BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	954,395	G→A	10.9%	V24V (GTC→GTT)	focA ←	formate channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	954,395	0	G	A	10.9%	196.6 / 2.3	106	V24V (GTC→GTT)	focA	formate channel
Reads supporting (aligned to +/- strand): ref base G (31/59); new base A (5/7); total (36/70)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_000913/954158‑954595

cccACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAAGCTTAAGCGGA‑TGGTTCGTTGCTTTATA‑Ga                                                                                                                                                                                                         >  1:1452710/1‑239 (MQ=255)

   aCTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑Gaca                                                                                                                                                                                                       >  2:1325284/1‑238 (MQ=255)

           tAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCctct                                                                                                                                                                                              >  2:193112/1‑239 (MQ=255)

               aaaaaaaaacAACACGGGGGAAGTAAAGAGAGCGGCTCCGAAGACAACAAAAAGAAACAGCCCCAGAGAAAAGAAAATG‑CCGCCAACCAGTTTTGCCAGGCCGAAGGGTAGTGGGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAAGGCGATTG‑AG‑ATGAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGGGGT‑TTTTTCGTTGCTTTATA‑GACCCCCGCCTCTTCgg                                                                                                                                                                                          <  1:739325/230‑1 (MQ=255)

                 caacaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCCGc                                                                                                                                                                                         >  2:1638621/1‑238 (MQ=255)

                   acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCgggc                                                                                                                                                                                        >  2:789160/1‑235 (MQ=255)

                   acaaTCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑AAAG‑AAAGTCTTAAGCGGA‑TGTTTCCTTGCTTTATA‑GGCACCCGCCTCCTCGGCCa                                                                                                                                                                                       >  1:1160712/1‑238 (MQ=255)

                       tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCCG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTg                                                                                                                                                                                  >  2:1355827/1‑239 (MQ=255)

                       tCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAATCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GAAACCCGCCTCTTCGGCCACTTTg                                                                                                                                                                                  >  2:1194209/1‑239 (MQ=255)

                           cccGGTGAAAAAAAAGAGACCGGCCCCGCAAAAAACAAAAAGAAGCACCCCCGGAGAGAAGAAAATG‑CCGCCACCCAGTTTTCCCATGCAGAGGGGATTTGTGCCGGG‑GCCA‑GTGGTTGTTGTAATAT‑AGAAGAGGAAGGCGATTG‑AG‑ATAAAACCCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTGGTTGCTTTAAA‑GACCCCCGCCTCTTCGGCCACTTTGGCCa                                                                                                                                                                              <  1:1620216/237‑1 (MQ=255)

                             cGGTGGAAATAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGCCGAATGCGATTG‑AG‑ATGAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCAtt                                                                                                                                                                            <  2:1506077/239‑1 (MQ=255)

                                     gTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGGCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GAAACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    >  2:792278/1‑239 (MQ=255)

                                       aaaaagacgggtccgcaaaaaaacaCAAAAAACCAGCCCCAGGAAGAAGCAATGG‑CCCCCATCCAGTTTGGCTAGGCCGAAGGCTTGTGGGCCTGG‑GCCA‑GTGGTGGGTAGTAAAT‑AAAAGCCAAATGCGATTG‑AG‑AGGAAACCACCGGCGGTAA‑CCGCCAG‑ATAG‑AAATTCTGAAGGGGA‑TTTTTCGTTGTTTTAAA‑AACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    <  1:541617/216‑1 (MQ=255)

                                            gaTCGGCACCGCAGAAAACAAAAAGAATCACCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAGGGGTATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AAAAGACGAATGCGATTG‑AG‑ATAAAAACCCCGGCGGTAA‑GCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaa                                                                                                                                                              <  1:1328079/238‑1 (MQ=255)

                                             aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCCCTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            >  2:1617028/1‑239 (MQ=255)

                                             aTCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TAGCCAG‑ATAG‑AAAGTCTTAAGCGGG‑TGTTTCGTTTCTTTTTA‑GACACCCCCCCCTTCCGCCCCTTTGGGCCTTTCAGCCGGAAGTaaaa                                                                                                                                                            >  1:826810/1‑239 (MQ=255)

