BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	114,216	A→G	10.4%	E258G (GAG→GGG)	guaC →	GMP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	114,216	0	A	G	10.4%	410.9 / 6.3	125	E258G (GAG→GGG)	guaC	GMP reductase
Reads supporting (aligned to +/- strand): ref base A (3/107); new base G (13/2); total (16/109)
Fisher's exact test for biased strand distribution p-value = 3.65e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

ATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGC  >  NC_000913/113981‑114452

aTTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGaa                                                                                                                                                                                                                                                      <  2:869297/239‑1 (MQ=255)

    gCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGGCGCAGAG‑GTTAGGGGAAC‑ggg                                                                                                                                                                                                                                                 >  2:96679/1‑238 (MQ=255)

      cctggTTCTGTTTGTACACCTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTTGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCga                                                                                                                                                                                                                                               <  1:294171/236‑1 (MQ=255)

      ccAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCga                                                                                                                                                                                                                                               <  2:942132/239‑1 (MQ=255)

      ccAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCga                                                                                                                                                                                                                                               <  2:1104871/239‑1 (MQ=255)

      ccAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCga                                                                                                                                                                                                                                               <  2:1006424/239‑1 (MQ=255)

                 ttGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATg                                                                                                                                                                                                                                     <  2:585239/238‑1 (MQ=255)

                 ttGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATg                                                                                                                                                                                                                                     <  2:1038474/238‑1 (MQ=255)

                 ttGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATg                                                                                                                                                                                                                                     <  2:1017029/238‑1 (MQ=255)

                 ttGTACAACCCGCGGCAAACCAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATg                                                                                                                                                                                                                                     <  1:220167/238‑1 (MQ=255)

                 gtGTACGACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGGTGCTGCGCGCGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGGCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTGGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATg                                                                                                                                                                                                                                     <  1:197306/237‑1 (MQ=255)

                     aCAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCCACG‑TTGGGGGGAAC‑AGGGGGAGAATTATGCGGtg                                                                                                                                                                                                                                >  2:1021542/1‑238 (MQ=255)

                        aCTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCGTCG‑GTGGGGGGAAA‑GGCGGGAAAatttttatgttttt                                                                                                                                                                                                                             >  2:1088784/1‑228 (MQ=255)

                             cgTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCa                                                                                                                                                                                                                       <  2:1009861/239‑1 (MQ=255)

                              gTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCa                                                                                                                                                                                                                       <  2:1694711/238‑1 (MQ=255)

                              gTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCAGCG‑GTGGGGGGAAA‑GGGGGGAAAATTATGCTGTTCTAC‑gggac                                                                                                                                                                                                                      >  2:635616/1‑236 (MQ=255)

                               tCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCAt                                                                                                                                                                                                                      <  1:1026939/238‑1 (MQ=255)

                                cAAAACAGGCGGCGGTTATCCGCAGCGTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATg                                                                                                                                                                                                                     <  1:215426/238‑1 (MQ=255)

                                    aCAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTc                                                                                                                                                                                                                <  1:1023063/239‑1 (MQ=255)

                                    aCAGGCGGCGGTTATCCGCAACTTTCTGCGGTAAGCGAATGTGGCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTc                                                                                                                                                                                                                <  1:287885/239‑1 (MQ=255)

                                          gTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGt                                                                                                                                                                                                           <  1:1601271/238‑1 (MQ=255)

                                          gTCGGTTATCCGCAACTTTCTGCGGTAAACGAATGTGCCGATGATGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGGGAAAGACTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGt                                                                                                                                                                                                           <  2:66160/238‑1 (MQ=255)

                                                tctCCGCAGCTTTCTGCGGGAGTGGAATGTGACGATGCTGAGCACGGTCTGGGCGGAATGAGCGTCAGCGATGGTGGCGGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGAGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTGGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGAt                                                                                                                                                                                                   <  1:1512582/237‑1 (MQ=255)

                                                       aaCTTTCTGCGGTAAGCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGt                                                                                                                                                                                            <  2:616277/239‑1 (MQ=255)

                                                         cTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCCTCGTTTGGGGGGAAG‑GGGGGGAAATTT‑TGCTGTTTTTA‑CGCCTGAGGTCCCGGTCT‑GCGGGGAAAcacg                                                                                                                                                                                           >  2:1394132/1‑235 (MQ=255)

                                                            tCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTTGGGGGGAC‑CGGGAGAAAATTATGGTGTTTTAC‑CGCATGCGGTCCGGGTCT‑ggggtggaaaggcccct                                                                                                                                                                                       >  2:1672746/1‑223 (MQ=255)

