Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,040,129 | A→C | 21.3% | T218P (ACA→CCA) | yedY → | membrane‑anchored, periplasmic TMAO, DMSO reductase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,040,129 | 0 | A | C | 21.3% | 109.9 / 3.8 | 59 | T218P (ACA→CCA) | yedY | membrane‑anchored, periplasmic TMAO, DMSO reductase |
Reads supporting (aligned to +/- strand): ref base A (14/30); new base C (7/7); total (22/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.38e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAA > NC_000913/2039894‑2040362 | gCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTcaca < 1:93252/238‑1 (MQ=255) cGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCGTTATCGGCGGTGGGCGGAAAAATCCTTTTGTCGAAGGGGTGTGTTTCGGCGGAGGAATAGATCCGCTCacacc > 1:514380/1‑238 (MQ=255) atgtaacGTTTGCGCCGCGCGGCAGAGTGGGCGGTTGTGGTGCCGTGGATTGTTCATGCGGTGCACAAATTGCGGGCGCTTGCCGAACC‑TACGAGCAATGCGAAGTATGGCGCTTCCGAAACAA‑GTTAGGCACCGGAA‑CA‑GATGCCGGGCCAGCAGGACCGCTTTATGGGCGGTGGGCTGAAATATCCTTATGTCGAGGGATTGCGTCTGGACGAAGCAATGCAGCCGATCACACtgca < 2:831364/233‑3 (MQ=255) aTTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtgat < 1:144214/239‑1 (MQ=255) tCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑Cg < 1:163060/239‑1 (MQ=255) tCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCGGAAATATCCTTATTTCGGAGGATTGTGTTTCCGCGGAGGAATACAACCCCTCACACttat‑gaaag > 1:63743/1‑236 (MQ=255) aTGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAGATACTTATGGCGAAGGGAGTCGTCTCGACGGAACCATGAAACCGCCAACACagat‑gagccttgg > 1:485206/1‑232 (MQ=255) aTGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAg < 1:322227/238‑1 (MQ=255) aTGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGGGGAATATCCTTTTGTCGAAGGAGTGCGTCTCCACGCCGGCATGCATCCCCTCCCACCGAT‑GTG‑CGTAgg > 2:554658/1‑239 (MQ=255) tGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATCCTTTTTTCTGAGGGGTGGGTCTCCGCGCAGCAAAGCATCCCCTCTCACTGAT‑TAC‑Cgggtgggtttt > 2:695712/1‑229 (MQ=255) tGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGAAAAATACTTATGTCGAAGGATTTCGTCTCGCCGAAGCAATGCATCCGCCCCCACTGTT‑GAC‑CCTAGGGG‑GTTa > 1:885367/1‑239 (MQ=255) cGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATCCCTTTTTCGCAGGATTTCGTCTCGACGAAACAAAGCATCCCCTCACACTGAT‑GGC‑CCTAGGAG‑GTTAtt > 1:889083/1‑238 (MQ=255) ggAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGc < 1:961168/238‑1 (MQ=255) gAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCa < 2:427109/238‑1 (MQ=255) aaGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCaa < 1:1153249/238‑1 (MQ=255) tCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGCCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtgat‑gtgagcgg < 1:368676/217‑8 (MQ=255) gATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGGAATATCCTTATGTCGGAGGGTTGCGTCTCGACGAAGCAATGGATCCGCCCCCACTTCT‑TAA‑CGTAGGGG‑TTTATGGCAAGGCGGTACCg > 1:1304048/1‑238 (MQ=255) tgCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAACATACTTATTTCGAAGGGTTTGGTCGCCACGAAGCAAGGCAACCCCCCACACTGCT‑GAG‑CCTAGGGG‑GTTTTGGGAAAGCGTTATAGCCACcaaaa > 2:826643/1‑238 (MQ=255) gttGGTTTCCACCTGCACAAATTGCGGGCTC‑TGCCCAACCTTACAAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTGTGCACCGGAA‑CA‑GAGCCCAGGCCAGAGGGACCGCTTTAGCGGCGGTGGGCTGAAATATCCTTATGGCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GCC‑CGTAGGTG‑TTTATGGAAAGGTGTTACCGCCACAAAATGgcgcgc < 2:1351486/237‑1 (MQ=255) tttCCGCGGCACAAATTGCTGGCGCTTGCCGACCC‑TACCAGAAAGGCGAAGTTGGTCGCTTCCGAAACAA‑TTTATGCCCCGGAA‑CA‑GATGCCAGGCCGGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATGGCGTCTCGACGAAGCAATGCACCCGCTCACACTGAG‑GAC‑CGTGGGTG‑TTTAGGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCg < 2:711761/239‑1 (MQ=255) aaTTGCTGGCGCTTGCCGAACC‑TACCGGAAATGCGAATTATGTCGTTTTCGAAACAT‑TTTATGC‑CCGGAACCA‑GATGCCAGGCCAGCAGGCCCGCTTTACCGGGGGTGGGTTAAATTATCTTTATGTCGAGGGATTGCGTCTCGACGAAGCAAGCCATCCGCTCCCATGGAT‑GCC‑CGTAGTTT‑TTTATGGCAAGCCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCg < 2:1275795/239‑1 (MQ=255) cgctgcccGAACC‑TCCCAGAAATGCGAGGTATGGCGTTTTCAAAAAAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGCCCGTTTTATCGGGGGTGGGCTGAAATACCCTTATGTCGAAGGATGGCGTCTCGACGAAGCAATGCACCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAata < 2:1304048/232‑1 (MQ=255) cgcTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGGGGAAAAACCTTATGTCGAAGGAGTGTGGCTCGACGGAAGAATGCGTCCGCCCCCACTTAT‑GAC‑CGCAGGGG‑GTTTTTGCAAGGGGGTAACGCCCCCAAAAGGCGCGCCGGGGCggccgggtgtggcgcggggaaat > 2:772971/1‑217 (MQ=255) cgcTGGCCAACCC‑TACCAGAAAGGCAAAGTATGTCG‑TTTCGAAAAAATTTTAGGCCCCGGAA‑CA‑GATGCCGGGCCAGAAGGCCCGCGTTATCGGGGGGGGGCTAAAATATCCTTATGTCAAAGGTTGGCGTCTCGACGAAGAAATGCATCCGCTCACCCTGAT‑GAC‑CGTAGGTG‑TTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAata < 2:514380/238‑1 (MQ=255) ccgcccaatggtatgTATCTCGATTTCGAAACAA‑TTTAGGCACCGGAA‑AA‑GATGGCGGGCCAGCGGGACCCTTTTACCGGGGGGGGGTTGAAATATCTTTAGGGCGAAGGATGGCGTCTCGCCGAACCAATGCTTCCCCTCCCCCTGTG‑GCC‑CGTGGGGG‑TTTATGGCAGGTCGTGACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGAt < 2:974946/225‑1 (MQ=255) aCCAGTAATGGTAAGTAGGTCGCTTTAGAAAAAA‑TTTAGGCACCGGAA‑AG‑GAGGCCAGGCGGGAGGTACCTCTTTATGGGTGGGGGGATGAAATATCCTTAGGTGGAAGGATGGCTCCTCGACGAAGAAAGGCATCGGCTCACATTGAT‑GCC‑GGTAGGTT‑TTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGAt < 2:370213/239‑1 (MQ=255) cgcggAAGTAGTTCGTTTTCAAAAAAA‑TTTATGCACCGGAA‑CG‑TGTGCCGGGCAGGGAGGCCCGTTTGTCCGGCGGGGGTCTGAAAAATCTTTATGTCGAAGGTTTGCGTCTCGACGAAGAATTGCTTCCGCTCCCGCTGAT‑GCC‑CGTGGGTG‑TTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg < 2:145301/235‑1 (MQ=255) agtggAAGTATGTCCCTTTCAAAACAT‑TTTATGCACCGAAA‑CA‑GATCCCAGGCGAGCAGGCCCTCTTTCGCGGCGGTGGGCTGAAATATCTTTATGTCGAAGGTTTGCGTCTCGACGAAGAAATGCATCCGCTCACACTGAG‑GAC‑TGTAGGTT‑TTTATGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg < 2:615172/235‑1 (MQ=255) aTGCGAAGTATGCCGTTTTGAAAACAA‑TTTATGCACGGGAA‑CA‑GATGCCAGGCCAGCAGGACCGTTTTAGCGGGGGGGGGCTAAAATATCCTTATGTCGAAGGATTGCGTCGCGACGAAGAAATGCATCCGCTCACACTGAT‑GAC‑CGTGGGTG‑TTTAGGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg < 2:1291526/239‑1 (MQ=255) tGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGGAAAATCCTTTTTTTGGAGGGATGGGTTTCGGCGGAGGAATGCATCCCCTCCCCCCGGT‑GAG‑CGGAGGGG‑GTTTTGGGAAAGGGGTAACGCCCCCAAAAGGCGCGGCGGGGGGGCTGCTTTTTGCGGGGGAAAATTGCTTTTAAAGGGGTaaaaaga > 2:394296/1‑236 (MQ=255) tGCGAAGGATGTCGCTTTCAAAACAT‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCGGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTGGGTG‑TTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGa < 1:826643/239‑1 (MQ=255) aaGTATTGCGCTGTCTAAACAA‑TTTATCCCCCGAAA‑CA‑GATGCCGGCCCGGCAGGACCGTTTTATCGGTGGTGGGCTAAAATACCCTTATGGCGAAGGATTGCGTCTCGACGAGCCACTGCACCCGCTCACACGGAG‑GAC‑CGTAGGTG‑TTTATGCAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 2:1269567/239‑1 (MQ=255) ggTATGTCGTGTTCGAAACAA‑TTTTTGCACCGGAA‑CA‑GATGCCAGGCCGGCGGGCCCGCTTTAGCGGCGGTGGGCTAAAATATCTTTATGTCGAGGGATTGCGTCTCGACAAAACAATGCACCCGCTCACACGGAT‑GAC‑TGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 2:917108/237‑1 (MQ=255) attATGCCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATCTCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 1:1305774/236‑1 (MQ=255) aggaTGTGGCTTGCAAAACAA‑TGTATGCGCCGAAC‑CA‑GATCCCGGGCCACGGGGACCGCTTTCCCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGTTTGCGTCTCGGCGAAGCAATGCACCCGCTCACACTGAT‑GAC‑GGTGGGTT‑TTTAGGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 2:349639/235‑1 (MQ=255) aGTATGTCGCTTGCGAAACAA‑TTTATGCCCCGAAA‑AA‑GATGCCAGGCCAGGAGGCCCTCTTTATCGGCGGTGGGCTGAAATTTCTTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCACCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 1:502751/238‑1 (MQ=255) aGTATGGCGCTTTCAAACCAA‑TTTATGCGCCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTGTGGGCGGTGGGCTGAAATATCCTTATGGCGAAGGATGGCGTCTCGACGAAGAAATGCATCCGCGCCCACTGAG‑GAC‑CTAAGGTT‑TTTAGGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 1:281454/238‑1 (MQ=255) aGTATGCCGTGTTGGAAACAA‑TTTATGCCCCGAAA‑CA‑GATCCCAGGCCAGCAGGCCCGCTTTATCGGCGGTGGGCTAAAATATCCTTATTTCGAAGGATTGCTTCTCGACGAAGCAATGCACCCGCTCACACTGAT‑GAC‑CGGAGGTT‑TTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt < 2:9506/238‑1 (MQ=255) tGTCGCTTTCAAAAAAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCGGAAATATCCTTATGGCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCCCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTa < 1:806743/239‑1 (MQ=255) tttCGAAACAA‑TTTATGCCCCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTAAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGc < 1:868977/239‑1 (MQ=255) ttagaaaaCAA‑TTTATGCACC‑GAA‑CAGGATGCCAGCCCACCAGAACCGCTTTATGGGGGGTGGGCTGAAATATCTTTATGTCGAAGGATGGGGTCTCGAGGAAGCAATGCACCCGCTCACACTGAT‑GAC‑CGTGGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGc < 2:29910/234‑1 (MQ=255) tCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGCGGGAATAACCCTATGTCGAAGGACTTCGTCCCGCCGGAGGAAAGCCACCGCTCACACTGCT‑GACAGGTGGGGG‑TTTATGACAAGCGGTTACCG‑CACAAAAAAGCGCGGCGCGGCGCGCG‑GTTGTGGCGGTGGAATATTGGTTTCAAGGGGTTAAAACCAAAGTCTGCAtttaattg > 2:1127620/1‑233 (MQ=255) acGAAACAA‑TTTGTGGCCCGGAT‑GA‑GATGCCTGGCCAGGCGGACGTGTTTGTAGGGGGTTGGCAGAAATATCTTTATGAGTAGGTATCGCGTCTCGCGAAAGCAAGCCCTCCCCGCCCAGTTAT‑CCC‑CATAGGCG‑TGTATGCGAAGGCTTAACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTg < 2:53289/238‑1 (MQ=255) ccGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGGAAAATCCTTTTTTCGGAGGATTTCGTCTCCACGGAGCAAAGCATCCCCCCCCACTGTTGGAC‑CGAAGGGT‑TTTTTGGAAAGG‑GGTACCCCCCCAAAAAAGGGCGCCGGGGGGGCTG‑GGTGTGGCGGGGGGAAATGGCTTTAAAGGGGATAAATAGATAAACAGGTTTAAAGTGACCcgcgcgggttccc > 2:197477/1‑229 (MQ=255) aacgtagcccggcccagCAGGACTCTTTGAGCGGGGGTGGGCTAAAATATCCTTATGGCGAAGGATTGCGTCTCGACGAAGCAGTGCATCCGCTCACATGGAT‑GAC‑CGTAGGTG‑TTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAac < 2:63743/225‑1 (MQ=255) atgcagggccggCAGGACCGTTTTACCGGCGGTGGGCGAAAATATCCTTATGTCGAAGGATGGGTTCTCGACGAAGCAATGCACCCGCTCCCACGGTT‑GCC‑CGTAGGTT‑TTTATGGCAGGGCGTTCCCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAaccacc < 2:219254/231‑1 (MQ=255) ggTGGGCTGGAAAATCCTTATGTCGAAGGGTTGCGTGTCGACGAAGCAATGCAACCGCTCCCCCTCCT‑GAC‑CGTAGGGG‑TTTTTGGCCAAGCGGTACCGCCCCAAAAAAGCGCGCCGGGGCGGCTG‑TTTGTGCCGCGGGAATATGGCTTTATAGGGGTTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGGCCCCCAACCCCCCGGGATCTCGCAGCGGCTGACGCAAACGGgtt > 2:595253/1‑239 (MQ=255) aaaTATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGGGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCa > 2:397743/1‑238 (MQ=255) ctCCTTATGGCGAAGGATTGCGTCTCGACGAAGCAGTGCATCCGCTCCCACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGtt < 1:554658/238‑1 (MQ=255) tCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGAG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTa > 2:1025057/1‑239 (MQ=255) ctatggTCGAAGGATTGGGTCCCGACGAAGCAATCCACCCCCCCACACTGAT‑GAC‑GGTAGTTT‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAAt < 1:394296/234‑1 (MQ=255) aTGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCCCCACAAAATGGCGCGCCGGTGCGCCTG‑ATTGTGCCGTGGACATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCACGCGTCCGCCAACAACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCg > 2:324902/1‑239 (MQ=255) aaGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGtt > 2:844960/1‑239 (MQ=255) aaGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGtt > 1:1245871/1‑239 (MQ=255) tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCa > 1:292242/1‑238 (MQ=255) tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAAACACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACCTTAATCCGTATGTTGATCa > 2:368232/1‑238 (MQ=255) gcgacgaGACAATGCACCCGCTCACCCTGAG‑GCC‑GGTGGGTT‑TTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCgcgc < 2:889083/238‑1 (MQ=255) cgacgaAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCcgc > 2:568578/1‑236 (MQ=255) aaTGCATCCGCTCACCGTGAG‑GAC‑CGTAGGTT‑TTTAGGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTcaca < 1:814387/237‑1 (MQ=255) ccGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGa > 2:585763/1‑239 (MQ=255) cGCTCACATGGAG‑GAC‑GGT‑GGTGTTTTAGGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGaa < 2:485206/239‑1 (MQ=255) | GCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACC‑TACCAGCAATGCGAAGTATGTCGCTTTCGAAACAA‑TTTATGCACCGGAA‑CA‑GATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAT‑GAC‑CGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTG‑ATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAA > NC_000913/2039894‑2040362 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |