BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,421,193	A→G	14.4%	S405G (AGT→GGT)	ulaA →	L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,421,193	0	A	G	14.4%	310.8 / 7.9	137	S405G (AGT→GGT)	ulaA	L‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand): ref base A (74/37); new base G (2/20); total (76/61)
Fisher's exact test for biased strand distribution p-value = 4.82e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGA  >  NC_000913/4420955‑4421429

cGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGa                                                                                                                                                                                                                                                <  1:1244794/239‑1 (MQ=255)

           gCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGAt                                                                                                                                                                                                                                      <  2:1310151/238‑1 (MQ=255)

             tttATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCCCCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATgg                                                                                                                                                                                                                                    <  2:514466/238‑1 (MQ=255)

             gttATGTGGGGCACCATCGGTCAGCTGATGGCGGTTGGCATCCTGGTCGCCTGCGTCTCCGCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAGTCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATggg                                                                                                                                                                                                                                   <  1:1392782/238‑1 (MQ=255)

               tATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GCAGAC‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TTTGGGCGGTGTAACTCC‑CCGG‑GATGAGTGCCTGGATGGGCa                                                                                                                                                                                                                                 >  1:380348/1‑239 (MQ=255)

               tATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGTGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCa                                                                                                                                                                                                                                 >  1:1523876/1‑239 (MQ=255)

               tATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCa                                                                                                                                                                                                                                 >  2:914033/1‑239 (MQ=255)

               tATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCa                                                                                                                                                                                                                                 >  2:152391/1‑239 (MQ=255)

               tATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCAGCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCa                                                                                                                                                                                                                                 <  1:732646/239‑1 (MQ=255)

                     gggCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCgg                                                                                                                                                                                                                          >  1:881703/1‑239 (MQ=255)

                      ggCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGGAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGa                                                                                                                                                                                                                         >  2:964641/1‑239 (MQ=255)

                      ggCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCgg                                                                                                                                                                                                                          >  2:685209/1‑238 (MQ=255)

                            aTCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑c                                                                                                                                                                                                                  <  1:635558/239‑1 (MQ=255)

                                gTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCATGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTGACCACATCGGCGGCTTGCGTGCGGCGAC‑GAAGAT‑TTGTCTGTGGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAATCTCA‑CCGG‑GATTGATGGCTGGGTGGGCAT‑GGCggtatgggcggt                                                                                                                                                                                                                >  1:1684036/1‑228 (MQ=255)

                                        aTTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGGTTCTGGCAcc                                                                                                                                                                                                      >  2:1372456/1‑239 (MQ=255)

                                        aTTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCAc                                                                                                                                                                                                       >  2:148094/1‑238 (MQ=255)

                                          tGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCCCTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGACATCTTT‑GGTTGCG‑TCTGGGCGGGGCAACTCA‑CCGG‑TATGGGTGCCTGGATGGGCAT‑GGCGGGCTGGG‑CGGTTCTGGccccc                                                                                                                                                                                                     >  1:1292157/1‑237 (MQ=255)

                                               ttGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑GATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACAGCCgatg                                                                                                                                                                                               >  1:252284/1‑239 (MQ=255)

                                                  gCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTTGAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGCCTGGG‑CGATTCTTGCACCGCCGatgatg                                                                                                                                                                                            >  1:776174/1‑239 (MQ=255)

                                                         ggTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGct                                                                                                                                                                                     >  2:287555/1‑239 (MQ=255)

                                                         ggTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCCTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGCTGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCCAGGct                                                                                                                                                                                     >  1:505145/1‑239 (MQ=255)

                                                            cGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑GATGAGTGCCTGGATGGGCAT‑GGCGGACCGGG‑CGGTTCTGGCACCGCCGGTGATGCAAGGcttct                                                                                                                                                                                  >  1:431667/1‑239 (MQ=255)

                                                                       cccggctCCTGTTTATTCCTGTCTTTCCCCGGATTTTTTCCCCGACCGCCCCCATCGGGGTGTCCGCTACCCACTGCGGGGGGGGGCGTGCGGCGCT‑GAATAT‑TTGTGTGGTGAGGGGGATGAT‑GAAATTTTTGGGTGGCG‑TGGGGGGGGTAAAACTCC‑CCGG‑TATGAGTGCCGGGAGGGGCGT‑GGCGGACGGGT‑CGTTCCTGGCTCCGCCGATGATGCAAGGCTTCTTCTCCATCGGt                                                                                                                                                                       <  1:1214361/233‑1 (MQ=255)

                                                                        cttgATCGTGATTATCCCGGGTTTTACCCCGATGTTCTTCTCTACCGCCCCCGTGGGGGTGTTGCATAACCCCTGGGGCGGGGGGGGGGGGGGGGG‑GAAATT‑TTGTGGGGTGAGGGGGAGGATGAAAATTTGT‑GGTTGGG‑TCGGGGGGGTAAACCTCA‑CCGG‑TAGGGGTGCCGGGGGGGGCAG‑GGCGGAGGGGC‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTa                                                                                                                                                                      <  1:543168/236‑1 (MQ=255)

                                                                        ctcGTTCGTGATTTTTCCTGGCTTTACCCCGATGTTTTTCTTAACCGCCCCCACGGGGGTGTTCGCTACCCCCGTGGGGGGGTGGCGGGCGGCGGT‑GAAGTT‑TTGTCGGGTGATGGGGATGATCGAAATTTTG‑GGTGGCG‑TGGGGGCGGGGAACCTCC‑CCGG‑TTGGGGTGCCGGGAGGGGCAT‑GGCGGATTGGT‑CGTTTTGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTa                                                                                                                                                                      <  1:1074695/239‑1 (MQ=255)

                                                                        ctcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTa                                                                                                                                                                      >  2:905710/1‑239 (MQ=255)

                                                                         tcGTTCCGGATTATGCCTGGCTTTATCCCGATGTTCTTCTCTAACCCCACCATGGGGTTGTTCGCTAACCCTTTCGGCGGCGGGCGTGGGGCGTT‑GAAGAT‑GTGTGGGGTGAGGGGGATGATCAAAATCTTT‑GGTTGCG‑TCGGGGCGGTGAAACTCC‑CGGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑AGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTAt                                                                                                                                                                     <  1:1430047/239‑1 (MQ=255)

                                                                         tcGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑GATGAGTGCCTGGATGGGCAT‑GGCGGGCTGGT‑CGATTCTGGCCCCGCCGCTGATGCAAGGCTTCTTCTCCATCGGTa                                                                                                                                                                      >  1:846968/1‑238 (MQ=255)

                                                                             tCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGGTGCAAGGCTTCTTCTCCATCGGTATCg                                                                                                                                                                   >  2:829576/1‑237 (MQ=255)

                                                                                 gATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTa                                                                                                                                                             >  2:586608/1‑239 (MQ=255)

                                                                                  attattCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCTGTATCGCCTTTAt                                                                                                                                                            >  2:1715540/1‑239 (MQ=255)

                                                                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGGT‑TTGTCTGGGGCTGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACGGGT‑CGATTCTGGCACCGCCGATGATGCCAGGCTTTTTTCCCAACGGTATCGCCTTTATGGcc                                                                                                                                                        >  1:629381/1‑238 (MQ=255)

                                                                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTtcttct                                                                                                                                                                               >  2:1658449/1‑215 (MQ=255)

                                                                                       tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTTTGGcc                                                                                                                                                        >  2:542864/1‑238 (MQ=255)

                                                                                            gCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCCTCTTCTCCATCGGTATCGCCTTTATGGCCGtca                                                                                                                                                    >  2:613114/1‑237 (MQ=255)

                                                                                                tATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTg                                                                                                                                              >  2:785854/1‑239 (MQ=255)

                                                                                                tATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTg                                                                                                                                              >  2:466551/1‑239 (MQ=255)

                                                                                                tATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTCGCGTTCGGCGCT‑GAAGAT‑TTGTCTGGAGCTGCGGATGATCGCAATCTTT‑GGCTGCG‑TCTGGGTGGTGAAACTCA‑CCGG‑TATGAGGGCCTGGATGGGCTT‑GGCGGCCTGGC‑CGCTTCTGGAACCGCCGATGATGCAAGGCTTCTTATACACCGGTATCGCCCTTATTCCCGTCATCCttt                                                                                                                                              >  1:312938/1‑238 (MQ=255)

                                                                                                 aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGGTCGAAATCTTT‑GGGTGCG‑TCTGGGGCGGGAAAATCA‑CCGG‑TATGAGTGGCTGGGTGGGCAA‑GGCGGACTGGT‑CGAATTTGGCGCCGCCGGTGGTGCAAGGACTCTTTTCCCTCGGTATAGCCTTTAAGGCCGTCCTCGtttt                                                                                                                                             >  1:804783/1‑237 (MQ=255)

                                                                                                 aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑AATGAGTGCCTGGCTGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCCAGGCTTCTTCTCCATCGGTATCGCCTTTCTGGCCGtcctacttgt                                                                                                                                             >  1:474778/1‑231 (MQ=255)

                                                                                                       aTGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGG‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGc                                                                                                                                       >  2:1474874/1‑239 (MQ=255)

                                                                                                        tGTTCTCCTCTAACCCCACACGGGGGGTTTCCGCAACCCCCTGGGGCGGTGGGGGGGGGGCGCA‑GAATTT‑TTGTTGGGGGAGGGGGTTGATGAAACTTTGT‑GGTTGCG‑TGGGGGGGGGAAACCTCC‑CCGT‑TTTGGGTGCCGGGAGGGGCAT‑GGCGGCCTGGT‑GGATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                      <  1:561508/239‑1 (MQ=255)

                                                                                                        ggTTCTTCTCTAGCGCCCCCCGGGGTGGGTTCGCGAACACCTGCGGCGGCTGGGGTGCGGCGCT‑GAAGTT‑TTGTGTGGTGATGGGGTTGATCGAAATTTTT‑GGTGGCT‑TCGGGGGGGTAAAACTCC‑CCGG‑TATGGGTGCGTGGAGGGGCAT‑GGCGGCCTGGT‑CGATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                      <  1:586608/238‑1 (MQ=255)

                                                                                                         gtcttgctgaaccGCCCCCAGGGGCGTTTGCGTAAACCACTTGGGCGTGTGGCGTGCGG‑GCTGGAAGTT‑TTGATGGGTGATGGGGAGAAGCAAATTCTGG‑GGTGGCG‑TGTGGGGGTTCAACCCCC‑CCGG‑TATGGGTGCATGGATGGGCAT‑GGCGGACTGGC‑GGTTTCTGGAACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                      <  1:710599/226‑1 (MQ=255)

                                                                                                          ttcttcCTTACCCCCACCTGCGGGGTTTTCCCTACCCCCTTGGGCGGCGGGGGGGCGGCGGT‑GAGGTG‑TGGTGTGGTGTGGGGGATGATGGAATTTTTG‑GGTTGCG‑TCTGGGGGGTAAAACTCC‑CGGG‑TATGGGTGCCTGGAGGGGCAT‑GGGGGACTGGT‑CGTTTCTGCCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCAc                                                                                                                                     <  1:466551/238‑1 (MQ=255)

                                                                                                            cttctCTACCCCCCCCATCGGCGTGTTCGCTAACCACTTGGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTTTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCGGGGCGGTGAACCTCA‑CCGG‑TAGGAGTGCCTGGAGGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTgg                                                                                                                                  <  2:546678/239‑1 (MQ=255)

                                                                                                                    aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGCAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑GATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGCATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATg                                                                                                                          >  2:1625446/1‑239 (MQ=255)

                                                                                                                    aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCCTCATTGTAATTTCCCTGGCTTTTATg                                                                                                                          >  1:827045/1‑239 (MQ=255)

                                                                                                                    aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCAACGGTATCGCCTTTATGGCCGGCATCATTGTAATTGCACTGGCTTTTATg                                                                                                                          >  2:1499633/1‑239 (MQ=255)

                                                                                                                    aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGG‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTTGCTTATATg                                                                                                                          >  2:977872/1‑239 (MQ=255)

                                                                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGttct                                                                                                                      >  2:462056/1‑238 (MQ=255)

                                                                                                                         accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGATATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGttct                                                                                                                      >  2:679712/1‑238 (MQ=255)

                                                                                                                          ccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAg                                                                                                                                                                                                                                               <  2:1396582/118‑1 (MQ=255)

                                                                                                                          ccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAg                                                                                                                                                                                                                                               >  1:1396582/1‑118 (MQ=255)

                                                                                                                          ccaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGttcttc                                                                                                                    >  2:1390729/1‑239 (MQ=255)

                                                                                                                           cagggggtgtttCGCTACCCTCTTGGTGGGTTGGGGTGGGGCGCT‑GAGGTT‑GTGTTTGGTGAGGGGGATAATCAAATTTTTG‑GGTGGCG‑TGGGGGCGGTGAACCCCC‑CCGG‑TGTGAGTGCCTGGTGGGCCGT‑GGCGGCCGGGT‑CTTTTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCg                                                                                                                   <  1:1702093/229‑1 (MQ=255)

                                                                                                                                gCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATTGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGGTGGGCAT‑GGCGGACTGGT‑CGATTCTCGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTTTAATTGCACTGGCTTATATGttttctcctgg                                                                                                               >  1:1239431/1‑229 (MQ=255)

                                                                                                                                           aaccgctgcgcgggttGGCGTGGTGCGCT‑GAGGTT‑TGGTGTGGGGAGGGGGAGGATAGAATTTTGT‑GTTGGGG‑TGGGGGGGGTGAAACTCA‑CGTG‑TATGGGTGCCTGGGGGGGCAG‑GGCGGACTGGG‑CGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcg                                                                                                   <  1:1476904/224‑1 (MQ=255)

                                                                                                                                             cctctcggggggcTGGCGTGCGG‑GGT‑GGAGATGTTGTGTGGTGAGGGGGATGATGGAATTTTTT‑GGTTGCG‑TGTGGGCGGTGAACCTCC‑CCGG‑TATGAGTGCTGGGATGGGCGT‑GGCGGACTGGT‑CGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcgcg                                                                                                 <  1:46106/229‑1 (MQ=255)

                                                                                                                                                           ggCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGGAGCAGATgac                                                                                   >  1:917791/1‑237 (MQ=255)

                                                                                                                                                           ggCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCa                                                                                   >  2:416553/1‑239 (MQ=255)

                                                                                                                                                                                    tgggggATGGGGATGATTGAAATCTTT‑GGTTGGG‑GCTGGGGGGGAAAACCCC‑CCGG‑AATGGGTGCCGGGAGGGGCAG‑GGCGGACGGGT‑CGATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                             <  2:776174/233‑1 (MQ=255)

                                                                                                                                                                                    gtggtggGGGGGATGACTGAAGTTTTT‑GGTGGCG‑TCTGGGGGGGAAAACTCC‑CGGT‑TATGAGTCCCGGGATGGGCTT‑GGCGGCCTGGT‑CTTTTCTGTCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                             <  1:508369/237‑1 (MQ=255)

                                                                                                                                                                                    cTGGTGTTGGGGTTGTTCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACCCA‑CGGG‑TATGAGTGCGTGGATGGGCAT‑GGCGGACTGGT‑CTATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                             <  2:1493228/238‑1 (MQ=255)

                                                                                                                                                                                    cTGGTAGTGGGGATGAGCGAAATTTTT‑GGTGGCG‑TCGGGGCGGTAAACCTCC‑CCGG‑TGTGAGT‑CCTGGGTGGGGCT‑GGGGGTCTGGTGCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                             <  2:597099/238‑1 (MQ=255)

                                                                                                                                                                                           tGGGGATGATCAAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTta                                                      <  2:215410/238‑1 (MQ=255)

                                                                                                                                                                                             gggATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTtaata                                                   >  1:1663739/1‑239 (MQ=255)

                                                                                                                                                                                             gggATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTtaata                                                   >  2:804650/1‑239 (MQ=255)

                                                                                                                                                                                              ggATGAGGGAAGTGTTG‑GGTGGCG‑TGGGGGGGGGAAACCTCA‑CGGG‑TAGGAGTGCCTGGATGGGCAG‑GGCGGACTGGT‑CGATCCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTtaata                                                   <  1:804650/238‑1 (MQ=255)

                                                                                                                                                                                                gtgaggaaagtttgt‑GGTTGCG‑GCTGGGCGGTAAAACTCC‑CGGG‑TATGGGGGCCGGGATGGGCAT‑GGGGGACTGGT‑GGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAg                                                 <  1:1500740/224‑1 (MQ=255)

                                                                                                                                                                                                atgatCGAAATCTTT‑GGTTGCG‑TCTGGGGGGTGAAACTCC‑CCGG‑TATGGGCGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAg                                                 <  2:827045/238‑1 (MQ=255)

                                                                                                                                                                                                aggatCAAAATCTTG‑GGTTGCG‑TTTGGGGGGTGAAACTCC‑CCGT‑TATGAGTGCGGGGAGGGGCGT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAg                                                 <  2:1065901/236‑1 (MQ=255)

                                                                                                                                                                                                 tgatCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATgg                                                                                                                                                          >  2:965226/1‑132 (MQ=255)

                                                                                                                                                                                                 tgatCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑GATGAGTGCCTGGATGGGCAT‑GGCGGACTGGC‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTACATGTTCTTCGCTGGCCGCGCGCTTCGCGCAGAAGAAGATGCAGAATAACAACTGGCAGAACAGTCTGCTTACTAATGa                                               >  2:1189171/1‑239 (MQ=255)

                                                                                                                                                                                                  gcatagaaattgt‑GGTTGCGTTCGGGGGGGTAAAACTCA‑CCGG‑TGGGTGGGCCGGGAGGGGCAT‑GCCGGCCTGCT‑CATTTCTGCCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAgg                                                <  1:152391/226‑1 (MQ=255)

                                                                                                                                                                                                  gatCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                              >  1:1366838/1‑239 (MQ=255)

                                                                                                                                                                                                  gatCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                              >  2:29951/1‑239 (MQ=255)

                                                                                                                                                                                                    tgaaatttgtt‑GGTTGCG‑TTTGGGCGG‑GAAACCCAGCGGG‑TTTGGGGGCCGGGA‑GGGCTTGGGCGAACTGGC‑GGATCCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                            <  1:977872/230‑1 (MQ=255)

                                                                                                                                                                                                        aaTTTTT‑GGTGGCG‑TCGGGGGGGTGAAACCCC‑CGGT‑TATGGGTGCCTGGGTGGGCAT‑GGCGGACTGGT‑CGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                            <  2:1082401/235‑1 (MQ=255)

                                                                                                                                                                                                         aTCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCCGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                       >  2:985969/1‑239 (MQ=255)

                                                                                                                                                                                                         aTCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAt                                       >  2:966533/1‑239 (MQ=255)

                                                                                                                                                                                                          ttttt‑GGTGGCG‑TCTGGGGGGTGAAACCCC‑CGGG‑TATGGGTGCCAGGATGGGCAT‑GGCGGACTGGT‑CGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGAtt                                      <  2:1247198/237‑1 (MQ=255)

                                                                                                                                                                                                           tttt‑gtttgCG‑TCGGGGCGGTGAAACTGA‑CGGG‑TATGAGTGCCTGGAGGGGCAG‑GGCGGACTGGT‑CGATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTa                                     <  2:1320675/238‑1 (MQ=255)

                                                                                                                                                                                                           tttt‑GGTGGCG‑TTGGGGCGGTAAACCCCC‑CCGG‑TTGGGGTGCCGGGATGGGCGG‑GGGGGACTGGT‑AGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTa                                     <  2:1523317/238‑1 (MQ=255)

                                                                                                                                                                                                                gtttGCG‑TCGGGGGGGTGAATCTCC‑CGGG‑TATGAGTGCCTGGAGGGGCAG‑GGCGGACTGTT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                                 <  2:380348/237‑1 (MQ=255)

                                                                                                                                                                                                                 gtggCG‑TCGGGGCGGTGAACCTCC‑CCGG‑TATGAGTGCCTGGAGGGGCGT‑GGCGGCTTGGT‑CGATCCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                                 <  2:1005080/235‑1 (MQ=255)

                                                                                                                                                                                                                  ttgggtggggggAGGTGACCCTCA‑CGGG‑TATGAGTGCCGGGAGGGGCGT‑GGGGGTCGGGG‑CGTTCCGGGCGCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                                 <  1:1625446/229‑1 (MQ=255)

                                                                                                                                                                                                                     ggctcgggggggTGAAACTCC‑CGGG‑TGTGTGTCCCTGGAGGGGTCG‑GGCGTATGGGT‑GGATTGTGGCTCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                             <  1:1150205/233‑1 (MQ=255)

                                                                                                                                                                                                                     cG‑TCGGGGGGGTGAACCCCC‑CGGT‑TATGAGTGCGGGTGTGGGCAT‑GGGGGACTGGT‑CGTTTGTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                             <  2:1292157/238‑1 (MQ=255)

                                                                                                                                                                                                                      g‑TCGGGGGGGGGAACCTCC‑CCGG‑TATGATGGCCTGGATGGGCAG‑GGGGGACTGGT‑GGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTCCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacg                           <  2:1355166/239‑1 (MQ=255)

                                                                                                                                                                                                                      ctggtGGGCGGTGAGACTCG‑CCGT‑GAGGGGTTTCTGTGTGGGAGG‑GGCGGACTGGT‑TGTTTCCGTCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                             <  1:1183759/234‑1 (MQ=255)

                                                                                                                                                                                                                         ggggggggTGTAACCCC‑CCGGTTATGAGTCCAGGGGGGGGCGT‑GGGGGACTGGT‑TGTTTGTGCCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgtacg                           <  1:1499633/236‑1 (MQ=255)

                                                                                                                                                                                                                                 tGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATg                                                                                                                          >  2:1518297/1‑134 (MQ=255)

                                                                                                                                                                                                                                 tGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGa                                  >  2:118599/1‑222 (MQ=255)

                                                                                                                                                                                                                                 tGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTg                    >  1:553352/1‑236 (MQ=255)

                                                                                                                                                                                                                                      cccA‑CCGG‑TTTGGTTGCTTGGATGGGCAT‑GGCGGGCTGGT‑GGTTTTTGGCTCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtg            <  1:1474874/237‑1 (MQ=255)

                                                                                                                                                                                                                                       cccgcggg‑TAGGAGTGCCGGGGTGTGCGG‑GGCGGCCTGTC‑CGATTTGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtg            <  1:1325440/233‑1 (MQ=255)

                                                                                                                                                                                                                                            ggg‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCa       <  2:1438043/238‑1 (MQ=255)

                                                                                                                                                                                                                                            cGG‑TATGAGTGCCGGGGGGGGCAG‑GGGGGACGGGC‑GGATTCGGGAACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCa       <  2:881703/239‑1 (MQ=255)

                                                                                                                                                                                                                                                  tGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGa  >  2:1339556/1‑239 (MQ=255)

CGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCT‑GAAGAT‑TTGTCTGGTGATGGGGATGATCGAAATCTTT‑GGTTGCG‑TCTGGGCGGTGAAACTCA‑CCGG‑TATGAGTGCCTGGATGGGCAT‑GGCGGACTGGT‑CGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGA  >  NC_000913/4420955‑4421429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`