BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	337,515	T→C	18.5%	D246D (GAT→GAC)	yahF →	putative NAD(P)‑binding succinyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	337,515	0	T	C	18.5%	168.4 / 4.9	105	D246D (GAT→GAC)	yahF	putative NAD(P)‑binding succinyl‑CoA synthase
Reads supporting (aligned to +/- strand): ref base T (26/46); new base C (5/13); total (45/60)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACC  >  NC_000913/337282‑337753

ccAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGc                                                                                                                                                                                                                                               <  1:1082625/238‑1 (MQ=255)

 cAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCa                                                                                                                                                                                                                                              <  1:644394/238‑1 (MQ=255)

        tgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑gaa                                                                                                                                                                                                                                     >  1:1044309/1‑239 (MQ=255)

        tgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGGGGGAGAAAATCGGCGGGCTGATG‑CTGGT‑CGACG‑CCCTCGGGAAGC‑TGGGAAAAGAA‑CCCCAAACTG‑aaa                                                                                                                                                                                                                                     >  1:786740/1‑239 (MQ=255)

            aCGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑a                                                                                                                                                                                                                                 <  1:1214108/238‑1 (MQ=255)

            aCGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑a                                                                                                                                                                                                                                 <  1:515841/238‑1 (MQ=255)

              ggCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGGGAAAAATGGCGGCGTGGTG‑AGGTC‑CGCCG‑CCACCGGGGGGC‑TGGGAAACCAC‑ACGCAAACTG‑CAATC‑Ctt                                                                                                                                                                                                                               >  2:333814/1‑238 (MQ=255)

              ggCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTg                                                                                                                                                                                                                              <  2:1076777/239‑1 (MQ=255)

                cGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑GACTGGGCGGGGAAATCGGCGGGCCGGTG‑ATGGC‑CGAGG‑CCATACGGGTGG‑TGGGAAAACAT‑CCGCAAAcaggaaaccttgt                                                                                                                                                                                                                               >  2:326471/1‑226 (MQ=255)

                  aTTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGGGAGAAAATCGGCGGCCTGATG‑AAGGT‑CGGCG‑CCATCGGGGTGC‑TGGGAAACGAT‑CCGCAAACTG‑AAATC‑ATTTCGCt                                                                                                                                                                                                                          >  1:967740/1‑239 (MQ=255)

                  aTTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGCCG‑CCACCGGGGTGC‑TGGGAAACGAT‑CCCCCAACTG‑AAATC‑ATTTCGCt                                                                                                                                                                                                                          >  1:133550/1‑239 (MQ=255)

                   ttATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCaa                                                                                                                                                                                                                                             <  1:793321/221‑1 (MQ=255)

                   ttATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCt                                                                                                                                                                                                                          <  2:1158768/238‑1 (MQ=255)

                   ttATCAACGGCGCGGCGCTCTGTTGTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCt                                                                                                                                                                                                                          <  1:333814/238‑1 (MQ=255)

                            gcggggcgcTCTTTTTGGGTAACGCCGTGGGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCaa                                                                                                                                                                                                                 <  2:1080712/238‑1 (MQ=255)

                            gcgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGGC‑AACCGGGCGGGAAAATACGCGGGCCGAAT‑GTGCC‑CCGCGCCCACCGGGTTGG‑TGGAAAAGAAC‑CCCCAAACTG‑CAATC‑ATTGCCCtttcccccc                                                                                                                                                                                                                  >  2:549062/1‑231 (MQ=255)

                             tgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGTTATTGTTGCCGCATCCGGCGCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCaaa                                                                                                                                                                                                                <  1:543379/237‑1 (MQ=255)

                             cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGAG‑ACCTGAGGGAGGAAAACGGGGGCGCGAGG‑GTGCT‑TGACG‑CCCACGGGGAGG‑TGGGAAAAAAA‑CCGCCAACTG‑TAGAC‑ATTTCGCTCATTTCCaca                                                                                                                                                                                                                >  2:802727/1‑236 (MQ=255)

                             cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGGCTGATG‑ATGCT‑CGACG‑CCCTCGGGGTGC‑TGGGAAAAGAT‑CCGCAAACTG‑GAATC‑ATTTCGCTTATCTCCcaa                                                                                                                                                                                                                >  1:970918/1‑238 (MQ=255)

                                      cggTTTTGGTAACGCCGTGCGTCGCGGCAACAGCGGTATTGTGGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACcgcctgcgc                                                                                                                                                                                                      <  2:1323327/237‑1 (MQ=255)

                                           ttGGTAACGCCTTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCCCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAGACGGT‑CCGCACACTG‑AAATC‑CTTTCGCTTATCTCCAAACCGcctgcgcctg‑cg                                                                                                                                                                                                <  1:155260/239‑1 (MQ=255)

                                           ctGGGAACGGCGTGCGTCCCGGCACAATCTGTCTTGTTGGCGCACTCGGCCCCGGCGGTCAGGGGTTGAGCGTCCGCATTCGTGAATTGGGCGGCGGCGTTTCGCAACTGATGGCCTCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGAGG‑ATGCT‑CGACG‑CCAGCGGGATGC‑TGGAAAACGTT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGcctgcgcctg‑cg                                                                                                                                                                                                <  2:1370518/238‑1 (MQ=255)

                                            tGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGGGCGAGAAAAACCGCGGCCAGCTG‑GTGGT‑CGACG‑CCATCGGGGTGC‑AGGGAAAAGAG‑CCGCAAAACG‑AGAAA‑ATTTGGCTCTTCTCCAAACcgcccgctcccccgg                                                                                                                                                                                                >  2:964126/1‑234 (MQ=255)

                                                 aCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGGGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAAACGCCTTCGCCTG‑CGGTGGc                                                                                                                                                                                           >  1:399547/1‑238 (MQ=255)

                                                   gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCG‑ACCTGAGCGAGGAAATTGGCGGGCCGGTT‑ATGCT‑CGCCG‑CCCGCCGGGAGC‑TGGGAAAAGAT‑GCGCAAAACA‑TAATCAATTCCGCTTTTTTCC‑AACcgcctgccccctcggggggcc                                                                                                                                                                                          >  2:75138/1‑227 (MQ=255)

                                                   gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGAACCGGGGGGGGAAAAT‑GGGGGCCCGGTG‑ATGCC‑CGACG‑CCCCCGGGCTGCGGGGAAAACGAC‑CCGCAAACTA‑AAACA‑ATTGCCCTT‑TCTCCAAACcccccccccccgccgggggcc                                                                                                                                                                                          >  2:829232/1‑221 (MQ=255)

                                                   gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTTAGCGAGAAAAACCGCGGCCCGATG‑ATGCT‑CGGCG‑CCATCGGGGGGT‑TGGGAAACAAA‑ACGCAAACTG‑AAATC‑ATTCGGCCTTTCTCCACAAcgcctgccccgtagcggggcc                                                                                                                                                                                          >  2:913142/1‑227 (MQ=255)

                                                   gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCAGAGCGAGGAAATCGGCGGCCTCGTG‑ATGCG‑CGACA‑CCATCGGGGGGG‑TGGGAAACGGG‑CACCCAACTG‑AAATC‑GTCGCGCTTTTCTCCACACACCCCGCGCCTG‑CGCGGGccc                                                                                                                                                                                         >  2:689815/1‑238 (MQ=255)

                                                   gCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATCGGCGGGCTGGTG‑GTGCG‑CGGCG‑CCAACGGGGAGG‑CGGAAAACGAA‑CCCCAAACTG‑AAAAC‑CATGCGGTTTTCTCCCAAACCCCTGCCCCCG‑CGGGGGgcc                                                                                                                                                                                         >  2:586539/1‑238 (MQ=255)

                                                     gtggCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATGTGGCGGCGGCGTTTCGCAACGGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAGCTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  2:291417/236‑1 (MQ=255)

                                                     cgtggtttggggCAACATCGGAATTGTGTGCGCATCCGGCATCGGCAGTCAGGAGTGGAGCGTCCGCATTAATGAATTTGGCGGCGGCGTTTCCCAACGGATTGGCACCGGCGGGCGCG‑CCCTGAGCGAGAAAATCGGCGGCCGGATG‑AGGCG‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAAGC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  2:1115284/229‑1 (MQ=255)

                                                     cgtgcgtgGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCAGGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  2:1249863/239‑1 (MQ=255)

                                                     cggggggcgcgGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTGGAGCGTCCGCATTCATGAATTGGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  2:164330/232‑1 (MQ=255)

                                                     cGTGCGTCGGGGCAGCATCGGTATTGTTGGCGCATACGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAAGTGGGCGGCGGCGTTTCGCACCGGATGGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAAGCGGCGGCCGGATG‑GTGCG‑CGACG‑CCAGCGGGATGC‑TGGAAAACGAG‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCGCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  2:1162367/239‑1 (MQ=255)

                                                     cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑CCCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  1:995100/239‑1 (MQ=255)

                                                     cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  1:1399831/239‑1 (MQ=255)

                                                     cGTGCGGCGCGGCAACATCGGTATTGTGGGGGCATCCGGGACCGGCAGTCAGGAGTTGAGCGTCCGCATGCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACGG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCa                                                                                                                                                                                      <  2:1328212/239‑1 (MQ=255)

                                                       tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTTCGCTTATCTCCAAACCCCCCGCGCCCG‑CGGTGGGCCGCaca                                                                                                                                                                                    >  2:71906/1‑237 (MQ=255)

                                                       tGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATAGGCGGCCTGATG‑GTGGTCCGCCG‑CC‑TCGGGGGGC‑CGGGAAAACAC‑CCCCGAAATG‑CAATC‑ACTGCGCTTTTCTTCCCACCGCCTGCGCCTC‑GGGGGGccccgcaa                                                                                                                                                                                    >  2:701696/1‑234 (MQ=255)

                                                        gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCaaa                                                                                                                                                                                    <  1:862663/238‑1 (MQ=255)

                                                        gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCaaa                                                                                                                                                                                    <  1:75138/238‑1 (MQ=255)

                                                              cgGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑GACTTAGCGAGAAAAAAGGCGGGCCGGTG‑GTGGT‑CCGCG‑CCCACGGGGTGG‑TGGGAAAAGGT‑CCCCCAACTG‑AAATC‑CTTGCGCTTATCTCCAAAACCCCCTCGCCCG‑CGGGGGGCCCCCAAGGGCCgg                                                                                                                                                                             >  2:1399047/1‑239 (MQ=255)

                                                                      tCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTgcg                                                                                                                                                                     <  1:277845/239‑1 (MQ=255)

                                                                            tttttgGCGCATCCGGCGCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGcc                                                                                                                                                               <  1:1236747/236‑1 (MQ=255)

                                                                            ttgttgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGcc                                                                                                                                                               <  1:1240166/239‑1 (MQ=255)

                                                                               ttgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACACGGGCGCGAAAAACAGCGGCCTGGTG‑TTGCT‑CCGCC‑CCCTCCGGAGGG‑TGGAGAACGAT‑ACGCCAACTG‑AAATC‑ATTTCCGTTTTCTCCCAACCGCCCCCCCCTC‑CGCGGGGCCGCAAAggccctggccgaggcccccgccg                                                                                                                                                             >  2:250920/1‑216 (MQ=255)

                                                                                tgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCG‑ACCTGAGGGAGGAAATCGGCGGCCTGGTG‑GTTGG‑CGACG‑CCCCCGGGGTGC‑TGGGAAACGAA‑CCGCCAACTG‑AAATC‑ATTGAGCTTTTTTCCCAACAGACCGCGGCCG‑CGGGGGGCCGCCAAGTGGTGGAACGTGGGCGggccggcc                                                                                                                                                           >  2:872020/1‑239 (MQ=255)

                                                                                   cgcATCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAACC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGc                                                                                                                                                         <  1:71906/238‑1 (MQ=255)

                                                                                    gcATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGAGCGAGAAAAACGGGGGCCTGATG‑CTGCT‑CGACG‑CCCCCCGGGTGC‑TGGGAAAAGATACCCCAAAC‑G‑GAATC‑ATTGCGCCTATCTCCCCACCCCCCGCGCCTGCCGGGGGCCGCCAAAGGCCTGG‑ACGTgcccgccgcccgcgcaa                                                                                                                                                       >  2:208109/1‑228 (MQ=255)

                                                                                      accgggcacgggcaGTCGGGAGTTGAGCGTCCGAATTCATGAATTTGGGGGCGGCGTTTCGCAAGTGATGGGCACCGGCGGGCGGG‑CCCTGAGCGAAAAAAGCGGCGGCCGGATG‑ATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATGGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGc                                                                                                                                                     <  2:1293963/235‑1 (MQ=255)

                                                                                       tCCGGCCCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGTGCC‑GCCGCagaaca                                                                                                                                                   <  1:992012/239‑6 (MQ=255)

                                                                                       tCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCCGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGGTGC‑TGGAAAACGAT‑CCCCAAACTG‑AAATC‑ATTGCGCCTATCTCCAAACCGCCTGCGCCTG‑CGGGGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCCGCCGCCAGcc                                                                                                                                                    >  1:492227/1‑239 (MQ=255)

                                                                                         gggagcgggcaGCCAGGAGTTGGGGGTCCGCGTACATGAGTTGGGCGGCGGCTGTTGCCAACTGATTGCCCCCGGCGGGCTGG‑CCCTGAGCGAGAAAACCGGGGGCTTAATG‑AGGCT‑CGACC‑CCATCGGGATGC‑TGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCgg                                                                                                                                                  <  2:1021468/232‑1 (MQ=255)

                                                                                            caccggcaGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCCCG‑CCCTGAGCGAGAAAAACGGGGGCCCGATGTATCCC‑CG‑CC‑CCACCCGGGCGC‑TTGAAAAATAT‑CCACGAAATG‑GAATC‑ACTTCGCTTTTCTTCCAACCCCCTGCCCCTG‑CCGGGGGCCGCAAAAAGCTCGTACGAGCGCCCGCCTGCCGCCAGcgcgggg                                                                                                                                               >  2:1257342/1‑233 (MQ=255)

                                                                                              ccggcgGTCAGGAGTGGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTCCGCAACTGATTGGCCCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGgtc                                                                                                                                             <  1:802727/239‑1 (MQ=255)

                                                                                                                  gTCCTCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTgg                                                                                                                         <  1:1179859/239‑1 (MQ=255)

                                                                                                                  gTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCACCTGATTGGCCCCGGGGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGAGGC‑TGGAAAACGAT‑CCGCAACCTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGt                                                                                                                           <  1:137998/237‑1 (MQ=255)

                                                                                                                   tCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGGTG‑ATGCT‑CGACG‑CCCTCGGGGTGC‑TGGAAAACGAA‑CCGCAAAATG‑AAAAC‑ATTTCGCTTTTCTCCCAAACGCCTGCGCCTG‑CGGGGGCCCCCAAAAAGGTGGGACGTGCGCGCGCCCCCCCCAAGCCGGTGGTGGGCTGCTTTCCCGGTCGTggg                                                                                                                        >  2:124548/1‑238 (MQ=255)

                                                                                                                    ccGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGGG‑ACCTGAGCGAGGAAATCGGGGGCCTGATG‑ATGCT‑CGACG‑CCCTCGGGATGC‑TGGAAAAAGAA‑CCGCAAACTT‑AAATC‑AATGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGCACGTGCGCGCGCCTCGCGCAAGCCGGGGGGGGTATGCctcctccaagcgggcg                                                                                                                       >  2:820912/1‑229 (MQ=255)

