BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,022,938	T→C	2.8%	Y16H (TAC→CAC)	fliR →	flagellar export pore protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,938	0	T	C	2.8%	527.7 / 2.7	147	Y16H (TAC→CAC)	fliR	flagellar export pore protein
Reads supporting (aligned to +/- strand): ref base T (82/60); new base C (2/2); total (85/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-01

Only 100 of 154 total aligned reads displayed.

TGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAATTTGCCTTTGCCGCTGT  >  NC_000913/2022701‑2023176

tGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGt                                                                                                                                                                                                                                                >  2:1032368/1‑238 (MQ=255)

  gTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACt                                                                                                                                                                                                                                             >  1:620268/1‑239 (MQ=255)

    cACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACtt                                                                                                                                                                                                                                            <  2:654657/238‑1 (MQ=255)

    accGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACtt                                                                                                                                                                                                                                            <  2:94020/236‑1 (MQ=255)

       gggCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGTGAACAATGGCTATCCTGGTTAAACCTGAACTTCTg                                                                                                                                                                                                                                         >  2:1155758/1‑238 (MQ=255)

       gggCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTgg                                                                                                                                                                                                                                        <  1:362510/239‑1 (MQ=255)

            ttATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGtt                                                                                                                                                                                                                                   >  1:1325843/1‑239 (MQ=255)

                   caGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTgcgc                                                                                                                                                                                                                            >  1:857789/1‑239 (MQ=255)

                   caGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTgcgc                                                                                                                                                                                                                            >  2:656882/1‑239 (MQ=255)

                       aTTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGc                                                                                                                                                                                                                        <  2:194424/239‑1 (MQ=255)

                           tGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGgc                                                                                                                                                                                                                    >  2:267026/1‑239 (MQ=255)

                           tGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGAAGCCGTACTATGTTGCAGGTGCCAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGAGCTGgc                                                                                                                                                                                                                    >  2:1375018/1‑239 (MQ=255)

                            gCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGgcg                                                                                                                                                                                                                   <  1:610235/239‑1 (MQ=255)

                                gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGa                                                                                                                                                                                                               >  2:1095407/1‑239 (MQ=255)

                                gccgccACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGa                                                                                                                                                                                                               >  1:282183/1‑239 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGgcg                                                                                                                                                                                                                   >  2:276384/1‑213 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGgcg                                                                                                                                                                                                                   <  1:276384/213‑1 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCAc                                                                                                                                                                                                        >  1:4207/1‑224 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCAc                                                                                                                                                                                                        <  2:4207/224‑1 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGa                                                                                                                                                                                          >  1:462977/1‑238 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGa                                                                                                                                                                                          >  2:363242/1‑238 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGa                                                                                                                                                                                          >  1:1134220/1‑238 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGa                                                                                                                                                                                          >  1:667550/1‑238 (MQ=255)

                                                      tGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGa                                                                                                                                                                                          >  1:745853/1‑238 (MQ=255)

                                                          gCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATATCCACCGCGCCGATTCTGAGCGa                                                                                                                                                                                      >  2:365442/1‑238 (MQ=255)

                                                             gTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGc                                                                                                                                                                                  >  2:680471/1‑239 (MQ=255)

                                                                        cGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGaa                                                                                                                                                                       <  2:1277496/239‑1 (MQ=255)

                                                                                    ccGTATTTATCGTATCTAATATTGCCGGAGCGGGGATTCTCAGTCTGTTGTTAGATTCGCTCCGCACTTTGTTCGCTAACCAGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGGGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACt                                                                                                                                                           <  2:11674/239‑1 (MQ=255)

                                                                                    ccGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACt                                                                                                                                                           <  2:1325843/239‑1 (MQ=255)

                                                                                     cGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACt                                                                                                                                                           <  1:135625/238‑1 (MQ=255)

                                                                                       tATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTggg                                                                                                                                                        >  2:434555/1‑239 (MQ=255)

                                                                                                    tattattGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAatgatg                                                                                                                                           <  1:1487867/239‑1 (MQ=255)

                                                                                                           gCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGt                                                                                                                                                                 >  2:1197217/1‑210 (MQ=255)

                                                                                                           gCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGt                                                                                                                                                                 <  1:1197217/210‑1 (MQ=255)

                                                                                                             cGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACGATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGtt                                                                                                                                   <  1:567753/238‑1 (MQ=255)

                                                                                                              ggACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCTCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGtt                                                                                                                                   <  1:656719/239‑1 (MQ=255)

                                                                                                              ggACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATATCTCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGtt                                                                                                                                   <  1:1122173/239‑1 (MQ=255)

                                                                                                                aCCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCCAGCCGATTCTTAGCGAACGCTGCGTACCGAAACGGGTAACACTGCGTCTGGCCATCATGGACACGTTcac                                                                                                                                >  2:400617/1‑236 (MQ=255)

                                                                                                                    tGGATGCTCAATCTGTTGCTGGATTACGTCCGCCCCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCAtt                                                                                                                            >  1:1224248/1‑238 (MQ=255)

                                                                                                                    tGGAGGCTCAATCGTTTGGTGGATTACGACCGCTCCTGTTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCTGTACTATGTTGCAGGTGACAAGCGAACAATGGCTCTCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTg                                                                                                                           <  2:249317/239‑1 (MQ=255)

                                                                                                                     ggATGCTCAATCTGTTGCTGGATTACGTGCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGc                                                                                                                          <  2:146098/239‑1 (MQ=255)

                                                                                                                       aTGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGcc                                                                                                                         <  2:422946/238‑1 (MQ=255)

                                                                                                                          cTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGc                                                                                                                                                                                        >  1:967487/1‑172 (MQ=255)

                                                                                                                          cTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGc                                                                                                                                                                                        <  2:967487/172‑1 (MQ=255)

                                                                                                                            cAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCcatca                                                                                                                   >  2:828006/1‑239 (MQ=255)

                                                                                                                              aTCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCAtt                                                                                                                 >  2:742868/1‑239 (MQ=255)

                                                                                                                                   ttGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCt                                                                                                             >  1:299380/1‑238 (MQ=255)

                                                                                                                                         gATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCg                                                                                                                                                                              <  1:329708/167‑1 (MQ=255)

                                                                                                                                         gATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCg                                                                                                                                                                              >  2:329708/1‑167 (MQ=255)

                                                                                                                                             aCGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGCTCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGTCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCACCGCTGt                                                                                                  >  2:860903/1‑239 (MQ=255)

                                                                                                                                             aCGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGcc                                                                                                                         >  1:1445699/1‑216 (MQ=255)

                                                                                                                                                   gCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGCAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCTCATCATTACCTGCCAACGATGTTCCTGt                                                                                            >  1:27426/1‑239 (MQ=255)

                                                                                                                                                    cACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGtt                                                                                           >  2:60223/1‑239 (MQ=255)

                                                                                                                                                              aCTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCt                                                                                 <  1:380887/239‑1 (MQ=255)

                                                                                                                                                               cTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCtt                                                                                <  1:680471/239‑1 (MQ=255)

                                                                                                                                                                 aaCCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt                                                                               >  2:1062579/1‑238 (MQ=255)

                                                                                                                                                                 aaCCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCttt                                                                               >  2:168272/1‑238 (MQ=255)

                                                                                                                                                                   ccTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGct                                                                            <  1:1032368/239‑1 (MQ=255)

                                                                                                                                                                   ccTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGct                                                                            <  1:828006/239‑1 (MQ=255)

                                                                                                                                                                     tGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGc                                                                             >  1:795476/1‑236 (MQ=255)

                                                                                                                                                                     tGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGc                                                                             >  2:711554/1‑236 (MQ=255)

                                                                                                                                                                       ccGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCa                                                                                                                                                                                 <  1:272293/134‑1 (MQ=255)

                                                                                                                                                                       ccGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCa                                                                                                                                                                                 >  2:272293/1‑134 (MQ=255)

                                                                                                                                                                       ccGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTc                                                                                                                                                      <  1:1262401/161‑1 (MQ=255)

                                                                                                                                                                       ccGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCtgt                                                                        >  1:429037/1‑239 (MQ=255)

                                                                                                                                                                            ta‑‑tCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTc                                                                                      <  2:676144/220‑1 (MQ=255)

                                                                                                                                                                             a‑‑tcatcGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGtggctgg                                                                  <  1:434555/239‑1 (MQ=255)

                                                                                                                                                                                   tcGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGCTCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGt                                                              >  1:591224/1‑239 (MQ=255)

                                                                                                                                                                                   tcGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTAc                                                                                                               <  1:293999/190‑1 (MQ=255)

                                                                                                                                                                                   tcGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGt                                                              >  1:1086775/1‑239 (MQ=255)

                                                                                                                                                                                   tcGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGt                                                              >  1:1263846/1‑239 (MQ=255)

                                                                                                                                                                                      ggTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTgca                                                           >  2:1321689/1‑239 (MQ=255)

                                                                                                                                                                                        tAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTgcagc                                                         >  1:452927/1‑239 (MQ=255)

                                                                                                                                                                                           ccGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGtt                                                                                                                                   >  1:802945/1‑162 (MQ=255)

                                                                                                                                                                                           ccGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGa                                                      >  1:1460/1‑239 (MQ=255)

                                                                                                                                                                                                      tGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGc                                           >  1:1339467/1‑239 (MQ=255)

                                                                                                                                                                                                       gCAGGTGACAAGCGAACAATGGCTTTCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCa                                          <  2:1176133/239‑1 (MQ=255)

                                                                                                                                                                                                       gCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCa                                          <  2:620268/239‑1 (MQ=255)

                                                                                                                                                                                                        cAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGAGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCTGCGTACCGCAACGGGTAAAACTGGGTCTGGCAATGATAATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCAt                                         >  2:774642/1‑239 (MQ=255)

                                                                                                                                                                                                         aGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCAt                                         <  2:1157253/238‑1 (MQ=255)

                                                                                                                                                                                                         aGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCAt                                         <  1:860903/238‑1 (MQ=255)

                                                                                                                                                                                                             gACCAGCGAAACATGGCTATCCTTGTTAAGCCTTTGCTTCTGGCCGTTGCTGCGCGTGCTGGCGCGGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATTATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgcgc                                    <  2:823498/239‑1 (MQ=255)

                                                                                                                                                                                                             gACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCCCCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgcg                                     <  2:1386565/238‑1 (MQ=255)

                                                                                                                                                                                                             gACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgcg                                     <  2:795476/238‑1 (MQ=255)

                                                                                                                                                                                                             gACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgcg                                     <  2:282183/238‑1 (MQ=255)

                                                                                                                                                                                                             gACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgc                                      >  2:1140430/1‑237 (MQ=255)

                                                                                                                                                                                                               cAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCt                                   <  1:1095407/238‑1 (MQ=255)

                                                                                                                                                                                                                    gAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGttt                             >  2:820735/1‑239 (MQ=255)

                                                                                                                                                                                                                             cTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGct                                                                            >  1:745792/1‑183 (MQ=255)

                                                                                                                                                                                                                             cTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGct                                                                            <  2:745792/183‑1 (MQ=255)

                                                                                                                                                                                                                                     gTTAAACCTGCACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgcg                                     >  2:823556/1‑214 (MQ=255)

                                                                                                                                                                                                                                     gTTAAACCTGCACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTgcg                                     <  1:823556/214‑1 (MQ=255)

                                                                                                                                                                                                                                      ttAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAAtttgcctt           >  2:22232/1‑239 (MQ=255)

                                                                                                                                                                                                                                          aCCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAAtttgcctttgcc       >  2:455993/1‑239 (MQ=255)

                                                                                                                                                                                                                                          aCCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAAtttgcctttgcc       >  1:246154/1‑239 (MQ=255)

                                                                                                                                                                                                                                              gTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAATTTGCCTTTGCCGCtg   <  2:429037/239‑1 (MQ=255)

                                                                                                                                                                                                                                               tACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGt                                                              >  2:455270/1‑179 (MQ=255)

                                                                                                                                                                                                                                               tACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAATTTGCCTTTGCCGCtgt  >  1:1145796/1‑239 (MQ=255)

                                                                                                                                                                                                                                               tACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAATTTGCCTTTGCCGCtgt  >  2:335314/1‑239 (MQ=255)

                                                                                                                                                                                                                                               tACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAATTTGCCTTTGCCGCtgt  >  1:478135/1‑239 (MQ=255)

TGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACGCAGATTAACGAAATGACGCTGTCGTTTATTCCGAAAATCATCGCCGTATTTATCGCCATTATTATTGCCGGACCGTGGATGCTCAATCTGTTGCTGGATTACGTCCGCACCTTGTTCACTAACCTGCCGTATA‑‑TCATCGGGTAGCCGTACTATGTTGCAGGTGACAAGCGAACAATGGCTATCCTGGTTAAACCTGTACTTCTGGCCGTTACTGCGCGTGCTGGCGCTGATCTCCACCGCGCCGATTCTGAGCGAACGCAGCGTACCGAAACGGGTAAAACTGGGTCTGGCAATGATGATCACGTTCGCCATTGCCCCATCATTACCTGCCAACGATGTTCCTGTTTTTTCGTTCTTTGCTCTGTGGCTGGCCGTGCAGCAGATCCTGATCGGCATTGCGCTTGGTTTTACCATGCAATTTGCCTTTGCCGCTGT  >  NC_000913/2022701‑2023176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`