Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 827,507 T→G 31.8% D492A (GAC→GCC)  ybhF ← putative ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913827,5070TG31.8% 178.7 / 16.2 98D492A (GAC→GCC) ybhFputative ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (9/53);  new base G (34/2);  total (43/55)
Fisher's exact test for biased strand distribution p-value = 1.05e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGTGGATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGA  >  NC_000913/827271‑827744
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
aGTCGTGGATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGTCa                                                                                                                                                                                                                                                     >  1:256452/1‑239 (MQ=255)
aGTCGTGGATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCa                                                                                                                                                                                                                                                     >  1:425103/1‑239 (MQ=255)
            aCTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCGCCGGAAgc                                                                                                                                                                                                                                         >  2:1159676/1‑238 (MQ=255)
            aCTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATAACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCAAGGCGGGCGGGGGGGGCAAcggcggtaacg                                                                                                                                                                                                                                        >  2:1140478/1‑234 (MQ=255)
                    aaGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtcgtc                                                                                                                                                                                                                                <  1:1323883/239‑1 (MQ=255)
                         ttGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGaa                                                                                                                                                                                                                           <  1:1256049/239‑1 (MQ=255)
                          tGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGCACCGCCGAAGTCGGGtcgggggggaa                                                                                                                                                                                                                          >  2:171138/1‑231 (MQ=255)
                          tGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCCAAGCCGCAGGCCGGGGCGTCGAGaaa                                                                                                                                                                                                                          >  2:523404/1‑239 (MQ=255)
                          tGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAACGCCGGGAGGAGGTtcgtctggaac                                                                                                                                                                                                                          >  1:150994/1‑233 (MQ=255)
                              ccATGGTGGGAGCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGa                                                                                                                                                                                                                      <  1:121370/239‑1 (MQ=255)
                                    tggGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTc                                                                                                                                                                                                                 <  2:425103/238‑1 (MQ=255)
                                    tggGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTc                                                                                                                                                                                                                 <  2:846497/238‑1 (MQ=255)
                                    tggGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTc                                                                                                                                                                                                                 <  2:297417/238‑1 (MQ=255)
                                      ggATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGGAACGGCGGCAGGCGGGTCGTCGAGAAAAAGAATGTACgg                                                                                                                                                                                                              >  2:1187695/1‑239 (MQ=255)
                                         tCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGTCAAAGCCGGAAGTCCGTTCGGCGGGAAACAGAATTGCCgggt                                                                                                                                                                                                            >  1:1126818/1‑236 (MQ=255)
                                           gggTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTCAAAGCCGTGAGTAGCTTCGTCGGGAAACAGAATGTCACGTTCaa                                                                                                                                                                                                         >  1:850270/1‑238 (MQ=255)
                                             gTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATg                                                                                                                                                                                                        <  2:1024164/238‑1 (MQ=255)
                                                 cTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCCACACCGGAAGTCGGGTCGTTGGGAAACAGAATGGCCGGGTCAttcctaa                                                                                                                                                                                                   >  1:227682/1‑233 (MQ=255)
                                                 cTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGACGGTTCGTCGAGAAACAAGATGGACGGTTCATGCATCa                                                                                                                                                                                                   >  1:1212831/1‑239 (MQ=255)
                                                  tCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCa                                                                                                                                                                                                   <  1:172421/238‑1 (MQ=255)