                                                                                                                    ccGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑CACTGAGCGAGAAAATCGGCGGCCCGATG‑ATGCT‑CGCCG‑CCCTCGGGGTGC‑TGGAAAAACAT‑CCGCAAACTG‑AAATC‑ATTGCGCCTTTCTCCAAACCGCCCGCGCCTG‑CGGGGGCCCCGCAAGTGCTGGAAACGGCGCGCCCCCGCCCCACGCCGGGGGGCGTCTGCCTCCCCTATCGTGGCg                                                                                                                       >  2:133214/1‑239 (MQ=255)

                                                                                                                        aTTCAGGAATTTGGCGGCGGCGTTGCGCAACTGATTGGCCCGGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAAc                                                                                                                   <  1:594437/239‑1 (MQ=255)

                                                                                                                          taaggAATTGGGGGGGGGGGTTTCGCAACGGATGGGCACCGGGGGGCGCG‑ACCTGAGGGAAAAAATCGGGGGCCTGAGG‑ATGCG‑CGACG‑CCACCGGGAGGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATA‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGc                                                                                                                 <  2:133550/235‑1 (MQ=255)

                                                                                                                             tGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGCCG‑CCATCGGGGTGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCCGCCGCAAGCCGGTGGGCGTCTGCTTCCTCGATCGTGGCGAAACGCCAg                                                                                                              >  1:68764/1‑239 (MQ=255)

                                                                                                                                aTTTGGCGGCGGCGTTTCGCAACTGATTGGCCCCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTgg                                                                                                           <  1:927618/239‑1 (MQ=255)

                                                                                                                                    ggcggcggcGTTTCGCAACTGATTGGCACCGGCGGGCCCG‑CCCCCCGCGAGAAAATTTCCGGGCTGTTG‑ATGCT‑CTACA‑CCCTCCGGTTTT‑CGGGAAACGAC‑CCGCCAACCGTAAATC‑CTT‑CGCTTATCTTCTCACCGCCCTCGCCCC‑CCGTGGCCCGCCAAGTGCTGGTAAGCGCGGGCCCCTGCCGCCACCCGGGCGTAGCCGTCGTCCTCGATCGGGGGGCAACGCCACTGGacgg                                                                                                       >  2:7937/1‑236 (MQ=255)

                                                                                                                                      cggcggcgTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAAACGGCGGCCTGATG‑GTGCT‑CGACG‑CCCTCGGGGTGC‑TGGGAAAAGAT‑CCGCAAAATG‑AAATC‑ATTGCGCTTATCTCCAAACCACCCGCGCCCG‑CGGGGGCCCGCAAAAGGCTGGGAAGTGCGCGCGCCTCCGCCACACCGGGGGGCGTCTGCTTCCTCCGTCGTGGGGGAAAGcccgagggtgggc                                                                                                     >  2:927618/1‑228 (MQ=255)

                                                                                                                                       ggtggggTTTAGCAACTGCTTGGCCCCGGCGGGCGCG‑ACCTGGACGAAAACACGGGCGACCTGTTG‑ATGCT‑CGACC‑CCAGCGGGAGCC‑TGAAAAACGAT‑CCACAAACGG‑AAAAC‑TTGGGGCTTACTCCCAAACCGCCTGCGACTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGAGCGTGGCGAAACGCCAGTGGATGAGc                                                                                                     <  2:905454/232‑1 (MQ=255)

                                                                                                                                       gggggcgTTTCGCAACTGATGGGCCCCGGGGGGCGCG‑ACCTGAGGGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGAAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCCTTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                     <  2:1105895/235‑1 (MQ=255)

                                                                                                                                       ggcggctTTTCGCAACTGATTGGCACCGGCGGGCGGG‑ACCTGAGCGAAAAAACCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                     <  1:143225/238‑1 (MQ=255)

                                                                                                                                       ggcggcgTTGCGCAACTGATTGGCCCCGGCGGGCGCG‑CCCTGAGCGAGAAAACCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑GGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTTGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                     <  2:1322390/238‑1 (MQ=255)

                                                                                                                                       ggcggcgTTCCGCAACTGATTGGCACCGGGGGGCGCG‑CCCTGAGGGGGAAAACCGGCGGCCTGAGG‑ATGCT‑CGACG‑CCATCGGGAGGC‑TGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc                                                                                                     <  1:250920/238‑1 (MQ=255)

                                                                                                                                        gcgtggTTCCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGAGG‑ATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAACGAC‑CCCCAAACGG‑AAATC‑ATTGCGTTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCa                                                                                                    <  1:13467/233‑1 (MQ=255)

                                                                                                                                             gTTTCGCAACTGATGGGCACCGGCGGGCGCG‑CCCGGAGCGAGAAAATCGGCGGCCTGATG‑ATGCC‑CGACG‑CCATCGGGATGC‑GGGAAAACGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGc                                                                                               <  1:829232/238‑1 (MQ=255)

                                                                                                                                             gTTTCGCAACGGAGTGGCACCGGCGGGCGCG‑ACCGGAGCGGAAAAATCGGCGGCCTGATG‑ATGCC‑CGACG‑CCATGGGGATGC‑TGGAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGc                                                                                               <  2:970918/238‑1 (MQ=255)

                                                                                                                                             gTTTCGCAACAGATGGGGCCCGGCGGGCGCG‑CCCTGAGAGGGAAAATCGGCGGCCTGAGG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGCT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGc                                                                                               <  2:286373/238‑1 (MQ=255)

                                                                                                                                                         aTTGGCACCGGCGGGCGCG‑AACTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCAACGGGGTGC‑TGGAAAACGAA‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCCAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAAACGGCGCGCGCCTGCCGCAAGCCGGTGGGCGTCTGCTTCCTCCATCGTGGCGAAAAGCCAGTGGACGAGCAGGGGCTGAAGttttgcc                                                                                   >  2:205661/1‑234 (MQ=255)

                                                                                                                                                          gtGGCCCCGCCGGCCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑GGGAAACCGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCcg                                                                                  <  1:499269/237‑1 (MQ=255)

                                                                                                                                                           tGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCcgcg                                                                                <  1:1246170/239‑1 (MQ=255)

                                                                                                                                                           gggCACCGGCGGGCGGG‑CCCTGAGCGAGAAAACCGGCGGCCTGATG‑ATGCT‑CGGCG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACGG‑AAATC‑ATTGCGCTTATCTCCAAGCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCcgcg                                                                                <  1:1048660/238‑1 (MQ=255)

                                                                                                                                                                   gcgggcgCG‑ACCTGGACGAGGAAATCCGCCGCCTGGTG‑ATGCT‑CGACG‑CCCTCGGGACGC‑TGGGAAACGAT‑CCGCAAACTT‑AAATC‑CTTGCGCTTATCTCCACACCGCCTGCGCCTG‑CGGGGGGCCCCCAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCCGTGGGCGTCCGCCTCCTCGGGCGAGGCTGCACGCACGTGGGTGAGCAGGGGGCCCCGTTTGCCCGCGGCAccca                                                                         >  2:13530/1‑236 (MQ=255)

                                                                                                                                                                   gcgggcgCG‑ACCTGAGCGAGAAAATCGGCGGGCCGATG‑ATGCT‑CGGAG‑CCATCGGGGTGC‑TGGAAAAAGCA‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGACTGCGCCTG‑CGGTGGCCCGCAAAATGCGGGAACGGGCGCGCGCCTGCCGCCAGCCGGCGGGCGGCTGCTTCCTCCATCGTGGCGGAACGCCAGTGGCGAGGCAGGGGGCTCCGATTGCCCCCGGCCCCaaa                                                                        >  2:280559/1‑239 (MQ=255)

                                                                                                                                                                       gcgcG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCC‑CCCCG‑CCCTCGGGGTGC‑TGGGAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGGGGCCCGCAAAGTGCCGGAACGTGCGCGCGCCTGCCGCCAGCCGGGGGGCGGCTGCTTCCTCGGTCGTGGCGAAACGCCCGTGGATGGGCAGGGGCCACAGTTTTCCCGCGGCCCCCAAGAgg                                                                    >  2:640608/1‑239 (MQ=255)

                                                                                                                                                                        ggcg‑ACCTGAGCGAAAAAACGGGCGGCCGGATG‑ATGCC‑CGACG‑CCATCGGGATGC‑TGGAAAGCGAC‑CCGAAAACTG‑AAATC‑ATGCCGCTTATCTCCAAACCGCCTGCGCCGG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAgg                                                                    <  2:632567/237‑1 (MQ=255)

                                                                                                                                                                          gagacgtgggggaaaaaaTCGGCGGCTGGATG‑ATCCC‑CGTCG‑CCGTCGGGATGG‑TGAAAAACGAT‑CCGAAAATGA‑AAATC‑AGTGGGTTTTTTCCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGc                                                                   <  2:106200/224‑1 (MQ=255)

                                                                                                                                                                           g‑ACCTGGGCGAGAAAACCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc                                                                <  2:492227/239‑1 (MQ=255)

                                                                                                                                                                                        aaaaTCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATAAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGt                                                    >  2:321934/1‑239 (MQ=255)

                                                                                                                                                                                            tCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGAt                                                 >  2:720310/1‑238 (MQ=255)

                                                                                                                                                                                                  gcgtgatg‑aTGCG‑GGTCG‑CCATCGGGATGC‑TGGAAACCGAC‑CCGCAAACTG‑AAACC‑ATTGCGTTTATCCCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCtctc                                           <  2:967740/235‑1 (MQ=255)

                                                                                                                                                                                                         gatgtggccggaccccttggggATGC‑TGAAAAACGTC‑CCGCAACCAG‑AAACC‑TTTGCCTTTTTCCCCAACCCGCCTGCGCCTG‑CGGGGGCCCGCAAAGGGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCg                                      <  2:844804/220‑1 (MQ=255)

                                                                                                                                                                                                            atcctcagcG‑CCGTCGGGATGC‑TGGAAAACGAC‑CCGCAAACTG‑AAACC‑ATGGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGa                                   <  1:913142/230‑1 (MQ=255)

                                                                                                                                                                                                              cT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCCGCGCTAACAGCGGTGATGCTCTCCGGCGTGGAACa                               >  2:750748/1‑239 (MQ=255)

                                                                                                                                                                                                              cT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACa                               >  2:359675/1‑239 (MQ=255)

                                                                                                                                                                                                              cT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACa                               >  1:45243/1‑239 (MQ=255)

                                                                                                                                                                                                                        cATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGaaaa                         >  1:395252/1‑237 (MQ=255)

                                                                                                                                                                                                                         aTCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAAtc                       >  2:199356/1‑238 (MQ=255)

                                                                                                                                                                                                                          tCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAAtctc                     >  2:690195/1‑239 (MQ=255)

                                                                                                                                                                                                                              gATGC‑AGGAAACCGAC‑CCGCAAACTG‑AAATC‑ATTGCGCTTACCTCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCCGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGAc                  <  1:640608/238‑1 (MQ=255)

                                                                                                                                                                                                                               gagccgggAAACCGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTACCTCCAACCCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGAc                  <  2:582851/232‑1 (MQ=255)

                                                                                                                                                                                                                                              t‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAATTCTCGACCTGCATACGCTTAAc   >  2:1251126/1‑238 (MQ=255)

                                                                                                                                                                                                                                              t‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAAcc  >  1:1230180/1‑239 (MQ=255)

CCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCG‑ACCTGAGCGAGAAAATCGGCGGCCTGATG‑ATGCT‑CGACG‑CCATCGGGATGC‑TGGAAAACGAT‑CCGCAAACTG‑AAATC‑ATTGCGCTTATCTCCAAACCGCCTGCGCCTG‑CGGTGGCCCGCAAAGTGCTGGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGCGCTAAAAGCGGTGATGCTCTCCGGCGTGAAACAGGAAAATCTCGACCTGCATACGCTTAACC  >  NC_000913/337282‑337753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`