                                              gcGGCCCCGCAGACAAAAAAAAAAATACGCCCCAGAGAAAAGCAAAGG‑CCGCCAACCAGTTTTGCCAGGCCGAAGGG‑ATTTGGCCCGTGGCCA‑GGGGGTGCTGGAAAAT‑AAAAGACAAATGCGATTG‑AG‑AAAAAAACCCCGGGGGTAA‑TCGCCAG‑AAAG‑AAAGTCTAAAGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaaa                                                                                                                                                            <  1:399693/237‑1 (MQ=255)

                                               cGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTTGCCATTGCAGCCAGAAGTaaaaaa                                                                                                                                                          >  1:1114152/1‑237 (MQ=255)

                                                     cGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCCCTTTTGCCATTGCAGCAGGAAGTAAAAGATCaaaa                                                                                                                                                    >  2:379034/1‑239 (MQ=255)

                                                       cAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAgg                                                                                                                                                  >  1:1334059/1‑239 (MQ=255)

                                                            aacaCAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAATCTTAAGCGGG‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGGAAAAGATCAAAAAGGTTg                                                                                                                                              >  2:287540/1‑238 (MQ=255)

                                                                 aaaGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCACCCGTTTTGGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTAATAT‑AGAAGACGAAGGCGATGG‑AG‑ATAAAACCACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCt                                                                                                                                        <  2:640238/239‑1 (MQ=255)

                                                                 aaaGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATAAAAACACCGGGGGTAA‑TCGCAAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTAAA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCt                                                                                                                                        <  2:180771/239‑1 (MQ=255)

                                                                   agacacaccccaAGAGAGAGGCAAGGC‑CCGCAAACCGTTTTTCCCATCCGGAAGGCCTTTTTGCTTGT‑GCAA‑GTGGTGGCGGTAATAT‑AAAAACCGAATGCGATTG‑AG‑AGAAAAACCCCGGCGGTAA‑TCCCAAG‑ATAG‑AAATTTTTAAGGGGT‑TTTTTCTTTGTTTTATA‑AACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCttt                                                                                                                                      <  1:878063/231‑1 (MQ=255)

                                                                   aacgtcgccacgagaggaagacAAATC‑CCGCGAACCTGGTTGGCAATGCAGAAGGACATGGGGCAGGC‑GCTG‑GTGGGTGCTGTGATAT‑AGAAGACGACTGCTATCG‑AA‑ATAAAAACCCCGGCGTAAA‑TCGCCAG‑AAAG‑AAAGTCTTAAGCGGA‑TTTTTCTTTGTTTTAAA‑AACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGCGTTGTCAGCttt                                                                                                                                      <  1:1236827/218‑1 (MQ=255)

                                                                   aGAACCAGCCCAAGAGAGAAGCAAATG‑CCGCCAACCATTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACCCCGGCGGTAA‑CCGCCAG‑ATAA‑AAAGTCTTAAGCGGA‑TTTTTCTTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCttt                                                                                                                                      <  2:1199886/239‑1 (MQ=255)

                                                                           ccccAGAGAAAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCAGAGGGGCATTGGGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACCCCGGCGGTAA‑CCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTAAA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    <  1:12598/201‑1 (MQ=255)

                                                                            cccGGAAAGAAAAAAAGG‑CGGCTACCCTGTTTTGCCAGGCCGAGGGGCGTGGGCGATGA‑ACGA‑GTGGTTGTGGTGAAAA‑GAAAGCAGAATCGGATTG‑AG‑AAGAAAGCCCGGGGGGTAA‑TCCCCAG‑ATAA‑AATGTATTAAGCGGA‑TGTTTTTTTGTTTTAAA‑AACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACt                                                                                                                                <  1:193112/236‑1 (MQ=255)

                                                                               agagagAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TACGCAG‑ATAG‑AAAGTCCTTAGCGGA‑TGTTTCCTTGCTTTATC‑AACACCCGCCTCGTCGGCCACTTTGGCCCTTGCAGCAGGCAGACAAAAGTTAAAAGGGTTGTCAGCTTTCTAactcactc                                                                                                                           >  1:1633141/1‑238 (MQ=255)

                                                                                 gaggaAGCAAAGG‑CCGCCAACCAGTTTTGCCATGCGGAAGGGCATTGTGCCTGT‑GCCA‑GGGGTTGCTGTGATAT‑AAAAGACGAATGCGATTG‑AG‑ATAAAACCCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActctc                                                                                                                         <  2:737959/235‑1 (MQ=255)

                                                                                  ggaaaagaaaTGCCCGCAAACCGTTTTTGCCAGGCGGAAGGGCATTGTTCGTGG‑GCAA‑GTGGTTGTTGTGATAA‑AGAAGAAGAATGCGA‑TG‑AGAATAAAACCCCCGGCGGTAA‑TCGCAAG‑ATAG‑AAATTCTAAAGCGGA‑GTTTTCGTTGCTTTAAA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActc                                                                                                                           <  1:1089205/229‑1 (MQ=255)

                                                                                     aaGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGC‑TGTTTCGTTGCTTTATA‑AACACCCGGCTCTTCGGCCACTTTGGCCATTGCACGAGGAAGGAAAAGATCAAAAGGGTTGTCTGCTTTCACACCAACTCTCTCttt                                                                                                                    >  1:920741/1‑239 (MQ=255)

                                                                                     aaGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGa                                                                                                                                                          >  2:12598/1‑201 (MQ=255)

                                                                                        cAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGGCTTAAGCGGA‑AGTTTAGTTGCTTTATA‑GACACCCGCCTCTTCCGCCACTTTGGGCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAACTCTCTCTttatt                                                                                                                 >  2:284585/1‑239 (MQ=255)

                                                                                         aaaTG‑CCGCCAACCAGTTTTGCCAGGCGGAGGGGCATTGTGCCGGG‑TCCA‑GGGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATAAAACCCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTAAGGCGTA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTttat                                                                                                                  <  1:1299285/237‑1 (MQ=255)

                                                                                                 gcaacccATTTTTGCCATGCCGAAGGGAATTGTGCCTGT‑GCCG‑GTGGTGGCTGTGATAT‑AGAAGACGAAGGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGc                                                                                                         <  2:735529/234‑1 (MQ=255)

                                                                                                    aaacttttttGACATGCAGAAGGGTATTGTGCCGGT‑GCCA‑GGGGTTGGTGTAAAAA‑AAAAAAAGAAGGAGAGTG‑AG‑ATAAAACCCCCGGGTGTAA‑CCGCCAG‑ATAA‑AAATTTTTAAGCGGT‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  1:1617028/233‑1 (MQ=255)

                                                                                                    aaCCATTTTTGCCAGGCGGAAGGGCTTTGTGCCTGG‑GCCA‑GTGGTTGCTGTGATAT‑AAAAGACAAATGCGATTG‑AG‑ATAAAAACACCGGCGGTAA‑TCGCCAA‑TAAG‑AAAGTCTTACGCGGA‑TTTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGcgac                                                                                                      <  2:240088/239‑1 (MQ=255)

                                                                                                          ttttGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTTTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCCTCGGCCACCTTTGCCATTGCAGAAGGAAGTAAAAGATCACAAGGGTTGTTAGCTTTcac                                                                                                                                   >  2:1441567/1‑204 (MQ=255)

                                                                                                           ttggCCAGGCAGAGGGGAATTGTGCCGGT‑GCAA‑GTGGTGGCTGTGATAA‑AGAAGACGAAGGCAATTG‑AG‑ATAAAAACCCCGGCGGTAA‑CCGCCAG‑AAAA‑AAAGTCTTAAGCGGA‑TTTTTCTTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGAt                                                                                                 <  2:821074/234‑1 (MQ=255)

                                                                                                           gtgccaatgcggaggggattggggcctgt‑gccA‑GTGGTGGCTGGGATAA‑AGAAGACAAATGCGATGG‑AG‑AGAAAACCCCCGGGGGTAA‑CCGCCAG‑AAAG‑AAAGTTTAAAGCGGA‑TTTTTCGTTTTTTTATA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGAt                                                                                                 <  1:1415151/214‑1 (MQ=255)

                                                                                                            ttGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAA‑ATAG‑AAAAGCTTAAGCGGA‑TGTTTCGTTGCTTTATT‑GACACCCGCCTCTTCGGCCACTTTGGCCCTTGCAGCAGGAAGGAAAAGATCAAAAGGGTTGTCAGCTTTCCCCCTAACTCTCCCTTTATTAAGTCGGCGACGAGATACt                                                                                              >  1:882305/1‑239 (MQ=255)

                                                                                                               ccATGCCGAAGGGCATTGTGCCGGT‑GCCA‑GGGGTTGCTGTGATAA‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTAAAGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTaa                                                                                            <  2:826810/238‑1 (MQ=255)

                                                                                                                ttgcccaaaGGCCATTGTCCCTG‑‑GCCATTTGTTTGTTGTGATAT‑AAAAGACAAGTGCGTTAG‑AG‑ATGAAAACACCGGGGATAA‑CCGCCAG‑ATAG‑AAAGTTTTAATGTGA‑TGTTTCTTTGTTTTAAA‑GCAACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  2:1287763/232‑1 (MQ=255)

                                                                                                                cATGCGGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATAAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTAAAGCGGA‑TGTTTCGTTGTTTTATA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACa                                                                                          <  2:1114152/239‑1 (MQ=255)

                                                                                                                    ccGAAGGGCATTGTGCCTGT‑GCAA‑GTGGTTGCTGGGATAT‑AGAAGACGAAGGCGATTG‑AG‑ATGAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAGGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAg                                                                                       <  2:746397/238‑1 (MQ=255)

                                                                                                                            ctttGTGCCTGT‑CCCG‑GTGGTTGCTGTGATTG‑AAAAGCCGAATGCGATTG‑AG‑ATGAAAACCCTGGGGGTAA‑GCGCAAG‑ATAA‑AAATTCTTAAGGGGT‑TTTTTCGTTGTTTAAAA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAgg                                                                                                                                                                    <  1:1686316/151‑1 (MQ=255)

                                                                                                                            cATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGa                                                                              >  2:1663671/1‑239 (MQ=255)

                                                                                                                                gtggcttgcgccA‑GTGGTGGCTG‑GAAATGAGAAGACGGAGGCGTTGA‑AG‑AGAAAAACCCCGGCGGGAA‑TCGCCAG‑ATAG‑AAATTCTTAGGCGGG‑TGTTTCGTTTTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATga                                                                           <  1:287540/230‑1 (MQ=255)

                                                                                                                                 gttgccgtggcgatggtttGCTGTGATAT‑AGAAGA‑GAAAGGGGTTGAAG‑AGAAAACCTCGGGCGTTAA‑TCGCAAT‑AAAA‑AAATCCTTAAGCGGA‑TGTTTGGTTTTTTAATA‑GAGCTCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATga                                                                           <  1:859454/222‑1 (MQ=255)

                                                                                                                                 gggaaatgtgtaaaagagaaaggtgATAA‑AGAAGACGAATGGGATTG‑AG‑ATAAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTGAAGCGGA‑TGTTTCTTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTCATTAAGTCGGCTACGAGATACTAACAAAGCATTATAGATga                                                                           <  2:1305198/217‑1 (MQ=255)

                                                                                                                                    ctgt‑gccA‑GTGTTTGCTGTAATAT‑AAAAGACAAATGCGATTG‑AG‑ATGAAACCACCGGCGTTAA‑TCGCAAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGaaa                                                                       <  2:191841/238‑1 (MQ=255)

                                                                                                                                      gt‑gccA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGACGCAGGAAGTAAAAGa                                                                                                                                                          >  2:1686316/1‑153 (MQ=255)

                                                                                                                                         gccA‑GTGGTTGTTGTGAAAT‑AGAAGACGAAGGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGat                                                                  <  2:1160712/239‑1 (MQ=255)

                                                                                                                                          ccA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAACGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTTACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAACAATATAAAAGAGAAATTtata                                                                 >  1:107315/1‑239 (MQ=255)

                                                                                                                                           ctgttgttggCTGTGATAT‑AGAAGGGGAATGCGATTG‑AG‑AGGAACACACCGCTGGTAA‑TGGCCAG‑TTAG‑AATTTCTTAGGTGGA‑TGTTTCGTTGCTTAATA‑GACTCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  1:1588772/230‑1 (MQ=255)

                                                                                                                                           ccatgggcgcgggtgAGAG‑AGAAGAAGAAGGGGGTTG‑AG‑AAAAAAACACCGGCGGTAG‑TCGCCAG‑AGAG‑AATGTATGAAGCGGT‑TGTGTTGGTGTTTAATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  1:411127/227‑1 (MQ=255)

                                                                                                                                           cA‑GTGTTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTTTTAAGCGGA‑TGTTTCGTTGTTTTATA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:647106/238‑1 (MQ=255)

                                                                                                                                           cA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCAATTG‑AG‑ATGAAACCACCGGCGGTAA‑CCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:1452710/238‑1 (MQ=255)

                                                                                                                                           cA‑GTGGTTGCTGTGATAT‑AGAAGACGAAGGCGATTG‑AG‑ATAAAAACCCCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:1680382/238‑1 (MQ=255)

                                                                                                                                           cA‑GTGGTTGCGGGGATAT‑AAAAGACAAATGCGATGG‑AG‑AGAAAAACCCGGGCGGTAA‑CCGCCAG‑ATAG‑AAAGTCTAAAGCGGA‑TGTTTCGTTGTTTTAAA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  2:868158/238‑1 (MQ=255)

                                                                                                                                           cA‑GGGGTTGTTGTGATAA‑AAAAGACGAAGGCGATTG‑AA‑ATAAAAACACCGGCGTTAA‑TCGCAAG‑ATAG‑AAATTCTTAAGGGGT‑TGTTTCGTGGCTTAATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGatat                                                                <  1:15985/239‑1 (MQ=255)

                                                                                                                                            gagggttttatgtgATGT‑AAAAGACGAATGCGATTG‑AG‑ATAAAAACCCCGGCGTAAA‑CCGCCAG‑AAGG‑AAAGTCTTAAGCGAG‑TGTTTCGTTGCTTTATA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGata                                                                 <  1:789160/228‑1 (MQ=255)

                                                                                                                                               tGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑AGTTTCGTTGCTTTATA‑GACACCCGCCTCTTTGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTTTTAAGACGGCGACGAGGAACTAAAAAAGCATTATAGATGAGAAATTGAtttaaa                                                             >  1:1322375/1‑237 (MQ=255)

                                                                                                                                                     ctggtatat‑AAAAGACAAGTGCGATGA‑AG‑ATAAAAACCCGGGGGTTAA‑TCGCCAT‑AGAA‑AAATTTTAAGGCGGT‑TGTTTCTTTTTTTTATA‑GACCACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCatat                                                       <  1:1700994/234‑1 (MQ=255)

                                                                                                                                                       tttgaaaaaaaagacaagggCGATTG‑AG‑ATGAAAACCCCGGGGGTAA‑TGCCTAT‑ATAG‑AAAGTCGAAAGCTGA‑TGTTCCGTTGCTTTATA‑TGCTCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCatat                                                       <  2:1633141/219‑1 (MQ=255)

                                                                                                                                                           tat‑aAAAGACGAAGGCGATTG‑AA‑ATAAAAACCCCGGCGGTAA‑CCGACAG‑ATAG‑AAAGTTTAAGGCGGA‑TTTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:1663671/239‑1 (MQ=255)

                                                                                                                                                           aaaaagaagtCGAAGGCGATTG‑AG‑ATGAAAACCCGGGCGTTAA‑TC‑CCAA‑AAAGAAAAGTTTAAACCGGT‑TTTTGCGTTGTTTTAGA‑GCCACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGc                                                  <  1:284585/235‑1 (MQ=255)

                                                                                                                                                            aaaaaaagtggagtgtgATGG‑AG‑AAGAAAACACCGGGGGTGA‑‑CACCAGTATGA‑AAAGTTTGAAGCGTT‑GGTTTCTTTCCTTTAAA‑CACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGcc                                                 <  1:1219004/223‑1 (MQ=255)

                                                                                                                                                             atggaagaCGAATGCGATGG‑AG‑AGAAAAACCCGGCCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTAAGGCGGT‑TGTTTCTTTGTTTTATG‑ACCCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCt                                                <  2:506524/236‑1 (MQ=255)

                                                                                                                                                                   gaCGAATGCGCTTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑ACAACCTTAAGCGGA‑TGTTTCTTTGCTTTATA‑GACCCCCGCCTCTTCGGCCCCTTTGGCCATTTCAGCAGGAAGTAAAAATTTAAAAGGGTTGTCAGCTTTCCCACTAACTCTCTCTTTTTTAAGTCGGCGACGCGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCACGCCTGGCt                                            >  2:751079/1‑237 (MQ=255)

                                                                                                                                                                   gaCGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTta                                          >  1:1069068/1‑239 (MQ=255)

                                                                                                                                                                   gaCGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCCCTTCGGCCACTTTTGCCATTGCAGCAGGGAGTAAAAGATCAAACGGGTTGTCAGCTTTCACACTAACTCTCCCTTTATTAAGTCGCCGCAGGGATACTAACAAAGCATTATAGAAGAGAAATTGaaatttgtctcttttacacatcttc                                           >  1:1305198/1‑217 (MQ=255)

                                                                                                                                                                   gaCGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑AAAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGGTCAAAAGGGTTGTCAGATTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGATAATAACAAAGAATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTta                                          >  2:147667/1‑239 (MQ=255)

                                                                                                                                                                     gaagggggagtG‑AG‑ATG‑AAACACCGGCGGGAAGTCGCCAG‑AAAG‑AAAGTCTTAAGGGGA‑‑GTTTGCGTGTTTTAAAGGCCACCCGCCTCTGCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATATCGCCTGGCTta                                          <  1:1682161/227‑1 (MQ=255)

                                                                                                                                                                        aTGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGG‑TGTTTCGTTGATTTATA‑AACACCCGCCTCTTCGGCCAATTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGGTGTCAGCTTTCACACTAACCCTCCCTTTATTAAGTCGGGGACGAGATACTAACAAAGCATTATAGCTGAGAAATTGATTTAGATCATATCTTGCCTGGCATATAgg                                      >  1:357890/1‑238 (MQ=255)

                                                                                                                                                                        aTGCGATTG‑AG‑ATGAAAACACCGGCGGAAA‑TTGCCAG‑ATCG‑CAAGACTTAAGTGGT‑TGATTCCTTGCTTTATA‑ACCACCCGACTCTTCGGCCACTTTTGTCCTTGGATAAGGAAATACACAATCAAAGGGGTTTTTACAGTTACCACTTAAACTCTCTTTATTAAAACGGCGACGCGAAACTAACAAAGCATAATTACTGTCAAATTGGTATAGATCCTAtctctattctacatctta                                      >  1:926996/1‑224 (MQ=255)

                                                                                                                                                                               ttggagagaaaaaCCCCGGGGGTAA‑GCGCAAG‑AAAA‑AAAGTCTTAAGAGGA‑TGTTTCGTTGTTTAAAA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGt                                 <  2:1388968/229‑1 (MQ=255)

                                                                                                                                                                                g‑ag‑aTGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACt                             <  2:1069068/239‑1 (MQ=255)

                                                                                                                                                                                      taaaacccccGGCTGTAA‑TCGCCAG‑ATTA‑AATGTCTTAAGCGGT‑TGTTTTTTTGTTTTATA‑GCCCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  2:301342/231‑1 (MQ=255)

                                                                                                                                                                                      taaaaaCCCCGGCGTTAA‑CCGCCGG‑ATAG‑AAAGTCGAAAGCGGA‑TGTTTCGTTTCTTTAAA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  2:63770/237‑1 (MQ=255)

                                                                                                                                                                                      tGAAACCCCCGGGGGTAG‑CCGCGAG‑AGAG‑AAAGTCTTAAGTGGG‑TTTTGGTTTGCTTAAAA‑GACGTCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  2:357890/239‑1 (MQ=255)

                                                                                                                                                                                      agAAAACCCGGGGGTTAA‑CCCCAAG‑ATAG‑AAA‑TCTTTAGGGGATTTTTTCGTTGTTTTAAA‑CACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  1:1325284/238‑1 (MQ=255)

