BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,544	G→T	10.5%	A271S (GCT→TCT)	thrC →	L‑threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544	0	G	T	10.5%	470.1 / 10.8	157	A271S (GCT→TCT)	thrC	L‑threonine synthase
Reads supporting (aligned to +/- strand): ref base G (26/113); new base T (16/2); total (42/115)
Fisher's exact test for biased strand distribution p-value = 5.88e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

GGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTAC  >  NC_000913/4309‑4777

ggTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAGAGGAC‑CCGGGCTGCCGGGGGAACGTTTTAttgttg                                                                                                                                                                                                                                        >  2:1421263/1‑239 (MQ=255)

 gTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCCGGGAGACGTTTTAttgttg                                                                                                                                                                                                                                        >  2:1003762/1‑238 (MQ=255)

     aGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                                   <  1:109679/239‑1 (MQ=255)

     aGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                                   <  1:1228452/239‑1 (MQ=255)

         ggCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTGCTTTGAAGCGGTTGCGCAGCTGCCGCAGGCGACGCGCAACCAGCTGGTTGTCACGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTATCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  2:95596/238‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:672538/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:764167/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:913578/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:1522326/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  2:1207342/236‑1 (MQ=255)

               tgatgatgaAGAGCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                         <  2:1025045/239‑1 (MQ=255)

                     tgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCCTGCGACCGCAC‑TCGGTCTGCCGGGGAAAAGATTTATTGTTGCGCCCCACGTGAACGaacacc                                                                                                                                                                                                                   >  2:206604/1‑234 (MQ=255)

                     tgaAGAACTGAAAGTGGCGCTAGGGTTAAACCCGGCTACCTCGACTACCAACAGCTGTTTGCTGCAGCAGATTTGCTACTACTTTGAAGATGTCGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGTGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCa                                                                                                                                                                                                                                  <  2:1426505/224‑1 (MQ=255)

                      gaagaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGt                                                                                                                                                                                                                  <  1:124942/239‑1 (MQ=255)

                        agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                                <  1:710175/239‑1 (MQ=255)

                        agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                                <  1:861410/239‑1 (MQ=255)

                            cTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTTTGCTGGCCAAGTCACGCCGCTCT‑CCGGGGAAGCGGATTTTTGCTGCGCACAACCTGGACGATACCCTGCCAc                                                                                                                                                                                                             >  2:806726/1‑238 (MQ=255)

                               aaaGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCCGCTTGCGCAGTCCC‑TCGGTCTGGCGAGGAAACGTTTTATTTCTTGGCCCCACCTGAACGa                                                                                                                                                                                                                        >  1:1426505/1‑224 (MQ=255)

                                    ggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGTCAC‑TCGGCCTGCCGGTGAAAAGTTTTTTTGCTGCGCGCAACGGGAACGATACCGGGCCACGTTTCCtt                                                                                                                                                                                                     >  2:574032/1‑237 (MQ=255)

                                      cgcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGACAA‑TCGGTCTGCCGGGGGAACGTTTTTTTGCTGCGACCCACGTGAACGCTACCGTGCCACCTTTCCTGCa                                                                                                                                                                                                   >  1:748200/1‑238 (MQ=255)

                                      cgcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGTCAC‑TCGGGCTGCCGGTGAAACGGTTTATTTCTGCGACCAACGTGAACGATACCGTGGCACCGTTTCTGCac                                                                                                                                                                                                  >  1:927205/1‑239 (MQ=255)

                                       gttAGGTTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTTGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  2:932390/237‑1 (MQ=255)

                                       gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  1:1022823/239‑1 (MQ=255)

                                       gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  1:153346/239‑1 (MQ=255)

                                        cTAGGGTTAACCCCGGCTAACTCGATTACCATCAGCGGTTTGCTGGGGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  2:1228447/238‑1 (MQ=255)

                                              ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           <  2:1337234/238‑1 (MQ=255)

                                              ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           <  2:1221748/238‑1 (MQ=255)

                                               tAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAg                                                                                                                                                                                         <  2:442947/239‑1 (MQ=255)

                                                   cTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                                                                                                                                                                                     <  1:1011034/239‑1 (MQ=255)

                                                      ggCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           >  1:747595/1‑230 (MQ=255)

                                                       gCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCTGGCGAAGTCAC‑CCGGTCTTCCGGTGAAAAGTTTTTTTTCTGCGACCAACGTGAAAGATACCAGGCCCCCGTTCCTCCACCACGGTcgcgtggcaa                                                                                                                                                                                  >  2:1347245/1‑229 (MQ=255)

                                                                 ttAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCa                                                                                                                                                                                                                                  <  1:302832/180‑1 (MQ=255)

                                                                 ttAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       <  1:728520/239‑1 (MQ=255)

                                                                     cATCTGCCGTGTGCTGGCGCAGAGTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                                   <  2:1079406/239‑1 (MQ=255)

                                                                     cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                                   <  1:545076/239‑1 (MQ=255)

                                                                     cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                                   <  1:136076/239‑1 (MQ=255)

                                                                     cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                                   <  1:500799/239‑1 (MQ=255)

                                                                          gCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTTTTTCTGCGACCAACGTGAACGCTACCGGGCCACGTTTCCTGCACGACGGTCAGTGGGCACCCACAGCGACCCAGGCGCCg                                                                                                                                                              >  1:576906/1‑239 (MQ=255)

                                                                              tttGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGTTTCTGGGGGAGGCAC‑CCGGTCCGCCGGGGAAACGCTTTATTGCTGGGGCCAACGTGAACGATACCGTGCCCAGTTTCCCTCACGACGGGCCGTGGTCCCCCAAAGCGCTCCAGGCGACGTTAt                                                                                                                                                          >  2:748399/1‑239 (MQ=255)

                                                                               ttGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATc                                                                                                                                                         <  1:504153/239‑1 (MQ=255)

                                                                                tGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATcc                                                                                                                                                        <  1:1386445/239‑1 (MQ=255)

                                                                                tGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGGAGTCAC‑TCGGGCCGCCGGGGAAAAGGTTTATTGGTGGGACCCAAGTGAACGGTAACCTGCCCCGCTTCCTGCCCGACGGGCCGGGGGCCACCAAAACGAACCAGGGGGCGTTGTc                                                                                                                                                         >  2:1477662/1‑238 (MQ=255)

                                                                                                  actactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGt                                                                                                                                      <  1:1209857/239‑1 (MQ=255)

                                                                                                   ctactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTc                                                                                                                                     <  1:59509/239‑1 (MQ=255)

                                                                                                     actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGGCAC‑CCGGTCTGCCGGGGGAACGGTTTTTTTCTGCGACCCACGTGAAAGAAACCGTGCCCCGTTTCCTGCCCGCCCGTCAGGGGGTCCCCAAAACCACTCCAGCGACGTTTTCCAACCCGCTGGACGGGTGTCa                                                                                                                                    >  2:1251321/1‑238 (MQ=255)

                                                                                                     actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAg                                                                                                                                   <  1:1003762/239‑1 (MQ=255)

                                                                                                     acgtggAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAg                                                                                                                                   <  2:201533/234‑1 (MQ=255)

                                                                                                      ctTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTTCTGGCGAAGCCAC‑TCGGTCTGCCGGGGAAAAGTTTTATTTTTTCGACCAACGTGGACGAAAACGTGCCACGTTTCCTGTACGACGGGCAGAGGTCACCCAAAACGGCTCAGGCGGCGTTTACCAACCCGAAgggagtggggacg                                                                                                                                   >  2:275911/1‑227 (MQ=255)

                                                                                                        ttGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCg                                                                                                                                <  1:362254/239‑1 (MQ=255)

                                                                                                        ttGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCg                                                                                                                                <  1:61003/239‑1 (MQ=255)

                                                                                                                gTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTg                                                                                                                        >  1:740642/1‑239 (MQ=255)

                                                                                                                   gcgcAGCTGCCGCAGGAGACGCGCAACCAGCTTGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGcc                                                                                                                     <  1:173860/239‑1 (MQ=255)

                                                                                                                   gcgcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGcc                                                                                                                     <  2:33458/239‑1 (MQ=255)

                                                                                                                     gcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTTCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCcgc                                                                                                                   <  1:275911/239‑1 (MQ=255)

                                                                                                                     gcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCcgc                                                                                                                   <  2:206753/239‑1 (MQ=255)

                                                                                                                           gCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGGGGAACGTTTTTTTGCTGCGACCAACGTGAAAGATACCGTGCCACGTTTTCTGCACGACGGGCAGTGGTCACCCAAAGCGAACCCGGCGGCGTTATCCAAACCGACGGGCGTGAGTCAGCCGcacaacagcacccgcgtgg                                                                                                             >  2:965964/1‑226 (MQ=255)

                                                                                                                                      cgcgCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTc                                                                                                  <  2:1299399/239‑1 (MQ=255)

                                                                                                                                                       ctaccggcgccaacgggaacggtCGGCGTTTTGACGGCGGGCCTGCGGGCAGAGTCAC‑TCGGTCTCCCGGTAAATCGTTTAATTGCTGCGACCAACGTGAACTATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGGGACTCAGGCGACGTTATCCAACGCGATGGACTTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGc                                                                                  <  2:463609/216‑1 (MQ=255)

                                                                                                                                                            ggggCCAAGCGGAACCTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            <  2:927205/236‑1 (MQ=255)

                                                                                                                                                            ggTGCCAAGCGGAAACTTCGGCGATTTGCCGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            <  1:373783/239‑1 (MQ=255)

                                                                                                                                                            ggTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            <  1:778524/239‑1 (MQ=255)

                                                                                                                                                                 gaaGCGGAAACTTCGGCGAGTTGACGGCGGGTCTGCTGGCGATGTAAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACTTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  2:681493/238‑1 (MQ=255)

                                                                                                                                                                 cAAGCGGAACCTTCGGCGATTTGACGGCGGGTGTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAATGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  2:113142/239‑1 (MQ=255)

                                                                                                                                                                 cAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  1:1347245/239‑1 (MQ=255)

                                                                                                                                                                   aGCGGAACCTTCGGCGTTTGGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCt                                                                     <  2:143896/239‑1 (MQ=255)

                                                                                                                                                                   aGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCt                                                                     <  1:965964/239‑1 (MQ=255)

                                                                                                                                                                       gAACCTTCGGAGAGTTGGCGGCGGGTCTGCTGGCGGAGTCAC‑TGGGTCGGCCGGTGAAACCTTTTATTGCGGCAGCCAACGTGAACGATACCGTGCAGGGTTGCCTGCACGGCGTGCAGTGGTCACCCAAAGCGACTCAGTCGACGTTATCCAACGCGTTGGACGTGAGTCAGCCGAACAACTGGCCGCTTGTGGAAGCGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTggg                                                                  <  2:355700/238‑1 (MQ=255)

                                                                                                                                                                       gAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTggg                                                                  <  1:578111/238‑1 (MQ=255)

                                                                                                                                                                           cTTCGGCGATTTGACGGGGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                             <  2:854504/239‑1 (MQ=255)

                                                                                                                                                                           cTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGACCC‑TCGGTCTGCCGGTAAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                             <  2:506498/239‑1 (MQ=255)

                                                                                                                                                                                  gATTTGACGGGGGGTCTGCTGGCGAAGTTAC‑TCGGTCTGCCGGTCAAACGTTTTATTGCCGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGCCTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                                       <  2:259970/238‑1 (MQ=255)

                                                                                                                                                                                  gATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAATCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                                       <  1:1288822/238‑1 (MQ=255)

                                                                                                                                                                                    ttggagggcggtgttgctgGCGAAGGCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATGCCGGGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTg                                                     <  2:288478/224‑1 (MQ=255)

                                                                                                                                                                                            cggGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATGGCTGCGACCAACGTGAACGATACCGGGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGAtgatga                                             <  1:811895/238‑1 (MQ=255)

                                                                                                                                                                                                gccgctgGCGAAGTCCC‑CCGGTCTGCCGGTGAAACGTTTTATTGCTGCGCCCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACGCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                                         <  2:821767/235‑1 (MQ=255)

                                                                                                                                                                                                 ctgctgGCGAAGTCAC‑ACGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCa                                        <  1:905677/238‑1 (MQ=255)

                                                                                                                                                                                                  tgctgGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACTTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            <  1:667039/201‑1 (MQ=255)

                                                                                                                                                                                                  tgctgGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGGGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCAc                                       >  2:1518295/1‑238 (MQ=255)

                                                                                                                                                                                                  tgctgGCGAAATCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGGGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTTGGTTATGCAGCCGTGGATGAAGAAACCAc                                       >  2:984771/1‑238 (MQ=255)

                                                                                                                                                                                                     tgGCGAAGTCCC‑TCGGTCTGCCGGGGAAACGTTTTATTGCGGCGACCAACGGGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCCCCCAAAGCGATTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCa                                    <  2:254095/238‑1 (MQ=255)

                                                                                                                                                                                                     tgGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                                   <  1:803635/239‑1 (MQ=255)

                                                                                                                                                                                                     tgGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                                   <  2:740642/239‑1 (MQ=255)

                                                                                                                                                                                                          aaGTCAC‑TCGGTCTGCCGGTGAACCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGa                               <  1:212705/238‑1 (MQ=255)

                                                                                                                                                                                                           aGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACa                             <  2:548364/239‑1 (MQ=255)

                                                                                                                                                                                                                c‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  1:1063755/239‑1 (MQ=255)

                                                                                                                                                                                                                c‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  1:1107840/239‑1 (MQ=255)

                                                                                                                                                                                                                c‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  1:999227/239‑1 (MQ=255)

                                                                                                                                                                                                                 cacgggcgcccGGTGAAACGTTGTATGTCTGCGCCCAACGTGACCGATACCGTGCCACGTTTCCAGCACGACGGTCAGTGGTCCCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  2:748200/230‑1 (MQ=255)

                                                                                                                                                                                                                  tCGGTCTGCCGGTGAAACTTTTTATTGCGGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCCCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt                       <  2:1265778/239‑1 (MQ=255)

                                                                                                                                                                                                                  tCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt                       >  2:1500253/1‑239 (MQ=255)

                                                                                                                                                                                                                      tCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       >  1:203107/1‑91 (MQ=255)

                                                                                                                                                                                                                              tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       >  2:671617/1‑83 (MQ=255)

                                                                                                                                                                                                                              tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  1:743729/1‑238 (MQ=255)

                                                                                                                                                                                                                               gAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGaa           <  1:178708/238‑1 (MQ=255)

                                                                                                                                                                                                                               gAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGaa           <  1:1251321/238‑1 (MQ=255)

                                                                                                                                                                                                                                aaaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  2:169575/1‑236 (MQ=255)

                                                                                                                                                                                                                                aaaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  2:295628/1‑236 (MQ=255)

                                                                                                                                                                                                                                 aaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTg        <  1:1092119/239‑1 (MQ=255)

                                                                                                                                                                                                                                    gTTTTATTGCTGCGACAAACATGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCAAAAGAGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGc     <  1:900835/239‑1 (MQ=255)

                                                                                                                                                                                                                                        tATTGCGGCGCCCAACGGCAACGATACCTTGCCACGTTTCCTGCACGCCGGTCAGGGGTCACCCAAAGCACCTCAGGCGCCGTTATCCAACGCGATGGACTTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTa   <  2:1182978/237‑1 (MQ=255)

GGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCAC‑TCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTAC  >  NC_000913/4309‑4777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`