BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,361,311	A→T	13.3%	N34K (AAT→AAA)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,311	0	A	T	13.3%	267.5 / 2.3	101	N34K (AAT→AAA)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand): ref base A (45/39); new base T (2/13); total (48/53)
Fisher's exact test for biased strand distribution p-value = 4.55e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACC  >  NC_000913/2361075‑2361549

tCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGAtt                                                                                                                                                                                                                                                 <  2:1328828/239‑1 (MQ=255)

         aGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTg                                                                                                                                                                                                                                        >  1:687899/1‑239 (MQ=255)

            ttGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTgg                                                                                                                                                                                                                                       <  2:1069454/237‑1 (MQ=255)

            ttGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTGCTGCTT‑CGACTTCAACAGCCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑c                                                                                                                                                                                                                                    <  2:486292/239‑1 (MQ=255)

            tggATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCTACAATAAAACAGCCCATTTCACCGGCTGTAGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑c                                                                                                                                                                                                                                    <  2:244958/237‑1 (MQ=255)

             tGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑Cg                                                                                                                                                                                                                                   >  2:745617/1‑239 (MQ=255)

                     cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑Tc                                                                                                                                                                                                                          >  1:1093884/1‑239 (MQ=255)

                     cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑Tc                                                                                                                                                                                                                          >  2:1291300/1‑239 (MQ=255)

                         cacaTTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGAtt                                                                                                                                                                                                                                                 >  1:653101/1‑214 (MQ=255)

                         cacaTTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGAtt                                                                                                                                                                                                                                                 <  2:653101/214‑1 (MQ=255)

                              ttACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCgccgcc                                                                                                                                                                                                                 <  2:206799/239‑1 (MQ=255)

                               tACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCa                                                                                                                                                                                                                <  1:1491606/239‑1 (MQ=255)

                                 cccTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAgc                                                                                                                                                                                                              <  1:932412/239‑1 (MQ=255)

                                  ccTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTgg                                                                                                                                                                                                                                       >  1:1416580/1‑215 (MQ=255)

                                  ccTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTgg                                                                                                                                                                                                                                       <  2:1416580/215‑1 (MQ=255)

                                  ccTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAgcg                                                                                                                                                                                                             <  1:136827/239‑1 (MQ=255)

                                  ccTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGGGATAA‑TCACCGCCGCCAgc                                                                                                                                                                                                              >  2:423692/1‑238 (MQ=255)

                                  ccTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATACAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAgc                                                                                                                                                                                                              >  1:159161/1‑238 (MQ=255)

                                    tCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAgcg                                                                                                                                                                                                             >  1:1147276/1‑237 (MQ=255)

                                      aaTGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGTCTTCAACAACCAACGTGCCAAGAACGGTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGGAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCATTGGTTCGCCCCCTGGT‑CGTGATTA‑TCACCACCGCCAGCGCCtt                                                                                                                                                                                                         >  1:73976/1‑239 (MQ=255)

                                      aaTGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCtt                                                                                                                                                                                                         >  2:1515047/1‑239 (MQ=255)

                                      aaTGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCtt                                                                                                                                                                                                         >  1:815707/1‑239 (MQ=255)

                                      aaTGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCtt                                                                                                                                                                                                         >  1:301096/1‑239 (MQ=255)

                                      aaTGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGAATTTACT‑CATCGGGGTGGCAATTTGATCGTCTATCCAGGGATTCCCCCCCTGTT‑CGTGATAA‑TCACCGCCCCCCGCCCCtt                                                                                                                                                                                                         >  1:1261456/1‑239 (MQ=255)

                                           aaCGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTct                                                                                                                                                                                                    >  1:1402746/1‑239 (MQ=255)

                                           aaCGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTc                                                                                                                                                                                                     <  1:745617/238‑1 (MQ=255)

                                            aCGGTTAAGATGTTTTTCGACAATAAAACCGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGCTCTGGCTGTTTTCTGCTT‑CGACATCAACCACCAACCCTCCAAGAACGTTCATGCCATA‑TGCCTGGCG‑TTAACG‑CTCGAAATCGCGGGATTTACT‑GTTTGGGGCGGTAAATTGGTCGTCTATCCAATCTCTCGCCCCCTGGT‑GGCGATAA‑GCTCCCCTCCCAGCGCCTTTTTctg                                                                                                                                                                                                   >  2:762260/1‑239 (MQ=255)

                                                ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTct                                                                                                                                                                                                    >  2:274534/1‑234 (MQ=255)

                                                ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTct                                                                                                                                                                                                    >  1:270702/1‑234 (MQ=255)

                                                ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTct                                                                                                                                                                                                    >  1:1341756/1‑234 (MQ=255)

                                                ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTct                                                                                                                                                                                                    >  1:197806/1‑234 (MQ=255)

                                                ttAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CACGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTCTCCAGTGATTTGCCCCCTGGT‑CGTGGTAA‑ACCCCCCCGCCGGCGCCTTTTTcc                                                                                                                                                                                                    >  2:413355/1‑233 (MQ=255)

                                                     attttttcCAACAAAAAACCCGCCCATTCCCCGGCCTGTCGAACCGGCGAATCCGGTCTGGCCGTTTTCTGTTG‑CTACTCAACCACCCACCGTGCAAAGAACGTAAAGGCAAAA‑TGCCGGGCG‑TGTCTC‑CTCGTAACCGCGTTTTTTACC‑CAGCGGGGTGGCAATATGACCGTCTACCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                                                                                                                                                          <  2:833053/236‑1 (MQ=255)

                                                      tGTTTTTCAAAAAAAAAACAGCCCATTCCCCCGGCTTCCGAAACGGCGAATCCGGTCTGCCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAACGCCAAA‑TGACTGGCG‑TGACTG‑CCCGTAACCGCGGTATTTCCC‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATCCGCCCCCTGGT‑CGTGATAA‑TCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCAcc                                                                                                                                                                                         <  2:153791/239‑1 (MQ=255)

                                                                      aaCAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGgcg                                                                                                                                                                         <  1:864750/239‑1 (MQ=255)

                                                                               tttCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTATT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGt                                                                                                                                                                >  1:530983/1‑239 (MQ=255)

                                                                                ttCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGtt                                                                                                                                                               >  1:168886/1‑239 (MQ=255)

                                                                                     tgccTGGCGAAACGGCACACCGGGTCTGGCCGTTTTCTGCTC‑CGACGTCAACACCAAACGTCCCAAGAGCG‑TAAAGCCAAATTGCCTGG‑G‑TGGCCGCCTGGAAACCGGGTTATTTCCC‑CACCGGGGGGCAAATATGACCGTCTCCCCGGTGTTCCGCCCCCTGGC‑CGTGAAAA‑CCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑tt                                                                                                                                                         <  2:1261456/236‑1 (MQ=255)

                                                                                     cGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑tt                                                                                                                                                         >  1:126848/1‑239 (MQ=255)

                                                                                           tCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGt                                                                                                                                                   >  1:501627/1‑239 (MQ=255)

                                                                                               aagggCGAGTCCGTCCTGGCCGTTTTCGGCGT‑CGCCTTCAACCGCAACCGTCCCAAGAACTTTAATGCCAAA‑TGCGTGGCG‑TGCCTG‑CTCGTACGCGCGTTATTTACG‑CATCGGGGTGTCAATATGTCCGCCTATCCAGTGACTCCCCCCTTGGT‑CGTGATAA‑CCCCGGCCGCCAGCGCTTTTTTTTGTTGTCGATCCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAggg                                                                                                                                               <  2:339567/236‑1 (MQ=255)

                                                                                                    caaaacgtgTCTGCCAGTTTTTCCCTT‑CGACCCAAACAACCAACGGCCCAGAAACGTAAATGCCAAA‑TTGCGGGCT‑TGCCTC‑CTCGTACCCGTGGTATTTCCT‑CACGGGGGGGGAAATATGTCCGTTTCTCCGGTGATCCGCCCCCTGGT‑CGTTATAA‑CCCCCGCCGCCAGCCCTTTTTTCTGTTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCat                                                                                                                                           <  2:159161/230‑1 (MQ=255)

                                                                                                     gAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTTGGTAGGGTCatg                                                                                                                                          >  1:709437/1‑239 (MQ=255)

                                                                                                         ctggTCTGCCCGTTTTCTGTTC‑CGACTCAACCAACCACCGTGCCAAGAACGTTAATCCCAAA‑TGACTGGCG‑GGCTTG‑TTCTAAACCGCGTTATTTACT‑CACGGGGGTGGCAATAGGACCGTTTATCCGGTGATTCCCCCCCGGGT‑CGTGAAAA‑TCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGtt                                                                                                                                     <  2:1402746/237‑1 (MQ=255)

                                                                                                         cgggTTTGTCCGTTTTCTGCTC‑CGACTCCAACAACCAACGTCCCAAGAACTTTAATGCAAAA‑TGCCGGGCG‑TGACTG‑CTCGTAACCGGGTTATTTCCT‑CAGCGGGGTGGAAATATGACCTTCTATCCAGTGATCCCCCCCCGGGC‑CGTGATAA‑CCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGtt                                                                                                                                     <  2:815707/237‑1 (MQ=255)

                                                                                                         ccGTTCTAGCCTTTTCCTTCTC‑CGCCTTCAACAACCAACGGGCAAATACCTTTAATGCAAAA‑GGTCTGG‑G‑TTGCTGCCTCTTATTCGGGTTATTTCCT‑CACCGGGGTGAAATTATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGGGATAA‑TCCCCGCCGCCAGCGCTTTTTTCTGTTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGtt                                                                                                                                     <  2:693659/239‑1 (MQ=255)

                                                                                                         ccGGTCTGGCCTTTTTCCGTTT‑CGACTCACACAACAAACGGCCCAAAAAGTTAAAGGCCAAA‑TGAGTGGCG‑TGCTTC‑CTGTAAAGCGGGTTATTTACT‑CAGGGGGGTGGAAATATGTCCGTCTACCCAGTGTTTCGCCCCCGGGT‑CGTAATAA‑CCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGtt                                                                                                                                     <  2:1440076/239‑1 (MQ=255)

                                                                                                             tcggcccGTTTTCTGCTT‑CGACTTCAACACCCAACGGGCCAAGACCGTTAAGCCCAAA‑TGACTGGCG‑TGATTG‑CTGTAAACCGGGTTATTTCCT‑CACCGGGGTGGAAATATGTTCGTCTACCCAGTGTTTCCCCCCCTGGT‑CGTAATAA‑TCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTtctc                                                                                                                                  <  2:270702/233‑1 (MQ=255)

                                                                                                              ggggCCGTTTTCTGTTT‑CGCCTCCAAAAACCACGGTGCAAAGAACGTTAATGCCAAA‑TGACGGGCG‑TGTCTG‑CCCGTAACCGCGTTATTTCCT‑CATGGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGAAAA‑CCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCa                                                                                                                                <  2:197806/237‑1 (MQ=255)

                                                                                                              cTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAgcg                                                                                                                                                                                                             >  2:1430420/1‑163 (MQ=255)

                                                                                                              cTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAgcg                                                                                                                                                                                                             <  1:1430420/163‑1 (MQ=255)

                                                                                                               cgggccttttCCGCTTCCGCCTTCAAAACCCACGGGGCCAGAAACGTTAAGCCAAAA‑TGTCGGGCG‑TGACGG‑CTGTAAATCG‑GGTTTTTTCTCCGCCGGGGTGGAAATATGTTGTTCTACCCAGTGTTCCGCCCCCTGGT‑GGTGATAA‑TCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCa                                                                                                                                <  2:973884/233‑1 (MQ=255)

                                                                                                                       ttCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGa                                                                                                                       >  1:255590/1‑239 (MQ=255)

                                                                                                                            cTT‑CGACTTCAACAACCAACGTGACAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGAGGCAATATGATCGTCTATCCAGTGTTTCGCCCCCTGGG‑CGAGCTAA‑TCACGCCCGCCAGCGCCTTTTTCTGCTGCCGCACAGCCCGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTATCCATTGTTATGCGGCtt                                                                                                                  >  1:623465/1‑239 (MQ=255)

                                                                                                                              t‑CGATTGCAAAAACCAACGTGCCAAAAATGTTAATACCAAA‑TGTGGGGCT‑TGCCTG‑CTCTTAATCGGGTTATTCACC‑CACCGGGGTGGCAATATGTTCTTCTACCCAGTGATTCGCCCCCTGGT‑CGAGATAA‑TCACCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGt                                                                                                                <  2:1052734/239‑1 (MQ=255)

                                                                                                                                       aacaacCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCCTGATGTTCTCCATTGTTCAGAGGCTTGTAAGTCaaa                                                                                                        >  2:813811/1‑239 (MQ=255)

                                                                                                                                           acaacggTGCCAAAACCGTTAAGCCAAAA‑TGACTGGCT‑TGACTC‑CTGTTAACCGCGTTTTTTCCT‑CATGGGGGTGGAAATATGATCGTCTATCCAGTGATTCGCCCCTTGGT‑GGTAATAA‑CCACCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGa                                                                                                    <  2:1113304/233‑1 (MQ=255)

                                                                                                                                            ccaacGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAggg                                                                                                     >  1:118614/1‑237 (MQ=255)

                                                                                                                                                 ggccaaaGAACGTAAATG‑CAAA‑TGGCCTGCGTTGCCTG‑CTCTTAACGGCGGTTTTTTCT‑CATGGGGGGGGCAATAGATTCGTCTTCCCATTGTTCCGCCCCCT‑GT‑CCTTTTAACTCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACttt                                                                                                <  2:638286/232‑1 (MQ=255)

                                                                                                                                                    ccaggactgTTAATACCAAA‑TGACGGGCG‑TGACTG‑CTCGAATGCGTGTTTTTCCCC‑CAGGGGGGGGCCAATTGGAGCTATTATCCGGTGTTCCCCCCCCGGGT‑GGTGATAA‑CCCCCGCCGCCAGCGCTTTTTTCTGCTGGCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCAt                                                                                           <  2:1462890/231‑1 (MQ=255)

                                                                                                                                                    ccAAGACCGTTAATCACAAA‑TGACGGGTG‑GTACTG‑CTCGTAAGCGGGGTATTTCCC‑CATGGGGGGGGCAATATGGTCGTCTACCCATTGATTCCCCCCCTGGG‑CGTGATAA‑TCCCCGCCGCCAGCCCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCAt                                                                                           <  2:168886/239‑1 (MQ=255)

                                                                                                                                                           cttaaaTGCAAAA‑TGACTGCGG‑TGGCTG‑CTCGAAATCGTGTTATTTCCT‑CTTCGGGGTGAAAATATGTCCGTTTACCCAGTTTTTCGCCCCCGGGT‑GGGGAAAA‑CCCCCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACa                                                                                    <  2:459165/235‑1 (MQ=255)

                                                                                                                                                           cGTTAATGCCAAA‑TGACGGGCT‑TGACTG‑CTCGTAACCGCGGTATTTACT‑CAGCGGGGTGGCAATATGATCGTCTCTCCATTGATTCGCCCCCTGGT‑CGGGATAA‑CCCCCGCCGCCAGCTCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACa                                                                                    <  2:1175657/239‑1 (MQ=255)

                                                                                                                                                             ttAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAg                                                                                   >  1:217432/1‑238 (MQ=255)

                                                                                                                                                                            agggcggtggtcg‑CTCGTAATGGGGGTTTTGACC‑CGGGGGGGGGGAAAGATGATGTCCTACCCGGTGTTCCGCCCCTTGGC‑CGTAATAA‑CCCCCGCGGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATcc                                                                      <  2:118614/226‑1 (MQ=255)

                                                                                                                                                                                    tgcctacaaatAATCGGGGTATTTACT‑GCGTGGGGTGGAAATTTGATCGTCTATCAAGTGATCCGCCCCCTGTC‑CGTGATAA‑CCACCGCCGCCAGCGCCTTTTTCTGCTGTCACCCCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGgcgc                                                               <  2:1072295/228‑1 (MQ=255)

                                                                                                                                                                                          gtctgaaTCGGGGTTTTGTCT‑CATCGGGTTGCAAATATTACCGTCTATCCATTGTTTCTCCCCCGGGT‑CGTAAAAA‑TCACCGCCGCAAGCGCCTTTTTTCGCTGGCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTGGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCaa                                                         <  2:1093884/233‑1 (MQ=255)

                                                                                                                                                                                          cTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCCATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTTA‑TTTTTGGTAGGGTCACGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGCCTTTTCCTTCCCAACAGACGTGAAATATCTCTGGCCAAGGAAAt                                                        >  2:862598/1‑239 (MQ=255)

                                                                                                                                                                                           tCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATg                                                       >  1:1263423/1‑239 (MQ=255)

                                                                                                                                                                                                    cgGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGTACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGcc                                               >  2:1159838/1‑238 (MQ=255)

                                                                                                                                                                                                    cgGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGcc                                               >  1:862841/1‑238 (MQ=255)

                                                                                                                                                                                                            aCT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATa                                      >  2:146042/1‑239 (MQ=255)

                                                                                                                                                                                                               tctgcGGGGTGGCAATATGACCGTCTATCCAGTG‑TTCCCCCCCTTGTCCGTAATAA‑CCACCGCCGCCAGCGCTTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAAt                                    <  1:1508101/235‑1 (MQ=255)

                                                                                                                                                                                                                 aTCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCa                                  <  1:274534/239‑1 (MQ=255)

                                                                                                                                                                                                                  tCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCa                                  <  1:454308/238‑1 (MQ=255)

                                                                                                                                                                                                                  tCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCa                                  >  2:454308/1‑238 (MQ=255)

                                                                                                                                                                                                                  tCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCAc                                 >  1:667065/1‑239 (MQ=255)

                                                                                                                                                                                                                  tCGGGGAGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCAc                                 >  1:965327/1‑239 (MQ=255)

                                                                                                                                                                                                                   cGGGGTGGCATTGTACTCTTCTACCCAGTGTTGCGCCCCCGGGT‑CGGGATAA‑TCCCCGCCGCCAGCGCCTTTTTCTGCGGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACg                                <  1:423692/239‑1 (MQ=255)

                                                                                                                                                                                                                    ggggTGGCAATATGATCGTCTATCAAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGTACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCa                                  <  1:1159838/236‑1 (MQ=255)

                                                                                                                                                                                                                    ggggTGGAAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACg                                <  1:1515047/238‑1 (MQ=255)

                                                                                                                                                                                                                       gTGGCAATATGTTCGTCTATCCAGTGTTCCGCCCCCTGGT‑CTTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTa                             <  1:1239077/238‑1 (MQ=255)

                                                                                                                                                                                                                           cAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTAttct                         >  1:1498254/1‑238 (MQ=255)

                                                                                                                                                                                                                              tatGATCTTCTATCCATTGATTCCCCCCTTGGT‑CGTGAAAA‑CCCCCGCCGCCAGCGCCTTTTTCTGCTGGCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCAt                     <  1:1291300/239‑1 (MQ=255)

                                                                                                                                                                                                                               atGACCGTCTATCCGTTGTTCCGCCCCCTGGT‑GGGGATAA‑TCCCCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑‑TTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACg                                                                                <  2:438885/178‑1 (MQ=255)

                                                                                                                                                                                                                                   tcgtttacccgttGATCCGCCCCTGGGG‑CTTAATAA‑CCCCCGCCGCCAGCGCTTTTTTCTTCTGCCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAt                 <  2:965327/226‑1 (MQ=255)

                                                                                                                                                                                                                                    cGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAttt               >  2:42493/1‑239 (MQ=255)

                                                                                                                                                                                                                                    cGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAttt               >  1:605212/1‑239 (MQ=255)

                                                                                                                                                                                                                                     ttCTATCCGGTTATTCGCCCCCGGGT‑CGTGATAA‑TCCCCGCCGCAAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTg              <  1:32932/238‑1 (MQ=255)

                                                                                                                                                                                                                                     gctttcccAGTGTTCCCCCCCCTGGG‑CGGGATAA‑TCACCGCCGCCGGCGCTTTTTTCTGCTGTCGAACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTg              <  2:862841/233‑1 (MQ=255)

                                                                                                                                                                                                                                     gTCTACCCAGTGATCCGCCCCCGGGT‑CGTGATAA‑TCACCGCCCCAGGCGCCTTTTTCTGCTGTCGAACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTg              <  1:762260/239‑1 (MQ=255)

                                                                                                                                                                                                                                         accctgtgtTTCGCCCCCGGGC‑CGTGATAC‑TCCCCGCCGCCAGCGCCTTTTTCTGCGGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAg          <  2:656729/234‑1 (MQ=255)

                                                                                                                                                                                                                                         aTCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAg          <  1:42493/239‑1 (MQ=255)

                                                                                                                                                                                                                                         aTCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑‑TTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATcc                                                                      >  1:438885/1‑178 (MQ=255)

                                                                                                                                                                                                                                          tCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGc                                            <  1:852431/204‑1 (MQ=255)

                                                                                                                                                                                                                                          tCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGc                                            >  2:852431/1‑204 (MQ=255)

                                                                                                                                                                                                                                               tGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCa                                  <  1:1210087/209‑1 (MQ=255)

                                                                                                                                                                                                                                               tGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCa                                  >  2:1210087/1‑209 (MQ=255)

                                                                                                                                                                                                                                               tGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTa                             >  1:282927/1‑214 (MQ=255)

                                                                                                                                                                                                                                                 aTTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAcc  >  1:239467/1‑239 (MQ=255)

TCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTT‑CGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAA‑TGACTGGCG‑TGACTG‑CTCGTAATCGCGGTATTTACT‑CATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGT‑CGTGATAA‑TCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAA‑TTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTACC  >  NC_000913/2361075‑2361549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`