Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,536,219 C→A 23.3% intergenic (+187/+396) yjhR → / ← nanS pseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,536,2190CA23.3% 126.2 / 3.7 54intergenic (+187/+396)yjhR/nanSpseudogene, helicase family, putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase
Reads supporting (aligned to +/- strand):  ref base C (15/24);  new base A (8/5);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAAATGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATAACA  >  NC_000913/4535981‑4536450
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
aaaaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACATCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagc                                                                                                                                                                                                                                              <  2:598411/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  1:367037/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  2:229236/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  2:290610/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  1:1478062/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  1:1126599/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  1:1060263/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGACTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  1:110434/239‑1 (MQ=255)
  aaaaTGAAATAAAGGCAATATATGGTAGTATGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  2:400633/239‑1 (MQ=255)
            aaaGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAAtaatataata                                                                                                                                                                                                                                <  2:1271/239‑1 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGCTTATAAATCTTATTGTTTACCTATTCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGTTTTGAAGAAAAAGCAAACAACAAAATAAAAAATGAATAATttttttta                                                                                                                                                                                                              >  2:593972/1‑237 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGTGGCTTTTTTGTAAGTAAAAAAAATACAGCACTAATATATAAATAGGCTAATTTTTTTgt                                                                                                                                                                                                              >  1:284048/1‑238 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTTCTTGTTTGAATGAAAGAAAATATAAACAATAATATAATAAAGGCCGAATTTTTTTgt                                                                                                                                                                                                              >  1:1175373/1‑238 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGTTTTTTTTAATTAAATAAAAAGTAAGCGAaaaatatcttcttatatactattttac                                                                                                                                                                                                                 >  2:199442/1‑218 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGTTTTTTGTTATTAAAGTAAAATACAGAAAAAATATAATAATAGGCTAATtttttttt                                                                                                                                                                                                              >  2:610994/1‑237 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTATGTATGTAATAAAAAACAAACAATAATAtaataaataaccaattttttttt                                                                                                                                                                                                              >  2:247883/1‑222 (MQ=255)
                              tCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGCTTTTTGGAATGAAAAAAAAAACAAGGAAATATAtaaaaaagtacatattttttttt                                                                                                                                                                                                              >  1:1308699/1‑222 (MQ=255)
                               cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGaa                                                                                                                                                                                                             <  1:1195851/239‑1 (MQ=255)
                               cTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATAAAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGGCTAATTTCTTTGaa                                                                                                                                                                                                             <  2:178362/239‑1 (MQ=255)
                                tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCCTTTTTGGAATAAAAAAAAAGAAAACAAACATATTATAAATGCCTTATtttttttttc                                                                                                                                                                                                            >  1:1067348/1‑235 (MQ=255)
                                tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTGTATTATAAAAAACATAAAAAATAATATaattatatatttaattttttttaa                                                                                                                                                                                                            >  2:1195851/1‑221 (MQ=255)
                                tGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTGAATGAAAGAAAAAACAAGAAAAAAAATAATAAAGGCCTAATTTTTTTTaaa                                                                                                                                                                                                            >  2:516179/1‑238 (MQ=255)
                                        cGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTTTATGTAAAAAAAAACAATCAATAATGAAAAAAAGGACTTATTTCtttttacacctctct                                                                                                                                                                                                    >  2:691560/1‑227 (MQ=255)
                                                           gaGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTAt                                                                                                                                                                               <  2:400103/239‑1 (MQ=255)
                                                                 ttCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGTATGAAAAAAAAAGCAAGCAAAAATATAATAATAGGCTCATTTTTTTTAACATTATTAAGTATTATAATATTGTCTAtttttct                                                                                                                                                                         >  1:787183/1‑235 (MQ=255)
                                                                      gATACAGGAGTAGTAAAGGGCATATTGTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCata                                                                                                                                                                                              <  1:464543/214‑1 (MQ=255)
                                                                         aCAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGt                                                                                                                                                                 <  1:691560/239‑1 (MQ=255)
                                                                           aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTGGAATGAAAGAAAATGGAAGCAATAATATAATAATTGCCTAATTTTTTTTAACATCATTTAGCATATCATCATTGTCTATTTATCAGAATCCATCg                                                                                                                                                               >  1:396881/1‑239 (MQ=255)
                                                                           aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGAGCTTTTTGGAATGAAAAAAAATCACAGAAATAATATAATAAAAAGCTAATTTCTTTTTAAATCATTAAGGAAATCATCAATGTCTCTTTTCCATAATCCGTcc                                                                                                                                                               >  1:191377/1‑238 (MQ=255)
                                                                             gAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTGCATGAAATATAAAACCATAAAAAATATAAAAATAGGATTATTTCTTTTTACATAATTTATAATAATATCATCATCCTTTTTTCCATATCCGTCGaa                                                                                                                                                             >  2:887807/1‑238 (MQ=255)
                                                                                tagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGTCTTTTTTAATGATAAAAAAAACAAAGCAAAATATAAAAATAGTCTCATTTCTTTTTACATCTATTAGAAAATAATCATCCTCCTTTTGcttatacccatcctactc                                                                                                                                                          >  2:464543/1‑222 (MQ=255)
                                                                                tagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTTGAATGAAAGAAAAAACAAGAAATAAAATAAAAAAAGTCTAATTTTTTTTTAAATCATTAAACAAATCATCATCGTTTATTTTTCAGAATACGTCTTCTta                                                                                                                                                          >  2:822284/1‑239 (MQ=255)
                                                                                tagtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGGTTTTTTTGATGTAAAAAAAAGAAAACAATAAAATAAAAAAGGCCTAATTTTTTTTTAAATAATTAAGAAAATAATCAATGTTTATTTTTCAGAATCCCGCGACttt                                                                                                                                                          >  2:1390337/1‑238 (MQ=255)
                                                                                  gtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGGGCTTTTTTGAATTAAAAAAAAAAAAAGAAATAATATAAAAAAAAGCTAATTTTTTTTTAAAACATTAAACATATAATCATCGTCTTTTTTTCCGAATACCTTGACttttt                                                                                                                                                        >  2:1210959/1‑236 (MQ=255)
                                                                                    aaaGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTtata                                                                                                                                                       <  1:1315525/237‑1 (MQ=255)
                                                                                               tttACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACgg                                                                                                                                          <  1:593972/239‑1 (MQ=255)
                                                                                                    cAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCatat                                                                                                                                     <  1:375504/239‑1 (MQ=255)
                                                                                                      aTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCa                                                                                                                                   <  1:317789/239‑1 (MQ=255)
                                                                                                       tGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTGGATGAAAGAAAATGAAAGCAATAAAATAATAAAGGCCTAATTTTTTTTTACATCATTAAGAATATCATAATCGTCTATTTTTCAGAATCCGTGCACTTTTAATTCCCCGAAAGGCatatacg                                                                                                                                  >  2:30893/1‑236 (MQ=255)
                                                                                                        gTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTa                                                                                                                                                                                                     <  1:529720/174‑1 (MQ=255)
                                                                                                          tAAGGCGACTTTCCTTTTTATTTCTAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGa                                                                                                                                <  2:450935/238‑1 (MQ=255)
                                                                                                                          tttATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCtg                                                                                                               <  1:1532319/239‑1 (MQ=255)
                                                                                                                                      cGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACg                                                                                                   <  1:785873/239‑1 (MQ=255)
                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:1447703/239‑1 (MQ=255)
                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:1390337/239‑1 (MQ=255)
                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:1210959/239‑1 (MQ=255)
                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:247883/239‑1 (MQ=255)
                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:30893/239‑1 (MQ=255)
                                                                                                                                                gATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTaaa                                                                                         <  1:822284/239‑1 (MQ=255)
                                                                                                                                                         tCTTATTGTTTACCTAATCATTTTCCTATGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATAtaataa                                                                                                                                                                                                                               <  1:642527/99‑1 (MQ=255)
                                                                                                                                                             aTTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTGAATTAAAAAAAAAGTAAGAAAAAAAATAAAAAATGGCCTATTTTTTTTTAAATAATTAAATATATAATTATTTTATTTTTTTCCGAATCCGCCGACTCATAAATACCCACAAAGCATATCTGGACGGGGCTCCCAACCCGCTGGGCCAAAAAGTTCTCCTAAACCGTGTGGATGtg                                                                            >  2:42365/1‑238 (MQ=255)
                                                                                                                                                                           cATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                                                                                                                                                                           <  1:1323397/70‑1 (MQ=255)
                                                                                                                                                                                    ttGCATAATGCGCCTGGGATTAATGGCGGGTGGTTTTTTTGATGAAAAAAAAAGAAAGGCATAATATAAAAATGGGCTTATTTTTTTTTACATAATTAAGCATATTATCATCGTCTATTTGCCTGAAAATCTCCACTTATAATAACCAAAAACGCATATTAAGAAGGTGCGGGCGACGCTGTTGCGCCAAACAGTCCAGCAAAAAGAGTGGAAGGTCGTCGGTTTCCGAGCCCTAATca                                                     >  2:697682/1‑238 (MQ=255)
                                                                                                                                                                                    ttGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTTGGAATGGAAAAAAATGCAAGCAAAAATATAATAAATGCCTAAATTCTTTGAAAATCATTAAACATATCATCATCCTCTATTTTTCAGAATCCGTCGACTTTTAATCACCCGAACGGCAAATTCGGACGGGGGTGCGGACGCTTTGGCCCCCAACCCTCCCGTAAACTGTGTTGATGTTCGTTGATTTTCGAGCCAAAATct                                                     >  1:1003496/1‑238 (MQ=255)
                                                                                                                                                                                                 cgtgaaTTAATGGCGGGTTCTTTTAGGAATAAAAAAAAATACAAGAAATAATAAAATAAGGGCCTAATTTCTTGAAACATCATTAAGCATATCATCATCGTCTATTTGCCAGATTCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGGGGCCAAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:757557/234‑1 (MQ=255)
                                                                                                                                                                                                 cTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:610994/239‑1 (MQ=255)
                                                                                                                                                                                                 cTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGAATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTAAAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCAAAAACTTCCCAGAAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:887807/239‑1 (MQ=255)
                                                                                                                                                                                                 cTGGGATTAATGGCGGGTGCTTTTAGAAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTCCCGAGCCATAATCGAAAAGGCGCGGAt                                        <  1:516179/239‑1 (MQ=255)
                                                                                                                                                                                                                              aaTGAAAAAAAATGCAAGAAATAATAAAATAATGGATTAATTTCTTTCAAATTCATAAAACCTATCAACATCGTCTAGTGGCCAGACTCCTACGTATTATAAGCGCAAGAACAGCATATCAGGACGGTGTTGCGAACGCTGTGGCCAAAAACGTTCGAGTAAACTGGATAGCTGTTCGTAGATTTCCGAGCCATAAACGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAACCTg              <  2:1067348/238‑1 (MQ=255)
                                                                                                                                                                                                                               aTGAAATAAAATGAAAGAAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATTTAATCCTCTTTTATTGGCCAGAATCCGTCGCCTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCAAAAACGTCCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTa            <  2:923273/239‑1 (MQ=255)
                                                                                                                                                                                                                                tGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTCATCTGTac           >  2:607856/1‑238 (MQ=255)
                                                                                                                                                                                                                                tGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTaa           >  2:500947/1‑239 (MQ=255)
                                                                                                                                                                                                                                          aaTGCAAGAAAAAATATAATAATGGCCTATTTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTGGCCAGAATCCGTCGACTTAAAATCGCCAGAACGGCATATAAGGCCGGTGCTGCGAACGCTGTGGCCCAAAACTTTCAAGAAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATaac   <  2:396881/239‑1 (MQ=255)
                                                                                                                                                                                                                                           aTGCAAGAAATAATATAATAATGGCCTATTTCCTTTGAACATCATTAACCATATCATCATCGTCTATTGGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCAAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATaaca  <  2:787183/239‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                            
AAAAAATGAAATAAAGGCAATATATGGTAGTCTGGATCAACGTCAGCTTTAAATGTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGCCAGAACGGCATATCAGGACGGTGCTGCGAACGCTGTGGCCCAAAACGTTCCAGTAAACTGTGTAGATGTTCGTAGATTTCCGAGCCATAATCGAAAAGGCGCGGATGTCTATTAAGGCATCCGGCTAATCTGTAAGCAATAACA  >  NC_000913/4535981‑4536450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: