Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,139,187 T→G 22.6% V429G (GTG→GGG)  yegH → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,139,1870TG22.6% 150.1 / 8.8 69V429G (GTG→GGG) yegHinner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (40/11);  new base G (3/14);  total (44/25)
Fisher's exact test for biased strand distribution p-value = 1.29e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTATCGACCTGCTACAACAGTCTCTGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGCC  >  NC_000913/2138958‑2139420
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          
gTTATCGACCTGCTACAACAGTCTCTGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGGGGAAgaga                                                                                                                                                                                                                                   >  2:894554/1‑238 (MQ=255)
  tATCGACCTGCTACAACAGTCTCTGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGAtag                                                                                                                                                                                                                                >  1:375860/1‑237 (MQ=255)
     cGACCTGCTACAACAGTCTCTGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGGGACATTAAGTGACGTCACTAAACCCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGAc                                                                                                                                                                                                                              >  1:368084/1‑238 (MQ=255)
            cTACAACAGTCTCTGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCa                                                                                                                                                                                                                      >  1:1123700/1‑239 (MQ=255)
                      ctctGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCag                                                                                                                                                                                                            >  1:250798/1‑239 (MQ=255)
                      ctctGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCag                                                                                                                                                                                                            >  2:1385951/1‑239 (MQ=255)
                      ctctGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACGGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCag                                                                                                                                                                                                            >  1:412958/1‑239 (MQ=255)
                       tctGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGATGGTGTTCCCGGAGACCTTGCCGTTGTTACCTGCCCTGGAGCAGGTCCGTAATGCCCGCACGCTCTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGGGGGAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTACGTTCACGACAGAACGGGAAGAAAGCGAAGCCCGCCATGATATTAaga                                                                                                                                                                                                           >  2:1060686/1‑239 (MQ=255)
                               gcgAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAACAGATCGACGCCCGCCATGATATTCAGAAGAATGCg                                                                                                                                                                                                   >  2:869408/1‑239 (MQ=255)
                                                  gtggttggttcgcggccggtgggTGTTCCCGGAACCTTTGCGTTTGTTCCCTGCCCGGGGGCGTTCCGGTAGGGCCCGCCGGCTTTTTGTTTTTGTGTTGGTGGAGTTTGGCTGGTGGGAGGGATTTTTGAAATTAGGTGAGGTCACGGAAACCATTGCTGGTACCTTACCGAAGGAGGGGAAAGGGATGGCGGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCg                                                                                                                                                                                 <  2:625427/217‑1 (MQ=255)
                                                  ggTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGa                                                                                                                                                                                >  1:1328025/1‑239 (MQ=255)
                                                           tcgctcgCCGCTGGGTTTCCCGAAACCCTTGCGTTTGTTACCTGCCCGGGGACAGTCCCGTAATGCCCGCCCGCTTTTTGTTTTTGTGGTGGATGAGTTGGGCTCGGAGGAAGGGTTTGTGCCATAAGTAGGGTTCTCGGAACCAATTCCCGATAACTCACCAAAGAATGTGAAAGAGATCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCata                                                                                                                                                                       <  2:1123700/233‑1 (MQ=255)
                                                                   cGCTGGTGTTCCGGAAACCCTTGCCTTTGTTTCCTGCCTGGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACCTCACTAAACCCATTGCCGGTAACTTACCGAACGAAGGGAAAGAGATCGCcgcccg                                                                                                                                                                                                                         <  2:1092206/181‑1 (MQ=255)
                                                                      tggttctCCCGGAAGCCTTGCCGTGTTTACTTGCCCTGGCGCAGTCCCGTATGGCCCTCACGCATTTTTTTTGTGGGGTGGATGATTTTGGCTTGGGGAAAGGGATTGTACCATTAAGGGACGCCACTGAAACCATGGCCGTTATCTTCCCGAAGGAAGGGGAAGACCTGGCGGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTgga                                                                                                                                                             <  2:375860/232‑1 (MQ=255)
                                                                      tGGTGTTCCCGGAACCCTTGCCGTTGTTACCTCCCCTGGAGCATTTCGGTAATGCCCGCCCGCACTTTGTTTTTGTGGTGGATGAGTTGGGCTCGGTGGAAGGGATTGTGACATTAAGTGCCGTCACTGAACCCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTgga                                                                                                                                                             <  1:31799/238‑1 (MQ=255)
                                                                         tgctCCCGGACCCCTCGCCGTTGTTACCCGCCCGGCACAATTTTGTTCATCCCGGCTCGCACTTTTCTTTTGTGGTCGTTGAGTTTGGCTCGGTGGAGGGGTTTGTGAAATAAAGTGACGTTACTGAAACCATCGCCGGTAGCTTCCCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGAt                                                                                                                                                         <  2:1006422/236‑1 (MQ=255)
                                                                             cccGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATAtt                                                                                                                                                                                                               >  1:1092206/1‑181 (MQ=255)
                                                                                                  aCCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGtgccgctgcc                                                                                                                                  >  2:922200/1‑237 (MQ=255)
                                                                                                          tGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGACGGGGAAGAGATCGACGCCGGCCATGACATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAGTGGTCATATGCCGCTGCAGGGTCGGGTGCAATATGTGCCGCTTCCGCTGGCTGaa                                                                                                                        >  2:1250250/1‑239 (MQ=255)
                                                                                                               cattcccGTAATGCCCTCTCTTACTTGGTTTTTGGGGTGGATGATTTGTGCGGGGGAGAGGGGTTGGTGATATAAAGTACCGCCACTGAACCCTGTGCCGGTAACTTACCGAAGGAAGTGGAAGAGACCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAAc                                                                                                                    <  2:588033/233‑1 (MQ=255)
                                                                                                                tgTTGCGTACGGCCCGCAGCATTTTGGTTGTTGGGGGGGATGGGTGGGGCTGGGGGGAAGGGATTGTGACATAAAGAGACGTCACAGAACCCATTGCGGTAAACTTCCGGAAGGAGGGGGAAGAGACCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGt                                                                                                                  <  2:385412/238‑1 (MQ=255)
                                                                                                                 gTTCCTTAATGCCCGCTCGCATTTTGTTTTGGTGGTGGAGTATTTTGCCTCGGGGGAAGGAATTGCAAAAGTAAGGGCCGCCACAGAAACCTCCGCCGTTAACTCACCAAACGAGGGGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGt                                                                                                                  <  2:15352/238‑1 (MQ=255)
                                                                                                                 gTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTg                                                                                                                 >  1:888842/1‑239 (MQ=255)
                                                                                                                 gTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTg                                                                                                                 >  2:287423/1‑239 (MQ=255)
                                                                                                                  ttCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGa                                                                                                                >  2:134938/1‑239 (MQ=255)
                                                                                                                       gTAATGCCAGCGCGCTGTTCTTTTTGGGGGGGGATGGGTCTGTCTGGGTGGAATAGATGGTGCAATTAGGTGACATGACAAAAACATCTGCCGGTAACTAACCGAACGAGGGAGAAGCGACCAACCCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAatat                                                                                                            <  2:791203/239‑1 (MQ=255)
                                                                                                                             cccGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACAcc                                                                                                      >  2:1308903/1‑239 (MQ=255)
                                                                                                                             cccGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACAcc                                                                                                      >  1:849694/1‑239 (MQ=255)
                                                                                                                               cgcacgcaCTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACAAt                                                                                                    >  2:964841/1‑239 (MQ=255)
                                                                                                                                  acgcacTTTGCTTTTGTGGTGGATGCGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGATCCTGGACGACGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTg                                                                                                  >  1:1257346/1‑238 (MQ=255)
                                                                                                                                  acgcacTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAata                                                                                                                                              >  1:885481/1‑194 (MQ=255)
                                                                                                                                   cgctttttGTTTTTGTGGTGGATGGGTTGGGCGGGGTGGAAGGATTTGGAACTTTAGGTACCGTCAATGAAACCTTGCCCGGTAACTCACCGAAGGAAGGGGAAGAGATCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGcc                                                                                                <  2:888842/234‑1 (MQ=255)
                                                                                                                                           tgttgtggtggggGAGGGGTTTGGCGGGGGGAAAGGGATTGTGAAATAAAGGGGCGCCACAAAACCCAGTCCGTGAAACTCACGAAAGGAGGGGAAATAGAGCGCGCCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTAATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGt                                                                                         <  2:1238199/232‑1 (MQ=255)
                                                                                                                                            cTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTg                                                                                       >  1:1390357/1‑239 (MQ=255)
                                                                                                                                              tttgggggtggATGAGTTGGGCTGGGGGGAAGGGATTGTGAAATTAAGGGACGTCACGGAAACCTTGGCGGTTAACTTCCGAAAGGAAGTGGAAGAGACCGCCGCCCGCCATGATATTCAGAAGAATGCGGa                                                                                                                                                                                                 <  2:312769/126‑1 (MQ=255)
                                                                                                                                              tttGGGGTAGTTTAGTTTGGCGCGGAGGAAGGAATTGTGAAATTAAGTGACGTTATTGAAACCTTTGCCGGTAACTTACCGAACGAAGGGGAAGATATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGAt                                                                                     <  1:968024/239‑1 (MQ=255)
                                                                                                                                                 tggggtggATGAGTTTGGCGCGGTGGAGGGTTTTGTGACATTAAGTGACGTCGCTGAAACCATTGCCGGTAACTTCCCGAACGAAGGGGAAGAGATCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATgg                                                                                   <  1:221038/236‑1 (MQ=255)
                                                                                                                                                     gtggATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAata                                                                                                             >  1:1193266/1‑209 (MQ=255)
                                                                                                                                                        gATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTgg                                                                                                                                                                                       >  1:312769/1‑132 (MQ=255)
                                                                                                                                                               ttGGCGCGGTGGAGGGTATTGGGACATTAAGGGACGTCACTGAACCAATTGCGGGTAACTTACCAAACGAAGTGGAAGAGTTGGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCg                                                                      <  2:1328025/238‑1 (MQ=255)
                                                                                                                                                                tGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTa                                                                    >  1:905771/1‑239 (MQ=255)
                                                                                                                                                                tGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTa                                                                    >  1:386208/1‑239 (MQ=255)
                                                                                                                                                                       gTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGAGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGGCGGCGAATGGTCATATGCCGCTGGAGGATCTGGGGCCATATGTGCCGCTGCCGCTGGATGACAAACGTGAATATCCCACCATTGCGGGGCTGTTGATGGAGTATTTGCAGCGTATTCCaga                                                             >  2:866261/1‑237 (MQ=255)
                                                                                                                                                                                  ttGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaa                                                   >  2:1187327/1‑239 (MQ=255)
                                                                                                                                                                                   tgtgACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCTTGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaag                                                  >  1:919840/1‑239 (MQ=255)
                                                                                                                                                                                   tgtgACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaag                                                  >  2:695997/1‑239 (MQ=255)
                                                                                                                                                                                   tgtgACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaag                                                  >  1:89382/1‑239 (MQ=255)
                                                                                                                                                                                   tgtgACATTAAGCGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaag                                                  >  1:39321/1‑239 (MQ=255)
                                                                                                                                                                                   tgtcACATTAAGGGACGTCACTAAACCCATGGCCGGTAACTTACGAAACGAAGTGGAAGAGACCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCgaag                                                  <  1:894554/239‑1 (MQ=255)
                                                                                                                                                                                         aataaagggccgccacgaaaaCCTTTCCCGTTAACTTCGCGAAGGAAGTGGAAGAGAGCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGtt                                             <  1:869408/221‑1 (MQ=255)
                                                                                                                                                                                         aTTAAGTCACGTCGCTGAAACCATTGCCGGTAACTTACCGAGCGAAGTGAAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTc                                            <  2:394960/239‑1 (MQ=255)
