BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	353,606	A→G	15.2%	M301V (ATG→GTG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,606	0	A	G	15.2%	246.1 / 5.3	111	M301V (ATG→GTG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (36/53); new base G (16/3); total (53/58)
Fisher's exact test for biased strand distribution p-value = 7.05e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATG  >  NC_000913/353369‑353843

gcgcgGGTACCGGGGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAt                                                                                                                                                                                                                                                    <  2:688818/239‑1 (MQ=255)

    gggTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCg                                                                                                                                                                                                                                                >  1:141750/1‑239 (MQ=255)

      gTACCGGGGGCGTGGCTGGAATCCAACGAACCCTCCTGCATTCTCTACCCTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                                                                                                                                                                                                               <  2:1069642/238‑1 (MQ=255)

           ggTGGCGTGGCTTGAACCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACGCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTa                                                                                                                                                                                                                                            <  2:924741/236‑1 (MQ=255)

                          agccAACGAAACCTCCTGCATTCTCTACGCGTCCGGCACGACCGGCAAACCGAAAGGCGTGCATCGTGACGTCGGCGGATATGCGGTGGCGCGGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTg                                                                                                                                                                                                                          <  2:1184284/236‑1 (MQ=255)

                                      ctcctGCATTCTCTACCCTTCCGGCCCGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑gg                                                                                                                                                                                                           <  2:1148307/239‑1 (MQ=255)

                                       tcctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGa                                                                                                                                                                                                          <  2:489532/239‑1 (MQ=255)

                                        cctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑gg                                                                                                                                                                                                           >  1:1174181/1‑237 (MQ=255)

                                         ctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACt                                                                                                                                                                                                        <  2:549450/239‑1 (MQ=255)

                                         ctGCATTCTCTACACTGCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACt                                                                                                                                                                                                        <  1:1305995/239‑1 (MQ=255)

                                          tGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGGGGGGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGCCATTGGTTTATGAAggttgtctgccccggcgggccg                                                                                                                                                                                                          >  2:702949/1‑219 (MQ=255)

                                              ttCTCTACACCTCCGGCGCGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTgcggc                                                                                                                                                                                                   <  1:1256116/239‑1 (MQ=255)

                                              ttCACTACACTTCCGGCACGACTGGCAAACCTAAAGGCGTGCAGCGGGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTgcggc                                                                                                                                                                                                   <  1:487776/239‑1 (MQ=255)

                                                 tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCgtg                                                                                                                                                                                                <  1:216694/239‑1 (MQ=255)

                                                     cacTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTgtggt                                                                                                                                                                                            >  1:448966/1‑239 (MQ=255)

                                                          ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCCA‑TCGTTTACGAAGGATTGCCGACCCGGCCC‑GGAC‑gcggcggggtggggaca                                                                                                                                                                                       >  2:804600/1‑228 (MQ=255)

                                                          ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGGGgggtgggcc                                                                                                                                                                                       >  1:38526/1‑236 (MQ=255)

                                                           cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTA‑TCGGTTACGAAGGATTGCCGCACCGG‑CC‑GGACCGCGGGGTGTGGGGGaaaa                                                                                                                                                                                      >  1:1124115/1‑239 (MQ=255)

                                                               aCGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATAGGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAAtcgt                                                                                                                                                                                  <  2:950028/239‑1 (MQ=255)

                                                                 gACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTC‑TTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCga                                                                                                                                                                               <  1:900224/239‑1 (MQ=255)

                                                                   ccGGCAACCCTAAAGGCGTGCAGGGTGACGTCGGCGGATATGCGGTGGCGCTGGCGTCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCga                                                                                                                                                                               <  2:1341461/238‑1 (MQ=255)

                                                                   ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCgag                                                                                                                                                                              <  2:755558/239‑1 (MQ=255)

                                                                   ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCgag                                                                                                                                                                              <  2:141750/239‑1 (MQ=255)

                                                                           ccTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACg                                                                                                                                                                                                                                  <  1:182155/181‑1 (MQ=255)

                                                                            cTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCgtg                                                                                                                                                                                                <  1:376749/212‑1 (MQ=255)

                                                                             tAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGGCACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAgg                                                                                                                                                                   <  1:640773/239‑1 (MQ=255)

                                                                               aaGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGt                                                                                                                                                                  <  2:181601/238‑1 (MQ=255)

                                                                                aGGCGTGCAGCGGGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACGCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTa                                                                                                                                                                <  2:140929/239‑1 (MQ=255)

                                                                                   cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCCGCGGGTTCTTTTGCGCATCGGATATCGGGTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGc                                                                                                                                                              <  1:718800/238‑1 (MQ=255)

                                                                                   cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGc                                                                                                                                                              <  1:469786/238‑1 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGTGGGGGGCTA‑TCGTTTTCGCAAGATTGCCCGCCCTG‑CC‑GGGCTGGGGCGGGGGGGGGGCAAACGTCGGGAAAAA‑Tcgcggttggcgg                                                                                                                                                           >  2:753080/1‑228 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTA‑TCGGTTACGGAGGGTTGCCGGCCTGG‑GC‑GGGCTGCGGCGGGGGGGGGGCAATCGTCGGGAAATA‑TCAGGTTAGcccg                                                                                                                                                           >  1:1150902/1‑237 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCgg                                                                                                                                                           >  1:887086/1‑239 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGAACCCATTTTTGGCGGCAAAGCGGGCAGCTTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATAACGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGGCCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑GCAGGTTAGCCgg                                                                                                                                                           <  2:144197/239‑1 (MQ=255)

                                                                                      gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGGCCTGG‑CC‑GGGCTGCGGCGGGGGGGGGACAATCGTCGGGGAATA‑TTCGGTTAGCCggg                                                                                                                                                          >  1:1170317/1‑238 (MQ=255)

                                                                                      gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGGTTACGGAAGATTGGCGGCCTGG‑CC‑GGACTGCGGCGTGTGGTTGGCAATCGTCCGGGAATT‑TCAgggttgacggg                                                                                                                                                          >  1:955537/1‑230 (MQ=255)

                                                                                        aGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGAt                                                                                                                                                         <  2:851556/238‑1 (MQ=255)

                                                                                           gTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGtt                                                                                                                                                      <  1:1095284/238‑1 (MQ=255)

                                                                                            tGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTA‑TCCTTTACGAAGGGTTGCCGAACGGG‑CC‑GGGCTGCGGCGGGGGGGGGACACTCGGCGGGAAATA‑TTGGGTTGGCCGGGTGGtct                                                                                                                                                    >  2:241300/1‑239 (MQ=255)

                                                                                                    ggggATATGCGGGGGGGCTGGCGCCCTCGAGGGACACCATTTTTGGCGGCAAGGCGGGCGGCGTGTTCTTTGGCGCATCGGATATGGGCGGGGTGGTGGGGCATTCGTATATCGTGTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑cc                                                                                                                                            <  2:132768/236‑1 (MQ=255)

                                                                                                       gttaggCGGTGGCGCTGGCGCCTCCGATGGACACCTTTTTTGGCGGCAAAGCGGGAAGCGTGTTTTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑Ac                                                                                                                                        <  2:476594/233‑1 (MQ=255)

                                                                                                       gattggCGGTGGCGTGGGCGACCTCGATGGACACCATTGTGGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATAGCGGTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGGCCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑Ac                                                                                                                                        <  2:469581/233‑1 (MQ=255)

                                                                                                                 ggCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGGCTGCGGCGTGTGGTGGACAATCGGCGGGGAATA‑TCAGGTTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTcgcg                                                                                                                             >  1:1369334/1‑239 (MQ=255)

                                                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGGGAAAAA‑TCAGGGTAGCCGGGTGTTCTCAGCG‑CCG‑ACCCCCATTCGCGt                                                                                                                            >  1:1103793/1‑239 (MQ=255)

                                                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTCGCGt                                                                                                                            >  1:553286/1‑239 (MQ=255)

                                                                                                                     cTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGa                                                                                                                                                                                                                      <  2:274649/151‑1 (MQ=255)

                                                                                                                                  tGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTccc                                                                                                             <  2:887086/238‑1 (MQ=255)

                                                                                                                                          aTTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGGAAAAATTCCCTACCGCTGa                                                                                                    >  1:29868/1‑239 (MQ=255)

                                                                                                                                            tgttGGCGGAAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGTTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGaaa                                                                                                  <  2:1174181/237‑1 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTC‑TCGTTTTCGAAGGGTTGCCGACCCGG‑CC‑GGACTGCGGCGTGTGGTGGGCAAACGTCGAGAAATA‑TAAGGGTAGCCGGATGTTCTCCGCG‑CCC‑ACCCCCCTTTGCGGGCTGAAAAAAATCCCTACCGCTGCAATTCGCac                                                                                           >  2:701620/1‑238 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGaaa                                                                                                                     <  2:73102/213‑1 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGaaa                                                                                                                     >  1:73102/1‑213 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                            <  1:985771/238‑1 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGGGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATTGCGACTC‑TCGTTTACCCAGGAATGCCGACCCTG‑CC‑GGACTGCGGCGTGTGGGGGGCCATCCGCGAGAGATA‑TCCGGTTAGCCGGGTGTTTTCCGCG‑CCG‑GCCCCCCTTCCCCGGCCGGAAAAATTCCCTACCGCTGGAATTCTCaa                                                                                           >  2:1084678/1‑239 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCAGCGGATATCGGCTGGGTGGGGGGGCCTTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                            <  1:60135/238‑1 (MQ=255)

                                                                                                                                                               ggggttttCTTTTGCGCATCGGATACCGGCTGGGTGGGGGGGCATTCGTATTCCGTTTCCGGGCCGCTGCGGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGCCCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                               <  2:1249094/233‑1 (MQ=255)

                                                                                                                                                                  gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                               <  2:448966/236‑1 (MQ=255)

                                                                                                                                                                    gtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGc                                                                          <  1:1028603/239‑1 (MQ=255)

                                                                                                                                                                       cTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTgg                                                                       <  1:664675/239‑1 (MQ=255)

                                                                                                                                                                       cTTTTGCCCATCGGATTTCGGCTGGGGGGGGGGGCATTCGGATTGCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGGGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTgg                                                                       <  1:241300/239‑1 (MQ=255)

                                                                                                                                                                        ttttGCGCAGCGGATATCGGCGGGGTGGTGGGTCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGa                                                                      <  2:115180/239‑1 (MQ=255)

                                                                                                                                                                         tttGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGACTA‑TCGTTTACGAAGGATGGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGaa                                                                     <  1:804600/239‑1 (MQ=255)

                                                                                                                                                                             cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGAATGCGGCGTGTGGGGGACAATCGTCGGGAAATA‑TCAGGGTTGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTTCCTACCGCCGAAATTCTCACACACGAACCCTCGTCGCTGGAAGTg                                                                  >  2:348512/1‑238 (MQ=255)

                                                                                                                                                                             cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGGTTGCCGGCCTGG‑CC‑GGACTGCGGCGGGGGGTGGACAATCGTCGGGAAATA‑TTCGGGTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCCTTCGCCGGCTGAAAAAATTTCCTACCGCCTAAATTCGCCAACACGAtcctccgtccgtggaaagg                                                                  >  2:504674/1‑221 (MQ=255)

                                                                                                                                                                             cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGAATA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGt                                                                   >  1:115180/1‑237 (MQ=255)

                                                                                                                                                                                  cggaattCGGCTGGGTGGTGGGGCGCTCGATTATGGTTTACGCGCCGCTGCTGGGGGGGTTGGTGACTA‑TCGTTTACGAAGGCTTGCCGCCGTGG‑CC‑GGGTTGCGGCGTGTGGTGGACAATCGTCGAGAAAAA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTAt                                                            <  2:955537/233‑1 (MQ=255)

                                                                                                                                                                                              gggTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGGCAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCCAACACGATCTCTCGTCGCTGGGAGGGCTCTATCTGGCTTgaga                                                 >  2:96249/1‑238 (MQ=255)

                                                                                                                                                                                              gggAGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGAGTA‑TCGTTTAAGAAGGATTGCCGCACCGG‑CC‑GGGCTGCGGGGGGTGGGGGACAATCGGCGGGAAATA‑TCAGGGTTGCCGGCTGTTTTTCGGG‑GCG‑ACCGCCACTCCCGTGCTGACAAAATTCCCTACCCACGAAATTTGCAAACACGCACTCTCCTTGCTGGAAGTGCTTCATatggctgtcga                                                 >  2:63301/1‑234 (MQ=255)

                                                                                                                                                                                               ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTTGCGAATA‑TCGTTTACGGAGGATTTGCGACCTGG‑CC‑GGAGTGCGGAGGGTGGGGGAAAAACGTCGAGAAATA‑TCAGGGTCGCCGGATGTTTTCAACGCCCG‑ACC‑CCCTTAGCGGGGTCGAAAAATTTCCTTCCGCTGATAATTGCAAACATCATTTCTCGGCGCTGGactttcttctttctggatgggaa                                                >  2:1021081/1‑216 (MQ=255)

                                                                                                                                                                                               ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTA‑TCGTTTACGCAGGATTGCCGGCCTCG‑CC‑GGACCGCGGCGGGGGGGGGGCAAACCTCGGGGAATACTCGGGTT‑GCCGGATTGTTTCAGCG‑CCGAACCGCCTTCCGCG‑GCTTGAAAAATTCCCTACCCGCGAAATTGGCCAACCCGATCTCCGCTCGCGTGAAGGGCTCTCTtcggctggggca                                                >  2:122710/1‑234 (MQ=255)

                                                                                                                                                                                               ggtgggGGGGCAGTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATct                                                          <  1:545029/228‑1 (MQ=255)

                                                                                                                                                                                                   gtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCCAAACCGATCTCTCCTCGCTGGAAGTGCCCTATCTGGCTGGAGAAcc                                              >  1:358995/1‑236 (MQ=255)

                                                                                                                                                                                                    tggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTA‑TCGTTTACGAAGGGTTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGGGTAGCCGGGTGTTCTCAGCG‑CCG‑GCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGcgg                                          >  2:266781/1‑237 (MQ=255)

                                                                                                                                                                                                         cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGGCAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAAAACGATCTCTCGGCGCTGGCAGTGCTCTATCTGGCTGGGGAAACGCTGGaggg                                     >  2:1169728/1‑236 (MQ=255)

                                                                                                                                                                                                         cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCCGCG‑CCG‑ACCGCCCTTCGCGTGCTGGAAAAATTCCCTCCCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGCAGTGCTCTTTCTGGCTTGCGAAcctttctcttt                                     >  2:545029/1‑230 (MQ=255)

                                                                                                                                                                                                                atatCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                               <  1:137418/238‑1 (MQ=255)

                                                                                                                                                                                                                 tatCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAcc                              >  1:774453/1‑238 (MQ=255)

                                                                                                                                                                                                                  atCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTC‑TCGTTTTAGAAGGATTTCCGACCTGG‑CC‑GGACTGCGGCGTGGGGGGGACAATAGTCGAGAAATA‑TCAGGTTAGCCGGGTGGTCTCAGCG‑CCG‑GCCGCCATTCGCGGGCTGAAAAAATTCCCTACCCCTGGAATTCCCCAACACCATCTCTCGTCTGTGGAAGTGGCCCATCTGGGCGGTGAAACGCTGGGCGAGCCGcccc                             >  2:173509/1‑237 (MQ=255)

                                                                                                                                                                                                                   tCGTTTAGGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGCCCTGG‑CG‑GGACGGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGcc                           <  1:753080/239‑1 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGGGGGCGGCTA‑TCGTTTACGAAGGATTGCCGACCCGG‑CC‑GGACTGCGGCGTGGGGGGGAAAATCGTCGAGAAATA‑TCAGGGTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTCGCGGGCTGAAAAAATTCCCTACCGCTGAAATTCCCAAACAACATCTCTCGTCGCTGGAAGTGCTCTCTCTGGCTGGGGAACCGCTGGACGCGCCCACCCCCCGt                        >  2:1256575/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTTGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                        >  1:327712/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCCTTCCCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGGTCTATCTGGCTTGAGGAACGCTGGACGAGCCGACCGCCAGt                        >  2:1407156/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAAACGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑AACGCCATTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGGCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCCGGAGGAGCCGACCCCCCGt                        >  1:1085762/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGGTGTTCTCAGGG‑CCG‑ACCGCCATTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGGGAACCGCTGGAAGAGCCGACCGCCa                          >  2:1323198/1‑237 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGAATGCGGCGGGTGGTGGGCAATCGTCGAAAAAAA‑TCAAGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCCTTCGCGTGCTGAAAAAATTCCCTACAGGTGAAAATTGCCAAAACGATCTCCCGTCCCTGGAAGGGCTCTATCTGGCTGGGGGACCGCGGGACGAGCCGACCGCCacg                        >  2:1011485/1‑237 (MQ=255)

                                                                                                                                                                                                                      ttgccgcgCCGCTGCTGGGGGGGGTGGCGACTA‑TCGTTTACGAAGGATGGCCGCCCGGG‑CC‑GGACGGCGGCGGGGGGTGGACAACCGTCGAAAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                        <  2:1085762/235‑1 (MQ=255)

                                                                                                                                                                                                                        tACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGGAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGAGCTCTATCTGGCTGGAGGACCGCTGGACGAGCCGACCGCCAGTTg                      >  2:1272689/1‑239 (MQ=255)

                                                                                                                                                                                                                        tACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTAAGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑GCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGttt                      >  1:956813/1‑238 (MQ=255)

                                                                                                                                                                                                                         aCGCGCCGCTGGTGGGGGGGATGGCGACTA‑TCGTTTACGAGGGATTGCCGCCCTGG‑CC‑GGACTGGGGCGTGGGGGGGAAAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                     <  2:327712/239‑1 (MQ=255)

                                                                                                                                                                                                                         aCGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCCGCG‑CCG‑AACGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGGAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGCCCCCCAGTTgg                     >  1:492781/1‑239 (MQ=255)

                                                                                                                                                                                                                         aCGCGCCGCTGCGGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                     <  1:96249/239‑1 (MQ=255)

                                                                                                                                                                                                                         aCGCCCCGCTGGTGGTGGGGATGGCGACTA‑GCGTGACCGAGGGATTG‑CGTCCCGG‑CCTGGAGTGGGGCGGGGGGTGCACACTCGGGGAAAAATT‑TCGGGAAAGCATGCGGTCCGATGCC‑CCG‑ACCGCCATTGGCGGCCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCGCTCGTCGCTGGAAGTGCTCTATCGGGCTGGATATCCGCTGGACGAGCCGCCCGCCAGTTgg                     <  2:1097892/239‑1 (MQ=255)

                                                                                                                                                                                                                           gcgcCGCTGCTGGGGGGGATGGCGATTA‑TCGTTTAGGAAGGATTGCCGACCGGC‑CC‑GGACGGCGGCGTGTGGTGGACAATCGTCAAGAAATA‑TAAGGTTAGCCGGATGTCCTAAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  2:38526/239‑1 (MQ=255)

                                                                                                                                                                                                                           gcgcCGCCGCTGGCGGGTGGGGCGTCTA‑CCGTTTACGAGGGTGTGTCGACCTGG‑CG‑GGCCTGCGGGGTGTGGGGAACAATCTTCGAGAAATA‑CCAGGTTAGCGGGATGTCCCCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGAAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  2:135206/239‑1 (MQ=255)

                                                                                                                                                                                                                           gccccgcGGGTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGGGGTGGACAATCGTCGAGAAATA‑CCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  1:1084678/236‑1 (MQ=255)

                                                                                                                                                                                                                            cgcggtggcggggggggAGGGCAATTT‑TGGTTTAGGAGGGTTGGCCGCCCTGG‑CC‑GGACGGGGGGGTGTGGTGGACAATCGTCGAGAAATA‑TAAGGTTAGCCGGATGTCCTCGGCG‑CCG‑CCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  2:1257606/225‑1 (MQ=255)

                                                                                                                                                                                                                              ccgcTGCTGGCGGGGATGGCGACTA‑TCGTTTTCGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGGGTGTGGTGGGCAATCGGCGAGGAATA‑TCAGGGTAGCCGGATGTTCTCAGCG‑CCC‑GCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACCCGATCTCCCCGCCCTGGAAATGCCGTATCTGGCTGGGGCACCGCCGGACGAGCCGCACGCCCCTTGGGTggg                >  2:489961/1‑237 (MQ=255)

                                                                                                                                                                                                                               cgcTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGGGTGGTGGACAATCGTCGAGAAATA‑TCAGGGTAGCCGGGTGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCCATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGc               >  1:1257606/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gggggggTTGCCGACTG‑TCGTTAACGAAGGATGGCCGACCTGG‑CC‑GGGCTGGGGCGGGTGGTGGAGAATCGTGGAAAAAGT‑CCAGTTTAGCCGGATGTTCCCGGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg         <  2:898179/235‑1 (MQ=255)

                                                                                                                                                                                                                                            gATGGCGACTA‑TCGTTTTCGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGGGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCGGGAAAAGCTCTATCTGCCTGGGGAACCGCTGGACGAGCCCCCCCCCCAGTGGGTGCGCAATACGCTGGAtg  >  2:865327/1‑239 (MQ=255)

GCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTA‑TCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGGTTAGCCGGATGTTCTCAGCG‑CCG‑ACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATG  >  NC_000913/353369‑353843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`