BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	337,487	T→C	12.9%	L237P (CTC→CCC)	yahF →	putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	337,487	0	T	C	12.9%	201.3 / 2.4	109	L237P (CTC→CCC)	yahF	putative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand): ref base T (37/47); new base C (9/7); total (50/59)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACA  >  NC_000913/337252‑337724

gCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑Cg                                                                                                                                                                                                                                                   <  2:1345397/238‑1 (MQ=255)

     cGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCAACGGCGGGCGGG‑AACTGGACGAGATAATAAGCGGCCTGCTGAATCT‑CGCCG‑CCa                                                                                                                                                                                                                                            >  1:73788/1‑239 (MQ=255)

             ggCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGGGCGGGGAAATCGGCGGCCTGCTGATGCT‑CCACG‑CCCCCggggt                                                                                                                                                                                                                                     >  1:366610/1‑236 (MQ=255)

                tgctgATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGGGGAAATCGGCGGCCTGGTGATGCT‑CCACG‑CCATCGGGAGGC‑Tg                                                                                                                                                                                                                                >  1:1105895/1‑239 (MQ=255)

                tgctgATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑AACCGAGCGAGAAAATTGGCCGTCTGGTGATGGT‑CGCCG‑CCacctgggatcgg                                                                                                                                                                                                                                 >  1:1115284/1‑228 (MQ=255)

                   ggatgatGGTGCCAGACTGTAGGACGTGGATTAACAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGa                                                                                                                                                                                                                              <  2:863489/237‑1 (MQ=255)

                        atgGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCG‑GACCGGGCGGGGAAATCCGCGGCCCTGTGATGGC‑CGGCG‑CCACCGGGGGGC‑TGGGAAAAGa                                                                                                                                                                                                                        >  2:1246170/1‑239 (MQ=255)

                            ggCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCGTCgagtttatttgcacgcgtgg                                                                                                                                                                                                                      <  2:1034141/238‑20 (MQ=255)

                              ccAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGc                                                                                                                                                                                                                  <  1:1082625/238‑1 (MQ=255)

                               cAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑GACTGGGCGGGGAAATTCGCGGGCTGGTGGGGCT‑CGACG‑CCCTCGGGGTGG‑GGGGAAACggaccccaa                                                                                                                                                                                                                 >  2:140365/1‑231 (MQ=255)

                               cAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCa                                                                                                                                                                                                                 <  1:644394/238‑1 (MQ=255)

                                  aCTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGa                                                                                                                                                                                                                                                  <  1:664945/205‑1 (MQ=255)

                                      tgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑gaa                                                                                                                                                                                                        >  1:1044309/1‑239 (MQ=255)

                                      tgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGGGGGAGAAAATCGGCGGGCTGATGCTGGT‑CGACG‑CCCTCGGGAAGC‑TGGGAAAAGAA‑CCCCAAACTG‑aaa                                                                                                                                                                                                        >  1:786740/1‑239 (MQ=255)

                                          aCGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑a                                                                                                                                                                                                    <  1:515841/238‑1 (MQ=255)

                                          aCGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑a                                                                                                                                                                                                    <  1:1214108/238‑1 (MQ=255)

                                            ggCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGGGAAAAATGGCGGCGTGGTGAGGTC‑CGCCG‑CCACCGGGGGGC‑TGGGAAACCAC‑ACGCAAACTG‑CAATC‑Ctt                                                                                                                                                                                                  >  2:333814/1‑238 (MQ=255)

                                              cGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑GACTGGGCGGGGAAATCGGCGGGCCGGTGATGGC‑CGAGG‑CCATACGGGTGG‑TGGGAAAACAT‑CCGCAAAcaggaaaccttgt                                                                                                                                                                                                  >  2:326471/1‑226 (MQ=255)

                                                aTTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGGGAGAAAATCGGCGGCCTGATGAAGGT‑CGGCG‑CCATCGGGGTGC‑TGGGAAACGAT‑CCGCAAACTG‑AAATC‑ATTTCGCt                                                                                                                                                                                             >  1:967740/1‑239 (MQ=255)

                                                aTTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGCCG‑CCACCGGGGTGC‑TGGGAAACGAT‑CCCCCAACTG‑AAATC‑ATTTCGCt                                                                                                                                                                                             >  1:133550/1‑239 (MQ=255)

                                                 ttATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCaa                                                                                                                                                                                                                <  1:793321/221‑1 (MQ=255)

                                                 ttATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCt                                                                                                                                                                                             <  2:1158768/238‑1 (MQ=255)

                                                 ttATCAACGGCGCGGCGCTCTGTTGTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCt                                                                                                                                                                                             <  1:333814/238‑1 (MQ=255)

                                                          gcggggcgcTCTTTTTGGGTAACGCCGTGGGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCaa                                                                                                                                                                                    <  2:1080712/238‑1 (MQ=255)

                                                          gcgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGGC‑AACCGGGCGGGAAAATACGCGGGCCGAATGTGCC‑CCGCGCCCACCGGGTTGG‑TGGAAAAGAAC‑CCCCAAACTG‑CAATC‑ATTGCCCtttcccccc                                                                                                                                                                                     >  2:549062/1‑231 (MQ=255)

                                                           tgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGTTATTGTTGCCGCATCCGGCGCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCaaa                                                                                                                                                                                   <  1:543379/237‑1 (MQ=255)

                                                           cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGAG‑ACCTGAGGGAGGAAAACGGGGGCGCGAGGGTGCT‑TGACG‑CCCACGGGGAGG‑TGGGAAAAAAA‑CCGCCAACTG‑TAGAC‑ATTTCGCTCATTTCCaca                                                                                                                                                                                   >  2:802727/1‑236 (MQ=255)

                                                           cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGGCTGATGATGCT‑CGACG‑CCCTCGGGGTGC‑TGGGAAAAGAT‑CCGCAAACTG‑GAATC‑ATTTCGCTTATCTCCcaa                                                                                                                                                                                   >  1:970918/1‑238 (MQ=255)

                                                                    cggTTTTGGTAACGCCGTGCGTCGCGGCAACAGCGGTATTGTGGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACcgcctgcgc                                                                                                                                                                         <  2:1323327/237‑1 (MQ=255)

                                                                         ttGGTAACGCCTTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCCCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAGACGGT‑CCGCACACTG‑AAATC‑CTTTCGCTTATCTCCAAACCGcctgcgcctg‑cg                                                                                                                                                                   <  1:155260/239‑1 (MQ=255)

                                                                         ctGGGAACGGCGTGCGTCCCGGCACAATCTGTCTTGTTGGCGCACTCGGCCCCGGCGGTCAGGGGTTGAGCGTCCGCATTCGTGAATTGGGCGGCGGCGTTTCGCAACTGATGGCCTCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGAGGATGCT‑CGACG‑CCAGCGGGATGC‑TGGAAAACGTT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGcctgcgcctg‑cg                                                                                                                                                                   <  2:1370518/238‑1 (MQ=255)

                                                                          tGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGGGCGAGAAAAACCGCGGCCAGCTGGTGGT‑CGACG‑CCATCGGGGTGC‑AGGGAAAAGAG‑CCGCAAAACG‑AGAAA‑ATTTGGCTCTTCTCCAAACcgcccgctcccccgg                                                                                                                                                                   >  2:964126/1‑234 (MQ=255)

                                                                               aCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGGGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAAACGCCTTCGCCTG‑CGGTGGc                                                                                                                                                              >  1:399547/1‑238 (MQ=255)

                                                                               aCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCt                                                                                                                                                                                                                                                      >  1:219851/1‑157 (MQ=255)

                                                                               aCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCt                                                                                                                                                                                                                                                      <  2:219851/157‑1 (MQ=255)

                                                                                 gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCG‑ACCTGAGCGAGGAAATTGGCGGGCCGGTTATGCT‑CGCCG‑CCCGCCGGGAGC‑TGGGAAAAGAT‑GCGCAAAACA‑TAATCAATTCCGCTTTTTTCC‑AACcgcctgccccctcggggggcc                                                                                                                                                             >  2:75138/1‑227 (MQ=255)

                                                                                 gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGAACCGGGGGGGGAAAAT‑GGGGGCCCGGTGATGCC‑CGACG‑CCCCCGGGCTGCGGGGAAAACGAC‑CCGCAAACTA‑AAACA‑ATTGCCCTT‑TCTCCAAACcccccccccccgccgggggcc                                                                                                                                                             >  2:829232/1‑221 (MQ=255)

                                                                                 gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTTAGCGAGAAAAACCGCGGCCCGATGATGCT‑CGGCG‑CCATCGGGGGGT‑TGGGAAACAAA‑ACGCAAACTG‑AAATC‑ATTCGGCCTTTCTCCACAAcgcctgccccgtagcggggcc                                                                                                                                                             >  2:913142/1‑227 (MQ=255)

                                                                                 gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCAGAGCGAGGAAATCGGCGGCCTCGTGATGCG‑CGACA‑CCATCGGGGGGG‑TGGGAAACGGG‑CACCCAACTG‑AAATC‑GTCGCGCTTTTCTCCACACACCCCGCGCCTG‑CGCGGGccc                                                                                                                                                            >  2:689815/1‑238 (MQ=255)

                                                                                 gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATCGGCGGGCTGGTGGTGCG‑CGGCG‑CCAACGGGGAGG‑CGGAAAACGAA‑CCCCAAACTG‑AAAAC‑CATGCGGTTTTCTCCCAAACCCCTGCCCCCG‑CGGGGGgcc                                                                                                                                                            >  2:586539/1‑238 (MQ=255)

                                                                                 gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATAGGCGGCCTGATGATGCG‑CGGCG‑CCCCCGGGAGGC‑TGGGAAAAGAA‑CCGCCAACTG‑AAAAC‑CATGCGCTTATCTCCAAAACCCCCGCGCCTG‑CGGTGGgcc                                                                                                                                                            >  2:1240166/1‑238 (MQ=255)

                                                                                   cgtggtttggggCAACATCGGAATTGTGTGCGCATCCGGCATCGGCAGTCAGGAGTGGAGCGTCCGCATTAATGAATTTGGCGGCGGCGTTTCCCAACGGATTGGCACCGGCGGGCGCG‑CCCTGAGCGAGAAAATCGGCGGCCGGATGAGGCG‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAAGC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  2:1115284/229‑1 (MQ=255)

                                                                                   cgtgcgtgGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCAGGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  2:1249863/239‑1 (MQ=255)

                                                                                   cggggggcgcgGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTGGAGCGTCCGCATTCATGAATTGGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  2:164330/232‑1 (MQ=255)

                                                                                   cGTGCGTCGGGGCAGCATCGGTATTGTTGGCGCATACGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAAGTGGGCGGCGGCGTTTCGCACCGGATGGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAAGCGGCGGCCGGATGGTGCG‑CGACG‑CCAGCGGGATGC‑TGGAAAACGAG‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCGCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  2:1162367/239‑1 (MQ=255)

                                                                                   cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑CCCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  1:995100/239‑1 (MQ=255)

                                                                                   cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  1:140365/239‑1 (MQ=255)

                                                                                   cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  1:1399831/239‑1 (MQ=255)

                                                                                   cGTGCGGCGCGGCAACATCGGTATTGTGGGGGCATCCGGGACCGGCAGTCAGGAGTTGAGCGTCCGCATGCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACGG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                         <  2:1328212/239‑1 (MQ=255)

                                                                                     tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTTCGCTTATCTCCAAACCCCCCGCGCCCG‑CGGTGGGCCGCaca                                                                                                                                                       >  2:71906/1‑237 (MQ=255)

                                                                                     tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATAGGCGGCCTGATGGTGGTCCGCCG‑CC‑TCGGGGGGC‑CGGGAAAACAC‑CCCCGAAATG‑CAATC‑ACTGCGCTTTTCTTCCCACCGCCTGCGCCTC‑GGGGGGccccgcaa                                                                                                                                                       >  2:701696/1‑234 (MQ=255)

                                                                                      gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCaaa                                                                                                                                                       <  1:862663/238‑1 (MQ=255)

                                                                                      gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCaaa                                                                                                                                                       <  1:75138/238‑1 (MQ=255)

                                                                                                          tttttgGCGCATCCGGCGCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGcc                                                                                                                                  <  1:1236747/236‑1 (MQ=255)

                                                                                                          ttgttgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGcc                                                                                                                                  <  1:1240166/239‑1 (MQ=255)

                                                                                                             ttgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACACGGGCGCGAAAAACAGCGGCCTGGTGTTGCT‑CCGCC‑CCCTCCGGAGGG‑TGGAGAACGAT‑ACGCCAACTG‑AAATC‑ATTTCCGTTTTCTCCCAACCGCCCCCCCCTC‑CGCGGGGCCGCAAAggccctggccgaggcccccgccg                                                                                                                                >  2:250920/1‑216 (MQ=255)

                                                                                                              tgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCG‑ACCTGAGGGAGGAAATCGGCGGCCTGGTGGTTGG‑CGACG‑CCCCCGGGGTGC‑TGGGAAACGAA‑CCGCCAACTG‑AAATC‑ATTGAGCTTTTTTCCCAACAGACCGCGGCCG‑CGGGGGGCCGCCAAGTGGTGGAACGTGGGCGggccggcc                                                                                                                              >  2:872020/1‑239 (MQ=255)

                                                                                                                 cgcATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAACC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGc                                                                                                                            <  1:71906/238‑1 (MQ=255)

                                                                                                                  gcATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGAGCGAGAAAAACGGGGGCCTGATGCTGCT‑CGACG‑CCCCCCGGGTGC‑TGGGAAAAGATACCCCAAAC‑G‑GAATC‑ATTGCGCCTATCTCCCCACCCCCCGCGCCTGCCGGGGGCCGCCAAAGGCCTGG‑ACGTgcccgccgcccgcgcaa                                                                                                                          >  2:208109/1‑228 (MQ=255)

                                                                                                                    accgggcacgggcaGTCGGGAGTTGAGCGTCCGAATTCATGAATTTGGGGGCGGCGTTTCGCAAGTGATGGGCACCGGCGGGCGGG‑CCCTGAGCGAAAAAAGCGGCGGCCGGATGATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATGGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGc                                                                                                                        <  2:1293963/235‑1 (MQ=255)

                                                                                                                     tCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGTGCC‑GCCGCagaaca                                                                                                                      <  1:992012/239‑6 (MQ=255)

                                                                                                                     tCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGGTGC‑TGGAAAACGAT‑CCCCAAACTG‑AAATC‑ATTGCGCCTATCTCCAAACCGCCTGCGCCTG‑CGGGGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCCGCCGCCAGcc                                                                                                                       >  1:492227/1‑239 (MQ=255)

                                                                                                                       gggagcgggcaGCCAGGAGTTGGGGGTCCGCGTACATGAGTTGGGCGGCGGCTGTTGCCAACTGATTGCCCCCGGCGGGCTGG‑CCCTGAGCGAGAAAACCGGGGGCTTAATGAGGCT‑CGACC‑CCATCGGGATGC‑TGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCgg                                                                                                                     <  2:1021468/232‑1 (MQ=255)

                                                                                                                            ccggcgGTCAGGAGTGGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTCCGCAACTGATTGGCCCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGgtc                                                                                                                <  1:802727/239‑1 (MQ=255)

                                                                                                                                                gTCCTCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTgg                                                                                            <  1:1179859/239‑1 (MQ=255)

                                                                                                                                                gTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCACCTGATTGGCCCCGGGGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGAGGC‑TGGAAAACGAT‑CCGCAACCTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGt                                                                                              <  1:137998/237‑1 (MQ=255)

                                                                                                                                                 tCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGGTGATGCT‑CGACG‑CCCTCGGGGTGC‑TGGAAAACGAA‑CCGCAAAATG‑AAAAC‑ATTTCGCTTTTCTCCCAAACGCCTGCGCCTG‑CGGGGGCCCCCAAAAAGGTGGGACGTGCGCGCGCCCCCCCCAAGCCGGTGGTGGGCTGCTTTCCCGGTCGTggg                                                                                           >  2:124548/1‑238 (MQ=255)

                                                                                                                                                  ccGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑CACTGAGCGAGAAAATCGGCGGCCCGATGATGCT‑CGCCG‑CCCTCGGGGTGC‑TGGAAAAACAT‑CCGCAAACTG‑AAATC‑ATTGCGCCTTTCTCCAAACCGCCCGCGCCTG‑CGGGGGCCCCGCAAGTGCTGGAAACGGCGCGCCCCCGCCCCACGCCGGGGGGCGTCTGCCTCCCCTATCGTGGCg                                                                                          >  2:133214/1‑239 (MQ=255)

                                                                                                                                                      aTTCAGGAATTTGGCGGCGGCGTTGCGCAACTGATTGGCCCGGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAAc                                                                                      <  1:594437/239‑1 (MQ=255)

                                                                                                                                                        taaggAATTGGGGGGGGGGGTTTCGCAACGGATGGGCACCGGGGGGCGCG‑ACCTGAGGGAAAAAATCGGGGGCCTGAGGATGCG‑CGACG‑CCACCGGGAGGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATA‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGc                                                                                    <  2:133550/235‑1 (MQ=255)

                                                                                                                                                           tGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGCCG‑CCATCGGGGTGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCCGCCGCAAGCCGGTGGGCGTCTGCTTCCTCGATCGTGGCGAAACGCCAg                                                                                 >  1:68764/1‑239 (MQ=255)

                                                                                                                                                              aTTTGGCGGCGGCGTTTCGCAACTGATTGGCCCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTgg                                                                              <  1:927618/239‑1 (MQ=255)

                                                                                                                                                               tttGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTTAGCGAGAAAAACGGCGGCCTTATGGTGCT‑CGACG‑CCCTCGGGATGC‑TGGGAAAACAA‑CCGCCAAATG‑AAATC‑ATTTCGCTTATCTCCCAACCGCCCGCCCCTG‑CGGTGGCCCCCCAAATGCCGGGACGCGCGCGCGCCCGCCGCACCCCGGGGGGCGCTCGCTTCCTCGCTCGTGGGGAAAAGcccctggg                                                                             >  2:1399964/1‑233 (MQ=255)

                                                                                                                                                                  ggcggcggcGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGAGCGGGAAAAACGGCGGGCCGGTGAAGCT‑CCCCG‑CCCTCGGGGGGC‑TGGGAAACCAA‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAAACCCCTCCGCCTG‑CCGGGGGCCGCAAAGTGCTGGAACGGGCGCGCGCCCGCCCCCCACCGGGGGGGGTCCGCCTCCTCGATCGGGGCCAGACGCCAGTggcgga                                                                          >  2:791676/1‑235 (MQ=255)

                                                                                                                                                                  ggcggcggcGTTTCGCAACTGATTGGCACCGGCGGGCCCG‑CCCCCCGCGAGAAAATTTCCGGGCTGTTGATGCT‑CTACA‑CCCTCCGGTTTT‑CGGGAAACGAC‑CCGCCAACCGTAAATC‑CTT‑CGCTTATCTTCTCACCGCCCTCGCCCC‑CCGTGGCCCGCCAAGTGCTGGTAAGCGCGGGCCCCTGCCGCCACCCGGGCGTAGCCGTCGTCCTCGATCGGGGGGCAACGCCACTGGacgg                                                                          >  2:7937/1‑236 (MQ=255)

                                                                                                                                                                    cggcggcgTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAAACGGCGGCCTGATGGTGCT‑CGACG‑CCCTCGGGGTGC‑TGGGAAAAGAT‑CCGCAAAATG‑AAATC‑ATTGCGCTTATCTCCAAACCACCCGCGCCCG‑CGGGGGCCCGCAAAAGGCTGGGAAGTGCGCGCGCCTCCGCCACACCGGGGGGCGTCTGCTTCCTCCGTCGTGGGGGAAAGcccgagggtgggc                                                                        >  2:927618/1‑228 (MQ=255)

                                                                                                                                                                     ggtggggTTTAGCAACTGCTTGGCCCCGGCGGGCGCG‑ACCTGGACGAAAACACGGGCGACCTGTTGATGCT‑CGACC‑CCAGCGGGAGCC‑TGAAAAACGAT‑CCACAAACGG‑AAAAC‑TTGGGGCTTACTCCCAAACCGCCTGCGACTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGAGCGTGGCGAAACGCCAGTGGATGAGc                                                                        <  2:905454/232‑1 (MQ=255)

                                                                                                                                                                     gggggcgTTTCGCAACTGATGGGCCCCGGGGGGCGCG‑ACCTGAGGGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGAAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCCTTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                        <  2:1105895/235‑1 (MQ=255)

                                                                                                                                                                     ggcggctTTTCGCAACTGATTGGCACCGGCGGGCGGG‑ACCTGAGCGAAAAAACCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                        <  1:143225/238‑1 (MQ=255)

                                                                                                                                                                     ggcggcgTTGCGCAACTGATTGGCCCCGGCGGGCGCG‑CCCTGAGCGAGAAAACCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑GGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTTGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                        <  2:1322390/238‑1 (MQ=255)

                                                                                                                                                                     ggcggcgTTCCGCAACTGATTGGCACCGGGGGGCGCG‑CCCTGAGGGGGAAAACCGGCGGCCTGAGGATGCT‑CGACG‑CCATCGGGAGGC‑TGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                        <  1:250920/238‑1 (MQ=255)

                                                                                                                                                                      gcgtggTTCCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGAGGATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAC‑CCCCAAACGG‑AAATC‑ATTGCGTTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCa                                                                       <  1:13467/233‑1 (MQ=255)

                                                                                                                                                                           gTTTCGCAACGGAGTGGCACCGGCGGGCGCG‑ACCGGAGCGGAAAAATCGGCGGCCTGATGATGCC‑CGACG‑CCATGGGGATGC‑TGGAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGc                                                                  <  2:970918/238‑1 (MQ=255)

                                                                                                                                                                           gTTTCGCAACAGATGGGGCCCGGCGGGCGCG‑CCCTGAGAGGGAAAATCGGCGGCCTGAGGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGCT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGc                                                                  <  2:286373/238‑1 (MQ=255)

                                                                                                                                                                                       aTTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCAACGGGGTGC‑TGGAAAACGAA‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCCAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAAACGGCGCGCGCCTGCCGCAAGCCGGTGGGCGTCTGCTTCCTCCATCGTGGCGAAAAGCCAGTGGACGAGCAGGGGCTGAAGttttgcc                                                      >  2:205661/1‑234 (MQ=255)

                                                                                                                                                                                        gtGGCCCCGCCGGCCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑GGGAAACCGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCcg                                                     <  1:499269/237‑1 (MQ=255)

                                                                                                                                                                                         tGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCcgcg                                                   <  1:1246170/239‑1 (MQ=255)

                                                                                                                                                                                         gggCACCGGCGGGCGGG‑CCCTGAGCGAGAAAACCGGCGGCCTGATGATGCT‑CGGCG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACGG‑AAATC‑ATTGCGCTTATCTCCAAGCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCcgcg                                                   <  1:1048660/238‑1 (MQ=255)

                                                                                                                                                                                                 gcgggcgCG‑ACCTGGACGAGGAAATCCGCCGCCTGGTGATGCT‑CGACG‑CCCTCGGGACGC‑TGGGAAACGAT‑CCGCAAACTT‑AAATC‑CTTGCGCTTATCTCCACACCGCCTGCGCCTG‑CGGGGGGCCCCCAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCCGTGGGCGTCCGCCTCCTCGGGCGAGGCTGCACGCACGTGGGTGAGCAGGGGGCCCCGTTTGCCCGCGGCAccca                                            >  2:13530/1‑236 (MQ=255)

                                                                                                                                                                                                 gcgggcgCG‑ACCTGAGCGAGAAAATCGGCGGGCCGATGATGCT‑CGGAG‑CCATCGGGGTGC‑TGGAAAAAGCA‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGACTGCGCCTG‑CGGTGGCCCGCAAAATGCGGGAACGGGCGCGCGCCTGCCGCCAGCCGGCGGGCGGCTGCTTCCTCCATCGTGGCGGAACGCCAGTGGCGAGGCAGGGGGCTCCGATTGCCCCCGGCCCCaaa                                           >  2:280559/1‑239 (MQ=255)

                                                                                                                                                                                                      ggcg‑ACCTGAGCGAAAAAACGGGCGGCCGGATGATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAGCGAC‑CCGAAAACTG‑AAATC‑ATGCCGCTTATCTCCAAACCGCCTGCGCCGG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAgg                                       <  2:632567/237‑1 (MQ=255)

                                                                                                                                                                                                        gagacgtgggggaaaaaaTCGGCGGCTGGATGATCCC‑CGTCG‑CCGTCGGGATGG‑TGAAAAACGAT‑CCGAAAATGA‑AAATC‑AGTGGGTTTTTTCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGc                                      <  2:106200/224‑1 (MQ=255)

                                                                                                                                                                                                         g‑ACCTGGGCGAGAAAACCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                                   <  2:492227/239‑1 (MQ=255)

                                                                                                                                                                                                                ggcggaaaaaCCGGCGGCTTAATGATCCT‑CTACG‑CCACCGGTAGGC‑TGAAAACCGAC‑CCGCAAACTG‑AAATC‑ATTGCCCTTATCCCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGGGGGCTCGCCTGCCGCAAGCCGGGGGTCGTCTGCTTCCGCGGTCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGTCAGCGCTaa                              <  2:386321/232‑1 (MQ=255)

                                                                                                                                                                                                                      aaaaTCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATAAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGt                       >  2:321934/1‑239 (MQ=255)

                                                                                                                                                                                                                          tCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGAt                    >  2:720310/1‑238 (MQ=255)

                                                                                                                                                                                                                                gcgtgatgaTGCG‑GGTCG‑CCATCGGGATGC‑TGGAAACCGAC‑CCGCAAACTG‑AAACC‑ATTGCGTTTATCCCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCtctc              <  2:967740/235‑1 (MQ=255)

                                                                                                                                                                                                                                           cT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCCGCGCTAACAGCGGTGATGCTCTCCGGCGTGGAACa  >  2:750748/1‑239 (MQ=255)

                                                                                                                                                                                                                                           cT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACa  >  2:359675/1‑239 (MQ=255)

                                                                                                                                                                                                                                           cT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACa  >  1:45243/1‑239 (MQ=255)

GCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATGATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACA  >  NC_000913/337252‑337724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`