Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,040,088 A→T 15.5% Y204F (TAT→TTT)  yedY → membrane‑anchored, periplasmic TMAO, DMSO reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,040,0880AT15.5% 133.8 / 2.8 61Y204F (TAT→TTT) yedYmembrane‑anchored, periplasmic TMAO, DMSO reductase
Reads supporting (aligned to +/- strand):  ref base A (13/38);  new base T (8/2);  total (21/40)
Fisher's exact test for biased strand distribution p-value = 1.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCG  >  NC_000913/2039851‑2040326
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
ccttCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTa                                                                                                                                                                                                                                                     <  2:450392/235‑1 (MQ=255)
    cGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTTGGCGGATAAATCCTTTTGttg                                                                                                                                                                                                                                                >  1:1086829/1‑237 (MQ=255)
    cGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATCCTTATGTCg                                                                                                                                                                                                                                                >  1:737885/1‑239 (MQ=255)
    cGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCg                                                                                                                                                                                                                                                <  2:396987/239‑1 (MQ=255)
          tgatgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGACGGTGGGCTGAAATATCCTTATGTCGAAGGAt                                                                                                                                                                                                                                          <  1:275636/239‑1 (MQ=255)
           gatgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTTGGGTGGAATATTCTTATGTTGAAGGAtg                                                                                                                                                                                                                                         >  2:215140/1‑238 (MQ=255)
           gatgaCTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCGGGAATATCCTTATATCgcgagatt                                                                                                                                                                                                                                         >  2:761375/1‑232 (MQ=255)
                cTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGt                                                                                                                                                                                                                                     <  2:274756/238‑1 (MQ=255)
                    aCCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcga                                                                                                                                                                                                                                <  2:323413/239‑1 (MQ=255)
                    aCCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcga                                                                                                                                                                                                                                <  1:570618/239‑1 (MQ=255)
                    aCCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTcga                                                                                                                                                                                                                                <  1:538320/239‑1 (MQ=255)
                           gCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGCGAAATAATCTTTATTTCGAGGGagtaggctctcacgaaca                                                                                                                                                                                                                          >  2:1138521/1‑222 (MQ=255)
                              tCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGAAAAATCCTTATGTTGAAGGGGGGCGTCGCGACGAAGAAATg                                                                                                                                                                                                                      >  2:1017900/1‑239 (MQ=255)
                                ccGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                                                                                                                                                    <  2:753300/239‑1 (MQ=255)
                                acGCTGGGAGAGCGTATTTAGCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTTGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCa                                                                                                                                                                                                                    <  2:9269/238‑1 (MQ=255)
                                     ggAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGc                                                                                                                                                                                                               <  1:792976/239‑1 (MQ=255)
                                     ggAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGc                                                                                                                                                                                                               <  1:1019841/239‑1 (MQ=255)
                                              tATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGGAATATCCTTATTTCGAAGGGTTGCGTCTCGACGAAACAATGCATCCGCCCACACtca                                                                                                                                                                                                      >  1:11839/1‑237 (MQ=255)
                                               aTTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtgat                                                                                                                                                                                                     <  1:703671/239‑1 (MQ=255)
                                                  tATCGTATGCGCTTCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGTCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGAc                                                                                                                                                                                                  <  1:1001118/239‑1 (MQ=255)
                                                    tCGTATGCGCTGCGTGGAAGCGTGGGCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATAGCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCg                                                                                                                                                                                                <  1:580274/239‑1 (MQ=255)
                                                     cGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGGAAAATTCTTTTTGTGAAGGAGTGCGGCTCGGCGGAGGAAAGGAACCGCTCACCACGATGACCgg                                                                                                                                                                                               >  1:784036/1‑238 (MQ=255)
                                                       tATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAg                                                                                                                                                                                             <  2:1324302/239‑1 (MQ=255)
                                                       gaTGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAg                                                                                                                                                                                             <  1:1145028/238‑1 (MQ=255)
                                                        aTGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAg                                                                                                                                                                                             <  2:892395/238‑1 (MQ=255)
                                                         tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATCCTTATTTCGAAGGAATGGGTTTCGCCGCAGCAATGCATCCCCCCACCCTGATGAcctggggg                                                                                                                                                                                           >  1:1184182/1‑233 (MQ=255)
                                                         tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGAAATATCCTTATTTCGAGGGATTGCGGGTCGACGAGACAATGCATCCGCCCACACTGCTGAccttgggg                                                                                                                                                                                           >  2:90177/1‑233 (MQ=255)
                                                         tGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGATTTATCGGCGGTGGGCGGAAAAATCCGTATGTATAAGAGTGTGGTGTCGACGGCACAAGGCGTCCGCTACCACTAGTGACCGTCggg                                                                                                                                                                                           >  2:547229/1‑238 (MQ=255)
                                                                   ggaatccggttCGCTGGTTGTGCCGTGGATTGGTTTTCCGCTGCACAAACTGCTGTCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACGATTTATGCACCGGAACAGATGCCAGTCCAGCGGGACCGCTTTATCGGCGAGGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGATCACACTGATGACCGTAGTTGTTTATGGc                                                                                                                                                                                 <  2:786202/228‑1 (MQ=255)
                                                                                       tgCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATACTTATTTTGAAGGATTTCGTTCCGCCGCAGCAATGCATCCCCTCACACTGATGACCGTAGGGGTTTATGGGAAgggggtatcccccccaaaa                                                                                                                                                            >  1:844572/1‑222 (MQ=255)
                                                                                       tgCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGAAAAATACTTATGTGGACGGAGTGCGGCTCGGCGGAGCAATGTAATCCCCCCCCCTGGTGACCCGAGGTGGTTTTTGACAAGGCTTACACCCACaaaag                                                                                                                                                          >  2:1211226/1‑238 (MQ=255)
                                                                                       tgCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGGAATATCCTTTTTTCGAAGGATTGCGTCTCGACGCAGCAATGCATCTCCCCCCACCGCTTACCGTGGGGGTTTTTTGCAAGGCGTTTCCCCCCCAAAAt                                                                                                                                                          >  1:345328/1‑239 (MQ=255)
                                                                                                             gCACAAATTGCTGGCCCTTGCCAACCCTACCAGAAATGCAAAGTATGTCGTTTTCGAAACAATTTATGCCCCGAAACAGATGCCAGGCCACCAGGACCGTTTTATCGGCGGTGGGTTGAAATACCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCACCCGCTCACACTGATGACCGTAGGTGTTTAGGGAAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATtg                                                                                                                                    <  2:784036/239‑1 (MQ=255)
                                                                                                               acaATTTGGGGGCGCTTGCCGACCCCAACAGCAATGCGAGGTGTGTCGCTTACGAAACAATTTATGCACGGGAACAGAGGCCGGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGGCGAAGGATTGCGTCTCGACGAAGAAATGCATCCCCTCACACGGATGACCGGAGGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATtgtg                                                                                                                                  <  2:1095411/239‑1 (MQ=255)
                                                                                                                   atggCTGGCGTTTCCCGAACCCACAAAAAATGCGAAGTATGTCGCTTTAGAAACAATTTATGCACGGAAACAGATGCCGGGCCAGCAGGCCCGTTTTATGGGCGGTGGGCTGAAATTTCTTTTTGTCGAAGGATTGCGTCTCGAGGAAGCAATGCACCCGCTCAAACGGAGGACCGTAGGTTTTTAGGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGt                                                                                                                             <  2:844572/236‑1 (MQ=255)
                                                                                                                        tGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATCCTTTTGTCGAAGGGTTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGGGTTTTTGGCAAAGGGTTACCGCCACAAAATAGGGCGCCGGGGCGACTGATTGTGGCGGGGgaa                                                                                                                        >  1:450949/1‑239 (MQ=255)
                                                                                                                           cgtttGCCGAACTTCCACGCAAGGCGAATTTTGTCGCTTTCGAAACAATTTAGGCAACGGAACAGATGCCTGGCCAGCAGGACCGGTTTACCGGGGGGGGGTTGAAATATCTTTTTGTGGAGGGGTTGCGTCGCGAGGAAGCAATCCCCCCGCACTCTCGGATTGCTGTAGGGTTTTTTGGGAAGTCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAata                                                                                                                      <  2:315636/235‑1 (MQ=255)
                                                                                                                                         cccAGCAAGGGGAGGTGTGTCGCTTTCGAAACAATTTATGCCCCGGAACAGATGCCAGGCCAGCAGGACCGTTTTACCGGGGGGGGGCTGAAATTTCCTTATGGCGAAGGATTGCGTCTCGACAAAGCAATGCACCCGCTCACACTGATGACCGTAGGTTTTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGAt                                                                                                       <  2:1184182/238‑1 (MQ=255)
                                                                                                                                                aggcgaatgtggTCGCTTTGGAAACAATTTAGGCACCGGAACAGATGCCAGGCCAGGGGGACCGTTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAGGGTTTGCGTCACGCCGAAGCAATGCATCCGCTCACACTGTTGACCTTAGGTTTTTATGGTAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg                                                                                                <  2:1086829/228‑1 (MQ=255)
                                                                                                                                                aggCGATGTAGGTCGCTTTCGAAAAAATTTATGCACGGGAGAAGATGCGAGGCCGGGAGGACCGGTTTACGGGGGGTGGGCTGAAATATCCTTATGTCGAGGGGTTGCGTCGGGACGAAGCAAGGCATCCGCTCACACTGATGACGGTAGGTGTTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg                                                                                                <  2:132743/237‑1 (MQ=255)
                                                                                                                                                   cGAGGTATGCCGCTTTCGAAAAAATTTATGCACGGGAACAGATGCCAGGCCAGCAGGACCGCTTTATGGGCGGTGGGCAGAAATTTCCTTAGGGCGAAGGATGGCGTCCCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGa                                                                                               <  2:639617/237‑1 (MQ=255)
                                                                                                                                                     aagttggTCGTTTTCAAAACAATTTATCACCCGAAAAAGAGGCCGGGCCAGCAGGACCGCTTTATCGGCGTTGGGATAAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGTTGCCCGTGGGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  1:547229/233‑1 (MQ=255)
                                                                                                                                                     aaGTATGTCGCTTTGAAAAAAATTGTGGCACAGGAACAGAGGCCGGGCGAGCGGGACCGCTTTACGGGGGGGGGGGTAAAATCTCCTTATGGAAAGGGATTGCGTCTCGACGAAGCAATGTACCCGCCCACAGTGGGGACGGTAGTTGTTTATGGAAAGGGGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  2:737885/239‑1 (MQ=255)
                                                                                                                                                     aaGTATGTCGCTTTAGAACAAATTTATGCCCCGGAACAGATGCCAGGCCAGAGGGCCCGCTTTACCGGCGGTGGGTGAAAATATCCTTATGTGGAAGGTTTGCGTCTCGACGAAGAAATCCACCCGCCCACACTGATGACCGTGGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  2:450949/239‑1 (MQ=255)
                                                                                                                                                      agttagTCGCTTTCGAAACAATTTATGCACCGGAACAGATCCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAGGGTTTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAGGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  1:927976/233‑1 (MQ=255)
                                                                                                                                                      agtagttCGCTTTCGAAAAAATTTATGCCCCGGAACAGATGCCAGGCCAGCAGGACCGTTTTGGCGGCGGGGGGCAGAAATATCCTTATGTCGAGGGATTGCGTCTCGAGGAGGGAATGCACCCGCTCACACTGATGACGGTGGGTTTTTAGGGCAAGGGGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  2:345328/232‑1 (MQ=255)
                                                                                                                                                      agtagtgcggtttCGAAACATTTTATGCCCCGGAACAGATGCCAGCCCAACAGGACCTCTTTATCGGGGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCCCACACTGATGACCGTAGGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  2:711693/228‑1 (MQ=255)
                                                                                                                                                      aGTATGTCTTTTACGAAATAATTTGTGCCCCGGAACAGATGCCAGGCCAGCAGGACCGCTTTAGCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGAGGAAGCAATCCTTCCGCTCACTCTGATGCCCGTAGGTGTTTTGGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  1:1011715/238‑1 (MQ=255)
                                                                                                                                                      aGTAGGTCGCTTTCAAAACAATTTATGCACCGAAACAGATGCCAGGCCAGCGGGCCCGCTTTAGCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCCCGACGAAACAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  1:1016017/238‑1 (MQ=255)