                                                  tCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCa                                                                                                                                                                                                   <  2:562774/238‑1 (MQ=255)
                                                      cGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGaa                                                                                                                                                                                             <  1:523404/239‑1 (MQ=255)
                                                       gTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCCGGAGGCGGGCTGTCGCGAAACACAAAGTCCGGGTCATTCATTAGCGCAc                                                                                                                                                                                            >  2:1100146/1‑239 (MQ=255)
                                                       gTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc                                                                                                                                                                                            >  1:1158570/1‑239 (MQ=255)
                                                           gCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGTCAACCCCCGGAGTCGGTTTGTCTCGAACCAACATCTCCGGTTCATTCCTCAGCGCACAgg                                                                                                                                                                                         >  1:1332062/1‑238 (MQ=255)
                                                           gCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGGACAgg                                                                                                                                                                                         >  1:1325426/1‑238 (MQ=255)
                                                           gCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCCGAAGTCGGTTCGTCGAGAACCAAAATGTCCGGGTCATGCATCAGCGACCAgg                                                                                                                                                                                         >  2:111381/1‑238 (MQ=255)
                                                                cTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGCGGGGGGCACCGCCGGACATCGGTTCGTCGTGAAACAGAAAGTCCGGGTCCTGCATCCTCGCACAGgccacc                                                                                                                                                                                    >  2:1178227/1‑236 (MQ=255)
                                                                cTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGGAGGCGGTTCGTCGGGAAAAAGAATGTCCGGGTCATGCCTCAGCGAACAGgcccac                                                                                                                                                                                    >  1:669398/1‑235 (MQ=255)
                                                                   tgCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCGGGAGTCGGTTCGTCGAGAAAAAGAATGTCCGGGTCCTTGATCAGCGAACAGgccagcgcc                                                                                                                                                                                 >  2:937437/1‑238 (MQ=255)
                                                                       ttCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCg                                                                                                                                                                            <  1:1159676/239‑1 (MQ=255)
                                                                       ttCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCg                                                                                                                                                                            <  2:717362/239‑1 (MQ=255)
                                                                       ttCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCg                                                                                                                                                                            <  1:1106710/239‑1 (MQ=255)
                                                                        tCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc                                                                                                                                                                           <  1:1178227/239‑1 (MQ=255)
                                                                           aaTCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGACGGGGGGGTGCCAGCCCGGAGTCAGTTCCCCGGGGGACAGAGTGGCCCGTGCCCGCACCAGCCAACAGgccggcgcccccccccct                                                                                                                                                                        >  2:382847/1‑230 (MQ=255)
                                                                               gTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaa                                                                                                                                                                    <  1:96381/239‑1 (MQ=255)
                                                                               gTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaa                                                                                                                                                                    <  1:182556/239‑1 (MQ=255)
                                                                               gTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaa                                                                                                                                                                    <  1:107925/239‑1 (MQ=255)
                                                                               gTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaa                                                                                                                                                                    <  1:687651/239‑1 (MQ=255)
                                                                               gTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAACGAATGTCCGGTTCATGCATCCGCCAACAGACCCGCGACAGCCGCAGTTTaa                                                                                                                                                                    >  2:638328/1‑239 (MQ=255)
                                                                                          cGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATgg                                                                                                                                                          <  2:600/238‑1 (MQ=255)
                                                                                          cGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCCGAAGTCGGTTCGTCCAGAAAAAGAAAGTACGGGTCCTGCATCAGCGAGAAGGCCAGCGCCCGCCGCTGGTTAAAACCTCAttga                                                                                                                                                         >  2:999096/1‑236 (MQ=255)
                                                                                             tGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAg                                                                                                                                                       <  1:685094/238‑1 (MQ=255)
                                                                                                           ccGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTg                                                                                                                                       <  1:1100146/239‑1 (MQ=255)
                                                                                                                gTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGGACAAGCCAGCGCCCGCCGCTGTTTTAAAACTAATTGCCGTTCATCGGTGGCGGGGGAgg                                                                                                                                  >  1:1008200/1‑239 (MQ=255)
                                                                                                                 tACACCAGGCCGATGCGGTCGCCATATTCCGCTTCATCCATAAAGTGGGTCGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCGAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAgg                                                                                                                                  <  2:922179/238‑1 (MQ=255)
                                                                                                                 tACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAgg                                                                                                                                  <  1:1187695/238‑1 (MQ=255)
                                                                                                                         gCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAAAGGCAGTTCATCGGTGGCGTGGGAGGCGATACttt                                                                                                                        >  1:802350/1‑239 (MQ=255)
                                                                                                                         gCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGGGAAACAGAAATGCCGGTTCATGCATCCACGGAAATGCCAGCGCCAGCCGCTGTTTTAAAACTAATGGACGGTCATCGGGGGGGTGGGGGGGGGAACttt                                                                                                                        >  2:888965/1‑239 (MQ=255)
                                                                                                                           cGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGAAGGCGGGTCGTCGAGGAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCCGCGCCCGCCCCTGTTTATAACCCAATTTGAGATCATCGGTGGGGGGGGGGGCGGTACttttt                                                                                                                      >  2:402076/1‑238 (MQ=255)
                                                                                                                             aTGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCCGCGCCAGCCGCTGTTTAAAACCTAATTGCAGGTCATCGGTGGCGGGGGGGGCGGTACtttttcg                                                                                                                    >  1:1125950/1‑236 (MQ=255)
                                                                                                                             aTGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGTGCACCCCGGGAGTCGGTTCGTCGAGAAACAGAAAGTGCGGTTCATACCACACCGAAAAGGCCCGCGCCCGCCGCCGTTTTATACACAATCGCACGTCATCCGGGGCgggtggggaggtagtgttctc                                                                                                                     >  2:1033893/1‑219 (MQ=255)
                                                                                                                             aTGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTTAAAACTAAAGGCAGTTTATCGGTGGCGGGGGAGGCGATACtttttcg                                                                                                                    >  1:1012948/1‑236 (MQ=255)
                                                                                                                               gCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAAAGCCCGAAGTCGGGTTGTCGAGAAAAAGAATGTCCCGGTCATGGATCAGCGAACAGGCGCGCGCCAGCCGCCGTTTAAAACATAATAGCAGGTCATCGGGCGCGCGGGGGGGGGGACATTTCAgg                                                                                                                   >  2:373633/1‑238 (MQ=255)
                                                                                                                                  gTCGCAATATTCCGCTTCACCCATAAAGTGGGTGGGGACCATCACCGTGACGCCTTTCGCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCg                                                                                                                <  2:992822/238‑1 (MQ=255)
                                                                                                                                    cGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGaa                                                                                                              <  1:236400/238‑1 (MQ=255)
                                                                                                                                       aaTATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAg                                                                                                                                                       <  1:507803/196‑1 (MQ=255)
                                                                                                                                        atatTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCt                                                                                                         <  1:1033893/239‑1 (MQ=255)
                                                                                                                                                   cATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGTGGGGGGCCACGACGGAAGGAGGGTCGTCGAGGAACAGAATGGTCGGGTCATGCCGCAGCGAACAGGCCAGCCGCCGCCGCTGCTTAAAACCGCATTGCACTTTCTGGGGGGCGGGGGGGGGCGGTCTTTTCAAGGCGCACGCGTCGCGCGTGCgg                                                                                             >  2:762919/1‑239 (MQ=255)
                                                                                                                                                   cATCCAGAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCgg                                                                                             <  1:946012/239‑1 (MQ=255)
                                                                                                                                                     tCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCCACGCCGGAAGTCGGTTTGTCCAGCAACAGGAAGTCGCGCTCATGCCCCAGCGAACAGGGCAACGCCAGCCACCCTTTTACACCCAATAGCTGTGCCACGGTGGCGGGGGAGGCGATAACTTTTCGGCCGAAAGCCTCGCCCCTTCgggg                                                                                           >  2:1076434/1‑238 (MQ=255)
                                                                                                                                                                ggtggtGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCCACACCCGGAGATGGTTCGGCGTGAAAAAGAAAGTTCGGTTTATTGATCAGCGAACAAGCCCCGGCCAGACGCCGTTTATAACCTAATGGCAGTTCACCGGTGGGAGGGGCGGGGCGAGTTTTTGAGGCGCACGCCGCGCTCGTGCGGGGGAGATTTTTGt                                                                               >  2:191628/1‑239 (MQ=255)
                                                                                                                                                                ggtggtGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGTCAAAGCCGGAAGTCGGGTCGTCGGGAAACAGAATGGTCGGGTCATGCATCAGCGAAAAGGCCGCCGCCCCCCGCTGGTTTAAACCTAATGTCAGTTTTTCGGGGGGGGGGGGGGCGGGATTTTTTAGGCCCAAAGCCCCGCTCaccggggggggttttttgt                                                                                 >  2:745613/1‑221 (MQ=255)
                                                                                                                                                                       aCCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGGTTATCGGTGGCGTGGGGGGCGATACTTTTTAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTgcg                                                                       >  1:1113921/1‑238 (MQ=255)
                                                                                                                                                                          aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCg                                                                   <  1:638328/239‑1 (MQ=255)
                                                                                                                                                                           tCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGa                                                                  <  1:385330/239‑1 (MQ=255)
                                                                                                                                                                                 ggACGCCTTCCTCTACCGTGTTGTTGATGTGCAGCAAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGt                                                            <  1:1254630/238‑1 (MQ=255)
                                                                                                                                                                                  gtcGCCTTTCGCTCCCAGGCTGTAGATGGGGAGCCAAAAGTCACGGCGGGTGAGGGGGTCGACGCCGGAAGTCGGGTCGTCGAGAAACAGAAAGGCCGCTTCAGGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTGTAAAACCTAAGGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCTGGAGAGTTTTTCGTTCTGCGCCCGACCTCGt                                                            <  2:328404/236‑1 (MQ=255)
                                                                                                                                                                                  gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGt                                                            <  1:111381/238‑1 (MQ=255)
                                                                                                                                                                                  gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGt                                                            <  2:256452/238‑1 (MQ=255)
                                                                                                                                                                                  gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGt                                                            <  1:1140890/238‑1 (MQ=255)
                                                                                                                                                                                  gACGCCTTTCGCTTCCATGCCGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAACGTCCGGTTCATGCAGCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGATGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCTACCGCGt                                                            <  2:363223/238‑1 (MQ=255)
                                                                                                                                                                                    cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAg                                                         <  1:999096/239‑1 (MQ=255)
                                                                                                                                                                                    cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAg                                                         <  1:1140478/239‑1 (MQ=255)
                                                                                                                                                                                     gCCTTTCTCTACCATGCTGGTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAg                                                         <  2:58620/238‑1 (MQ=255)
                                                                                                                                                                                       cTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGcc                                                       <  1:171138/238‑1 (MQ=255)
                                                                                                                                                                                         ttCTCTACCATGCTGTTGATGTGCAGCCAAAATGCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATa                                                    <  2:53665/239‑1 (MQ=255)
                                                                                                                                                                                         ttCACTACCATGCTGTTGACGTGCATCCAAAATTCGCGGCGGGTGAGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAACCCGAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCCCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATa                                                    <  2:272700/239‑1 (MQ=255)
                                                                                                                                                                                           ctctACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATaca                                                  <  1:882090/239‑1 (MQ=255)
                                                                                                                                                                                                 cATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTTAAAACTAATGGCAGTTTATCGGTGGGGGGGGGGGGGAGACTTTTTAAGCCGCAAGCCCCGCTCATGCGGGGGATTTTTTCGTTTTGCGCCCGGCCGCGTGAGCCCTACACACCaa                                            >  2:170642/1‑238 (MQ=255)
                                                                                                                                                                                                   tGCTGTTGATGTGCAGCCAAAATTCACGGCGGATGTGGGGGGCAAAGTCGGGAGGCGGGTCGTAGAGAAACAGAATGTGCGGTTCATGAATTATCGAAACAGGCAGGGGCCCGCGCACTTTAACACCCAAATGCAGGTTAACGGGTGCGGGGGGGACGGTCCTTTTTCAGCCGACCGCCCCGCCCCTGCGGGGGGGTTTTTTGTTTCTCCCCCCGCCGCGCTAGCGATacacacacgac                                          >  2:199390/1‑234 (MQ=255)
                                                                                                                                                                                                    gCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCAt                                                                                                                                                                                                     <  1:330546/90‑1 (MQ=255)
                                                                                                                                                                                                     cTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCCACGCCGGAAGTCGGTTCGTCGAAAAACAAAAAGTCCGGTTCATGCATCAGCGAACAGGGCCCCGCCAGCCCCCGTTTTAAACCTAATGGCCGTTCATCGGCGGGGTGGGGGGCGGTACTTTTCATGCCGAAAGCCACGCTCCTGTGGGGGATTTTTTATGTTTCCGCCCGACCGCGTAAGCCCTACAACCCCGAGaa                                        >  2:810424/1‑239 (MQ=255)
                                                                                                                                                                                                     cTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGGAGTCGGGTCCTCGAGAAAAAGAATGTCCGGGTCATGCATCAGCGAACAGGCCACCGCCCGACGCCGTTTAAAACCTAATGGCAGTTCATCCGGGGCGGGGGAGGGGGTAATTTTCTGGGCCGACGCCCCCCTCCTGCGGGGGAATTTTTCGTTTTGTCTCCGGACCCGCTCGACATACACACCagagga                                        >  2:1148645/1‑237 (MQ=255)
                                                                                                                                                                                                      tGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAg                                                         <  1:27191/221‑1 (MQ=255)
                                                                                                                                                                                                       gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCAAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGaaaa                                      <  1:593272/239‑1 (MQ=255)
                                                                                                                                                                                                          gATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGGGAACCCCGGAAGTCGGGTCCGCGAGAAACAGAAAGTCCGGGTCACGCATCAACGGACAAGCCCGCGGCCGACGCTGTTTTAAAACCAAAGGGAGTTCATCGGGGGGGGGGGAGGGGAGACTTTTTCGGCCGCAAGCCCCCCTCCTGCGGGGGATTCTTTCGTTCTCGCGCCCCGCGCGTCAGCCACTCACACCAGTGaaaaaa                                    >  2:1287407/1‑237 (MQ=255)
                                                                                                                                                                                                             gtgCAGCCAAAATTCACGGCGGGTGAGGGGGGCAACGCCGGAAGGCGGGTCGTCGAGAAAAAGAATGTCCGGTTCATGCCTCAGCGAACAAGCCCGCGCCCGCCGCTGTTTAAAACCAAATGGGCGGTCCTCGGGGGCGCGGGGGGGGAGTCTTTTCAGGCCGAACGCCCCGCCCATGCGGGGGATTTTTTTTGTTTTGCCCCCACCCCCGAAGCCATACACACCACGGaaaaacagga                                >  2:1229073/1‑234 (MQ=255)
                                                                                                                                                                                                                aaggacaaaTTCACGGCGGGTGAGGGGGTAAACGCCGGAAGTCGGAGCGACGAGAAACAGAATGTCCGGTTCAGGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTACTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTaaa                              <  2:574449/232‑1 (MQ=255)
                                                                                                                                                                                                                  gCCAAAATTCACGGCGGGTGAGGGGGGCCACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGTCCGGGTCATGCATCAGCGGACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGGTCATCGGGGGCGTGGGGGGGGCTACTTTTCAGGCCGAACCCCTCCCTCCTGCGGGGGGTTTTTTTTTTCTGCGCCCGCCCCCGTAAACCCACCACACCCGAGAAAAAAAGGAAAttt                           >  2:1202289/1‑238 (MQ=255)
                                                                                                                                                                                                                      aaaTTCACGGCGGGTGAGGGTCTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGtt                       <  1:463273/239‑1 (MQ=255)
                                                                                                                                                                                                                      aaaTTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGtt                       <  1:888965/239‑1 (MQ=255)
                                                                                                                                                                                                                      aaaTTCACGGCGGGTGAGGGGGTCAACGACGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGtt                       <  1:946121/239‑1 (MQ=255)
                                                                                                                                                                                                                        aTTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCg                     <  1:417437/239‑1 (MQ=255)
                                                                                                                                                                                                                             cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGt                <  2:1325426/239‑1 (MQ=255)
                                                                                                                                                                                                                             cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGGGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGt                <  2:227682/239‑1 (MQ=255)
                                                                                                                                                                                                                                cggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCa              <  1:937437/238‑1 (MQ=255)
                                                                                                                                                                                                                                   gggtggggggTCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAATGGCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAgg            <  2:1308590/233‑1 (MQ=255)
                                                                                                                                                                                                                                             tCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTaga  >  1:1189384/1‑238 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                     
AGTCGTGGATCAACTGAATAAAGGCTTGCTCCATGGTGGGATCGGGTTGCTCATCGTTAGCCGACTGTGCTTTCAAATCGTCCGGCGTGCCGCTGGCGATTAATTTCCCGCGGTACACCAGGCCGATGCGGTCGCAATATTCCGCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAAACCTAATGGCAGTTCATCGGTGGCGTGGGAGGCGATACTTTTCAGGCCGAACGCCTCGCTCATGCGGGAGATTTTTTCGTTCTGCGCCCGACCGCGTAAGCCATACACACCAGAGAAAAAGCGTAAATTCTGTTCGACCGTCAGGTTACCGTAGA  >  NC_000913/827271‑827744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: