BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,760,383	A→C	24.7%	V289G (GTG→GGG)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,760,383	0	A	C	24.7%	174.5 / 17.6	108	V289G (GTG→GGG)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand): ref base A (58/20); new base C (2/27); total (60/48)
Fisher's exact test for biased strand distribution p-value = 1.45e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACG  >  NC_000913/3760146‑3760615

gCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATACTTCCTGCCTGCGGCCCGCCTCACAA‑CTTGTCGCCGGAG‑AAAATCa                                                                                                                                                                                                                                           >  1:1454305/1‑238 (MQ=255)

gCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCCCCGGGG‑AAACTCa                                                                                                                                                                                                                                           >  1:1232468/1‑238 (MQ=255)

     gattggattgACCTCCGCGCTGCGGGTAGGCAGGCGCAGGGGCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGa                                                                                                                                                                                                                                      <  2:1169943/238‑1 (MQ=255)

     gATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCCTCTCTTTTTT‑TCCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTTTCGCCGGAG‑AAACTCACCat                                                                                                                                                                                                                                       >  1:207969/1‑236 (MQ=255)

                cccctgcgcggcgGGTAGGCGGGGGCAGGGTCGGTAATTTTTCCCCCAGGGCTTCAGGGTGTAAATAAAGCCGCAACGGGGCTCCCAGCGCGGCGAGGGTCATTTTACCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTGGCGGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATCCCTGCCTGCGGCCCGCCTAAAAA‑CTTGTCGCCGGAG‑AAACTCCCCAGACTG‑Acgcccgc                                                                                                                                                                                                                          <  2:683689/235‑1 (MQ=255)

                 cgcggcggtgggGGTAAGCAGGCGCGGGGTCGGTAATTTTTCACTCAGGGCTTAGGGGTGAAAATAAAGACGCACCGGGGCTTCCAGCGCCGGGAGGGTCTTTTTACCCGCGCG‑TAATGCCCGCTTCAGCGGGGGGCTTGGCAGGCGGGCGATCATCTCTTTTTA‑ACAAACAATAATCCCTCCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCCCCAGACGG‑ACGCccgccg                                                                                                                                                                                                                        <  2:1038854/235‑1 (MQ=255)

                    tgcgctgcgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCCTTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCCTCTCTTTTTT‑TCCAACAATAATTCCTGCCCGCGGCCCGCCCCACAA‑CTTGTCGCCGGCG‑AAACTCACCCGACTG‑GCGCCCGCCGCaa                                                                                                                                                                                                                     >  2:727566/1‑239 (MQ=255)

                           cgGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATTCTCTCTTTTTT‑ACCAACAATAATTCCTGCCTGGGGCCCGCCTAACAA‑ACTGTCGCCGGAG‑AAACTAACCAGAcccaaccccccccccaccaact                                                                                                                                                                                                                >  2:1136850/1‑217 (MQ=255)

                            gggTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACCA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTc                                                                                                                                                                                                              >  1:583147/1‑238 (MQ=255)

                                aggcgggCGCAGGTTCGGTATTTTTGCTCACTGAGCTGCAGGTGTTAAAAAAAGACGCAACGTGGCTTCCAGCGCCGTGAGGGTCTTTTTATCCGCGCG‑TAATGCCCGCGTCGGCGGGTGGCTTTGCGGGCGGGCGATCATCTCTTTTTT‑ACCAACAATATTTCCTGCCTGCGGCCCGCCTAACAC‑CTTGTCGCCGGAA‑AAACTCCCCAGTCTG‑ACGCCCGCCGCAATCAACTCCTGc                                                                                                                                                                                                          <  2:50506/233‑1 (MQ=255)

                                aaGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTCACAA‑CTTGTCCCCGGAG‑AAACTCACCAGACTG‑GCGCCCGCCCCCATACACTCCTGc                                                                                                                                                                                                          >  2:104499/1‑238 (MQ=255)

                                            ggTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCCTTTTT‑ACCAACAATCATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCCCCGCCATCACCTCCTGCGGCCTTGGCCCtt                                                                                                                                                                                             >  2:66479/1‑239 (MQ=255)

                                             gTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCtt                                                                                                                                                                                             <  1:1198997/238‑1 (MQ=255)

                                                ggTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCg                                                                                                                                                                                          >  1:458922/1‑238 (MQ=255)

                                                ggTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCCGCGGCCCGCCCAACAA‑CCTGTCCCCGGAG‑AAACTCACCAGACTC‑ACGCCCGCCGCCATCAACTCCTGCGGCCTTGGcccgtttg                                                                                                                                                                                          >  2:1516540/1‑231 (MQ=255)

                                                       tttCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACCCCTGCGGCATTGGCTCTTTCGGCAAACCg                                                                                                                                                                                  >  1:225559/1‑239 (MQ=255)

                                                         tCACACAGAGCTTCGGGGTGAAAAAAAAGCCGCACCGTGGCTCCCGGCGCCGCGGGGGTCATTTTCCCCGCGCG‑TAATGCCCCTTCCAGCGGTTGGTTTGGCAGGCGGGCGATCATCTTTTTTTT‑CCCAACAATAATTCCTGCCGGCGCCCCGCCTAACAA‑CTTGTCGCCGGAG‑AACCTCACCAGCCTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                <  2:683330/239‑1 (MQ=255)

                                                         cacccaggacctatggggTGTAAAAAAAGCCTCATAGGGCCTGCCAGCGCCGCGAGGTTTTTTTTTCTCCCGCG‑TAATGCGCGTTTAAGCGGGTGGTTTTGCAGGCGGGTGTTCATCTCTTTTTT‑CCCAACATTATTCCCTGCCTGGGGCCCGTCTAACAT‑CTTGTCGCCGGAG‑AAACTCCCCAGACGG‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTa                                                                                                                                                                                <  2:874982/224‑1 (MQ=255)

                                                            cTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTg                                                                                                                                                                             >  1:1269527/1‑239 (MQ=255)

                                                            cTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTg                                                                                                                                                                             >  1:74625/1‑239 (MQ=255)

                                                               agagCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCCACTCCTGCGGCATTGGCTCTTTCCGCAAACCGTACTGGCt                                                                                                                                                                          >  1:609991/1‑239 (MQ=255)

                                                                gagCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCCACAATAATTCCTGCCTGCGGCCCCCCTAACAA‑CTTTTCGCCGGAG‑AAACTCACCCCACTT‑ACGCCCGCCCCAATCAACTCCTCCGGCATTGGCCCTTTCTGCAAACCCTACTGGcca                                                                                                                                                                         >  2:205423/1‑237 (MQ=255)

                                                                gagCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCCTACTGGCTa                                                                                                                                                                         >  1:814006/1‑239 (MQ=255)

                                                                 agCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACCA‑CTTGTTCCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCCAATCCTACGGCATTTGCACTTTCGGCAAAcccggccgggtaa                                                                                                                                                                        >  2:1056312/1‑228 (MQ=255)

                                                                      cggggTGTAAATAAAGCCGCAACGTGGCTTCCAGCGCCGGGAGGTTCTTTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCg                                                                                                                                                                   <  1:1052411/237‑1 (MQ=255)

                                                                      cAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCg                                                                                                                                                                   >  1:689084/1‑239 (MQ=255)

                                                                       aGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCg                                                                                                                                                                   >  1:263520/1‑238 (MQ=255)

                                                                           tgtAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCCATCAACTCCTGCCGCATTGGCTCTTTCCGCCAACCGTACTGGCTAACATCGACCa                                                                                                                                                               >  2:881061/1‑238 (MQ=255)

                                                                           tgtAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCa                                                                                                                                                               >  1:186982/1‑238 (MQ=255)

                                                                             taaataaaGACGCAACGTGGCTTCCAGCGCCGCGGGGGTCATTTTATCCGCGCG‑TACTGCACGCTTCAGCGGGGGGCTTTGCAGGCGGGGGAACATCTCTTTTTT‑ACCAACAAAAATTCCCACCCGCGACCCGTCTAACAC‑CTTGTCGCCCCGG‑AAAATCACAAGAGTC‑CAGCCCACCCCAATCCACTCTTGGCGCATTTGCTCTTTGCGCAAACCCTACTAGCTAAGTTcctcctgcg                                                                                                                                                            >  2:1014155/1‑231 (MQ=255)

                                                                             taaataaaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCg                                                                                                                                                            >  1:214116/1‑239 (MQ=255)

                                                                                  aagggcgaaagggggCTCCCGGCGCCGGGTGGGTCATTTTACCCGCGGT‑AAAGGCACGCTTCGGCGGGGGGTTTTGAGGGGGGGCAATCATCTCTTTTTA‑ACCAAAAAAAATCCCTGCCGGCGGCCCGCCTAACAC‑CTTGCCGCGGGAG‑AAACTCCCCAGACGG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCa                                                                                                                                                       <  2:647539/226‑1 (MQ=255)

                                                                                   aaGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGAACAGCGAGcc                                                                                                                                                        >  1:1158534/1‑237 (MQ=255)

                                                                                           aCGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCGACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCCGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTaaa                                                                                                                                              >  1:1524033/1‑239 (MQ=255)

                                                                                                 ctcccggcgcCGGGAGGGTCATTTTCCCCGCGCT‑TAATGCACGCTGCAGCGGGTGCTTTTGCGGGCGGGCGATCTTCTCTTTTTC‑ACAAAAAATAATCCCTGCCGGCGGCCCGCCAAACAA‑CTTGTCCCCGGAA‑AACCTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGt                                                                                                                                         <  2:609991/232‑1 (MQ=255)

                                                                                                 ctcccggcgcCGCGAGGTTTTTTTTACCCGCGCT‑TAATGCACGCTTCAGGGGGTGGCTTTGCAGGCGGGCCATCATCTTTTTTTT‑ACAAAAAATATTCCCTCCCTGCGCCCCGCCTAACAA‑CTTGGCGCGGGAG‑AACCCCCCCAGACTG‑CCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGt                                                                                                                                         <  2:74625/232‑1 (MQ=255)

                                                                                                 ctcccAGCGCCGGGGGGGTTATTTT‑TCCCCGCGTTAATCCACGCTACGGGGGGGGGCTTGGCAGGCGGGCGATCATCTTTTTTTA‑CCCAACAATATTCCCTGCCGGCGGCCGCCTTAAAAC‑CTTGCCGCGGGGA‑AACCTCCCACGACGG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGt                                                                                                                                         <  2:1524033/235‑1 (MQ=255)

                                                                                                    ccAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGGG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCCCTTTCGGCAAACCGTAATGGCTAAGATCGACCAGCGAGCCACTGCCTAAATAAGTCACt                                                                                                                                     >  1:1352572/1‑239 (MQ=255)

                                                                                                          ccgcggtcgTCTTTTTCTCCGCGTT‑TAGTCCCCGTTTCGGCGGTGCGCTTGGCTGGCGGGGTGTCTTCTTTTTTTC‑ACCAAAAATACTCCCGGCCGGCGGCCCGCCTAACAC‑CTTGTC‑CCCGAG‑AAACCCCACTCACCGCCCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGa                                                                                                                               <  2:146694/231‑1 (MQ=255)

                                                                                                           cgcgTGGGTCATTTTACCCTCGTG‑TAATGCCCGCTCAAGGGGGTGGCTGTGCCGCGGGGCTTTCCTCTCTTTCTT‑CACAACAAAATTTCCTGCCGGCGGCCCGCCTAACAA‑CTTGTCGCCGGAA‑AAACTCCCCAGCCGG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGa                                                                                                                               <  2:88591/238‑1 (MQ=255)

                                                                                                             cgcgggTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGGGATCCTCTCTTTTTT‑TACCACCATAAATCCTGGCTGCGGCCCGCCTACCAA‑CTTTTCGCCGGAG‑AGACGCACCAGACTT‑AACCCCGCCGCACTCAACGCCTGCGGCAGTGGCTCTTTCGGCCAAACGGAACCGCTAACATACAACAGCCAGCCAGTGCCTAAATCAATCAcaccgcgcaa                                                                                                                             >  2:638349/4‑229 (MQ=255)

                                                                                                             cgAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGGTCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGGG‑AAACTCACCAGCCTG‑ACGCCCGCCGCCATCAACTCCTGCGGCATTGGCTCCTTTCGCAAAACCTACCGGCTAAGATCCACCAGCGCGCCCCCGCCCACATACGCCCCTACgggga                                                                                                                              >  2:643475/1‑235 (MQ=255)

                                                                                                                               cgcg‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCCAAGATCGCACAGCGAGCCACTGCCTAAATCAGTCAACACGGGAACATCCAGCTCTTTGCCggg                                                                                                           >  1:1410947/1‑236 (MQ=255)

                                                                                                                               cgcg‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAACAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCCGCG‑AAACTCCCCAGACCG‑ACGCCCGCCCCCATCAACTCCTCCGGCATTTGCTCTTTCGGCCAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCCCTACGGGGACATCCAACTCTTTTCCgcgc                                                                                                          >  2:1243024/1‑239 (MQ=255)

                                                                                                                                           cccTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTTTTTTTT‑CCCAACAATAATCCCTGCCGGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCCCCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTc                                                                                               <  1:719157/237‑1 (MQ=255)

                                                                                                                                             cTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACCGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTcc                                                                                              >  1:491669/1‑238 (MQ=255)

                                                                                                                                               tCAGCGGGTGGCTTTGCAGGCGGGCGCTCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCCCTTTCGGCAAAACGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACCACGGGAACATCCCGCTCTTTGCCGAGCGCCACCAGTTCCGCt                                                                                           >  1:566615/1‑239 (MQ=255)

                                                                                                                                                 aGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCCACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCtt                                                                                          >  2:1219254/1‑238 (MQ=255)

                                                                                                                                                       gggttttGCAGGCGGGCGATCATCTTTTTTTT‑ACCAACAATAATTCCTGCCGGCGGCCCGCCAAACAC‑CTTGTCGCGGGAG‑AACCTCCCCAGCCTG‑CCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTa                                                                                    <  1:104499/234‑1 (MQ=255)

                                                                                                                                                        gtcTTTGCAGGCGGGCGATCTTCTCTTTTTT‑ACCAACAATAATCCCTCCCTGCGGCCCGCCTAACAA‑CTTGCCGCCGGAA‑AAACTCCCCAGATGG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTAt                                                                                   <  1:988164/236‑1 (MQ=255)

                                                                                                                                                        ggCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCCAACCGTACTGGCCAAGATCGACCAGCGAGCCACCTCCTAAATCAGTCAATACGGGGACATCCAGCTCTTTGCCGCACGCCCCCAGTTCCGCCTCCTCCAt                                                                                   >  2:551359/1‑238 (MQ=255)

                                                                                                                                                        ggCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATc                                                                                  >  1:1121549/1‑239 (MQ=255)

                                                                                                                                                          cTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATc                                                                                  >  1:996052/1‑237 (MQ=255)

                                                                                                                                                               agggggcgcgATCATCTTTTTTTC‑ACCAACAAAAATCCCTGCCTGCGCCCCGCCTAACAC‑CTTGCCGCGGGAA‑AACCCCACCAGACGG‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTg                                                                            <  2:214116/231‑1 (MQ=255)

                                                                                                                                                                aGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGt                                                                          >  1:1324100/1‑239 (MQ=255)

                                                                                                                                                                  gcgggcgATCATCTCTTTTTC‑ACCAACAATAATCCCCGCCGGCGGCCCGCCTAACAA‑CTTGTCGCGGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg                                                                         <  2:263520/238‑1 (MQ=255)

                                                                                                                                                                    ggtcgATCTCCTTTTTTTC‑ACCAAAAATACTCCCCGCCGGCGCCCCGCCTAACAA‑CTGGCCGCCGAAA‑AACCCCCCCAGACTG‑CCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCCAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGaa                                                                       <  2:1158534/235‑1 (MQ=255)

                                                                                                                                                                     ggcgATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAAc                                                                      >  1:556377/1‑238 (MQ=255)

                                                                                                                                                                     ggcgATCATCTCTTTTTT‑AACAACAATAATTCCTGCCTGCGGGCCGCCTAACAA‑CTTTTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTTGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCACTCACCACCGGCACATCCACCTCTTTGCCGCGCGCCACCCGTTCCGCTTCCTCTATCCCTTTTGTGAAc                                                                      >  2:433952/1‑238 (MQ=255)

                                                                                                                                                                        gATCAT‑TCTTTTTTGACAAAAAAAAATCCCGCCCTGCGGCCCGCCTAACAC‑CTTGCCGCGGGAG‑AAACCCCCCGAGCGG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCt                                                                  <  2:409977/239‑1 (MQ=255)

                                                                                                                                                                           catcTCTTTTTT‑ACCAACAATAATCCCTCCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACCCCCCAGACTG‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGaa                                                               <  1:1257730/239‑1 (MQ=255)

                                                                                                                                                                           catcTCTTTTTT‑ACAAAAAATAATCCCGGCCGGCGGCCCGCCAAAAAA‑CTTGCCGCGGGAA‑AAACCCCCAAGCCTG‑CCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGaa                                                               <  2:1324100/239‑1 (MQ=255)

                                                                                                                                                                             tctcTTTTTT‑ACCAACAATCACCCCCGTCCGGGCCCCGCCTACCAA‑CTTGTCGCCGGAA‑AAACTCCCCAGACGC‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATg                                                             <  2:1256135/239‑1 (MQ=255)

                                                                                                                                                                             catatggtgaaaagaaccaaaatCCCTGCCGGGGCCCCCCCTAACAA‑CTTGCCCCCGGAG‑AACCCCCCCAGCCCG‑CCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCGCTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGaa                                                               <  2:740891/218‑1 (MQ=255)

                                                                                                                                                                                ctTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAAt                                                              >  1:399562/1‑235 (MQ=255)

                                                                                                                                                                                      t‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                     >  1:1374275/1‑238 (MQ=255)

                                                                                                                                                                                       tacaaacaaaatccccggcgggcGGCCC‑CCCAACAAGCTGGCCGCCGGAA‑AACCCCCCCTGACGA‑CCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                    <  2:814006/218‑1 (MQ=255)

                                                                                                                                                                                       cccaaaaaagaactcctccCGGGGGCCCCCCTAAAAT‑CTTGCCGCCGGAA‑AACCACACAAGACGG‑CCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                    <  2:772050/226‑1 (MQ=255)

                                                                                                                                                                                       caCCAAAAATAATCCCTGCCTGCGCCCCTCCTAAACA‑CTGTCCGGCGGAA‑AAACCCCCCAGACGG‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                    <  2:1374275/238‑1 (MQ=255)

                                                                                                                                                                                       accaaaaaaaacccccccccggcGGCCGGCCAACAAA‑CTTGCCGCGGGAA‑AACCCCCCAAAACTG‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTa                                                    <  2:1064975/218‑1 (MQ=255)

                                                                                                                                                                                        aCCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                     >  1:1192389/1‑237 (MQ=255)

                                                                                                                                                                                            acaaTAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGt                                               >  2:765015/1‑239 (MQ=255)

                                                                                                                                                                                               ataatTCCTGCCGGCGGCCCGCCTAACAA‑CTTGCCGCCGGAG‑AAACCCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATg                                            <  1:595089/239‑1 (MQ=255)

                                                                                                                                                                                                 acttCCGGCCGGCGGCCCGCCTAAAAA‑CTTGCGGCGGGAG‑AACCACACCAGACGG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                          <  1:1140385/237‑1 (MQ=255)

                                                                                                                                                                                                 aatCCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCCCCGGAG‑AACCTCCCCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                          <  1:1040957/239‑1 (MQ=255)

                                                                                                                                                                                                 aatCCCGGCCTGCGCCCCCCCTAACAC‑TTTGTCGCCGGAG‑AAACCCCCCAGCCTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTa                                          <  2:1063368/239‑1 (MQ=255)

                                                                                                                                                                                                   tccctgcctgCGGCCCCCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACt                                        <  1:551359/237‑1 (MQ=255)

                                                                                                                                                                                                   tccctccccgCGACCCGCCGACAAC‑CTTGTCGCCAGAG‑AAACCCACCAGACTC‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACt                                        <  2:583147/237‑1 (MQ=255)

                                                                                                                                                                                                      ctgcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtc                                     >  1:366442/1‑239 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGCG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACCTCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATGGCTTTGGTGAACCCCTGACTGCTGTAGTTATTGGTATGTACTTtca                                    >  2:750145/1‑238 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTg                                                                                                                >  1:175952/1‑162 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg                                                                         >  2:1405627/1‑201 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                    >  1:1536250/1‑238 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                    >  2:618349/1‑238 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtca                                    >  1:316565/1‑238 (MQ=255)

                                                                                                                                                                                                        gcctgcGGCCCGCCTAACAA‑CTTGTCGCCGGAA‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTg                                                                         <  1:1405627/201‑1 (MQ=255)

                                                                                                                                                                                                         cccgcgcCCCGCCAACAAA‑CTTTCCCCCGGAA‑AAACCCCCCAGACGG‑CCGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTtcatc                                  <  2:151029/236‑1 (MQ=255)

                                                                                                                                                                                                            gcGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCGCTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGtt                                                     >  1:740891/1‑217 (MQ=255)

                                                                                                                                                                                                             cGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                              >  2:1152883/1‑239 (MQ=255)

                                                                                                                                                                                                             cGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                              >  2:66399/1‑239 (MQ=255)

                                                                                                                                                                                                             cGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACa                              >  1:1468680/1‑239 (MQ=255)

                                                                                                                                                                                                              ggCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTCTGTCCTTTCATCACCa                              >  2:1224551/1‑238 (MQ=255)

                                                                                                                                                                                                                cccGCCTAACAC‑CTCGCCGCCGGAA‑AACCCCCCCAGACGC‑ACCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTg                           <  2:225559/239‑1 (MQ=255)

                                                                                                                                                                                                                cccGCCTAACAA‑CTTGCCGCCGGAG‑AAACCCACCAGACTG‑CCCCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTg                           <  2:329443/239‑1 (MQ=255)

                                                                                                                                                                                                                               tgaccgcGGAGAAAACCCACCAGACCC‑CCCCCCGCCGCAATCAACTCCGGCGGCATTGGCTCTTTCGGCAAACCGGACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcatt              <  2:1352572/233‑1 (MQ=255)

                                                                                                                                                                                                                                gTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTACGATCGACCAGCGAGCCACTGCCTACATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGACTGCTGTAGTTACTGGAATGTACTTTCATCAACACTGCGGTATTttcattc             >  1:599060/1‑238 (MQ=255)

                                                                                                                                                                                                                                gTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc             >  1:1027159/1‑238 (MQ=255)

                                                                                                                                                                                                                                gTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTttcattc             >  2:195880/1‑238 (MQ=255)

                                                                                                                                                                                                                                     cGGAG‑AAACTCTCCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGcc       <  1:1185643/239‑1 (MQ=255)

                                                                                                                                                                                                                                           aaaCTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACg  <  1:765015/239‑1 (MQ=255)

GCCTGGATTTGAATGACCTCTGCGCTGCGGGTAAGCAGGCGCAGGGTCGGTAATTTTTCACTCAGAGCTTCAGGGTGTAAATAAAGACGCAACGTGGCTTCCAGCGCCGCGAGGGTCATTTTATCCGCGCG‑TAATGCACGCTTCAGCGGGTGGCTTTGCAGGCGGGCGATCATCTCTTTTTT‑ACCAACAATAATTCCTGCCTGCGGCCCGCCTAACAA‑CTTGTCGCCGGAG‑AAACTCACCAGACTG‑ACGCCCGCCGCAATCAACTCCTGCGGCATTGGCTCTTTCGGCAAACCGTACTGGCTAAGATCGACCAGCGAGCCACTGCCTAAATCAGTCACTACGGGAACATCCAGCTCTTTGCCGAGCGCCACCAGTTCCGCTTCATCTATCGCTTTGGTGAACCCCTGAATGCTGTAGTTACTGGTATGTACTTTCATCAACAGTGCGGTATTTTCATTCACCGCCTGACG  >  NC_000913/3760146‑3760615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`