BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,782,889	A→C	13.9%	S258A (TCG→GCG)	gpsA ←	glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,782,889	0	A	C	13.9%	209.9 / 4.3	110	S258A (TCG→GCG)	gpsA	glycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand): ref base A (29/58); new base C (11/7); total (45/65)
Fisher's exact test for biased strand distribution p-value = 3.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑GAAATCCGGATTGCTGTAAAC  >  NC_000913/3782657‑3783126

tCGTCCTTTCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGtt                                                                                                                                                                                                                                                <  1:290924/239‑1 (MQ=255)

tCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGtt                                                                                                                                                                                                                                                <  1:635069/239‑1 (MQ=255)

tCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGtt                                                                                                                                                                                                                                                <  2:1008642/239‑1 (MQ=255)

  gTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCGCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGt                                                                                                                                                                                                                                              <  1:606145/239‑1 (MQ=255)

  gTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGt                                                                                                                                                                                                                                              <  1:1065833/239‑1 (MQ=255)

  gTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGt                                                                                                                                                                                                                                              <  2:550097/239‑1 (MQ=255)

  gTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGt                                                                                                                                                                                                                                              <  2:1340811/239‑1 (MQ=255)

  gTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGt                                                                                                                                                                                                                                              <  2:1196759/239‑1 (MQ=255)

       tGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTa                                                                                                                                                                                                                                         <  1:880942/239‑1 (MQ=255)

       tGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTa                                                                                                                                                                                                                                         <  2:1055122/239‑1 (MQ=255)

        gCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTAc                                                                                                                                                                                                                                        <  2:863483/239‑1 (MQ=255)

        gCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTAc                                                                                                                                                                                                                                        <  2:1135115/239‑1 (MQ=255)

               cGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAg                                                                                                                                                                                                                                 <  2:1276830/239‑1 (MQ=255)

                            tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAg                                                                                                                                                                                                                           >  1:137618/1‑232 (MQ=255)

                            tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAg                                                                                                                                                                                                                           <  2:137618/232‑1 (MQ=255)

                            tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGAGACTGGTTGTCGGTACAGGTAAGCACCAGAACGccc                                                                                                                                                                                                                    >  2:963021/1‑238 (MQ=255)

                            tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACCCGCACGGTTTTCTGGCCAGGTAAGcccccaataccc                                                                                                                                                                                                                     >  2:334791/1‑230 (MQ=255)

                            tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACCCCCCTGGGTGTCGGTACAGGTCAGCCCCacaacaccc                                                                                                                                                                                                                    >  2:954834/1‑231 (MQ=255)

                            tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑GTACCCGGCCGGTTGTCGGTACCGGTAAGCACCAAAACGccc                                                                                                                                                                                                                    >  2:979578/1‑238 (MQ=255)

                                tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGG‑TTACGCGACTGGTTGTTGGTACAGGTAAGCACCAAATACCC‑aacc                                                                                                                                                                                                                >  1:1284619/1‑236 (MQ=255)

                                tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGGTTTCGGTACAGGGAAGGCCCAAAACGCC‑CAGc                                                                                                                                                                                                                >  2:424139/1‑238 (MQ=255)

                                     ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTAAGCCACTGGTTGTCGGTGCCGGGAAGCACCAGAACGCC‑AAGCCCCGc                                                                                                                                                                                                           >  1:118653/1‑238 (MQ=255)

                                      cTCGCGCTCGTTTTTTCCGCAATATAATACTTGATAATGTTCCTCTGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTCGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACGGGTAAGCACCAGATCGCA‑AAGCCCCGCCa                                                                                                                                                                                                         <  2:34697/239‑1 (MQ=255)

                                       ccgcgcgcgTTTTTTCCGAAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAGGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCAt                                                                                                                                                                                                        <  2:1253600/238‑1 (MQ=255)

                                                          aaTATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATa                                                                                                                                                                                                 <  1:1016856/227‑1 (MQ=255)

                                                            tataATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCCCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAAACTTCTCCGGCGCTCTTTGTACATCCATGCCCTGACCGAGCATCAGGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCAt                                                                                                                                                                                                        <  1:1389937/218‑1 (MQ=255)

                                                                 taCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACCCGCCTGGGTGTCGGGGCAGGTAAGCACCAAAACGCCAAACCCCCGCCA‑GCCCCCCAAGGGGGCAGGggcggcc                                                                                                                                                                               >  2:1152983/1‑237 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCATCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTACACACCGGGTTTTGGGCAC‑GGTAAGCACCAAATCCCC‑AAACCCCCCCCCGCCCATAAAAAGGGCAgggggagcgcccca                                                                                                                                                                          >  2:248159/1‑227 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCTTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGCCTGGGTGTCGGGACAGGTAAGCACCCAAACCCC‑CAACCCCCCCCCGCCCACAAAAGTGGCAGGGGCGGCAccccc                                                                                                                                                                          >  2:1204478/1‑236 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTACAC‑CCTGGTTTTCTGTACAAGTAAGCACCAAAAAGGC‑CAACCCCCCCACACCCATaaaagctgtctctttaacacaactc                                                                                                                                                                         >  2:1389937/1‑217 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCTCCTGGTTGTCGGTACAGGGAAGAACCAGATCCCC‑AAAACCCCCCCCGCCCCTAAAAGGGGCAGGGTCGGGACCCac                                                                                                                                                                          >  1:39806/1‑237 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGCATGGTTGTCGGGACAGGGAAACACCAGAACCCC‑AAACCCCGCCATGCCCATAAAGGTGGCAGGGGCGGCAccccc                                                                                                                                                                          >  1:1135680/1‑236 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTGCAGGTAAGCACCAAAAAGCC‑AAGCCCCGCCCTGCCCCTAAAAGGGGGAGGGGCGGGAccccg                                                                                                                                                                          >  2:487356/1‑236 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGGACAGGTAAGCACCAAATCGCC‑AAACCCCGCCATGCCCATAAAGGTGGCAGGGGCGGCACCCAg                                                                                                                                                                          >  1:224797/1‑238 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGGACAGGGAAACAACAAAACGCC‑AAACCCCCCCCATCCCCTAAAGGTGGCCAGGGCGGCAACCac                                                                                                                                                                          >  1:1409045/1‑237 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGGTTTCGGGACAGGGAAGCACCAAAACCCC‑CAACCCCCCCCTTCCCCTAAAGGGGGCAGGGGCgggcccccc                                                                                                                                                                          >  2:769259/1‑231 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACCGGTTTTCGGTACAGGTAAGCACCAGATCGCC‑AAACCCCCCCATGCCCCTAAAAGTGGCAGGGTCGGCACCCAg                                                                                                                                                                          >  1:203874/1‑238 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCCGCCGGTTTTCGGTACAGGTAAGCACCAGAACGCC‑CACCCCCGCCCTGCCCCTAAAGGTGGCAGGGGCGGCACCCCgc                                                                                                                                                                         >  2:378119/1‑239 (MQ=255)

                                                                      gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTAAGCGCCCGGTTGTCGGTACAAGTAAAACCCACAACCCC‑CAACCCCCCCCTTCCCCTAAAGAGGGCCGGGGCGGCAccccg                                                                                                                                                                          >  2:1067617/1‑236 (MQ=255)

                                                                        tAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTTCGCGCCTGGTTGTCGGTACAGGGAAGCACCAAATCCCC‑AAGCCCCGCCCTTCCCATAAAGGGGGCAGGGTCGGCACCCAgcc                                                                                                                                                                        >  1:932088/1‑237 (MQ=255)

                                                                                  cGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGa                                                                                                                                                             <  1:938984/239‑1 (MQ=255)

                                                                                     ttATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCACCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑cg                                                                                                                                                          <  1:419490/238‑1 (MQ=255)

                                                                                      tATTGGCATTTCAACGCCGGGGCGTTGCGCCGTTTCGCGTTCTTTTTTCGTATTGCGGTAGCCTCCCACCCCCGGGCCAATCTTCTCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑cgcg                                                                                                                                                        <  2:966754/239‑1 (MQ=255)

                                                                                           gCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTa                                                                                                                                                                                                                                         <  1:348497/155‑1 (MQ=255)

                                                                                               ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGAc                                                                                                                                                                                                                                                     >  2:1307441/1‑139 (MQ=255)

                                                                                               ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGGAAGCACCAAATCGCC‑CAGCCCCCCCATGCCCCTAAAAGTGGCAGGGGCGGCACCCAGCCCCGCACCAAAA‑CGCGACATTTc‑a                                                                                                                                               >  2:581323/1‑238 (MQ=255)

                                                                                               ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTCTCGGTACAGGGAAGCACCAGATCGCC‑CAACCCCGCCATGCCCATAAAGGTGGCAGGGTCCGCACCCAGCGCCCCACCaaacaccgacaattct                                                                                                                                                 >  1:1330029/1‑224 (MQ=255)

                                                                                               ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGGTGTCGGGACAGGTAAGCACCAAAACGGC‑AAGCCCCCCCAAGCCCCTAAAGGGGGCAGGGGCGGCACCCCGCGCCCCACCCAGA‑CGCGACAATTc‑a                                                                                                                                               >  1:1217021/1‑238 (MQ=255)

                                                                                               ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCCACCGGTTGTCGGGACAAGGAAGCACCAAATCCCC‑CAGCCCCCCCCTGCCCCTAAAAGTGGCCGGGTCGGCACCCAGCGCCGCCCCAAGA‑CGCGACATTTcc                                                                                                                                                >  2:725121/1‑237 (MQ=255)

                                                                                                tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTc‑agc                                                                                                                                             <  1:424139/239‑1 (MQ=255)

                                                                                                gcAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTc‑agc                                                                                                                                             <  1:487356/238‑1 (MQ=255)

                                                                                                caaaCGCGGAAGCGATGCGCCAGTTCGCGGCCTTTTTTCGTATTGCGGTCGCCTTCCACCCCCTGACAAATCTTCCCCTGCGCGCTTTGTACTTCCATGCCCGGACCGAGCATCATTCCAAAACGGCGG‑TTACGCGCCTGGTTGTCGGTAAAGGTAAGCACCAGATCGCC‑AGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTc‑agc                                                                                                                                             <  2:1015847/237‑1 (MQ=255)

                                                                                                       cGAGGCGATGCGCCAGTCCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCa                                                                                                                                      <  1:581323/239‑1 (MQ=255)

                                                                                                        gAAGCGATGCGCCCGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCCCCTGCGCGTTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCAc                                                                                                                                     <  2:932088/239‑1 (MQ=255)

                                                                                                         aaGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACCCGACTGGGTTTCGGGACAGGCAAGGACCCGACCGCC‑AAGCCCCGCCCTGCCCCTacaa                                                                                                                                                                                              >  2:85238/1‑180 (MQ=255)

                                                                                                          aGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACgg                                                                                                                                   <  1:63073/239‑1 (MQ=255)

                                                                                                                  gcCAGTCCGCGGTCTTTTTTCGTATTGCGGTTGCTTCCCCCCACCGTACCAACCTTCTCCGCGGCGCTTTGTACAGCCATGCCCTGACCGAGCATCATGCAAAAACGGCGG‑TTACGCGCGTGGTTGTCGGTACAGGAAAGCACCAGACCGCC‑AGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAg                                                                                                                           <  2:1217021/239‑1 (MQ=255)

                                                                                                                    cagtcgccgGTCTTTTTTCGTATGGCGGTACCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGTTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGTTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAgcg                                                                                                                         <  2:451949/232‑1 (MQ=255)

                                                                                                                         cgcgGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGGTGTCGGTACAGGTAAGCACCAGATCGCC‑AAACCCCGCCACGCCCCTAAAGGCGGCAGGGGCGGCACCCCCCGCCGCCCCCAGA‑CCCCACATTTC‑AGCCAGCCCACGCGTGGTCAGCGCCgc                                                                                                                     >  2:463937/1‑237 (MQ=255)

                                                                                                                                       gTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc                                                                                                      <  1:248159/239‑1 (MQ=255)

                                                                                                                                        tATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACCGGGTTTCGGGTCAGGGAAGGCCCAGAACGCC‑CAAACCCCCCACGCCCCTAAAAGGGGGAGGGGCGGCACCCCGCCCCGACCCCCAA‑CCCGGAATTTT‑AGCCACGCCACAGGGGGTCAGCGcccccagcgccctcgcccca                                                                                                     >  2:910755/1‑221 (MQ=255)

                                                                                                                                          ttGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGGCTTGTTGTCGGGACAGGTAAGCACCAGAACGCC‑AAGCCCCGCCCTGCCCATAAAAGTGGCAGGGGCGGCCCCCAGCGCCGCCCCAAGACCGCG‑CATTTC‑AGCCCGCCCCCCGGGGCACAGCGCCGTACGCGCGTTCGCCCCaca                                                                                                   >  2:464782/1‑237 (MQ=255)

                                                                                                                                          ttGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCaaa                                                                                                   <  1:102266/239‑1 (MQ=255)

                                                                                                                                                tacccTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                              <  1:769259/235‑1 (MQ=255)

                                                                                                                                                tAGCCTTCCCCCACCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGG‑TTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                              <  1:988602/238‑1 (MQ=255)

                                                                                                                                                         cccaccTGACAAATCTCCTCCTGCGCGCTTGGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑a                                                                                   <  1:378119/238‑1 (MQ=255)

                                                                                                                                                         ccacccTGACAAATCTTCTCCTGCGCGCTTTGTCCACCCATGCCCGGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGCCGGTACAGGTAGGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑a                                                                                   <  1:774673/235‑1 (MQ=255)

                                                                                                                                                         acccccGGACCAATCTTCTCCTGCGGGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGACCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑a                                                                                   <  1:421624/239‑1 (MQ=255)

                                                                                                                                                         accaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCAAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑a                                                                                   <  1:1204478/239‑1 (MQ=255)

                                                                                                                                                          ccaccTGACAAATCTTCCCCTGCGCGCTTTGTACACCCATGCCCTGCCCGAGCAGCATGCCAAACCGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑Ac                                                                                  <  2:203874/239‑1 (MQ=255)

                                                                                                                                                               tgcccAATCTTCCCCTGCGCGCTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATccc                                                                             <  1:939665/236‑1 (MQ=255)

                                                                                                                                                               tgcccAATCTCCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGCCTGGTTGCCGGTACAGGTAAGCCCCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATccc                                                                             <  1:963021/236‑1 (MQ=255)

                                                                                                                                                                caCCAATCTTCTCCGCCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGCCTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATcccc                                                                            <  2:224797/238‑1 (MQ=255)

                                                                                                                                                                          ccccTGCGCGCTTTGTACATCCATGCCCTGACGGAGCATCAGGCCAAACCGGCGG‑TTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATgg                                                                 <  1:725121/237‑1 (MQ=255)

                                                                                                                                                                                cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGGCTGGTTGTGGGGTAAGGTAAAGCCCAGATAGCC‑AAACCCCCCCCTGCCCATAAAAGTGGGAGGGTAGGCCCCCCCCCCCGCCCCCAGA‑AGCGGCAATTT‑AGCCCCCCCACGCGTGAGCAGCCCCCGACCCCCTTTCCGACCCCAACCGCTACCACCGG‑CCACACCCGCACC‑AACGGCACTa                                                            >  2:910190/1‑238 (MQ=255)

                                                                                                                                                                                             tCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTTACCCACCGGTTGTCGGTACAGGGAAGCACCAAAACGCC‑CAGCCCCGCCCCCCCCCTAAAGGCGGCAGGGGCGGCACCCCCCGCCGCCCCACAA‑AGCGCCACTTCAACCCACCCCACGGGGGA‑CAGCGCCCCACCCGCACTCGCCCCAAACACCATAACGTCCG‑CCACCCCCCCCAC‑CACCGCAAAA‑AACTTTTTCAccc                                              >  2:251512/1‑237 (MQ=255)

                                                                                                                                                                                              ccAGGCCCTGACCGAGCATCAGGCCAAAACGGCGG‑TTACGCGACTGGTTGCCGGTCCAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATGCCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACcgcg                                            <  1:646801/239‑1 (MQ=255)

                                                                                                                                                                                                 tGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTa                                                                                                                                                                                                                                         >  2:943808/1‑53 (MQ=255)

                                                                                                                                                                                                 tGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTa                                                                                                                                                                                                                                         <  1:943808/53‑1 (MQ=255)

                                                                                                                                                                                                 tGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAAAACGCC‑CAGCCCCGCCATGCCCATACAGGCGGCAGGGGCGGCACCCAGCGCCGCCCCCAGA‑CGCGGCATTTC‑AGCCAGCCCACGGGGGGTCAGCGCCGCACGCGCATTCGCCCCAAAACCGATACCGCCGG‑ACAACCCCCCCCC‑CACACCAATA‑ACGATTTTCACCGcgcc                                          >  2:1386684/1‑238 (MQ=255)

                                                                                                                                                                                                 tGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACCCGACTGGGTGTCGGGAAAGGTAAACACCAAAACGCC‑AAACCCCGCCCTTCCCCAAAAAGGGGGAGGGGCGGGACCCAGCGCCGCACCCCGA‑CGCCACAATTC‑AGCCAGCCCACGGGGGGTCAGCGCCGTACCTGCCTTCTCACCAAAAACCATACCCTCGG‑ACAACCCCCCACC‑ACTGGCACTa‑aaactttttaacgcccc                                          >  2:666231/1‑222 (MQ=255)

                                                                                                                                                                                                 gcccacacccGGGCATCGTGCCAAACCGGCGG‑TTCCGCGACTGGTTGTCGGTACAGGTAACCACCAAAGCGCC‑GAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGcgccg                                         <  2:654967/231‑1 (MQ=255)

                                                                                                                                                                                                  gCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAgcgc                                                                                                                                                                       <  2:1098848/117‑1 (MQ=255)

                                                                                                                                                                                                  ccccTGCCCGACCATCATCCAAAACCGGCGT‑TTCCGCGACTGTGTGGCGGTACGGCTAAGCACCAGATCCCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGcgccg                                         <  2:1284619/237‑1 (MQ=255)

                                                                                                                                                                                                        aCCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTTTCGGGACAGGGAAACAACAAATCGCC‑AAGCCCCCCCAAGCCCCTAAAAGGGGGAGGGGCGGCACCCCCCGCCGCACCCAGA‑CCCGCCAATTC‑AGCCAGCCCACCGGGGATCAGCGCCGCACGCCCACTCCCACCCAAACCGACACCCTCGG‑ACAACCCCCCACC‑ACAGGCAATA‑ACGATTTTCACCGCGCCGCCGAGc                                   >  2:776819/1‑238 (MQ=255)

                                                                                                                                                                                                        aCCGAGCATCATGCCAAAACGGCGG‑GTACGCGACTGGTTGTCGGTACAGGTAAGCACCAAATCGCC‑AAACCCCCCCCAGCCCCAAAAAGAGGCAGGGGCGGACCCCCGCGCCCCCCCAAGA‑CGCGACAATTT‑ACCCAGCCCACCGGGGAGCCGCCCCCTACACCCCCTCCCACCCAAACCGATAACGGCGGCACA‑CCCCGCACCAAACGGCAACACACGTTTTCCCCCGccccccaac                                     >  2:696631/1‑233 (MQ=255)

                                                                                                                                                                                                         ccgaccagagtGCAAAAAGGGCGG‑TTACGCGACTGGTGGCGGGAACAGGAAACCACCAGACCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTg                                 <  2:39806/229‑1 (MQ=255)

                                                                                                                                                                                                         ccGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTg                                 <  1:979578/239‑1 (MQ=255)

                                                                                                                                                                                                         ccGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGCCGGTACAGGTAAGCCCCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTg                                 <  1:1152983/239‑1 (MQ=255)

                                                                                                                                                                                                         ccGACCATCATGCAAAAACGGCGG‑TAACGCGACGGTTTGTCGGTAAAGGTAAGCACCAGACCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTg                                 <  1:910190/239‑1 (MQ=255)

                                                                                                                                                                                                            aGCATCATGCCAAAACGGCGG‑TTACGCGACTGGGTGTCGGGACAGGGAAGCACCAGATCGCC‑AAACCCCGCCATGCCCATAAAGGGGGCAGGGGCGGCACCCAGCGCCGCAcccaaa                                                                                                                                                             >  1:1098848/1‑113 (MQ=255)

                                                                                                                                                                                                               atcagGCCAAACCGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGc                            <  1:954834/238‑1 (MQ=255)

                                                                                                                                                                                                                  atGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGGACACGTAAGCAACAAAACCCC‑AAACCCCGCCATGCCCATAAAAGTGGGAGGGGTGGCACCCAGCGCCGCCCCAAAA‑ACCGACATTTT‑AGCCCCCCCCCCGGGGAAAAGCGCCGCACCCGTCTTCGCACCAAAACCGGTACCGTCCG‑ACATCCCCCCCCC‑AACGGCCATA‑ACGGTTTTCACCCCcccgcccacactcaccccaac                        >  2:799458/1‑224 (MQ=255)

                                                                                                                                                                                                                   tGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGGACAGGGAAGCACCAAATCGCC‑CAGCCCCGCCATGCCCATAAAGGGGGCAGGGGCGGCACCCCGCACCGCACCAAAA‑CGCGACATTTC‑AACCAGCCCCCGGGGGATCAGCGCCGCACGCGCATTCCCACCAAAACCGATACCGGCGG‑ACAACCCCCCACC‑CACGGACAAA‑ACGCTTTTTACCCCgccgccccactccacccccaag                       >  2:956506/1‑223 (MQ=255)

                                                                                                                                                                                                                       aaacCGGCGG‑TTCCGCGCCTGGTTGCCGGTACAGGTAAGCCCCAGCTCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑GAAAt                  <  1:955100/239‑1 (MQ=255)

                                                                                                                                                                                                                             gcgg‑TTACGCGACTGGTCGTCGGTACAGGTAAGCACCAGACCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑GAAATCCGGa             <  1:696631/238‑1 (MQ=255)

                                                                                                                                                                                                                              ggg‑TTACGCCCCTGGTTGTCGGTACAGGTAAGCACCAGACCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑GAAATCCGGAt            <  1:1386684/237‑1 (MQ=255)

                                                                                                                                                                                                                              cgg‑TTACGCGACTGGTTGTCGGGACAGGGAAGCACCAAAACGCC‑AAGCCCCGCCATGCCCATAAAGGGGGCCGGGGCGGCACCCAGCGCCGCCCCCAAA‑CGCCACATTTC‑AGCCAGCCCACGGGGGATCAGCGCCGGACGCGCATTCGCACCAAAAACGAAACCGCCGG‑ACATCCCCGCACC‑AAAGGCAATA‑ACGTTTTTCACCCCCCCCCCCAACCGCACGCCAATCGAAACCGGGAt            >  2:342987/1‑239 (MQ=255)

                                                                                                                                                                                                                                     cgcgACTGGTTGGCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑GAAATCCGGATTGCTGTa     <  1:334791/239‑1 (MQ=255)

                                                                                                                                                                                                                                         aCTGGTTGTCGGTACAGGTAAGCACCAGACCCCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑Gatctccagcagctgctgcac  <  1:463937/238‑20 (MQ=255)

TCGTCCTTGCGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGG‑TTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCC‑AAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGA‑CGCGACATTTC‑AGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGG‑ACATCCCCGCACC‑AATGGCAATA‑ACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAAT‑GAAATCCGGATTGCTGTAAAC  >  NC_000913/3782657‑3783126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`