Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,826,367 G→A 14.1% V180I (GTT→ATT)  srlA → glucitol/sorbitol‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,826,3670GA14.1% 298.7 / 16.0 130V180I (GTT→ATT) srlAglucitol/sorbitol‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base G (64/46);  new base A (17/2);  total (81/49)
Fisher's exact test for biased strand distribution p-value = 9.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

GCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGAC  >  NC_000913/2826133‑2826599
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             
gcggcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTc                                                                                                                                                                                                                                            >  1:152746/1‑238 (MQ=255)
 cggcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTTTCTGCTGGTTGGTCTGGGCCCCAATTTCTTCCGCGGCTGGGGGA‑CCGGTCT‑TACCCCCGCCCTTTTTT‑GGGAAAAAATGGGGCTT‑CAAATTT‑AACAAAAA‑gataa                                                                                                                                                                                                                                           >  2:180394/1‑234 (MQ=255)
   gcCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGGCACCAATTTCTTCCGCGGCCGGGGGA‑CCGATCT‑GACCACCGCCATTTTTT‑GGAAAAAAATGGGCATT‑CAACCTG‑AACAAGAA‑GGTCa                                                                                                                                                                                                                                           >  1:566735/1‑236 (MQ=255)
        ttATAGCTGCCACTGAAGAAAGGCCCTCTCCCCCCATATCAACCCGGGCGAACTGTTTTTTTATCTGGGCA‑TGGCCGGCGGTCTGACAACGC‑TGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTCCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑c                                                                                                                                                                                                                                    <  2:4431/237‑1 (MQ=255)
         tgaggcgccccttaaataaatgCCTTCTCCCCCCATATCACCCCGGGCGAATTTTTTTTTTAT‑TTGGCATTTGCCGGGGGTCTGAC‑ACGCTTGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑gg                                                                                                                                                                                                                                <  2:559973/225‑1 (MQ=255)
           taGCTGCCACTCAATGAATGGCTTCTCCCCCCATATCACCCCTGGCAAATTTTTTGTTTATCTTGGCA‑TGGCCAGCGGTCTAACAACGC‑TAACCCTGCCACTTGGCCCACGGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAg                                                                                                                                                                                                                              <  2:1521796/239‑1 (MQ=255)
           taGCTCCCACTAAATGATGGGCCTCTTCCCCCAAACCACCCCGGGCGAACTTTTGTTTTATCTTGGCA‑TGGCCAGCGGTTGAACACCGC‑TGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTGGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAg                                                                                                                                                                                                                              <  2:129135/239‑1 (MQ=255)
           cgggggccacaaatgaaagcgcgtctcCCCCCATATCACACCTGCCGAAATCTGTGTTTACTTGGGTA‑TCCCCGGAGGTGTAAAAGCGC‑TAACCCCGCCCCTTGGCCTCCTGGCGGTGAGTTACCTGCTGGTTGCTTTGGTCACAAGTTTCTGCCGCGGCTGGGTGA‑CGGATCA‑AACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAg                                                                                                                                                                                                                              <  2:1208410/216‑1 (MQ=255)
              cTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTGTCTTCCGGTTGGTCTGGGCACCAATTTTTTCCGCGGCTGGGGGA‑ACGAACT‑TACCACCGCCCTTTTTT‑AGGAAAAGATGGGCATT‑TCACTTG‑AACAAAAA‑ATTCTCCTGG‑CC‑GGAG‑Ca                                                                                                                                                                                                                           >  2:1445262/1‑238 (MQ=255)
              cTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAAATTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGGATT‑CAACTTG‑AACAAAAA‑ATTCACCTGG‑CA‑GGAG‑Caa                                                                                                                                                                                                                          >  1:322367/1‑239 (MQ=255)
                gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTTGTCTGGTCACCAATTTCTTCCTCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTT‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑ATTCACCTGG‑CA‑GGAG‑CAAca                                                                                                                                                                                                                        >  1:1306574/1‑239 (MQ=255)
                gCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCCATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑TACCACCGCCCTTTTTG‑AGAAAAAGATGGGCCTT‑CAACTTG‑AACAAAAA‑AGTCACCTGG‑CA‑GGAG‑Ccaca                                                                                                                                                                                                                        >  2:145160/1‑239 (MQ=255)
                 ccACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGA‑CCGATCT‑GACCACCGCCCTTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACCAAAA‑GTTCACCTGG‑CA‑GGAG‑CAAcat                                                                                                                                                                                                                       >  1:1517676/1‑239 (MQ=255)
                 ccACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGA‑CCGATCT‑GACCACCGCCATTTTTT‑GGAAAAAGAAGGGGATT‑CAACTTGAAACAAAAA‑GTCCA‑CTTG‑CA‑GGAggcaac                                                                                                                                                                                                                         >  2:226099/1‑233 (MQ=255)
                  cACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAAca                                                                                                                                                                                                                        >  1:4431/1‑237 (MQ=255)
                    cTCATTGAATGGCTCCTTCCCCAATACCACCCCGGGCGATCTGTTTGTTTTCCTTGGCA‑TTGCCGGCGGTCTGACACCGC‑GGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTGGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAAcatcat                                                                                                                                                                                                                    <  2:511385/239‑1 (MQ=255)
                    agaaatgaatgGCCTCTCCCCCCATACCACCCCGGCGGACCTGTTTGTTTATTTTGGCA‑TTGCCGGGGGTCTGACAACGC‑TGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAAcatcat                                                                                                                                                                                                                    <  2:1105826/236‑1 (MQ=255)
                        atgaatgTCCTCTTCCCCCATATCAACCCGGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACg                                                                                                                                                                                                                <  1:147066/239‑1 (MQ=255)
                         tgaatgGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGA‑CCGATCT‑GACCCCCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACg                                                                                                                                                                                                                >  1:187698/1‑238 (MQ=255)
                         tgaatgCGCTCTCCCCCCAATTCACCCCGGGCGAACTGTTTGTTTATCTGGGCA‑TTGCCAGCGGTCTGACAGCGC‑GAACCCTGCCACTTGGCCCACTGGGGGTGAGTTATCTGCTTGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGc                                                                                                                                                                                                               <  2:701487/239‑1 (MQ=255)
                                 ctctTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGA‑CCGGTCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCAAAtt                                                                                                                                                                                                          >  1:1105826/1‑236 (MQ=255)
                                 cgctcccgtcATATCATCCCTGGCGAACTTTTTGTTTCTCTGGCCA‑TTGCCAGGGTTCGCCCACCCC‑TAACCCTGCCACTTGCCCCATGGGCGGTGAGTTATCTGTTGGTGGCTCTGGTCACAAATTTTTCCCGCGGCTGGGTGC‑CCGATCT‑GCCCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCgg                                                                                                                                                                                                       <  2:1011266/230‑1 (MQ=255)
                                   ctTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑ATTAACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCggg                                                                                                                                                                                                      >  1:118627/1‑237 (MQ=255)
                                        cccATACCACCCCGGGCGAACTTTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGaaa                                                                                                                                                                                                <  1:180394/239‑1 (MQ=255)
                                          cacatCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTTCTGGGTGGTCTGGGCACCAATTTCTTCCGCGGCTGGGGGA‑CCCATCT‑GACCACCGCCCTTTTTG‑GGAAAAAGATGGGCATT‑TAACTTT‑ACCAAAAA‑GGTCCACTGG‑CC‑GGAG‑AAACATCATGACGCATATTTCGGTCGaaaaa                                                                                                                                                                                              >  2:1314561/4‑239 (MQ=255)
                                          cATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCCATCT‑GACCACCGCCCTTTTTT‑GGAAAAAGATGGGCATT‑CACATTG‑AACAAAAA‑AGTTACCTGG‑GA‑GGGGCCAACA‑CATGACGCCTATTCGGATCGaaaaa                                                                                                                                                                                              >  1:1033260/1‑239 (MQ=255)
                                                      ggCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGGTCT‑GACCCCCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTT‑GACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGGTCGCAAAAGGAAC‑GGGTGGc                                                                                                                                                                                 >  1:302869/1‑239 (MQ=255)
                                                      ggCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCCCGGCTGGGTGA‑CCGATCT‑GACCACCGCCCTTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑ATTCACCTGG‑CA‑GGAG‑CAACATCATGACGCCTATTCGGATCGAAAAAGGAAC‑GGGGGGc                                                                                                                                                                                 >  2:1270187/1‑239 (MQ=255)
                                                      ggCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGGCACCCATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑TGACACCGCCATTTTTT‑AGGAAAAGATGGGCATT‑CAACTTT‑AACAAAAA‑GTTCACCTGG‑CC‑GGAG‑CAACATCATTACGCCTATTCGGATCGAAAAAGGAAC‑CGGTgg                                                                                                                                                                                  >  1:723307/1‑238 (MQ=255)
                                                       gCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTTCTGGTTGGTCTGGGCACCCATTTCTTCCCCGGCTGGGGGA‑CCGATCT‑GACCACCGCCCTTTTTG‑AGAAAAAAATTGGGATT‑CAACTTG‑GACAAAAA‑GTTCACCCGG‑CA‑GGTG‑CAACATCATGGCGCACATTTGGATCGAAAAAGGAAC‑cgggg                                                                                                                                                                                   >  2:692379/1‑234 (MQ=255)
                                                       gCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGAGCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGGATT‑CAACTTG‑AACAAAAA‑GTTTCCCTCG‑CA‑GGGG‑GAAAATCATGGCTCATATTCGGGTCGAAACAGGAAC‑ggggg                                                                                                                                                                                   >  2:972857/1‑234 (MQ=255)
                                                       gCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGA‑CCGATCT‑GACCACCGCCATTTTTT‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑ATTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAA‑ggggg                                                                                                                                                                                   >  1:1329994/1‑234 (MQ=255)
                                                            cTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCCCCAATTTCTTCCGCGGCTGGGGGA‑CCGATCT‑GACCACCGCCATTTTTT‑GGAAAAAGATGGGGATT‑CCACTTG‑AACAAAAA‑GTTAACCCTG‑CA‑GGAG‑CAACATACAGAAGCATATTTTGGTCGAAAAAGGAAA‑GGGGGGCTGGgg‑c                                                                                                                                                                          >  2:583024/1‑239 (MQ=255)
                                                            cTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGATATCTGCTGGTTGGTTTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACAACCGCCATTTTTG‑AGACAAAGATGGGCATT‑CAACTTC‑AACAAAAA‑GTTCACATGG‑CA‑GGAG‑CCACAACATGAAGCATATTTGGATCGAAAAAGGAAC‑GGGCGGCTGGgg‑c                                                                                                                                                                          >  1:637271/1‑239 (MQ=255)
                                                              gtttgtttTTCTTGGCA‑TTGCCAGCGGTCTGACACCGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGgg‑cgg                                                                                                                                                                        <  1:1264556/239‑1 (MQ=255)
                                                                         ttGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTTGCCCACTGGCGGGGAGTTATACTCCGGTTGGGGTGGGTACCAATTTCATCTCGGGGTGGGGGA‑CCGCTCTACACCA‑CGGCATTTTTT‑CGAACAAAAAGGGGATT‑TAACCTT‑AAAAAAAA‑GTTCCCCTCG‑CC‑GGGG‑GAACGACATGAAGCCTATTCGGGTGGTAAAAAGAAC‑GGGGGGCTGGGGAGGGCCCGCtggg                                                                                                                                                               >  2:1001942/1‑235 (MQ=255)
                                                                          tGGCG‑TTGCCAGCGGTCGAACAACGC‑TGAACCTGCCACTTGGCCCACGGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCt                                                                                                                                                            <  1:256532/239‑1 (MQ=255)
                                                                          tGGCA‑TTGCCAGCGGTCTGACAACGC‑TAACCCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGC‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCt                                                                                                                                                            <  1:1273990/239‑1 (MQ=255)
                                                                            gCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGGTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGGTCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCAACTGG‑CA‑GGAG‑CAACAACATGACGCATATTCGGATCGAAAAAGGGAC‑GGGTGGCTGGGG‑CGGGCCGCTTTAGCTGa                                                                                                                                                          >  1:711770/1‑239 (MQ=255)
                                                                                  gCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTTGTCTGGTCACCAATTTCTTCCGCGGCTGGGGGA‑CCGATCT‑GACCACCGCCATTTTTT‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GATCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTTGGATTGAAAAAAGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTTAGGTGAAAggc                                                                                                                                                     >  2:359738/1‑237 (MQ=255)
                                                                                  gCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GGCCACCGCCATTTTTG‑AGAAAAAGATGGGCCTT‑CAACTTG‑AAAAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGGGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGc                                                                                                                                                      >  1:516296/1‑238 (MQ=255)
                                                                                  gCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGGTATCTGCTGGTTGGGCTGGTCACCACTTTCTTCCGCGGCTGGGGGA‑ACGATAT‑GGCCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CCAATTG‑AACAAAAA‑AGTCACCTGG‑CA‑GGGG‑GAACATCATTACGCATATTTCGCACGGAAAAGGAAC‑GGGGGGGTGGGG‑CGGCCCGCCTTGGCTGCAAGcc                                                                                                                                                     >  2:789996/1‑239 (MQ=255)
                                                                                         gttggACACCGT‑TGACCCGGCCCCTGGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCGGGTCACAAATTTCTTCCGCGGCTGGGTGA‑CCGATTT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCg                                                                                                                                               <  1:369202/234‑1 (MQ=255)
                                                                                                  cGC‑TGACCCTGCCCCTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                                                                                     <  1:702651/239‑1 (MQ=255)
                                                                                                   ggctaaccgtcccACTGGGCCCGCGGGCGTTGCGTTATCTGCTGGATGGTCTGGTCACCAATTGCTCCCCCGGCTGGGCGA‑CCGATGT‑GACCACCGCCATTTTTG‑AGAAAAAAATGGGCATT‑CAACTTG‑AACAAAAA‑GTGCACCTGG‑AA‑GGAG‑CAACATCATGACGCATATTCGGAGCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                                                                                     <  2:77176/228‑1 (MQ=255)
                                                                                                    c‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAAAATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCCCTTGAGCTGAAAACCACGCCGGGAAAAAAAAta                                                                                                                                   >  1:1238865/1‑238 (MQ=255)
                                                                                                    c‑TGAACCTGCCACTTGGCCCACTGGCGGGGAGGTTTCTGCCGGTTTGTCTGGTCACCAATTTCTTCCCCGGCTGGGTGA‑ACGATCT‑GACCACCGCCATTTTTG‑GGAAAAAAGAGGGCATT‑TAAACTG‑AACAAAAA‑ATTCACCTGG‑CA‑GGAG‑CAAAATCATGACCCCTATTACGGTCGGAAAAAGAAA‑CGGTGGCTGGGG‑CGGGCAGCTTGAGCCGGAAGGCCCGCCGGGGAAAAAAATc                                                                                                                                   >  2:104455/1‑239 (MQ=255)
                                                                                                     ggaacccTCCCCCTTGCCCCCCTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCGGGGTGA‑CCGTTTT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATc                                                                                                                                   <  1:1445262/234‑1 (MQ=255)
                                                                                                             gCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGGGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCCCGCCGGGAAAAAAAATCGTCtata                                                                                                                            >  1:1145291/1‑238 (MQ=255)
                                                                                                              ccACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTTTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTT‑AGAAAAATATGGGCATT‑CAACTTG‑AACAAAAA‑ATTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAAGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTTAGCTTAAAGCCACGCCGGGAAAAAAAATCGTCTatat                                                                                                                           >  1:1329421/1‑238 (MQ=255)
                                                                                                                     gCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGGAAAAAAATCGTCTATATCAccgccg                                                                                                                   >  1:833761/1‑239 (MQ=255)
                                                                                                                         aCTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGGAAAAAAAACGGCTATATCACCGCCggga                                                                                                                >  1:148697/1‑236 (MQ=255)
                                                                                                                                gTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCCTTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGGGGCTGGGG‑CGGCCCGCTTGAGCTGGAAGCCACGCCGGGGAAAAAAATCGTCTATATACACGCCGGTACCCGGcc                                                                                                         >  2:381777/1‑238 (MQ=255)
                                                                                                                                   gggtgtcgggggggtggtctggtcCCCAATTTTCTCCCGGGGGGGGTGACCCTATCG‑GCCCCCCGCCATTTTGG‑AAAAAAAGAGGGGAATC‑CAACTTA‑AAAAAAAA‑GTCCACCGGG‑CG‑GGAG‑CAAAATCAGGCCGCATATCCGGACAAAAAAAGGAAC‑GGGGGGCGGGGG‑CGGCCCGCTTGAGCTGAAAGCCCCGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTg                                                                                                       <  2:736709/224‑1 (MQ=255)
                                                                                                                                       aTCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCAACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGAttg                                                                                                 >  2:66478/1‑239 (MQ=255)
                                                                                                                                          tgctgGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGAttgttg                                                                                              >  1:1475105/1‑239 (MQ=255)
                                                                                                                                           gctgGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGa                                                                                             >  2:1117476/1‑239 (MQ=255)
                                                                                                                                            ctgGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGAc                                                                                            >  1:1020944/1‑239 (MQ=255)
                                                                                                                                             tggttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACa                                                                                           >  1:342587/1‑239 (MQ=255)
                                                                                                                                             tggttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTAACCGGCCTGCGATTGTTGACa                                                                                           >  1:1189489/1‑239 (MQ=255)
                                                                                                                                             tggttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CAGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACa                                                                                           >  1:1092617/1‑239 (MQ=255)
                                                                                                                                             ggtgtttcttgtccACCAATTTTTCCCACGGCTGGGGGC‑CCCATCT‑CACCCCCCGCTTTTTTGAAAAAAAAGAT‑GGTAAT‑CAAGTTT‑AAAAAAAATGTTCCCCTGG‑CA‑GGAG‑AAACATAATGCCGAAACTGGGGATAGAAAAAGAAAC‑GGGGGGCTGGGG‑GGCCCCGTTTGAGCAAAAACCCCCGCGGGAAAAAAAAATCGTTTTTTTCCCCGCCGGTACCCGGCCTGCGATTGTTGa                                                                                             <  2:1290444/225‑1 (MQ=255)
                                                                                                                                               gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGaa                                                                                                                                                                                                 >  2:312030/1‑137 (MQ=255)
                                                                                                                                               gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGaa                                                                                                                                                                                                 <  1:312030/137‑1 (MQ=255)
                                                                                                                                               gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                          >  1:1027018/1‑238 (MQ=255)
                                                                                                                                               gttggtCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACaa                                                                                          >  1:1326878/1‑238 (MQ=255)
                                                                                                                                                  ggtctggtcACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGCCAAACTg                                                                                      >  2:124169/1‑239 (MQ=255)
                                                                                                                                                         tcACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcaca                                                                                 >  1:950600/1‑237 (MQ=255)
                                                                                                                                                         tcACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGcaca                                                                                 >  1:760953/1‑237 (MQ=255)
                                                                                                                                                           aCCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCtt                                                                             >  1:274755/1‑239 (MQ=255)
                                                                                                                                                                  tcttcCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActg                                                                         >  1:1113855/1‑236 (MQ=255)
                                                                                                                                                                  tcttcCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTActg                                                                         >  1:344113/1‑236 (MQ=255)
                                                                                                                                                                     ccgcggggggggaaa‑CCGATGT‑CCCCACCGCAATTTTAA‑AAAAAAAGATGGCTATT‑AAATATG‑AAAAAAAA‑GTCCCCCGGG‑CA‑GGAC‑AAACTGCATGACGTATATGGGGACAGAAAAGGAAAC‑GGGGGGGGGGGG‑CGTCCCGGTTGAGGTAAAACCCCCGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACtggctggc                                                                   <  2:1306574/225‑1 (MQ=255)
                                                                                                                                                                      cccgggggggagaccccGATCT‑CCCCCCCCCCTTTTTTA‑AAAAAAAGATGGGAATC‑CAACTTA‑AAAAAAAA‑TTTCCCCGGG‑GC‑GGAC‑AACCACAATGACGTATTTACGGAAAAAAAAAGGAAG‑GGGTGGGTGGGG‑CGGCCCGTTTGAGCTAAAAGCCACGCCGGAAAAAAAAATCGTCAATATCTCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACtggctggc                                                                   <  2:344113/224‑1 (MQ=255)
                                                                                                                                                                       cgcgGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAg                                                                 >  1:800957/1‑239 (MQ=255)
                                                                                                                                                                           gCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAGGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTa                                                             >  2:909902/1‑239 (MQ=255)
                                                                                                                                                                           gCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTa                                                             >  1:69369/1‑239 (MQ=255)
                                                                                                                                                                                gTGA‑CCTATCG‑CCCCACCGCCATTTTTG‑AGAAAAAGAGGGGCATT‑CAATTTA‑AAAAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGAAAC‑GGGGGGCGGGGG‑CGGCCCGCTTGAGCGGAAACCCACGCGGGAAAAAAAAACCGTTTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                         <  1:613027/238‑1 (MQ=255)
                                                                                                                                                                                  gtgcactagctcccccccccctttttgG‑AAAAAAAAAGGGCCATT‑AACTTTA‑AAAAAAAA‑GTCCACGGGG‑CA‑GGGG‑AAACATCAGGACGAATTTGCGGATCAAAAAAGGAAG‑GGGGGGCTGGGG‑CGGCCCGCTTGGGCGAAAAGCCACGCGGGAAAAAAAAACCGTCTATATCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                         <  2:317217/216‑1 (MQ=255)
                                                                                                                                                                                  gtccccatatgacccaccGCAATTTTGA‑AGAAAAAGATGGGAATC‑AAACTTA‑ACAAAAAA‑GTTCACCGGG‑CA‑GGAG‑CACCATCATGACGAATATTCGGATGAAAAAAGGAAG‑GGGTGGGGGGGG‑CGGCCCGCTTGGGCTGAAAGCCCCGCGGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                         <  1:1405443/225‑1 (MQ=255)
                                                                                                                                                                                  ggcaccatttcgccccccGCCTTTTTAG‑AAAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CG‑GGAG‑CAACAGCATGACGCATATTCGGAGCAAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCGGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                         <  2:148697/222‑1 (MQ=255)
                                                                                                                                                                                  ggacgtatccacccaccccccttttttG‑AAAAAAAGAT‑GGAAAT‑AAACTTA‑AAAAAAAA‑ATTCCCCCGGTGA‑GGAG‑AAACACCGAGCCGAATTTTGGGAAGAAAAAGGGAAG‑GGGTGGGTGGGG‑GGGCCGTCTGGGGCTGAAAGCCACGCGGGAAAAAAAAATCTTCTAAATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                         <  2:1538110/216‑1 (MQ=255)
                                                                                                                                                                                   ccgccgtttagccccccccattttttG‑AAAAAAAGA‑GGGCATTACAACTAG‑AAAAAAAA‑GTACCCTTGG‑CG‑GAGG‑AAACATCAGGACACATGTTGGGAGAAAAAAAGGAAC‑GGGTGGGTGGGG‑GGGCCCGCTGGGTCTGAAACCCCCGCGGGAAAAAAAAACCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGAc                                                        <  2:516296/217‑1 (MQ=255)
                                                                                                                                                                                       gATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGa                                                         >  1:1534775/1‑232 (MQ=255)
                                                                                                                                                                                        ttCT‑AACCCCCGCAATTTTTG‑AAAAAAAGAGGGTCATC‑AAACTTA‑AAAAAAAA‑GTCCCCCGGG‑CA‑GGAG‑CAACATCATGCCGCATATTGGGATAAAAAAAGGAGC‑GGGTGGCGGGGG‑CGGCCCGTTTGAGCGGAAAGCCACGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTa                                                 <  1:359738/238‑1 (MQ=255)
                                                                                                                                                                                             gACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGAGGCTGGGG‑CGGACCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCAAAGCTTACTGGCTGGCAGGCTATTGACGGATTTAaaga                                             >  1:1160797/1‑239 (MQ=255)
                                                                                                                                                                                                ccccGCCATTTTTA‑AAAAAAAGAGGGGCATT‑CAACTAG‑AAAAAAAA‑GTCCCCCTGG‑AA‑GGAG‑AAACATCAGGACGCATATTGGGATAAAAAAAGAAGG‑GGGTGGCTGGGG‑CGGCCGGCTTGAGCTGAAAGCCCCGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAgg                                          <  1:583024/237‑1 (MQ=255)
                                                                                                                                                                                                 cccGCCTTTTTGG‑AGAAAAGGAGGGGCTCA‑CCTCTAG‑AACAAAAA‑GTCCACCTGG‑AG‑GGAG‑AACCATCAGGGCGAATCTTCGGCTAAAAAAAGG‑AG‑GGGGGGCTGGGGCCGCCCCGCTGGAGCGGAAAGCCACGCGGAGAAAAAAAATCGTCTAACTCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGt                                         <  2:1212987/238‑1 (MQ=255)
                                                                                                                                                                                                   cGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                                                                                     >  1:854994/1‑145 (MQ=255)
                                                                                                                                                                                                   cGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGaaaaaaaa                                                                                                                                     <  2:854994/145‑1 (MQ=255)
                                                                                                                                                                                                   cGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGa                                       >  1:1392156/1‑239 (MQ=255)
                                                                                                                                                                                                   cGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGGGa                                       >  1:789515/1‑239 (MQ=255)
                                                                                                                                                                                                        tttttG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAAccc                                   >  1:308470/1‑238 (MQ=255)
                                                                                                                                                                                                        tttttG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAAccc                                   >  1:789945/1‑238 (MQ=255)
                                                                                                                                                                                                                               tacAACGTG‑AAAAAAAA‑GTGCCCGGGG‑CG‑GGAA‑CAACATAAGGACACATATCCGGAGAAAAAAGGGAAG‑GGGGTGGGGGGG‑CGGCCCGCTTGAGGTGAAAGCCCCGCCGGGAAAAAAAATCGTCTATACCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg             <  2:322367/237‑1 (MQ=255)
                                                                                                                                                                                                                                ttaaCATG‑AAAAAAAA‑ATTCACCCGG‑GAGGGAG‑AAACACAAGGCCGCATATCGGGACCGAAAAAGGACG‑GGGTGGCGGGGG‑CGCCCCGCTGGGGCGAAAAGCCCCGCCGGAAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTcg             <  2:69369/237‑1 (MQ=255)
                                                                                                                                                                                                                                       g‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATc     >  1:368973/1‑239 (MQ=255)
                                                                                                                                                                                                                                          aCAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCg    >  2:43528/1‑238 (MQ=255)
                                                                                                                                                                                                                                          aCAAAAA‑GGTCAGCGGG‑AG‑GGAG‑AAACACCATGACGCATATGGGGAACAAAAAGGGACG‑GGGTGGCGGGGG‑CGCCCCCCTTGAGCTGAAACCCCCGCGGGAAAAAAAAATCGTCTATACCCCCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGa   <  2:1329994/239‑1 (MQ=255)
                                                                                                                                                                                                                                           aaaaaa‑GTGCACCGGG‑GG‑GGAG‑AAGAATCATGCCGAATATGC‑GAAAAAAAAAGGAACGGGGGGGGGGGGG‑CGCCCCGTTGGAGCTAAAAGCCCCGCGGGAAAAAAAAACCGTCAAAACCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGAc  <  2:1145291/238‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             
GCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTGGCA‑TTGCCAGCGGTCTGACAACGC‑TGAACCTGCCACTTGGCCCACTGGCGGTGAGTTATCTGCTGGTTGGTCTGGTCACCAATTTCTTCCGCGGCTGGGTGA‑CCGATCT‑GACCACCGCCATTTTTG‑AGAAAAAGATGGGCATT‑CAACTTG‑AACAAAAA‑GTTCACCTGG‑CA‑GGAG‑CAACATCATGACGCATATTCGGATCGAAAAAGGAAC‑GGGTGGCTGGGG‑CGGCCCGCTTGAGCTGAAAGCCACGCCGGGAAAAAAAATCGTCTATATCACCGCCGGTACCCGGCCTGCGATTGTTGACAAACTGGCACAGCTTACTGGCTGGCAGGCTATTGACGGATTTAAAGAAGGTGAACCCGCGGAGGCGGAAATTGGTGTCGCGGTAATCGAC  >  NC_000913/2826133‑2826599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