Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,228,270 A→C 26.6% pseudogene (338/1287 nt) mdtQ ← pseudogene, putative channel/filament proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,228,2700AC26.6% 108.5 / 9.1 68pseudogene (338/1287 nt)mdtQpseudogene, putative channel/filament proteins
Reads supporting (aligned to +/- strand):  ref base A (41/6);  new base C (3/18);  total (44/24)
Fisher's exact test for biased strand distribution p-value = 9.71e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGT  >  NC_000913/2228039‑2228503
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
gCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCGTTCCCCCCCCCGGGGCTTAACCCGTCCCCAGCCGGGAGTAACccccc                                                                                                                                                                                                                                               >  1:248805/1‑237 (MQ=255)
     aaTATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCCCGGTCCCCCCCCCCGGCCTTAACCCTACCCCGGGGGGGAGTAAACTCCTCCCCAt                                                                                                                                                                                                                                        >  1:544568/1‑239 (MQ=255)
          cGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGCTTTCTCGCGTTCCCCCGCCCGCGCTTTTACCCTTCCCCGGCGGGCCGCAAACTCCCCCCGGTTCttt                                                                                                                                                                                                                                  >  1:105349/1‑239 (MQ=255)
          cGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTAAGCTGCCAGCCAGCAATTGGCGGGCTTTCTCGCGGTCCCCCGCCCCTGCTTTTAACGCACACCGGGTGGCAGTAAACCCCCCCCCACTCttt                                                                                                                                                                                                                                  >  2:779984/1‑239 (MQ=255)
            gTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGTTCGCGCTCCGCCGCCCCTGCTTTAAACGTACCCAAGCGGGCCGTAACCTCCGCCCCAGTCtttt                                                                                                                                                                                                                                 >  1:393417/1‑237 (MQ=255)
            gTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCt                                                                                                                                                                                                                                                  <  1:1097723/223‑1 (MQ=255)
              ttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTcccc                                                                                                                                                                                                                              <  1:1067846/239‑1 (MQ=255)
              ttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTccc                                                                                                                                                                                                                               <  1:1269034/238‑1 (MQ=255)
              ttCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTccc                                                                                                                                                                                                                               <  2:316400/238‑1 (MQ=255)
              tgccACACCTTCAACTGAAGAAGTAATCCCGGTCTGATAGAGCTGGCGATCGGTCGCGATAATGGTGGTCTGCTCTTTTGCTATTTGCTTCAAGACCGTGTTTACCGACGCCTGGGTTTGCCACACCCAGTTCAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTGcccc                                                                                                                                                                                                                              <  2:218677/237‑1 (MQ=255)
                 cacaCCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGTCGGGGTTGCTCGCGGTCCCCCGCCCCGGCTTTAACCTCACCCACGGGGGGAGCTACATCCGCCCGGTTCTTTCCCCa                                                                                                                                                                                                                             >  2:161552/1‑237 (MQ=255)
                   cacCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCCCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGGAAACTCCGCCCGATTCTTTCCCCAGa                                                                                                                                                                                                                           >  1:1117300/1‑237 (MQ=255)
                      cTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTTCGCCGCCCGCGCCTTTAACCCACCCAGGGGGGGCGTAAACCCCCCCCgcgttctgttctttataca                                                                                                                                                                                                                         >  2:1097723/1‑220 (MQ=255)
                         cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACTCTGCCAGCCAGCCATTGGCGGGGTTTGTCCCGGTCCGGCGCCCCGTCCTTTAACCGGCCCCGGCGGGGGACAAACCCCCGCCGCTTTCTTTccccctcactgc                                                                                                                                                                                                                      >  2:751771/1‑229 (MQ=255)
                         cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCCGGCGGGCAGTAACCCCCGCCCCGTTTTTTCCCCCGATATCgaga                                                                                                                                                                                                                  >  1:1261303/1‑239 (MQ=255)
                         cAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCCGCGGTTCCGCCCCCCCTGCTTTTACCGCACCCCCGGGGGGCACGACCCCCCCCCCGATCTTTCCCCCAGaattgtga                                                                                                                                                                                                                    >  2:588789/1‑232 (MQ=255)
                          aaCTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAGTGGAGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGATCGGCGTTCCCCGCCCCCTCTTTTACCATACCCCGGCGGGGCGTTACCCCCCGCCCCATCTTTTccccgcaatcgcgat                                                                                                                                                                                                                   >  2:510482/1‑228 (MQ=255)
                                agaagTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGGTCCCCCCCCCGTGCTTTAACCGTACCCAGGCGGGCCGTAACCTCCGCCCGATTCTTTCCCCCGATATCGAGATGCCAg                                                                                                                                                                                                           >  1:556770/1‑238 (MQ=255)
                                    gTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCTCGCGTTCCGCCCCCCGTGCTTTAACCGCACCCATGCGGGCCGTAACCTCCGCCCCCTTCTTTTCCCCGAGATCGTGATGCCAGCccg                                                                                                                                                                                                       >  1:1159706/1‑238 (MQ=255)
                                    gTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCCGTTCGCCGCCCCGGCTTTAACCCCACCCCGGCGGGGAGTAACCTCCCCCCCCGTCTTTTCCCACATATCCAGATGCCAGcccc                                                                                                                                                                                                       >  2:1233532/1‑237 (MQ=255)
                                            gTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCCCCGCCCGGGCTTTTACCGTACCCAGGCGGGCAGTAACCTCCCCCCGATTTTTTCCCCAGATATCGAGATGCCCGCCCGCCGTTTAg                                                                                                                                                                                              >  1:615416/1‑238 (MQ=255)
                                             ttCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTGTCGCGCTTCGCCCCCCGTGCTTTTACCCGTCCCCAGCGGGGCAGGCACTCCGCCCGGCTCTTTTCCCCATATTCGAGATGGTCGCCCGCCGTTatac                                                                                                                                                                                             >  2:628952/1‑235 (MQ=255)
                                             ttCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCTCGCGGTCCGCCGCCCGGGCTTTTACCCTACCCCGGGGGGGCATAAACTCCCCCCCGTTCTTTTCCCCCATATCGGGGTTGCAGCCCCCCGTTACGc                                                                                                                                                                                             >  2:1309124/1‑238 (MQ=255)
                                             ttCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCCCGTTCCGCCCCCCGGGCTTTAACCCTACCCACGGGGGCCGGAACCCTCCCCCGGTTCTTTTCCCCCATATTGTGAATGCAAGCCCCCCGTTAAGc                                                                                                                                                                                             >  1:4625/1‑239 (MQ=255)
                                                      aGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCCCCGCCCGTGCTTTTACCGTACCCAGGCGGGCAGTAACCTCCCCCCGATTCTTTCCCCCGATATCGAGATGCCAGCCCGCCGTTAAGCCCAAAATAc                                                                                                                                                                                    >  1:86242/1‑238 (MQ=255)
                                                            cGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCCTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTTCCCCGATATCGAGATGCCAGCCCCCCGTTAAGCCAAAAGTACCGTTGgt                                                                                                                                                                            >  1:1286388/1‑239 (MQ=255)
                                                             gATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTACCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGgtg                                                                                                                                                                           <  1:241165/239‑1 (MQ=255)
                                                                       gcaagtGGTGTTCGCCGCTTTTTCTATTGCCCGAACGCCCGTGTTAGACCCCGCCTGGGTTGCCCCCCCCCAGTACGGGCGGGCTGCCCGGCCAGCCAGCATTTGGCGGGTTTGCGCGCGTTCCGCCCCCCGTGCTTTACCCGTACCCAGGCGGGCGGTACCCTCCGCCCGATTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACggg                                                                                                                                                                <  2:1002013/233‑1 (MQ=255)
                                                                       gATAATGTTGTCCTCCTTTTTTTCTTTTTGCTGCAAGACCGTTTTTAACGCCGCCGGGGTTGGCCCCCCCCAGTACGGGCGGGCTACGCTGCCAGCCAGCAATGGGGGGGTTTGCTCGCTTTCCGCCGCCGGTGCTTTAACCGTCCCCAGGCGGGCAGTACCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACggg                                                                                                                                                                <  2:1261303/238‑1 (MQ=255)
                                                                             ggTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACCCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGTTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTCCCCAGGCGGGCAGTACCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCg                                                                                                                                                        <  1:1130764/239‑1 (MQ=255)
                                                                                 ttCTGCTTTTTTTCTATTGTCGGCAAGCCCGTGTTTACCGCCGCCGGGGTTCGCCACCCCCAGTACAGGCGGGCTACGCTCCCGCCCAGCAATGGGGGGGTTTGCTCGCGTCCCGCCGCCCGTTCTTTACCCGTCCCCAGGGGGGCAGTACCCTCCCCCCGATTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTAcc                                                                                                                                                    <  2:615416/239‑1 (MQ=255)
                                                                                   cTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCCGTAACCTCCGCCCGATTCTTTTCCCCGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCt                                                                                                                                                   >  1:860516/1‑238 (MQ=255)
                                                                                           tttgcgtttGCTGAAAGCCGTTTTTACCCGCCGCCGGGGTTGGCCACGCCCACACACGGGGCGTTACGCTGCCAGCAGGCAGTGGTGGGTTTGGCTCGTGTCCCGCGGCCGATGCTTGACCAGGACCATGGCGGGAGTGACCCTCCGCCCACTCTTTTCCCCGAATACCGTGAGTCCTGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGa                                                                                                                                          <  2:763338/233‑1 (MQ=255)
                                                                                           tttCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCTCGCGTTCCGCCGCCCGCGCTTTTACCGTACCCAGGCGGGGCGTAACCCCCGCCCGCTTCTTTCCCCCGATATCCAGATGCCCACCCGCCGGTAAGCCCAAAGTAACCCTGGTGTACCACCGGGCGGCCGTACCCTCGGccacg                                                                                                                                          >  2:335867/1‑236 (MQ=255)
                                                                                                aTTGGCTGAAAGACCGTTTTACACGCCGCCTGGGTGCGCCACCCCCAGTACGGGCGGGCTACCCTGCCCGCCAGCAATGGGCGGGTTTGCTCGCGTCCCGCCCCCCGTGCTTTAACCGTCCCCGGGCGGGCAGTACCCCCCGCCCGATTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGtt                                                                                                                                     <  2:849228/239‑1 (MQ=255)
                                                                                                   tgctgcAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTTCCAGCCCGCAATTGGCGGGGTTTCTCGCCTTCCGCCCCCCCGGCTTTAACCCTACCCCGGCGGGCCGGAACCTCCCCCCCCTTCTTTTCCCCGATATCTCGATGCCCGCCCCCCCCTAAACCCAAAGTAACGCTTGGGTGCCCCCGGCCCGCCCTACCCGCGCCCCGCTCGTTCac                                                                                                                                  >  2:1185461/1‑238 (MQ=255)
                                                                                                   tgctgcAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCTCGCGGTCCGCCGCCCGTGCTTTTAACGTACCCAGGCGGGCCGGAACCCCCCCCCCCTTCTTTTCCCCCAAATCCAGATGCCCGCCCCCCGGTTAACCAAAAGTACCCCTTGTGGACCCCCGGCCGGGGGGACCTCCgggccgggcgtttag                                                                                                                                  >  2:1207028/1‑226 (MQ=255)
                                                                                                           gACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTGTCCCGTTCCCCCGCCCCTGCTTTAACCCTAACCCGGCGGGGCGTAACCTCCCCCCGCTTCTTTTCCCAGATATCCAGATACCCGCCCCCCGTTAAGCCCAAAAATCCCTTGGTGGACCCCCGGCCGGTCGGAACTGCGGCCGGAGCGGTCAGAGCAAACg                                                                                                                         >  2:372653/1‑239 (MQ=255)
                                                                                                             ccGTGTTTAACGCCGCCGGGTTTTGCCACCCCCAGTACGGGCGGGCTCCGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTACCCGTCCCCAGGCGGGCAGTACCCTCCGCCCGATTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGc                                                                                                                       <  1:682587/239‑1 (MQ=255)
                                                                                                                tgtTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGGTCCCCCCCCCCCGGTTTTAACCTACCCCAGCGGGGAGTAACCTCCCCCCCCTTCTTTTCCCCGATAACCAGATTCCCGGCCCCCCCTTAGCCCAAAATACCCGTGGGGGACCCAGGGCCCGGCCTACccgccgccgcggtgttcctgggaaacggccc                                                                                                                      >  2:6426/1‑216 (MQ=255)
                                                                                                                tgtTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGGCCCCGGTCCCCCGCCCCGGCCTTATACGCACCCCGGCGGGCCGGAACCCCCCCCCCGATCTTTCTCCAGATATCCCGATGGCCCCCCCCCTCTAAACCAAAAGTACCCTTGGTGTGCCACAGGCCGGGCGTCACCGTCGCCGGGTAGTTCCAAGCCAAACGGcc                                                                                                                      >  2:180379/1‑238 (MQ=255)
                                                                                                                        cgccgcCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCTCGCGGTCCCCCCCCCCTGGTTTAACCCTACCCCGGCCGGCAGGAACCTCCCCCCCCTTCTTTTCCCCCATATCCTGATGGCCACCCCCCCGTTAACCCAAAATACCGTTTGGTTACCCACGGCCGGTCGGTCCCCGCGCCGGGTCGTTCACGGCACACAGcccccctaaa                                                                                                              >  2:671070/1‑232 (MQ=255)
                                                                                                                             ccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTGTCCCCGTCCGCCCCCCCGGGCTTAACCCGAACCCCGGCGGGAGTGACCTCCCCCCGCTTTCTTTCCCCCAATACCAGGTGCCCGCCCCCCCTTAAAGCCCAAATACCCTTGGTTGTACACCGGCCCGTCGTACCCTCGGCCTGGCTGCTCAGAACAAACACCCCCAATAAACCTTc                                                                                                         >  2:1303864/1‑238 (MQ=255)
                                                                                                                             ccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCCCGCGGTCCGCCGCCCGGGCTTTTACCCTACCCAGGCGGGGAGTAAACCCCGCCCGGTTCTTTCCCCCGATATCGAGATGCCAGCCCCCCCGTAAGCCAAAAATAACCTTTGGGTACCACGGGGCGGGCGTACCCCCGGCCGGAGCGTTCAGAGGCAACGGCCCCCCTTAACCTTCt                                                                                                        >  2:505640/1‑239 (MQ=255)
                                                                                                                             ccTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCCCCGCCCCCGCTTTTACCCGACCCACGCGGGGCGTAAACTCCCCCCCGTTCTTTTCCCCGATATCGAGATGCCCACCCCCCGCTAAGCCCAAAATAACGTTGGTGTAACACCGGGCCGGCGTACCCGCGGCCCGGACGGTCAGAGCAAACGGGCCCCTTTAACCTTCt                                                                                                        >  2:496678/1‑239 (MQ=255)
                                                                                                                                  ttttGCCCCCCCCATAACAGGCGGTCTACGCTGCCAGCCAGCAATTGGGGGGTTTCCTGGCGTCCCGCCCCCCGTGCTTTAACCGTCCCCGGGGGGGAAGTACCCTCCCCCCGATTCTTCCCCCAGATATCGAGAGGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCg                                                                                                    <  2:544568/237‑1 (MQ=255)
                                                                                                                                   gtttcccccccccgtgcggggCGGGCTCCGCGCCCCGCCAGCATTTGGCGGGTTTGCCCGCTTCCCCCCGCCCGTTTTTTACCCGTCCCCAGGCGGGCGTTACCCCCCGCCCGATTTTTCCCCCAGATAGCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCg                                                                                                    <  2:1315979/219‑1 (MQ=255)
                                                                                                                                       ccACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCTCCCGTTCCGCCGCCCGTGCTTTAACCCTACCCAGGCGGGCAGTAAACTCCCCCCGATTCTTTCCCCCGATATCGAGATGCCCACCCGCCCTTAAACCCAAAGTACCCTTTGGGTACCCCCGGGCCGGCGGACCTGCGGCCGGGTCGTTCCGAGCAACAGGCCCCACTAAGCCTTCTGCCGCCAttt                                                                                              >  2:230716/1‑239 (MQ=255)
                                                                                                                                            cccAGTACAGGCGGTCTCCGCTGCAGGCCAGAAATTGGCGGGTTTCCTCGTGTTCCCCCGCCCGGTTTTAAACCGTCCCCAGGGGGGCAGTACCCTCCCCCCGATTTTTCCCCCAGATAGCGAGAGGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGc                                                                                         <  2:1267132/239‑1 (MQ=255)
                                                                                                                                                       gCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTGTCGCGGTTCGCCGCCCGTGCGTTTACCGTCCCCAGGCCGGGAGTAACCTCCCCCCGCTTTTTTTCCCACGGATCGAGATGCCCGCCCCCCGTTAAACCCAAAATAACGTTGGGGGTCCACGGGCCGGGCGTGCGCGCGGCCCGGGCGGTCACGGCCAACGGGCCCCCTAACAACTCTCTCGACCTTTTTTGCTGCCCCatt                                                                                >  2:168018/1‑237 (MQ=255)
                                                                                                                                                       gCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCGGTTCGCCGCCCCTGCTTTTACCGTACCCCCGCGGGGAGTAACCTCCGCCCCATTCTTTTCCCCGGTATCCCGAAGCCAACCCGCCGTTAAGCCAAAAGGACCGCTGGTGTACCACGGGCCGGGCGTGCCTGCGGCCCGGTCCGGCTGAGCCAACTGCCCCCCTAAGCCTCCTCCCGACCTTTTTTTCCGCTccctc                                                                                >  2:1023423/1‑235 (MQ=255)
                                                                                                                                                            cTACGCTGCCAGCCAGCAATTGGCGGGGTTTCTCGCGTTTCGCCCCCCGTGCTTTTACCCCACCCAGGCGGGGAGTAACCCCCGCCCCCTTTTTTTCCCCAATATCGCGAAGCCACCCCGCCCCTAAGCCCAAAAGACCGTTGGTGTACCACGGGCCGGGCGCACCCGCGGCCGGGTCGTTTCGCGCAAAAGGGCCCCCTAAACCCTCTGCCGGCAtttttttgcccgccacatcccc                                                                           >  2:1224294/1‑222 (MQ=255)
                                                                                                                                                                       gccagcATTGGGGGGGTTGCCTCGCTTTCCGCCGCCCTTGCTTTACCCGTCCCCAGGCGGGCATTACCCCCCCCCCGATTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGAt                                                               <  1:1233532/239‑1 (MQ=255)
                                                                                                                                                                                  tgggggTTTGCCCCGCGTTCCGCCCCCCGTGCTTAAACCGTCCCCAGGGGGGAAGAACCCCCCGCCCAATTTTTCCCCCAAAAATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGa                                                        <  1:163008/234‑1 (MQ=255)
                                                                                                                                                                                                     gTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTACGCCTTCTGCCGACATTTTTTGCCGCCCCATCTCCGCCGCAAAGTCCACCTGCGGACCATCCTGACTGGcg                                         >  2:1094475/1‑235 (MQ=255)
                                                                                                                                                                                                        ccgcccCCCGTGCTTAAACCGTACCCAGGCGGGAAGTAACCCCCCCCCGATTCTTCCCCCAGATACCGAGATGCCGGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCAGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGcc                                   <  1:335867/239‑1 (MQ=255)
                                                                                                                                                                                                                       cTTTAACCGTCCCCAGGCGGGCAGTACCCCCCGCCCGATTCTTCCCCAAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCt                       <  1:505640/239‑1 (MQ=255)
                                                                                                                                                                                                                        ttttAACCGTCCCCGGGCGGGCAGTACCCCCCGCCCGATTCTTTCCCCAGATATCGAGAGGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCt                       <  1:230716/238‑1 (MQ=255)
                                                                                                                                                                                                                                gTACCCAGGCGGGCAGTACCCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCACAACTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTACAGAGCAACCGGCCCCATTAAGCCTTCCGCCGCCCTTTTTTGCCGCCCCATATCCGCCGAAAAGTCGATCTGCGGACCACCCTGAGCGGCACCTGCCTTCGCCGCGCCTTCaactc                >  2:166032/1‑237 (MQ=255)
                                                                                                                                                                                                                                gTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagcta                >  2:934381/1‑238 (MQ=255)
                                                                                                                                                                                                                                catcccaagccGGCCAGAACCCCCCGCCCGTTTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagcta                <  1:1224294/229‑1 (MQ=255)
                                                                                                                                                                                                                                 tACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagcta                >  2:741399/1‑237 (MQ=255)
                                                                                                                                                                                                                                 tACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagcta                >  1:715508/1‑237 (MQ=255)
                                                                                                                                                                                                                                  aCCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCagcta                >  2:77098/1‑236 (MQ=255)
                                                                                                                                                                                                                                     ccgggCGGGCAGTACCCGCCCCCCGATTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGa           <  1:557141/236‑1 (MQ=255)
                                                                                                                                                                                                                                         gCGGGCAGTACCCTCCGCCCGTTTCTTCCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATg        <  1:372653/238‑1 (MQ=255)
                                                                                                                                                                                                                                             ggCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATgc       >  1:278877/1‑236 (MQ=255)
                                                                                                                                                                                                                                               cAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGt  >  2:842661/1‑239 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
GCATTAATATCGGTTTCCACACCTTCAACTGAAGAAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCTCGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGAGATGCCAGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGGCCGGTCGTACCTGCGGCCGGATCGTTCAGAGCAAACGGCCCCATTAAGCCTTCTGCCGACATTTTTTGCCGCTCCATATCCGCCGAAAAGTCGATCTGCGGACCATCCTGAGTGGCAACTGCCTTCGCCTGGGCTTCAGCTAGCTGAATGCGCTGT  >  NC_000913/2228039‑2228503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: