Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,691 C→T 20.4% intergenic (+45/+20) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6910CT20.4% 52.1 / 2.6 33intergenic (+45/+20)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (15/11);  new base T (5/2);  total (20/13)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

AGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151504‑2151924
                                                                                                                                                                                             |                                                                                                                                                                                                                                          
aggaaaaaacaaaaaaaaaaGATATTTCAGAGTGGATGTATACGCGGGGGGAAAAAATTTACGGGGTTTATACCATTTATCCTTTATTGGTCCCCTATCAAAAAGAAGAAGCCGAGGAGTAAAGAGAAAATTTAGTTC‑GCAAATCAGCCGTTATATTTATTGTTTAAC‑CGGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt                                                                                                                                                                                         <  1:362326/224‑1 (MQ=255)
     aaaaaaaaaaaagaaAATATTTCAGATTGGATGTATACGCGGGGGGAAAAAATTTAGGGCGGTTATCCCATTGATCTTTTATGGGTCACCTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTC‑GCTAACCAGCCGTTATATTTATTGTTTAAA‑CGGGCGGTGGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc                                                                                                                                                                                    <  1:829289/229‑1 (MQ=255)
                      tatTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCAATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGtt                                                                                                                                                                  <  1:756705/239‑1 (MQ=255)
                                                                            tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTTTTTTCTTC‑AACCGCCATGAGAAAATCACTCTGCCCACAGCGCCCGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACCAAAACTGCTTTTACAATTCAGGTAATAAAACGAgttg                                                                                                            >  2:864063/1‑239 (MQ=255)
                                                                            tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTTTTTTC‑AAACGCCATAAGAAAATCACTCTGCCCACAGCGCCAATGCCCAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCCGGACACAAAACGCTTTAACAATTTCGGGAATAAATCGAgttg                                                                                                            >  1:1282206/1‑239 (MQ=255)
                                                                            tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttg                                                                                                            >  1:394252/1‑239 (MQ=255)
                                                                                   ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTTCGGGAATAAATCGAGTTGTTCCTGc                                                                                                     >  1:885751/1‑239 (MQ=255)
                                                                                         gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTTATTGTTGTCTTC‑AACACCCCTGAGAAACACACTATGTCCACAACGCCCGTGACAACGGTTTATCTTCCTCCGCTCCCTCGTTTACCGCCCGGCTAGACAAAAACTGCTTTCACCATTCAAGTAATAAAATGAGTTGTTCCTCCgct                                                                                                  >  2:713090/1‑234 (MQ=255)
                                                                                         gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTAATTGTTTTTTTC‑AAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTTTCATCATTCGTACCCTCGTTTACCCCCCGGCTGGACAAAAACTGATTTAACAATTCAGGTAATAAATTGAGTTGTTTCTGCGTtgct                                                                                               >  1:109452/1‑239 (MQ=255)
                                                                                            aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGGGCCAGTGCCCAAGTTTTATCATTATCCGTACGCTCGTTTACCGCCGGGCTGGACCAAAAATGCTTTAACAATTCAGGGAATAAATCGAGTTTGTTCTGCGTtgtttcc                                                                                            >  1:862143/1‑234 (MQ=255)
                                                                                                aacaaaaaaaaaaaGCCGACGAGTAAAGAAAAAAATTATTTC‑TATAATCCGCCGTTATTTTTTTTGTTAAAC‑CGGGCGGTGAAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCa                                                                                        <  1:184393/235‑1 (MQ=255)
                                                                                                   cAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTTAATTGTTTTTTTT‑AACCCCCATGAGTAAACCACTCCTCCCACAGAGCCACTTCCCACGTTTTTTCATCATACGCACGCTCGTTTACCGCCGGGGCGGGCAAAAACCACCTTTAAAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTTCTgccgcccct                                                                                      >  2:1359688/1‑235 (MQ=255)
                                                                                                      aagaagaagCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCCTGAGAAAAACACTCTGCCCCCAGCGCCAGGGGCAACGTTTTTTCATCATCCCTACGCCCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAAAAATTCAGGTAATAAAACCAGTTTGTCCTGCGTTTCTGCCGCCAGTccct                                                                                  >  2:1203337/1‑237 (MQ=255)
                                                                                                           gaagCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTTTTC‑AACCGCCCTGAGAAAATCCCTCTTCCCACAACGCCAAGGGCAACGTTTTATTATCATTCCAACGCCCGTTTTCCCCCGGGCTGGGCAAAAAATGCTTTAAAAATTCAGGTCATAAATCGAGTTGTTTCTCCGTTTCTGGCGCCCGTCCATTGtaa                                                                             >  2:85810/1‑239 (MQ=255)
                                                                                                           gaagCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAAATCAGGTAATAAATAGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTTaaa                                                                             >  2:1296350/1‑239 (MQ=255)
