Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,256,149 T→G 11.5% H106P (CAC→CCC)  plsB ← glycerol‑3‑phosphate O‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,1490TG11.5% 304.9 / 2.1 124H106P (CAC→CCC) plsBglycerol‑3‑phosphate O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (39/66);  new base G (6/12);  total (46/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATC  >  NC_000913/4255914‑4256384
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
tgaacccgtgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTgg                                                                                                                                                                                                                                           <  2:69454/233‑1 (MQ=255)
 ttgacgggaccggttcaagcccccATACGGCGCAGCGAAACTGACGGGGAGAAACGCCCAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:530803/217‑1 (MQ=255)
 ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  1:1067615/239‑1 (MQ=255)
 ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  1:1189943/239‑1 (MQ=255)
 ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:228098/239‑1 (MQ=255)
 ttATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  1:498482/239‑1 (MQ=255)
 ttACCCGTGCCGTGTTCATCCGCCACCCGGCCCCGCGAACCTGACGGCGAGAAACGCACAAACCTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:157295/239‑1 (MQ=255)
 ttACCCGTGCCGGGTCCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:340067/239‑1 (MQ=255)
 ttACCCGGGCCGGGTCCATCCGGCATACGGCGCAGCGAAACTGACGGCGAAAAACGCACAAAACTGTCGCGACCGGG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:1161246/239‑1 (MQ=255)
 ttACCCGGGCCGGGGCCACCCGCCATACGGCGCAGCGAAAATGACGGCGAAAAACGCACAAAACTGCCGCGACCGGG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:413979/239‑1 (MQ=255)
 ttAACCGTGCCGGCTCCAGCCGCCATGCGGCGCAGCGAAACGGACGGCGAGAAACCCAAAAACCTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAT‑TTTC‑GTTACGCCGTTAAGCATACGCGGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:970535/239‑1 (MQ=255)
 tgaTCCGGGACGTGTGCACCCACCATACGGCACAGCGAAACTGACGGCGAGAAACGGAAAAAGCGGTCTCGACCGAG‑CCACA‑GTCCAGCGAAAAA‑TTTC‑TGTACGCCAGTAAGCAGACGCAGCGGCGGGTTCAGTTGGCCTTTTT‑CCCGCCCCGGCGCGCGGCC‑AACCATC‑CCCGACGCTGGCACCAGCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:1088057/237‑1 (MQ=255)
 taaccggtgccgggTTAACCCGCCATACGGCGCAGCGAAACGTACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCGCA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:789170/233‑1 (MQ=255)
  gagcagcgacgtgGTCGTCCGCCATGCGGCGCGACGAAACTGGCGGCGAGAAACGCGCAACACTGGGGGGACCGGG‑CCGAA‑GTACAGCGAAAAA‑TTTC‑TGGACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCATTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCGGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGaa                                                                                                                                                                                                                                         <  2:34262/229‑1 (MQ=255)
        tgccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TTCCCCCCCGCGCGCCACC‑AAACATC‑ACCCACACTCGCACCATCTCCACACCCAGATTTTGGTTGCTTCGGTGCCAATCAAG‑ATAGACGTGGGACAGGttt                                                                                                                                                                                                                                  >  2:572923/1‑238 (MQ=255)
         gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCGGCGCGCCACC‑CAACAAC‑ACCCACCCCGGCCCCATCTGCACCTCCAGATTTGGGTTGCTACGGTGCAAAACAGG‑GAAGTAGGGGGACAGCTTaa                                                                                                                                                                                                                                 >  1:34596/1‑239 (MQ=255)
         gccgtgTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCCCTTCGCCTTTTT‑TACCCCCCGGCGCGCGGCC‑CAACACC‑CCCCACCCCGGCACCCTCTCCCCCTCCCGATTTTGGGTGCTACGGGGGAAATCGAG‑AAAGGCGTGGGACAGCATaa                                                                                                                                                                                                                                 >  2:868270/1‑239 (MQ=255)
              gtTCATCCGCCATACGGCGCAGCAAAACTGACGGCGAGAAACGCACAAAACGGCCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGGGGCGGGTTCACTTCGCCTTTTG‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                                                                                                            <  1:1093365/239‑1 (MQ=255)
              gtTCACCCGCCATACGGCGCAGCGAAACGGACGGCGAAAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                                                                                                            <  1:700338/239‑1 (MQ=255)
               ttCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGAc                                                                                                                                                                                                                            <  1:885972/238‑1 (MQ=255)
               tgctaccGCCAAGCGGGGCAGCGAAACTGTGGGGGAGAAACCAACAAAACGGTGGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATGCGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑GCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGAct                                                                                                                                                                                                                           <  2:1406699/234‑1 (MQ=255)
                    ccGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑TACGCCCCCGCGCGCGACC‑CAACATC‑ACCGGCACCGGCACCCTCTTCCCATCCCCATTTTGGGTGCTACGGGGCCAATCGAG‑ATAGTCGGGGAACAGCTTAAAAAACtcctct                                                                                                                                                                                                                      >  1:278446/1‑239 (MQ=255)
                      gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                                                                                                    <  1:991722/239‑1 (MQ=255)
                       ccATACTGCGCAGCGAAACTGACAGCGAGAAACGCACAAAACAGTCGCTACCGAG‑CCACA‑GTACAGCGAAAAC‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGATTCCTTTGCCGCTTT‑TTCGGCCCCGCTCGCGGCC‑CTACATCAACCGACCCTTCCA‑CACCTCTACACCCCGACTTGGATTGCTTCGGTGCCAATCGAG‑ATAACCGCTGACACGTTTAATAGACCCTTCttt                                                                                                                                                                                                                    >  2:5728/1‑238 (MQ=255)
                         aTACGGCGCAGCGAAGCTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                                                                                                 <  1:412119/239‑1 (MQ=255)
                         aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                                                                                                 <  1:500569/239‑1 (MQ=255)
                         aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                                                                                                 <  1:244740/239‑1 (MQ=255)
                         aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                                                                                                 <  1:1479587/239‑1 (MQ=255)
                         aTACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTT‑TTCCCCCCGGCGCGCGGCC‑AAACATC‑ACCCACCCTGGCACCCACCTCCCACCCAGATTTTGGTTGCTTCCGGGCAAATCGAG‑ATAGTGGTGGGACAGCTTAATAAACACTTCTTTTgg                                                                                                                                                                                                                 >  2:1479587/1‑239 (MQ=255)
                         aTACGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCgg                                                                                                                                                                                                                 <  1:1195483/239‑1 (MQ=255)
                           aCGGCGCAGCGAAACTGACGGCGAGAAACGCAAAAAACGGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCg                                                                                                                                                                                                               <  1:482940/239‑1 (MQ=255)
                               cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCgtgt                                                                                                                                                                                                            <  1:1358143/238‑1 (MQ=255)
                               cgcAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACCTCGCCTTTTT‑CACCCCCCCGCCCGCGACC‑AAAAAAC‑AACCGCACCGGCCCCCCCCTCACAACCACACTTGGGGTGCTTCGGTGCAAACCCAG‑ATAATCGTGGGACCGCTTAATAGCATCTTTTTTCTGCGTGTa                                                                                                                                                                                                           >  2:1382704/1‑239 (MQ=255)
                                gcACCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGCCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTaa                                                                                                                                                                                                          <  1:878974/239‑1 (MQ=255)
                                  aGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCCGATTTTGGTTGCTACGGGGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAAACTCTTCTTTTGGCGTGTAATa                                                                                                                                                                                                        >  1:1432040/1‑239 (MQ=255)
                                     gAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                                                                                                                                                                                                      <  1:1286181/238‑1 (MQ=255)
                                     gAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAgg                                                                                                                                                                                                      <  1:773432/238‑1 (MQ=255)
                                                       gcaaaaaacCTGTCGCGCCCG‑G‑CCCCAGGTACA‑CCAAAAA‑TTTCTTGTCCGCCGTAAGGCATCCGCGGGGGGGGTTTCACTCCGCTTTTTT‑CCCCCCCCGGCGCGCGACA‑AAACCCC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGAAACAGCTTAATAGACTCTTCTTGCGGCGTGTAATAGGTGAACACACGCGGCccgcc                                                                                                                                                                                   <  2:1120687/234‑1 (MQ=255)
                                                       cccaaaaaaCCGTCGCGCCCGGGCCCGCA‑GTCCAGCAAAAAT‑TTTG‑TGTCCCCGGTAAAGCACCCGCCGCGGCGGTTCCACTTCCCCTTTTC‑CCCCCCCGGGCGCGCACCA‑AAACACC‑CCCGACACGGGCACCATCTGAACATCCAGATTGGGGTTGCTACGGTGAAAATCGAG‑ATAGGCGGGAAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATGGGTGAACACACGCGGCccgc                                                                                                                                                                                    <  2:817642/234‑1 (MQ=255)
                                                          aaaaacCTGCCCCGACCGAG‑CCACA‑GAACAGAAAAAAT‑TTTT‑TTGCCCCCGTAAAGCATCCGCGGCGGGGGTTTCCCTCCGCTTTTTC‑CCCCCCCCGGCGCCCGACA‑AAACATC‑CCCGACACGGGCACCATCTGCACACCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGTTTAATAGACTCTTCTTTCGGCGTGAAATAGGTGAACACCCGCGGCCCGCCGtg                                                                                                                                                                                <  2:556543/237‑1 (MQ=255)
                                                                ccgccgccaccggccccACA‑GTACAGCAAAAAT‑TTTC‑TGTCCACCGTAAACATTCCGCGGGGGCGGTTTCCCTCCCCCTTTTC‑CCCCCCCCGCGGCGCGACA‑AAACACC‑CCCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGtgaatga                                                                                                                                                                           <  2:565428/224‑1 (MQ=255)
                                                                     gcggacagggctaca‑gcacagcAAAAAT‑TTGC‑GTTCCGCGGTGAAGCAAAGGACGGGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACA‑AAACACC‑CCCGACACTGGCACCATCTGCACAGCCAGATTGGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGGGGAACAGCTTAATAGACTCTTTTTTCGGCGGGTAATAGGTGAACACACGGGGCCCGCCGTGAATGAAcaca                                                                                                                                                                      <  2:1472023/227‑1 (MQ=255)
                                                                       gACCGAG‑CCAAA‑GTACAGCAAAAAT‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCTTTTTT‑CCCGCCCCGGCGCGCGACC‑AAACACC‑CCCGACACTGGCACCATCTGCACATCAAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAg                                                                                                                                                                   <  1:1248105/239‑1 (MQ=255)
                                                                        gccGAG‑CCACA‑GTACAGAAAAAAT‑TTTT‑TGTCCCCGGTTAAGCATCGGCGGGGGGGGTTTCCCTTCCCTTTTTC‑CCCGCCCCGGCGCGCGAAA‑AAACATC‑CCCGACCCGGGCACCATCTGCACATCAAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAg                                                                                                                                                                   <  2:1331567/237‑1 (MQ=255)
                                                                        aCCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTTGCCTTTTT‑CACGCCCCCGCCCGCGACC‑AAAAATC‑ACCGACACCGGCACAATATGCACAACCAGCATTGGGTTGCTCCGGGGCAAAACGAG‑ATAGTGGTGGGACAGCTTAATAGAGCCCTCTTTCGGCGGGTAAAATGTGAACAAACCCGGCCCCGCGCGGATGAAACCATAg                                                                                                                                                                   >  2:175532/1‑238 (MQ=255)
                                                                          cGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTAACTTTCCCTTTTT‑CACGCCCCCGCGCGCGACC‑AAAAATC‑AACGCGCCCGGCACCATCTGCACCTCCAGATTTAGGTTGCTACTGTGCAAAAACAG‑ATAGTGGGTGGACAACTTAATCAACTCTTTGTTAGGCGAGTAATAGGAGAAGCAACACGGGCTGCCGTGAAGAAACAAATAacgg                                                                                                                                                                >  2:520836/1‑238 (MQ=255)
                                                                            cgagcccacgTACAGAGAAAAATTTTT‑TGGCCGCCGTAAAGAATCGGCGGGGGGGGGTTCCTTCCGCTTTTTT‑CACCCCCCGCGGCG‑GACCAAAACACC‑ACCGACCAGGGCACAATCTGCACATCCAGATTGGGGTTGCTACGTGGCAAATGGAG‑ATAGTGGTGAAACAGTTTAATAGACTCTTCTTTCGGCGTGAAATAGGGGAACACACGCGGCCCGCCGTGAATGAACACATAgcg                                                                                                                                                                 <  2:1148883/230‑1 (MQ=255)
                                                                             g‑CCACA‑GTCCAGCAAAAAT‑TTTC‑TCTACGCCGTAAACCACACGCCGCGGCGGGTCCACTTCCCCTTTTC‑CACGCCCCGGCGCGCAACC‑AAACACC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGTTTGCTCCGGTGCAAATCGAG‑ATAGTCGTGAAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGACCCCCCGCGGCCCGCCGTGAATGAACACATAGCGCgg                                                                                                                                                              <  2:34596/238‑1 (MQ=255)
                                                                                         aGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCCCGCGCGCGCCC‑CAACATC‑ACCGCCACCGGCACCCTCTGCACATCCCGATTTGGGTTGCTACCGTGCAAATCGCG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGAGAACACACGCGGCCCCCCGTGGATGATACCATAGCGCTGCAGTAGCGTg                                                                                                                                                    >  2:731713/1‑238 (MQ=255)
                                                                                         aGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGGTGCTAATGTGCAAAACGAT‑ATATTCTTCTATTCGCGTTATAGACTCTTCTTTCGGCGGGTAATAGGGGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGGAGCGTg                                                                                                                                                    >  1:900844/1‑238 (MQ=255)
                                                                                           cGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTCCCTTTTT‑TACGCCCCCGCGCGCGGCC‑CAACATC‑ACCCACACCGGCACCCTCTTCACCTCCCGATTTTGGGTGCTACGGTGCAAAACGAG‑GAAGTCGTGGGACAGCTTAATAAACTCTTCTTTTGGCGGGTAATAAGGGAACACACCCGGCCCCCCGGGGATGCACACCTAACGCGGCAGAAGGGCGcc                                                                                                                                                  >  2:24404/1‑238 (MQ=255)
                                                                                           cGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑TACGCCCCCGCGCGCGACC‑AAACATC‑ACCCACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAAACGAG‑ATAGTCGTGGAAAAGCTTAATAGACTCTTCTTTCTGCGGGTAATAGGGGAAAACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCg                                                                                                                                                 >  1:954243/1‑239 (MQ=255)
                                                                                                        tGTCCGCCGTTAAGCATACGCAGCGGCGGGTTCATTTCCCCTTTTT‑CCCGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACGGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAg                                                                                                                                      <  1:501511/239‑1 (MQ=255)
                                                                                                           cccccGTAAACCATACGCGGCGCGGGGTTCCTTTCCCTTTTTC‑CCCGCCCGGGCGGGCGACA‑AACCACC‑ACCGACACGGGCCCCATCTGCACATCCAGATTTGGGTTGCTCCGGTGCAAATCGAG‑AAAGTCGTGAAACAGCTTAATAGCCTCTTCTTTCGGCGTGAAATAGGTGACCCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCgg                                                                                                                                   <  2:900844/236‑1 (MQ=255)
                                                                                                                    aGCATACGCAGCGGCGGGTTCACTTCCCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCCACCCCGGCCCCATCTTCACATACCGATTTTGGTTGCTACCGGGCAAATCGCG‑ATAGTCGGGGGACAACTTTATAGACCCTTCTTTTGGCGTGTAATAAGTGAACAAACGCGGCCCCCCGGGTATGAACACACAGCGCGGCAGGAGCGTGCCGGCgcgtttcacgggggctcacgg                                                                                                                          >  2:565704/1‑219 (MQ=255)
                                                                                                                      cATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCttt                                                                                                                                                                                                                    <  1:1042940/147‑1 (MQ=255)
                                                                                                                       atccGCAGCGGCGGGTTCCCTTCCCCTTTTC‑CACCCCCCGGCGCGCAACA‑AAACATC‑CCCGACACTGGCCCCATCTGCACATCCAAATTGGGGTTGCTACGGTGAAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                                                                                                        <  1:35781/235‑1 (MQ=255)
                                                                                                                       aacccccgcggcgGGTTCCCCTGCCCTTTTTCCCCCCCCCGGCGCGCGCCA‑AAACACC‑CCCGACACTGGCCCCATCTGCACATCAAGATTGGGGTGGCTCGGGTGAAAATCGAG‑ATAGTCGTGAAACAGCTAAATAGACTCTTCTTTCGGGGTTTAATAGGTGACCACCCGCGGCCCGCCGTGAATGAACATATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGt                                                                                                                        <  2:1217878/232‑1 (MQ=255)
                                                                                                                                 gtaaaagaaccagctcTTTTC‑CCCCCCCCGGCGCGCGACA‑AAACACC‑ACCGACACTGGCACCATCGGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGGGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATGGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                                                                                              <  2:834698/223‑1 (MQ=255)
                                                                                                                                  ggtgctccgtctcccctttgacgcGCCCCCGCGCGCCGCC‑AACCCTC‑CCCGACACGCGCCCGTTCTGCACATCGAGATTTGGGTTCCTGCGGGGCAACGCGAG‑ATAGTCGGGGAAAAGCTTAATAGACTCTTCTTCCGGCGGTAAATAGGTCAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTc                                                                                                              <  2:640906/215‑1 (MQ=255)
                                                                                                                                                          gCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGa                                                                                                    >  1:834698/1‑224 (MQ=255)
                                                                                                                                                               ggCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCa                                                                                 >  1:273146/1‑238 (MQ=255)
                                                                                                                                                                    gcgACA‑AAACACC‑CCCGACACTGCCACCATCTGCACATCCAGATTTGGGTTGCTAGGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAAt                                                                           <  1:616669/239‑1 (MQ=255)
                                                                                                                                                                           aaaCACC‑ACCGCCACGGGCACCACTTGCACACCAAGATTGGGGTTGCTAGGGTGGAAAGCGAG‑AATGGCGGGAAAAAGTTAAATAACCTCTTCTTTCGGGGTGAAATAGGTAAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCtt                                                                     <  2:273146/239‑1 (MQ=255)
                                                                                                                                                                            agagccagcagACCCTGGCACCATCTGCACAACCAGATTTGGGTTGCTAGGGTACAAATGGAG‑ATAGTCGTGGAACAGCTAAATAGACTCTTCTTGCGGCGTGTAATAGGTAAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCtt                                                                     <  2:1186123/229‑1 (MQ=255)
                                                                                                                                                                            acacgccccggACACTGGCCCCATCGGCACATCCAGTTTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGGGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCtt                                                                     <  1:1400558/229‑1 (MQ=255)
                                                                                                                                                                                 tccccGACACGGGCACCATCTGCACATCCAGATTGGGGTTGCGACGGTGAAAAGCGAG‑ATAGTCGGGGAACAGCTTAATAGCCTTTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAg                                                                <  1:1493841/236‑1 (MQ=255)
                                                                                                                                                                                 ccccccccgcgggctccttcgccacaTCCAAATTTGGGTTCTCACGTGACAAAAGGAG‑TTGGGCGGGAAACAGCTTAATAGCCTCTTCTTCGGGGGTGTAAGAGGGGACCACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAg                                                                <  2:688769/217‑1 (MQ=255)
                                                                                                                                                                                   ccgcgcatggcccccATCTGGCCATCTAGATTTGGGTTCATACGGTCAAAATCGAA‑ATGGGCGTGGACCAGCTTAATAGCCTTTTCTTGCGGCGTGTAAGGGGTGAACCCCGGGGGGCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGtt                                                              <  2:121036/226‑1 (MQ=255)
                                                                                                                                                                                          cTGGCCCCATTGTCACACCAAGAGTTGGGTTGCTACGGTGAAAATCGAG‑ATAGTCGGGAAACATCTTAAAAGCCTCTTCTTGCGGCGTGTAATAGGTAAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGt                                                       <  2:1432040/239‑1 (MQ=255)
                                                                                                                                                                                            ggCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaa                                                     >  2:1474341/1‑239 (MQ=255)
                                                                                                                                                                                              cACCATCTGCACATCCAGATTTGGGTTGCTAGGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                    <  1:987379/238‑1 (MQ=255)
                                                                                                                                                                                              cACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGTTTAATAGACTCTTCTTTCGGGGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTaaa                                                    <  1:592152/238‑1 (MQ=255)
                                                                                                                                                                                               aCCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTa                                                           >  1:1186123/1‑230 (MQ=255)
                                                                                                                                                                                                  aTCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGt                                                >  1:895474/1‑238 (MQ=255)
                                                                                                                                                                                                  aTCTGCACACCCAGTTTTGGTTTGCTACGGTGCAAATCGGG‑ATAGTCGTGAAACAGCTTAATAGTCTCTTCTTTCGGCGTGTAATAGGTTAACCCCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTa                                               <  1:341639/239‑1 (MQ=255)
                                                                                                                                                                                                   tCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTAc                                              >  2:364984/1‑239 (MQ=255)
                                                                                                                                                                                                   tCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTAc                                              >  2:258510/1‑239 (MQ=255)
                                                                                                                                                                                                       cacaTCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATaa                                          >  1:290574/1‑239 (MQ=255)
                                                                                                                                                                                                        acaTCCAGTTTGGGGTTGCTAGGGAGCAAAT‑GAGAAAAGTCGTGGAACAGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGAGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAAt                                         <  2:954243/239‑1 (MQ=255)
                                                                                                                                                                                                        acaTCAAGATTGGGGTTGCTACGGTGAAAACCGAG‑ATAGTCGTGGAACGGCTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAAt                                         <  1:129079/239‑1 (MQ=255)
                                                                                                                                                                                                         caTCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAAtt                                        >  1:737939/1‑239 (MQ=255)
                                                                                                                                                                                                            ccAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTgg                                      >  2:1180617/1‑238 (MQ=255)
                                                                                                                                                                                                            ccAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTgg                                      >  1:1516931/1‑238 (MQ=255)
                                                                                                                                                                                                             gaGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTAAATAGACGCTTCTTCCGGCGGGTAATAGAGCAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                    <  1:1507122/238‑1 (MQ=255)
                                                                                                                                                                                                             cAGATTTGGTTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTCCGGCGTGTAATAGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGCCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                    <  1:572923/239‑1 (MQ=255)
                                                                                                                                                                                                             cAGATTTGGGTTGCTACGGTGAAAATCGAG‑ATAGTCGTGGAACAGTTTAATAGCCTCTTCTTTCGGCGTGTAATAGGTGAACCCACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGAc                                    <  1:24404/239‑1 (MQ=255)
                                                                                                                                                                                                               gATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACga                                  >  2:1520938/1‑239 (MQ=255)
                                                                                                                                                                                                                    gggTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGt                             >  1:1015432/1‑239 (MQ=255)
                                                                                                                                                                                                                       ttGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTAt                           >  2:770314/1‑238 (MQ=255)
                                                                                                                                                                                                                          cTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCa                        >  1:998107/1‑238 (MQ=255)
                                                                                                                                                                                                                          cTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCa                        >  1:1174806/1‑238 (MQ=255)
                                                                                                                                                                                                                           tagggggCAAACGGAG‑AGAGGCGGGAAACAGCAAAAGAGACGTTTCTGGCGGGGTGAAAGGGGGGAACACGCGCGCCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAg                       <  2:135470/232‑1 (MQ=255)
                                                                                                                                                                                                                               gtacAAATCGTG‑ATGGTGGGGAAACACCTTAATCGCCTCTTCTTCGGGGGTGTAATGGGTAAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGcccc                   <  2:1260624/235‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCCGCTCTAACGCGTCAGGCAAGTCATGTGCCAGACACTTGCCGCGCAACGTCCGCAAATCTGCTTTCGAGTTGTACGGTCAAACGTACATAATTGGCCAAACGGCATCCAGCCCCatt                >  2:192178/1‑236 (MQ=255)
                                                                                                                                                                                                                                   aaaTCGAG‑AGGGTGGTGAAACAGCTAAATAGACTCTTCTTGCGGTGTGAAATAGGTGAACCCGCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTc              <  2:537349/239‑1 (MQ=255)
                                                                                                                                                                                                                                   aaaTCGAA‑ATAGTCGTGAAACAGCTTAATAGACATTTCTTCCGGCGTGTAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTc              <  1:1155857/239‑1 (MQ=255)
                                                                                                                                                                                                                                         ag‑aTAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGc        >  1:1304391/1‑239 (MQ=255)
                                                                                                                                                                                                                                             tAGTCGTGGAACAGCTAAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAgg     <  1:149660/239‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGTGGATCAGCTTAATAGACTCTTCTTGCGGCGTGAAATAGGGAACCACACGGGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCAATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATc  <  1:5728/238‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           
TTTATCCGTGCCGTGTTCATCCGCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAG‑CCACA‑GTACAGCGAAAAA‑TTTC‑TGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCCGGCGCGCGACC‑AAACATC‑ACCGACACTGGCACCATCTGCACATCCAGATTTGGGTTGCTACGGTGCAAATCGAG‑ATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAACGTCAGCAAATCTGCTTTCGAGTTGTACGGTAAAACGTACATAATTGGACGAGAGGTATCCAGCCCCAGTTCCGGGGCAGGATC  >  NC_000913/4255914‑4256384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