                                                                                                                                                                                       aaaacCACCGGCGGAAA‑TCGCAAG‑ATAG‑AAAGTCTAAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCAt                        <  2:882305/238‑1 (MQ=255)

                                                                                                                                                                                       aaaaaCCCCGGCGGTAA‑TCCCCGG‑ATAG‑AAAGTTTTAAGCGGA‑TGTTTCGTTGCTTTAAA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCAt                        <  2:1322375/238‑1 (MQ=255)

                                                                                                                                                                                               cgtgggTAA‑TCGCAGA‑ATAG‑AAAGTCTGAGGCGGA‑TTTTTCGTGGCTTTATA‑CACCACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTa                <  1:1189752/235‑1 (MQ=255)

                                                                                                                                                                                               cggcggTAA‑TCTCCAG‑ATAG‑AAAGTCTTAAGCGGG‑TGTTTCGTTGCTTTAAA‑AACACCCGCCTCTTCTTCCACTTTTGCCATTGCAGCAGGAAGTAAAAGAGCAAAAGGGTTGTTAGCTTTCACACTAACTCTCTCTTTATTAAATCGGCGACGAGACACTAACAAACCATTATAGATGTAAAATTGTTATTAGTCATATTTCGCCTGGCTTATAGGGCCGTGACACGCATGGTTTTTa                >  1:79010/1‑239 (MQ=255)

                                                                                                                                                                                                       a‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAAt         >  2:570452/1‑238 (MQ=255)

                                                                                                                                                                                                        agcGCCAG‑AAAG‑AAATTCTTAAGCGGT‑TGTTTGTTTACTTTGTA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  2:1040020/237‑1 (MQ=255)

                                                                                                                                                                                                        acccccAG‑ATAA‑AAAGTGTAAAGGGGA‑TGTTTGTTTTCTATAAA‑AACAACCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATa        <  1:1355827/235‑1 (MQ=255)

                                                                                                                                                                                                          tgcacg‑ATGG‑AAAGTTTAAGGCGGA‑TGTTTCGTTGCTTTAAA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAGGTAAAAGATCAAAAGGGTTGTCAGCTTTCTCACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACg      <  2:875911/234‑1 (MQ=255)

                                                                                                                                                                                                             cAG‑ATAA‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTAAA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAg   <  2:1649144/239‑1 (MQ=255)

                                                                                                                                                                                                              ag‑aaag‑aaATTCGTAAGCGGT‑TGTTGCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  2:1418791/239‑1 (MQ=255)

                                                                                                                                                                                                               aaaTAG‑AAGTTCTTAAGGGGA‑TGTTTCGTTGCTTAAGA‑GCAACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAg   <  1:792278/236‑1 (MQ=255)

                                                                                                                                                                                                                  tAG‑AAAGTCTTAGCCGGT‑TGTTTCGTTGCTTTAAA‑GACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  2:1411825/236‑1 (MQ=255)

                                                                                                                                                                                                                  tAG‑AAAGTCTTAAGCGGT‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  2:1334059/236‑1 (MQ=255)

                                                                                                                                                                                                                   taaaaaggttaaaGCGGA‑TTTTGCTTTGCTTTAGA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGt  <  1:774718/225‑1 (MQ=255)

                                                                                                                                                                                                                              aaGCGGA‑TGTTTCGTTGTTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         <  2:1074442/202‑1 (MQ=255)

                                                                                                                                                                                                                              aaGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCa                         >  1:1074442/1‑202 (MQ=255)

                                                                                                                                                                                                                                    a‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTAt               >  2:444793/1‑206 (MQ=255)

CCCACTCGCCTTAGCAACAACAATCAACACGGTGGAAGTAAAGAGATCGGCTCCGCAGACAACACAAAGAATCAGCCCCAGAGAGAAGCAAATG‑CCGCCAACCAGTTTTGCCATGCCGAAGGGCATTGTGCCTGT‑GCCA‑GTGGTTGCTGTGATAT‑AGAAGACGAATGCGATTG‑AG‑ATGAAAACACCGGCGGTAA‑TCGCCAG‑ATAG‑AAAGTCTTAAGCGGA‑TGTTTCGTTGCTTTATA‑GACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAAGTCGGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGT  >  NC_000913/954158‑954595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`