                                                                                                           gaagCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGAAAAAACTGCTTTAACAAATCAGGTAATAAATCGAGTTGGTCCTGCGTTTCTGCCGCCAGTCCATTTaaa                                                                             >  1:1395009/1‑239 (MQ=255)
                                                                                                                        tAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCCGGCTGGGCAAAAACTGCTTTAACAATTCCGGGAATAAATCGAGTTGTTCCTTCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaaga                                                                >  2:45566/1‑239 (MQ=255)
                                                                                                                          agaaCAAAATT‑GTTCTGCTAATCAGCTTTTTTATTTTTTTTTAAAC‑CGGGCGTTTGATGTGTGTTCTTA‑ACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaa                                                              <  2:454590/239‑1 (MQ=255)
                                                                                                                            agCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAAATCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTaaagaaag                                                             >  1:182505/1‑238 (MQ=255)
                                                                                                                                aaattttgtccctaaaTCAGCTTTTTTTTTTTTTG‑TTAAA‑TGGGGGGTTTGATTTTTTTTTTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                                         <  1:48619/225‑1 (MQ=255)
                                                                                                                                 aaTTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGca                                                          >  1:306779/1‑236 (MQ=255)
                                                                                                                                    tggTTC‑GCTAATCAGCCGTTATATTTATTTTTAAAA‑CGGGGGGTGAAATTGTTTTCTTC‑ACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  1:879522/237‑1 (MQ=255)
                                                                                                                                    tatttC‑GTTAATCGGCCGTTATTTTTTTTTTTAAAG‑CTGGCGTTAGAATGGTTTCCTCC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  1:864063/236‑1 (MQ=255)
                                                                                                                                      tgttcgtcaaaccagctttATTTTTTTTGTTTAAACCGGGGGGTAGATTTGTTGTTCTA‑ACCCGCCATGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTCCGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  2:306779/222‑1 (MQ=255)
                                                                                                                                      gTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTTTTC‑AACCGCCATTAGGAACTCACTCTGCCCCCAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTTAGGTAATAAATCGAGGTGTTCCTGCGTTGCTGCCGCCCGTCCCTTGAAAAACGGGGGAAAGGAAGGcaaactagga                                                  >  2:830084/1‑231 (MQ=255)
                                                                                                                                                    ctgTTATTTTTATTTTTTAAC‑TGGGGGGTTAAATTGTTTCCTCA‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGc                                      <  1:190352/236‑1 (MQ=255)
                                                                                                                                                    cgtttATATTTATTTTTAAAA‑CTGGCGGTTGAATTTTTGTCTCC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                     <  1:1296350/236‑1 (MQ=255)
                                                                                                                                                     ctttATTTTTTTTTTTTAGA‑CTGGGGGTTGTATTTTTGTCTCA‑CACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa                                     <  1:327670/236‑1 (MQ=255)
                                                                                                                                                       ttATATTTATTGTTTAAA‑CTGGCGGTTTCATTGTTGTCCTT‑AACCCCCATGAGAAAATCACTCTCCCCCCAGCGCCAGTGCCCACTTTTTATTATCATCCCTACGCTCGCTTTCCGCCCGGCAGGATAAAAACTTTTTTAACAATTCCGGGAATAAATCAAGTTGTTCCTGCGTTTCTGCCGCCAGTCCATTGCAAAAAGGGGTAAAGAAAGGGACATGAGGAGAATTAtaccgacaat                                  >  1:850208/1‑230 (MQ=255)
                                                                                                                                                       ttATATTTATTGTTTAAA‑CTGGCGGTTGTATTGTTGTCTTC‑AACCGCCCAGAGAAACTCACTCTGCCCACAGCGCCCGTGCCAACGTTTTATCAACAACCGTACGCTCGTTTAACGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTTCGTTGCTGCCGCCAGGCCATTTAAAAACGGGGTAAAGAAAAGCACATGAGGAGAATTTTCCACCatat                                  >  1:1074491/1‑239 (MQ=255)
                                                                                                                                                                                tttgttttgttgTCTCA‑AACCGCCATGAAAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCa           <  1:692740/232‑1 (MQ=255)
                                                                                                                                                                                           gtCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCGGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  2:992802/1‑236 (MQ=255)
                                                                                                                                                                                           gtCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  2:1084957/1‑236 (MQ=255)
                                                                                                                                                                                             |                                                                                                                                                                                                                                          
AGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTC‑GCTAATCAGCCGTTATATTTATTGTTTAAA‑CTGGCGGTTGAATTGTTGTCTTC‑AACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151504‑2151924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