Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 12,484 C→A 20.9% Q108K (CAG→AAG)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091312,4840CA20.9% 309.1 / 21.4 138Q108K (CAG→AAG) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base C (73/34);  new base A (2/28);  total (75/63)
Fisher's exact test for biased strand distribution p-value = 1.16e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 141 total aligned reads displayed.

AAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCA  >  NC_000913/12254‑12721
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
aaGTCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAACGGCCAGAAAATg                                                                                                                                                                                                                                        >  1:629456/1‑239 (MQ=255)
      aTCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑cc                                                                                                                                                                                                                                  >  1:1348704/1‑238 (MQ=255)
      aTCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTAGAAGTTAAAGGCCAGAAAATGGCA‑cc                                                                                                                                                                                                                                  >  1:66924/1‑238 (MQ=255)
         cccccacCCTTTCTGTCATTCCCTACACCCAGGAGGGGAAAATTCTAGTTGGTCCGCCGGCTAAACGTGAGGAAGTGAC‑GAACCCGAAAAA‑CCCTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACAAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTAAAAATTATTGCTGCTGATACCGGCGACGC‑ATGGGTCGAATTTAAAGGCCAAAAAATGGCA‑ccgccg                                                                                                                                                                                                                              <  1:85313/236‑1 (MQ=255)
                           ttGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTAAAAATTATTGCTGCTGATAACGGCGACGC‑AGGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTg                                                                                                                                                                                                            <  2:224044/239‑1 (MQ=255)
                            tGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGTCAGAAAATGGCA‑CCGCCGCAGCTTTCTGCTGAAGTgt                                                                                                                                                                                                           >  1:1560257/1‑238 (MQ=255)
                            tGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGt                                                                                                                                                                                                             >  2:648455/1‑237 (MQ=255)
                            tGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCAATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGt                                                                                                                                                                                                             >  2:1422311/1‑237 (MQ=255)
                            tGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGCCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGCAGTACAGCGTGATGTTT‑CCATCATGCCGTTCACAATTATTGCTGCTGCTAACGGCGACGC‑ATGGGTCGAAGTTAAAGACAAGAAAATGGCA‑CCACCTCAGATTCCTGCTGAAGt                                                                                                                                                                                                             >  2:73000/1‑237 (MQ=255)
                              ccTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTg                                                                                                                                                                                                         >  1:1354802/1‑239 (MQ=255)
                              ccTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTg                                                                                                                                                                                                         >  2:639648/1‑239 (MQ=255)
                                tataCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGaa                                                                                                                                                                                                       >  2:1268943/1‑239 (MQ=255)
                                tataCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGaa                                                                                                                                                                                                       >  2:563970/1‑239 (MQ=255)
                                tataCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGaa                                                                                                                                                                                                       >  2:1049082/1‑239 (MQ=255)
                                  taCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGCAGTTAAAGGCAAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCCGaaca                                                                                                                                                                                                     >  1:999370/1‑237 (MQ=255)
                                   aCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGaaaaa                                                                                                                                                                                                    >  1:131517/1‑239 (MQ=255)
                                       aGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGCTTTCTGCTGAAGTGCTGCAAAAAATg                                                                                                                                                                                                >  1:985907/1‑239 (MQ=255)
                                        ggATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATga                                                                                                                                                                                               >  1:913282/1‑239 (MQ=255)
                                        ggATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATga                                                                                                                                                                                               >  2:1228484/1‑239 (MQ=255)
                                         gATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATgaa                                                                                                                                                                                              >  2:1561361/1‑239 (MQ=255)
                                         gATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATgaa                                                                                                                                                                                              >  1:510576/1‑239 (MQ=255)
                                             gtaaaaCTTTAGTTGGTCAGCGGGCTAAACGCCAGGCAG‑GACAGACCCCGCAAAA‑CCCTTTGTTTGCAATTAAACGCCTGA‑TTGGTCGCCCTTTCCAGG‑ACGAAGAAGTACAGCGGGATTTTT‑CCATCATGCCGTTAAAAATTATGGCTGCTGATACCGGCGACGC‑ATGGGTGGAAGTAAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATgaagaa                                                                                                                                                                                           <  1:1228484/235‑1 (MQ=255)
                                               gAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTctgct                                                                                                                                                                                                                  >  2:344694/1‑213 (MQ=255)
                                                   ctctAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGCCGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCCGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAAcc                                                                                                                                                                                       >  1:70107/1‑236 (MQ=255)
                                                             ttatcaggCGAACCGGCAGGGGGTGAA‑GAACCCGCAAAA‑CCTTCTGTTAGCGATAAAACGCCTGA‑TTGGTCGCTGGTTCAAGG‑GTGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTAAAAATTATTGCTGCTGATAACGGCGAGGT‑ATGGGTGGAAGTAAAAGGCAAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTAc                                                                                                                                                                           <  2:69249/232‑1 (MQ=255)
                                                             taaGCCGGATAAACGTAGGGCAGTGAG‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTAG‑TTGGTCGCCGTTTCCAGG‑AGAAAGAAGTACAGCGTGATGTTC‑CCATCATGCCGTTAAAAATTATTGCTGCTGATAACGGCGACGC‑AGGGGTCGAAGTAAAAGGCCAAAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTAc                                                                                                                                                                           <  1:1556379/236‑1 (MQ=255)
                                                                     ctacgcGTCTGCCAGTGAC‑CATCCCTCAAAA‑CCCTTTCTTGCTTTTTAAACGCCGGA‑GTGGTCGCCGCTACCAGG‑ACAAAGAAGTACAGCGTGATGTTC‑CCATCTGGCCTTTAAAATTTATTGCTGCTGTTAACGGCGACGA‑CTGGGTCGAAGTAAAAGGCCGGAAAATGCCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGa                                                                                                                                                                   <  1:1013187/233‑1 (MQ=255)
                                                                      tAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CAATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGCTTACCTGGGTGaa                                                                                                                                                                  >  1:1682375/1‑238 (MQ=255)
                                                                          gggcgggcgttGAC‑GAACCCCAAAAA‑CCCTCTTGTGGCAGTTAAACGCCTTT‑TTGTCCGGCGCTACCAGG‑ACGAAGAAGGACGGCGGGATGTTT‑CCATCAGCCCGTTCAAAATTATTGCGGCGGATAACGGCGACGC‑ATGGGGCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGt                                                                                                                                                             <  1:416073/229‑1 (MQ=255)
                                                                          cGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTTTTTTGGGGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGTTGATAACGGCGACGC‑ATGGGTCGAAGTAAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGt                                                                                                                                                             <  2:984943/239‑1 (MQ=255)
                                                                               ggCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTg                                                                                                                                                        >  1:1036851/1‑239 (MQ=255)
                                                                                  agagggacgaCCCACAAAAGCACTGTGTGTGCGATT‑AATGGCTGAGTTGGCGTCCGTTACAAGG‑AGGAGAAAGTGCAGCGAG‑TGTTTACCATCATGGCTTTCAAATTTATTGTTGTTGAAATGGGGGACGG‑AGGGGAGAAGGTTAAAGGAAAGAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTg                                                                                                                                                        <  1:151990/229‑1 (MQ=255)
                                                                                    tGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGGAACTGaa                                                                                                                                                      >  2:194490/1‑236 (MQ=255)
                                                                                     ggagaaccccGAAAAA‑CACTCTGTTGGCGATTAACCGCCTGA‑TTGGCCGCCGCTTCAGGG‑ACGAAGAAGTACGGCGGGATGTTT‑CCATCATGCGGTTAAAAATTATGGCTGCTGATAAGGGCGAGGC‑ATGGGTCGAAGTAAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGaa                                                                                                                                                                  <  1:664043/217‑1 (MQ=255)
                                                                                      aC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGt                                                                                                                                                 >  2:1132497/1‑239 (MQ=255)
                                                                                          aaCCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCGAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCTTTAAAAATTATTGCTGCTGAAAACGGGGACGC‑ATGGGTCGAAGTTAAAGGCAAGAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTAt                                                                                                                                              <  2:1665557/239‑1 (MQ=255)
                                                                                           ccccGAAAAA‑CTCTCTGTGTGCAATAAAACGCCGGG‑TTGGTCGCCGCTGCAGGG‑ACGAAGAAGTACAGCGTGGTGTTT‑CCATCATGCGGTTAAAAATTAGTGCTGCGGATAACGGCGACGC‑ATGGGTGGAAGTAAAAGGAAAAAAAATGCCC‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTAt                                                                                                                                              <  1:1414136/237‑1 (MQ=255)
                                                                                            cccGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATc                                                                                                                                             >  2:1256601/1‑238 (MQ=255)
                                                                                               gCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTg                                                                                                                                                        >  2:664043/1‑224 (MQ=255)
                                                                                               gCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTAAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCg                                                                                                                                         <  2:546056/239‑1 (MQ=255)
                                                                                                  aaa‑CACTCTGTTTGCGAGTAAACCCCTGA‑TGGGCCGCGGCTTCAAGG‑ACGAGGAAGTACAGGGTGATTTTT‑CAATCATGCCGTTCAAAATTATTGCGGCTGATAACGGCGACGC‑ATGGATCGAAGTTAAAGGCCAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTAc                                                                                                                                      <  1:1049082/239‑1 (MQ=255)
                                                                                                        ctgtttgtGCGATTAATCGCTCGT‑TTTGTCGCCGC‑TCCAGGTACGAAGAAGTACAGCTGGATGTTT‑CAATTCTGCGTTTAAAAATTATTGCGGCGGATGACGTGGACAT‑ATGGCACAAATTTAATGCCCA‑AAAATCGCACCCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGAACCGTTAACTGAAGCTGTTAGCACCGTACCGGCa                                                                                                                                 <  1:191522/232‑1 (MQ=255)
                                                                                                        ctctGTTTGCGATTAAACGCGTGT‑GTGGTCGCCTCTCCCAGG‑ACAAAGAAGTACAGGGTAATTTTT‑CAATCAGGCCGTAAAAAATTATTGCTGTTAATAACGGCGACGC‑TGGGGTCAAATTTAAAGCCAAAAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                                                                 <  2:385943/239‑1 (MQ=255)
                                                                                                        ctctGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTAc                                                                                                                                                                           >  2:760257/1‑197 (MQ=255)
                                                                                                        ctctGTTTGAAATAAACCCCCGGA‑TGGGTCGCCGCTTCCAGG‑ACAAGGAAGTCAAGTGTGATGTTC‑CCATATTGCCTTAAAAAATTATGGCGGTTGAAAACGGCAACGA‑TTGGGACAAAGTTAAAGACCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                                                                 <  2:1560257/239‑1 (MQ=255)
                                                                                                        ctctGTGTGCGATTAATCGCCTGA‑TGGGTCGCCGCTTCCAGA‑ACAAAAAAGTACCGCGTGTTGTTT‑CCACCATGCCGTTAAAAATTATTGCTGCTGATATGGGCGACGC‑ATGGTTCAAAGTTAAAGCCAAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                                                                 <  1:782699/239‑1 (MQ=255)
                                                                                                        cgctggtggcaaTTAAACGCTTGA‑TTGGTCGCCGTTTCCAGG‑AGGAAGAAGTACAGCGTGATGTTT‑CCACAAGGCTGTTAAAAATTATTGCTGCTGATAAGGGCGAGGA‑TTGGGTGGAAGTTAAAGGCCAAAAAATGCCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                                                                 <  1:1292184/228‑1 (MQ=255)
                                                                                                           tGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCAt                                                                                                                                >  2:191081/1‑237 (MQ=255)
                                                                                                           tGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCAt                                                                                                                                >  2:1700987/1‑237 (MQ=255)
                                                                                                           tGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCAt                                                                                                                                >  2:1337490/1‑237 (MQ=255)
                                                                                                                  gATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGATGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGTCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACAGTACCGGCATACTTTAACg                                                                                                                       >  1:1738418/1‑239 (MQ=255)
                                                                                                                            cTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCg                                                                                                             >  1:1574895/1‑239 (MQ=255)
                                                                                                                            cTGA‑TTGGTCCCCGTTTCCAGG‑ACGAAGAAGTACAGCTTGATGTTT‑CCAACATCCCGTACAAAATTATGGCTGCTAAAACCGGCGCCGC‑ATGGGTGAAAGTTAAAGGCCAGAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAATATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCg                                                                                                             <  2:726880/239‑1 (MQ=255)
                                                                                                                              gaatgggTCGCCTCTTCCGGG‑AAGAAAAAGTACAGCTTGATTTTT‑CCTTCAGGCCGTTCAAAATTATGGTTGCTGATAAGGGCGACGT‑ATGGGTCGAAGTTAAAGCCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGt                                                                                                            <  2:108373/234‑1 (MQ=255)
                                                                                                                                     gcgccgcTTCCAGA‑ACGAAGATGAACAGGGTAATGTTT‑CCTTCAGGCCGTTAAAAATTATTGCGGCGGATAACGGGGAGGC‑AGGGGTCGAATTAAAAGCCAAAAAAAGGGCC‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCa                                                                                                      <  1:639648/237‑1 (MQ=255)
                                                                                                                                         tgtttataagggacaggaagTACAGGGTGATTTTT‑CCATCATGCCGTTAAAAATTATTGCTGCGGATAACGGCGACGC‑ATGGGTCGAAGTAAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCa                                                                                                      <  1:470400/219‑1 (MQ=255)
                                                                                                                                              ccAGG‑AGAAAGAAGTACACAGTAATGTTC‑CCTCCATGCATTTTAAAATTATTGCTGCTGA‑AAAGGCCGCGCGTTGGGTCAAAGTAAAAGGCCAAAAAAGGCCC‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACg                                                                                            <  1:1194320/239‑1 (MQ=255)
                                                                                                                                                aGG‑AAGAAGAAATACAGCGGGATGTTT‑CCATCATGCCGTTAAAAATTATTGCGGCTGATACGGGCGCCGG‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCa                                                                                          <  1:888846/239‑1 (MQ=255)
                                                                                                                                                    aCGAAGAAGAACAGCTTGTTGTTT‑CCATCATCCCGTCAAAAATTATTGCTGCTAATACCGGCGCCGC‑AGGGGTCGAATTAAAAGGCCAGAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCa                                                                                          <  2:739909/236‑1 (MQ=255)
                                                                                                                                                     gaaaaaagcagagcgtttgtttt‑CCATCAGGCCGTAAAAAATTATTCCTGCTGAGACGGGCGCGGC‑AGGGGTCGAAGTTAAAGGCAAAAAATGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGc                                                                                       <  1:1132497/218‑1 (MQ=255)
                                                                                                                                                       aagaagTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGt                                                                                    >  2:1642388/1‑239 (MQ=255)
                                                                                                                                                        agaagTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTa                                                                                   >  2:1512769/1‑239 (MQ=255)
                                                                                                                                                                  cGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGCAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAACGACGCAGGCAGTATAGCTGGtgt                                                                         >  1:1732/1‑237 (MQ=255)
                                                                                                                                                                  cGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTgg                                                                            >  2:1380347/1‑236 (MQ=255)
                                                                                                                                                                    tGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACg                                                                                            >  2:470400/1‑218 (MQ=255)
                                                                                                                                                                    tGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTgg                                                                       >  2:1520470/1‑239 (MQ=255)
                                                                                                                                                                     tattttt‑CCATCATGCTGTAAAAATTTAGTGCTGAGGATAGCGGCGCCGC‑GGGGGGAAAAGTAAAAGGAAAAAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTgg                                                                       <  1:357597/234‑1 (MQ=255)
                                                                                                                                                                       tGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAg                                                                    >  2:282024/1‑239 (MQ=255)
                                                                                                                                                                       tGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAg                                                                    >  2:1616015/1‑239 (MQ=255)
                                                                                                                                                                       tGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAg                                                                    >  1:250381/1‑239 (MQ=255)
                                                                                                                                                                           tcccaacagcccgtaaaaaaTTATGGCTGCTGAAAACGGCGAGGA‑ATGGGTCAAAGTAAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTaa                                                                 <  1:1616015/223‑1 (MQ=255)
                                                                                                                                                                           t‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGACGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGCCCGTATCGCTGGGCTGGAAGTaac                                                                >  1:1450593/1‑238 (MQ=255)
                                                                                                                                                                               atcatGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACg                                                             >  1:1188404/1‑239 (MQ=255)
                                                                                                                                                                                 catGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTa                                                           >  1:1485220/1‑239 (MQ=255)
                                                                                                                                                                                  atGCCGTTCAAAATTATTGCTGCTGATACCGGCGAGGC‑ATGGTTCAAATTTAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTat                                                          <  2:259112/239‑1 (MQ=255)
                                                                                                                                                                                    gCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACg                                                                                                                       >  2:670743/1‑176 (MQ=255)
                                                                                                                                                                                        ttCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTAtcatca                                                     >  1:1350188/1‑238 (MQ=255)
                                                                                                                                                                                                  ttGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGCAGTAAAACGCATAATCAACGAaccgac                                           >  1:332571/1‑238 (MQ=255)
                                                                                                                                                                                                       gctgATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAg                                      >  1:1030650/1‑238 (MQ=255)
                                                                                                                                                                                                        ctgATAACGGCGACGC‑ATGGGTAGAAGTAAAAGGCCAGAAAAGGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGaa                                                                                                                                                      <  1:426006/125‑1 (MQ=255)
                                                                                                                                                                                                           aTAACGGCGACGA‑TTGGGTAGAAGTTAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTgcg                                 <  2:1647792/239‑1 (MQ=255)
                                                                                                                                                                                                             aaCGGCGACGA‑ATGGGTCGAAGTTAAAGGCAAGAAAATGCCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCt                               <  2:937742/239‑1 (MQ=255)
                                                                                                                                                                                                                  cGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCa                                                                                                                                            >  2:426006/1‑125 (MQ=255)
                                                                                                                                                                                                                  cGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCtt                          >  2:1618427/1‑239 (MQ=255)
                                                                                                                                                                                                                   gccGC‑ATGGGTCAAAGTTAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCtt                          <  2:1461351/236‑1 (MQ=255)
                                                                                                                                                                                                                   gACGC‑AGGGGTCGAATTAAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTa                         <  2:1464498/239‑1 (MQ=255)
                                                                                                                                                                                                                   gACGA‑AGGGGTGGAAGTTGAAGGCCAAAAAAGGGCT‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCtt                          <  2:985907/238‑1 (MQ=255)
                                                                                                                                                                                                                    gaggatggggTAGAAGTAAAAGGCAAAAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCtt                          <  2:1618208/231‑1 (MQ=255)
                                                                                                                                                                                                                    aCGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTAc                        >  2:1530578/1‑239 (MQ=255)
                                                                                                                                                                                                                     cGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGCGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCACTGTTCTGGAAGTAAAACGTATCACCAACGAACCGACCGCAGCTGCGCTGGCTTACg                       >  1:330023/1‑239 (MQ=255)
                                                                                                                                                                                                                         aggggTCAAAGTAAAAGGCCAAAAAAGGGCT‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGt                     <  1:1520470/236‑1 (MQ=255)
                                                                                                                                                                                                                            ggTCGAAGTAAAAGGCCAAAAAAGGGAA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTg                  <  1:282024/238‑1 (MQ=255)
                                                                                                                                                                                                                                 aggtaaaaGGCAAAAAAATGCCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACaa             <  1:1608542/233‑1 (MQ=255)
                                                                                                                                                                                                                                 aaGTTAAGGGACAAAAAGGGGAA‑CCGCCGCAGATTTCTGCTGAAGTGCGGAAAAAAATGAAGAAAACCGCGGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACa              <  2:477832/237‑1 (MQ=255)
                                                                                                                                                                                                                                       aaGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCGGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGc         >  2:345321/1‑236 (MQ=255)
                                                                                                                                                                                                                                       aaGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGCCCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGc         >  2:474768/1‑236 (MQ=255)
                                                                                                                                                                                                                                       aaGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGc         >  1:590939/1‑236 (MQ=255)
                                                                                                                                                                                                                                           ccAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGc   <  2:1036851/238‑1 (MQ=255)
                                                                                                                                                                                                                                           ccAAAAAATGGAA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGc   <  2:1662663/238‑1 (MQ=255)
                                                                                                                                                                                                                                            cAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCa  >  1:23185/1‑238 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
AAGGCGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGAC‑GAACCCGCAAAA‑CACTCTGTTTGCGATTAAACGCCTGA‑TTGGTCGCCGCTTCCAGG‑ACGAAGAAGTACAGCGTGATGTTT‑CCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGC‑ATGGGTCGAAGTTAAAGGCCAGAAAATGGCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCA  >  NC_000913/12254‑12721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