Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,805 T→C 16.3% R629R (CGT→CGC rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,8050TC16.3% 120.4 / 3.9 66R629R (CGT→CGCrsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (29/18);  new base C (10/2);  total (44/22)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACC  >  NC_000913/1708572‑1709041
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
gCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGAAACAGGCTAATGCGGAACCAGACGAACAGGTCGATCCGCGCAAAGACGCCGTCGAAGCCGCTATTGCCCGTGCCaa                                                                                                                                                                                                                                         >  1:492788/1‑239 (MQ=255)
     tCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCTCCGTCGACGCCGCAATTGCCCGAGCCCAAGCa                                                                                                                                                                                                                                     >  2:661031/1‑238 (MQ=14)
      ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                                                                   <  1:1192316/239‑1 (MQ=255)
      ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGATGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGcgc                                                                                                                                                                                                                                    >  1:1328852/1‑236 (MQ=255)
         cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAGGCACGCaa                                                                                                                                                                                                                                >  1:339687/1‑239 (MQ=255)
                cTGCCGTGGAGGCCGCTATCGCCCCCGCCAAAGCGCGAAAGCTGGAACAGCACCTGGGTAAGGCGGACCAAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                                                                         <  2:263114/239‑1 (MQ=14)
                cTGCCGCTGAAGAGGCTATCGCCCGCCCCAAAGCGCGCAAGCTGGAACAGCAGCAGCCAAATGCGGACCCAGCACACCAGGCCGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                                                                         <  2:302878/239‑1 (MQ=17)
                   ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCCACGCCGCCGTCGCCGCCGCTATTGCCCGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGGACCAGACCAACGGGTCGCTCCGCGCAAAACGGCAGGCGCCGCGGCTCTTGCCCGCGCTCAAGCCCgacaacccgacca                                                                                                                                                                                                                       >  2:337997/1‑226 (MQ=11)
                   ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACa                                                                                                                                                                                                                       >  1:263114/1‑238 (MQ=255)
                     tctagagcccaactgccccGCGCAAAGCCCAGAAGCCTGAACCGGAAGGGGCGAATGCGGCACCAGAACACCAGGTCGACCCGCGCAACGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                   <  2:43356/223‑1 (MQ=14)
                      aagaaccagcccatcaccccccaaaaGCGGCCAAGCAGGAACAGCAACAGCCAAATGCGGACGCAGCACCCGAGGCCGAACCGCGTAAAACCGCCGTCGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                   <  2:322333/216‑1 (MQ=11)
                       tGAAGGAGCTAGCGCCCGCGCCAAAACGGGGAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                   <  2:1100185/238‑1 (MQ=255)
                       tGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                   <  1:1136977/238‑1 (MQ=255)
                             agcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATACGCGCAACGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCCCTCAAACCAGCACAGCAACCGGCTa                                                                                                                                                                                                            >  2:1335130/1‑239 (MQ=11)
                                       cgcgCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCCAAGCACGCAAACCGGCACAACAACAGGCTAAAGCGGAGca                                                                                                                                                                                                  >  2:1283137/1‑238 (MQ=11)
                                         cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCCCCTGCCATGGCCCGGGCACAGGCACGCAACCTGGAACACCACCAGCCTAATCCCggacaag                                                                                                                                                                                                >  2:1093391/1‑233 (MQ=14)
                                         cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACCGGCAAATCACGAGCCac                                                                                                                                                                                                >  2:144466/1‑238 (MQ=255)
                                                          tggacgaatcacagtccacgggggAACCAGAAAAACAGGCCACGCCGCGCAAGACCGCCGTCGAAGCCGCTCTTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcg                                                                                                                                                                               <  2:1356229/217‑1 (MQ=14)
                                                                  cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCCACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGAACTACAGGTCGTTCCGCGCAAAg                                                                                                                                                                        >  1:337808/1‑238 (MQ=255)
                                                                                  aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGACGAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGTGCCAAACCACGCAAGCTGGAACAGCAACAGGCTCATGCGCAGCTACAACAACAGCGCGACCCGCGCAAAGCCGCCGTCGAATCCGCt                                                                                                                                                       >  2:1089432/1‑239 (MQ=17)
                                                                                  aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGTGCCAGAACAACAGGTCGATCCGCCCAAAGCCGCAGTCGCAGCGGca                                                                                                                                                       >  2:120433/1‑238 (MQ=14)
                                                                                  aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGGGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGCCGAAGCCGCt                                                                                                                                                       >  1:43356/1‑239 (MQ=255)
                                                                                  aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACGACAGGTCGCTCCGCGCACAGCCGCCGTCGAagcagcc                                                                                                                                                       >  2:264038/1‑238 (MQ=255)
                                                                                               ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCCACAGGCTAATGCCGAGCCACAAGAACAGGTCGCTCCGCGCAAAGCCGCCGTCGCAGCGCCCATTGCCCGAGCCa                                                                                                                                          >  2:324662/1‑239 (MQ=14)
                                                                                               ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                   >  1:923384/1‑166 (MQ=255)
                                                                                               ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                   <  2:923384/166‑1 (MQ=255)
                                                                                                gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCCCAAAGCCGCCGACGAAGCCGCCATTGcccacgccca                                                                                                                                         >  2:941489/1‑232 (MQ=255)
                                                                                                  cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCaaa                                                                                                                                        >  1:1210759/1‑238 (MQ=255)
                                                                                                  cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAACAACAGGTCGATCCGCGCAAATCCGCCGTTGCCGCCGCCATAGCCCGAGcacaa                                                                                                                                        >  2:1006311/1‑234 (MQ=14)
                                                                                                     tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCCGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAACCCGCTCTTGCCCGAGCCCAAgcg                                                                                                                                     >  2:1292309/1‑238 (MQ=255)
                                                                                                          gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCTGCTATTGCCCGCGCCAAAGCGCGCaa                                                                                                                                >  1:426255/1‑238 (MQ=255)
                                                                                                                        gAAGCCGCTATTCCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAAGCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAcc                                                                                                                 <  1:1198251/239‑1 (MQ=255)
                                                                                                                            ccGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACg                                                                                                                                                                                                                                   <  2:1198296/121‑1 (MQ=255)
                                                                                                                                tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                                                                                 <  2:68211/151‑1 (MQ=255)
                                                                                                                                tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                                                                                 >  1:68211/1‑151 (MQ=255)
                                                                                                                                      ccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa                                                                                                                                                                                                                         >  1:1198296/1‑121 (MQ=38)
                                                                                                                                        gggCCAAAGCGGCCACGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCa                                                                                                 <  1:824818/237‑1 (MQ=255)
                                                                                                                                         tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCGCGAGCCAAAGCGCGCAAACCGGAACAGCACCAAGCTAATGCTGAGCCag                                                                                                >  2:825426/1‑239 (MQ=11)
                                                                                                                                         tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGCCGGAGCCGCACTAGGGCGTGCCAAAGCACGCCAGCGGGAACAGCAAACGGCTAATGCAGacactg                                                                                                >  2:852679/1‑234 (MQ=11)
                                                                                                                                         tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCGACTATTTCCCGAGCCCAAGCGCGCAAACCGGAACAGCAACCGGCTAATACGGAGCCag                                                                                                >  2:192851/1‑239 (MQ=255)
                                                                                                                                             aacacggggaaacgggAAAAGCAGCAGGATAATAACGAGGCAGAAAACCAGGATGACACGCGAAAAACCGCCGTCGAAGCCGCTATTGCCGGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                            <  2:349875/225‑1 (MQ=14)
                                                                                                                                                  gcgcAAGCGGGAACAGCAACAGGATAATGCGGAACCAGAAGACCAGGTCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAgg                                                                                       <  1:752552/239‑1 (MQ=255)
                                                                                                                                                   cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAGGCCCGCAAACGGGAGCAGCACCAGGCTATCGCGGAGCCAGAAGAACGGGt                                                                                      >  2:949724/1‑239 (MQ=14)
                                                                                                                                                              aCAGCAACAGGCTAATGCAGAACCAGAAACACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACAGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCa                                                                           <  1:260181/239‑1 (MQ=255)
                                                                                                                                                                  cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGCACAGCAACCGGCTAATGCGGAGCCAGAAGCACAGGTTGAACCGCGCaaaa                                                                        >  2:289476/1‑237 (MQ=255)
                                                                                                                                                                  cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACCGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCgcgccacg                                                                        >  2:511790/1‑234 (MQ=255)
                                                                                                                                                                  cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTCATGCGGAGCCCGAACAGCAGGCTGATACGCGCaaac                                                                        >  2:712440/1‑237 (MQ=255)
                                                                                                                                                                  cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAg                                                                        >  1:426027/1‑238 (MQ=255)
                                                                                                                                                                  cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCGGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGGGCAAACCGGAACAGCAACAGGCTAATGAGGAGCCAGAAAAACAGGTCGATCCCCCCCAAGc                                                                       >  2:580034/1‑239 (MQ=11)
                                                                                                                                                                             tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGaaa                                                            >  1:605865/1‑238 (MQ=255)
                                                                                                                                                                                  aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAGGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCggac                                                       >  2:603181/1‑237 (MQ=255)
                                                                                                                                                                                  aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGGCGAAGCCGCTATTGCCCGCGCCAGAGCTCGGAAGCTGGCACAGCAACCGGCAACGGCGGAGCCAGAAGAACAGGTTAGTCTACGCCAAGCtccgggtcccgcgccg                                                       >  2:137220/1‑224 (MQ=14)
                                                                                                                                                                                  aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCCGCCGTCGCAGCGGca                                                       >  2:721750/1‑238 (MQ=255)
                                                                                                                                                                                        aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCTCTATTGGCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa                                                 <  1:264038/239‑1 (MQ=255)
                                                                                                                                                                                        aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGc                                                  >  2:555539/1‑238 (MQ=255)
                                                                                                                                                                                         agacccggTCGACCGGCGCAAAGCGTCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGTGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa                                                 <  2:1328852/232‑1 (MQ=14)
                                                                                                                                                                                         agaaCAGGGCGAACCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa                                                 <  1:252700/238‑1 (MQ=255)
                                                                                                                                                                                                  tgATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGAGGAGCCCGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGACGCGGCCATTGCACGGGCCaac                                        >  2:352144/2‑237 (MQ=255)
                                                                                                                                                                                                  cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAGGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCaag                                        >  1:441876/1‑237 (MQ=255)
                                                                                                                                                                                                     tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGCTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCAc                                    >  1:18388/1‑239 (MQ=255)
                                                                                                                                                                                                      ccGCGCAAAGCGGCCGTTGAAGCCGCGAGTGCCCGGGCCAAAGCACGCAAGCGGGGACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGCCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                                   <  2:435428/239‑1 (MQ=18)
                                                                                                                                                                                                      ccGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAGAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                                   <  1:144466/239‑1 (MQ=255)
                                                                                                                                                                                                          gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTTGCAGCGGCCAATACACGCGCCACAACACGCaa                                >  2:177391/1‑238 (MQ=17)
                                                                                                                                                                                                          gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCaa                                >  1:436618/1‑238 (MQ=255)
                                                                                                                                                                                                                 cgccgTCGAAGCCGCTATTGGCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAAc                        <  1:1089432/239‑1 (MQ=255)
                                                                                                                                                                                                                                       cGTGCCAAAGCAAGCAAGCTGGAACAGCAACAGGCTACTGCCGAGCCAGACCAACAGGTCGATCCGCGCAAAGCCGACGTCGAAGCCGCTATTGCCCGAGCTAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGGCAGAAGAACAGGTTGATTTGCGCAATGCCGCTGCCGACGCGGTCATTGTACGCGCTATAGCCAGAAAGCTGGCACCGCTACACGCTAATGCGGttac  >  2:1254356/1‑236 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            
GCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACC  >  NC_000913/1708572‑1709041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: