BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,530	T→G	11.3%	V266G (GTG→GGG)	thrC →	L‑threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,530	0	T	G	11.3%	399.1 / 2.3	149	V266G (GTG→GGG)	thrC	L‑threonine synthase
Reads supporting (aligned to +/- strand): ref base T (16/106); new base G (24/2); total (41/108)
Fisher's exact test for biased strand distribution p-value = 5.73e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

TCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGA  >  NC_000913/4300‑4767

tCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTTCTGGGGGAGGCA‑C‑TCGGTCTGCCGGGGGAACGtt                                                                                                                                                                                                                                         >  1:1414184/1‑239 (MQ=255)

       cTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCCGCTGGCGAGGCGC‑A‑TCGGGCTGCCCGTGCAACGGtttttt                                                                                                                                                                                                                                    >  2:1400500/1‑237 (MQ=255)

         ggTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAGAGGA‑C‑CCGGGCTGCCGGGGGAACGTTTTAttgttg                                                                                                                                                                                                                                >  2:1421263/1‑239 (MQ=255)

         ggTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGCAATCA‑C‑TCGGTCTGCCGGGGAAACGTTTTAtttgt                                                                                                                                                                                                                                 >  1:237218/1‑235 (MQ=255)

          gTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCGTCTGGGGGAGCGA‑C‑CAGGGCTGCCGGGGAGACGGttttttttgga                                                                                                                                                                                                                               >  2:913578/1‑234 (MQ=255)

              aGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                           <  1:109679/239‑1 (MQ=255)

               gCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGGAGGCA‑C‑TCGGGCCTGCGGGGGAACGGTTTTTTTCTGCGGCCa                                                                                                                                                                                                                          >  2:124942/1‑239 (MQ=255)

                  ggCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTGCTTTGAAGCGGTTGCGCAGCTGCCGCAGGCGACGCGCAACCAGCTGGTTGTCACGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTATCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  2:95596/238‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  2:281742/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:913578/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:1100182/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  2:1113839/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  1:764167/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                        <  2:1207342/236‑1 (MQ=255)

                    cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGCCTGCTGGCGAAGTCA‑C‑TCGGTCCGCCGGGGGAACGTTTTATTGCTGCGACCAACgcg                                                                                                                                                                                                                     >  1:951894/1‑237 (MQ=255)

                        tgatgatgaAGAGCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                 <  2:1025045/239‑1 (MQ=255)

                        tgatgatgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                 <  1:1377571/239‑1 (MQ=255)

                          atgatgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCGGGCGCAGTCA‑C‑TCCCTCTGCCGGGGGAAAGGTTTATTGCTGGGGCCACCGTTAACggg                                                                                                                                                                                                               >  2:714427/1‑237 (MQ=255)

                              tgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCCTGCGACCGCA‑C‑TCGGTCTGCCGGGGAAAAGATTTATTGTTGCGCCCCACGTGAACGaacacc                                                                                                                                                                                                           >  2:206604/1‑234 (MQ=255)

                               gaagaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGt                                                                                                                                                                                                          <  1:124942/239‑1 (MQ=255)

                                 agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                        <  1:861410/239‑1 (MQ=255)

                                 agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                        <  1:710175/239‑1 (MQ=255)

                                     cTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTTTGCTGGCCAAGTCA‑CGCCGCTCT‑CCGGGGAAGCGGATTTTTGCTGCGCACAACCTGGACGATACCCTGCCAc                                                                                                                                                                                                     >  2:806726/1‑238 (MQ=255)

                                        aaaGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCCGCTTGCGCAGTCC‑C‑TCGGTCTGGCGAGGAAACGTTTTATTTCTTGGCCCCACCTGAACGa                                                                                                                                                                                                                >  1:1426505/1‑224 (MQ=255)

                                             ggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGGCA‑C‑CCGGGCTTCCCGGGAAAAGTTTTATTGGTGCGACCAACGGGAACGATAACGGGGCCCGGTTCCtt                                                                                                                                                                                             >  1:96760/1‑237 (MQ=255)

                                               cgcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGACA‑A‑TCGGTCTGCCGGGGGAACGTTTTTTTGCTGCGACCCACGTGAACGCTACCGTGCCACCTTTCCTGCa                                                                                                                                                                                           >  1:748200/1‑238 (MQ=255)

                                                gttAGGTTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTTGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  2:932390/237‑1 (MQ=255)

                                                gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  1:153346/239‑1 (MQ=255)

                                                gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  1:873507/239‑1 (MQ=255)

                                                gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  1:1022823/239‑1 (MQ=255)

                                                 cTAGGGTTAACCCCGGCTAACTCGATTACCATCAGCGGTTTGCTGGGGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                         <  2:1228447/238‑1 (MQ=255)

                                                  tAGGGTTAAACTCGGCTAACTCGATTACCATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAATGATACCGTGCCACGTTTCCTGCacga                                                                                                                                                                                        <  1:910058/238‑1 (MQ=255)

                                                       ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                   <  2:1337234/238‑1 (MQ=255)

                                                          aaCTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTg                                                                                                                                                                               <  1:315381/239‑1 (MQ=255)

                                                            cTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                                                                                                                                                                             <  1:1011034/239‑1 (MQ=255)

                                                               ggCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                   >  1:747595/1‑230 (MQ=255)

                                                                gCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCTGGCGAAGTCA‑C‑CCGGTCTTCCGGTGAAAAGTTTTTTTTCTGCGACCAACGTGAAAGATACCAGGCCCCCGTTCCTCCACCACGGTcgcgtggcaa                                                                                                                                                                          >  2:1347245/1‑229 (MQ=255)

                                                                         aTTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGGTGCTGGCGGAGTCAGC‑CCGGCCGACCGGGGAAA‑GTTTTTTTTCTTGGACCGACCTGGACGATACCATCCCCCGGTTCCTTCTCGGCCGGCAGCTGTCACCCAAAGCgg                                                                                                                                                                 >  2:178708/1‑237 (MQ=255)

                                                                          ttAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCa                                                                                                                                                                                                                          <  1:302832/180‑1 (MQ=255)

                                                                          ttAACATAAGCCGTTTGCTGGCGCAGAGTTTCGACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCACGGTGCCAAGCGGAAACGTTGGCGATTTGCCGGCGGGTCTGCTGACGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATCCCGTGCCACGTTTCCTGCTCGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               <  2:1020616/239‑1 (MQ=255)

                                                                              cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                           <  1:136076/239‑1 (MQ=255)

                                                                              cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                           <  1:545076/239‑1 (MQ=255)

                                                                              cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                           <  1:500799/239‑1 (MQ=255)

                                                                                   gCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTTTTTCTGCGACCAACGTGAACGCTACCGGGCCACGTTTCCTGCACGACGGTCAGTGGGCACCCACAGCGACCCAGGCGCCg                                                                                                                                                      >  1:576906/1‑239 (MQ=255)

                                                                                       tttGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGTTTCTGGGGGAGGCA‑C‑CCGGTCCGCCGGGGAAACGCTTTATTGCTGGGGCCAACGTGAACGATACCGTGCCCAGTTTCCCTCACGACGGGCCGTGGTCCCCCAAAGCGCTCCAGGCGACGTTAt                                                                                                                                                  >  2:748399/1‑239 (MQ=255)

                                                                                         tGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGGAGTCA‑C‑TCGGGCCGCCGGGGAAAAGGTTTATTGGTGGGACCCAAGTGAACGGTAACCTGCCCCGCTTCCTGCCCGACGGGCCGGGGGCCACCAAAACGAACCAGGGGGCGTTGTc                                                                                                                                                 >  2:1477662/1‑238 (MQ=255)

                                                                                              gcgcAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCCGGCGAAGACA‑C‑CCGGGCTGTCGGGGGAACGTTTTATTTGTGCGACCAACGGGAACGAAACCGGGCCACGCTTCCTTCCCGGCCGGCCGAGGTCACCCCCAACGACCCAGGGGGCGCTATctccag                                                                                                                                            >  2:496802/1‑233 (MQ=255)

                                                                                                gcAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGa                                                                                                                                         <  1:1248265/239‑1 (MQ=255)

                                                                                                        gCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                           <  1:523956/149‑1 (MQ=255)

                                                                                                           actactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGt                                                                                                                              <  1:1209857/239‑1 (MQ=255)

                                                                                                            ctactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑c                                                                                                                            <  1:59509/239‑1 (MQ=255)

                                                                                                             tactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCg                                                                                                                                                         <  1:1313167/210‑1 (MQ=255)

                                                                                                              actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAg                                                                                                                          <  1:1003762/239‑1 (MQ=255)

                                                                                                                 ttGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCg                                                                                                                       <  1:61003/239‑1 (MQ=255)

                                                                                                                  tGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGTCA‑C‑TCTGTCTGCCGGTGAAACGCTTTTTTTCTGCGACCCACGTGAACg                                                                                                                                                                                                                 >  2:523956/1‑149 (MQ=255)

                                                                                                                         gTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTg                                                                                                               >  1:740642/1‑239 (MQ=255)

                                                                                                                             cgcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGcc                                                                                                            <  2:1431805/238‑1 (MQ=255)

                                                                                                                                aGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCcgcg                                                                                                         <  1:1157679/238‑1 (MQ=255)

                                                                                                                                  cTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGGAGGCA‑C‑TCCGTCTGGCGGGGGAACGTTTTTTTTGTGCGACCAACCGGGACGATACCCTGCCCCCGTTTCTTCACGGCGGTCAGTTGGCCCCCCAAGCGACTCAGGCGACGTTATCCCACACGATGGGCGTGGGG‑CAACAGCACAACAGGCCCCgggt                                                                                                      >  2:402648/1‑239 (MQ=255)

                                                                                                                                    gCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGGGGAACGTTTTTTTGCTGCGACCAACGTGAAAGATACCGTGCCACGTTTTCTGCACGACGGGCAGTGGTCACCCAAAGCGAACCCGGCGGCGTTATCCAAACCGACGGGCGTGAGT‑CAGCCGcacaacagcacccgcgtgg                                                                                                    >  2:965964/1‑226 (MQ=255)

                                                                                                                                               cgcgCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTc                                                                                         <  2:1299399/239‑1 (MQ=255)

                                                                                                                                                               tggcgcGGTGCAAAGCGGAAACGTGGGCGGTTGGACGGCGGGTCGGGTGGCGAAGTCA‑C‑TCGGTCGGCCGGGGAAACGTTTTAGTGCTGCGACCAACGTGAACTATACCGGGCCACGTTCCCTGCACGGCGGTCAGTGGTCGCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGGGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGc                                                                        <  2:275029/234‑1 (MQ=255)

                                                                                                                                                                ctaccggcgccaacgggaacggtCGGCGTTTTGACGGCGGGCCTGCGGGCAGAGTCA‑C‑TCGGTCTCCCGGTAAATCGTTTAATTGCTGCGACCAACGTGAACTATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGGGACTCAGGCGACGTTATCCAACGCGATGGACTTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGc                                                                        <  2:463609/216‑1 (MQ=255)

                                                                                                                                                                     ggggCCAAGCGGAACCTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGa                                                                  <  2:927205/236‑1 (MQ=255)

                                                                                                                                                                     ggTGCCAAGCGGAAACTTCGGCGATTTGCCGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGa                                                                  <  1:373783/239‑1 (MQ=255)

                                                                                                                                                                     ggTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGa                                                                  <  1:778524/239‑1 (MQ=255)

                                                                                                                                                                          cAAGCGGAACCTTCGGCGATTTGACGGCGGGTGTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAATGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  2:113142/239‑1 (MQ=255)

                                                                                                                                                                          cAAGCGGAAACTTCGGCGATTTGCCGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  2:237218/239‑1 (MQ=255)

                                                                                                                                                                          cAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAagag                                                             <  1:714427/239‑1 (MQ=255)

                                                                                                                                                                            aGCGGAACCTTCGGCGTTTGGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCt                                                           <  2:143896/239‑1 (MQ=255)

                                                                                                                                                                                gAACCTTCGGAGAGTTGGCGGCGGGTCTGCTGGCGGAGTCA‑C‑TGGGTCGGCCGGTGAAACCTTTTATTGCGGCAGCCAACGTGAACGATACCGTGCAGGGTTGCCTGCACGGCGTGCAGTGGTCACCCAAAGCGACTCAGTCGACGTTATCCAACGCGTTGGACGTGAGT‑CAGCCGAACAACTGGCCGCTTGTGGAAGCGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTggg                                                        <  2:355700/238‑1 (MQ=255)

                                                                                                                                                                                gAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTggg                                                        <  1:578111/238‑1 (MQ=255)

                                                                                                                                                                                    cTTCGGCGATTTGACGGGGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                   <  2:854504/239‑1 (MQ=255)

                                                                                                                                                                                    cTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                   <  1:496802/239‑1 (MQ=255)

                                                                                                                                                                                        ggCGATTTGACGGCGGGTCTGCTGGCGAAAGCA‑C‑TCGGGCTGCCGGCGAAACGGTTTATTTCTGCGGGCAACGTGAACGATACCGTGGCACGGTTCCTGCACGACGGGCAGGGGGGCACCAAAGCGACTCAAGGGGCGGTATCCAACACGCTCGGCGAGAGTACAACGGAACAACT‑GCCGCGGGTGGGAGAGTGGTTCCCCCGCCAACGTTGGGCAAC‑GAAACAGCTGGGGTATGCAg                                                >  2:1214049/1‑238 (MQ=255)

                                                                                                                                                                                           gATTTGACGGGGGGTCTGCTGGCGAAGTTA‑C‑TCGGTCTGCCGGTCAAACGTTTTATTGCCGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGCCTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                             <  2:259970/238‑1 (MQ=255)

                                                                                                                                                                                           gATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAATCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                             <  1:1288822/238‑1 (MQ=255)

                                                                                                                                                                                             ttggagggcggtgttgctgGCGAAGGCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATGCCGGGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTg                                           <  2:288478/224‑1 (MQ=255)

                                                                                                                                                                                                     cggGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATGGCTGCGACCAACGTGAACGATACCGGGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGAtgatga                                   <  1:811895/238‑1 (MQ=255)

                                                                                                                                                                                                         gccgctgGCGAAGTCC‑C‑CCGGTCTGCCGGTGAAACGTTTTATTGCTGCGCCCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACGCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                               <  2:821767/235‑1 (MQ=255)

                                                                                                                                                                                                          ctgctgGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCAc                             <  1:1522407/239‑1 (MQ=255)

                                                                                                                                                                                                           tgctgGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACTTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGa                                                                  <  1:667039/201‑1 (MQ=255)

                                                                                                                                                                                                           tgctgGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGa                                                                  >  2:667039/1‑201 (MQ=255)

                                                                                                                                                                                                           tgctgGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGGGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCAc                             >  2:1518295/1‑238 (MQ=255)

                                                                                                                                                                                                           tgctgGCGAAATCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGGGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTTGGTTATGCAGCCGTGGATGAAGAAACCAc                             >  2:984771/1‑238 (MQ=255)

                                                                                                                                                                                                              tgGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                         <  2:740642/239‑1 (MQ=255)

                                                                                                                                                                                                              tgGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                         <  1:803635/239‑1 (MQ=255)

                                                                                                                                                                                                                  gAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAg                      >  1:174422/1‑238 (MQ=255)

                                                                                                                                                                                                                   aaGTCA‑C‑TCGGTCTGCCGGTGAACCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGa                     <  1:212705/238‑1 (MQ=255)

                                                                                                                                                                                                                    aGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACa                   <  2:548364/239‑1 (MQ=255)

                                                                                                                                                                                                                          c‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg              <  1:999227/239‑1 (MQ=255)

                                                                                                                                                                                                                          c‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg              <  1:1063755/239‑1 (MQ=255)

                                                                                                                                                                                                                           cacgggcgcccGGTGAAACGTTGTATGTCTGCGCCCAACGTGACCGATACCGTGCCACGTTTCCAGCACGACGGTCAGTGGTCCCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg              <  2:748200/230‑1 (MQ=255)

                                                                                                                                                                                                                            tCGGTCTGCCGGTGAAACTTTTTATTGCGGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCCCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt             <  2:1265778/239‑1 (MQ=255)

                                                                                                                                                                                                                            tCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt             >  2:1500253/1‑239 (MQ=255)

                                                                                                                                                                                                                                tCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               >  1:203107/1‑91 (MQ=255)

                                                                                                                                                                                                                                tCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               <  2:203107/91‑1 (MQ=255)

                                                                                                                                                                                                                                        tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               >  2:671617/1‑83 (MQ=255)

                                                                                                                                                                                                                                        tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                               <  1:671617/83‑1 (MQ=255)

                                                                                                                                                                                                                                        tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa  >  1:743729/1‑238 (MQ=255)

TCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑C‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCG‑CAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGA  >  NC_000913/4300‑4767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`