                                                                                                                                                        tttGTCGCTTTCGAACCAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGCCCGCTTTATCGGCGGTGGGTTGAAATATCCTTATGTCGAAGGTTTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAGGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTaa                                                                                                             <  1:636790/216‑1 (MQ=255)
                                                                                                                                                             cGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATCCTTTTTTCGAAGGATTGGGGTTCCGCGAAGCAAAGGATCCGCCCCCACTGGTGACCCGAGGGGTTTATGGGCAGGCGGTTCCCCCCCCAAAAAGGGGCGCCGTGGGGCGGTTTGTGGCGGGGGAGAATGGCTTTAAAGGGGTGAAAAAGATaattaggatag                                                                                   >  2:936054/1‑229 (MQ=255)
                                                                                                                                                                tttCGAAACAATGTATGCACAGGAACAGAGGCCAGGCCAGAAGGACCGTGTTATCGGGGGTGGGCTAAAATATCCTTATGTCGAAGGATTACGTCTCGACGAAGCAAGACATCCGCTAACACTGATGACCGAAGTTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGc                                                                               <  1:311437/239‑1 (MQ=255)
                                                                                                                                                                  tCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATCCTTTTGTTTAAGGGGTGCGTCTCGGCGCAACAAAGCATCCGCTCACCCTGCTGTACGGAGGGGTTTTTTGCCAGGCGGTACCCGCCCCAAAAAGCGCGCCGGGGCGGCGGCTTTTGCGGCGGGAATAAGGCTTTTAAAGGGTTAAAtttttcttttttacacaaaca                                                                              >  2:636790/1‑219 (MQ=255)
                                                                                                                                                                           tttCTGCACCGGAACAGACGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAGGGATTGCGTCTCGACGAACAAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAg                                                                    <  1:1211226/239‑1 (MQ=255)
                                                                                                                                                                                  cccGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCcg                                                                        <  1:397845/227‑1 (MQ=255)
                                                                                                                                                                                  aCCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGGGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGAAATGCATCCGCTCACCATGATGACCGTAGGTGTTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt                                                                                           <  1:1121032/210‑1 (MQ=255)
                                                                                                                                                                                       aaCAGATGCCAGGCCAGCAGGACCGTTTTATCGGCGGGGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGAGGCCCGTGGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAac                                                         <  1:1221113/238‑1 (MQ=255)
                                                                                                                                                                                            aTGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTTTTTTGGAGGATTTAGGCTCGCCGAAGAAATACAACCACTCACCCCGTTGACCGCAGGGGGTTTTGGCAAAGGGGTTCCCCCACAAAAAAGGGCGCCCGGGGGGGTGCGTGTGCCGGGGGAAAATTTGTTTTTAAGGGTTTAAAACATAATCAGGATTTAACCTGACCcccgggggccgccccaccaacg                                                    >  2:771862/1‑221 (MQ=255)
                                                                                                                                                                                            aTGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGGGGAAAATTCTTTTGTTGAAGGGATGCGGCTCGACGAAGGAATGCATCCCCCCCCCCTTATGACCCGAGGGGGTTTTTGCAAGGGGTTAACCCCCCAAAAAAGGGGGGCGGGGGGGCTGATTGTGCCGGGGAAAAAAGGCTTTTAAGGGGGTAAAACGAAAAGCCATATTTAGCTGAccccccgggggccgcccgcttcttt                                                   >  2:397845/1‑217 (MQ=255)
                                                                                                                                                                                                                                 taaattatCCTTAGGTGGAGGGTTTGGTTCTGGAGGAAGCATTGCTCCCGCCCCACCGGATGACGGTGGGGTTTTAGGGCAGGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGc               <  2:11839/233‑1 (MQ=255)
                                                                                                                                                                                                                                     atatCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGACTACGGTTTTTACGCCAACg          >  1:841227/1‑239 (MQ=255)
                                                                                                                                                                                                                                         tCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTa       >  2:1132457/1‑239 (MQ=255)
                                                                                                                                                                                                                                              aTGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCg  >  2:711159/1‑239 (MQ=255)
                                                                                                                                                                                                                                              aTGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCg  >  1:391960/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                   
CCCTCGATCATGATGACTTAACCCGTCGCTTCCCGCTGGAAGAGCGTATTTATCGTATGCGCTGCGTGGAAGCGTGGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCG  >  NC_000913/2039851‑2040326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: