BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,469	C→G	19.6%	S3R (AGC→AGG)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,469	0	C	G	19.6%	190.5 / 16.0	98	S3R (AGC→AGG)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (29/45); new base G (14/5); total (45/53)
Fisher's exact test for biased strand distribution p-value = 9.64e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 101 total aligned reads displayed.

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206234‑3206707

tAAAGGCCAGCTATTCAGGATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑a                                                                                                                                                                                                                                                       <  2:915549/238‑1 (MQ=255)

tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑a                                                                                                                                                                                                                                                       <  2:226218/238‑1 (MQ=255)

       cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGtaat                                                                                                                                                                                                                                                <  2:909583/238‑1 (MQ=255)

        aGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAAACCAAAACCAAAAAAGGGG‑TTGGCATTGTGGGCG‑GAAGtaatta                                                                                                                                                                                                                                              >  1:687815/1‑237 (MQ=255)

             ttCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑a                                                                                                                                                                                                                                                       <  1:449483/225‑1 (MQ=255)

                  cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑G‑AATTTTTTCAGCGCAAAACAAAAACATGGG‑TTTCCCTGATGAGCG‑ACAGTAAAAAACAACAAGcc                                                                                                                                                                                                                                    >  1:1296097/1‑238 (MQ=255)

                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGGG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                                    <  1:65630/238‑1 (MQ=255)

                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                      <  1:1259295/236‑1 (MQ=255)

                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                                    <  1:234483/238‑1 (MQ=255)

                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                                    <  2:310669/238‑1 (MQ=255)

                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAAACAAAAAAAAAAAATGGAG‑GTTTCATGATGAGCG‑GAAGtaataaaaaaaaaagagggaa                                                                                                                                                                                                                               >  1:712410/1‑224 (MQ=255)

                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAAAAATAAAAAAAATGGAG‑TTGCCATGATGGGCG‑AAAATAATAAGCAACAAGAAATGaa                                                                                                                                                                                                                               >  1:853033/1‑239 (MQ=255)

                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑T‑GATTTTTCACGACAAATACAAAAAAAAGAG‑GTTACGTAGTGGGCG‑GAAGGAATAAGAAACATGCAGTGaa                                                                                                                                                                                                                               >  1:51345/1‑239 (MQ=255)

                       ttAATTATCCGCAGTCGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTTAAACAAAAACAAAAAAAGGAG‑TTGCCATGATGAGCG‑AAAAGAAAAAAAAACAAGCAgggga                                                                                                                                                                                                                               >  2:1218887/1‑235 (MQ=255)

                        tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                               <  1:1218887/238‑1 (MQ=255)

                        tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑G‑GATTTTTTAAGACAAAAAAAAAAAATGGAG‑GTGGCAAGGTGTGGGAAAAGTATTAAACaaaggacatgaat                                                                                                                                                                                                                               >  1:421644/1‑228 (MQ=255)

                                     ttGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                     <  1:1106259/219‑1 (MQ=255)

                                      tgtgATAAGCGCAGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGTAATATTCATTCTTATTTTCGCGTTGACTCACGAACTGTTAATTACATATTCACGGTGGCAAAATATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAgag‑a                                                                                                                                                                                                              <  2:195591/238‑1 (MQ=255)

                                        tgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATACAAAAAAAGGAG‑TTGCCATAGTTGGCG‑AAAGAAATAAACAAAAGACAATGAAT‑AAATTA‑ACAGAG‑ATTg                                                                                                                                                                                                           >  2:1499789/1‑239 (MQ=255)

                                            aaGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                      <  1:1255694/211‑1 (MQ=255)

                                                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATAAAAAAAATGGAG‑GTGCCCTGGTGAGGG‑GAAGTAATAAAAAACAAGCAGTGAAT‑AAAGTG‑TAAAAG‑ATTGTCGCT‑AAAttt                                                                                                                                                                                               >  1:1231640/1‑238 (MQ=255)

                                                    tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAAACAAAAAAAAAAAATGGAG‑TTTCCATGATGGGGG‑GAAGTAATAAGAAACAAGCAGTGAAA‑AAAATG‑ACAGAG‑ATTGTTGCT‑TACttt                                                                                                                                                                                               >  1:267806/1‑238 (MQ=255)

                                                      tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑AATTTTTCC‑GACAAAAAAAAAAACTAGAG‑GTGCCATGATTAGGG‑AGAATAATAAACAAAAAGCAGTGCAT‑AAGCTgttttttatacacaactgcagccg                                                                                                                                                                                                >  2:1255694/1‑215 (MQ=255)

                                                           cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTTAGACAAATACAAAAACCGGAG‑TCGCCATACTTGGCG‑GAAGTAATAAAAACCAAGCAGAGGAT‑AAAATG‑TAAGAC‑ATTATCCCC‑AACATT‑TCCGCCaa                                                                                                                                                                                      >  1:1450575/1‑238 (MQ=255)

                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGCTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑C‑AATTTTTTTAGACAGAAACACAAACTACAG‑GTGTCCTGATGAATG‑AAAGAAATAAGAAACAAGCAGTGAAT‑AAGTTG‑ATAGAG‑GTTATTGAC‑GACTTT‑TCCGTCAT‑GAttt                                                                                                                                                                                >  1:1495060/1‑239 (MQ=255)

                                                                aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCATACAAATACAAAAAAAGGGG‑TTTCCATTGTGAGGG‑AAAGAAAAAAAAAAAAGGAACAGAAT‑AAGATG‑ACATAG‑ATTTTCGCT‑AACTTT‑ACCCCCCT‑GGttt                                                                                                                                                                                >  2:1435656/1‑239 (MQ=255)

                                                                     tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACCAATACAAAAAATTGAG‑TTGCCATGATGAGCG‑AAAGTAATAAACAACAGGGCGGGAAT‑AAGATT‑ACAGAG‑ATTGTTGGT‑CAATTT‑TACGCCAT‑GCttttttc                                                                                                                                                                            >  1:732931/1‑234 (MQ=255)

                                                                            cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAACAAAAAATTGAG‑GTGTCATGATGAGCG‑GAAATAATAAGCAAAAAGCAGTGAAT‑AAGTTG‑AAAGGG‑ATTGTCGCT‑AAATTT‑ACCCACAT‑GATTTTTA‑CCCGAATg                                                                                                                                                                    >  1:871984/1‑238 (MQ=255)

                                                                               ttcttgggCGAAATATTCACTTTTATTTTAGAGTTGATACACGAACTGTAAATTACATATCCGCGGTGAAAAAAAATATAAAACGACATTTTAAGTGGTAGTTTGTGGGGGT‑G‑ATTTTTTCCAGACAAATAAAAAAACTGAAT‑TTGCCATGATGAGCA‑AAAGAAATAACCAACAGGCATTGAAT‑AAGTTG‑ACAGAG‑ATTGGCGCT‑ATCTTG‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCtg                                                                                                                                                                <  2:55924/233‑1 (MQ=255)

                                                                                 ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCAACAAAAAAAAAAAATGGGG‑TTGGCATTGTGAGCG‑GAAATAATAAACAACAAGGAGTGAAA‑AAAGTG‑AAAAAG‑AATGTCGCG‑AAAGTT‑AACGCCAT‑GATTTTTA‑caccgatggccgat                                                                                                                                                              >  2:1205986/1‑226 (MQ=255)

                                                                                  tctGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑ATTTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCtgatg                                                                                                                                                             <  1:734121/239‑1 (MQ=255)

                                                                                      gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGACAAAAAAAAAAAATGGGG‑TTGCCATGATGAGCG‑AAAGTAATAAACAACAAGCAGTGAAA‑AAATTG‑AACGAG‑ATTGTCGCG‑TACATT‑TCCCCCAT‑TATTTTTA‑CCCCAAAGACTCATTACgt                                                                                                                                                         >  1:59752/1‑239 (MQ=255)

                                                                                       cgcAATATTAATTTTTATTTCAGCGTTGATTCACAAACTGTAAATTACATATTCAGGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACgt                                                                                                                                                         <  2:187344/238‑1 (MQ=255)

                                                                                        gaaaaattcatttTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑ATTTTTTCAAGACAAATACAAAAACTGGAG‑TTGCTATGATGAGCG‑AAAGTAATAAGCAACAGCCAGTAATT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑ACCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                        <  1:1111782/233‑1 (MQ=255)

                                                                                        gaaaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCAAGACAAAAACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                        <  1:1499789/236‑1 (MQ=255)

                                                                                            tatTCATTTTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAACCCACATTTTTAGTGGTATTTTGTGGCGGT‑A‑AATTTTTCCAGAAAAATAAAAAAACTGGAG‑TTGCAATGATGAGCG‑AAATTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATGGTCGTT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                   <  2:1225370/239‑1 (MQ=255)

                                                                                            tatTCATTTTTATTTTCGCTTTGATTCACGAATTTTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCAAATTTTTAGTGGTGGTTTGTGGCGGT‑G‑AATTTTTCCAAAAAAATAAAAAAACGGGGG‑TTGCCATGATGAGCA‑AAAGTAATAAGCAACGGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                   <  2:1231640/239‑1 (MQ=255)

                                                                                            tatTCATTTTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGGGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑A‑AATTTTCCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCATTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                   <  1:1435656/239‑1 (MQ=255)

                                                                                            tatTCATTCTTATTTGCGCGTTGATTCACAAACTGTTAATTAAATATTCACGGGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAAAACAAAAACTGGAG‑TTGCCATGATAAGCG‑AAAGTAATAAGCAACGGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                   <  1:1285598/239‑1 (MQ=255)

                                                                                            tatTCATTCTTAATTTCTCTGTAATCCACGAATTGTTAATTACAGATTCACGGTGGCAAAAAATATAAAACCACATTTTTGTGGGTAGTTTGTGGGGGT‑G‑AATTTTTCCAAACAAATACAAAAACTGGTG‑TTGCAATGGTGAGCG‑AAAATAATAAGCACCAGGCAGTGAAT‑AAGTTG‑ACATTG‑ATTGACGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                   <  2:1495060/239‑1 (MQ=255)

                                                                                                    cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTTAAAAAAAAAAAAAAAATTGGG‑GTGGCATGATGAGGG‑GAAGTAATAAAAAAAAAGAAGAGGGA‑AAAATG‑AAAAAG‑AATGTCGCT‑AAATAT‑ACCCCCAA‑GATTTCTA‑CACGGAAGCCTGCTGACggtggggagtaaataa                                                                                                                                           >  2:463405/1‑224 (MQ=255)

                                                                                                     ttATTTTCGCGTTGATTCACGAATTGTTAATTACATATTAACGGAGGCAAAAAAAATAAAACCACATTTGTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAAGTGGAG‑TTGCCATGATGAGAA‑AAAAAAATAAGCAACAGGCAGTAAAG‑AAGTTG‑ACAGAG‑ATTGTCGCT‑ATCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTaaa                                                                                                                                         <  1:869/239‑1 (MQ=255)

                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAACAAAAAATGGAG‑GTGGCATGGTGAGCG‑AAAGTAAAAAAAAACAAGCAAGGAAT‑AAAATG‑ACAGAG‑ATTGTTGCT‑AAATTT‑ACCCCCAT‑GATTTTTA‑TCCCAAAGGCTGATGACGGGGGGGGt‑aaaataaaa                                                                                                                                        >  1:208935/1‑239 (MQ=255)

                                                                                                      tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGAAAAATACAAAAAATGGGG‑TTTCCATGATGAGCG‑AAAGAAAAAAACAAAAAGCAGAGAAA‑AAAATG‑AAAGAA‑ATTGTTGCT‑AAACTT‑TCCGCCCT‑GATTTTTA‑CCCCAAATCCTGATGAAGcggtggggaaaaaaaaa                                                                                                                                         >  1:87149/1‑228 (MQ=255)

                                                                                                       aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTTCA‑AAAAAAAAAAAAAATGGGG‑GTGGCATGGTGAGGG‑AAAGTAATAAACAACAAACAGTGGAA‑AAGATA‑ACAGAG‑GTTTGCGCT‑AACATT‑TTCGCACT‑GATTTTTA‑CTCCTAAGCCtgatgtggcggggggagtaaacaaac                                                                                                                                        >  2:111384/1‑218 (MQ=255)

                                                                                                            cgcgTTGATTCACGAACTGTTAATTACATATTCGCGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑A‑AATTTTTCCAGAAAAATACAAAAACTGGAT‑TTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTa                                                                                                                                  <  2:445209/239‑1 (MQ=255)

                                                                                                             gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATAAAAAAAATGGAG‑TTGGCATGATTAGCG‑GAAGAAATAAACAACAAGGAGAGAAT‑AAAATG‑AGAGAG‑AGTGTTGCT‑AAATTT‑ACCCCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGGGGAT‑AAAATAAAACAGCtca                                                                                                                                 >  1:567210/1‑237 (MQ=255)

                                                                                                              cgTTGATTATCAATCTGTTAATTGCTTCATAAGGGAGGAAAAAAATATAAAACCACATTTTTATTGTTAGTTGTTGGCGGT‑T‑AATTTTACTAAACAAATCAAAAAATTGGAT‑TGGCCAGGATGACAG‑AAAGAAACAGGAAAAAGGCCGTAAAT‑AAGTTG‑CCAGAG‑ATTGTCGCT‑ATTTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTaaa                                                                                                                                <  1:1181395/239‑1 (MQ=255)

                                                                                                                        cGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAG‑TTGCAATGATGAGCA‑AAAGAAATAAGCAACAGGTAGTGAAT‑AAGTTG‑AAAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                      <  1:894167/239‑1 (MQ=255)

                                                                                                                         gaccttttAATTACATTTTCACGGTGGAAAAAAATATAAACCCACATTTTTAGGGGTAGTTTGGGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAAAAAGCAACAGGCAGTGAAT‑‑AGTTGAACAGAG‑ATGGTCGCT‑ACCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGaaa                                                                                                                     <  1:31673/233‑1 (MQ=255)

                                                                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCCGACAAAAAAAAAAAATTGGG‑TTGCCATGATGGAGG‑GAAGAAATAAAAAAAAAGCAGTGAAT‑AAAGTG‑TGAGAGAATTTAGGTT‑AAATTT‑A‑CGCCAT‑GATTTCTT‑TCCCAAAGCCCCATGAAGGGGGGGGTAaaacaaaaacacaaaaagaggccggcacgca                                                                                                                  >  2:1488506/1‑219 (MQ=255)

                                                                                                                             tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAAACAAAAAAAAAAAATGGGG‑GTGTCATGATGAGGG‑GAAAAAATAAACAAAAGGAAGTGAAT‑AAGTTG‑AAAGAG‑AATGTCGCTAAAATTT‑ACC‑CCAT‑GATTTTTA‑CCCGAAAACCTGCTGACGTGGTGGAT‑AAAAAAAAAAAGCTAAAAGATGCCGacaacg                                                                                                                  >  1:425549/1‑233 (MQ=255)

                                                                                                                               ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCACACAAAAACAAAAAAAGGAG‑TTGCCCTCATGAGGG‑GAAAAAAAAAAAAAAAAGCAGCGAAA‑AAGTTG‑AAAGAG‑ATTGTGGCT‑AACTTT‑ATACCCAT‑GATTTTTT‑ACCCAATGCCTGTTGTAGGGGTGGGG‑AAACCAAAAAAGATTAAAGAAGGCGaaaattgg                                                                                                                >  2:819420/1‑233 (MQ=255)

                                                                                                                               ttAATTACATATTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGAAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                   <  1:425536/204‑1 (MQ=255)

                                                                                                                                       atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGACAAAAAAAAAAACTGGAG‑GTGTCTTGATGAGGG‑GAAGTAAAAAACAAAAGGGAGAGAAT‑AAGTTG‑ACAGAG‑ATTTTCGCT‑AACTTT‑ACCGCCCT‑GATTTCTT‑CCCGCATGCCTGATGACGTGGTGGAT‑AAACTAAAACAACTAAAGAATGCCGAAACGACGTC‑GGAgggg                                                                                                      >  2:91766/1‑239 (MQ=255)

                                                                                                                                        tatgccgggTGGCAAAAAAGAAAAAACCACATTTTAAGGTGTTGTTGGTGGCGTG‑T‑AATTTTTCCAGACAAACACAAAAACTGGAG‑TTGCCATGATGAGGT‑AAAGAAAAAAGCAACAGGCAGTTAAA‑AGTTTG‑ACAGAG‑TTTGTGGCT‑ATCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                     <  2:903177/232‑1 (MQ=255)

                                                                                                                                        tatTCGCGGTGAAAAAAAATAAAAAACCAAATTTTAAGTGGTAGTTTGTGGCGGT‑G‑AATTTTCCAAGACAAAAAAAAAAACGGGAG‑TTGCCTGGATGAGCG‑AAAGTAATAAGCAAACGGCAGTGAAT‑AATTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                     <  2:687815/239‑1 (MQ=255)

                                                                                                                                        tatTCACGGGGGCAAAAAATATAAAACAACATTTTAAGGGGTAGTTTGTGGGGGT‑G‑AATTTTCCCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTA‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                     <  1:323688/239‑1 (MQ=255)

                                                                                                                                        tatCCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGT‑A‑AATTTTCCCAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                     <  1:813275/239‑1 (MQ=255)

                                                                                                                                         atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTCAAACAAATAAAAAAAAAGGAGATTGCC‑TGATGAGAG‑AAAACAATAAAAAAAAAACAAGGAAA‑AAATTT‑AACGAG‑ATTGTGCCT‑AACTTT‑TCCACCCT‑AGTTTTTA‑CCCCAAAGCCCGATTGCCTGGGGGGT‑AAAAAAAAAAAACCAAAGGAAGACGaaaacccagcggtggggga                                                                                                     >  2:822932/1‑223 (MQ=255)

                                                                                                                                                tgggaaaaaaaaaTAAAACCACTTTTTTAGTGGTAGTTTGGGGGGGG‑G‑AATTTTTACAGACAAATACAAAAACTGGAG‑GTGCCATGATGAGGG‑AAAGTAATAAGCAACAGGCATTAAAT‑AAGTTG‑ACAGAG‑ATTTCCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                              <  2:853033/233‑1 (MQ=255)

                                                                                                                                                tgggaaaaaaaTAAAAAACCCAATTTTAAGTGTGAGTTTGTGGGTGT‑A‑AATTTTCCCAGACAAATAAAAAACCGGGAG‑TTCCCTGGAGGAGGG‑AAATTAATAAGCAAAAGACATTAAAG‑AAGTGG‑ACAGAG‑ATTGTCGCT‑ATCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                              <  2:513031/233‑1 (MQ=255)

                                                                                                                                                ggggaaaaaaaTATAAAACCACATTTTTGGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTGG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                              <  2:267806/233‑1 (MQ=255)

                                                                                                                                                gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAATGGGG‑TTGCCATGATGAGCG‑GAAGTAATAAAAAACAAGCAGAGAAT‑AAATTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑TCCGCCAT‑GATTTTTA‑CCCCAAAGCCCGATGACGGGGGGGGT‑AAAATAAAACAGCTAAAAGATGTCGCAAAGTCGCC‑GGGGGGGGAAATTa                                                                                               >  1:513765/1‑237 (MQ=255)

                                                                                                                                                gTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGT‑G‑AATTTTTCCAGACAAATACAAAAACGGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                              <  1:1389957/238‑1 (MQ=255)

                                                                                                                                                 tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAACAAAAACTGGAG‑TTTCCATGATGAGCG‑AAAGTAAAAAACAACAGGCAAAGAATAAAGTTG‑‑CAGAG‑ATTGTCGCT‑AAATTT‑ACCGCCAT‑GATTTTTT‑CCCCAATGCCCGATGAAGTTGGGGGT‑AAAATAAAAAAACTAAAAGGTGCCGAAAACGCGTC‑GGTGGGGGAAAATATCt                                                                                            >  2:224749/1‑239 (MQ=255)

                                                                                                                                                 tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAAACAAAAAAAAAAAATGGAG‑GTGTCATGATGAGCG‑GAAATAATAAACAAAAAGCAAGGAAT‑AAAATG‑ACAGAG‑ATTGTTGCT‑AACTTT‑ACCGCCAT‑GATTTTTACCCCCAATG‑CTTATGGCGGGGGGGGT‑AAAACAAAACAGCTAAAAGGAGCCGAAAAGGCCTCGGAGGGGGAAAAtatat                                                                                             >  2:628479/1‑235 (MQ=255)

                                                                                                                                                  agaaaaaaaTATAAACCCACATTTTTAGTGGTAGTTTGGGGCGGT‑A‑AATTTTTCAAAACAAAAACAAAAACTGGAG‑TTGCCATGATGAGGA‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGTT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTa                                                                                           <  2:348892/236‑1 (MQ=255)

                                                                                                                                                   gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAAACAAATAAAAAAAATGGAG‑TTGGCATGATTAGCG‑AAAGGAATAAAAAAAAGGCAGGGAAT‑AAATTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCCT‑GATTTTTA‑CCCCAAAACCTGATGAAGTGGGGGGT‑AAAATAAAACAACTAAAAGAAGCCCAAAACTCGTC‑GGTGGGGGAAAATATTTa                                                                                           >  1:405616/1‑238 (MQ=255)

                                                                                                                                                    cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAATGGAG‑GTGGCATGATGAGCG‑GAAGTAAAAAACAAAAAGCAGAGAAT‑AAAATG‑ACAAAG‑ATTGTCGGC‑AACTTT‑TACACCCT‑GATTTTTT‑CCCCGATGGCTCATGAAGTGGGGGGT‑AAAATAAAAAAACGAAAAGAGACCGAAAAGTCGCC‑GGTGGGGGAAATTATATAcc                                                                                         >  2:1389957/1‑239 (MQ=255)

                                                                                                                                                       aaaaTATAAACCCAAATTTTAAGTGGTAGTTTGGGGCGGT‑A‑AATTTTCCCAAAAAAATAAAAAAACTGGAT‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATa                                                                                      <  1:303345/239‑1 (MQ=255)

                                                                                                                                                         aaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                    <  1:856817/239‑1 (MQ=255)

                                                                                                                                                         aaTAAAAAACCACAGTTATAGTGGTAGTTTGTGGCGGT‑A‑AATTTGCCAAGAAAAAAACAAAAACGGGAG‑TTGCAATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCTCA‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                    <  2:732931/239‑1 (MQ=255)

                                                                                                                                                            ataAACCCAAATTTTGAGGGGTATTGTGTGGGGGT‑G‑AATTTTGCCAAAAAAATACAAAAACTGGAT‑TTGCTATAAGGAGCG‑AAAGTAAAAAACAACAGGCAGTGAAA‑AAGTTG‑ACAGAG‑ATTGTCGAT‑ACCTTA‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATg                                                                                 <  2:1296097/239‑1 (MQ=255)

                                                                                                                                                             taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑AATTTTTCC‑GACAAAAAAAAAAAATGGAG‑TTGGCATGTTGGGGG‑GAAAAAATAAACAAAAAGCAGTGGAT‑AAAATT‑AAAGAA‑AATGTTGCT‑AAATTTAACCCCCAT‑‑ATTTTTA‑CCCCAATGCCCGATGACGGGGGGGGG‑AAAAAAAAAAAGCTAAAAGATGGCGGAACATCGGC‑GGTGGGGGAAAATATATAACATAcaaagt                                                                                >  2:837292/1‑234 (MQ=255)

                                                                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGACAAAAACAAAAAATGGGG‑TTGCCATGATGAGGG‑AAAGAAATAAGCAACAGGCAGTGAAA‑AAATTG‑AAAGAG‑ATTGTCTCT‑AACTTT‑ACCGCCCT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGGT‑AAAATAAAAAAGCTAAAGGATGCCGAAAAGTCGTC‑GGTGGGGAAAATTATATAACATCAGATTTTCGacaac                                                                        >  1:1188013/1‑239 (MQ=255)

                                                                                                                                                                     caTTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTAAAAAAAAAAAAAAAAATGGAG‑TCGCCATTATGAGGG‑GAAGGAATAAAAAACAAGCAGGGAAA‑AAAATT‑AAAAAG‑GTTGTCGCT‑AACTTT‑TTCGCCCTGGTTTTTTT‑CCCGAA‑GCCTGATGGAGTGGGGGGT‑AAACTAAAAAAACTAAAAGAAGCCGAAACAGCGTT‑GGTGAGGGAAAATATTTACCATaacgtgtttgacgac                                                                        >  2:257975/1‑225 (MQ=255)

                                                                                                                                                                              tgggattttGTGGGGGT‑G‑AATTTTTCCAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                <  2:712410/232‑1 (MQ=255)

                                                                                                                                                                                  ggttggggggggT‑A‑AATTTTACAAAAAAAATAAAAAACCTGGAG‑TTGCAATGATGAGCG‑AAATAAATAAGCACAAGGCAGTAAAT‑AAGTTG‑ACAGAG‑TTTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  2:425549/229‑1 (MQ=255)

                                                                                                                                                                                  aGTGTGTGGTGGT‑G‑AATTTTTCCAAAAAAATACAAAAACTGGAG‑TTGCTATGATGAGCG‑AAAGTAATAAGCAACGGGAAGGTAAT‑AAGTTG‑AAATAG‑ATTGTCGTT‑CACTTT‑TCCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                           <  1:779026/239‑1 (MQ=255)

                                                                                                                                                                                         gtgcatgtaaaTTTTACCAAACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTATAAAGCAACAGGCAGTAAAT‑AAGTTG‑AGAAAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                      <  1:382967/230‑1 (MQ=255)

                                                                                                                                                                                                      ttccaaGAAAAATACAAAAATTGAAG‑TTGCAATGAGGGGAG‑AAAGAAAAAACCAACAGGCAGTGAAT‑AATTTG‑ACAGAG‑ATTGTCCAT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  1:579119/234‑1 (MQ=255)

                                                                                                                                                                                                            gACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                   >  2:679607/1‑239 (MQ=255)

                                                                                                                                                                                                             aaaaaaaaaaaaaCTGGAG‑TTGCAATGATGAGCA‑AAAGTAAAAAGCAAAAGGAAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc                                           <  2:1222728/222‑1 (MQ=255)

                                                                                                                                                                                                                     aaaaaaTGGAG‑TTGCCATGATGAGCT‑AAAGTAATAAGCAACAGGAGGTGAAT‑AAGTTG‑ACAGAT‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                          <  2:82481/239‑1 (MQ=255)

                                                                                                                                                                                                                       aaaCTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                 >  1:1222728/1‑230 (MQ=255)

                                                                                                                                                                                                                       aaaCTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                        >  1:951176/1‑239 (MQ=255)

                                                                                                                                                                                                                       aaaCTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                        >  1:819506/1‑239 (MQ=255)

                                                                                                                                                                                                                        aaCTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGAAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGAT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:1465131/239‑1 (MQ=255)

                                                                                                                                                                                                                        aaCTGGAG‑TTGCCAGGAGGAGCG‑AAAGAAAAAAGCAACAGGCAGTGAAT‑AAGTTG‑AAAGAG‑ATGGCCGCT‑AACTTA‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:454149/239‑1 (MQ=255)

                                                                                                                                                                                                                        aaCGGGAG‑TTGCAATGAGAAGGA‑AAAGTAATAAGCAACAGGCAGGGAAT‑AAGTTA‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:513765/239‑1 (MQ=255)

                                                                                                                                                                                                                         aCTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCGTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                      >  1:347329/1‑239 (MQ=255)

                                                                                                                                                                                                                         aCTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                      >  1:1512087/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt              >  2:1114524/1‑238 (MQ=255)

                                                                                                                                                                                                                                   gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt              >  1:1294000/1‑238 (MQ=255)

                                                                                                                                                                                                                                   gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGTT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt             <  1:463405/239‑1 (MQ=255)

                                                                                                                                                                                                                                     cATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttct           <  1:837292/239‑1 (MQ=255)

                                                                                                                                                                                                                                              cG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  1:961929/1‑239 (MQ=255)

                                                                                                                                                                                                                                              cG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  1:175359/1‑239 (MQ=255)

                                                                                                                                                                                                                                              cG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  1:574027/1‑239 (MQ=255)

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206234‑3206707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`