                                                                                                                                                                                          ttAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCa                                           >  2:932422/1‑239 (MQ=255)
                                                                                                                                                                                          ttAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCa                                           >  2:451120/1‑239 (MQ=255)
                                                                                                                                                                                           tAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAg                                          >  2:796527/1‑239 (MQ=255)
                                                                                                                                                                                                  aCGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTgggg                                    >  1:563121/1‑239 (MQ=255)
                                                                                                                                                                                                    gTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAatat                                                                                                            >  2:1312344/1‑165 (MQ=255)
                                                                                                                                                                                                    gTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAatat                                                                                                            <  1:1312344/165‑1 (MQ=255)
                                                                                                                                                                                                    gTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTgggggt                                  >  2:810547/1‑238 (MQ=255)
                                                                                                                                                                                                       aCTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGatta                               >  2:1252602/1‑239 (MQ=255)
                                                                                                                                                                                                        cagAAACTTTTGCGGGTACTTTCCCGAACGAAGGGGAAGAGAGCGCCGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGattat                              <  1:1269079/237‑1 (MQ=255)
                                                                                                                                                                                                        ataaacccATGGCCTGTACTTTACCAACCAAGGGGAAAGAGTGCGCCCCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGattat                              <  1:246400/233‑1 (MQ=255)
                                                                                                                                                                                                            acccATTGCGGGTAACTTACCGAAAGAGGGGGAAGAGATCGACGGCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGattat                              <  1:1362672/233‑1 (MQ=255)
                                                                                                                                                                                                                 ttgcagatacctgcccGAACGAAGGGAAAGGACTCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTaaa                     <  1:1208154/225‑1 (MQ=255)
                                                                                                                                                                                                                 ttGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTggggg                                   >  1:101455/1‑225 (MQ=255)
                                                                                                                                                                                                                 ttGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTaaa                     >  2:202670/1‑239 (MQ=255)
                                                                                                                                                                                                                  tcccGATAACTTGCTGAACGAAGTGGAAAGGGTCCACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGTAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTaaa                     <  1:1385951/236‑1 (MQ=255)
                                                                                                                                                                                                                  tGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTaaa                     >  1:912377/1‑238 (MQ=255)
                                                                                                                                                                                                                                  aacgaaGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGaaa     >  1:1134725/1‑238 (MQ=255)
                                                                                                                                                                                                                                    cgaaGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGcc  >  2:140472/1‑239 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          
GTTATCGACCTGCTACAACAGTCTCTGCGCGGCGAACCGCTCAACCTGCGGGTGTTGATTCGCCAGCCGCTGGTGTTCCCGGAAACCTTGCCGTTGTTACCTGCCCTGGAGCAGTTCCGTAATGCCCGCACGCACTTTGCTTTTGTGGTGGATGAGTTTGGCTCGGTGGAAGGGATTGTGACATTAAGTGACGTCACTGAAACCATTGCCGGTAACTTACCGAACGAAGTGGAAGAGATCGACGCCCGCCATGATATTCAGAAGAATGCGGACGGTTCCTGGACGGCGAATGGTCATATGCCGCTGGAGGATCTGGTGCAATATGTGCCGCTGCCGCTGGATGAAAAACGTGAATATCACACCATTGCCGGGCTGTTGATGGAGTATTTGCAGCGTATTCCAAAACCTGGCGAAGAAGTTCAGGTGGGGGATTATTTGCTTAAAACGTTGCAGGTAGAAAGCC  >  NC_000913/2138958‑2139420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: