Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,504 C→A 20.3% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,5040CA20.3% 252.5 / 15.9 111T15K (ACA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (40/44);  new base A (13/10);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206272‑3206740
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
tgtgATAAGCGCAGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGTAATATTCATTCTTATTTTCGCGTTGACTCACGAACTGTTAATTACATATTCACGGTGGCAAAATATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAgag‑a                                                                                                                                                                                                                                               <  2:195591/238‑1 (MQ=255)
  tgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATACAAAAAAAGGAG‑TTGCCATAGTTGGCGAAAGAAATAAACAAAAGACAATGAAT‑AAATTA‑ACAGAG‑ATTg                                                                                                                                                                                                                                            >  2:1499789/1‑239 (MQ=255)
              tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATAAAAAAAATGGAG‑GTGCCCTGGTGAGGGGAAGTAATAAAAAACAAGCAGTGAAT‑AAAGTG‑TAAAAG‑ATTGTCGCT‑AAAttt                                                                                                                                                                                                                                >  1:1231640/1‑238 (MQ=255)
              tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAAACAAAAAAAAAAAATGGAG‑TTTCCATGATGGGGGGAAGTAATAAGAAACAAGCAGTGAAA‑AAAATG‑ACAGAG‑ATTGTTGCT‑TACttt                                                                                                                                                                                                                                >  1:267806/1‑238 (MQ=255)
                     cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTTAGACAAATACAAAAACCGGAG‑TCGCCATACTTGGCGGAAGTAATAAAAACCAAGCAGAGGAT‑AAAATG‑TAAGAC‑ATTATCCCC‑AACATT‑TCCGCCaa                                                                                                                                                                                                                       >  1:1450575/1‑238 (MQ=255)
                          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGCTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑C‑AATTTTTTTAGACAGAAACACAAACTACAG‑GTGTCCTGATGAATGAAAGAAATAAGAAACAAGCAGTGAAT‑AAGTTG‑ATAGAG‑GTTATTGAC‑GACTTT‑TCCGTCAT‑GAttt                                                                                                                                                                                                                 >  1:1495060/1‑239 (MQ=255)
                          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCATACAAATACAAAAAAAGGGG‑TTTCCATTGTGAGGGAAAGAAAAAAAAAAAAGGAACAGAAT‑AAGATG‑ACATAG‑ATTTTCGCT‑AACTTT‑ACCCCCCT‑GGttt                                                                                                                                                                                                                 >  2:1435656/1‑239 (MQ=255)
                               tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACCAATACAAAAAATTGAG‑TTGCCATGATGAGCGAAAGTAATAAACAACAGGGCGGGAAT‑AAGATT‑ACAGAG‑ATTGTTGGT‑CAATTT‑TACGCCAT‑GCttttttc                                                                                                                                                                                                             >  1:732931/1‑234 (MQ=255)
                                      cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAACAAAAAATTGAG‑GTGTCATGATGAGCGGAAATAATAAGCAAAAAGCAGTGAAT‑AAGTTG‑AAAGGG‑ATTGTCGCT‑AAATTT‑ACCCACAT‑GATTTTTA‑CCCGAATg                                                                                                                                                                                                     >  1:871984/1‑238 (MQ=255)
                                         ttcttgggCGAAATATTCACTTTTATTTTAGAGTTGATACACGAACTGTAAATTACATATCCGCGGTGAAAAAAAATATAAAACGACATTTTAAGTGGTAGTTTGTGGGGGT‑G‑ATTTTTTCCAGACAAATAAAAAAACTGAAT‑TTGCCATGATGAGCAAAAGAAATAACCAACAGGCATTGAAT‑AAGTTG‑ACAGAG‑ATTGGCGCT‑ATCTTG‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCtg                                                                                                                                                                                                 <  2:55924/233‑1 (MQ=255)
                                           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCAACAAAAAAAAAAAATGGGG‑TTGGCATTGTGAGCGGAAATAATAAACAACAAGGAGTGAAA‑AAAGTG‑AAAAAG‑AATGTCGCG‑AAAGTT‑AACGCCAT‑GATTTTTA‑caccgatggccgat                                                                                                                                                                                               >  2:1205986/1‑226 (MQ=255)
                                            tctGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑ATTTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCtgatg                                                                                                                                                                                              <  1:734121/239‑1 (MQ=255)
                                                gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGACAAAAAAAAAAAATGGGG‑TTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAAA‑AAATTG‑AACGAG‑ATTGTCGCG‑TACATT‑TCCCCCAT‑TATTTTTA‑CCCCAAAGACTCATTACgt                                                                                                                                                                                          >  1:59752/1‑239 (MQ=255)
                                                  gaaaaattcatttTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑ATTTTTTCAAGACAAATACAAAAACTGGAG‑TTGCTATGATGAGCGAAAGTAATAAGCAACAGCCAGTAATT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑ACCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                                                         <  1:1111782/233‑1 (MQ=255)
                                                  gaaaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCAAGACAAAAACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                                                         <  1:1499789/236‑1 (MQ=255)
                                                      tatTCATTTTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAACCCACATTTTTAGTGGTATTTTGTGGCGGT‑A‑AATTTTTCCAGAAAAATAAAAAAACTGGAG‑TTGCAATGATGAGCGAAATTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATGGTCGTT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                    <  2:1225370/239‑1 (MQ=255)
                                                      tatTCATTTTTATTTTCGCTTTGATTCACGAATTTTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCAAATTTTTAGTGGTGGTTTGTGGCGGT‑G‑AATTTTTCCAAAAAAATAAAAAAACGGGGG‑TTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                    <  2:1231640/239‑1 (MQ=255)
                                                      tatTCATTTTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGGGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑A‑AATTTTCCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCATTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                    <  1:1435656/239‑1 (MQ=255)
                                                      tatTCATTCTTAATTTCTCTGTAATCCACGAATTGTTAATTACAGATTCACGGTGGCAAAAAATATAAAACCACATTTTTGTGGGTAGTTTGTGGGGGT‑G‑AATTTTTCCAAACAAATACAAAAACTGGTG‑TTGCAATGGTGAGCGAAAATAATAAGCACCAGGCAGTGAAT‑AAGTTG‑ACATTG‑ATTGACGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                    <  2:1495060/239‑1 (MQ=255)
                                                              cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTTAAAAAAAAAAAAAAAATTGGG‑GTGGCATGATGAGGGGAAGTAATAAAAAAAAAGAAGAGGGA‑AAAATG‑AAAAAG‑AATGTCGCT‑AAATAT‑ACCCCCAA‑GATTTCTA‑CACGGAAGCCTGCTGACggtggggagtaaataa                                                                                                                                                                            >  2:463405/1‑224 (MQ=255)
                                                               ttATTTTCGCGTTGATTCACGAATTGTTAATTACATATTAACGGAGGCAAAAAAAATAAAACCACATTTGTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAAGTGGAG‑TTGCCATGATGAGAAAAAAAAATAAGCAACAGGCAGTAAAG‑AAGTTG‑ACAGAG‑ATTGTCGCT‑ATCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTaaa                                                                                                                                                                          <  1:869/239‑1 (MQ=255)
                                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAACAAAAAATGGAG‑GTGGCATGGTGAGCGAAAGTAAAAAAAAACAAGCAAGGAAT‑AAAATG‑ACAGAG‑ATTGTTGCT‑AAATTT‑ACCCCCAT‑GATTTTTA‑TCCCAAAGGCTGATGACGGGGGGGGt‑aaaataaaa                                                                                                                                                                         >  1:208935/1‑239 (MQ=255)
                                                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTTCA‑AAAAAAAAAAAAAATGGGG‑GTGGCATGGTGAGGGAAAGTAATAAACAACAAACAGTGGAA‑AAGATA‑ACAGAG‑GTTTGCGCT‑AACATT‑TTCGCACT‑GATTTTTA‑CTCCTAAGCCtgatgtggcggggggagtaaacaaac                                                                                                                                                                         >  2:111384/1‑218 (MQ=255)
                                                                      cgcgTTGATTCACGAACTGTTAATTACATATTCGCGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑A‑AATTTTTCCAGAAAAATACAAAAACTGGAT‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTa                                                                                                                                                                   <  2:445209/239‑1 (MQ=255)
                                                                       gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAATAAAAAAAATGGAG‑TTGGCATGATTAGCGGAAGAAATAAACAACAAGGAGAGAAT‑AAAATG‑AGAGAG‑AGTGTTGCT‑AAATTT‑ACCCCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGGGGAT‑AAAATAAAACAGCtca                                                                                                                                                                  >  1:567210/1‑237 (MQ=255)
                                                                        cgTTGATTATCAATCTGTTAATTGCTTCATAAGGGAGGAAAAAAATATAAAACCACATTTTTATTGTTAGTTGTTGGCGGT‑T‑AATTTTACTAAACAAATCAAAAAATTGGAT‑TGGCCAGGATGACAGAAAGAAACAGGAAAAAGGCCGTAAAT‑AAGTTG‑CCAGAG‑ATTGTCGCT‑ATTTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTaaa                                                                                                                                                                 <  1:1181395/239‑1 (MQ=255)
                                                                                  cGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAG‑TTGCAATGATGAGCAAAAGAAATAAGCAACAGGTAGTGAAT‑AAGTTG‑AAAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                                       <  1:894167/239‑1 (MQ=255)
                                                                                   gaccttttAATTACATTTTCACGGTGGAAAAAAATATAAACCCACATTTTTAGGGGTAGTTTGGGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAAT‑‑AGTTGAACAGAG‑ATGGTCGCT‑ACCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGaaa                                                                                                                                                      <  1:31673/233‑1 (MQ=255)
                                                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCCGACAAAAAAAAAAAATTGGG‑TTGCCATGATGGAGGGAAGAAATAAAAAAAAAGCAGTGAAT‑AAAGTG‑TGAGAGAATTTAGGTT‑AAATTT‑A‑CGCCAT‑GATTTCTT‑TCCCAAAGCCCCATGAAGGGGGGGGTAaaacaaaaacacaaaaagaggccggcacgca                                                                                                                                                   >  2:1488506/1‑219 (MQ=255)
                                                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAAACAAAAAAAAAAAATGGGG‑GTGTCATGATGAGGGGAAAAAATAAACAAAAGGAAGTGAAT‑AAGTTG‑AAAGAG‑AATGTCGCTAAAATTT‑ACC‑CCAT‑GATTTTTA‑CCCGAAAACCTGCTGACGTGGTGGAT‑AAAAAAAAAAAGCTAAAAGATGCCGacaacg                                                                                                                                                   >  1:425549/1‑233 (MQ=255)
                                                                                         ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCACACAAAAACAAAAAAAGGAG‑TTGCCCTCATGAGGGGAAAAAAAAAAAAAAAAGCAGCGAAA‑AAGTTG‑AAAGAG‑ATTGTGGCT‑AACTTT‑ATACCCAT‑GATTTTTT‑ACCCAATGCCTGTTGTAGGGGTGGGG‑AAACCAAAAAAGATTAAAGAAGGCGaaaattgg                                                                                                                                                 >  2:819420/1‑233 (MQ=255)
                                                                                         ttAATTACATATTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGAAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                    <  1:425536/204‑1 (MQ=255)
                                                                                                 atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGACAAAAAAAAAAACTGGAG‑GTGTCTTGATGAGGGGAAGTAAAAAACAAAAGGGAGAGAAT‑AAGTTG‑ACAGAG‑ATTTTCGCT‑AACTTT‑ACCGCCCT‑GATTTCTT‑CCCGCATGCCTGATGACGTGGTGGAT‑AAACTAAAACAACTAAAGAATGCCGAAACGACGTC‑GGAgggg                                                                                                                                       >  2:91766/1‑239 (MQ=255)
                                                                                                  tatgccgggTGGCAAAAAAGAAAAAACCACATTTTAAGGTGTTGTTGGTGGCGTG‑T‑AATTTTTCCAGACAAACACAAAAACTGGAG‑TTGCCATGATGAGGTAAAGAAAAAAGCAACAGGCAGTTAAA‑AGTTTG‑ACAGAG‑TTTGTGGCT‑ATCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  2:903177/232‑1 (MQ=255)
                                                                                                  tatTCGCGGTGAAAAAAAATAAAAAACCAAATTTTAAGTGGTAGTTTGTGGCGGT‑G‑AATTTTCCAAGACAAAAAAAAAAACGGGAG‑TTGCCTGGATGAGCGAAAGTAATAAGCAAACGGCAGTGAAT‑AATTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  2:687815/239‑1 (MQ=255)
                                                                                                  tatCCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGT‑A‑AATTTTCCCAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  1:813275/239‑1 (MQ=255)
                                                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑G‑AATTTTTTCAAACAAATAAAAAAAAAGGAGATTGCC‑TGATGAGAGAAAACAATAAAAAAAAAACAAGGAAA‑AAATTT‑AACGAG‑ATTGTGCCT‑AACTTT‑TCCACCCT‑AGTTTTTA‑CCCCAAAGCCCGATTGCCTGGGGGGT‑AAAAAAAAAAAACCAAAGGAAGACGaaaacccagcggtggggga                                                                                                                                      >  2:822932/1‑223 (MQ=255)
                                                                                                          tgggaaaaaaaaaTAAAACCACTTTTTTAGTGGTAGTTTGGGGGGGG‑G‑AATTTTTACAGACAAATACAAAAACTGGAG‑GTGCCATGATGAGGGAAAGTAATAAGCAACAGGCATTAAAT‑AAGTTG‑ACAGAG‑ATTTCCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  2:853033/233‑1 (MQ=255)
                                                                                                          tgggaaaaaaaTAAAAAACCCAATTTTAAGTGTGAGTTTGTGGGTGT‑A‑AATTTTCCCAGACAAATAAAAAACCGGGAG‑TTCCCTGGAGGAGGGAAATTAATAAGCAAAAGACATTAAAG‑AAGTGG‑ACAGAG‑ATTGTCGCT‑ATCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  2:513031/233‑1 (MQ=255)
                                                                                                          ggggaaaaaaaTATAAAACCACATTTTTGGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTGG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  2:267806/233‑1 (MQ=255)
                                                                                                          gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAATGGGG‑TTGCCATGATGAGCGGAAGTAATAAAAAACAAGCAGAGAAT‑AAATTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑TCCGCCAT‑GATTTTTA‑CCCCAAAGCCCGATGACGGGGGGGGT‑AAAATAAAACAGCTAAAAGATGTCGCAAAGTCGCC‑GGGGGGGGAAATTa                                                                                                                                >  1:513765/1‑237 (MQ=255)
                                                                                                          gTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGT‑G‑AATTTTTCCAGACAAATACAAAAACGGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  1:1389957/238‑1 (MQ=255)
                                                                                                           tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCCGACAAAAACAAAAACTGGAG‑TTTCCATGATGAGCGAAAGTAAAAAACAACAGGCAAAGAATAAAGTTG‑‑CAGAG‑ATTGTCGCT‑AAATTT‑ACCGCCAT‑GATTTTTT‑CCCCAATGCCCGATGAAGTTGGGGGT‑AAAATAAAAAAACTAAAAGGTGCCGAAAACGCGTC‑GGTGGGGGAAAATATCt                                                                                                                             >  2:224749/1‑239 (MQ=255)
                                                                                                           tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAAACAAAAAAAAAAAATGGAG‑GTGTCATGATGAGCGGAAATAATAAACAAAAAGCAAGGAAT‑AAAATG‑ACAGAG‑ATTGTTGCT‑AACTTT‑ACCGCCAT‑GATTTTTACCCCCAATG‑CTTATGGCGGGGGGGGT‑AAAACAAAACAGCTAAAAGGAGCCGAAAAGGCCTCGGAGGGGGAAAAtatat                                                                                                                              >  2:628479/1‑235 (MQ=255)
                                                                                                            agaaaaaaaTATAAACCCACATTTTTAGTGGTAGTTTGGGGCGGT‑A‑AATTTTTCAAAACAAAAACAAAAACTGGAG‑TTGCCATGATGAGGAAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGTT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTa                                                                                                                            <  2:348892/236‑1 (MQ=255)
                                                                                                             gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AAATTTTTCAAACAAATAAAAAAAATGGAG‑TTGGCATGATTAGCGAAAGGAATAAAAAAAAGGCAGGGAAT‑AAATTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCCT‑GATTTTTA‑CCCCAAAACCTGATGAAGTGGGGGGT‑AAAATAAAACAACTAAAAGAAGCCCAAAACTCGTC‑GGTGGGGGAAAATATTTa                                                                                                                            >  1:405616/1‑238 (MQ=255)
                                                                                                              cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTTCAGACAAAAAAAAAAAATGGAG‑GTGGCATGATGAGCGGAAGTAAAAAACAAAAAGCAGAGAAT‑AAAATG‑ACAAAG‑ATTGTCGGC‑AACTTT‑TACACCCT‑GATTTTTT‑CCCCGATGGCTCATGAAGTGGGGGGT‑AAAATAAAAAAACGAAAAGAGACCGAAAAGTCGCC‑GGTGGGGGAAATTATATAcc                                                                                                                          >  2:1389957/1‑239 (MQ=255)
                                                                                                                 aaaaTATAAACCCAAATTTTAAGTGGTAGTTTGGGGCGGT‑A‑AATTTTCCCAAAAAAATAAAAAAACTGGAT‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATa                                                                                                                       <  1:303345/239‑1 (MQ=255)
                                                                                                                   aaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                                                     <  1:856817/239‑1 (MQ=255)
                                                                                                                   aaTAAAAAACCACAGTTATAGTGGTAGTTTGTGGCGGT‑A‑AATTTGCCAAGAAAAAAACAAAAACGGGAG‑TTGCAATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCTCA‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                                                     <  2:732931/239‑1 (MQ=255)
                                                                                                                       taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGG‑AATTTTTCC‑GACAAAAAAAAAAAATGGAG‑TTGGCATGTTGGGGGGAAAAAATAAACAAAAAGCAGTGGAT‑AAAATT‑AAAGAA‑AATGTTGCT‑AAATTTAACCCCCAT‑‑ATTTTTA‑CCCCAATGCCCGATGACGGGGGGGGG‑AAAAAAAAAAAGCTAAAAGATGGCGGAACATCGGC‑GGTGGGGGAAAATATATAACATAcaaagt                                                                                                                 >  2:837292/1‑234 (MQ=255)
                                                                                                                               caTTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTCAGACAAAAACAAAAAATGGGG‑TTGCCATGATGAGGGAAAGAAATAAGCAACAGGCAGTGAAA‑AAATTG‑AAAGAG‑ATTGTCTCT‑AACTTT‑ACCGCCCT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGGT‑AAAATAAAAAAGCTAAAGGATGCCGAAAAGTCGTC‑GGTGGGGAAAATTATATAACATCAGATTTTCGacaac                                                                                                         >  1:1188013/1‑239 (MQ=255)
                                                                                                                               caTTTTTAGTGGTAGTTTGTGGCGGT‑G‑GATTTTTTAAAAAAAAAAAAAAAAATGGAG‑TCGCCATTATGAGGGGAAGGAATAAAAAACAAGCAGGGAAA‑AAAATT‑AAAAAG‑GTTGTCGCT‑AACTTT‑TTCGCCCTGGTTTTTTT‑CCCGAA‑GCCTGATGGAGTGGGGGGT‑AAACTAAAAAAACTAAAAGAAGCCGAAACAGCGTT‑GGTGAGGGAAAATATTTACCATaacgtgtttgacgac                                                                                                         >  2:257975/1‑225 (MQ=255)
                                                                                                                                        tgggattttGTGGGGGT‑G‑AATTTTTCCAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                                                 <  2:712410/232‑1 (MQ=255)
                                                                                                                                            ggttggggggggT‑A‑AATTTTACAAAAAAAATAAAAAACCTGGAG‑TTGCAATGATGAGCGAAATAAATAAGCACAAGGCAGTAAAT‑AAGTTG‑ACAGAG‑TTTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                            <  2:425549/229‑1 (MQ=255)
                                                                                                                                                   gtgcatgtaaaTTTTACCAAACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTATAAAGCAACAGGCAGTAAAT‑AAGTTG‑AGAAAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                                       <  1:382967/230‑1 (MQ=255)
                                                                                                                                                                ttccaaGAAAAATACAAAAATTGAAG‑TTGCAATGAGGGGAGAAAGAAAAAACCAACAGGCAGTGAAT‑AATTTG‑ACAGAG‑ATTGTCCAT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          <  1:579119/234‑1 (MQ=255)
                                                                                                                                                                      gACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                                                    >  2:679607/1‑239 (MQ=255)
                                                                                                                                                                       aaaaaaaaaaaaaCTGGAG‑TTGCAATGATGAGCAAAAGTAAAAAGCAAAAGGAAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc                                                                            <  2:1222728/222‑1 (MQ=255)
                                                                                                                                                                                 aaaCTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                  >  1:1222728/1‑230 (MQ=255)
                                                                                                                                                                                 aaaCTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                         >  1:951176/1‑239 (MQ=255)
                                                                                                                                                                                 aaaCTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                         >  1:819506/1‑239 (MQ=255)
                                                                                                                                                                                  aaCTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGAT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:1465131/239‑1 (MQ=255)
                                                                                                                                                                                  aaCTGGAG‑TTGCCAGGAGGAGCGAAAGAAAAAAGCAACAGGCAGTGAAT‑AAGTTG‑AAAGAG‑ATGGCCGCT‑AACTTA‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:454149/239‑1 (MQ=255)
                                                                                                                                                                                  aaCGGGAG‑TTGCAATGAGAAGGAAAAGTAATAAGCAACAGGCAGGGAAT‑AAGTTA‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  2:513765/239‑1 (MQ=255)
                                                                                                                                                                                   aCTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCGTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                                       >  1:347329/1‑239 (MQ=255)
                                                                                                                                                                                             gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCACAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                               >  2:93872/1‑238 (MQ=255)
                                                                                                                                                                                             gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                               >  1:1294000/1‑238 (MQ=255)
                                                                                                                                                                                             gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                               >  2:1114524/1‑238 (MQ=255)
                                                                                                                                                                                               cATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttct                                            <  1:837292/239‑1 (MQ=255)
                                                                                                                                                                                                       acaaaaGTAAAAAGCAACAGGCAGTAAAT‑AAGTTG‑AAAGAG‑ATTGTCTCT‑AATTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa                                    <  1:819420/236‑1 (MQ=255)
                                                                                                                                                                                                        cGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:175359/1‑239 (MQ=255)
                                                                                                                                                                                                        cGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:574027/1‑239 (MQ=255)
                                                                                                                                                                                                        cGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:961929/1‑239 (MQ=255)
                                                                                                                                                                                                         aaaaGTAAAAAGAAACAGGCAGTGAAT‑AAGTTG‑AAGGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa                                    <  2:405616/236‑1 (MQ=255)
                                                                                                                                                                                                            aGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                               >  1:958413/1‑239 (MQ=255)
                                                                                                                                                                                                                  aaGCAACAGGCAGGAAAA‑AAGTTG‑AAAGAT‑ATTGCCGCT‑AAATTC‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg                         <  2:306282/239‑1 (MQ=255)
                                                                                                                                                                                                                      aaCAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑CCCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCt                       <  1:91766/237‑1 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGCGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  1:303793/1‑239 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                   >  2:264252/1‑207 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:1367610/1‑239 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:918417/1‑239 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:172669/1‑239 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                    >  2:1097579/1‑238 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATACCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGCGGAGATTATGTTCTTCGTTACAGTCGGTTCCCGCTTccc                   >  1:1526525/1‑239 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AGCTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          <  1:1010147/184‑1 (MQ=255)
                                                                                                                                                                                                                        cAGGCAGTAAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                   <  1:264252/207‑1 (MQ=255)
                                                                                                                                                                                                                         gggaatagAAT‑AAGTTG‑AAAGAG‑AGTGCCACT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa                  <  1:864913/232‑1 (MQ=255)
                                                                                                                                                                                                                             aGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaa                                                                                                   >  1:1438642/1‑154 (MQ=255)
                                                                                                                                                                                                                             aGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACt                >  1:98148/1‑237 (MQ=255)
                                                                                                                                                                                                                                   t‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTA‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  1:845919/239‑1 (MQ=255)
                                                                                                                                                                                                                                      atttG‑AAAAAG‑TTTGTCGTT‑AAGTTA‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg      <  2:958413/237‑1 (MQ=255)
                                                                                                                                                                                                                                          g‑ACAGAG‑TTGGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  <  1:766642/239‑1 (MQ=255)
                                                                                                                                                                                                                                          g‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  1:1185212/1‑239 (MQ=255)
                                                                                                                                                                                                                                          g‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:257644/1‑239 (MQ=255)
                                                                                                                                                                                                                                          g‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:1399969/1‑239 (MQ=255)
                                                                                                                                                                                                                                          g‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGACTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCTCACTGCTTGGCGAGCTGc  >  1:1132289/1‑239 (MQ=255)
                                                                                                                                                                                                                                          g‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGGGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:1261735/1‑239 (MQ=255)
                                                                                                                                                                                                                                             cAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  1:440884/105‑1 (MQ=255)
                                                                                                                                                                                                                                             cAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      >  2:440884/1‑105 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
TGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑G‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAG‑ATTGTCGCT‑AACTTT‑ACCGCCAT‑GATTTCTA‑CCCGAATGCCTGATGACGTGGTGGAT‑AAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206272‑3206740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