                                                                          aggaggggaggctgcGCACGGTCTGGGCGGAATTGTCGGCAGCAATGGTGGCGGCACCCCGCCGGGCGATGTGGCGAAAGCCTTTGACTGGGGTGCCGATTTCGTCATGCTTGTCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCGAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCTCGTTGGC‑GGC‑GTTGCg                                                                                                                                                                        <  1:593611/228‑1 (MQ=255)

                                                                          aTGTGCCGATGCTGGGCACGGTCTGGGCGGAATGATGGTCGGCGGTGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCg                                                                                                                                                                        <  1:1240199/238‑1 (MQ=255)

                                                                                   tgctgcGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATcgcg                                                                                                                                                              <  2:15023/239‑1 (MQ=255)

                                                                                   tgctgcGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATcgcg                                                                                                                                                              <  2:1243207/239‑1 (MQ=255)

                                                                                     ctgcGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGt                                                                                                                                                                                                           <  2:94215/195‑1 (MQ=255)

                                                                                         gcACGGTCTGGGGGGAATGATGGTCAGCGATGGAGGCTGATCCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCAAGGTTGGCGGTATGCTGGCGGGGCACGAAGAGAGCGGC‑GGTCGCGTCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGACACGGTGGC‑GGC‑GTTGCGGAATATCGCgcagca                                                                                                                                                         <  1:336354/238‑1 (MQ=255)

                                                                                         gcACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCGCCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCgcagca                                                                                                                                                         <  1:1557175/238‑1 (MQ=255)

                                                                                         gcACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCgcagca                                                                                                                                                         <  2:129455/238‑1 (MQ=255)

                                                                                         gcACGGTCGGGGCGGAATGATCGTCAGCGATGGGGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCgcagca                                                                                                                                                         <  1:825165/238‑1 (MQ=255)

                                                                                         aagggacaggggCGGAATGATCGTCAGCGATGGTGGCTGCCCCACGCCGGGCGATGGGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGTGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGcagca‑g                                                                                                                                                       <  2:336149/230‑1 (MQ=255)

                                                                                           gcGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCGCCACGCCGGGGGATGTGGCGAAAGCTTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑a                                                                                                                                                     <  1:1712016/237‑1 (MQ=255)

                                                                                             ggTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGt                                                                                                                                                                                                           <  2:1427486/187‑1 (MQ=255)

                                                                                               tCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCgaga                                                                                                                                                                                                                                             <  2:12796/152‑1 (MQ=255)

                                                                                               tCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGgc‑ggc‑g                                                                                                                                                                             <  2:320317/212‑1 (MQ=255)

                                                                                                cTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTaa                                                                                                                                               <  2:1251175/239‑1 (MQ=255)

                                                                                                 tGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTaa                                                                                                                                               <  1:1354054/238‑1 (MQ=255)

                                                                                                 tGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTaa                                                                                                                                               <  1:1210768/238‑1 (MQ=255)

                                                                                                  gggCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTTGGGGGAAA‑GGGGGGGAATTTTTTATGTTTTTC‑GGCATGAGCTCCCGGGCT‑GCGGTGAAAAGTCCACTTGGCGGGG‑GTTGGGGAA‑ATTCCGCAGCA‑G‑AAGGGaaaa                                                                                                                                             >  2:1556149/1‑239 (MQ=255)

                                                                                                        aaTGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGgaga                                                                                                                                                                                                                                                       <  2:139360/134‑1 (MQ=255)

                                                                                                        aaTGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCCCATCG‑GTGAGGGGAACGGGGGGGAAAATTATGC‑GGTCTACAGGCAGCGGTTCCGGGCCT‑G‑GGTGGAAAGTCACCGTGGCGGGG‑GTTCCGGAAAATC‑CGCCGCA‑G‑AAGGGAAAAcgcgta                                                                                                                                       >  1:474837/1‑234 (MQ=255)

                                                                                                                           ggCTGGACCACGCCGGGCGATGTGGCGAAAGCGTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  2:246419/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCAGGCCGGCGGATGGGGCGAAAGCCTTTGGGGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  1:635616/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  2:885113/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  2:386406/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  1:751880/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  1:618406/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  1:235738/238‑1 (MQ=255)

                                                                                                                           ggCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGcc                                                                                                                    <  2:1244298/238‑1 (MQ=255)

                                                                                                                             cTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCg                                                                                                                  <  1:277305/238‑1 (MQ=255)

                                                                                                                             cTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCg                                                                                                                  <  1:80323/238‑1 (MQ=255)

                                                                                                                                     cGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGCGGGGGGCGCGG‑TTGGGAGGAAC‑GGGGGGAAATTT‑TTCTGTTTTTC‑GGCATGAGGCCCCAGGCTGGGGAGAAAAGGTCAC‑TTGGC‑GGC‑GGTGCGGGAGATACCGCCGCAGG‑AGGGAAAAACCGTGAA‑CTGCCGCCGCGG‑GGccccgctgggaaa                                                                                                         >  1:49588/1‑229 (MQ=255)

                                                                                                                                          ggCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTaa                                                                                                                                               <  2:326599/197‑1 (MQ=255)

                                                                                                                                            cGATGTGGCGAAAGGCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑Ccgcg                                                                                                  <  1:1341307/238‑1 (MQ=255)

                                                                                                                                            cGATGGGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑Ccgcg                                                                                                  <  2:470369/238‑1 (MQ=255)

                                                                                                                                               tgtgGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCgag                                                                                              <  1:1528087/239‑1 (MQ=255)

                                                                                                                                               tgtgGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCgag                                                                                              <  2:1033759/239‑1 (MQ=255)

                                                                                                                                                           cTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGggc                                                                                  <  2:1147914/238‑1 (MQ=255)

                                                                                                                                                                     ggTGCGGATGTCGTCGTGCTTGGGGGTATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGtt                                                                       <  1:596561/238‑1 (MQ=255)

                                                                                                                                                                     ggTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGtt                                                                       <  2:474837/238‑1 (MQ=255)

                                                                                                                                                                        gCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGc                                                                   <  2:1718689/239‑1 (MQ=255)

                                                                                                                                                                        gCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGc                                                                   <  2:178174/239‑1 (MQ=255)

                                                                                                                                                                        gCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGc                                                                   <  2:508858/239‑1 (MQ=255)

                                                                                                                                                                         ccGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGc                                                                   <  2:665851/238‑1 (MQ=255)

                                                                                                                                                                         ccGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGc                                                                   <  1:1390522/238‑1 (MQ=255)

                                                                                                                                                                         ccGATGTCGTCAGGCTTGGGGGCGTGCTGGCGGGCCACGAAGACAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCGGTTCTAC‑GGCATGAGCTCCGGGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGc                                                                   <  1:441311/238‑1 (MQ=255)

                                                                                                                                                                               tCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACa                                                            <  2:1621376/239‑1 (MQ=255)

                                                                                                                                                                               tCGTCATGCGTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGTTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACa                                                            <  2:150678/239‑1 (MQ=255)

                                                                                                                                                                                  tCATGCTTGGCGGCAGGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGa                                                                                                             <  2:61986/190‑1 (MQ=255)

                                                                                                                                                                                     tGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCTCCTTG‑GTGTGGGGAAA‑GGCGAGAAATTTTTGTCGTTCTAC‑CGCCTGGGACCCGCGGCT‑GCCGGGAAAAGTCACCGTGGC‑GGCGGTGGCGGAA‑ATTCCGCAGCC‑G‑GAGGGAAAAACGCTTAGCTGCCCGCGCGAGGGCCCGCTGGCAAAAA‑CCCCGCGGG‑ACTTTTTGGCGGGccgcggttcgcctttatatat                                                           >  1:17506/1‑217 (MQ=255)

                                                                                                                                                                                        ttGGCGGCATGCTGGCGGGCTACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTgg                                                    <  1:457913/238‑1 (MQ=255)

                                                                                                                                                                                              gcATGGTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTc                                              <  1:1672746/238‑1 (MQ=255)

                                                                                                                                                                                               cATGCTGGCGGGCCACGAAGAGAGCGGC‑GGGCGCATCG‑TTGAGGAGGAC‑GGCGGGGAATTTTTGGTGTTCTTC‑GGCATGCGGCCCGAGTCC‑TAGCGGAAAAATCCCGCTGGC‑GGG‑GGTGCGGGGAGTCGCCCCGCC‑G‑AAGACAAAACAGCTTAGCGCCCCGCGCGAGGGCCCGGTGGAAAAAACCCGCGCGAG‑A‑ATTTT‑GGCGGCCTG‑CGGTCAGCTTTGGCATAAGTTGGTGCTTCa                                             >  1:1004114/1‑238 (MQ=255)

                                                                                                                                                                                                aTGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCCTCG‑TTGGGGAGGAC‑GGGGAGAAAATTATGTTGTTCTTC‑GGCCTTAGCTCCGCGGCT‑GCGGTGAAACCTCACGGTGGG‑GGCGGTTCCGGAA‑ATACCGCCGCC‑GAAAGGAAAAACATTTAAACTCCCCCGCCGG‑GGCCGGGTTGAAAAAA‑CCCCCGGGG‑AAATTTTGGG‑GGCCTGCCGTTCGCCTCTTAAATACGTGGGGCCttac                                             >  1:945599/1‑236 (MQ=255)

                                                                                                                                                                                                aTGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACg                                           <  2:1369653/239‑1 (MQ=255)

                                                                                                                                                                                                aTGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACg                                           <  1:1696177/239‑1 (MQ=255)

                                                                                                                                                                                                 tGCTGGCGGGCCACGAAGAGAGCGGC‑GGGCGCATCG‑GTGGGGAGAAA‑GGGGGGAAAATTTTGCTGTTCTAC‑CGCCTGAGGCCCCGGGCT‑GCGGTGGAAAGGCACGGTGGG‑GGG‑GGTGCGGGATATCCCCCCGCC‑G‑GAGGGAAAAACGGTTAACTGCCCCCGCGC‑GGGCCGGGTGGAAAAA‑ACCCGCGAGAATATTTT‑GGCGGGCCG‑GGTTCCGGTTGTGTATACGTTTGGGGTGCACg                                           >  1:1235181/1‑238 (MQ=255)

                                                                                                                                                                                                     ggCGGGGCACGAAGAGAGCGGT‑GGTCGGATCG‑TTGAGGAGAAC‑GGCTAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAATGTCCCGTTGGC‑TGA‑TTTGCGGAATATCGCGTAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGa                                      <  1:933021/239‑1 (MQ=255)

                                                                                                                                                                                                     ggCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGa                                      <  2:1176129/239‑1 (MQ=255)

                                                                                                                                                                                                     ggCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGa                                      <  2:442029/239‑1 (MQ=255)

                                                                                                                                                                                                                 agagagCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGAcc                           <  2:714451/238‑1 (MQ=255)

                                                                                                                                                                                                                 agagagCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGAcc                           <  2:1537638/238‑1 (MQ=255)

                                                                                                                                                                                                                    gagCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAg                        <  1:1112575/238‑1 (MQ=255)

                                                                                                                                                                                                                      gcggc‑gGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAgcg                      <  2:1665834/238‑1 (MQ=255)

                                                                                                                                                                                                                      gcggc‑gGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAgcg                      <  2:1618145/238‑1 (MQ=255)

                                                                                                                                                                                                                       cggt‑ggTCGCAGCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGGGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCTGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAgcgcc                    <  1:192184/239‑2 (MQ=255)

                                                                                                                                                                                                                        ggc‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGcac                   <  2:17506/239‑1 (MQ=255)

                                                                                                                                                                                                                        ggc‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGcac                   <  2:796368/239‑1 (MQ=255)

                                                                                                                                                                                                                        ggc‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGca                    <  1:862677/238‑1 (MQ=255)

                                                                                                                                                                                                                        ggc‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGca                    <  1:1556149/238‑1 (MQ=255)

                                                                                                                                                                                                                          c‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGcacc                  <  2:1004114/238‑1 (MQ=255)

                                                                                                                                                                                                                          c‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGcacc                  <  1:1088784/238‑1 (MQ=255)

                                                                                                                                                                                                                          c‑ggTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGcacc                  <  2:1497161/238‑1 (MQ=255)

                                                                                                                                                                                                                                         gaggagAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCgtgt    >  2:1304984/1‑239 (MQ=255)

                                                                                                                                                                                                                                             agaAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCTGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGc  <  2:1259166/237‑1 (MQ=255)

ATTGGCCCAGGTTCTGTTTGTACAACTCGCGTCAAAACAGGCGTCGGTTATCCGCAACTTTCTGCGGTAATCGAATGTGCCGATGCTGCGCACGGTCTGGGCGGAATGATCGTCAGCGATGGTGGCTGCACCACGCCGGGCGATGTGGCGAAAGCCTTTGGCGGCGGTGCCGATTTCGTCATGCTTGGCGGCATGCTGGCGGGCCACGAAGAGAGCGGC‑GGTCGCATCG‑TTGAGGAGAAC‑GGCGAGAAATTTATGCTGTTCTAC‑GGCATGAGCTCCGAGTCT‑GCGATGAAACGTCACGTTGGC‑GGC‑GTTGCGGAATATCGCGCAGCA‑G‑AAGGTAAAACCGTTAAGCTGCCGCTGCGA‑GGCCCGGTTGAAAATA‑CCGCGCGAG‑ATATTTTGGGCGGCCTG‑CGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGC  >  NC_000913/113981‑114452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`