BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,830,355	T→A	13.2%	intergenic (+20/‑101)	xanP → / → yicH	xanthine permease/putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,830,355	0	T	A	13.2%	280.3 / 6.6	113	intergenic (+20/‑101)	xanP/yicH	xanthine permease/putative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand): ref base T (52/45); new base A (2/14); total (54/59)
Fisher's exact test for biased strand distribution p-value = 2.47e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

GTGCGTATCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTA  >  NC_000913/3830129‑3830589

gtgCTTGTCGTTTCTCGTGGGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCGGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACcgccgc                                                                                                                                                                                                                                <  2:1309358/239‑1 (MQ=255)

  gCGTATCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACcgccgccg                                                                                                                                                                                                                              >  1:389318/1‑237 (MQ=255)

   cGTATCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGGTTTTGCAGTTTGCCCCTG‑AA‑TGGCCGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGGTC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCCCCGCAg                                                                                                                                                                                                                              >  2:1455225/1‑238 (MQ=255)

   cGTATCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAg                                                                                                                                                                                                                              >  1:180971/1‑238 (MQ=255)

   cGTATCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGGG‑CTGAATCTGATTTTCCCACC‑AGCAAAA‑C‑AGTAATTCACCGGCGGCGGTAAGATAATTAcccacccag                                                                                                                                                                                                                              >  1:1100367/1‑231 (MQ=255)

      ctCGTTTCTCGTGGGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGGTGTCGCTGGCGGTTGGTCGGGGGGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGtctc                                                                                                                                                                                                                          <  2:1456642/238‑1 (MQ=255)

      aTCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTTACTCGCGGCGGGAAGAAAATTACCGACGCAGtttc                                                                                                                                                                                                                          >  1:292615/1‑239 (MQ=255)

      aTCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGGATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGGAAGATAATTACCGCCCCAGtct                                                                                                                                                                                                                           >  1:664256/1‑238 (MQ=255)

       tcttttCTCGTGGGCGGCGGAGCCGTGGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTATGGGGGTGTCTCA‑GCGGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGACTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCCCC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                                                                                                                         <  2:1190230/236‑1 (MQ=255)

       tcttttCGCGGGGGCCGCTGAACCGTCGGGCGATTTTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                                                                                                                         <  1:397276/236‑1 (MQ=255)

       tCGTTTCCGTTGAGCCGCTGAACCGTGGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑ACCCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGtctc                                                                                                                                                                                                                          <  1:190181/239‑1 (MQ=255)

       ccGTTTCCCGTGGGCCGCTGACCCGGCGGGCGATTCTGATTATCGCGCTGTCGGTGGCGGTTGGTCTGGGCGTTTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGGGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCCCC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                                                                                                                         <  2:908290/238‑1 (MQ=255)

        tgTTTCTCGTGGGCCGCGGAACCGTCGGGCGATTTGGATTATCGCGGTGTGGCTGGCGGTGGGTCGGGGCGTGTCCAA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑GGGCTGAAAA‑A‑CCG‑G‑CCCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGGG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTATTTCACTGGGGGCGGTAAGATAATTACCGCCGCAGTCTCAc                                                                                                                                                                                                                        <  2:1352185/238‑1 (MQ=255)

        gttttGTCGGGGGCCGCTGACCCGTCGGGCGATTTGGATTATGCCGCTGTCGCTGGCGGTTGGTCGGGCCGTGTTTCG‑CCAGCCGATGATTTGGCAGTTGCCCCCTG‑AC‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCGGGGATG‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGGGGCGGGAAGTTAATTACCGCCGCAGTCTCAc                                                                                                                                                                                                                        <  2:948057/237‑1 (MQ=255)

        gacagctggTGGGCCGCTGAACGGTGGGGCGATCCTGATTATCGCGGTGTCGCGGGCGGTGGGTCGGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCa                                                                                                                                                                                                                         <  2:1420051/230‑1 (MQ=255)

        cGTTGCTGGTGAGCTGCTGAACCGCGGGGCACTTCTGCTGATCGCGTGGTCGCTGGCGGTTGGTGTGGGTGTGTCCCA‑GCAGCCGTTGATTTTGCAGTTTGCCCCGG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTTTCCTCCGGGATC‑GCCGCGGGGGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAC‑A‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCAc                                                                                                                                                                                                                        <  2:1392014/239‑1 (MQ=255)

            tctcGGGAGCCGCTGACCCGGCGGGCTATTCTGATTATCGCGCTGTCGTGGGCGGTTGGTCTGGGCGTGTCCCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACctct                                                                                                                                                                                                                    <  2:1390576/239‑1 (MQ=255)

             ctcGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTTAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCCGCGGTAAGATAATTACCCCCGCACTCTCCCCTCtt                                                                                                                                                                                                                   >  1:366901/1‑239 (MQ=255)

                      cctggacccggggggCGTTTTTGTTTACCGCGCTGTCGGGGGGGGTTGGTTGGGGGGTGTCCCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AT‑TGGCTGAAAA‑C‑CCT‑G‑CTCCCCCCGGGGATC‑GCCGGGGGCGGTATTCCTG‑CCTTCGTG‑CTGAATTTGTTTTCCCCCCC‑AAAAAAA‑C‑ATTAATTCACGGGGGGCGGAAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTc                                                                                                                                                                                                          <  2:686667/227‑1 (MQ=255)

                        cTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGGAAGATAATTACCGCCGCAGTCTCACCTCTTTTAc                                                                                                                                                                                                               >  1:1420051/1‑232 (MQ=255)

                                tCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTTCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCCGTCTCCCCTCTTTAACCCTTCCCGCCTTGAg                                                                                                                                                                                                >  1:1508251/1‑239 (MQ=255)

                                     cGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCCCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGGtt                                                                                                                                                                                            >  1:1502380/1‑238 (MQ=255)

                                             aTTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGGGTCTCA‑GCAGCCGCTGTTTTTGCAGTTTGCCCCTG‑AA‑TGGCTGGAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCCTCGTG‑CTGAATCTGCTTTTCCCCCC‑CGCAAAA‑C‑CGTAATTCACTGGCGGCGGGAAGATAATTACCTCCCCAGTCCCCCCCCTTTAACCATTCCCCCCTTGAGGGTTTGGCGTaaa                                                                                                                                                                                   >  2:489324/1‑239 (MQ=255)

                                              ttATCGGGCTGTCGGTGGCGGTTGGGTGGGGCGTTCCTCA‑GCACCCTTGGTTTTTGCAGTTTGCCCCTG‑AA‑TGGCTAAAAA‑C‑GCT‑T‑CTCCCCTCTGGTATC‑CCCGCGGGCGTTATTACTC‑CCAT‑GTGTCTGAATTTTATTTTCCCCCC‑AAAAAAA‑C‑AGTAATTCACGGGCGGGGTTAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTaaa                                                                                                                                                                                   <  2:1389931/238‑1 (MQ=255)

                                                 tCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTTCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCCCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGGAAATCGt                                                                                                                                                                               >  1:565874/1‑239 (MQ=255)

                                                  cgcgGTGTCGCTGGGGGTGGGTTTGGGCGT‑TCTCACGCGGCCG‑TGGATTTGCAATTTGCCCCCGAAA‑TGGCGAAAAA‑C‑CCT‑G‑CCCCCCCCGGGGACC‑GCCG‑GGGCGGTTTTTCTGCCCATGGTC‑CTGATTCTGTTTTCCCCCCC‑AAAAAAA‑C‑ATAAATTCCCGGGGGGGGGTAAGTAAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTg                                                                                                                                                                              <  2:1508251/239‑1 (MQ=255)

                                                  cgcgCTGTCGCTGGCGGTTGGGCTGGGCGCGTATCC‑GCAGCCGCTGATTTTGCCGTTTGCCCCCG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGGTC‑GCCGCGGGCGGCATTACTG‑CCCTCGTG‑CTGAATCTGATTTTCCCACC‑AGAGAAA‑C‑AGTAATTTAACGGCCGCGGGAAGTAAATTACCGCCGCAGACTCACCTCCTTTACCCATCCCACCTTTAGGATTGCGCGCaaaacct                                                                                                                                                                               >  2:234096/1‑234 (MQ=255)

                                                    cggtggcctgcgCGGTTGGTTGGGATGTTTCTAA‑CCAGCCGTGGTTTTAGCTGTTCGCCCTCT‑AA‑TGCCTAAAAA‑C‑CCT‑G‑CTCTCCGCAGGCACG‑CCCGCGGGCGTTATTACTG‑CCAGCGTC‑TTTAACCTGATTTCCCCTCC‑AGAAAAC‑C‑CTTAATTCGCTGGCGGCGGAAAGATATTTACCGCGGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCa                                                                                                                                                                            <  2:1281027/228‑1 (MQ=255)

                                                       tGTCGCTGGCGGTGGGTCTGGGCGTGTCACA‑GCAGCCGTTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCGGGGACC‑GCCGGGGGCGGTATTACTG‑CATTCGTG‑CTGAATCTGATTTCCCCCCC‑AGAAAAA‑C‑AGTAATTCACGGGCGGCGGTAAGATAATTACCGCCGCAGTATCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATa                                                                                                                                                                          <  1:484421/238‑1 (MQ=255)

                                                        ggggcgggtggTTGGTCGGGGCGGTTCTCA‑CCACCCGTTGTTTTGCCAGTTTCCCCCTG‑AA‑GGGCTAAAAA‑C‑CCG‑G‑CTCTCCCCCGGAACC‑GCCGCGGGCGGTATTACTG‑CCAGCGTG‑GTAAATTTGATTTCCCCACA‑AAAAAAA‑A‑AGTATTTCACGGGGGGCGGTAGAATAATTCCCGCCGCAGTCTCCCCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATaa                                                                                                                                                                         <  2:238460/229‑1 (MQ=255)

                                                         tctcTGGGGGTTGGTCGGGGGGTGTCACA‑GCAGCCGCTGATTTTGAAGTTTGCCCCTG‑AT‑TGGCGGAAAA‑A‑CCT‑G‑CTCCCCTCCGGGAGC‑CCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGtctc                                                                                                                                                                                                                          <  2:713386/185‑1 (MQ=255)

                                                          cgtgggggtgggtgtgcggCGTGCCTCA‑GCCCCCGCTTATTTGCCAGTTTCCCCCGA‑AA‑GGGCT‑AAAA‑A‑CCTGG‑CTCTCCCCCGGGACC‑GCCGGGGTCGTTATTACTC‑CCTTGGTG‑TTAAATCT‑ATTTTCCCCCC‑AAAAAAACC‑ATTATTTCACGGGGGGGGGAAAAATATTTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACt                                                                                                                                                                       <  2:1135915/221‑1 (MQ=255)

                                                               gcgttgtgtgtGGGTGTGTCCAA‑GCGGCCCCTGTTTTTGC‑GGTTGCCCCTT‑AA‑AGGGCGAAAACA‑CCT‑G‑CTCCCCCCGGGGACC‑GCGCGGGGGGGTTTTACTC‑CCGTTGTG‑CTAAATTTATTTTTCCCCCC‑AAAAAAA‑C‑AGAATTTCGCTGGCGGCGGAAAAAAATTTCCCGCAGCAGTCTCACCTCTTTACCCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCtt                                                                                                                                                                  <  2:223517/228‑1 (MQ=255)

                                                                 gtgtgtcgtGGGGGTGCCTCA‑GCCGCCCCTGATTTTGC‑GTTTTGCCCGG‑AA‑AGGGTAAAAA‑ACCCT‑C‑CTCCCCCCCGGGACC‑GCCGCGGGGGGTATTCCTG‑CCATCGTG‑CTGAATCTATTTTCCCCACC‑AAAAAAA‑C‑ATTAATTCACGGGGGGGGGTAAGAAAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAt                                                                                                                                                                <  2:65836/230‑1 (MQ=255)

                                                                 ggttggttGGGGCTTGTCTCA‑GCAGCGCTTTATTTTGAAGTTCGCCCGTG‑AA‑TGGGTAAAAA‑C‑CCT‑G‑CTCCCCTCCGGTCGC‑GCCGGGGGGGGTATTACTC‑CCTGCGTG‑CTGAATCTTATTTTCCCCCA‑AAAAAAA‑C‑ATAAATTCCCTGGGGGCGGTAAGATATTTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAt                                                                                                                                                                <  2:1057255/238‑1 (MQ=255)

                                                                 gggtGGTCTGGGGGTGTCTCG‑GCGGCCGTTGATTTTGGA‑TTTGCCCCAG‑AAGTGCCTGAAAA‑A‑CCT‑G‑CTCCCCTCCGGGATC‑GCCGCGGGGGGTTTTACTG‑CCTTCGTG‑CTGAATCTGATTTCCCCACA‑AAAAAAA‑C‑AGAAATTCCCGGGCGGGGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAt                                                                                                                                                                <  2:982002/235‑1 (MQ=255)

                                                                   ttGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGGTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTaa                                                                                                                                                                                                                >  1:713386/1‑188 (MQ=255)

                                                                    tggggtGGGCGGGTCTCA‑CCAGCCGTGGTTTGTGGATTTGGCCCCGG‑AA‑TGGCTAAAAA‑C‑CCT‑G‑CTCCCCTCGGGGACC‑GCGGGGGGCGGTATTACTG‑CCATCGTG‑CTGATTGTGTTTTCCCCACA‑AAAAAAA‑C‑AGTAATTCCCGGGCGGGGGAAAGAAATTTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                                                                                                                             <  2:1459971/233‑1 (MQ=255)

                                                                    tGGTCGGGGCGTGTCTCA‑GCAGCCGCTGTTTTTGCATTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTCCCCACA‑AAAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTAtgtg                                                                                                                                                             <  1:1274068/238‑1 (MQ=255)

                                                                           ggtgtgcccca‑gcagCCGTTGTTTTGGCAGTTTCCCCTTG‑AG‑TGGCTAAAAC‑C‑CCT‑G‑CTCTCCGCGGGGACC‑GCCGCGGGCGGTATTCCTG‑CCATGGTG‑CTGAATCTGATTTCCCCACC‑AAAAAAA‑C‑AGTAATTCACTGGGGGCGTAAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCa                                                                                                                                                      <  2:1361343/229‑1 (MQ=255)

                                                                           gggtggtCTCG‑GCGCCCGCTTTTTTTGCAGTTGCCCCCTA‑AA‑GGGCGAAAAA‑C‑CCT‑C‑CCCTCCCCCGGGACG‑GCCGCGGGCGTTATTCCTG‑CCATCGTG‑CTGAATCTGATTTCCCCACA‑AAAAAAA‑C‑AGAATTTCACGGGGGGCGGTAAGAAAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCa                                                                                                                                                      <  2:664256/233‑1 (MQ=255)

                                                                           gctttttcgca‑gcagCCGCTGTTTTTGCAG‑TTGCCTCAG‑GA‑AGGGTTAAAA‑A‑CCC‑GTCTCCCCCCGGGGACC‑CCCGCGGGGGGTTTTCCTC‑CATTGGTG‑CTAAATTGGATTTCCCCCCC‑AAAAAAA‑C‑GGAAATTCACTGCGGGCGGTAAAAAATTTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCa                                                                                                                                                      <  2:214587/229‑1 (MQ=255)

                                                                            ggtgggtcgcggcagcCGCTGATTTGGCAGTTGGCCCGGA‑AA‑GGGCTGAAAA‑C‑CCT‑G‑CTCCCCCCGGGGAGC‑GCCGGGGGGGGTATTACTG‑CCAGCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGGGGCGGAAAGAAAAATACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCa                                                                                                                                                      <  2:548339/227‑1 (MQ=255)

                                                                                 tctcA‑GCAGCCGCTGATTTTGCCGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAACC‑C‑AGTAATTCACTAGCCGCGTGAAGATTATTACCACCCCAAACTCACCTCTTTTAGCTTTCCCCCCTTGGGGCTTTCGCGACAATCGTGCTTAACTCCCTTTTGTCCATTTTActgcgag                                                                                                                                               >  2:880926/1‑233 (MQ=255)

                                                                                     a‑gcagcCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGCTTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCCGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGCTTGGGGTTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGCTGGaa                                                                                                                                            >  2:927715/1‑238 (MQ=255)

                                                                                     a‑gcagcCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAg                                                                                                                                           >  1:669728/1‑239 (MQ=255)

                                                                                     a‑gcagcCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTCCATAACTCCCTTATGTGCATTTCACGGGATGGAAg                                                                                                                                           >  1:116599/1‑239 (MQ=255)

                                                                                     a‑gcagcCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCCTGAGGATTGCGCGTAAATCGTGCCTAACTCCCTTATGTGCATTTCACGGGATGGAAg                                                                                                                                           >  2:116068/1‑239 (MQ=255)

                                                                                         agcCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑C‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGGGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTCCCCACC‑AAAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGAc                                                                                                                                         <  1:1224670/238‑1 (MQ=255)

                                                                                              gtgtgtgggcattcgcccctTG‑AA‑TGTGTGAAAA‑C‑CCT‑C‑CTCTCCTCCGGAACC‑CCGGCGGCGGGTTTTCCTG‑CCAT‑GTT‑GTGAATTTGGTTTTCCCCAC‑AAAAAAA‑CAAGTATTTCACGGGGGGGGGTAAGATAATTACCGCCGCAGTCTCCCCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGaa                                                                                                                                   <  2:1195660/223‑1 (MQ=255)

                                                                                                gATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAAt                                                                                                                                 >  1:388143/1‑239 (MQ=255)

                                                                                                           ttGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGaa                                                                                                                       >  2:926585/1‑238 (MQ=255)

                                                                                                            tGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGaa                                                                                                                       >  1:1499544/1‑237 (MQ=255)

                                                                                                             gCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGaa                                                                                                                       >  2:616276/1‑236 (MQ=255)

                                                                                                                     aa‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCttt                                                                                                               >  1:877457/1‑236 (MQ=255)

                                                                                                                      a‑TGGTTGAAAA‑C‑CCT‑C‑CTCTCCTCGGGGACC‑GCCGCGGGCGTTATTACGG‑CCATGGTG‑CTGATTTTGATTTCCCCACC‑AGAAAAA‑C‑AGTAATCCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTctct                                                                                                            <  1:598518/239‑1 (MQ=255)

                                                                                                                      a‑TGGCTAAAAA‑C‑CCG‑T‑CTCTCCCCCGGGAGC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTCCCCCCA‑AAAAAAA‑C‑AGTAATCCACGGGCGGCGGAAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTctct                                                                                                            <  1:863433/239‑1 (MQ=255)

                                                                                                                                   acccT‑G‑CTCTCCTCCGGGCCC‑GCCCGGGGCGGTTTTCCTG‑CCATCGTG‑CTGAATTTGATTCCCCCCCC‑AGAAAAA‑C‑AGTATTTCCCGGGGGGGGGAAATAAAGTCCCCGCCGCAGTCGCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCAt                                                                                                  <  2:180971/237‑1 (MQ=255)

                                                                                                                                    aaccctctctccctcgGGCATC‑GCCGCGGGATTATTTCCTG‑CCTCCTTG‑CTGAATCTGATTTCCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAATATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCa                                                                                                   <  1:103582/228‑1 (MQ=255)

                                                                                                                                     ccT‑T‑CTCTCC‑CCGGGGTCGGCCGGGGGCGGTATTACGT‑GCATGGTG‑CTAAATCTATTTTCCCCACC‑AGAAAAA‑C‑ATTAATTCCCGGGCGGCGGAAAAATATTTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCg                                                                                                <  2:97833/239‑1 (MQ=255)

                                                                                                                                     acccgccccccccccggagcc‑gccggGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCCCC‑AAAAAAA‑C‑ATTAATTCACTGGGGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCAt                                                                                                  <  1:489324/219‑1 (MQ=255)

                                                                                                                                      aaccgccctccccccgggccgcccggGGGGGTTATTACTG‑CCCTGGTG‑CTGAACTTAATTTCCCCCCC‑AAAAAAA‑C‑ATTAATCCCTGGGGGGCGGAAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCAt                                                                                                  <  2:389318/217‑1 (MQ=255)

                                                                                                                                                 tccGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGt                                                                                      >  1:119797/1‑239 (MQ=255)

                                                                                                                                                 tccGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGt                                                                                      >  1:1000430/1‑239 (MQ=255)

                                                                                                                                                         c‑gcggcGGGCGGTATTACTG‑CCTTCGTG‑CTAAATCTGATTTTCCCACC‑AAAAAAA‑C‑AGTATTCCTCGGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCg                                                                              <  1:108269/239‑1 (MQ=255)

                                                                                                                                                           gccgcGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATc                                                                               >  2:1453296/1‑237 (MQ=255)

                                                                                                                                                            cggcgggcgtTATTACGC‑CCTTGGTG‑TGAAATCTGATTTCCCCACC‑AAAAAAA‑C‑ATTAATCCATGGGGGGCGGAAAGATAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCt                                                                            <  2:1372793/237‑1 (MQ=255)

                                                                                                                                                                 ggcggTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCCTTCCCCCCTTCAGGATTGCGCGTAAATCGTGCATAAATCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGCTCGCTTGcc                                                                        >  2:11110/1‑238 (MQ=255)

                                                                                                                                                                 ggcggTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGcc                                                                        >  2:22721/1‑238 (MQ=255)

                                                                                                                                                                            tG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTttct                                                              >  1:592886/1‑237 (MQ=255)

                                                                                                                                                                               ccATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACAtt                                                                                                       >  1:1516050/1‑194 (MQ=255)

                                                                                                                                                                                 catcgggtgaaaTCTGATTTCCCCACA‑AGAAAAA‑A‑ATTAATTCACGGGGGGCGGAAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGC‑‑AAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCaa                                                       <  1:545745/229‑1 (MQ=255)

                                                                                                                                                                                 aTCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTg                                                                                     >  1:319035/1‑210 (MQ=255)

                                                                                                                                                                                 aTCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCa                                                        >  2:485425/1‑239 (MQ=255)

                                                                                                                                                                                 aTCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCa                                                        >  1:1414714/1‑239 (MQ=255)

                                                                                                                                                                                   cGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCaa                                                       >  2:1110237/1‑238 (MQ=255)

                                                                                                                                                                                      g‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAAccc                                                   >  1:611204/1‑239 (MQ=255)

                                                                                                                                                                                           acttgatctgtCCCCCC‑AAAAAAA‑C‑ATAAATTTAGGGGCGGGGGAAAAAAAATCCCCGCCGCAGTCTCACCTCTTTAACCATGCCCGCCTTGAGGATTGCGCGTAAACCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAATCCCGCTg                                               <  2:1464966/229‑1 (MQ=255)

                                                                                                                                                                                           aaTTTGATTTCCCCCCC‑AGAAAAA‑C‑ATTATTGCGCTGCGGGCGGTAATATATTTACCGCCGCAGTTCCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTg                                               <  1:528562/239‑1 (MQ=255)

                                                                                                                                                                                           aaTCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGTAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTg                                               >  2:712539/1‑239 (MQ=255)

                                                                                                                                                                                           aaTCTGATTTTCCAACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCCAACCCGCTg                                               >  2:544679/1‑239 (MQ=255)

                                                                                                                                                                                             tttattttcCCCACA‑AGAAAAA‑C‑AGTAATTCCCGGGCGGCGGTAAGTAAATTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTggg                                             <  1:1034018/234‑1 (MQ=255)

                                                                                                                                                                                              tatgttttCCCCCCAAAAAAAA‑C‑ATAATTTCACGGGGGGCGGAAAAAAAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTggg                                             <  2:669728/235‑1 (MQ=255)

                                                                                                                                                                                                gATTTCCCCACA‑AAAAAAA‑C‑ATTAATTCCCTGGCGGCGGTAAAATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAg                                          <  1:1112506/239‑1 (MQ=255)

                                                                                                                                                                                                aaTTTCCCCCCA‑AAAAAAA‑C‑AGCAATTCGCGGGCGGCGGTAAAATAATTACCGCGGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAg                                          <  2:1012093/238‑1 (MQ=255)

                                                                                                                                                                                                 gttttCCCCCCAAAAAAAA‑C‑AATATTTCGCTGGCGGGGGTAATATATTTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAACTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAg                                          <  2:565874/238‑1 (MQ=255)

                                                                                                                                                                                                     tCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGGAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGa                                      >  2:675425/1‑238 (MQ=255)

                                                                                                                                                                                                         acc‑aGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACata                                 >  1:1391463/1‑239 (MQ=255)

                                                                                                                                                                                                           c‑aGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATc                               >  1:1305435/1‑239 (MQ=255)

                                                                                                                                                                                                                     ccagtaatcccggggcggcTGTAAGATATTTCCCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCAt                        <  1:367965/226‑1 (MQ=255)

                                                                                                                                                                                                                     aaattAATCCACGGGCTGCGGTAGGATAATTCCCGGCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCAt                        <  2:374632/235‑1 (MQ=255)

                                                                                                                                                                                                                                   gcggcgGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCCGAACATATCAGCGCATGGGTTTCCGAGAAt          >  2:1138944/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gcggcgGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAAt          >  1:190184/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gcggcgGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAAt          >  2:273397/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gcggTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGc       >  1:1200156/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gcggTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGc       >  1:569088/1‑239 (MQ=255)

                                                                                                                                                                                                                                         gaaaGATAGTGCCCCCCGCAGTTCCGCCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGa     <  2:1414714/236‑1 (MQ=255)

                                                                                                                                                                                                                                         gTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCg      >  1:1086914/1‑237 (MQ=255)

                                                                                                                                                                                                                                           aaGCTAATTACCGCCGCAGCCTCCCCTCTTTAAAAATTCGCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATAGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCAATCTTCTTCTGCAACCCCGCTGGGGAGCGGAACATCTCAGCGCACGGGTTTCCGAGACTAGCGCCTa  >  2:1246538/1‑239 (MQ=255)

                                                                                                                                                                                                                                           aaGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTATGCAAACCCGCTGGGGAGACGAACATATCAGCGCATGGGTTTTCGAGAATAGCGACTa  >  2:412494/1‑239 (MQ=255)

GTGCGTATCGTTTCTCGTGAGCCGCTGAACCGTCGGGCGATTCTGATTATCGCGCTGTCGCTGGCGGTTGGTCTGGGCGTGTCTCA‑GCAGCCGCTGATTTTGCAGTTTGCCCCTG‑AA‑TGGCTGAAAA‑A‑CCT‑G‑CTCTCCTCCGGGATC‑GCCGCGGGCGGTATTACTG‑CCATCGTG‑CTGAATCTGATTTTCCCACC‑AGAAAAA‑C‑AGTAATTCACTGGCGGCGGTAAGATAATTACCGCCGCAGTCTCACCTCTTTAACCATTCCCGCCTTGAGGATTGCGCGTAAATCGTGCATAACTCCCTTATGTGCATTTCACGGGATGGAAGACCATGAAATTTATTGGGAAGCTGCTTCTCTACATTCTCATCGCTCTGTTAGTGGCGATCGCTGGCCTCTATTTTCTTCTGCAAACCCGCTGGGGAGCAGAACATATCAGCGCATGGGTTTCCGAGAATAGCGACTA  >  NC_000913/3830129‑3830589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`