Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	596,487	T→A	11.7%	N296K (AAT→AAA)	cusC →	copper/silver efflux system, outer membrane component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	596,487	0	T	A	11.7%	244.0 / 3.3	94	N296K (AAT→AAA)	cusC	copper/silver efflux system, outer membrane component
Reads supporting (aligned to +/- strand):  ref base T (30/50);  new base A (9/3);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 2.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGAAC  >  NC_000913/596250‑596715                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             tCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAat‑a                                                                                                                                                                                                                                            <  2:49612/239‑1 (MQ=255)    cgGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGGATGGCGTCGCAAATCTTATT‑TGAACGCCCGGGTACTTTTGAAA‑CTGAACAAGGGGTAATGGC‑GGCT‑ACTGCCAAA‑A‑ttt                                                                                                                                                                                                                                        >  1:1509567/1‑238 (MQ=255)       ggTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGgc                                                                                                                                                                                                                                      <  2:307684/238‑1 (MQ=255)             aGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCC‑AATGCCAAT‑A‑TTGGGGCTG‑Ca                                                                                                                                                                                                                               >  2:1046863/1‑238 (MQ=255)                 aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTTGTCGCAAATCTTATT‑TCATCGCCCCGATATTATGTAAG‑CTGAACACCCGCTAATAGC‑GGCC‑AATCACAAA‑A‑ttatggccgcaccgcc                                                                                                                                                                                                                           >  1:1535417/1‑225 (MQ=255)                 aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGTCCAT‑A‑TTTGCGCTG‑CCcgcgc                                                                                                                                                                                                                          >  2:1038569/1‑239 (MQ=255)                 aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CAcgcgc                                                                                                                                                                                                                          <  1:1206391/239‑1 (MQ=255)                 aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CAcgcgc                                                                                                                                                                                                                          <  2:196632/239‑1 (MQ=255)                 aGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGGCGTCGCAAATTTTATT‑TCAACGCCCGGGTATTATTGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑ATTGGGCTG‑CAcacgc                                                                                                                                                                                                                          >  2:1553822/1‑239 (MQ=255)                   tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTTTGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCCAT‑A‑TTTGCGCTG‑CCcccgcg                                                                                                                                                                                                                         >  2:1590949/1‑238 (MQ=255)                   tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGG‑GGCC‑AATACCCat‑aattgggcttccccacggg                                                                                                                                                                                                                          >  2:1039060/1‑221 (MQ=255)                   tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGGT‑AAAGACAAA‑A‑TTTGCGCtcgcagcgcgg                                                                                                                                                                                                                         >  2:1113611/1‑229 (MQ=255)                   tACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTTGGGCTG‑CAcgcgcg                                                                                                                                                                                                                         >  2:159069/1‑238 (MQ=255)                     cccGCAGCGACATCGCTAATCGTCAGGGGGAACTGGCGCACGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGTGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCCGCGGGATTGTCGTCGGAAATCTTATT‑GCCGCACACGGATATTCTTGAAG‑CTGAACACGCGTTAATGGC‑CGCT‑AATGCCAAT‑A‑TTGGCTCTT‑CACGCGCGGcg                                                                                                                                                                                                                      <  1:128774/239‑2 (MQ=255)                       cGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑Caataagatttgc                                                                                                                                                                                                                    <  1:1271954/239‑12 (MQ=255)                             gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCAtttttt                                                                                                                                                                                                                <  2:521109/237‑1 (MQ=255)                             gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑cc                                                                                                                                                                                                             <  2:1705121/239‑1 (MQ=255)                             gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑cc                                                                                                                                                                                                             <  1:557668/239‑1 (MQ=255)                             gACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGGCGCCAATCTTATT‑GCAGCGCCCGGATATTATTGAAG‑CTGAACAAGCGTTAATGGC‑GGGT‑AAATCCAAA‑ATTTGgcgcgccctcccgggcttttttt                                                                                                                                                                                                                 >  2:1140969/1‑219 (MQ=255)                              aCATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑cc                                                                                                                                                                                                             <  2:1410784/238‑1 (MQ=255)                              aCATCGCTAAACGTCAGGGGGAACTGGCGCAGACGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑cc                                                                                                                                                                                                             <  2:267169/238‑1 (MQ=255)                                    cTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCCAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACCCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTTGCGCTG‑CACGCGCGGCATTTTTT‑TCGTcctta                                                                                                                                                                                                      >  2:852877/1‑235 (MQ=255)                                       aaCGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGc                                                                                                                                                                                                  <  2:1014857/239‑1 (MQ=255)                                            cAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑c                                                                                                                                                                                           <  1:159069/239‑1 (MQ=255)                                            cAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑c                                                                                                                                                                                           <  2:528960/239‑1 (MQ=255)                                            cAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACGGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑c                                                                                                                                                                                           <  2:990162/239‑1 (MQ=255)                                             aGGGGGAGCTGGCGCACGCGAATAATGCATTGCAACTTTTGCCGGGAAGCCACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGATG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑c                                                                                                                                                                                           <  2:1273846/238‑1 (MQ=255)                                             aGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑TCAGCGCCCGGGTATTATGGAAG‑ATGGACACGCGGTAATGGC‑GGCT‑AAAGCCAAA‑A‑ATGGCGCCG‑GCCGCGCCGCAGTTTTT‑TCGTCTTT‑aaagccgaa                                                                                                                                                                                             >  1:1133929/1‑231 (MQ=255)                                                          agaggaaaataagGCATTGCAACTGTTGCGGGGAGGCGACGGCAAGCTGCCGAAAGCGCAGACAGTAAACAGCGACAGCCTGAAAAGCTTTAAATTCCCGGCGGGATTGTCGTCGCAAATCTTTTT‑GCGGCGCCCGGATATTAGGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AAGGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AACCCTG‑A‑CCAGCGAAATATCg                                                                                                                                                                              <  1:1297085/231‑1 (MQ=255)                                                             ggCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGGTTGTCGTCGCAAATCTTTTT‑TGAGCGCCCCGGTCTTATGGAAG‑CTGAACACGCGGTAATTGCGGGCA‑AATGCCCAT‑A‑TTGGGGCTG‑ACCCCCGGGCTTTTTTT‑CTGT‑TTT‑AAACCTC‑ACCCAGGGGAATATcacc                                                                                                                                                                            >  1:1030536/1‑235 (MQ=255)                                                                  aaaatGCATTGAAACTTTTGCTGGGAAGCTACGGCAAGGTGCCGAAAGCGCAGACATAAAACAGCAAAAGCCTACAATGCTTTAAATTACCGGCGGTATTGTCGTCGCAATTCTTATT‑GCTGCGCCCGGATATTATGGAAG‑CTGAACGCGCGTTAAGGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCTTTTTTT‑CCGTCTAT‑AAGCCTA‑A‑CCAGGGGAATATCGACCGCCAGt                                                                                                                                                                     <  1:1038569/237‑1 (MQ=255)                                                                   taatGCATTGCAACTGTGGCGGGGAAGCTACGGCAACCTGCCGCAAGCGCAGAAGGTAACCACCGACGGCCTGCAAAGCGTTAAATTACCGGGGGGATTGTCGCCGAAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGG‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AACCCTA‑A‑CCAGCGGAATATCGACCGCCAGTa                                                                                                                                                                    <  1:1093391/239‑1 (MQ=255)                                                                    aatGCATTGAAACTGTTGCTGGGAATCTAGGGAAGGCTGCCGAAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCTTTAATTTACCGGGGGGATTGTCGTCGAAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTAAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCGGCGGAATATCGACCGCCAGTAg                                                                                                                                                                   <  1:1046863/239‑1 (MQ=255)                                                                      tGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGGTATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCG‑TATGGCAATAA‑TTGGC‑CTG‑CCCCCGCGGCCTTTTTT‑CCCCTTAT‑TAACCTG‑A‑TCAGCCGGATATCGACCGccccttgcg                                                                                                                                                                 >  1:1703672/1‑232 (MQ=255)                                                                        cATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTTCTTGCAAATCTTTTT‑TCAACGCCCGGGTATTATTGAAG‑TTGAACACCCGGTAATGTC‑GGCT‑TCAGCCCAT‑T‑TTTGCCCTG‑CCCCCGCGGCCGTTTTT‑TCGTTTTT‑TAACCTG‑A‑CCCGCGGGAAATTGACCGCCacttgggg                                                                                                                                                                >  1:774313/1‑231 (MQ=255)                                                                        cATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑cc                                                                                                                                                                                                             <  2:1068784/196‑1 (MQ=255)                                                                        cATTGCAACTGTGGCGGGAAAGCTACGGCACGCTGCCGCAAGCGCAGACATAAACCAGCGACAGCCTGCAAAGCGTAAAATTACCGGCGGGATTGTCGTCGCAATTCTTATT‑GCACCCCCCGGATATTATGGAAG‑CTGAACACGCTTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CCCGCGCGGCTTTTTTT‑CCTTCTAT‑ACGCCTG‑A‑CCAGCGAAATATCGACCGCCAGTAGCg                                                                                                                                                                 <  1:1039060/237‑1 (MQ=255)                                                                            gaaacgtttGCGGGGAAGCACTGCCAACCTGCCGAACGCGAAGCAAATAAACAGCGACAGCCTGCAAAGCGTTAAATTCCCGGCGGGATTGTCGCGCCAAATCTTATT‑GCAGCACCCGGATATTATGGAGG‑CGGAACACGCGTTAATGGC‑GGAT‑AATGCAAAT‑A‑TTGGCGCTG‑CAGGCGCGGCATTTTTT‑CCGTCTAT‑AGGCCTG‑A‑CAGGCGGAATATCGACCGCCAGTAGCGATCTAt                                                                                                                                                           <  1:1417149/232‑1 (MQ=255)                                                                             aatcTGTGGCGGGGAAGCGACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGACG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGCCGCTG‑CCCGCGCGGCATTTTTC‑CCGTCTAT‑AAGCTTG‑C‑CCGGCGGAATATCGACCGCCAGTAGCGATCTAt                                                                                                                                                           <  1:1506657/235‑1 (MQ=255)                                                                                  gTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAA‑CTGAACACGCGTTAATGGC‑GGGT‑AAAGCCAAA‑A‑TTGGGGCCGCCACCGCCGCCATTTTTT‑CCGCCTTT‑AACCCT‑‑A‑CCACCGGGATATCGCCCCCCCGGAGGGGTCTTTCCTCat                                                                                                                                                     >  1:1439960/1‑239 (MQ=255)                                                                                        gggAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGGTATTATGGAAG‑CTGCACACGCGTTTATGGC‑GGCT‑CATGCCACA‑A‑TTTTCGCTG‑CCCCCGCGGCATTTTTT‑TCCTCTAT‑TAAGCTT‑A‑CCAACGGCATATTGCCCCCCCGTAGCGGTCTTTCGTCCttttttt                                                                                                                                               >  2:205676/1‑238 (MQ=255)                                                                                                 cgcgaacttGCCGGAAGGGAAGAAAAAAAACAGGGGCAGCCGAAAAAGCGTTAAATTCCGGGGGGGATTGTCGTGGCAATTCTTAGT‑GCAGCGCCGGAATATTATGGAAG‑CTGAACACGCTTTAAGGGC‑GGCT‑AATGCCAAT‑A‑TGGGCGCTG‑CCCGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCGGCGGAATATGGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCcagca                                                                                                                                      <  1:596002/231‑1 (MQ=255)                                                                                                                   gaagaaagaaAACAGCAAAAGCCGGAAAAGCGTAAAATTCCCGGCGGGATTGCCGCCGCAAATCTTATT‑GCAGCCCCCGGATATTATGAAAG‑CTGACCACGCGTTAATGGC‑GGCA‑AAGGCCAAT‑A‑TGGGGGCTG‑CACGGGCGGCATTTTTC‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAAtttt                                                                                                                    <  1:205676/236‑1 (MQ=255)                                                                                                                         aggaaacagcagcgggcgtcAAAGGTTTAAATTGCCGGTGGGATGGTCGTCGCAATGCTTATG‑GCACCGCCGGGATATTATGAAAC‑GGAAACACGCTTTAAGGGG‑GTTA‑AATGCCAAT‑A‑TTGGCGGTG‑AACGCCCGTCTTTTTTC‑CCGCCTAT‑AAGCTTG‑A‑CCAGCGGAAAACCGCCCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATAcc                                                                                                               <  1:467665/218‑1 (MQ=255)                                                                                                                         aGTAAAGAGCGACCGCCTGAAAAGGGTTAAATTACCGGGGGGATTGTGGTCGCAAATTTTATT‑GCAGCGCCCGGATATTAGGGAAG‑CTGAACCCGCTTTAAGGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTC‑CCGCCTAG‑AAGCCTG‑A‑CCAGCGGAATATGGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATAcc                                                                                                               <  1:1126553/237‑1 (MQ=255)                                                                                                                             aaCAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGGGCTG‑CACGCGCGGCCTTTTTT‑TCGGCTTT‑AAACCTG‑A‑CCAGCGGAAAATCGACCGCCCGGAACGATCTATCCTCATTATTTTAACCCCGCAGC‑GGGGAGTGGGATTTTTTATCCAAAAt                                                                                                         >  2:887939/1‑239 (MQ=255)                                                                                                                                   gACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCCAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAG‑A‑ATTGCGCTG‑CACGCGCGGCCTTTTTT‑CCGTCTCT‑AAGCCTG‑A‑CCAGCGGCATATCGACAGCCCGTAGCGGTCTTTTGTCACTATTTTACGCCGACCAC‑GGGGTGGGGGATTTTTTACCCAAACTTGAGAt                                                                                                   >  1:1296966/1‑239 (MQ=255)                                                                                                                                    aCAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGGCGCCAATCTTATT‑GCAGCGCCCGGGAATTATGGAAG‑CTGAACCCGCGTTAATGGA‑GGCC‑AATGCCCAA‑A‑TTTGCGCGG‑CCCCCGCGGCATTTTTT‑TCGTCTATAAAGCCT‑‑A‑CCCGCGGGATATTGACCGCCCGTAACGAACTCTCTTCACTTTTTAACACCACCAGC‑GGGAGGTTGGATTTTTTTCCCCACAtttggat                                                                                                   >  1:1297541/1‑233 (MQ=255)                                                                                                                                         ttgaaaaGCTTTAAATTACCGGCGGGATTGACGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CGGAACACGCGTTAATGGC‑GGCT‑AATGCCAAA‑A‑TGGGCGGGG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTTATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATcccc                                                                                               <  2:1476858/233‑1 (MQ=255)                                                                                                                                                     aaaTTACCGGCGGGTTTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGAAATATCGACCGCCAGTAGCG‑TCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGa                                                                                <  2:1182168/239‑1 (MQ=255)                                                                                                                                                      tagtACCGGGGGTGTTGGCCTCGCAAATCTGATG‑GCCGCGCCCGGATTTGAGTGATT‑CTATACTCGCGCTATTGGC‑GGCT‑GAGGCAAAA‑A‑TTGGCGCTC‑GCCGCGCGGCATTTTTC‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATAGCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGa                                                                                <  1:658633/236‑1 (MQ=255)                                                                                                                                                      aaTTACCGGCGGGATTGTCGTCGAAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGa                                                                                <  2:1439960/239‑1 (MQ=255)                                                                                                                                                            cggcggGATTGTCGACGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAGG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCAAAT‑A‑TTGGCGCTG‑CACGCGCGGCTTTTTTC‑CCGTCTAT‑AACCTTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAAc                                                                          <  1:878097/239‑1 (MQ=255)                                                                                                                                                            cggcggGATTGGCGACGCAAATTGTATT‑ACAGCGCCCGGATATTATGGAAC‑GGAAACACGGCTTAATGGC‑GGCA‑AATGCCAAT‑T‑GGGGCGCTG‑CGCGCGCGGCATTTTTT‑CCGATAAG‑ACGTCTG‑A‑ACAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCaa                                                                           <  1:882653/238‑1 (MQ=255)                                                                                                                                                             gggggggtttgcctgccaaaatttATTGGCAGCGCCCGGATATTA‑GGAAGCCTAAACCCGCTTTAATGGC‑GGTA‑AAGCCCAAT‑T‑TGGGGGTGG‑CACG‑GCGGGATTTTTTCCCGTTTAT‑AGGCCTG‑C‑CCAGCGAAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAAc                                                                          <  1:209287/217‑1 (MQ=255)                                                                                                                                                             ggcggGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGGTAAATCCCAAA‑A‑TT‑GCGCCG‑CCCCCGCGGCATTTTTT‑TCTGCTAT‑AAACCCT‑A‑CCAACGGGATATCGACCCCCCCGAGCGTCCTTTC‑TCATTTTTTTACCCCCGCAACGGGGAGGTGGATTTTTTTCCCCAAATTTAGGTTCCCCCTTTTTATTG‑CGGGCGCAAcc                                                                         >  1:1182168/1‑238 (MQ=255)                                                                                                                                                                 gtcttGTCGTCACAAATCTTCTG‑GCGGCGCACGGGGTGTGTGGATG‑CTCATCCCGTGTTAGTGGC‑GGCT‑AATCCAAAT‑A‑TGGGCGCTG‑CGCGCGGTGCGTTTTGT‑CCGTTTAT‑AAGACGA‑A‑CCAGCTAAACATCGACCGCCAGTAGCGATCGATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGATATCCCCATTTTTAATGCCGGACGCAACCAgg                                                                      <  1:1380559/235‑1 (MQ=255)                                                                                                                                                                 ggATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAgg                                                                      <  2:67448/238‑1 (MQ=255)                                                                                                                                                                 ggATTGGCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTAGGGAAG‑CTGAACACGCTTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAgg                                                                      <  2:675066/238‑1 (MQ=255)                                                                                                                                                                 gatttGTCGACGCAAATCTTATT‑GCGGCGCCCGGATTTTATGAAAG‑CTAAACACGCGTTAATGGC‑GGCT‑AAGCCCAAT‑A‑TTGGCGCTG‑CACGCGGGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑C‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAgg                                                                      <  1:1113611/235‑1 (MQ=255)                                                                                                                                                                                     aTT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATACCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑TCGTCTAT‑AAACCTG‑A‑CCAGCGGAATATCGACCGCCCCTAGCGATCTCACGTCATTATTTAACACCAGCAGC‑GGGGTGGGGAAATTTATACCCAACATTGAGCTCACAATCTTTAGTCACGGACGCAAACCGGCCAATATGGATATCGCCGgaa                                                 >  1:925745/1‑239 (MQ=255)                                                                                                                                                                                             cgcCCGGATATTATGGAGG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCCGTAGCGATCTAACGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAACTTTAGATCCCCATTTTTAATGCCGGACGACACCAGGCCAATCTGGATATCGCCGAAATTCGTca                                          >  1:470068/1‑239 (MQ=255)                                                                                                                                                                                             cgcCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCCTTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCCGCGGAATATCGACCGCCAGTAGCGATCTATCGTCCTTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCAAAAATTGAGAACCCCATTTTTAATGCCGGAAGCAAACAGGCCAAAATGGCAATCGGCGAAATTCTcc                                           >  1:486055/1‑238 (MQ=255)                                                                                                                                                                                             cgcCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGGCGCAACCAGGCCAATCTGGATATCGCCGAAATTCTCca                                          >  2:1541782/1‑239 (MQ=255)                                                                                                                                                                                             cgcCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCca                                          >  2:1136543/1‑239 (MQ=255)                                                                                                                                                                                             cgcCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCca                                          >  2:706034/1‑239 (MQ=255)                                                                                                                                                                                             cgcCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCC‑CATGCCAAG‑A‑TTGGCGCCG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCCGCGGAATATCGACCGCCCGTCGCGATCTATCGTTATTATTTAACCCCCGCAGC‑GGGGTGTGGGATTTTGTTCCCAACATTGGGCTCCCCCTTTTTAATGCCGGACGCAAACCAGCCAATCTGGATATCGCCTAAATTCGcc                                           >  1:1233906/1‑238 (MQ=255)                                                                                                                                                                                              gcCCGGATATTATGGAAG‑CTGAACACGCTTTAATGGC‑GGCT‑AATGCAAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGc                                            <  2:1030536/236‑1 (MQ=255)                                                                                                                                                                                              gcCCGGATATTAGGGAAG‑CAGACCACGCGTTAAGGGG‑GGAT‑AATGCAAAT‑A‑TTGGCGCTG‑CACGGGCGGCTTTTTTT‑CCGTCTAT‑AGCCCTG‑A‑CCGGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCcag                                         <  1:852877/239‑1 (MQ=255)                                                                                                                                                                                                       ttatGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCAAAT‑A‑TGGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGGGAAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGa                                                                                <  2:935462/191‑1 (MQ=255)                                                                                                                                                                                                       ttagGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCg                                <  2:1698334/239‑1 (MQ=255)                                                                                                                                                                                                        tagggAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AAGGCCAAT‑A‑TTGGCGCTG‑CCCGCGCGGCATTTTTC‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATACCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCgg                               <  2:915831/236‑1 (MQ=255)                                                                                                                                                                                                          tGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCggtg                             >  1:1384661/1‑239 (MQ=255)                                                                                                                                                                                                              aG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCCGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCCAACAGGCCAATCTGGATATCGCCGAAATTCGCCACCAGCAGTCGGGGGGGa                         >  1:310802/1‑239 (MQ=255)                                                                                                                                                                                                              aG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGa                         >  1:104261/1‑239 (MQ=255)                                                                                                                                                                                                              aG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGGCGGTGGTGa                         >  2:1128676/1‑239 (MQ=255)                                                                                                                                                                                                               g‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCACAAATTCCCCAGCAGCAGGCGGTGGTGaa                        >  1:1696806/1‑239 (MQ=255)                                                                                                                                                                                                               g‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑ATAGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAACCAT‑C‑CCAGCGGAATATCGACCGCCAGGAGCGATCTATCTTCATTATTTAACGCCAGCACC‑GGGATGTGGAATTTTATACACCATATTGGGATCCCCATTTTTAATACCGGTCGCAACCAGACCAATCTGGATATCGCCGAAATTCGTCACCCGCATGCGGTGGTGaa                        >  1:1300600/1‑239 (MQ=255)                                                                                                                                                                                                                 cTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAAt                       >  1:1510807/1‑239 (MQ=255)                                                                                                                                                                                                                  tGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAAtt                      <  2:1535417/239‑1 (MQ=255)                                                                                                                                                                                                                   gAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCCGCAACCGTCGGGGGGGGATTa                     >  1:915831/1‑239 (MQ=255)                                                                                                                                                                                                                    aaCACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATTGCCGAAATTCGCCAGCAGCAGTCGGCGGGGAATTAt                    >  2:1071863/1‑239 (MQ=255)                                                                                                                                                                                                                      cacgttttAATGGC‑GGTT‑AATCCCATT‑A‑TTGGCGCTG‑CCCGCGCGGCTTTTTTC‑CCGTTTAT‑AAGCCTG‑A‑CCAGCGGAATACCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGa                  <  1:706034/233‑1 (MQ=255)                                                                                                                                                                                                                      cacTCGTTAAGGGC‑GGCT‑AAGCCCAAT‑A‑TGGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAA‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGa                  <  1:1136543/239‑1 (MQ=255)                                                                                                                                                                                                                      cacGCGTTAAGGGC‑GGCA‑AATGCCAAT‑T‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGa                  <  2:1509567/239‑1 (MQ=255)                                                                                                                                                                                                                           tttAGTGGC‑GGCT‑AATGCCAAT‑A‑TTGGGGCTG‑CACGCGCGGCATTTTTT‑CCGTTTAT‑AAGCCTG‑A‑CCAGCGGAATATCGGCCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGa             <  2:451899/238‑1 (MQ=255)                                                                                                                                                                                                                              aaTGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGaaaa          >  1:1025672/1‑239 (MQ=255)                                                                                                                                                                                                                              aaTGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGGGAATTATGAACAGaaaa          >  2:354783/1‑239 (MQ=255)                                                                                                                                                                                                                                   c‑ggcT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAg     >  1:680730/1‑239 (MQ=255)                                                                                                                                                                                                                                      gcT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGa    >  1:703242/1‑238 (MQ=255)                                                                                                                                                                                                                                      gcT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAACAGTCGGTGGTGAATTATGAACAGAAAATCCAGa    >  2:686600/1‑238 (MQ=255)                                                                                                                                                                                                                                       cT‑AATGCGAAA‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTC‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGaa   <  1:1590949/238‑1 (MQ=255)                                                                                                                                                                                                                                       cT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGaa   <  2:703242/238‑1 (MQ=255)                                                                                                                                                                                                                                       cT‑AATGCAAAT‑A‑TGGGCGCTG‑CCCGCGCGGCATTTTTC‑CCGTCTAT‑AAGCCTG‑A‑CCGGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGaa   <  2:1297541/238‑1 (MQ=255)                                                                                                                                                                                                                                        ttaagcccAAT‑A‑TGGGCGCTG‑CAGCCGGGCAATTTTTT‑CCGTCTA‑‑AAACCTGAA‑CCAGCGGAATAACGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGaa   <  1:1071863/232‑1 (MQ=255)                                                                                                                                                                                                                                        t‑AATGCCAAT‑A‑TGGGCGCTG‑CACGCGCGGCATTTTTT‑CCTTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGAAc  <  2:1133929/238‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             TCGCGGGGTGATAGAAAGTACCCGCAGCGACATCGCTAAACGTCAGGGGGAACTGGCGCAGGCGAATAATGCATTGCAACTGTTGCTGGGAAGCTACGGCAAGCTGCCGCAAGCGCAGACAGTAAACAGCGACAGCCTGCAAAGCGTTAAATTACCGGCGGGATTGTCGTCGCAAATCTTATT‑GCAGCGCCCGGATATTATGGAAG‑CTGAACACGCGTTAATGGC‑GGCT‑AATGCCAAT‑A‑TTGGCGCTG‑CACGCGCGGCATTTTTT‑CCGTCTAT‑AAGCCTG‑A‑CCAGCGGAATATCGACCGCCAGTAGCGATCTATCGTCATTATTTAACGCCAGCAGC‑GGGATGTGGAATTTTATACCCAAAATTGAGATCCCCATTTTTAATGCCGGACGCAACCAGGCCAATCTGGATATCGCCGAAATTCGCCAGCAGCAGTCGGTGGTGAATTATGAACAGAAAATCCAGAAC  >  NC_000913/596250‑596715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