Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 989,945 A→G 15.5% S138P (TCA→CCA)  pncB ← nicotinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913989,9450AG15.5% 140.3 / 2.5 80S138P (TCA→CCA) pncBnicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (28/37);  new base G (5/9);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGA  >  NC_000913/989711‑990183
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
cctccGGCGTGAGGGAAAGCCGCCGCGCCAGATCGTAGTTTCTGGTTCCCACAAACCAGGATTCCTGTAGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcga                                                                                                                                                                                                                                                <  2:392466/235‑1 (MQ=255)
ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcga                                                                                                                                                                                                                                                <  1:106280/239‑1 (MQ=255)
ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcga                                                                                                                                                                                                                                                <  2:848897/239‑1 (MQ=255)
ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcga                                                                                                                                                                                                                                                <  2:1349062/239‑1 (MQ=255)
  aTCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTTAAGCCGAGAAGTCGGCTAATTTGCTTTTCCGCGTGTCGGGGGGTTTGGCCACGTCGGCCCGCGGGGaggggta                                                                                                                                                                                                                                              >  1:386035/1‑234 (MQ=255)
  aTCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGGGAAGACGACCAATTTGCTTTTCAGCGCGTCGAGGGGTTTCGCAACGTCGGCCGCCGGTGggcgata                                                                                                                                                                                                                                             >  1:884048/1‑239 (MQ=255)
       cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGCTGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGCGAAGTCGGACAATTTTTTTTTCAGCGTGTGCGGGGCTTGCGCAACATCCGCCTGCGGTGggcggtatcgat                                                                                                                                                                                                                                       >  1:531185/1‑239 (MQ=255)
       cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGagcgatagcga                                                                                                                                                                                                                                        <  1:1268279/238‑1 (MQ=255)
        gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                      <  2:1184633/239‑1 (MQ=255)
        gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATg                                                                                                                                                                                                                                      <  1:660447/239‑1 (MQ=255)
         tGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCCGAAGTTGACCAATTTTCTTTTCAGCGTGTCGCGGGCTTGCGCAACCCCGGCGCGCGGTGggggatagcgtagt                                                                                                                                                                                                                                     >  1:261511/1‑235 (MQ=255)
              gAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCa                                                                                                                                                                                                                                 <  1:988885/238‑1 (MQ=255)
                                    aGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGCGAAGTCGACTAATTTGCTTTTCAGCGGGTCGAGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTCTCTGCTAAACGCCCGCaaa                                                                                                                                                                                                          >  2:1103907/1‑239 (MQ=255)
                                           ggTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTTACCACGAGAAGGACAATAATTTGCTTTCCAGCGTGCCGCAGGCTTGCGCCACGCCGCCCCGCGGTGTGCGGTAGCGGTGTCACATTTTACTGATAACCGCCAGCaacgacact                                                                                                                                                                                                    >  2:7315/1‑231 (MQ=255)
                                              gCCCACAACCCAGGATTCCTGTGGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAAAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTcc                                                                                                                                                                                                 <  1:499147/238‑1 (MQ=255)
                                              gCCAACAAACCAGGATCCCGGTGGAAGCCGCTTAACGATGTTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGAAATATCAAGCCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTcc                                                                                                                                                                                                 <  2:587505/238‑1 (MQ=255)
                                               cccACAAACCAGGTTTCCTGTTGAGGACGCTTAAGGATGTTTTCTTGTACTTCGCGAGAAAAACGGCGAGGGGGGCAAAAATCCATCAGAGGGAAGCGCGACATATCAAGACCGGCGGTTAACGCGGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGAAACGTCGGCCGGGGGTGAGCGATAGCGATGTACCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCa                                                                                                                                                                                               <  2:238203/239‑1 (MQ=255)
                                                              tCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGTCGCGGGCTTGCGCAACGTCGGCCCGCGGGGAGCGATAGCGATGTACCCTTTTCCTGTCAACCGCCAGGAAAGGAAATTCCCCGAGGAGGACTTCAc                                                                                                                                                                                 >  2:908378/1‑238 (MQ=255)
                                                              tCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGCGAAGGCGAACAATTTTCTTTTCAGGGTGTCGAGGGCTTTCGCCACGTCGGGCTGCGGGGGGCGGTAGCGATGTACCCTTTCACTGATAAACGCCAGCAAAGGAAATTCCCCGAAGAAGACtttcc                                                                                                                                                                                 >  2:1191028/1‑235 (MQ=255)
                                                                    tGCAGCCGCTTAACGATGGTTTCTTGTCCTTCGCGAAAAAAACGGCGAGGGGTGCCAAAATCCATAAGAGGGAGGCGCACCATAACAAGCCGGGCGGTTACCGCGGAGAAGTCGATTAATTTGCTTCCCGGCGTGTCGAGGGTTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACg                                                                                                                                                                           <  2:26679/238‑1 (MQ=255)
                                                                    tGCAGACGCTTAACGATGTTTTCTTGTACTGCGAAAAAAAAACGGCGCCGGGTGCCAAAACCGATCAGATGGAAGCGGGACATATCAAGCCCGGCGGTTAACGCCGAGACGTCGACTATTTTGCTTCCGAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGGGCGATAGCGATGTCCCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACgg                                                                                                                                                                          <  2:82009/239‑1 (MQ=255)
                                                                     gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTTACGCCGAGCAAGCGACTAATTTGCTTTCCAGCGGGGCGGGGGGCTGCGGAACGGCGGCCTGCGGTGAGCGATAAGCAGTGACCCTTTTACTGATAACCGCCAGCAAAGGGACTTCCCCGCGGATggatttcccccaccg                                                                                                                                                                          >  2:195995/1‑224 (MQ=255)
                                                                     gCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATACCAAGACCGGCGGTTAAAGCCCAGAAGTCGACCAATTTGTTTTCCAGCGGGTTGGGGGGTTGCGACAACTCGGCCTCCGGGGGGCGATAGCAGTGTGCCATTTTCCTGGTAAACGCCAGCAAAGGAACCTCCCAGAGGACGGCTTCCcaccactg                                                                                                                                                                          >  2:363273/1‑236 (MQ=255)
                                                                           gataacggttGGTTTTTTGATCTTCGGAAAAAAACCGTGCACGGGTGCAAAAATCCATAAGATGAAGTCGCAAAATAGCAAGACGGGCGTTAACGGCCGAGAGGCCGACTATTTGGCTTTCCAGCGTGTCAGGGGCTTGCGAATCGTCGGCCTGCGGTGGGCATTGGCAATGTCCCTTTCCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCtt                                                                                                                                                                  <  2:1125025/230‑1 (MQ=255)
                                                                               aaCGATGGTTTTTTGTACTTGGAGAAAAAAACGGCGAGGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGCCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTGGAGGGCTTGCGAAACGTCGGCCTGGGGTGAGCGATAGCGATGTCCCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAAc                                                                                                                                                              <  1:557080/239‑1 (MQ=255)
                                                                                     ggTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGGGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAAAGGCGGCCTGCGGTGAGCGGTAGCGATGTACCATTTTACTGATAACCGCCAGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAata                                                                                                                                                         >  1:641121/1‑238 (MQ=255)
                                                                                     ggTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAAACGACTAAATTGCCTTTCAACCGGTCGAGGGGTTGCGCACCGTCGGCCCGCGGGGAGGGCTAGAGGTGGACCCTTTTTCTGATAACCGGCCGAAAAGGGACCTCCCAGCGGGAGAATTTACGCCACGGGGCGCTTAAAAGAata                                                                                                                                                         >  2:510409/1‑238 (MQ=255)
                                                                                         tCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTTCTTTTCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGGTGTACCATTTCACCTATAACCCCCAGCAAAGGAACTTTCCCGAGGGTGACCTCACGCCACGGGCCGCTTTAACCAAAATccc                                                                                                                                                     >  2:455202/1‑237 (MQ=255)
                                                                                         tCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGACTAATTTGCTTTCCAGCCGGTCGAGGGCTTGCGCAACCTCGGCCCGCGGTGGGCGCAAACGATGTACCCTTTTCATGTTAACCCCCAGCCAAAGAACTTCCCCCAGGATTACTTCCCCCCACGGGCCCCTTAAACgccaacccc                                                                                                                                                     >  2:625725/1‑230 (MQ=255)
                                                                                          cTTGTACTGCGCGAAAAAAACGGCGAGGGGTGCAAAAATCCATCAGAGGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTCGAGGGCTTGCGCAACGTGGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGc                                                                                                                                                   <  1:1103907/239‑1 (MQ=255)
                                                                                                 tgcgagagaAAAACGGCGAGGGGGCACAAACCCAGACAGGTGAACGCGGGAAATATCAAGCCGGGGGGTAACCGCCGAGAGGCCGTCTAATTTTCTTTCCAGGGTGTCGAGGGTTTGCGAACCGTCGGCCGGGGGTGGGCGATGGCGATGTACCTTTTCCCTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                            <  2:733590/234‑1 (MQ=255)
                                                                                                 tccgggaaaaaaaCGGCGCCGGGTGCCAAAATCTATCAGATGGAAGCGCGACATATCAAGACGGGCGGTAAGCGCCGAAATGTCGACAAATTCTCTTGCCAGCGTGTCGAGGGCTTCCGCAACGGCGGCCGGCGGTGAGCGATAGCGATGTCCCATTCCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                            <  2:47946/231‑1 (MQ=255)
                                                                                                 tccgcgAAAAAACCGGCGCGGGGGGCCAAAGCCCTACCGAGGGAACCGCGAAATAACAAGCCCGGCGGGTACCCCCTAGATTCCGACAAATTTCTTTTCCAGCGTGGCGAGGGTTGGCCCACCGTGCGCCTGCGGTGGGTGATAGCGATGTCCTTTTCCACGGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCAt                                                                                                                                            <  2:252479/237‑1 (MQ=255)
                                                                                                   cgcgGGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGGTGGCTAATTTGCTTTCCAGCGGGTGGAGGGCTTGCGCCACGGCGGCCCTCGGGGGGCGGTATGGCTTGGACCTTTTACTGATAAACGCCCGCCAAAGAACGTCACCAAGGGTGGCATTCCGCCACCGGCCGCCTTaaaaaaaaaacacgcttcccata                                                                                                                                           >  2:754975/1‑219 (MQ=255)
                                                                                                   cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGGGGGGTTGCGCAACGTCGGCCCGCGGGGAGCGCGAGCGATGGACCATTTTCCTGATAACCCCCCGCAAAGGGACTTTCCCGAGGGTGACTTCACGCCCCCGGCCGCTTAAAACAATAACCacccttccctct                                                                                                                                          >  2:1294113/1‑228 (MQ=255)
                                                                                                   cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGACTAATTTGCTTTCCCGCGTGTCGGGGGCTTGCGCAACGTCGGCCTGCGGGGAGCGATAGCGATGTACCATTTTACTGATAAACGCCAGCAAAGGAACTTCCCCGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAATATCCAACTTGCCCTTa                                                                                                                                          >  1:1160199/1‑239 (MQ=255)
                                                                                                   cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGGTTTTCAGCGTGTCCGGGGCGTGCGCCACGTCGGGCTGCGGTTAGCGAGAGCGATGTACCATTTTAATGATAACACCCAGCCAAGGAACTTCCCAGAGGATGGCGTTACGCCACGGGCCGCTTAAACGAAAAACCAACCTGCCagtt                                                                                                                                          >  2:291933/1‑236 (MQ=255)
                                                                                                   cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGCTAACGCCGCGAAATCGGCTAAATTTCTTTCCCACGTGTCGAGGGTTTGCGCAACGCCCGCCTTCGGTGAGCGACAGCGCTGGACCATTTGCCTGATAACCGCCAGCATCAGAACCTCCCAGAGGGTGACTTCCTCCCCCAGGCCGCCTTCACGAATACTGACCCTGCCATTa                                                                                                                                          >  2:1232069/1‑239 (MQ=255)
                                                                                                        aaaaacCGGCGACGGGTGCCAAAATCCATAAGATGGAGGCGCGAATTACAAAGGCGGGCGGTTAACGCCGAAAAGTCGACTAATTTGTTTTCCAGCGTGCGGAGGGCGTGCGAAACGCCGGTGGGCGGTGAGCAATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGt                                                                                                                                      <  2:641121/237‑1 (MQ=255)
                                                                                                         aaaacCGGCGGCGGGGGCAAAAACCCAGCAAGTGGAGGCGCGAAAAATCAAGCCGGGCGTTTACCGCCGAAAGGTCGACTAATTTGCTTTCCAGGGTGTGGAGGCTTTGCGAAACGTCGGCCGGGGGGGAGCGATGGCGTGGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTg                                                                                                                                    <  2:71015/239‑1 (MQ=255)
                                                                                                          aaaaCGGCGACGGGTGCCAAAACCCATCAGAGGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTgg                                                                                                                                   <  1:455202/239‑1 (MQ=255)
                                                                                                               ggcggcGGGTGCCAAAATCCATCAGAAGGAAGCGCGACATAACAAGACCGGGGGTTAACGCGGAGAAGCCGACTAATTTGCTTCCCAGCGGGGCGAGGGCTGGCGCACCGTCGGCCGGCGGTGAGCGATAGCGATGTACCATTCCACGAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACg                                                                                                                              <  2:1190240/239‑1 (MQ=255)
                                                                                                                gcggcgGTTGCAAAAACCACTAAGAGGGAGCCGCGACATAACAAGCCGGGCGGTTAACGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGCCGAGGTTTTGCGCACCGTGGGCCTGCGGTGAGCGTGAGCGATGTCCTATTTCATTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATAGCCAGCTTGCCATTATCGTTGGACACg                                                                                                                              <  2:1315994/238‑1 (MQ=255)
                                                                                                                   gagggggGCAAAAACCCATCAGAGGGAAGGGAAACATACCAGGCCCGGGTGTTACCGCCGAAAAGCCGATTAATTTGCTTTCCGGGGTGTCGAGGGTTTGCGAACGGTGGGCCTGCGGTGGGAGTTAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGt                                                                                                                            <  2:731928/235‑1 (MQ=255)
                                                                                                                           ccAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGCGTTGAGGGGTTGCGCCACGTCGGCCCGCGGTGAGCGATAACGATGTACCATTTTACTGATAACAGCCAGCAAAGGCACTTCCCACAGGATGACTTCACCCCCCGGGCCGCTTAAACCAATATCCAGCCTGCCCTTTTCGTTGGACAACGGGACTtttt                                                                                                                   >  2:767963/1‑238 (MQ=255)
                                                                                                                            aaaaaCCCATGAGATGGAAGCGGGACATATCAAGACCGGCGTTTACCGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTCGAGGGCTTGCGAAACGTCGGCCTGGGGTGAGCGATAGCGATGTACCTTTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTc                                                                                                                  <  2:386035/237‑1 (MQ=255)
                                                                                                                                 tCCATCAGATGGAAGCGCGACAAAGCACGCCCGGTGTTTACCGCCGAGAAGTCGACTATTTTGCTTTCCGGCGTGCCGAGGGCTTGCGCACCGTCGGCCTGCGGTGAGCTATAGCGATGTCCCATTCCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGtt                                                                                                            <  2:150269/239‑1 (MQ=255)
                                                                                                                                  ccATCAGATGGAAGCGCAACTTATCAGGCCCGGCGGTTACCGCCGAGAAGTCGCTTATTTTGCTTCCCGGGGTGTGGAGGGCTGGCGCAACGCCGGCCGGCGGGGAGCGATGGCGAGGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTa                                                                                                           <  2:1160199/239‑1 (MQ=255)
                                                                                                                                   cATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCCAGAAGTCGAATAATTTGCTTTTCAGCGTGGCGAGGGGTTGCGCCACGTCGGCCCGCGGGGAGGGGTAGCGATGTACCATTTCCATGATAACCCCCCGCACAGGGAATTTCCCGAGGATGGCTTTACGCCACGGGCCCCCTAAACGAAAATCCAGCTTGCCCTTCTCGTTGGACACGGCGCCTTTTTCCGGGGTac                                                                                                          >  2:1287093/1‑238 (MQ=255)
                                                                                                                                            ggAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGGTTGCGCAACGGCGGCCTGCGGTGAGCGGTAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCCTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAACGGa                                                                                                    >  1:1022130/1‑237 (MQ=255)
                                                                                                                                             aacgcggcaCATAGCAAGCCCGGCGTTAACCGCCGAGAAGGCGACTAATTTGCTTCCCGGGGGGTCGGGGGCTTGCGCAACGTCGGCCGGGGGTGAGCGATAGCGATGTACCATTCCCCTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGaa                                                                                                   <  2:884048/229‑1 (MQ=255)
                                                                                                                                              aaGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAATCGACTAATTTGCTTTTCCGCGGGTCGAGGGCCTGCGCAACGGCGGCCTGCGGGGGGCGGGAACGATGTACCCTTTTACCGATAACCGCCCGCCAAGGAAATTCCCCCAGGATGACTTCACGCCACGGGCCGCGTAAACGAAAATCCCCATTGCCCTTATCTGTGGACACAGGGGCATTGTCCGGGGTTAAGCGGGAcac                                                                                                >  2:289260/1‑237 (MQ=255)
                                                                                                                                                    gcacgatcagggcgggtggTTAACGGCGCGAACGCGACTAATCTTCTTTCCAGCGTGGCGCGGGCTTCCGAAACGCCGGGCTGCGGTGAGCGAGAGCTATGTACCTTCTCTCTGATACCCGCCGGCAAAGGAACTTCCCAGAGGATGGCTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:306707/222‑1 (MQ=255)
                                                                                                                                                    gaaagctcacggcgggcggTTAGCGCGGAGAAGTCGCCTAATTTGCTTTCCGGCGTGTCGAGGGCTGGCGAAACGCCGTCCTGCGTGGAGGAATGGCGAAGAACCAGTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:531185/225‑1 (MQ=255)
                                                                                                                                                    gACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCGGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGTTGAGCGATAGCGATGTACCATTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  1:1175243/239‑1 (MQ=255)
                                                                                                                                                     caatatCAGGCCCGGGGGTTAACGCCGAGAAGTCGACAAATTTGTTTCCCAGCGTGTGGGGGGTTGGCGCAACGTCGCCCGGGGGTGAGCGAGAGCGATGTACCATTTCACTGAAACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:139925/236‑1 (MQ=255)
                                                                                                                                                     acaaaacAAGACCGGGGGTTAACGCCGAGAAGTAGCTTAATTTGTTTCCCAGCGTGGCGAGGGCTTGCGAAACGCGGGCTGGGGGTGGGCGATAGCGATGTCCCATTCCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:314892/232‑1 (MQ=255)
                                                                                                                                                      agaatagcgccggggggTTAACGCGGAGATGTAAACGATTTCGCTTTCCGGCGTGGGGGGTGCTTGCGCAGCGTCGGCCGGCGGTGGCGGATAGTGATGTACAATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa                                                                                          <  2:897168/222‑1 (MQ=255)
                                                                                                                                                         atCAAGACCAGCGCTTAAGCCCAAAAACTCGTGCAATTTGTTCTCCTGCGTGACGAGGGCTGGCACATCGACAGCTTGCGGTAAGTCATAAGGTTGTACTAATCCCCGAAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCAAGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGCGTTAAAGCAGAACTCGCGTAACCAGt                                                                                     <  2:1220753/239‑1 (MQ=255)
                                                                                                                                                           aaaGCCCGGCGGTTAACGCGGAGAAGTGGACTAATTTGCTTTGCAGCGTGTGGAGGGTTTGCGCAACGTGGCCCTGCGGTGAGGAATAGCGTTGTACAATTTCATGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa                                                                                   <  1:980556/238‑1 (MQ=255)
                                                                                                                                                                 cggcggTTTACGCCGAGAAGTCGACTAATTTTCTTTTCAGCGTGTCGAGGGCTTGCGCAACGGCGGCCTGCGGGGAGCGATAGCGATGTACCATTTTACTGATAACCGCCCGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGGGACTTGTTCCGGGTTAAAACGGGACTCGCGCAACCCGTTAAGATAg                                                                             >  2:120841/1‑239 (MQ=255)
                                                                                                                                                                  gggggTTAACGCGGAGAGGTCGACAAATTTGCTTTCCAGCGTGTGGAGGGCTTGCTCAACGTCGGCCGGCGGTGGGGTATGGCGATGTACATTTTCACGGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGGTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  1:414600/236‑1 (MQ=255)
                                                                                                                                                                  ggcgtTTACCGCCGAAAAGTCGACTAATTTGCTTTCCGGCGTGTCGGGGGCTTGCGAAGCGGGGCCCTGCGGTGAGCGATAGCGATTTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  1:448960/239‑1 (MQ=255)
                                                                                                                                                                  ggcggTTAACGCCGAGACGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCGGGGGGGAGCGATAGCGATGTCCCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  1:1287093/239‑1 (MQ=255)
                                                                                                                                                                   gcggTTACCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGTTTGCGCAACGCCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt                                                                            <  1:173858/238‑1 (MQ=255)
                                                                                                                                                                   gcggTTAACGCCGGGAAGTCGACTAATTTTCTTTCCAGCGGGTCGAGGGCTTGCGCAACCTCGGCCTGCGGTGAGCGATAGCGATGGACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCCGAGGGTGACTTCACGCCCCGGGCCGCTTAAACGAATATTCCGCTTGCCATTATCGTTTGACACCGGGACTTGTTCCGGGGTAAAACGGGAATCGCGGCACCAGTTAAGATAATc                                                                           >  2:1256634/1‑239 (MQ=255)
                                                                                                                                                                   gcggTTAAAGCCGCGAAGTCGCCTAAATTTCTTTCCAGCGGGTCGAGGGGTTGCGCAACGCCGGCCCGCGGTGAGCGATAGCGATGTACCATTTCAATGATAACCGCCAGCAAAGGAAATTCCCCCAGGACGCCTTCACGCCCCGGGCCGCTTAAACGAACATCCCACTTGCCATTATCCTTGGGCCCGGTGACTTTTTTCTGGGTAAAAGCGCACCCGCGTAACCCGTTTAGATa                                                                              >  2:1074776/1‑236 (MQ=255)
                                                                                                                                                                         tatggCCGAGAAGCCGATTATTTGCCTTTCCAGCGGGTCGGGGGTTGGCCAACAGTCGGCTGGCGGGGAGGCAGAGCGATGTCCCATTTCACTTATAGCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt                                                                      <  2:373708/235‑1 (MQ=255)
                                                                                                                                                                                 agaAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa                                                              >  2:335453/1‑239 (MQ=255)
                                                                                                                                                                                      tCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcag                                                         >  1:406275/1‑239 (MQ=255)
                                                                                                                                                                                             aTTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                                  >  1:305231/1‑239 (MQ=255)
                                                                                                                                                                                             aTTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaa                                                  >  1:197312/1‑239 (MQ=255)
                                                                                                                                                                                                tGCTTTCCAGCGTGTCGAGGGCTTGCGCACCGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc                                               <  1:1276399/239‑1 (MQ=255)
                                                                                                                                                                                                   tttCCAGCGTGTCGAGGGCTTGCGCACCGTCGGCCTGCGGTGAGCGATGGAGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa                                             <  1:195995/238‑1 (MQ=255)
                                                                                                                                                                                                   tttCCACGGTGTCGAGGGCTTGCGCAACGTCGGCCGGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa                                             <  1:998206/238‑1 (MQ=255)
                                                                                                                                                                                                   ttccaaGCGTGTGGGGGGTTTGCGCAACGTCGGCCTGGGGTGAGCGATAGCGATTTACGATTTCCCTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAc                                            <  2:1178273/234‑1 (MQ=255)
                                                                                                                                                                                                       cAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat                                        >  2:1109229/1‑239 (MQ=255)
                                                                                                                                                                                                       aagggtgtggtgcgtttGCGAAGCGGCGGCCAGCGGTGAGCAATAGCGATGTCCAATGTCGCGGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat                                        <  2:1111395/224‑1 (MQ=255)
                                                                                                                                                                                                          cGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCa                                                                                       >  2:1102492/1‑189 (MQ=255)
                                                                                                                                                                                                          cGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCAATAGCGATGTACCATTTCACTAATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCa                                                                                       <  1:1102492/189‑1 (MQ=255)
                                                                                                                                                                                                               cGAGGGTTCGCGCAACGCGGGCTGTCGGGGAGCAATAGCAATTTCCCATTTCACTGATACCCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtcatca                                <  2:214580/239‑1 (MQ=255)
                                                                                                                                                                                                                      ttGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCa                         >  1:183358/1‑239 (MQ=255)
                                                                                                                                                                                                                       tGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  2:371897/1‑239 (MQ=255)
                                                                                                                                                                                                                        gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  2:468883/1‑238 (MQ=255)
                                                                                                                                                                                                                        gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  1:34529/1‑238 (MQ=255)
                                                                                                                                                                                                                        gcgcAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg                        >  1:387831/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cTGGGGTGGGCGAGAGCAATGTACCATTCCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgctgc           <  1:668126/238‑1 (MQ=255)
                                                                                                                                                                                                                                       cTGCGGTGAGCGATAGGGATTTCCCATTTCACTGAAAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa          <  1:967658/239‑1 (MQ=255)
                                                                                                                                                                                                                                             gTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCCTCg       >  2:1247007/1‑237 (MQ=255)
                                                                                                                                                                                                                                                agcgatagcgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGa  >  1:494754/1‑239 (MQ=255)
                                                                                                                                                                                                                                                agcgatagcgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGa  >  2:605361/1‑239 (MQ=255)
                                                                                                                                                                                                                                                agcgatagcgatGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGa  >  2:565973/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  
CCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCGCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGA  >  NC_000913/989711‑990183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: