
                                                                                        agcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATACGCGCAACGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCCCTCAAACCAGCACAGCAACCGGCTa                                                                                                                                                       >  2:1335130/1‑239 (MQ=11)
                                                                                                  cgcgCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCCAAGCACGCAAACCGGCACAACAACAGGCTAAAGCGGAGca                                                                                                                                             >  2:1283137/1‑238 (MQ=11)
                                                                                                    cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCCCCTGCCATGGCCCGGGCACAGGCACGCAACCTGGAACACCACCAGCCTAATCCCggacaag                                                                                                                                           >  2:1093391/1‑233 (MQ=14)
                                                                                                    cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACCGGCAAATCACGAGCCac                                                                                                                                           >  2:144466/1‑238 (MQ=255)
                                                                                                                     tggacgaatcacagtccacgggggAACCAGAAAAACAGGCCACGCCGCGCAAGACCGCCGTCGAAGCCGCTCTTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                                                          <  2:1356229/217‑1 (MQ=14)
                                                                                                                             cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCCACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGAACTACAGGTCGTTCCGCGCAAAg                                                                                                                   >  1:337808/1‑238 (MQ=255)
                                                                                                                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGACGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGTGCCAAACCACGCAAGCTGGAACAGCAACAGGCTCATGCGCAGCTACAACAACAGCGCGACCCGCGCAAAGCCGCCGTCGAATCCGCt                                                                                                  >  2:1089432/1‑239 (MQ=17)
                                                                                                                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGTGCCAGAACAACAGGTCGATCCGCCCAAAGCCGCAGTCGCAGCGGca                                                                                                  >  2:120433/1‑238 (MQ=14)
                                                                                                                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGGGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGCCGAAGCCGCt                                                                                                  >  1:43356/1‑239 (MQ=255)
                                                                                                                                             aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACGACAGGTCGCTCCGCGCACAGCCGCCGTCGAagcagcc                                                                                                  >  2:264038/1‑238 (MQ=255)
                                                                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCCACAGGCTAATGCCGAGCCACAAGAACAGGTCGCTCCGCGCAAAGCCGCCGTCGCAGCGCCCATTGCCCGAGCCa                                                                                     >  2:324662/1‑239 (MQ=14)
                                                                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                              >  1:923384/1‑166 (MQ=255)
                                                                                                                                                          ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                              <  2:923384/166‑1 (MQ=255)
                                                                                                                                                           gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCCCAAAGCCGCCGACGAAGCCGCCATTGcccacgccca                                                                                    >  2:941489/1‑232 (MQ=255)
                                                                                                                                                             cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCaaa                                                                                   >  1:1210759/1‑238 (MQ=255)
                                                                                                                                                             cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAACAACAGGTCGATCCGCGCAAATCCGCCGTTGCCGCCGCCATAGCCCGAGcacaa                                                                                   >  2:1006311/1‑234 (MQ=14)
                                                                                                                                                                tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCCGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAACCCGCTCTTGCCCGAGCCCAAgcg                                                                                >  2:1292309/1‑238 (MQ=255)
                                                                                                                                                                     gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCTGCTATTGCCCGCGCCAAAGCGCGCaa                                                                           >  1:426255/1‑238 (MQ=255)
                                                                                                                                                                                   gAAGCCGCTATTCCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAGCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAcc                                                            <  1:1198251/239‑1 (MQ=255)
                                                                                                                                                                                       ccGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                              <  2:1198296/121‑1 (MQ=255)
                                                                                                                                                                                           tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                            <  2:68211/151‑1 (MQ=255)
                                                                                                                                                                                           tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                            >  1:68211/1‑151 (MQ=255)
                                                                                                                                                                                                 ccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                    >  1:1198296/1‑121 (MQ=38)
                                                                                                                                                                                                   gggCCAAAGCGGCCACGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCa                                            <  1:824818/237‑1 (MQ=255)
                                                                                                                                                                                                    tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCGCGAGCCAAAGCGCGCAAACCGGAACAGCACCAAGCTAATGCTGAGCCag                                           >  2:825426/1‑239 (MQ=11)
                                                                                                                                                                                                    tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGCCGGAGCCGCACTAGGGCGTGCCAAAGCACGCCAGCGGGAACAGCAAACGGCTAATGCAGacactg                                           >  2:852679/1‑234 (MQ=11)
                                                                                                                                                                                                    tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCGACTATTTCCCGAGCCCAAGCGCGCAAACCGGAACAGCAACCGGCTAATACGGAGCCag                                           >  2:192851/1‑239 (MQ=255)
                                                                                                                                                                                                        aacacggggaaacgggAAAAGCAGCAGGATAATAACGAGGCAGAAAACCAGGATGACACGCGAAAAACCGCCGTCGAAGCCGCTATTGCCGGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                       <  2:349875/225‑1 (MQ=14)
                                                                                                                                                                                                             gcgcAAGCGGGAACAGCAACAGGATAATGCGGAACCAGAAGACCAGGTCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAgg                                  <  1:752552/239‑1 (MQ=255)
                                                                                                                                                                                                              cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAGGCCCGCAAACGGGAGCAGCACCAGGCTATCGCGGAGCCAGAAGAACGGGt                                 >  2:949724/1‑239 (MQ=14)
                                                                                                                                                                                                                         aCAGCAACAGGCTAATGCAGAACCAGAAACACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACAGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCa                      <  1:260181/239‑1 (MQ=255)
                                                                                                                                                                                                                             cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGCACAGCAACCGGCTAATGCGGAGCCAGAAGCACAGGTTGAACCGCGCaaaa                   >  2:289476/1‑237 (MQ=255)
                                                                                                                                                                                                                             cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACCGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCgcgccacg                   >  2:511790/1‑234 (MQ=255)
                                                                                                                                                                                                                             cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTCATGCGGAGCCCGAACAGCAGGCTGATACGCGCaaac                   >  2:712440/1‑237 (MQ=255)
                                                                                                                                                                                                                             cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAg                   >  1:426027/1‑238 (MQ=255)
                                                                                                                                                                                                                             cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCGGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGGGCAAACCGGAACAGCAACAGGCTAATGAGGAGCCAGAAAAACAGGTCGATCCCCCCCAAGc                  >  2:580034/1‑239 (MQ=11)
                                                                                                                                                                                                                                        tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGaaa       >  1:605865/1‑238 (MQ=255)
                                                                                                                                                                                                                                             aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAGGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCggac  >  2:603181/1‑237 (MQ=255)
                                                                                                                                                                                                                                             aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGGCGAAGCCGCTATTGCCCGCGCCAGAGCTCGGAAGCTGGCACAGCAACCGGCAACGGCGGAGCCAGAAGAACAGGTTAGTCTACGCCAAGCtccgggtcccgcgccg  >  2:137220/1‑224 (MQ=14)
                                                                                                                                                                                                                                             aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCCGCCGTCGCAGCGGca  >  2:721750/1‑238 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
AGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCT  >  NC_000913/1708514‑1708988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: